pycom09g10700

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0000760
Physical Location & Seq
Forward (+)
8794159 .. 8796491
2333 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g10700.5

Sequence Viewer

Length: 1164 bp
ATGGGCTCGCAGACATCCACCAATATGAATTCCAAGAGGAAGGCAACGCCTAGAACAATTAGCTTCAAGAAAACTGATTTAAGCAAAACTGGAATATCCGATGTGATGGAAAAAGTGCTTTTTGAAGAATCTATAAGCAGCAAGAAAAGGAAACTAGGTGATCAGCTGAATATTGGGACAAATGTTTCAAGGAAAACTGCACAGGGAAAAACTGAAATTTTCATTGAAAAGTTAAAGCCAGAAGATTGGGTTTCTCCGCGGTCTCCAATTGAGCTTGATGCAGCTGCAATCAAGGTGCAGAAAGTTTACAAGAGTTACAGGACTAGACGAAACCTTGCAGATTGCGCTGTTGTTGTTGGGGAGCTATGGTGGCAGGCATTGGACTTTGCTGCTCTTAGACGGAGCTCCGTGTCGTTTTTTGAATCTATTAAATCAGAAACTGCAGTTTCAAGATGGGCAAGGGCTAGGACTAGGGCTGCCAAGGTTGGGAAGGGTTTGTCCACGGACGAGAAGACTCCGAAATTAGCTCTCAGACATTGGCTTGAGGCTATTGATCCTCGCCATCGGTATGGACAAAATTTGCACTTGTACTATGATGTCTGGTTTAATAGTGGGAGCTTTCAGCCTTTCTTCTACTGGTTGGATGTTGGTGATGGCAAAGAAGTAAACCTTGACAAGTGCCCAAGAACTGATCTGCAGTCTCAATGCATCAAATACCTTGGACCAAAAGAGAGGGAAGCTTATGAAGTGATTGTGGAGAGTGGGAAGCTTGTGTACAGACAAAGTGGGAAACTTGTGAATACTCGTGAGGGTTCTAAGTGGATCTTTGTCCTCAGCACATCAAGGAATATGTATGTTGGGGAGAAGAAGAAAGGCTTTTTCCAGCACTCTACTTTTCTTGCTGGAGGGGCCACCATCGCAGCAGGGAGAGTTGTAGCCTCTAATGGGTTTCCTGAGGCTGTATGGCCTTACAGCGGTCATTACTGCCCGACAAAAGAGAATTTTATGGAATTCATCAGCTTTTTAGAGGAGCATCAGGTGGACTTGGCTGATGTCAAAACATACCCTATCGATGACGATGTTCCACCATCATATGCTACCAAGAATGATCACGTGAAGTCAAAGTCTATATCAAGCACCCTTGAAGATATCCCCATCTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IQ

Protein Analysis

388

Amino Acids

43.87

Weight (kDa)

9.32

Isoelectric Point (pI)

