MD09G1190800.v1.1

Short calmodulin-binding motif containing conserved Ile and Gln residues.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000760
Physical Location & Seq
Forward (+)
16671079 .. 16673550
2472 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1190800.v1.1.491

Sequence Viewer

Length: 1467 bp
ATGGTCACACTGACATCCATCAGTTTCCAGTCCAGGAAAAAGACGGCGACATCTGGAGCAGTTAGCGAGGAGAAAACTGATATGGGCAGCACCAATGAATCTAATGAGGTAGATAAAATGATTATTGAAGAATCCTTGAGCCCCAAGAAAAGAAAAGCAGGCCAGTTAAAGCTAGGGACAAATGTTTCTTTCAAGAAAATAGAAGGAAAAATGGATAGTTTTATTGAGGAGGTCAATCCAACAATTTCCTTACCCAAGCCAGAAGATTTCCTTTCTCCATGCCATCCTAGTGAGCTTCAGGCAGCTGCAACCAAAGTGCAGAAATTTTACAAGAGCTACCGGACTAGGCGGAACCTTGCAGATTGCGCTGTTGTAGTCGAGGAGCTATGGTGGAAGGCATTGGACTTTGCTGCTCTTAAACGCAGCTCTGTGTCATTCTTTAACTCTGATAAATCAGAAACTGCTGTCTCCAGATGGTCGCGGGCTAGGACTCGGGCTGCCAAGGTTGGAAAGGGTTTGTCAAAGGATGAGAAGGCTCAGGAATTAGCTCTAAGACATTGGCTTGAAGCGATTGATCCACGCCATCGTTACGGGCACAATCTGCACTTGTATTATGATGTCTGGTTTAACAGTGGGAGCTCTCAACCTTTCTTCTATTGGTTGGACGTTGGAGATGGAAAAGAAGAAAATCTTGAGAAGTGTGCTAGAACTGATTTGCAGCGTCAGTGCATCAAATACCTTGGACCGAAAGAGAGGGAAGCTTATGAAGTGATTGTACACGGCGGGCAGCTTGTGTACAGACAAAGTAGGAACCTTGTGAATACTGCTGCGGGTTCTAAATGGATCTTTGTCCTCGGCGCATCGAGGAACATGTACGTAGGGGAGAAGAAGAAAGGCTTTTTCCAACACTCCAGTTTTCTAGCAGGAGGTGCCACCATTGCAGCCGGGAGATTGGTTGCATGCAATGGGGTTCTCGAGGCTGTGTGGTGCTATAGCGGTCATTACCGCCCAACAGAAGAGAGTTTTTTGGAATTCATTAGCTTCCTAGAGGAGCATAAGGTGGACATGACAAATGTGAAGAAATGTCCTATCGATGATGATATTCCACCTTCGAAACCTCCGAAAAAAGTGATTAACACTGAGTCTACAGAAACTAAAGGCTCCACTTCCACTAATATAGCAAACAGTCAAAATGAACATAATGTGGATGGCGTCGGAACTATCGTTGAAGAAGCACCAGAATTCGACTCAGGCAGGCCTTTGTCTTGCAAATGGACTACCGGAGCCGGTCCTCGAATCGGATGTGTACGAGACTACCCAGCAGAGCTCCAAATTCAGGCACTTGAGCAGGTCAATCTATCACCAAGAGTTTCTGCCAGCAATGCTCCAATTCCTTCACCAAGACCAAGTCCAAAAATCCACCTGTCACCTAGGCTTACATGCATGGGACTGCTAAATCATCACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

489

Amino Acids

54.51

Weight (kDa)

8.81

Isoelectric Point (pI)

49.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1339
AccB1I GGYRCC 1 cut(s) 929
AccI GTMKAC 1 cut(s) 1145
AccII CGCG 1 cut(s) 481
AciI CCGC 6 cut(s) 349, 481, 783, 830, 996, 1006
AclWI GGATC 2 cut(s) 569, 851
AcsI RAATTY 4 cut(s) 323, 1031, 1241, 1332
