pycom11g00560

calmodulin-binding family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0000760
Physical Location & Seq
Reverse (-)
410753 .. 412515
1763 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g00560.2

Sequence Viewer

Length: 1002 bp
ATGGGAATTTCCTTTTCTTGCCCATTTTCGCAGTACAGTGATTTGGAAAGTGGGTTGGATTCGATAATTGTGAAGTCCATAAGCTTTGGAGACAATGAAGATAAAACTCCGGCCAGATCAGTTAGTTTCAATGGTGGTGATGCAGAACCCACAATAATGAAATCCTTGGGTTCCGGAAGGATGACACTTGAAACTTCTGTTAGCTTTAAAGGCAGAGACTTGGAGAAAATGATGTTGCTCAAGGTTCCTTCGTTAGAAAAGAGCATGACCGTTGCATCAGTCAGCCCTCTCAGCAAAGAAATGAACGTTCCCTCCCCAAGGTCAGATAGTCCTCTGGTGATGATCCGGCCCTTGCAACTTTTGGATCCCGCCAGCCCCAAACACATGGCCGCAATTAAATTGCAGAAAGTATACAAAAGTTTTCGAACCAGACGAAAGTTAGCAGATTGTGCAGTTCTTGTTGAACAGAGCTGGTGGAAGCTGTTAGATTTTGCTGAGCTCAAGCGGAGTTCTATATCATTCTTTGAAATTGAGAAAAATGAAACTGCCATGTCGCGTTGGTCTAGAGCAAGGACCAGAGCCGCCAAGGTTGGGAAAGGTCTATCGAAGAATGAAAAGGCACAAAAGCTTGCTTTGCAGCATTGGCTTGAGGCGATGGAAAGAATGGCCTATGAAGTTGTTGTGGAGGATGGAAAATTCTTCTACAAACAATCAGGGGAGCTTCTCCATACAACCGGAGAATCCGATGATACCAAATGGATTTTCGTTCTTAGTGCTTCTAGATCCTTATACGTTGGCAAGAAAAAGAAGGGGACGTTTCAACATTCAAGTTTTTTGGCTGGAGGAGCCACATCTGCTGCCGGGAGATTAGTTGTTGAAAACGGCATCCTTAAGGCAGTTTGGCCTCACAGTGGTCACTATCGGCCTACAGAAGAAAATTTCAGAGACTTTGTCTCGTTCCTTGAAGAGAACAATGTGGATCGATCAAAGATGTTAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

334

Amino Acids

37.29

Weight (kDa)

9.42

Isoelectric Point (pI)

