Rw2G035620

Short calmodulin-binding motif containing conserved Ile and Gln residues.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000760
Physical Location & Seq
Reverse (-)
58665409 .. 58667914
2506 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G035620.1

Sequence Viewer

Length: 1557 bp
ATGGAGATAATGAGATTCACTACCTTGAATGTGGATTTTCTTATGATACAAACACATATTTCTGAGGCACATGCATTTTGTTTTGACAGAACTTGTAGAATGGCGACAATGACATCTATCAGTTTAGTGTCCAAGAGAACGAAGGTGATGTCAAGAACAGTTAGTTTTGAGAACCCCGATTCAAGCAACACCAACAGATCCTATGGCCTAGATACATTGGTTTTTGAAAAATCTGTATGCTACAAGAAAAGGAAAGCAGGACAACTAAAGCTCGATACTTCAACTTTGCTACCCAAGCTAGAAGGTTGTGTTTCTCCACAGACCCCACGCGAGCTTGATGAAGCTGCAATCAAGCTGCAGAAATTTTACAAGAGTTACCGAACTAGACGAAACCTTGCAGATTGTGCTGTTGTTGTCGAGGAGCTATGGTGGAAGGCATTAGACTTTGCTGCACTTAGGCGGAGCTCTGTCTCATTCTTTGAATCTGTTGAATCAGAAACTGCAGTTTCCAGATGGGCAAGGGCTAAGACTAGAGCTGCCAAGGTTGGGAAGGGTTTGTCCAAGGATGAGAAGGCTCAGAAATTAGCTTTAAGACATTGGCTTGAGGCTATTGATCCACGGCATCGATATGGACATAATCTGCACTTGTATTATGATGTCTGGTTTAATAGTGCGAGCTCTCAACCTTTCTTCTACTGGTTGGATGTTGGAGATGGTAAAGAAGAAAATCTTGAGAAGTGTCCAAGAGCGGATCTTCAACGCCAATGCATCAAGTACCTTGGACCAAATGAGAGGGAAATATATGAAGTGATAGTGGCTAGTGGGAAGCTTGTATACAGACAAAGTGGCAACCTTGTGACTACTACTGAGGGTTCCAAATGGATCTTTGTTCTCAGTGCATCGAGGATTATGTATGTTGCGGAAAAGAAGAAAGGCTATTTCCAGCACTCCAGTTTTCTATCCGGAGGTGCCACAATTGCAGCGGGGAGATTAGTTGCATCTGATGGGGTTCTTGAGGCTGTATGGTGCTACAGTGGTCATTATCGCCCGACAGAAGAGCACTTTTTGGAGTTCATTAGCTTCTTAGAGGAGCAACAGGTGGACTTGACAAATGTCAAAAAACATCCTATAGATGATGATATTCCACCTTCAGAATCTACCAGTAAAGATATGAGCACCCTTGAGGATATCCCATCGGCAAACATTGAGAAGAGTACTAATGAAAATGAGCGTGCCGTTGACGCTTCCATAGTAAGTGATGACAACCAAGACATTGTGGAAGCACCAAAATTTGACTCGGGATCAGGGGGATTGCCTTGCAAATGGACTACAGGAGCTGGCCCTCGAATCGGATGTGTGAGAGACTACCCCGCACGGCTGCAATTTCTGGCACTTGAGCAAGTCAATCTGTCACCAAGAATCAAACCAGAACGCACTGCAAGCAATAACACCCCAGTGCCTTCACCAAGACCTAGTCCCAAGTTCCACTTATCACCTAGACTTGCATATATGGGACTTCCTAGCCCCAGAAGATTGCATAATTTGACTCCTCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

518

Amino Acids

58.57

Weight (kDa)

8.72

Isoelectric Point (pI)

