MD09G1190600.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000760
Physical Location & Seq
Forward (+)
16656740 .. 16658838
2099 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1190600.v1.1.491

Sequence Viewer

Length: 1104 bp
ATGGGCTCGCAGACATCCACCAATTTGAATTCCAAGAGGGCAACGCCTAGAACAATTAGCTTCAAGAAAACTGATTTAAGCAAAACTGGAATATCTGATGGGATGGAAAAAGTGCTTTTTGAAGAATCTATAAGCAGCAAGAAAAGGAAACTAGGTGATCAGCTAAATATTGGGACAAATGTTTCAAGGAAAACTGCAGAGGGAAAAACTGAAATTTTCATTGAAAAGTTAAAGCCAGAAGATTGGGTTTCTCCACGGTCTCCAATTGAGCTTGATGCAGCTGCAAGCAAGGTGCAGAAAGTTTACAAGAGTTACAGGACTAGACGAAACCTTGCAGATTGCGCTGTTGTTGTTGAGGAGCTATGGTGGCAGGCATTGGACTTTGCTGCTCTTAGCAGGAGCTCCGTGTCGTTTTTTGAATCTGTTAAATCAGAAACTGCAGTTTCAAGATGGGCAAGGGCTAGGACTAGGGCTGCCAAGGTTGGGAAGGGTTTGTCCATGGACGAGAAGACTCCGAAATTAGCTCTAATACATTGGCTTGAGGCTATTGATCCTCGCCATCGGTATGGACAAAATCTGCACTTGTACCATGATGTTTGGTTTAATAGTGGGAGCTTTCAGCCTTTCTTCTACTGGATTCCTTCGCAATTTTTGATGCTCTGTCCGGAACGAAGGTTGGATGTTGGTGATGGTAAAGAAGTAAACCTTGACAAGTGCCCAAGAACTGATCTGCAGTCTCAATGCATCAAATACCTTGGACCAAGGGAAGCTTATGAAGTGATTGTGGAGAGTAGGAAGCTTGTGTACAGACAAAGTGGGAAACTTGTGAATACTCGTGAGGGTTCTAAGTGGATCTTTGTCCTCAGCACATCAAGGAATCTGTATGTTGGGGAGAAGAAGAAAGGCTTTTTCCAGCACTCTACTTTTCTTGCTGGAGGGGCCACCATTGCAGCAGGGAGAATTGTAGCCTCTAATGGGCTTCCTGAGGCTGTATGGCCTTACAGCGGTCATTACTGCCCGACAAAAGAGAATTTTATGGAATTCATCAGCTTTTTAGAGGAGCATCAGGTGGACTTGGCTGATGTCAAAGTAAATTATTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IQ

Protein Analysis

368

Amino Acids

41.72

Weight (kDa)

9.39

Isoelectric Point (pI)