47.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 411
AccII CGCG 1 cut(s) 556
AccIII TCCGGA 1 cut(s) 173
AciI CCGC 4 cut(s) 369, 390, 505, 582
AclI AACGTT 1 cut(s) 306
AclWI GGATC 5 cut(s) 337, 359, 372, 777, 987
AcoI YGGCCR 2 cut(s) 111, 387
AcsI RAATTY 3 cut(s) 6, 695, 937
AfaI GTAC 1 cut(s) 35
AfiI CCNNNNNNNGG 3 cut(s) 318, 591, 911
AflII CTTAAG 1 cut(s) 890
AgsI TTSAA 8 cut(s) 130, 191, 464, 527, 821, 828, 878, 965
AjuI GAANNNNNNNTTGG 2 cut(s) 746, 778
AloI GAACNNNNNNTCC 2 cut(s) 296, 328
AluBI AGCT 7 cut(s) 84, 204, 471, 481, 499, 628, 721
AluI AGCT 7 cut(s) 84, 204, 471, 481, 499, 628, 721
Alw21I GWGCWC 1 cut(s) 501
Alw26I GTCTC 4 cut(s) 84, 210, 939, 958
AlwI GGATC 5 cut(s) 337, 359, 372, 777, 987
Aor13HI TCCGGA 1 cut(s) 173
AoxI GGCC 6 cut(s) 111, 347, 387, 666, 902, 923
ApeKI GCWGC 2 cut(s) 637, 857
ApoI RAATTY 3 cut(s) 6, 695, 937
AspS9I GGNCC 2 cut(s) 348, 573
AsuC2I CCSGG 1 cut(s) 862
AsuHPI GGTGA 2 cut(s) 149, 349
AsuII TTCGAA 1 cut(s) 424
AvaII GGWCC 1 cut(s) 573
BamHI GGATCC 1 cut(s) 364
BanII GRGCYC 1 cut(s) 501
Bbv12I GWGCWC 1 cut(s) 501
BbvI GCAGC 2 cut(s) 649, 844
BccI CCATC 2 cut(s) 649, 683
BceAI ACGGC 1 cut(s) 898
BcnI CCSGG 1 cut(s) 862
BcoDI GTCTC 4 cut(s) 84, 210, 939, 958
BfaI CTAG 2 cut(s) 564, 780
BfmI CTRYAG 1 cut(s) 927
BfrI CTTAAG 1 cut(s) 890
BisI GCNGC 4 cut(s) 390, 582, 638, 858
BlpI GCTNAGC 1 cut(s) 495
BlsI GCNGC 4 cut(s) 391, 583, 639, 859
Bme1390I CCNGG 1 cut(s) 862
Bme18I GGWCC 1 cut(s) 573
BmgT120I GGNCC 2 cut(s) 348, 573
BmiI GGNNCC 4 cut(s) 172, 246, 366, 847
BmrFI CCNGG 1 cut(s) 862
BmsI GCATC 3 cut(s) 130, 284, 894
BpmI CTGGAG 1 cut(s) 861
Bpu1102I GCTNAGC 1 cut(s) 495
Bpu14I TTCGAA 1 cut(s) 424
BpuEI CTTGAG 3 cut(s) 224, 485, 668
BpuMI CCSGG 1 cut(s) 862
Bsa29I ATCGAT 1 cut(s) 982
BsaJI CCNNGG 3 cut(s) 165, 317, 585
BsaWI WCCGGW 2 cut(s) 173, 734
BsaXI ACNNNNNCTCC 2 cut(s) 296, 326
Bsc4I CCNNNNNNNGG 3 cut(s) 318, 591, 911
BseAI TCCGGA 1 cut(s) 173
BseCI ATCGAT 1 cut(s) 982
BseDI CCNNGG 3 cut(s) 165, 317, 585
BseGI GGATG 3 cut(s) 186, 694, 885
BseLI CCNNNNNNNGG 3 cut(s) 318, 591, 911
BseMII CTCAG 2 cut(s) 304, 486
BseRI GAGGAG 1 cut(s) 858
BseXI GCAGC 2 cut(s) 649, 844
BsgI GTGCAG 1 cut(s) 471
Bsh1236I CGCG 1 cut(s) 556
BshFI GGCC 6 cut(s) 113, 349, 389, 668, 904, 925