36.39

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 259
AciI CCGC 3 cut(s) 257, 259, 975
AclWI GGATC 2 cut(s) 548, 830
AcsI RAATTY 5 cut(s) 28, 216, 577, 1000, 1010
AcvI CACGTG 1 cut(s) 1114
AfaI GTAC 2 cut(s) 590, 776
AfiI CCNNNNNNNGG 3 cut(s) 486, 945, 974
AgsI TTSAA 7 cut(s) 67, 125, 189, 227, 422, 450, 1145
Alw21I GWGCWC 1 cut(s) 407
Alw26I GTCTC 2 cut(s) 267, 705
AlwI GGATC 2 cut(s) 548, 830
AlwNI CAGNNNCTG 1 cut(s) 440
AoxI GGCC 2 cut(s) 909, 965
ApeKI GCWGC 6 cut(s) 138, 281, 284, 389, 476, 920
ApoI RAATTY 5 cut(s) 28, 216, 577, 1000, 1010
AspLEI GCGC 1 cut(s) 347
AspS9I GGNCC 2 cut(s) 722, 909
AsuHPI GGTGA 2 cut(s) 170, 662
AvaII GGWCC 1 cut(s) 722
AxyI CCTNAGG 1 cut(s) 954
BaeGI GKGCMC 1 cut(s) 683
BanII GRGCYC 2 cut(s) 8, 407
BarI GAAGNNNNNNTAC 2 cut(s) 758, 790
BauI CACGAG 1 cut(s) 804
BbrPI CACGTG 1 cut(s) 1114
BbsI GAAGAC 1 cut(s) 518
Bbv12I GWGCWC 1 cut(s) 407
BbvCI CCTCAGC 1 cut(s) 833
BbvI GCAGC 6 cut(s) 150, 271, 293, 376, 463, 932
BccI CCATC 7 cut(s) 100, 447, 570, 647, 923, 1096, 1163
BclI TGATCA 2 cut(s) 160, 1108
BcoDI GTCTC 2 cut(s) 267, 705
BfaI CTAG 5 cut(s) 51, 155, 324, 465, 471
BfmI CTRYAG 2 cut(s) 441, 695
BisI GCNGC 6 cut(s) 139, 282, 285, 390, 477, 921
BlsI GCNGC 6 cut(s) 140, 283, 286, 391, 478, 922
Bme18I GGWCC 1 cut(s) 722
BmgT120I GGNCC 2 cut(s) 722, 909
BmiI GGNNCC 1 cut(s) 910
BmsI GCATC 3 cut(s) 268, 717, 1042
BpiI GAAGAC 1 cut(s) 518
BpmI CTGGAG 1 cut(s) 924
Bpu10I CCTNAGC 1 cut(s) 833
BpuEI CTTGAG 1 cut(s) 563
Bsa29I ATCGAT 1 cut(s) 1071
BsaAI YACGTR 1 cut(s) 1114
BsaI GGTCTC 1 cut(s) 267
BsaJI CCNNGG 4 cut(s) 257, 480, 501, 718
BsaXI ACNNNNNCTCC 2 cut(s) 353, 383
Bsc4I CCNNNNNNNGG 3 cut(s) 486, 945, 974
Bse1I ACTGG 2 cut(s) 94, 641
Bse21I CCTNAGG 1 cut(s) 954
BseCI ATCGAT 1 cut(s) 1071
BseDI CCNNGG 4 cut(s) 257, 480, 501, 718
BseGI GGATG 2 cut(s) 14, 649
BseLI CCNNNNNNNGG 3 cut(s) 486, 945, 974
BseMII CTCAG 3 cut(s) 544, 847, 945
BseNI ACTGG 2 cut(s) 94, 641
BseRI GAGGAG 1 cut(s) 1043
BseSI GKGCMC 1 cut(s) 683
BseXI GCAGC 6 cut(s) 150, 271, 293, 376, 463, 932
BsgI GTGCAG 2 cut(s) 183, 317
Bsh1236I CGCG 1 cut(s) 259
BshFI GGCC 2 cut(s) 911, 967