35.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 664
AciI CCGC 1 cut(s) 1005
AclWI GGATC 2 cut(s) 545, 860
AcsI RAATTY 4 cut(s) 28, 213, 1030, 1040
AfaI GTAC 2 cut(s) 587, 806
AfiI CCNNNNNNNGG 3 cut(s) 483, 975, 1004
AgsI TTSAA 7 cut(s) 28, 64, 122, 186, 224, 419, 447
Alw21I GWGCWC 1 cut(s) 404
Alw26I GTCTC 2 cut(s) 264, 741
AlwI GGATC 2 cut(s) 545, 860
AlwNI CAGNNNCTG 1 cut(s) 437
Aor13HI TCCGGA 1 cut(s) 664
AoxI GGCC 2 cut(s) 939, 995
ApeKI GCWGC 6 cut(s) 135, 278, 281, 386, 473, 950
ApoI RAATTY 4 cut(s) 28, 213, 1030, 1040
AspLEI GCGC 1 cut(s) 344
AspS9I GGNCC 2 cut(s) 758, 939
AsuHPI GGTGA 2 cut(s) 167, 698
AvaII GGWCC 1 cut(s) 758
AxyI CCTNAGG 1 cut(s) 984
BaeGI GKGCMC 1 cut(s) 719
BanII GRGCYC 2 cut(s) 8, 404
BarI GAAGNNNNNNTAC 2 cut(s) 788, 820
BauI CACGAG 1 cut(s) 834
BbsI GAAGAC 1 cut(s) 515
Bbv12I GWGCWC 1 cut(s) 404
BbvCI CCTCAGC 1 cut(s) 863
BbvI GCAGC 6 cut(s) 147, 268, 290, 373, 460, 962
BccI CCATC 5 cut(s) 92, 97, 444, 567, 683
BclI TGATCA 1 cut(s) 157
BcoDI GTCTC 2 cut(s) 264, 741
BfaI CTAG 5 cut(s) 48, 152, 321, 462, 468
BfmI CTRYAG 3 cut(s) 195, 438, 731
BisI GCNGC 6 cut(s) 136, 279, 282, 387, 474, 951
BlsI GCNGC 6 cut(s) 137, 280, 283, 388, 475, 952
Bme18I GGWCC 1 cut(s) 758
BmgT120I GGNCC 2 cut(s) 758, 939
BmiI GGNNCC 1 cut(s) 940
BmsI GCATC 4 cut(s) 265, 645, 753, 1072
BpiI GAAGAC 1 cut(s) 515
BpmI CTGGAG 1 cut(s) 954
Bpu10I CCTNAGC 1 cut(s) 863
BpuEI CTTGAG 1 cut(s) 560
BsaI GGTCTC 1 cut(s) 264
BsaJI CCNNGG 5 cut(s) 254, 477, 498, 754, 761
BsaWI WCCGGW 1 cut(s) 664
BsaXI ACNNNNNCTCC 2 cut(s) 350, 380
Bsc4I CCNNNNNNNGG 3 cut(s) 483, 975, 1004
Bse1I ACTGG 2 cut(s) 91, 638
Bse21I CCTNAGG 1 cut(s) 984
Bse3DI GCAATG 1 cut(s) 945
BseAI TCCGGA 1 cut(s) 664
BseDI CCNNGG 5 cut(s) 254, 477, 498, 754, 761
BseGI GGATG 3 cut(s) 14, 108, 685
BseLI CCNNNNNNNGG 3 cut(s) 483, 975, 1004
BseMI GCAATG 1 cut(s) 945
BseMII CTCAG 2 cut(s) 877, 975
BseNI ACTGG 2 cut(s) 91, 638
BseRI GAGGAG 2 cut(s) 371, 1073
BseSI GKGCMC 1 cut(s) 719
BseXI GCAGC 6 cut(s) 147, 268, 290, 373, 460, 962
BsgI GTGCAG 2 cut(s) 314, 563
BshFI GGCC 2 cut(s) 941, 997
BsiHKAI GWGCWC 1 cut(s) 404
BsiSI CCGG 1 cut(s) 665
BslFI GGGAC 1 cut(s) 187
BslI CCNNNNNNNGG 3 cut(s) 483, 975, 1004
BsmAI GTCTC 2 cut(s) 264, 741
BsmFI GGGAC 1 cut(s) 187
BsnI GGCC 2 cut(s) 941, 997
Bso31I GGTCTC 1 cut(s) 264
Bsp1286I GDGCHC 3 cut(s) 8, 404, 719
Bsp13I TCCGGA 1 cut(s) 664
Bsp1407I TGTACA 1 cut(s) 804
Bsp143I GATC 4 cut(s) 157, 550, 727, 852
Bsp19I CCATGG 1 cut(s) 498
BspACI CCGC 1 cut(s) 1005
BspANI GGCC 2 cut(s) 941, 997
BspCNI CTCAG 2 cut(s) 876, 976
BspEI TCCGGA 1 cut(s) 664
BspLI GGNNCC 1 cut(s) 940
BspMAI CTGCAG 3 cut(s) 199, 442, 735
BspPI GGATC 2 cut(s) 545, 860
