AT5G57010

Short calmodulin-binding motif containing conserved Ile and Gln residues.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0000760
Physical Location & Seq
Forward (+)
23067828 .. 23070431
2604 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G57010.1

Sequence Viewer

Length: 1488 bp
ATGGCTTTATCTTTTGGTTACTTACAAAGAGACAACAGTTTCAAGAAAGATTCACAAGAGTGCGAAACACCAAGAATTCGCAAAACCCCAGTAAACATGTATCTTGAGAAGACTTTATCGTTCAAAGATTTGGTCGATCAGAGGAACAATTACAGAGATGGAAATTGCGGGGTGAAAACGAGAAAAGGTATAAACTTGAAGGGTCCTAAACCAGATAACATGATTCTTGATCGGAGTCTCTCGTTCACGAGTCTAGTCCAAGTGGAAAACAGAGGAGGAGAAGAAGAAGACGAAAGAGGCTCGTCTCCGAAACGAAGGAACAGGGGTAACTTAACGGCGTTAAGTTTACCAGCACCGACGCCATTTTGGTCTCCGAGACCGTCCACGGAGCTAGACGCTGCCGCCGTTACGTTACAAAAGGTTTACAAGAGTTATCGAACGCGTAGAAATCTTGCTGACTGTGCAGTTGTTGTTGAAGAATTATGGTGGAAAGAATTGGAATTGGCAAAGTTAGAACCGAACAAAACCAATGATAAACCAGAGTCTGCTGTATCAAGGTGGGCAAGAGCTGGAACAAAAGCAGCAAAGGTGGGGAAAGGATTGTTGAAAGATGATAAAGCACAAAAATTAGCATTGCGACATTGGTTAGAAGCCATTGATCCACGACATAGGTACGGGCATAACTTACACTTATACTATGATGTCTGGTCAGAAAGTGAGAGTACTCAGCCATTTTTCTTCTGGTTAGACATTGGAGATGGCAAGGAAGTAAATCTCAACAAATGTTCAAGAACTCTTCTTCAGCGTCAATGCATCACTTACCTTGGTCCGAAAGAGAGACAAGCTTACGAAGTGGTCGTGGAAGATGGGAAGTTAGTCAGCAGACAAACCAAGAGCCTTGTGGAGACTACTGAAGGAACCAAATGGATCTTTGTGTTGAGCACGACAAGAAAACTGTACATTGGTCAGAAACAGAAAGGTCGGTTTCAACACTCGAGCTTTCTCTCCGGCGCCGCGATTACCGCCGCCGGTAGAATTGTTTCACACGATGGAGTTGTAAAAGCTGTGTGGCCGTACAGTGGTCATTATCTTCCGACGGAAGAGAATTTCAGAGAATTTATCTGTTTCTTAAGAGAAAACCACGTGAATCTCACTAATGTCAAGATGAATGCGATTGACGACGATGATCATCTGGTCAATAATGATGGAAGCACTAAGCCGTCGATGATGGTGGCGAAATCGGACGGTTCAGATGAACAGAAACGATTTTCATGCAAGTGGAGTACAGGAAACGGTCCAAGGATCGGGTGTGTGCGGGACTACCCAATGGATCTGCAGACGCGGGCGCTTGAACAAGTCAACCTTTCTCCTCGTGTAGTCAACGGAACAATGGGATTATTTGGCCCAATACCATCGCCAAGACCCAGCCCAAAGATTCGTGTCTCTCCTAGACTATCTTGCATGGGTCTTCCAAGCCCAAGGCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

495

Amino Acids

56.15

Weight (kDa)

9.44

Isoelectric Point (pI)