BshVI ATCGAT 1 cut(s) 1071
BsiHKAI GWGCWC 1 cut(s) 407
BslFI GGGAC 1 cut(s) 190
BslI CCNNNNNNNGG 3 cut(s) 486, 945, 974
BsmAI GTCTC 2 cut(s) 267, 705
BsmFI GGGAC 1 cut(s) 190
BsnI GGCC 2 cut(s) 911, 967
Bso31I GGTCTC 1 cut(s) 267
Bsp1286I GDGCHC 3 cut(s) 8, 407, 683
Bsp1407I TGTACA 1 cut(s) 774
Bsp143I GATC 5 cut(s) 160, 553, 691, 822, 1108
BspACI CCGC 3 cut(s) 257, 259, 975
BspANI GGCC 2 cut(s) 911, 967
BspCNI CTCAG 3 cut(s) 543, 846, 946
BspDI ATCGAT 1 cut(s) 1071
BspFNI CGCG 1 cut(s) 259
BspLI GGNNCC 1 cut(s) 910
BspMAI CTGCAG 2 cut(s) 445, 699
BspPI GGATC 2 cut(s) 548, 830
BspTNI GGTCTC 1 cut(s) 267
BsrGI TGTACA 1 cut(s) 774
BsrI ACTGG 2 cut(s) 94, 641
BssECI CCNNGG 4 cut(s) 257, 480, 501, 718
BssMI GATC 5 cut(s) 160, 553, 691, 822, 1108
BssSI CACGAG 1 cut(s) 804
BssT1I CCWWGG 2 cut(s) 480, 718
Bst2BI CACGAG 1 cut(s) 804
BstAUI TGTACA 1 cut(s) 774
BstBAI YACGTR 1 cut(s) 1114
BstC8I GCNNGC 2 cut(s) 8, 375
BstDEI CTNAG 5 cut(s) 395, 530, 816, 833, 954
BstDSI CCRYGG 2 cut(s) 257, 501
BstF5I GGATG 2 cut(s) 14, 649
BstFNI CGCG 1 cut(s) 259
BstHHI GCGC 1 cut(s) 347
BstKTI GATC 5 cut(s) 163, 556, 694, 825, 1111
BstMAI GTCTC 2 cut(s) 267, 705
BstMBI GATC 5 cut(s) 160, 553, 691, 822, 1108
BstMWI GCNNNNNNNGC 4 cut(s) 344, 370, 908, 917
BstSFI CTRYAG 2 cut(s) 441, 695
BstSLI GKGCMC 1 cut(s) 683
BstUI CGCG 1 cut(s) 259
BstV1I GCAGC 6 cut(s) 150, 271, 293, 376, 463, 932
BstV2I GAAGAC 1 cut(s) 518
BstX2I RGATCY 1 cut(s) 822
BstXI CCANNNNNNTGG 2 cut(s) 246, 569
BstYI RGATCY 1 cut(s) 822
Bsu15I ATCGAT 1 cut(s) 1071
Bsu36I CCTNAGG 1 cut(s) 954
BsuRI GGCC 2 cut(s) 911, 967
BsuTUI ATCGAT 1 cut(s) 1071
BtgI CCRYGG 2 cut(s) 257, 501
BtgZI GCGATG 1 cut(s) 901
BtsCI GGATG 2 cut(s) 14, 649
Cac8I GCNNGC 2 cut(s) 8, 375
CaiI CAGNNNCTG 1 cut(s) 440
CfoI GCGC 1 cut(s) 347
Cfr13I GGNCC 2 cut(s) 722, 909
Cfr42I CCGCGG 1 cut(s) 260
ClaI ATCGAT 1 cut(s) 1071
Csp6I GTAC 2 cut(s) 589, 775
CviQI GTAC 2 cut(s) 589, 775
DdeI CTNAG 5 cut(s) 395, 530, 816, 833, 954
DpnI GATC 5 cut(s) 162, 555, 693, 824, 1110
DpnII GATC 5 cut(s) 160, 553, 691, 822, 1108
Ecl136II GAGCTC 1 cut(s) 405
Eco130I CCWWGG 2 cut(s) 480, 718
Eco24I GRGCYC 2 cut(s) 8, 407
