Rroxscaffold_2G00147880

Short calmodulin-binding motif containing conserved Ile and Gln residues.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000760
Physical Location & Seq
Reverse (-)
85592003 .. 85592899
897 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00147880.1

Sequence Viewer

Length: 519 bp
ATGTCGAGAAAAGCTCGAGCTAGGACAGCTCTACAGCGCCAATGCATGGAGTTCCTTGGACCGAAAGAAAGGAAATCCCATGAAGTAGTAGTGGAAAATGGCAAGCTAATGTACAAGCAAAGTAGGATGCTTGTGAATAGTAGTGAAGGTTCCAAATCGATATTCGTGCTAAGCCCAACAAGGGCTTTGTACGTTGGTCAGAAGAAGGGTCAGTTTGACCATTCGAGTTTTCTATCTGGAGGTGCTACCACAGCAGCTGGAAGATTGGTTGCCCATGATGGCGTTCTTGAGGCCATTTGGCCATACAGCGATCATTACCACCCAACTGAAGAAAACTCCATGGAATTCATCAGCTTCCTCCAAGATAACCATGTCGATCTCACTAATGTCAAGAGGTGTGCAATTGCTTATGACTATCCTTCAATGAAGAAAACTGAAGGGGAACTAACAAAGCAAAACTCATTTAAGTCGACGCAATCGGATGGGATGGCACAAACACAACCAATGTCAATGACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

172

Amino Acids

19.32

Weight (kDa)

9.06

Isoelectric Point (pI)

