Rroxscaffold_2G00101510

Short calmodulin-binding motif containing conserved Ile and Gln residues.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000760
Physical Location & Seq
Forward (+)
23440834 .. 23443512
2679 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00101510.1

Sequence Viewer

Length: 1545 bp
ATGGAGATAATGAGATTCACTACCTTGAATGTGGATTTTCTTATGATACAAACACATATTTCTGAGGCACATGCATTTTGTTTTGACAGAACTTGTAGAATGGTGACAATGACAGCTATAAGTTTAGTGTCCAAGAGAACGAAGGTGATGTCAAGAACAGTTAGTTTTGAGAACACCGATTCAAGCAATACCAACAGATCCGATGGCCTAGATACATTGGTTTTTGAAAAATCTGTATGCTACAAGAAAAGGAAAGCAGGGCAACTAACGCTCGATACTTCAACTTTGCTACCCAAGCTAGAAGGTTGTGTTTCTCCACAGACCCCACGCGAACTTGATGAAGCTGCAATCAAGCTGCAGAAATTTTACAAGAGTTACCGAACTAGACGAAACCTTGCAGATTGTGCTGTTGTTGTCGAGGAGCTATGGTGGAAGGCATTAGACTTTGCTGCACTTAGGCGGAGCTCTGTCTCATTCTTTGAATCTGTTGAATCAGAAACTGCAGTTTCCAGATGGGCAAGGGCTAAGACTAGGGCTGCCAAGGTTGGGAAGGGTTTGTCCAAGGATGAGAAGGCTCAGAAATTAGCTTTAAGACATTGGCTTGAGGCTATTGATCCACGGCATCGATATGGACATAATTTGCACTTGTATTATGATGTCTGGTTTAATAGTGCGAGCTCTCAACCTTTCTTCTACTGGTTGGATGTTGGAGATGGTAAAGAAGAAAATCTTGAGAAGTGTCCAAGAGCGGATCTTCAACGCCAATGCATCAAGTACCTTGGACCGAATGAGAGGGAAGCATATGAAGTGATAGTGGCAAGTGGGAAGCTTGTATACAGACAAAGTGGCAACCTTGTGACTACTACTGAGGGTTCCAAATGGATCTTTATTCTCAGTGCATCGAGGATTATGTATGTCGCGGAGAAGAAGAAAGGCTATTTCCAGCACTCTAGTTTTCTATCCGGAGGTGCCACAATTGCAGCGGGGAGATTAGTTGCTTCTGATGGGGTTCTTGAGGCTGTATGGTGCTACAGTGGTCATTATCGCCCTACAGAAGAGCACTTTTTGGAGTTCATTAGCTTCTTAGAGGAGCAACAGGTGGACTTGACAAATGTCAAAAAACATCCTATAGATGATGATATTCCACCTTCAGAATCTGCCAGTAAAGATATGAGCACCCTTGAGGATATCAAATCGGCAAACATTGAGACGAGTACTAATGAAAATGAGCGTGCCATTGACGCTTCCATAGTAAGTGATGACAATCATCAAGACACTGTGGAAGCACCAAAATTTGACTCGGGAAGGGGATTGCCTTGCAAATGGACAACAGGAGCTGGTCCTCGAATCGGATGTGTGAGAGACTACCCCGCACAGCTACAATTTCAGGCACTAGAGCAAGTCAATCTGTCACCTAGAATAAAACCAGAACGCACTGCAAGCAATAATACCCCAGTGCCTTCACCAAGACCTAGTCCCAAGTTCCACTTATCACCTAGACTTTCATATATGGGACTTCCTAGCCCCAGAAGACTGCATAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

514

Amino Acids

58.21

Weight (kDa)

8.52

Isoelectric Point (pI)