BshVI ATCGAT 1 cut(s) 982
BsiHKAI GWGCWC 1 cut(s) 501
BsiSI CCGG 5 cut(s) 110, 174, 346, 735, 861
BslFI GGGAC 1 cut(s) 826
BslI CCNNNNNNNGG 3 cut(s) 318, 591, 911
BsmAI GTCTC 4 cut(s) 84, 210, 939, 958
BsmFI GGGAC 1 cut(s) 826
BsnI GGCC 6 cut(s) 113, 349, 389, 668, 904, 925
Bsp119I TTCGAA 1 cut(s) 424
Bsp1286I GDGCHC 1 cut(s) 501
Bsp13I TCCGGA 1 cut(s) 173
Bsp143I GATC 6 cut(s) 116, 342, 364, 782, 979, 983
Bsp1720I GCTNAGC 1 cut(s) 495
BspACI CCGC 4 cut(s) 369, 390, 505, 582
BspANI GGCC 6 cut(s) 113, 349, 389, 668, 904, 925
BspCNI CTCAG 2 cut(s) 303, 487
BspDI ATCGAT 1 cut(s) 982
BspEI TCCGGA 1 cut(s) 173
BspFNI CGCG 1 cut(s) 556
BspLI GGNNCC 4 cut(s) 172, 246, 366, 847
BspPI GGATC 5 cut(s) 337, 359, 372, 777, 987
BspT104I TTCGAA 1 cut(s) 424
BspTI CTTAAG 1 cut(s) 890
BssECI CCNNGG 3 cut(s) 165, 317, 585
BssMI GATC 6 cut(s) 116, 342, 364, 782, 979, 983
BssNAI GTATAC 1 cut(s) 412
BssT1I CCWWGG 3 cut(s) 165, 317, 585
Bst1107I GTATAC 1 cut(s) 412
Bst4CI ACNGT 3 cut(s) 38, 271, 911
Bst6I CTCTTC 1 cut(s) 960
BstAFI CTTAAG 1 cut(s) 890
BstAPI GCANNNNNTGC 1 cut(s) 449
BstBI TTCGAA 1 cut(s) 424
BstC8I GCNNGC 2 cut(s) 373, 630
BstDEI CTNAG 3 cut(s) 290, 495, 770
BstENI CCTNNNNNAGG 1 cut(s) 316
BstF5I GGATG 3 cut(s) 186, 694, 885
BstFNI CGCG 1 cut(s) 556
BstKTI GATC 6 cut(s) 119, 345, 367, 785, 982, 986
BstMAI GTCTC 4 cut(s) 84, 210, 939, 958
BstMBI GATC 6 cut(s) 116, 342, 364, 782, 979, 983
BstMWI GCNNNNNNNGC 7 cut(s) 210, 291, 449, 634, 643, 845, 854
BstSCI CCNGG 1 cut(s) 860
BstSFI CTRYAG 1 cut(s) 927
BstUI CGCG 1 cut(s) 556
BstV1I GCAGC 2 cut(s) 649, 844
BstX2I RGATCY 2 cut(s) 364, 782
BstXI CCANNNNNNTGG 1 cut(s) 385
BstYI RGATCY 2 cut(s) 364, 782
BstZ17I GTATAC 1 cut(s) 412
Bsu15I ATCGAT 1 cut(s) 982
BsuRI GGCC 6 cut(s) 113, 349, 389, 668, 904, 925
BsuTUI ATCGAT 1 cut(s) 982
BtgZI GCGATG 1 cut(s) 668
BtsCI GGATG 3 cut(s) 186, 694, 885
BtsIMutI CAGTG 2 cut(s) 43, 916
Cac8I GCNNGC 2 cut(s) 373, 630
Cfr13I GGNCC 2 cut(s) 348, 573
ClaI ATCGAT 1 cut(s) 982
Csp6I GTAC 1 cut(s) 34
CviAII CATG 3 cut(s) 265, 385, 550
CviQI GTAC 1 cut(s) 34
DdeI CTNAG 3 cut(s) 290, 495, 770
DpnI GATC 6 cut(s) 118, 344, 366, 784, 981, 985
DpnII GATC 6 cut(s) 116, 342, 364, 782, 979, 983
DraI TTTAAA 1 cut(s) 208