BspTNI GGTCTC 1 cut(s) 264
BsrDI GCAATG 1 cut(s) 945
BsrGI TGTACA 1 cut(s) 804
BsrI ACTGG 2 cut(s) 91, 638
BssECI CCNNGG 5 cut(s) 254, 477, 498, 754, 761
BssMI GATC 4 cut(s) 157, 550, 727, 852
BssSI CACGAG 1 cut(s) 834
BssT1I CCWWGG 4 cut(s) 477, 498, 754, 761
Bst2BI CACGAG 1 cut(s) 834
Bst4CI ACNGT 1 cut(s) 258
BstAUI TGTACA 1 cut(s) 804
BstC8I GCNNGC 3 cut(s) 8, 286, 372
BstDEI CTNAG 4 cut(s) 392, 846, 863, 984
BstDSI CCRYGG 2 cut(s) 254, 498
BstF5I GGATG 3 cut(s) 14, 108, 685
BstHHI GCGC 1 cut(s) 344
BstKTI GATC 4 cut(s) 160, 553, 730, 855
BstMAI GTCTC 2 cut(s) 264, 741
BstMBI GATC 4 cut(s) 157, 550, 727, 852
BstMWI GCNNNNNNNGC 4 cut(s) 341, 367, 938, 947
BstSFI CTRYAG 3 cut(s) 195, 438, 731
BstSLI GKGCMC 1 cut(s) 719
BstV1I GCAGC 6 cut(s) 147, 268, 290, 373, 460, 962
BstV2I GAAGAC 1 cut(s) 515
BstX2I RGATCY 1 cut(s) 852
BstXI CCANNNNNNTGG 2 cut(s) 243, 566
BstYI RGATCY 1 cut(s) 852
Bsu36I CCTNAGG 1 cut(s) 984
BsuRI GGCC 2 cut(s) 941, 997
BtgI CCRYGG 2 cut(s) 254, 498
BtsCI GGATG 3 cut(s) 14, 108, 685
Cac8I GCNNGC 3 cut(s) 8, 286, 372
CaiI CAGNNNCTG 1 cut(s) 437
CfoI GCGC 1 cut(s) 344
Cfr13I GGNCC 2 cut(s) 758, 939
Csp6I GTAC 2 cut(s) 586, 805
CviAII CATG 2 cut(s) 499, 590
CviQI GTAC 2 cut(s) 586, 805
DdeI CTNAG 4 cut(s) 392, 846, 863, 984
DpnI GATC 4 cut(s) 159, 552, 729, 854
DpnII GATC 4 cut(s) 157, 550, 727, 852
Ecl136II GAGCTC 1 cut(s) 402
Eco130I CCWWGG 4 cut(s) 477, 498, 754, 761
Eco24I GRGCYC 2 cut(s) 8, 404
Eco31I GGTCTC 1 cut(s) 264
Eco47I GGWCC 1 cut(s) 758
Eco53kI GAGCTC 1 cut(s) 402
Eco81I CCTNAGG 1 cut(s) 984
EcoICRI GAGCTC 1 cut(s) 402
EcoRI GAATTC 2 cut(s) 28, 1040
EcoT14I CCWWGG 4 cut(s) 477, 498, 754, 761
EcoT22I ATGCAT 1 cut(s) 746
EcoT38I GRGCYC 2 cut(s) 8, 404
ErhI CCWWGG 4 cut(s) 477, 498, 754, 761
FaeI CATG 2 cut(s) 502, 593
FaiI YATR 9 cut(s) 131, 364, 500, 567, 591, 774, 885, 994, 1037
FalI AAGNNNNNCTT 8 cut(s) 690, 722, 754, 786, 839, 871, 890, 922
FaqI GGGAC 1 cut(s) 187
FatI CATG 2 cut(s) 498, 589
FbaI TGATCA 1 cut(s) 157
Fnu4HI GCNGC 6 cut(s) 136, 279, 282, 387, 474, 951
FokI GGATG 2 cut(s) 115, 692
FriOI GRGCYC 2 cut(s) 8, 404
Fsp4HI GCNGC 6 cut(s) 136, 279, 282, 387, 474, 951
FspBI CTAG 5 cut(s) 48, 152, 321, 462, 468
GlaI GCGC 1 cut(s) 343
GluI GCNGC 6 cut(s) 136, 279, 282, 387, 474, 951
GsuI CTGGAG 1 cut(s) 954
HaeIII GGCC 2 cut(s) 941, 997
HapII CCGG 1 cut(s) 665
HhaI GCGC 1 cut(s) 344
Hin1II CATG 2 cut(s) 502, 593
Hin6I GCGC 1 cut(s) 342
HinP1I GCGC 1 cut(s) 342
HindIII AAGCTT 2 cut(s) 768, 797
HinfI GANTC 5 cut(s) 125, 419, 511, 637, 877
HpaII CCGG 1 cut(s) 665
HphI GGTGA 2 cut(s) 167, 698
Hpy166II GTNNAC 4 cut(s) 304, 703, 805, 1072
Hpy188I TCNGA 3 cut(s) 97, 433, 516
Hpy188III TCNNGA 5 cut(s) 64, 447, 665, 836, 983