44.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 968
AccBSI CCGCTC 1 cut(s) 749
AccI GTMKAC 1 cut(s) 834
AccII CGCG 2 cut(s) 330, 920
AccIII TCCGGA 1 cut(s) 962
AciI CCGC 5 cut(s) 460, 749, 920, 983, 1371
AclWI GGATC 4 cut(s) 192, 608, 759, 890
AcsI RAATTY 2 cut(s) 362, 1292
AcuI CTGAAG 1 cut(s) 1134
AfaI GTAC 2 cut(s) 776, 1216
AfiI CCNNNNNNNGG 2 cut(s) 546, 1349
AgsI TTSAA 7 cut(s) 28, 183, 227, 282, 482, 491, 758
Alw21I GWGCWC 4 cut(s) 467, 680, 1062, 1178
Alw26I GTCTC 3 cut(s) 475, 1202, 1356
AlwI GGATC 4 cut(s) 192, 608, 759, 890
AlwNI CAGNNNCTG 3 cut(s) 500, 1157, 1337
Ama87I CYCGRG 1 cut(s) 1300
Aor13HI TCCGGA 1 cut(s) 962
AoxI GGCC 1 cut(s) 205
ApeKI GCWGC 5 cut(s) 344, 355, 449, 536, 980
ApoI RAATTY 2 cut(s) 362, 1292
ArsI GACNNNNNNTTYG 2 cut(s) 134, 166
AspS9I GGNCC 2 cut(s) 782, 1340
AsuHPI GGTGA 5 cut(s) 115, 157, 1404, 1455, 1485
AvaI CYCGRG 1 cut(s) 1300
AvaII GGWCC 2 cut(s) 782, 1340
BaeI ACNNNNGTAYC 2 cut(s) 204, 237
BanI GGYRCC 1 cut(s) 968
BanII GRGCYC 2 cut(s) 467, 680
BarI GAAGNNNNNNTAC 2 cut(s) 818, 850
BbsI GAAGAC 1 cut(s) 1537
Bbv12I GWGCWC 4 cut(s) 467, 680, 1062, 1178
BbvI GCAGC 5 cut(s) 331, 342, 436, 523, 992
BccI CCATC 4 cut(s) 197, 507, 707, 998
BceAI ACGGC 1 cut(s) 635
BcoDI GTCTC 3 cut(s) 475, 1202, 1356
BfmI CTRYAG 5 cut(s) 356, 501, 1030, 1050, 1128
BisI GCNGC 5 cut(s) 345, 356, 450, 537, 981
BlsI GCNGC 5 cut(s) 346, 357, 451, 538, 982
BmcAI AGTACT 1 cut(s) 1216
Bme18I GGWCC 2 cut(s) 782, 1340
BmeT110I CYCGRG 1 cut(s) 1300
BmgT120I GGNCC 2 cut(s) 782, 1340
BmiI GGNNCC 2 cut(s) 874, 970
BmrI ACTGGG 1 cut(s) 1448
BmsI GCATC 3 cut(s) 631, 777, 908
BmuI ACTGGG 1 cut(s) 1448
BoxI GACNNNNGTC 1 cut(s) 1112
BpiI GAAGAC 1 cut(s) 1537
BpuEI CTTGAG 4 cut(s) 623, 752, 1034, 1202
Bsa29I ATCGAT 1 cut(s) 625
BsaBI GATNNNNATC 1 cut(s) 1263
BsaJI CCNNGG 4 cut(s) 540, 561, 617, 778
BsaWI WCCGGW 1 cut(s) 962
BsaXI ACNNNNNCTCC 2 cut(s) 413, 443
Bsc4I CCNNNNNNNGG 2 cut(s) 546, 1349
Bse1I ACTGG 3 cut(s) 701, 1161, 1454
Bse8I GATNNNNATC 1 cut(s) 1263
BseAI TCCGGA 1 cut(s) 962
BseCI ATCGAT 1 cut(s) 625
BseDI CCNNGG 4 cut(s) 540, 561, 617, 778
BseGI GGATG 4 cut(s) 571, 709, 1123, 1358
BseJI GATNNNNATC 1 cut(s) 1263
BseLI CCNNNNNNNGG 2 cut(s) 546, 1349
BseMII CTCAG 4 cut(s) 54, 590, 858, 907
BseNI ACTGG 3 cut(s) 701, 1161, 1454
BseRI GAGGAG 2 cut(s) 434, 1103
BseXI GCAGC 5 cut(s) 331, 342, 436, 523, 992
BsgI GTGCAG 1 cut(s) 435
Bsh1236I CGCG 2 cut(s) 330, 920