EaeI YGGCCR 2 cut(s) 111, 387
Eam1104I CTCTTC 1 cut(s) 960
EarI CTCTTC 1 cut(s) 960
Ecl136II GAGCTC 1 cut(s) 499
Eco130I CCWWGG 3 cut(s) 165, 317, 585
Eco24I GRGCYC 1 cut(s) 501
Eco47I GGWCC 1 cut(s) 573
Eco53kI GAGCTC 1 cut(s) 499
EcoICRI GAGCTC 1 cut(s) 499
EcoNI CCTNNNNNAGG 1 cut(s) 316
EcoT14I CCWWGG 3 cut(s) 165, 317, 585
EcoT38I GRGCYC 1 cut(s) 501
ErhI CCWWGG 3 cut(s) 165, 317, 585
FaeI CATG 3 cut(s) 268, 388, 553
FaiI YATR 9 cut(s) 80, 266, 386, 412, 515, 551, 672, 729, 790
FaqI GGGAC 1 cut(s) 826
FatI CATG 3 cut(s) 264, 384, 549
FauI CCCGC 1 cut(s) 376
FblI GTMKAC 1 cut(s) 411
Fnu4HI GCNGC 4 cut(s) 390, 582, 638, 858
FokI GGATG 3 cut(s) 193, 701, 872
FriOI GRGCYC 1 cut(s) 501
Fsp4HI GCNGC 4 cut(s) 390, 582, 638, 858
FspBI CTAG 2 cut(s) 564, 780
GluI GCNGC 4 cut(s) 390, 582, 638, 858
GsuI CTGGAG 1 cut(s) 861
HaeIII GGCC 6 cut(s) 113, 349, 389, 668, 904, 925
HapII CCGG 5 cut(s) 110, 174, 346, 735, 861
Hin1II CATG 3 cut(s) 268, 388, 553
HindIII AAGCTT 2 cut(s) 82, 626
HinfI GANTC 2 cut(s) 59, 740
HpaII CCGG 5 cut(s) 110, 174, 346, 735, 861
HphI GGTGA 2 cut(s) 149, 349
Hpy166II GTNNAC 1 cut(s) 412
Hpy188I TCNGA 3 cut(s) 325, 745, 944
Hpy188III TCNNGA 3 cut(s) 174, 564, 780
Hpy8I GTNNAC 1 cut(s) 412
HpyAV CCTTC 3 cut(s) 171, 258, 802
HpyCH4III ACNGT 3 cut(s) 38, 271, 911
HpyCH4IV ACGT 3 cut(s) 306, 792, 815
HpyCH4V TGCA 6 cut(s) 143, 275, 355, 403, 452, 637
HpyF10VI GCNNNNNNNGC 7 cut(s) 210, 291, 449, 634, 643, 845, 854
HpyF3I CTNAG 3 cut(s) 290, 495, 770
HpySE526I ACGT 3 cut(s) 306, 792, 815
Hsp92II CATG 3 cut(s) 268, 388, 553
Kpn2I TCCGGA 1 cut(s) 173
Kzo9I GATC 6 cut(s) 116, 342, 364, 782, 979, 983
LmnI GCTCC 2 cut(s) 718, 845
Lsp1109I GCAGC 2 cut(s) 649, 844
LweI GCATC 3 cut(s) 130, 284, 894
MaeI CTAG 2 cut(s) 564, 780
MaeII ACGT 3 cut(s) 306, 792, 815
MaeIII GTNAC 1 cut(s) 914
MalI GATC 6 cut(s) 118, 344, 366, 784, 981, 985
MboI GATC 6 cut(s) 116, 342, 364, 782, 979, 983
MboII GAAGA 5 cut(s) 110, 619, 691, 944, 977
MflI RGATCY 2 cut(s) 364, 782
MhlI GDGCHC 1 cut(s) 501
MluCI AATT 7 cut(s) 6, 66, 393, 398, 528, 695, 937
MmeI TCCRAC 1 cut(s) 36
MnlI CCTC 7 cut(s) 297, 322, 342, 643, 679, 836, 915
MroI TCCGGA 1 cut(s) 173
MseI TTAA 5 cut(s) 207, 396, 891, 995, 1000
MslI CAYNNNNRTG 1 cut(s) 155