41.89

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1010
AccII CGCG 3 cut(s) 442, 1016, 1342
AciI CCGC 7 cut(s) 168, 402, 1014, 1023, 1026, 1315, 1342
AclWI GGATC 4 cut(s) 653, 935, 1310, 1338
AcoI YGGCCR 1 cut(s) 1070
AcsI RAATTY 3 cut(s) 75, 1105, 1115
AcuI CTGAAG 2 cut(s) 785, 933
AcvI CACGTG 1 cut(s) 1144
AcyI GRCGYC 2 cut(s) 359, 1011
AfaI GTAC 5 cut(s) 674, 724, 959, 1076, 1285
AfiI CCNNNNNNNGG 2 cut(s) 1079, 1304
AflII CTTAAG 1 cut(s) 1129
AflIII ACRYGT 2 cut(s) 96, 440
AgsI TTSAA 8 cut(s) 43, 124, 199, 476, 607, 789, 989, 1352
AleI CACNNNNGTG 1 cut(s) 58
AluBI AGCT 5 cut(s) 391, 569, 845, 999, 1064
AluI AGCT 5 cut(s) 391, 569, 845, 999, 1064
Alw21I GWGCWC 1 cut(s) 944
Alw26I GTCTC 8 cut(s) 24, 242, 309, 370, 375, 832, 899, 1447
AlwI GGATC 4 cut(s) 653, 935, 1310, 1338
AlwNI CAGNNNCTG 1 cut(s) 545
Ama87I CYCGRG 1 cut(s) 994
AoxI GGCC 2 cut(s) 1070, 1402
ApeKI GCWGC 2 cut(s) 398, 581
ApoI RAATTY 3 cut(s) 75, 1105, 1115
ArsI GACNNNNNNTTYG 2 cut(s) 117, 149
Asp700I GAANNNNTTC 1 cut(s) 1039
AspLEI GCGC 2 cut(s) 1013, 1348
AspS9I GGNCC 4 cut(s) 203, 827, 1295, 1403
AsuHPI GGTGA 1 cut(s) 184
AvaI CYCGRG 1 cut(s) 994
AvaII GGWCC 3 cut(s) 203, 827, 1295
BanI GGYRCC 1 cut(s) 1010
BauI CACGAG 2 cut(s) 247, 1371
BbrPI CACGTG 1 cut(s) 1144
BbsI GAAGAC 3 cut(s) 116, 294, 1460
Bbv12I GWGCWC 1 cut(s) 944
BbvI GCAGC 2 cut(s) 385, 593
BccI CCATC 7 cut(s) 152, 752, 860, 1043, 1199, 1222, 1420
BceAI ACGGC 4 cut(s) 351, 389, 1057, 1204
BcgI CGANNNNNNTGC 2 cut(s) 1254, 1288
BclI TGATCA 1 cut(s) 1186
BcoDI GTCTC 8 cut(s) 24, 242, 309, 370, 375, 832, 899, 1447
BfaI CTAG 3 cut(s) 254, 392, 1449
BfmI CTRYAG 1 cut(s) 1334
BfoI RGCGCY 2 cut(s) 1014, 1349
BfrI CTTAAG 1 cut(s) 1129
BisI GCNGC 5 cut(s) 399, 402, 582, 1014, 1026
BlsI GCNGC 5 cut(s) 400, 403, 583, 1015, 1027
BmcAI AGTACT 1 cut(s) 724
Bme18I GGWCC 3 cut(s) 203, 827, 1295
BmeT110I CYCGRG 1 cut(s) 994
BmgT120I GGNCC 4 cut(s) 203, 827, 1295, 1403
BmiI GGNNCC 3 cut(s) 204, 919, 1012
BmrI ACTGGG 1 cut(s) 83
BmsI GCATC 1 cut(s) 822
BmuI ACTGGG 1 cut(s) 83
BpiI GAAGAC 3 cut(s) 116, 294, 1460
BpuEI CTTGAG 1 cut(s) 125
BsaAI YACGTR 1 cut(s) 1144
BsaBI GATNNNNATC 1 cut(s) 1188
BsaHI GRCGYC 2 cut(s) 359, 1011
BsaI GGTCTC 2 cut(s) 370, 375