AcuI CTGAAG 1 cut(s) 281
AcyI GRCGYC 1 cut(s) 1212
AfaI GTAC 4 cut(s) 777, 797, 875, 1308
AfiI CCNNNNNNNGG 2 cut(s) 1298, 1336
AflIII ACRYGT 1 cut(s) 870
AgsI TTSAA 4 cut(s) 128, 193, 566, 1229
AjnI CCWGG 1 cut(s) 32
Alw21I GWGCWC 2 cut(s) 641, 1329
Alw26I GTCTC 2 cut(s) 472, 1305
AlwI GGATC 2 cut(s) 569, 851
AlwNI CAGNNNCTG 1 cut(s) 461
Ama87I CYCGRG 2 cut(s) 492, 974
AoxI GGCC 2 cut(s) 160, 1256
ApoI RAATTY 4 cut(s) 323, 1031, 1241, 1332
AspA2I CCTAGG 1 cut(s) 1430
AspLEI GCGC 2 cut(s) 368, 860
AspS9I GGNCC 2 cut(s) 743, 1289
AsuC2I CCSGG 1 cut(s) 946
AsuHPI GGTGA 3 cut(s) 1353, 1389, 1419
AsuII TTCGAA 1 cut(s) 1112
AvaI CYCGRG 2 cut(s) 492, 974
AvaII GGWCC 2 cut(s) 743, 1289
AvrII CCTAGG 1 cut(s) 1430
BaeGI GKGCMC 1 cut(s) 597
BanI GGYRCC 1 cut(s) 929
BanII GRGCYC 3 cut(s) 143, 641, 1329
BarI GAAGNNNNNNTAC 2 cut(s) 759, 791
Bbv12I GWGCWC 2 cut(s) 641, 1329
BccI CCATC 6 cut(s) 26, 291, 468, 591, 668, 1202
BceAI ACGGC 2 cut(s) 60, 796
BciT130I CCWGG 1 cut(s) 34
BcnI CCSGG 1 cut(s) 946
BcoDI GTCTC 2 cut(s) 472, 1305
BfaI CTAG 9 cut(s) 173, 288, 345, 486, 705, 920, 1046, 1431, 1465
BfmI CTRYAG 2 cut(s) 991, 1146
BfuAI ACCTGC 1 cut(s) 1339
BlnI CCTAGG 1 cut(s) 1430
Bme1390I CCNGG 2 cut(s) 34, 946
Bme18I GGWCC 2 cut(s) 743, 1289
BmeT110I CYCGRG 2 cut(s) 492, 974
BmgT120I GGNCC 2 cut(s) 743, 1289
BmiI GGNNCC 5 cut(s) 353, 812, 931, 1162, 1285
BmrFI CCNGG 2 cut(s) 34, 946
BmsI GCATC 2 cut(s) 738, 869
BpmI CTGGAG 3 cut(s) 75, 454, 895
Bpu10I CCTNAGC 1 cut(s) 537
Bpu14I TTCGAA 1 cut(s) 1112
BpuEI CTTGAG 3 cut(s) 157, 713, 1364
BpuMI CCSGG 1 cut(s) 946
Bsa29I ATCGAT 1 cut(s) 1092
BsaAI YACGTR 1 cut(s) 877
BsaHI GRCGYC 1 cut(s) 1212
BsaJI CCNNGG 4 cut(s) 501, 739, 853, 1430
BsaWI WCCGGW 2 cut(s) 339, 1280
BsaXI ACNNNNNCTCC 2 cut(s) 374, 404
Bsc4I CCNNNNNNNGG 2 cut(s) 1298, 1336
Bse118I RCCGGY 1 cut(s) 1286
Bse1I ACTGG 3 cut(s) 28, 163, 912
Bse3DI GCAATG 3 cut(s) 936, 970, 1387
BseBI CCWGG 1 cut(s) 34
BseCI ATCGAT 1 cut(s) 1092
BseDI CCNNGG 4 cut(s) 501, 739, 853, 1430
BseGI GGATG 5 cut(s) 14, 283, 532, 1213, 1307
BseLI CCNNNNNNNGG 2 cut(s) 1298, 1336
BseMI GCAATG 3 cut(s) 936, 970, 1387
BseMII CTCAG 3 cut(s) 551, 1131, 1263