BshFI GGCC 1 cut(s) 207
BshNI GGYRCC 1 cut(s) 968
BshVI ATCGAT 1 cut(s) 625
BsiHKAI GWGCWC 4 cut(s) 467, 680, 1062, 1178
BsiHKCI CYCGRG 1 cut(s) 1300
BsiSI CCGG 1 cut(s) 963
BslFI GGGAC 2 cut(s) 1461, 1527
BslI CCNNNNNNNGG 2 cut(s) 546, 1349
BsmAI GTCTC 3 cut(s) 475, 1202, 1356
BsmBI CGTCTC 1 cut(s) 1202
BsmFI GGGAC 2 cut(s) 1461, 1527
BsnI GGCC 1 cut(s) 207
BsoBI CYCGRG 1 cut(s) 1300
Bsp1286I GDGCHC 4 cut(s) 467, 680, 1062, 1178
Bsp13I TCCGGA 1 cut(s) 962
Bsp143I GATC 4 cut(s) 197, 613, 751, 882
BspACI CCGC 5 cut(s) 460, 749, 920, 983, 1371
BspANI GGCC 1 cut(s) 207
BspCNI CTCAG 4 cut(s) 55, 589, 859, 906
BspDI ATCGAT 1 cut(s) 625
BspEI TCCGGA 1 cut(s) 962
BspFNI CGCG 2 cut(s) 330, 920
BspLI GGNNCC 2 cut(s) 874, 970
BspMAI CTGCAG 2 cut(s) 360, 505
BspPI GGATC 4 cut(s) 192, 608, 759, 890
BspQI GCTCTTC 1 cut(s) 1050
BspT107I GGYRCC 1 cut(s) 968
BsrBI CCGCTC 1 cut(s) 749
BsrI ACTGG 3 cut(s) 701, 1161, 1454
BssECI CCNNGG 4 cut(s) 540, 561, 617, 778
BssMI GATC 4 cut(s) 197, 613, 751, 882
BssNAI GTATAC 1 cut(s) 835
BssT1I CCWWGG 3 cut(s) 540, 561, 778
Bst1107I GTATAC 1 cut(s) 835
Bst4CI ACNGT 3 cut(s) 160, 1034, 1279
Bst6I CTCTTC 1 cut(s) 1050
BstAPI GCANNNNNTGC 1 cut(s) 404
BstC8I GCNNGC 3 cut(s) 676, 1233, 1441
BstDEI CTNAG 7 cut(s) 63, 455, 525, 576, 867, 893, 1084
BstDSI CCRYGG 1 cut(s) 617
BstF5I GGATG 4 cut(s) 571, 709, 1123, 1358
BstFNI CGCG 2 cut(s) 330, 920
BstKTI GATC 4 cut(s) 200, 616, 754, 885
BstMAI GTCTC 3 cut(s) 475, 1202, 1356
BstMBI GATC 4 cut(s) 197, 613, 751, 882
BstMWI GCNNNNNNNGC 6 cut(s) 268, 295, 404, 977, 1241, 1440
BstNSI RCATGY 1 cut(s) 74
BstPAI GACNNNNGTC 1 cut(s) 1112
BstSFI CTRYAG 5 cut(s) 356, 501, 1030, 1050, 1128
BstUI CGCG 2 cut(s) 330, 920
BstV1I GCAGC 5 cut(s) 331, 342, 436, 523, 992
BstV2I GAAGAC 1 cut(s) 1537
BstX2I RGATCY 3 cut(s) 197, 751, 882
BstYI RGATCY 3 cut(s) 197, 751, 882
BstZ17I GTATAC 1 cut(s) 835
Bsu15I ATCGAT 1 cut(s) 625
BsuRI GGCC 1 cut(s) 207
BsuTUI ATCGAT 1 cut(s) 625
BtgI CCRYGG 1 cut(s) 617
BtsCI GGATG 4 cut(s) 571, 709, 1123, 1358
BtsI GCAGTG 1 cut(s) 1434
BtsIMutI CAGTG 5 cut(s) 901, 1039, 1275, 1434, 1461
Cac8I GCNNGC 3 cut(s) 676, 1233, 1441
CaiI CAGNNNCTG 3 cut(s) 500, 1157, 1337
Cfr13I GGNCC 2 cut(s) 782, 1340
ClaI ATCGAT 1 cut(s) 625
CseI GACGC 1 cut(s) 1250
Csp6I GTAC 2 cut(s) 775, 1215
CviAII CATG 1 cut(s) 71
CviQI GTAC 2 cut(s) 775, 1215
DdeI CTNAG 7 cut(s) 63, 455, 525, 576, 867, 893, 1084
DpnI GATC 4 cut(s) 199, 615, 753, 884