BsaJI CCNNGG 4 cut(s) 384, 823, 1298, 1478
Bsc4I CCNNNNNNNGG 2 cut(s) 1079, 1304
Bse118I RCCGGY 1 cut(s) 1028
Bse1I ACTGG 1 cut(s) 89
Bse3DI GCAATG 1 cut(s) 632
Bse8I GATNNNNATC 1 cut(s) 1188
BseDI CCNNGG 4 cut(s) 384, 823, 1298, 1478
BseJI GATNNNNATC 1 cut(s) 1188
BseLI CCNNNNNNNGG 2 cut(s) 1079, 1304
BseMI GCAATG 1 cut(s) 632
BseMII CTCAG 1 cut(s) 740
BseNI ACTGG 1 cut(s) 89
BseRI GAGGAG 3 cut(s) 288, 291, 1359
BseXI GCAGC 2 cut(s) 385, 593
BseYI CCCAGC 1 cut(s) 1424
BsgI GTGCAG 1 cut(s) 483
Bsh1236I CGCG 3 cut(s) 442, 1016, 1342
BshFI GGCC 2 cut(s) 1072, 1404
BshNI GGYRCC 1 cut(s) 1010
BsiHKAI GWGCWC 1 cut(s) 944
BsiHKCI CYCGRG 1 cut(s) 994
BsiSI CCGG 2 cut(s) 1008, 1029
BslFI GGGAC 1 cut(s) 1331
BslI CCNNNNNNNGG 2 cut(s) 1079, 1304
BsmAI GTCTC 8 cut(s) 24, 242, 309, 370, 375, 832, 899, 1447
BsmBI CGTCTC 1 cut(s) 309
BsmFI GGGAC 1 cut(s) 1331
BsmI GAATGC 1 cut(s) 1174
BsnI GGCC 2 cut(s) 1072, 1404
Bso31I GGTCTC 2 cut(s) 370, 375
BsoBI CYCGRG 1 cut(s) 994
Bsp1286I GDGCHC 1 cut(s) 944
Bsp1407I TGTACA 1 cut(s) 957
Bsp143I GATC 7 cut(s) 136, 229, 658, 927, 1186, 1302, 1330
BspACI CCGC 7 cut(s) 168, 402, 1014, 1023, 1026, 1315, 1342
BspANI GGCC 2 cut(s) 1072, 1404
BspCNI CTCAG 1 cut(s) 739
BspFNI CGCG 3 cut(s) 442, 1016, 1342
BspLI GGNNCC 3 cut(s) 204, 919, 1012
BspMAI CTGCAG 1 cut(s) 1338
BspPI GGATC 4 cut(s) 653, 935, 1310, 1338
BspT107I GGYRCC 1 cut(s) 1010
BspTI CTTAAG 1 cut(s) 1129
BspTNI GGTCTC 2 cut(s) 370, 375
BsrDI GCAATG 1 cut(s) 632
BsrFI RCCGGY 1 cut(s) 1028
BsrGI TGTACA 1 cut(s) 957
BsrI ACTGG 1 cut(s) 89
BssAI RCCGGY 1 cut(s) 1028
BssECI CCNNGG 4 cut(s) 384, 823, 1298, 1478
BssMI GATC 7 cut(s) 136, 229, 658, 927, 1186, 1302, 1330
BssNI GRCGYC 2 cut(s) 359, 1011
BssSI CACGAG 2 cut(s) 247, 1371
BssT1I CCWWGG 3 cut(s) 823, 1298, 1478
Bst2BI CACGAG 2 cut(s) 247, 1371
Bst4CI ACNGT 7 cut(s) 38, 381, 461, 957, 1079, 1247, 1295
Bst6I CTCTTC 2 cut(s) 801, 1095
BstACI GRCGYC 2 cut(s) 359, 1011
BstAFI CTTAAG 1 cut(s) 1129
BstAUI TGTACA 1 cut(s) 957
BstBAI YACGTR 1 cut(s) 1144
BstC8I GCNNGC 1 cut(s) 1344
BstDEI CTNAG 2 cut(s) 726, 1215
BstDSI CCRYGG 1 cut(s) 384
BstFNI CGCG 3 cut(s) 442, 1016, 1342
BstH2I RGCGCY 2 cut(s) 1014, 1349
BstHHI GCGC 2 cut(s) 1013, 1348