BseNI ACTGG 3 cut(s) 28, 163, 912
BseRI GAGGAG 4 cut(s) 83, 242, 395, 1064
BseSI GKGCMC 1 cut(s) 597
BseYI CCCAGC 1 cut(s) 1318
BsgI GTGCAG 2 cut(s) 338, 587
Bsh1236I CGCG 1 cut(s) 481
BshFI GGCC 2 cut(s) 162, 1258
BshNI GGYRCC 1 cut(s) 929
BshVI ATCGAT 1 cut(s) 1092
BsiHKAI GWGCWC 2 cut(s) 641, 1329
BsiHKCI CYCGRG 2 cut(s) 492, 974
BsiSI CCGG 4 cut(s) 340, 945, 1281, 1287
BslFI GGGAC 2 cut(s) 190, 1461
BslI CCNNNNNNNGG 2 cut(s) 1298, 1336
BsmAI GTCTC 2 cut(s) 472, 1305
BsmFI GGGAC 2 cut(s) 190, 1461
BsnI GGCC 2 cut(s) 162, 1258
BsoBI CYCGRG 2 cut(s) 492, 974
Bsp119I TTCGAA 1 cut(s) 1112
Bsp1286I GDGCHC 4 cut(s) 143, 597, 641, 1329
Bsp1407I TGTACA 2 cut(s) 775, 795
Bsp143I GATC 2 cut(s) 574, 843
BspACI CCGC 6 cut(s) 349, 481, 783, 830, 996, 1006
BspANI GGCC 2 cut(s) 162, 1258
BspCNI CTCAG 3 cut(s) 550, 1132, 1262
BspDI ATCGAT 1 cut(s) 1092
BspFNI CGCG 1 cut(s) 481
BspLI GGNNCC 5 cut(s) 353, 812, 931, 1162, 1285
BspMI ACCTGC 1 cut(s) 1339
BspPI GGATC 2 cut(s) 569, 851
BspT104I TTCGAA 1 cut(s) 1112
BspT107I GGYRCC 1 cut(s) 929
BsrDI GCAATG 3 cut(s) 936, 970, 1387
BsrFI RCCGGY 1 cut(s) 1286
BsrGI TGTACA 2 cut(s) 775, 795
BsrI ACTGG 3 cut(s) 28, 163, 912
BssAI RCCGGY 1 cut(s) 1286
BssECI CCNNGG 4 cut(s) 501, 739, 853, 1430
BssMI GATC 2 cut(s) 574, 843
BssNI GRCGYC 1 cut(s) 1212
BssT1I CCWWGG 3 cut(s) 501, 739, 1430
Bst2UI CCWGG 1 cut(s) 34
Bst4CI ACNGT 2 cut(s) 632, 1187
Bst6I CTCTTC 1 cut(s) 1011
BstACI GRCGYC 1 cut(s) 1212
BstAPI GCANNNNNTGC 2 cut(s) 601, 929
BstAUI TGTACA 2 cut(s) 775, 795
BstBAI YACGTR 1 cut(s) 877
BstBI TTCGAA 1 cut(s) 1112
BstC8I GCNNGC 6 cut(s) 160, 483, 785, 961, 1256, 1378
BstDEI CTNAG 4 cut(s) 537, 551, 1140, 1249
BstF5I GGATG 5 cut(s) 14, 283, 532, 1213, 1307
BstFNI CGCG 1 cut(s) 481
BstHHI GCGC 2 cut(s) 368, 860
BstKTI GATC 2 cut(s) 577, 846
BstMAI GTCTC 2 cut(s) 472, 1305
BstMBI GATC 2 cut(s) 574, 843
BstMWI GCNNNNNNNGC 5 cut(s) 365, 601, 929, 938, 1382
BstNI CCWGG 1 cut(s) 34
BstNSI RCATGY 3 cut(s) 874, 963, 1443
BstSCI CCNGG 2 cut(s) 32, 944
BstSFI CTRYAG 2 cut(s) 991, 1146
BstSLI GKGCMC 1 cut(s) 597
BstSNI TACGTA 1 cut(s) 877
BstUI CGCG 1 cut(s) 481
BstX2I RGATCY 1 cut(s) 843
BstYI RGATCY 1 cut(s) 843