Eco31I GGTCTC 1 cut(s) 267
Eco32I GATATC 1 cut(s) 1150
Eco47I GGWCC 1 cut(s) 722
Eco53kI GAGCTC 1 cut(s) 405
Eco72I CACGTG 1 cut(s) 1114
Eco81I CCTNAGG 1 cut(s) 954
EcoICRI GAGCTC 1 cut(s) 405
EcoRI GAATTC 2 cut(s) 28, 1010
EcoRV GATATC 1 cut(s) 1150
EcoT14I CCWWGG 2 cut(s) 480, 718
EcoT22I ATGCAT 1 cut(s) 710
EcoT38I GRGCYC 2 cut(s) 8, 407
ErhI CCWWGG 2 cut(s) 480, 718
FalI AAGNNNNNCTT 6 cut(s) 654, 686, 809, 841, 860, 892
FaqI GGGAC 1 cut(s) 190
FauNDI CATATG 1 cut(s) 1093
FbaI TGATCA 2 cut(s) 160, 1108
Fnu4HI GCNGC 6 cut(s) 139, 282, 285, 390, 477, 921
FokI GGATG 1 cut(s) 656
FriOI GRGCYC 2 cut(s) 8, 407
Fsp4HI GCNGC 6 cut(s) 139, 282, 285, 390, 477, 921
FspBI CTAG 5 cut(s) 51, 155, 324, 465, 471
GlaI GCGC 1 cut(s) 346
GluI GCNGC 6 cut(s) 139, 282, 285, 390, 477, 921
GsuI CTGGAG 1 cut(s) 924
HaeIII GGCC 2 cut(s) 911, 967
HhaI GCGC 1 cut(s) 347
Hin6I GCGC 1 cut(s) 345
HinP1I GCGC 1 cut(s) 345
HindIII AAGCTT 2 cut(s) 738, 767
HinfI GANTC 3 cut(s) 128, 422, 514
HphI GGTGA 2 cut(s) 170, 662
Hpy166II GTNNAC 5 cut(s) 307, 501, 667, 775, 1042
Hpy188I TCNGA 4 cut(s) 100, 436, 519, 533
Hpy188III TCNNGA 4 cut(s) 67, 450, 806, 953
Hpy8I GTNNAC 5 cut(s) 307, 501, 667, 775, 1042
HpyAV CCTTC 2 cut(s) 34, 484
HpyCH4IV ACGT 1 cut(s) 1113
HpyCH4V TGCA 9 cut(s) 200, 281, 287, 298, 338, 443, 583, 697, 708
HpyF10VI GCNNNNNNNGC 4 cut(s) 344, 370, 908, 917
HpyF3I CTNAG 5 cut(s) 395, 530, 816, 833, 954
HpySE526I ACGT 1 cut(s) 1113
HspAI GCGC 1 cut(s) 345
Ksp22I TGATCA 2 cut(s) 160, 1108
KspI CCGCGG 1 cut(s) 260
Kzo9I GATC 5 cut(s) 160, 553, 691, 822, 1108
LmnI GCTCC 5 cut(s) 361, 402, 410, 615, 1030
Lsp1109I GCAGC 6 cut(s) 150, 271, 293, 376, 463, 932
LweI GCATC 3 cut(s) 268, 717, 1042
MaeI CTAG 5 cut(s) 51, 155, 324, 465, 471
MaeII ACGT 1 cut(s) 1113
MaeIII GTNAC 1 cut(s) 314
MalI GATC 5 cut(s) 162, 555, 693, 824, 1110
MboI GATC 5 cut(s) 160, 553, 691, 822, 1108
MboII GAAGA 7 cut(s) 137, 254, 523, 622, 877, 880, 1157
MfeI CAATTG 1 cut(s) 267
MflI RGATCY 1 cut(s) 822
MhlI GDGCHC 3 cut(s) 8, 407, 683
MluCI AATT 8 cut(s) 28, 57, 216, 267, 521, 577, 1000, 1010
MlyI GAGTC 1 cut(s) 508
MmeI TCCRAC 1 cut(s) 621
Mph1103I ATGCAT 1 cut(s) 710