BstKTI GATC 7 cut(s) 139, 232, 661, 930, 1189, 1305, 1333
BstMAI GTCTC 8 cut(s) 24, 242, 309, 370, 375, 832, 899, 1447
BstMBI GATC 7 cut(s) 136, 229, 658, 927, 1186, 1302, 1330
BstMWI GCNNNNNNNGC 2 cut(s) 461, 1022
BstNSI RCATGY 1 cut(s) 100
BstSFI CTRYAG 1 cut(s) 1334
BstUI CGCG 3 cut(s) 442, 1016, 1342
BstV1I GCAGC 2 cut(s) 385, 593
BstV2I GAAGAC 3 cut(s) 116, 294, 1460
BstX2I RGATCY 2 cut(s) 927, 1330
BstYI RGATCY 2 cut(s) 927, 1330
BsuRI GGCC 2 cut(s) 1072, 1404
BtgI CCRYGG 1 cut(s) 384
BtgZI GCGATG 1 cut(s) 1398
BtsIMutI CAGTG 1 cut(s) 1084
Cac8I GCNNGC 1 cut(s) 1344
CaiI CAGNNNCTG 1 cut(s) 545
CfoI GCGC 2 cut(s) 1013, 1348
Cfr10I RCCGGY 1 cut(s) 1028
Cfr13I GGNCC 4 cut(s) 203, 827, 1295, 1403
CseI GACGC 4 cut(s) 367, 404, 794, 1348
Csp6I GTAC 5 cut(s) 673, 723, 958, 1075, 1284
CviAII CATG 4 cut(s) 97, 220, 1272, 1462
CviQI GTAC 5 cut(s) 673, 723, 958, 1075, 1284
DdeI CTNAG 2 cut(s) 726, 1215
DinI GGCGCC 1 cut(s) 1012
DpnI GATC 7 cut(s) 138, 231, 660, 929, 1188, 1304, 1332
DpnII GATC 7 cut(s) 136, 229, 658, 927, 1186, 1302, 1330
EaeI YGGCCR 1 cut(s) 1070
Eam1104I CTCTTC 2 cut(s) 801, 1095
EarI CTCTTC 2 cut(s) 801, 1095
Eco130I CCWWGG 3 cut(s) 823, 1298, 1478
Eco31I GGTCTC 2 cut(s) 370, 375
Eco47I GGWCC 3 cut(s) 203, 827, 1295
Eco57I CTGAAG 2 cut(s) 785, 933
Eco72I CACGTG 1 cut(s) 1144
Eco88I CYCGRG 1 cut(s) 994
EcoO109I RGGNCCY 1 cut(s) 203
EcoRI GAATTC 1 cut(s) 75
EcoT14I CCWWGG 3 cut(s) 823, 1298, 1478
EcoT22I ATGCAT 1 cut(s) 815
EgeI GGCGCC 1 cut(s) 1012
EheI GGCGCC 1 cut(s) 1012
ErhI CCWWGG 3 cut(s) 823, 1298, 1478
Esp3I CGTCTC 1 cut(s) 309
FaeI CATG 4 cut(s) 100, 223, 1275, 1465
FalI AAGNNNNNCTT 2 cut(s) 1347, 1379
FaqI GGGAC 1 cut(s) 1331
FatI CATG 4 cut(s) 96, 219, 1271, 1461
FauI CCCGC 3 cut(s) 161, 1308, 1335
FbaI TGATCA 1 cut(s) 1186
Fnu4HI GCNGC 5 cut(s) 399, 402, 582, 1014, 1026
Fsp4HI GCNGC 5 cut(s) 399, 402, 582, 1014, 1026
FspBI CTAG 3 cut(s) 254, 392, 1449
GlaI GCGC 2 cut(s) 1012, 1347
GluI GCNGC 5 cut(s) 399, 402, 582, 1014, 1026
GsaI CCCAGC 1 cut(s) 1428
HaeII RGCGCY 2 cut(s) 1014, 1349
HaeIII GGCC 2 cut(s) 1072, 1404
HapII CCGG 2 cut(s) 1008, 1029
HgaI GACGC 4 cut(s) 367, 404, 794, 1348
HhaI GCGC 2 cut(s) 1013, 1348
Hin1I GRCGYC 2 cut(s) 359, 1011
Hin1II CATG 4 cut(s) 100, 223, 1275, 1465