Bsu15I ATCGAT 1 cut(s) 1092
BsuRI GGCC 2 cut(s) 162, 1258
BsuTUI ATCGAT 1 cut(s) 1092
BtsCI GGATG 5 cut(s) 14, 283, 532, 1213, 1307
BtsIMutI CAGTG 4 cut(s) 8, 637, 731, 1137
BveI ACCTGC 1 cut(s) 1339
Cac8I GCNNGC 6 cut(s) 160, 483, 785, 961, 1256, 1378
CaiI CAGNNNCTG 1 cut(s) 461
CfoI GCGC 2 cut(s) 368, 860
Cfr10I RCCGGY 1 cut(s) 1286
Cfr13I GGNCC 2 cut(s) 743, 1289
ClaI ATCGAT 1 cut(s) 1092
CseI GACGC 2 cut(s) 710, 1201
Csp6I GTAC 4 cut(s) 776, 796, 874, 1307
CviAII CATG 6 cut(s) 279, 871, 960, 1066, 1440, 1444
CviQI GTAC 4 cut(s) 776, 796, 874, 1307
DdeI CTNAG 4 cut(s) 537, 551, 1140, 1249
DpnI GATC 2 cut(s) 576, 845
DpnII GATC 2 cut(s) 574, 843
Eam1104I CTCTTC 1 cut(s) 1011
EarI CTCTTC 1 cut(s) 1011
EciI GGCGGA 1 cut(s) 364
Ecl136II GAGCTC 2 cut(s) 639, 1327
Eco105I TACGTA 1 cut(s) 877
Eco130I CCWWGG 3 cut(s) 501, 739, 1430
Eco147I AGGCCT 1 cut(s) 1258
Eco24I GRGCYC 3 cut(s) 143, 641, 1329
Eco47I GGWCC 2 cut(s) 743, 1289
Eco53kI GAGCTC 2 cut(s) 639, 1327
Eco57I CTGAAG 1 cut(s) 281
Eco88I CYCGRG 2 cut(s) 492, 974
EcoICRI GAGCTC 2 cut(s) 639, 1327
EcoRI GAATTC 2 cut(s) 1031, 1241
EcoRII CCWGG 1 cut(s) 32
EcoT14I CCWWGG 3 cut(s) 501, 739, 1430
EcoT22I ATGCAT 1 cut(s) 1445
EcoT38I GRGCYC 3 cut(s) 143, 641, 1329
ErhI CCWWGG 3 cut(s) 501, 739, 1430
FaeI CATG 6 cut(s) 282, 874, 963, 1069, 1443, 1447
FalI AAGNNNNNCTT 6 cut(s) 255, 287, 675, 707, 881, 913
FaqI GGGAC 2 cut(s) 190, 1461
FatI CATG 6 cut(s) 278, 870, 959, 1065, 1439, 1443
FauI CCCGC 3 cut(s) 474, 776, 823
FblI GTMKAC 1 cut(s) 1145
FokI GGATG 4 cut(s) 270, 539, 1220, 1314
FriOI GRGCYC 3 cut(s) 143, 641, 1329
FspBI CTAG 9 cut(s) 173, 288, 345, 486, 705, 920, 1046, 1431, 1465
GlaI GCGC 2 cut(s) 367, 859
GsaI CCCAGC 1 cut(s) 1322
GsuI CTGGAG 3 cut(s) 75, 454, 895
HaeIII GGCC 2 cut(s) 162, 1258
HapII CCGG 4 cut(s) 340, 945, 1281, 1287
HgaI GACGC 2 cut(s) 710, 1201
HhaI GCGC 2 cut(s) 368, 860
Hin1I GRCGYC 1 cut(s) 1212
Hin1II CATG 6 cut(s) 282, 874, 963, 1069, 1443, 1447
Hin6I GCGC 2 cut(s) 366, 858
HinP1I GCGC 2 cut(s) 366, 858
HindIII AAGCTT 1 cut(s) 759
HinfI GANTC 6 cut(s) 98, 131, 490, 1142, 1247, 1296
HpaII CCGG 4 cut(s) 340, 945, 1281, 1287
HphI GGTGA 3 cut(s) 1353, 1389, 1419