MseI TTAA 4 cut(s) 80, 233, 429, 606
MslI CAYNNNNRTG 2 cut(s) 23, 567
MspA1I CMGCKG 4 cut(s) 166, 259, 284, 975
MunI CAATTG 1 cut(s) 267
MvnI CGCG 1 cut(s) 259
MwoI GCNNNNNNNGC 4 cut(s) 344, 370, 908, 917
NdeI CATATG 1 cut(s) 1093
NdeII GATC 5 cut(s) 160, 553, 691, 822, 1108
NlaIV GGNNCC 1 cut(s) 910
NsiI ATGCAT 1 cut(s) 710
PfeI GAWTC 2 cut(s) 128, 422
PkrI GCNGC 6 cut(s) 140, 283, 286, 391, 478, 922
PleI GAGTC 1 cut(s) 508
PmaCI CACGTG 1 cut(s) 1114
PmlI CACGTG 1 cut(s) 1114
PpsI GAGTC 1 cut(s) 508
Ppu21I YACGTR 1 cut(s) 1114
Psp124BI GAGCTC 1 cut(s) 407
PspCI CACGTG 1 cut(s) 1114
PspN4I GGNNCC 1 cut(s) 910
PspPI GGNCC 2 cut(s) 722, 909
PstI CTGCAG 2 cut(s) 445, 699
PstNI CAGNNNCTG 1 cut(s) 440
PsuI RGATCY 1 cut(s) 822
PvuII CAGCTG 2 cut(s) 166, 284
RsaI GTAC 2 cut(s) 590, 776
RsaNI GTAC 2 cut(s) 589, 775
RseI CAYNNNNRTG 2 cut(s) 23, 567
SacI GAGCTC 1 cut(s) 407
SacII CCGCGG 1 cut(s) 260
SaqAI TTAA 4 cut(s) 80, 233, 429, 606
SatI GCNGC 6 cut(s) 139, 282, 285, 390, 477, 921
Sau3AI GATC 5 cut(s) 160, 553, 691, 822, 1108
Sau96I GGNCC 2 cut(s) 722, 909
SchI GAGTC 1 cut(s) 508
SduI GDGCHC 3 cut(s) 8, 407, 683
SfaNI GCATC 3 cut(s) 268, 717, 1042
SfcI CTRYAG 2 cut(s) 441, 695
Sfr303I CCGCGG 1 cut(s) 260
SgrBI CCGCGG 1 cut(s) 260
SinI GGWCC 1 cut(s) 722
SmiMI CAYNNNNRTG 2 cut(s) 23, 567
SmlI CTYRAG 1 cut(s) 542
SmoI CTYRAG 1 cut(s) 542
Sse9I AATT 8 cut(s) 28, 57, 216, 267, 521, 577, 1000, 1010
SsiI CCGC 3 cut(s) 257, 259, 975
SspI AATATT 1 cut(s) 172
SspMI CTAG 5 cut(s) 51, 155, 324, 465, 471
SstI GAGCTC 1 cut(s) 407
StyI CCWWGG 2 cut(s) 480, 718
TaiI ACGT 1 cut(s) 1116
TaqI TCGA 1 cut(s) 1071
TasI AATT 8 cut(s) 28, 57, 216, 267, 521, 577, 1000, 1010
TatI WGTACW 2 cut(s) 588, 774
TfiI GAWTC 2 cut(s) 128, 422
Tru1I TTAA 4 cut(s) 80, 233, 429, 606
Tru9I TTAA 4 cut(s) 80, 233, 429, 606
TseI GCWGC 6 cut(s) 138, 281, 284, 389, 476, 920
TspDTI ATGAA 4 cut(s) 41, 211, 759, 1003
TspGWI ACGGA 3 cut(s) 397, 415, 518
VpaK11BI GGWCC 1 cut(s) 722
XapI RAATTY 5 cut(s) 28, 216, 577, 1000, 1010
XspI CTAG 5 cut(s) 51, 155, 324, 465, 471
Zsp2I ATGCAT 1 cut(s) 710
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.