Hin6I GCGC 2 cut(s) 1011, 1346
HinP1I GCGC 2 cut(s) 1011, 1346
HincII GTYRAC 2 cut(s) 1360, 1381
HindII GTYRAC 2 cut(s) 1360, 1381
HindIII AAGCTT 1 cut(s) 843
HinfI GANTC 7 cut(s) 50, 223, 235, 250, 542, 1147, 1435
HpaII CCGG 2 cut(s) 1008, 1029
HphI GGTGA 1 cut(s) 184
Hpy166II GTNNAC 7 cut(s) 94, 246, 347, 384, 424, 1360, 1381
Hpy188III TCNNGA 6 cut(s) 43, 104, 227, 247, 789, 1162
Hpy8I GTNNAC 7 cut(s) 94, 246, 347, 384, 424, 1360, 1381
Hpy99I CGWCG 4 cut(s) 361, 1099, 1184, 1225
HpyAV CCTTC 3 cut(s) 193, 309, 908
HpyCH4III ACNGT 7 cut(s) 38, 381, 461, 957, 1079, 1247, 1295
HpyCH4IV ACGT 2 cut(s) 410, 1143
HpyCH4V TGCA 5 cut(s) 464, 813, 1275, 1336, 1461
HpyF10VI GCNNNNNNNGC 2 cut(s) 461, 1022
HpyF3I CTNAG 2 cut(s) 726, 1215
HpySE526I ACGT 2 cut(s) 410, 1143
Hsp92I GRCGYC 2 cut(s) 359, 1011
Hsp92II CATG 4 cut(s) 100, 223, 1275, 1465
HspAI GCGC 2 cut(s) 1011, 1346
KasI GGCGCC 1 cut(s) 1010
Ksp22I TGATCA 1 cut(s) 1186
Kzo9I GATC 7 cut(s) 136, 229, 658, 927, 1186, 1302, 1330
LmnI GCTCC 1 cut(s) 388
Lsp1109I GCAGC 2 cut(s) 385, 593
LweI GCATC 1 cut(s) 822
MaeI CTAG 3 cut(s) 254, 392, 1449
MaeII ACGT 2 cut(s) 410, 1143
MaeIII GTNAC 4 cut(s) 17, 326, 406, 411
MalI GATC 7 cut(s) 138, 231, 660, 929, 1188, 1304, 1332
MboI GATC 7 cut(s) 136, 229, 658, 927, 1186, 1302, 1330
MflI RGATCY 2 cut(s) 927, 1330
MhlI GDGCHC 1 cut(s) 944
MluI ACGCGT 1 cut(s) 440
Mly113I GGCGCC 1 cut(s) 1011
MlyI GAGTC 3 cut(s) 244, 259, 551
MmeI TCCRAC 1 cut(s) 1118
MnlI CCTC 5 cut(s) 135, 266, 269, 290, 1380
Mph1103I ATGCAT 1 cut(s) 815
MroXI GAANNNNTTC 1 cut(s) 1039
MseI TTAA 3 cut(s) 332, 341, 1130
MslI CAYNNNNRTG 2 cut(s) 58, 1276
MspCI CTTAAG 1 cut(s) 1129
MspI CCGG 2 cut(s) 1008, 1029
Mva1269I GAATGC 1 cut(s) 1174
MvnI CGCG 3 cut(s) 442, 1016, 1342
MwoI GCNNNNNNNGC 2 cut(s) 461, 1022
NarI GGCGCC 1 cut(s) 1011
NdeII GATC 7 cut(s) 136, 229, 658, 927, 1186, 1302, 1330
NlaIII CATG 4 cut(s) 100, 223, 1275, 1465
NlaIV GGNNCC 3 cut(s) 204, 919, 1012
NsiI ATGCAT 1 cut(s) 815
NspI RCATGY 1 cut(s) 100
OliI CACNNNNGTG 1 cut(s) 58
PaeR7I CTCGAG 1 cut(s) 994
PciI ACATGT 1 cut(s) 96
PcsI WCGNNNNNNNCGW 2 cut(s) 402, 855
PctI GAATGC 1 cut(s) 1174
PdmI GAANNNNTTC 1 cut(s) 1039
PfeI GAWTC 4 cut(s) 50, 223, 1147, 1435