DpnII GATC 4 cut(s) 197, 613, 751, 882
Eam1104I CTCTTC 1 cut(s) 1050
EarI CTCTTC 1 cut(s) 1050
EciI GGCGGA 1 cut(s) 475
Ecl136II GAGCTC 2 cut(s) 465, 678
Eco130I CCWWGG 3 cut(s) 540, 561, 778
Eco24I GRGCYC 2 cut(s) 467, 680
Eco32I GATATC 1 cut(s) 1189
Eco47I GGWCC 2 cut(s) 782, 1340
Eco53kI GAGCTC 2 cut(s) 465, 678
Eco57I CTGAAG 1 cut(s) 1134
Eco88I CYCGRG 1 cut(s) 1300
EcoICRI GAGCTC 2 cut(s) 465, 678
EcoRV GATATC 1 cut(s) 1189
EcoT14I CCWWGG 3 cut(s) 540, 561, 778
EcoT22I ATGCAT 2 cut(s) 76, 770
EcoT38I GRGCYC 2 cut(s) 467, 680
ErhI CCWWGG 3 cut(s) 540, 561, 778
Esp3I CGTCTC 1 cut(s) 1202
FaeI CATG 1 cut(s) 74
FalI AAGNNNNNCTT 4 cut(s) 714, 746, 1472, 1504
FaqI GGGAC 2 cut(s) 1461, 1527
FatI CATG 1 cut(s) 70
FauI CCCGC 2 cut(s) 976, 1378
FauNDI CATATG 1 cut(s) 802
FblI GTMKAC 1 cut(s) 834
Fnu4HI GCNGC 5 cut(s) 345, 356, 450, 537, 981
FokI GGATG 4 cut(s) 578, 716, 1110, 1365
FriOI GRGCYC 2 cut(s) 467, 680
Fsp4HI GCNGC 5 cut(s) 345, 356, 450, 537, 981
GluI GCNGC 5 cut(s) 345, 356, 450, 537, 981
HaeIII GGCC 1 cut(s) 207
HapII CCGG 1 cut(s) 963
HgaI GACGC 1 cut(s) 1250
Hin1II CATG 1 cut(s) 74
HindIII AAGCTT 1 cut(s) 827
HinfI GANTC 7 cut(s) 15, 179, 482, 491, 1154, 1298, 1347
HpaII CCGG 1 cut(s) 963
HphI GGTGA 5 cut(s) 115, 157, 1404, 1455, 1485
Hpy166II GTNNAC 2 cut(s) 835, 1102
Hpy188I TCNGA 7 cut(s) 64, 202, 496, 579, 1003, 1153, 1352
Hpy188III TCNNGA 7 cut(s) 153, 510, 731, 963, 1013, 1271, 1302
Hpy8I GTNNAC 2 cut(s) 835, 1102
HpyAV CCTTC 8 cut(s) 136, 296, 427, 544, 565, 1158, 1299, 1470
HpyCH4III ACNGT 3 cut(s) 160, 1034, 1279
HpyF10VI GCNNNNNNNGC 6 cut(s) 268, 295, 404, 977, 1241, 1440
HpyF3I CTNAG 7 cut(s) 63, 455, 525, 576, 867, 893, 1084
Hsp92II CATG 1 cut(s) 74
Kpn2I TCCGGA 1 cut(s) 962
Kzo9I GATC 4 cut(s) 197, 613, 751, 882
LguI GCTCTTC 1 cut(s) 1050
LmnI GCTCC 4 cut(s) 421, 462, 1090, 1334
Lsp1109I GCAGC 5 cut(s) 331, 342, 436, 523, 992
LweI GCATC 3 cut(s) 631, 777, 908
MaeIII GTNAC 4 cut(s) 103, 374, 856, 1410
MalI GATC 4 cut(s) 199, 615, 753, 884
MbiI CCGCTC 1 cut(s) 749
MboI GATC 4 cut(s) 197, 613, 751, 882
MboII GAAGA 7 cut(s) 682, 734, 746, 937, 940, 1067, 1542
MfeI CAATTG 1 cut(s) 975
MflI RGATCY 3 cut(s) 197, 751, 882
MhlI GDGCHC 4 cut(s) 467, 680, 1062, 1178
MluCI AATT 7 cut(s) 362, 581, 637, 975, 1292, 1382, 1540
MlyI GAGTC 1 cut(s) 1292
MmeI TCCRAC 2 cut(s) 681, 688
Mph1103I ATGCAT 2 cut(s) 76, 770
MroI TCCGGA 1 cut(s) 962
MseI TTAA 2 cut(s) 590, 666
MslI CAYNNNNRTG 1 cut(s) 627