Hpy166II GTNNAC 5 cut(s) 778, 796, 1063, 1146, 1307
Hpy188I TCNGA 5 cut(s) 448, 457, 1122, 1217, 1301
Hpy188III TCNNGA 6 cut(s) 54, 193, 471, 539, 692, 974
Hpy8I GTNNAC 5 cut(s) 778, 796, 1063, 1146, 1307
Hpy99I CGWCG 1 cut(s) 1217
HpyAV CCTTC 5 cut(s) 197, 388, 526, 1119, 1404
HpyCH4III ACNGT 2 cut(s) 632, 1187
HpyCH4IV ACGT 2 cut(s) 666, 876
HpyF10VI GCNNNNNNNGC 5 cut(s) 365, 601, 929, 938, 1382
HpyF3I CTNAG 4 cut(s) 537, 551, 1140, 1249
HpySE526I ACGT 2 cut(s) 666, 876
Hsp92I GRCGYC 1 cut(s) 1212
Hsp92II CATG 6 cut(s) 282, 874, 963, 1069, 1443, 1447
HspAI GCGC 2 cut(s) 366, 858
Kzo9I GATC 2 cut(s) 574, 843
LmnI GCTCC 8 cut(s) 56, 382, 636, 1051, 1166, 1283, 1332, 1390
LweI GCATC 2 cut(s) 738, 869
MaeI CTAG 9 cut(s) 173, 288, 345, 486, 705, 920, 1046, 1431, 1465
MaeII ACGT 2 cut(s) 666, 876
MaeIII GTNAC 3 cut(s) 4, 587, 1425
MalI GATC 2 cut(s) 576, 845
MboI GATC 2 cut(s) 574, 843
MboII GAAGA 9 cut(s) 140, 275, 643, 695, 898, 901, 1028, 1090, 1241
MflI RGATCY 1 cut(s) 843
MhlI GDGCHC 4 cut(s) 143, 597, 641, 1329
MluCI AATT 7 cut(s) 243, 323, 542, 1031, 1241, 1332, 1389
MlyI GAGTC 3 cut(s) 484, 1151, 1241
MmeI TCCRAC 6 cut(s) 263, 487, 642, 649, 928, 1195
Mph1103I ATGCAT 1 cut(s) 1445
MseI TTAA 5 cut(s) 167, 417, 441, 627, 1134
MslI CAYNNNNRTG 1 cut(s) 288
MspA1I CMGCKG 1 cut(s) 305
MspI CCGG 4 cut(s) 340, 945, 1281, 1287
MspR9I CCNGG 2 cut(s) 34, 946
MvaI CCWGG 1 cut(s) 34
MvnI CGCG 1 cut(s) 481
MwoI GCNNNNNNNGC 5 cut(s) 365, 601, 929, 938, 1382
NciI CCSGG 1 cut(s) 946
NdeII GATC 2 cut(s) 574, 843
NlaIII CATG 6 cut(s) 282, 874, 963, 1069, 1443, 1447
NlaIV GGNNCC 5 cut(s) 353, 812, 931, 1162, 1285
NmeAIII GCCGAG 1 cut(s) 834
NmuCI GTSAC 2 cut(s) 4, 1425
NsiI ATGCAT 1 cut(s) 1445
NspI RCATGY 3 cut(s) 874, 963, 1443
NspV TTCGAA 1 cut(s) 1112
PaeI GCATGC 1 cut(s) 963
PaeR7I CTCGAG 1 cut(s) 974
PceI AGGCCT 1 cut(s) 1258
PciI ACATGT 1 cut(s) 870
PcsI WCGNNNNNNNCGW 2 cut(s) 1118, 1221
PfeI GAWTC 3 cut(s) 98, 131, 1296
PflFI GACNNNGTC 1 cut(s) 1407
PfoI TCCNGGA 1 cut(s) 32
PleI GAGTC 3 cut(s) 484, 1150, 1241
PpsI GAGTC 3 cut(s) 484, 1150, 1241
Ppu21I YACGTR 1 cut(s) 877
PscI ACATGT 1 cut(s) 870
Psp124BI GAGCTC 2 cut(s) 641, 1329