PkrI GCNGC 5 cut(s) 400, 403, 583, 1015, 1027
PleI GAGTC 3 cut(s) 243, 258, 550
PluTI GGCGCC 1 cut(s) 1014
PmaCI CACGTG 1 cut(s) 1144
PmlI CACGTG 1 cut(s) 1144
PpsI GAGTC 3 cut(s) 243, 258, 550
Ppu21I YACGTR 1 cut(s) 1144
PpuMI RGGWCCY 1 cut(s) 203
PscI ACATGT 1 cut(s) 96
Psp5II RGGWCCY 1 cut(s) 203
PspCI CACGTG 1 cut(s) 1144
PspFI CCCAGC 1 cut(s) 1424
PspN4I GGNNCC 3 cut(s) 204, 919, 1012
PspPI GGNCC 4 cut(s) 203, 827, 1295, 1403
PspPPI RGGWCCY 1 cut(s) 203
PspXI VCTCGAGB 1 cut(s) 994
PstI CTGCAG 1 cut(s) 1338
PstNI CAGNNNCTG 1 cut(s) 545
PsuI RGATCY 2 cut(s) 927, 1330
RsaI GTAC 5 cut(s) 674, 724, 959, 1076, 1285
RsaNI GTAC 5 cut(s) 673, 723, 958, 1075, 1284
RseI CAYNNNNRTG 2 cut(s) 58, 1276
SaqAI TTAA 3 cut(s) 332, 341, 1130
SatI GCNGC 5 cut(s) 399, 402, 582, 1014, 1026
Sau3AI GATC 7 cut(s) 136, 229, 658, 927, 1186, 1302, 1330
Sau96I GGNCC 4 cut(s) 203, 827, 1295, 1403
ScaI AGTACT 1 cut(s) 724
SchI GAGTC 3 cut(s) 244, 259, 551
SduI GDGCHC 1 cut(s) 944
SfaNI GCATC 1 cut(s) 822
SfcI CTRYAG 1 cut(s) 1334
SfoI GGCGCC 1 cut(s) 1012
Sfr274I CTCGAG 1 cut(s) 994
SinI GGWCC 3 cut(s) 203, 827, 1295
SlaI CTCGAG 1 cut(s) 994
SmiMI CAYNNNNRTG 2 cut(s) 58, 1276
SmlI CTYRAG 3 cut(s) 104, 994, 1129
SmoI CTYRAG 3 cut(s) 104, 994, 1129
SsiI CCGC 7 cut(s) 168, 402, 1014, 1023, 1026, 1315, 1342
SspDI GGCGCC 1 cut(s) 1010
SspMI CTAG 3 cut(s) 254, 392, 1449
StyI CCWWGG 3 cut(s) 823, 1298, 1478
TaaI ACNGT 7 cut(s) 38, 381, 461, 957, 1079, 1247, 1295
TaiI ACGT 2 cut(s) 413, 1146
TaqI TCGA 4 cut(s) 135, 436, 995, 1223
TatI WGTACW 3 cut(s) 722, 957, 1283
TauI GCSGC 3 cut(s) 404, 1016, 1028
TfiI GAWTC 4 cut(s) 50, 223, 1147, 1435
Tru1I TTAA 3 cut(s) 332, 341, 1130
Tru9I TTAA 3 cut(s) 332, 341, 1130
TscAI CASTG 1 cut(s) 1084
TseI GCWGC 2 cut(s) 398, 581
TspDTI ATGAA 3 cut(s) 1181, 1260, 1269
TspGWI ACGGA 3 cut(s) 401, 1112, 1398
TspRI CASTG 1 cut(s) 1084
Vha464I CTTAAG 1 cut(s) 1129
VpaK11BI GGWCC 3 cut(s) 203, 827, 1295
XapI RAATTY 3 cut(s) 75, 1105, 1115
XceI RCATGY 1 cut(s) 100
XcmI CCANNNNNNNNNTGG 2 cut(s) 738, 898
XhoI CTCGAG 1 cut(s) 994
XmnI GAANNNNTTC 1 cut(s) 1039
XspI CTAG 3 cut(s) 254, 392, 1449
ZrmI AGTACT 1 cut(s) 724
Zsp2I ATGCAT 1 cut(s) 815
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.