MspA1I CMGCKG 1 cut(s) 983
MspI CCGG 1 cut(s) 963
MunI CAATTG 1 cut(s) 975
MvnI CGCG 2 cut(s) 330, 920
MwoI GCNNNNNNNGC 6 cut(s) 268, 295, 404, 977, 1241, 1440
NdeI CATATG 1 cut(s) 802
NdeII GATC 4 cut(s) 197, 613, 751, 882
NlaIII CATG 1 cut(s) 74
NlaIV GGNNCC 2 cut(s) 874, 970
NmuCI GTSAC 3 cut(s) 103, 856, 1410
NsiI ATGCAT 2 cut(s) 76, 770
NspI RCATGY 1 cut(s) 74
PciSI GCTCTTC 1 cut(s) 1050
PfeI GAWTC 6 cut(s) 15, 179, 482, 491, 1154, 1347
PflFI GACNNNGTC 1 cut(s) 1473
PkrI GCNGC 5 cut(s) 346, 357, 451, 538, 982
PleI GAGTC 1 cut(s) 1292
PpsI GAGTC 1 cut(s) 1292
PshAI GACNNNNGTC 1 cut(s) 1112
Psp124BI GAGCTC 2 cut(s) 467, 680
PspN4I GGNNCC 2 cut(s) 874, 970
PspPI GGNCC 2 cut(s) 782, 1340
PstI CTGCAG 2 cut(s) 360, 505
PstNI CAGNNNCTG 3 cut(s) 500, 1157, 1337
PsuI RGATCY 3 cut(s) 197, 751, 882
PsyI GACNNNGTC 1 cut(s) 1473
RsaI GTAC 2 cut(s) 776, 1216
RsaNI GTAC 2 cut(s) 775, 1215
RseI CAYNNNNRTG 1 cut(s) 627
SacI GAGCTC 2 cut(s) 467, 680
SapI GCTCTTC 1 cut(s) 1050
SaqAI TTAA 2 cut(s) 590, 666
SatI GCNGC 5 cut(s) 345, 356, 450, 537, 981
Sau3AI GATC 4 cut(s) 197, 613, 751, 882
Sau96I GGNCC 2 cut(s) 782, 1340
ScaI AGTACT 1 cut(s) 1216
SchI GAGTC 1 cut(s) 1292
SduI GDGCHC 4 cut(s) 467, 680, 1062, 1178
SfaNI GCATC 3 cut(s) 631, 777, 908
SfcI CTRYAG 5 cut(s) 356, 501, 1030, 1050, 1128
SinI GGWCC 2 cut(s) 782, 1340
SmiMI CAYNNNNRTG 1 cut(s) 627
SmlI CTYRAG 4 cut(s) 602, 731, 1013, 1181
SmoI CTYRAG 4 cut(s) 602, 731, 1013, 1181
Sse9I AATT 7 cut(s) 362, 581, 637, 975, 1292, 1382, 1540
SsiI CCGC 5 cut(s) 460, 749, 920, 983, 1371
SstI GAGCTC 2 cut(s) 467, 680
StyI CCWWGG 3 cut(s) 540, 561, 778
TaaI ACNGT 3 cut(s) 160, 1034, 1279
TaqI TCGA 5 cut(s) 273, 417, 625, 902, 1345
TaqII GACCGA 1 cut(s) 799
TasI AATT 7 cut(s) 362, 581, 637, 975, 1292, 1382, 1540
TatI WGTACW 1 cut(s) 1214
TfiI GAWTC 6 cut(s) 15, 179, 482, 491, 1154, 1347
Tru1I TTAA 2 cut(s) 590, 666
Tru9I TTAA 2 cut(s) 590, 666
TscAI CASTG 5 cut(s) 901, 1039, 1282, 1441, 1461
TseFI GTSAC 3 cut(s) 103, 856, 1410
TseI GCWGC 5 cut(s) 344, 355, 449, 536, 980
Tsp45I GTSAC 3 cut(s) 103, 856, 1410
TspDTI ATGAA 5 cut(s) 354, 819, 1063, 1236, 1494
TspRI CASTG 5 cut(s) 901, 1039, 1282, 1441, 1461
Tth111I GACNNNGTC 1 cut(s) 1473
VpaK11BI GGWCC 2 cut(s) 782, 1340
XapI RAATTY 2 cut(s) 362, 1292
XceI RCATGY 1 cut(s) 74
XmiI GTMKAC 1 cut(s) 834
ZrmI AGTACT 1 cut(s) 1216
Zsp2I ATGCAT 2 cut(s) 76, 770
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.