Psp6I CCWGG 1 cut(s) 32
PspFI CCCAGC 1 cut(s) 1318
PspGI CCWGG 1 cut(s) 32
PspN4I GGNNCC 5 cut(s) 353, 812, 931, 1162, 1285
PspPI GGNCC 2 cut(s) 743, 1289
PstNI CAGNNNCTG 1 cut(s) 461
PsuI RGATCY 1 cut(s) 843
PsyI GACNNNGTC 1 cut(s) 1407
PvuII CAGCTG 1 cut(s) 305
RsaI GTAC 4 cut(s) 777, 797, 875, 1308
RsaNI GTAC 4 cut(s) 776, 796, 874, 1307
RseI CAYNNNNRTG 1 cut(s) 288
SacI GAGCTC 2 cut(s) 641, 1329
SaqAI TTAA 5 cut(s) 167, 417, 441, 627, 1134
Sau3AI GATC 2 cut(s) 574, 843
Sau96I GGNCC 2 cut(s) 743, 1289
SchI GAGTC 3 cut(s) 484, 1151, 1241
ScrFI CCNGG 2 cut(s) 34, 946
SduI GDGCHC 4 cut(s) 143, 597, 641, 1329
SfaNI GCATC 2 cut(s) 738, 869
SfcI CTRYAG 2 cut(s) 991, 1146
Sfr274I CTCGAG 1 cut(s) 974
SfuI TTCGAA 1 cut(s) 1112
SinI GGWCC 2 cut(s) 743, 1289
SlaI CTCGAG 1 cut(s) 974
SmiMI CAYNNNNRTG 1 cut(s) 288
SmlI CTYRAG 4 cut(s) 136, 692, 974, 1343
SmoI CTYRAG 4 cut(s) 136, 692, 974, 1343
SnaBI TACGTA 1 cut(s) 877
SphI GCATGC 1 cut(s) 963
Sse9I AATT 7 cut(s) 243, 323, 542, 1031, 1241, 1332, 1389
SseBI AGGCCT 1 cut(s) 1258
SsiI CCGC 6 cut(s) 349, 481, 783, 830, 996, 1006
SspMI CTAG 9 cut(s) 173, 288, 345, 486, 705, 920, 1046, 1431, 1465
SstI GAGCTC 2 cut(s) 641, 1329
StuI AGGCCT 1 cut(s) 1258
StyD4I CCNGG 2 cut(s) 32, 944
StyI CCWWGG 3 cut(s) 501, 739, 1430
TaaI ACNGT 2 cut(s) 632, 1187
TaiI ACGT 2 cut(s) 669, 879
TaqI TCGA 7 cut(s) 378, 863, 975, 1092, 1112, 1245, 1294
TaqII GACCGA 1 cut(s) 760
TasI AATT 7 cut(s) 243, 323, 542, 1031, 1241, 1332, 1389
TatI WGTACW 2 cut(s) 775, 795
TfiI GAWTC 3 cut(s) 98, 131, 1296
Tru1I TTAA 5 cut(s) 167, 417, 441, 627, 1134
Tru9I TTAA 5 cut(s) 167, 417, 441, 627, 1134
TscAI CASTG 4 cut(s) 15, 637, 731, 1144
TseFI GTSAC 2 cut(s) 4, 1425
Tsp45I GTSAC 2 cut(s) 4, 1425
TspDTI ATGAA 4 cut(s) 111, 780, 1024, 1209
TspRI CASTG 4 cut(s) 15, 637, 731, 1144
Tth111I GACNNNGTC 1 cut(s) 1407
VpaK11BI GGWCC 2 cut(s) 743, 1289
XapI RAATTY 4 cut(s) 323, 1031, 1241, 1332
XceI RCATGY 3 cut(s) 874, 963, 1443
XhoI CTCGAG 1 cut(s) 974
XmaJI CCTAGG 1 cut(s) 1430
XmiI GTMKAC 1 cut(s) 1145
XspI CTAG 9 cut(s) 173, 288, 345, 486, 705, 920, 1046, 1431, 1465
Zsp2I ATGCAT 1 cut(s) 1445
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.