46.56

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 46
AccI GTMKAC 1 cut(s) 470
AcoI YGGCCR 1 cut(s) 299
AcsI RAATTY 1 cut(s) 344
AcuI CTGAAG 2 cut(s) 348, 456
AfaI GTAC 2 cut(s) 113, 191
AfiI CCNNNNNNNGG 2 cut(s) 46, 181
AgsI TTSAA 1 cut(s) 423
AjuI GAANNNNNNNTTGG 2 cut(s) 146, 178
AluBI AGCT 6 cut(s) 14, 20, 29, 106, 257, 354
AluI AGCT 6 cut(s) 14, 20, 29, 106, 257, 354
AlwNI CAGNNNCTG 1 cut(s) 257
Ama87I CYCGRG 1 cut(s) 15
AoxI GGCC 2 cut(s) 291, 299
ApeKI GCWGC 1 cut(s) 254
ApoI RAATTY 1 cut(s) 344
AspLEI GCGC 1 cut(s) 39
AspS9I GGNCC 1 cut(s) 59
AvaI CYCGRG 1 cut(s) 15
AvaII GGWCC 1 cut(s) 59
BalI TGGCCA 1 cut(s) 301
BbvI GCAGC 1 cut(s) 266
BccI CCATC 3 cut(s) 272, 476, 481
BcgI CGANNNNNNTGC 2 cut(s) 148, 182
BfaI CTAG 1 cut(s) 21
BfmI CTRYAG 1 cut(s) 32
BfoI RGCGCY 1 cut(s) 40
BisI GCNGC 1 cut(s) 255
BlpI GCTNAGC 1 cut(s) 170
BlsI GCNGC 1 cut(s) 256
Bme18I GGWCC 1 cut(s) 59
BmeT110I CYCGRG 1 cut(s) 15
BmgT120I GGNCC 1 cut(s) 59
BmiI GGNNCC 1 cut(s) 151
BmsI GCATC 1 cut(s) 117
BplI GAGNNNNNCTC 1 cut(s) 30
BpmI CTGGAG 1 cut(s) 258
Bpu1102I GCTNAGC 1 cut(s) 170
BpuEI CTTGAG 1 cut(s) 308
Bsa29I ATCGAT 1 cut(s) 158
BsaJI CCNNGG 2 cut(s) 55, 339
Bsc4I CCNNNNNNNGG 2 cut(s) 46, 181
BseCI ATCGAT 1 cut(s) 158
BseDI CCNNGG 2 cut(s) 55, 339
BseGI GGATG 3 cut(s) 132, 487, 492
BseLI CCNNNNNNNGG 2 cut(s) 46, 181
BseXI GCAGC 1 cut(s) 266
BshFI GGCC 2 cut(s) 293, 301
BshVI ATCGAT 1 cut(s) 158
BsiHKCI CYCGRG 1 cut(s) 15
BslI CCNNNNNNNGG 2 cut(s) 46, 181
BsnI GGCC 2 cut(s) 293, 301
BsoBI CYCGRG 1 cut(s) 15
Bsp1407I TGTACA 1 cut(s) 111
Bsp143I GATC 2 cut(s) 310, 376
Bsp1720I GCTNAGC 1 cut(s) 170
Bsp19I CCATGG 1 cut(s) 339
BspANI GGCC 2 cut(s) 293, 301
BspDI ATCGAT 1 cut(s) 158
BspLI GGNNCC 1 cut(s) 151
BsrGI TGTACA 1 cut(s) 111
BssECI CCNNGG 2 cut(s) 55, 339
BssMI GATC 2 cut(s) 310, 376
BssT1I CCWWGG 2 cut(s) 55, 339
BstAUI TGTACA 1 cut(s) 111
BstC8I GCNNGC 1 cut(s) 104
BstDEI CTNAG 1 cut(s) 170
BstDSI CCRYGG 1 cut(s) 339
BstF5I GGATG 3 cut(s) 132, 487, 492
BstH2I RGCGCY 1 cut(s) 40
BstHHI GCGC 1 cut(s) 39
BstKTI GATC 2 cut(s) 313, 379
BstMBI GATC 2 cut(s) 310, 376
BstMWI GCNNNNNNNGC 2 cut(s) 26, 251
BstSFI CTRYAG 1 cut(s) 32
BstV1I GCAGC 1 cut(s) 266
Bsu15I ATCGAT 1 cut(s) 158
BsuRI GGCC 2 cut(s) 293, 301
BsuTUI ATCGAT 1 cut(s) 158
BtgI CCRYGG 1 cut(s) 339
BtsCI GGATG 3 cut(s) 132, 487, 492
Cac8I GCNNGC 1 cut(s) 104
CaiI CAGNNNCTG 1 cut(s) 257
CfoI GCGC 1 cut(s) 39
Cfr13I GGNCC 1 cut(s) 59
ClaI ATCGAT 1 cut(s) 158
CseI GACGC 1 cut(s) 481
Csp6I GTAC 2 cut(s) 112, 190
CviAII CATG 5 cut(s) 46, 80, 275, 340, 371
CviQI GTAC 2 cut(s) 112, 190
DdeI CTNAG 1 cut(s) 170
DpnI GATC 2 cut(s) 312, 378
DpnII GATC 2 cut(s) 310, 376
EaeI YGGCCR 1 cut(s) 299
Eco130I CCWWGG 2 cut(s) 55, 339
Eco47I GGWCC 1 cut(s) 59
Eco57I CTGAAG 2 cut(s) 348, 456
Eco88I CYCGRG 1 cut(s) 15
EcoRI GAATTC 1 cut(s) 344
EcoT14I CCWWGG 2 cut(s) 55, 339
EcoT22I ATGCAT 1 cut(s) 47
ErhI CCWWGG 2 cut(s) 55, 339