44.84

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 968
AccBSI CCGCTC 1 cut(s) 749
AccI GTMKAC 1 cut(s) 834
AccII CGCG 1 cut(s) 330
AccIII TCCGGA 1 cut(s) 962
AciI CCGC 5 cut(s) 460, 749, 920, 983, 1371
AclWI GGATC 5 cut(s) 192, 608, 759, 890, 1309
AcsI RAATTY 2 cut(s) 362, 1289
AcuI CTGAAG 1 cut(s) 1134
AfaI GTAC 2 cut(s) 776, 1216
AfiI CCNNNNNNNGG 2 cut(s) 546, 1349
AgsI TTSAA 7 cut(s) 28, 183, 227, 282, 482, 491, 758
AleI CACNNNNGTG 1 cut(s) 1454
Alw21I GWGCWC 4 cut(s) 467, 680, 1062, 1178
Alw26I GTCTC 2 cut(s) 475, 1356
AlwI GGATC 5 cut(s) 192, 608, 759, 890, 1309
AlwNI CAGNNNCTG 2 cut(s) 500, 1337
Ama87I CYCGRG 1 cut(s) 1297
Aor13HI TCCGGA 1 cut(s) 962
AoxI GGCC 2 cut(s) 205, 1339
ApeKI GCWGC 6 cut(s) 344, 355, 449, 536, 980, 1378
ApoI RAATTY 2 cut(s) 362, 1289
ArsI GACNNNNNNTTYG 2 cut(s) 134, 166
AspS9I GGNCC 2 cut(s) 782, 1340
AsuHPI GGTGA 4 cut(s) 157, 1404, 1455, 1485
AvaI CYCGRG 1 cut(s) 1297
AvaII GGWCC 1 cut(s) 782
BaeI ACNNNNGTAYC 2 cut(s) 204, 237
BanI GGYRCC 1 cut(s) 968
BanII GRGCYC 2 cut(s) 467, 680
BarI GAAGNNNNNNTAC 2 cut(s) 818, 850
Bbv12I GWGCWC 4 cut(s) 467, 680, 1062, 1178
BbvI GCAGC 6 cut(s) 331, 342, 436, 523, 992, 1365
BccI CCATC 4 cut(s) 507, 707, 998, 1201
BceAI ACGGC 3 cut(s) 635, 1220, 1391
BcoDI GTCTC 2 cut(s) 475, 1356
BfaI CTAG 8 cut(s) 209, 299, 384, 531, 819, 1473, 1497, 1521
BfmI CTRYAG 5 cut(s) 356, 501, 1030, 1128, 1329
BisI GCNGC 6 cut(s) 345, 356, 450, 537, 981, 1379
BlsI GCNGC 6 cut(s) 346, 357, 451, 538, 982, 1380
BmcAI AGTACT 1 cut(s) 1216
Bme18I GGWCC 1 cut(s) 782
BmeT110I CYCGRG 1 cut(s) 1297
BmgT120I GGNCC 2 cut(s) 782, 1340
BmiI GGNNCC 2 cut(s) 874, 970
BmrI ACTGGG 1 cut(s) 1448
BmsI GCATC 4 cut(s) 631, 777, 908, 1007
BmuI ACTGGG 1 cut(s) 1448
BoxI GACNNNNGTC 1 cut(s) 1112
BpmI CTGGAG 1 cut(s) 934
BpuEI CTTGAG 5 cut(s) 623, 752, 1034, 1202, 1415
Bsa29I ATCGAT 1 cut(s) 625
BsaJI CCNNGG 4 cut(s) 540, 561, 617, 778
BsaWI WCCGGW 1 cut(s) 962
BsaXI ACNNNNNCTCC 2 cut(s) 413, 443
Bsc4I CCNNNNNNNGG 2 cut(s) 546, 1349
Bse1I ACTGG 4 cut(s) 701, 951, 1161, 1454
BseAI TCCGGA 1 cut(s) 962
BseCI ATCGAT 1 cut(s) 625
BseDI CCNNGG 4 cut(s) 540, 561, 617, 778
BseGI GGATG 4 cut(s) 571, 709, 1123, 1358
BseLI CCNNNNNNNGG 2 cut(s) 546, 1349
BseMII CTCAG 4 cut(s) 54, 590, 858, 907
BseNI ACTGG 4 cut(s) 701, 951, 1161, 1454
BseRI GAGGAG 3 cut(s) 434, 1103, 1539
BseXI GCAGC 6 cut(s) 331, 342, 436, 523, 992, 1365
BsgI GTGCAG 2 cut(s) 435, 626
Bsh1236I CGCG 1 cut(s) 330
BshFI GGCC 2 cut(s) 207, 1341
BshNI GGYRCC 1 cut(s) 968
BshVI ATCGAT 1 cut(s) 625