Hpy8I GTNNAC 4 cut(s) 304, 703, 805, 1072
HpyAV CCTTC 3 cut(s) 481, 651, 666
HpyCH4III ACNGT 1 cut(s) 258
HpyF10VI GCNNNNNNNGC 4 cut(s) 341, 367, 938, 947
HpyF3I CTNAG 4 cut(s) 392, 846, 863, 984
Hsp92II CATG 2 cut(s) 502, 593
HspAI GCGC 1 cut(s) 342
Kpn2I TCCGGA 1 cut(s) 664
Ksp22I TGATCA 1 cut(s) 157
Kzo9I GATC 4 cut(s) 157, 550, 727, 852
LmnI GCTCC 5 cut(s) 358, 399, 407, 612, 1060
Lsp1109I GCAGC 6 cut(s) 147, 268, 290, 373, 460, 962
LweI GCATC 4 cut(s) 265, 645, 753, 1072
MaeI CTAG 5 cut(s) 48, 152, 321, 462, 468
MaeIII GTNAC 1 cut(s) 311
MalI GATC 4 cut(s) 159, 552, 729, 854
MboI GATC 4 cut(s) 157, 550, 727, 852
MboII GAAGA 6 cut(s) 134, 251, 520, 619, 907, 910
MfeI CAATTG 1 cut(s) 264
MflI RGATCY 1 cut(s) 852
MhlI GDGCHC 3 cut(s) 8, 404, 719
MlyI GAGTC 1 cut(s) 505
MmeI TCCRAC 1 cut(s) 657
Mph1103I ATGCAT 1 cut(s) 746
MroI TCCGGA 1 cut(s) 664
MseI TTAA 5 cut(s) 77, 230, 426, 603, 1102
MslI CAYNNNNRTG 1 cut(s) 564
MspA1I CMGCKG 2 cut(s) 281, 1005
MspI CCGG 1 cut(s) 665
MunI CAATTG 1 cut(s) 264
MwoI GCNNNNNNNGC 4 cut(s) 341, 367, 938, 947
NcoI CCATGG 1 cut(s) 498
NdeII GATC 4 cut(s) 157, 550, 727, 852
NlaIII CATG 2 cut(s) 502, 593
NlaIV GGNNCC 1 cut(s) 940
NsiI ATGCAT 1 cut(s) 746
PfeI GAWTC 4 cut(s) 125, 419, 637, 877
PkrI GCNGC 6 cut(s) 137, 280, 283, 388, 475, 952
PleI GAGTC 1 cut(s) 505
PpsI GAGTC 1 cut(s) 505
Psp124BI GAGCTC 1 cut(s) 404
PspN4I GGNNCC 1 cut(s) 940
PspPI GGNCC 2 cut(s) 758, 939
PstI CTGCAG 3 cut(s) 199, 442, 735
PstNI CAGNNNCTG 1 cut(s) 437
PsuI RGATCY 1 cut(s) 852
PvuII CAGCTG 1 cut(s) 281
RsaI GTAC 2 cut(s) 587, 806
RsaNI GTAC 2 cut(s) 586, 805
RseI CAYNNNNRTG 1 cut(s) 564
SacI GAGCTC 1 cut(s) 404
SaqAI TTAA 5 cut(s) 77, 230, 426, 603, 1102
SatI GCNGC 6 cut(s) 136, 279, 282, 387, 474, 951
Sau3AI GATC 4 cut(s) 157, 550, 727, 852
Sau96I GGNCC 2 cut(s) 758, 939
SchI GAGTC 1 cut(s) 505
SduI GDGCHC 3 cut(s) 8, 404, 719
SfaNI GCATC 4 cut(s) 265, 645, 753, 1072
SfcI CTRYAG 3 cut(s) 195, 438, 731
SinI GGWCC 1 cut(s) 758
SmiMI CAYNNNNRTG 1 cut(s) 564
SmlI CTYRAG 1 cut(s) 539
SmoI CTYRAG 1 cut(s) 539
SsiI CCGC 1 cut(s) 1005
SspI AATATT 1 cut(s) 169
SspMI CTAG 5 cut(s) 48, 152, 321, 462, 468
SstI GAGCTC 1 cut(s) 404
StyI CCWWGG 4 cut(s) 477, 498, 754, 761
TaaI ACNGT 1 cut(s) 258
TatI WGTACW 1 cut(s) 804
TfiI GAWTC 4 cut(s) 125, 419, 637, 877
Tru1I TTAA 5 cut(s) 77, 230, 426, 603, 1102
Tru9I TTAA 5 cut(s) 77, 230, 426, 603, 1102
TseI GCWGC 6 cut(s) 135, 278, 281, 386, 473, 950
TspDTI ATGAA 3 cut(s) 208, 789, 1033
TspGWI ACGGA 1 cut(s) 394
VpaK11BI GGWCC 1 cut(s) 758
XapI RAATTY 4 cut(s) 28, 213, 1030, 1040
XspI CTAG 5 cut(s) 48, 152, 321, 462, 468
Zsp2I ATGCAT 1 cut(s) 746
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.