FaeI CATG 5 cut(s) 49, 83, 278, 343, 374
FaiI YATR 7 cut(s) 47, 81, 276, 304, 341, 372, 411
FatI CATG 5 cut(s) 45, 79, 274, 339, 370
FblI GTMKAC 1 cut(s) 470
Fnu4HI GCNGC 1 cut(s) 255
FokI GGATG 3 cut(s) 139, 494, 499
Fsp4HI GCNGC 1 cut(s) 255
FspBI CTAG 1 cut(s) 21
GlaI GCGC 1 cut(s) 38
GluI GCNGC 1 cut(s) 255
GsuI CTGGAG 1 cut(s) 258
HaeII RGCGCY 1 cut(s) 40
HaeIII GGCC 2 cut(s) 293, 301
HgaI GACGC 1 cut(s) 481
HhaI GCGC 1 cut(s) 39
Hin1II CATG 5 cut(s) 49, 83, 278, 343, 374
Hin6I GCGC 1 cut(s) 37
HinP1I GCGC 1 cut(s) 37
HincII GTYRAC 1 cut(s) 471
HindII GTYRAC 1 cut(s) 471
Hpy166II GTNNAC 1 cut(s) 471
Hpy188I TCNGA 2 cut(s) 201, 481
Hpy188III TCNNGA 4 cut(s) 6, 237, 287, 391
Hpy8I GTNNAC 1 cut(s) 471
Hpy99I CGWCG 1 cut(s) 475
HpyAV CCTTC 4 cut(s) 140, 199, 429, 431
HpyCH4IV ACGT 1 cut(s) 192
HpyCH4V TGCA 2 cut(s) 45, 401
HpyF10VI GCNNNNNNNGC 2 cut(s) 26, 251
HpyF3I CTNAG 1 cut(s) 170
HpySE526I ACGT 1 cut(s) 192
Hsp92II CATG 5 cut(s) 49, 83, 278, 343, 374
HspAI GCGC 1 cut(s) 37
Kzo9I GATC 2 cut(s) 310, 376
LpnPI CCDG 2 cut(s) 222, 243
Lsp1109I GCAGC 1 cut(s) 266
LweI GCATC 1 cut(s) 117
MaeI CTAG 1 cut(s) 21
MaeII ACGT 1 cut(s) 192
MalI GATC 2 cut(s) 312, 378
MboI GATC 2 cut(s) 310, 376
MboII GAAGA 4 cut(s) 214, 273, 341, 439
MfeI CAATTG 1 cut(s) 402
MlsI TGGCCA 1 cut(s) 301
MluCI AATT 2 cut(s) 344, 402
MluNI TGGCCA 1 cut(s) 301
MnlI CCTC 4 cut(s) 233, 283, 368, 387
Mox20I TGGCCA 1 cut(s) 301
Mph1103I ATGCAT 1 cut(s) 47
MscI TGGCCA 1 cut(s) 301
MseI TTAA 1 cut(s) 465
Msp20I TGGCCA 1 cut(s) 301
MspA1I CMGCKG 1 cut(s) 257
MunI CAATTG 1 cut(s) 402
MwoI GCNNNNNNNGC 2 cut(s) 26, 251
NcoI CCATGG 1 cut(s) 339
NdeII GATC 2 cut(s) 310, 376
NlaIII CATG 5 cut(s) 49, 83, 278, 343, 374
NlaIV GGNNCC 1 cut(s) 151
NsiI ATGCAT 1 cut(s) 47
PaeR7I CTCGAG 1 cut(s) 15
PflMI CCANNNNNTGG 1 cut(s) 46
PkrI GCNGC 1 cut(s) 256
PspN4I GGNNCC 1 cut(s) 151
PspPI GGNCC 1 cut(s) 59
PspXI VCTCGAGB 1 cut(s) 15
PsrI GAACNNNNNNTAC 2 cut(s) 133, 165
PstNI CAGNNNCTG 1 cut(s) 257
PvuII CAGCTG 1 cut(s) 257
RsaI GTAC 2 cut(s) 113, 191
RsaNI GTAC 2 cut(s) 112, 190
SalI GTCGAC 1 cut(s) 469
SaqAI TTAA 1 cut(s) 465
SatI GCNGC 1 cut(s) 255
Sau3AI GATC 2 cut(s) 310, 376
Sau96I GGNCC 1 cut(s) 59
SfaNI GCATC 1 cut(s) 117
SfcI CTRYAG 1 cut(s) 32
Sfr274I CTCGAG 1 cut(s) 15
SinI GGWCC 1 cut(s) 59
SlaI CTCGAG 1 cut(s) 15
SmlI CTYRAG 2 cut(s) 15, 287
SmoI CTYRAG 2 cut(s) 15, 287
Sse9I AATT 2 cut(s) 344, 402
SspMI CTAG 1 cut(s) 21
StyI CCWWGG 2 cut(s) 55, 339
TaiI ACGT 1 cut(s) 195
TaqI TCGA 6 cut(s) 5, 16, 158, 224, 375, 470
TaqII GACCGA 1 cut(s) 76
TasI AATT 2 cut(s) 344, 402
TatI WGTACW 1 cut(s) 111
Tru1I TTAA 1 cut(s) 465
Tru9I TTAA 1 cut(s) 465
TseI GCWGC 1 cut(s) 254
TspDTI ATGAA 3 cut(s) 96, 337, 440
Van91I CCANNNNNTGG 1 cut(s) 46
VpaK11BI GGWCC 1 cut(s) 59
XapI RAATTY 1 cut(s) 344
XhoI CTCGAG 1 cut(s) 15
XmiI GTMKAC 1 cut(s) 470
XspI CTAG 1 cut(s) 21
Zsp2I ATGCAT 1 cut(s) 47
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.