BsiHKAI GWGCWC 4 cut(s) 467, 680, 1062, 1178
BsiHKCI CYCGRG 1 cut(s) 1297
BsiSI CCGG 1 cut(s) 963
BslFI GGGAC 2 cut(s) 1461, 1527
BslI CCNNNNNNNGG 2 cut(s) 546, 1349
BsmAI GTCTC 2 cut(s) 475, 1356
BsmFI GGGAC 2 cut(s) 1461, 1527
BsnI GGCC 2 cut(s) 207, 1341
BsoBI CYCGRG 1 cut(s) 1297
Bsp1286I GDGCHC 4 cut(s) 467, 680, 1062, 1178
Bsp13I TCCGGA 1 cut(s) 962
Bsp143I GATC 5 cut(s) 197, 613, 751, 882, 1301
BspACI CCGC 5 cut(s) 460, 749, 920, 983, 1371
BspANI GGCC 2 cut(s) 207, 1341
BspCNI CTCAG 4 cut(s) 55, 589, 859, 906
BspDI ATCGAT 1 cut(s) 625
BspEI TCCGGA 1 cut(s) 962
BspFNI CGCG 1 cut(s) 330
BspLI GGNNCC 2 cut(s) 874, 970
BspMAI CTGCAG 2 cut(s) 360, 505
BspPI GGATC 5 cut(s) 192, 608, 759, 890, 1309
BspQI GCTCTTC 1 cut(s) 1050
BspT107I GGYRCC 1 cut(s) 968
BsrBI CCGCTC 1 cut(s) 749
BsrI ACTGG 4 cut(s) 701, 951, 1161, 1454
BssECI CCNNGG 4 cut(s) 540, 561, 617, 778
BssMI GATC 5 cut(s) 197, 613, 751, 882, 1301
BssNAI GTATAC 1 cut(s) 835
BssT1I CCWWGG 3 cut(s) 540, 561, 778
Bst1107I GTATAC 1 cut(s) 835
Bst4CI ACNGT 2 cut(s) 160, 1034
Bst6I CTCTTC 2 cut(s) 1050, 1205
BstAPI GCANNNNNTGC 1 cut(s) 404
BstC8I GCNNGC 5 cut(s) 332, 676, 1233, 1339, 1441
BstDEI CTNAG 8 cut(s) 63, 455, 525, 576, 867, 893, 1084, 1554
BstDSI CCRYGG 1 cut(s) 617
BstF5I GGATG 4 cut(s) 571, 709, 1123, 1358
BstFNI CGCG 1 cut(s) 330
BstKTI GATC 5 cut(s) 200, 616, 754, 885, 1304
BstMAI GTCTC 2 cut(s) 475, 1356
BstMBI GATC 5 cut(s) 197, 613, 751, 882, 1301
BstMWI GCNNNNNNNGC 5 cut(s) 295, 404, 977, 1241, 1440
BstNSI RCATGY 1 cut(s) 74
BstPAI GACNNNNGTC 1 cut(s) 1112
BstSFI CTRYAG 5 cut(s) 356, 501, 1030, 1128, 1329
BstUI CGCG 1 cut(s) 330
BstV1I GCAGC 6 cut(s) 331, 342, 436, 523, 992, 1365
BstX2I RGATCY 3 cut(s) 197, 751, 882
BstYI RGATCY 3 cut(s) 197, 751, 882
BstZ17I GTATAC 1 cut(s) 835
Bsu15I ATCGAT 1 cut(s) 625
BsuRI GGCC 2 cut(s) 207, 1341
BsuTUI ATCGAT 1 cut(s) 625
BtgI CCRYGG 1 cut(s) 617
BtsCI GGATG 4 cut(s) 571, 709, 1123, 1358
BtsI GCAGTG 1 cut(s) 1434
BtsIMutI CAGTG 4 cut(s) 901, 1039, 1434, 1461
Cac8I GCNNGC 5 cut(s) 332, 676, 1233, 1339, 1441
CaiI CAGNNNCTG 2 cut(s) 500, 1337
Cfr13I GGNCC 2 cut(s) 782, 1340
ClaI ATCGAT 1 cut(s) 625
CseI GACGC 1 cut(s) 1250
Csp6I GTAC 2 cut(s) 775, 1215
CviAII CATG 1 cut(s) 71
CviQI GTAC 2 cut(s) 775, 1215
DdeI CTNAG 8 cut(s) 63, 455, 525, 576, 867, 893, 1084, 1554
DpnI GATC 5 cut(s) 199, 615, 753, 884, 1303
DpnII GATC 5 cut(s) 197, 613, 751, 882, 1301
Eam1104I CTCTTC 2 cut(s) 1050, 1205
EarI CTCTTC 2 cut(s) 1050, 1205
EciI GGCGGA 1 cut(s) 475
Ecl136II GAGCTC 2 cut(s) 465, 678