MspCI CTTAAG 1 cut(s) 890
MspI CCGG 5 cut(s) 110, 174, 346, 735, 861
MspR9I CCNGG 1 cut(s) 862
MvnI CGCG 1 cut(s) 556
MwoI GCNNNNNNNGC 7 cut(s) 210, 291, 449, 634, 643, 845, 854
NciI CCSGG 1 cut(s) 862
NdeII GATC 6 cut(s) 116, 342, 364, 782, 979, 983
NlaIII CATG 3 cut(s) 268, 388, 553
NlaIV GGNNCC 4 cut(s) 172, 246, 366, 847
NmuCI GTSAC 1 cut(s) 914
NspV TTCGAA 1 cut(s) 424
PcsI WCGNNNNNNNCGW 1 cut(s) 430
PfeI GAWTC 2 cut(s) 59, 740
PflFI GACNNNGTC 1 cut(s) 950
PkrI GCNGC 4 cut(s) 391, 583, 639, 859
Psp124BI GAGCTC 1 cut(s) 501
Psp1406I AACGTT 1 cut(s) 306
PspN4I GGNNCC 4 cut(s) 172, 246, 366, 847
PspPI GGNCC 2 cut(s) 348, 573
PsuI RGATCY 2 cut(s) 364, 782
PsyI GACNNNGTC 1 cut(s) 950
RsaI GTAC 1 cut(s) 35
RsaNI GTAC 1 cut(s) 34
RseI CAYNNNNRTG 1 cut(s) 155
SacI GAGCTC 1 cut(s) 501
SaqAI TTAA 5 cut(s) 207, 396, 891, 995, 1000
SatI GCNGC 4 cut(s) 390, 582, 638, 858
Sau3AI GATC 6 cut(s) 116, 342, 364, 782, 979, 983
Sau96I GGNCC 2 cut(s) 348, 573
ScrFI CCNGG 1 cut(s) 862
SduI GDGCHC 1 cut(s) 501
SfaNI GCATC 3 cut(s) 130, 284, 894
SfcI CTRYAG 1 cut(s) 927
SfuI TTCGAA 1 cut(s) 424
SinI GGWCC 1 cut(s) 573
SmiMI CAYNNNNRTG 1 cut(s) 155
SmlI CTYRAG 4 cut(s) 239, 500, 647, 890
SmoI CTYRAG 4 cut(s) 239, 500, 647, 890
Sse9I AATT 7 cut(s) 6, 66, 393, 398, 528, 695, 937
SsiI CCGC 4 cut(s) 369, 390, 505, 582
SspMI CTAG 2 cut(s) 564, 780
SstI GAGCTC 1 cut(s) 501
StyD4I CCNGG 1 cut(s) 860
StyI CCWWGG 3 cut(s) 165, 317, 585
TaaI ACNGT 3 cut(s) 38, 271, 911
TaiI ACGT 3 cut(s) 309, 795, 818
TaqI TCGA 4 cut(s) 62, 424, 605, 982
TasI AATT 7 cut(s) 6, 66, 393, 398, 528, 695, 937
TatI WGTACW 1 cut(s) 33
TauI GCSGC 2 cut(s) 392, 584
TfiI GAWTC 2 cut(s) 59, 740
Tru1I TTAA 5 cut(s) 207, 396, 891, 995, 1000
Tru9I TTAA 5 cut(s) 207, 396, 891, 995, 1000
TscAI CASTG 2 cut(s) 43, 916
TseFI GTSAC 1 cut(s) 914
TseI GCWGC 2 cut(s) 637, 857
Tsp45I GTSAC 1 cut(s) 914
TspDTI ATGAA 6 cut(s) 111, 173, 317, 555, 627, 687
TspRI CASTG 2 cut(s) 43, 916
Tth111I GACNNNGTC 1 cut(s) 950
Vha464I CTTAAG 1 cut(s) 890
VpaK11BI GGWCC 1 cut(s) 573
XagI CCTNNNNNAGG 1 cut(s) 316
XapI RAATTY 3 cut(s) 6, 695, 937
XbaI TCTAGA 2 cut(s) 563, 779
XmiI GTMKAC 1 cut(s) 411
XspI CTAG 2 cut(s) 564, 780
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.