Eco130I CCWWGG 3 cut(s) 540, 561, 778
Eco24I GRGCYC 2 cut(s) 467, 680
Eco32I GATATC 1 cut(s) 1189
Eco47I GGWCC 1 cut(s) 782
Eco53kI GAGCTC 2 cut(s) 465, 678
Eco57I CTGAAG 1 cut(s) 1134
Eco88I CYCGRG 1 cut(s) 1297
EcoICRI GAGCTC 2 cut(s) 465, 678
EcoRV GATATC 1 cut(s) 1189
EcoT14I CCWWGG 3 cut(s) 540, 561, 778
EcoT22I ATGCAT 2 cut(s) 76, 770
EcoT38I GRGCYC 2 cut(s) 467, 680
ErhI CCWWGG 3 cut(s) 540, 561, 778
FaeI CATG 1 cut(s) 74
FalI AAGNNNNNCTT 4 cut(s) 714, 746, 1472, 1504
FaqI GGGAC 2 cut(s) 1461, 1527
FatI CATG 1 cut(s) 70
FauI CCCGC 2 cut(s) 976, 1378
FblI GTMKAC 1 cut(s) 834
Fnu4HI GCNGC 6 cut(s) 345, 356, 450, 537, 981, 1379
FokI GGATG 4 cut(s) 578, 716, 1110, 1365
FriOI GRGCYC 2 cut(s) 467, 680
Fsp4HI GCNGC 6 cut(s) 345, 356, 450, 537, 981, 1379
FspBI CTAG 8 cut(s) 209, 299, 384, 531, 819, 1473, 1497, 1521
GluI GCNGC 6 cut(s) 345, 356, 450, 537, 981, 1379
GsuI CTGGAG 1 cut(s) 934
HaeIII GGCC 2 cut(s) 207, 1341
HapII CCGG 1 cut(s) 963
HgaI GACGC 1 cut(s) 1250
Hin1II CATG 1 cut(s) 74
HincII GTYRAC 1 cut(s) 1240
HindII GTYRAC 1 cut(s) 1240
HindIII AAGCTT 1 cut(s) 827
HinfI GANTC 9 cut(s) 15, 179, 482, 491, 1154, 1295, 1347, 1419, 1546
HpaII CCGG 1 cut(s) 963
HphI GGTGA 4 cut(s) 157, 1404, 1455, 1485
Hpy166II GTNNAC 3 cut(s) 835, 1102, 1240
Hpy188I TCNGA 6 cut(s) 64, 496, 579, 1003, 1153, 1352
Hpy188III TCNNGA 6 cut(s) 153, 510, 731, 963, 1013, 1299
Hpy8I GTNNAC 3 cut(s) 835, 1102, 1240
HpyAV CCTTC 7 cut(s) 136, 296, 427, 544, 565, 1158, 1470
HpyCH4III ACNGT 2 cut(s) 160, 1034
HpyF10VI GCNNNNNNNGC 5 cut(s) 295, 404, 977, 1241, 1440
HpyF3I CTNAG 8 cut(s) 63, 455, 525, 576, 867, 893, 1084, 1554
Hsp92II CATG 1 cut(s) 74
Kpn2I TCCGGA 1 cut(s) 962
Kzo9I GATC 5 cut(s) 197, 613, 751, 882, 1301
LguI GCTCTTC 1 cut(s) 1050
LmnI GCTCC 4 cut(s) 421, 462, 1090, 1334
Lsp1109I GCAGC 6 cut(s) 331, 342, 436, 523, 992, 1365
LweI GCATC 4 cut(s) 631, 777, 908, 1007
MaeI CTAG 8 cut(s) 209, 299, 384, 531, 819, 1473, 1497, 1521
MaeIII GTNAC 3 cut(s) 374, 856, 1410
MalI GATC 5 cut(s) 199, 615, 753, 884, 1303
MbiI CCGCTC 1 cut(s) 749
MboI GATC 5 cut(s) 197, 613, 751, 882, 1301
MboII GAAGA 7 cut(s) 682, 734, 746, 940, 1067, 1222, 1542
MfeI CAATTG 1 cut(s) 975
MflI RGATCY 3 cut(s) 197, 751, 882
MhlI GDGCHC 4 cut(s) 467, 680, 1062, 1178
MluCI AATT 6 cut(s) 362, 581, 975, 1289, 1382, 1540
MlyI GAGTC 2 cut(s) 1289, 1540
MmeI TCCRAC 2 cut(s) 681, 688
Mph1103I ATGCAT 2 cut(s) 76, 770
MroI TCCGGA 1 cut(s) 962
MseI TTAA 2 cut(s) 590, 666
MslI CAYNNNNRTG 2 cut(s) 627, 1454
MspA1I CMGCKG 1 cut(s) 983
MspI CCGG 1 cut(s) 963
MunI CAATTG 1 cut(s) 975
MvnI CGCG 1 cut(s) 330
MwoI GCNNNNNNNGC 5 cut(s) 295, 404, 977, 1241, 1440
NdeII GATC 5 cut(s) 197, 613, 751, 882, 1301
NlaIII CATG 1 cut(s) 74
NlaIV GGNNCC 2 cut(s) 874, 970
NmuCI GTSAC 2 cut(s) 856, 1410
NsiI ATGCAT 2 cut(s) 76, 770
NspI RCATGY 1 cut(s) 74
OliI CACNNNNGTG 1 cut(s) 1454
PciSI GCTCTTC 1 cut(s) 1050
PfeI GAWTC 7 cut(s) 15, 179, 482, 491, 1154, 1347, 1419
PflFI GACNNNGTC 1 cut(s) 1473
PkrI GCNGC 6 cut(s) 346, 357, 451, 538, 982, 1380
PleI GAGTC 2 cut(s) 1289, 1540
PpsI GAGTC 2 cut(s) 1289, 1540
PshAI GACNNNNGTC 1 cut(s) 1112
Psp124BI GAGCTC 2 cut(s) 467, 680
PspN4I GGNNCC 2 cut(s) 874, 970
PspPI GGNCC 2 cut(s) 782, 1340
PstI CTGCAG 2 cut(s) 360, 505
PstNI CAGNNNCTG 2 cut(s) 500, 1337
PsuI RGATCY 3 cut(s) 197, 751, 882
PsyI GACNNNGTC 1 cut(s) 1473
RsaI GTAC 2 cut(s) 776, 1216
RsaNI GTAC 2 cut(s) 775, 1215
RseI CAYNNNNRTG 2 cut(s) 627, 1454
SacI GAGCTC 2 cut(s) 467, 680
SapI GCTCTTC 1 cut(s) 1050
SaqAI TTAA 2 cut(s) 590, 666
SatI GCNGC 6 cut(s) 345, 356, 450, 537, 981, 1379
Sau3AI GATC 5 cut(s) 197, 613, 751, 882, 1301
Sau96I GGNCC 2 cut(s) 782, 1340
ScaI AGTACT 1 cut(s) 1216
SchI GAGTC 2 cut(s) 1289, 1540
SduI GDGCHC 4 cut(s) 467, 680, 1062, 1178
SfaNI GCATC 4 cut(s) 631, 777, 908, 1007
SfcI CTRYAG 5 cut(s) 356, 501, 1030, 1128, 1329
SinI GGWCC 1 cut(s) 782
SmiMI CAYNNNNRTG 2 cut(s) 627, 1454
SmlI CTYRAG 5 cut(s) 602, 731, 1013, 1181, 1394
SmoI CTYRAG 5 cut(s) 602, 731, 1013, 1181, 1394
Sse9I AATT 6 cut(s) 362, 581, 975, 1289, 1382, 1540
SsiI CCGC 5 cut(s) 460, 749, 920, 983, 1371
SspMI CTAG 8 cut(s) 209, 299, 384, 531, 819, 1473, 1497, 1521
SstI GAGCTC 2 cut(s) 467, 680
StyI CCWWGG 3 cut(s) 540, 561, 778
TaaI ACNGT 2 cut(s) 160, 1034
TaqI TCGA 5 cut(s) 273, 417, 625, 902, 1345
TasI AATT 6 cut(s) 362, 581, 975, 1289, 1382, 1540
TatI WGTACW 1 cut(s) 1214
TfiI GAWTC 7 cut(s) 15, 179, 482, 491, 1154, 1347, 1419
Tru1I TTAA 2 cut(s) 590, 666
Tru9I TTAA 2 cut(s) 590, 666
TscAI CASTG 4 cut(s) 901, 1039, 1441, 1461
TseFI GTSAC 2 cut(s) 856, 1410
TseI GCWGC 6 cut(s) 344, 355, 449, 536, 980, 1378
Tsp45I GTSAC 2 cut(s) 856, 1410
TspDTI ATGAA 4 cut(s) 354, 819, 1063, 1236
TspRI CASTG 4 cut(s) 901, 1039, 1441, 1461
Tth111I GACNNNGTC 1 cut(s) 1473
VpaK11BI GGWCC 1 cut(s) 782
XapI RAATTY 2 cut(s) 362, 1289
XceI RCATGY 1 cut(s) 74
XmiI GTMKAC 1 cut(s) 834
XspI CTAG 8 cut(s) 209, 299, 384, 531, 819, 1473, 1497, 1521
ZrmI AGTACT 1 cut(s) 1216
Zsp2I ATGCAT 2 cut(s) 76, 770
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.