Rorug02G0083600

Short calmodulin-binding motif containing conserved Ile and Gln residues.

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000760
Physical Location & Seq
Reverse (-)
6719427 .. 6721885
2459 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0083600.1

Sequence Viewer

Length: 924 bp
ATGGTGTGGTTTCAATGCGAGGACTGTGGAGACAATCTCAAGAAACCCAAGTTGCCTCACCACTTCAACACCTGCTCTGCTCGCAAGCTTTCGTGTATCGATTGTGGTGAGATGTTTGGGAGAGAAACTGTTCAGGGTCACACACAGTGTATTACTGAAGCGGAAAAATATGGTCCTAAGGGACAAGCGAAAGCTGTGAATGGTGGGGGAGCCAAGGCCAACAAGGATTCGAAGCAGCAAGCCGATTTTGATATAACTGTTGGGTTATCTGAGCGCCCGCCCTGGTTTTGTAGTCTTTGCAATACGAATGCGACCAGTAGGCAGACACTGATGCTCCATGCTGAAGGAAAGAAGCACAGGGGAAAGGCTCGAGCTATTCATGCTGCCAAGCAAAAGGCTATGCAGCCACAAGAATCTGCTCCAGATACAAAACCCCAACCTGAGGAAGCTCCAAAAGGAATAGAAGAACCTAAATTGCAAGATGCATCAGGAGTCGATACTGTCCATAAAATTTCTGAAGCAGAGAATGGAAATTTGCTGTCAAAGAAAAAGAGAAAACTAGAAGAGTCCAAGAATGATGAATCAAGAAAAAAGACTAAGGATACTACATCAGGGGAAGTAGGCAATGAGAAAGTGATTCAGAATGGGAAAGCAGAAGCAAATGGAAAAGAGACCAAGTTGAAGAAAGATACAGAAGACTCCGAGAAAAAAATTAAGTGGAAGAAGTTGATTACATCAACATTAAAATCGAAAGATGGAGTTCTGAAGATGAGAAAACTGAAGAAACTTGTTCTGAATGCTATTGCGGAATCTGGCATAACAGAAGATGAAGGAAAACTGAGTACGACCTTTGAGGATAAGGTCAATTCAAGCTCCAGATTTAGAGTTGAGAACAAGTATGTTCACTTGGTGGCCAAGGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

307

Amino Acids

34.04

Weight (kDa)

9.27

Isoelectric Point (pI)

28.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-LYAR PF08790 30 - 57 2.7e-14 LYAR-type C2HC zinc finger
WHD_LYAR PF25879 235 - 306 7e-25 LYAR family winged helix domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 80
Acc36I ACCTGC 1 cut(s) 80
AciI CCGC 3 cut(s) 161, 278, 806
AcoI YGGCCR 1 cut(s) 912
AcsI RAATTY 2 cut(s) 510, 532
AcuI CTGAAG 5 cut(s) 177, 363, 537, 785, 800
AdeI CACNNNGTG 2 cut(s) 147, 910
AfaI GTAC 1 cut(s) 844
AfiI CCNNNNNNNGG 1 cut(s) 442
AgsI TTSAA 4 cut(s) 14, 67, 682, 870
AjnI CCWGG 1 cut(s) 281
AluBI AGCT 5 cut(s) 88, 194, 374, 449, 873
AluI AGCT 5 cut(s) 88, 194, 374, 449, 873
Alw26I GTCTC 2 cut(s) 24, 665
AlwNI CAGNNNCTG 1 cut(s) 328
Ama87I CYCGRG 1 cut(s) 369
AoxI GGCC 2 cut(s) 216, 912
ApeKI GCWGC 3 cut(s) 235, 383, 403
ApoI RAATTY 2 cut(s) 510, 532
Asp700I GAANNNNTTC 1 cut(s) 129
AspLEI GCGC 1 cut(s) 276
AspS9I GGNCC 1 cut(s) 173
AsuHPI GGTGA 2 cut(s) 50, 119
AsuII TTCGAA 1 cut(s) 230
AvaI CYCGRG 1 cut(s) 369
AvaII GGWCC 1 cut(s) 173
AxyI CCTNAGG 2 cut(s) 177, 441
BalI TGGCCA 1 cut(s) 914
BbsI GAAGAC 1 cut(s) 702
BbvI GCAGC 3 cut(s) 247, 370, 415
BccI CCATC 1 cut(s) 749
BciT130I CCWGG 1 cut(s) 283
BciVI GTATCC 1 cut(s) 595
BcoDI GTCTC 2 cut(s) 24, 665
BfaI CTAG 1 cut(s) 560
BfoI RGCGCY 1 cut(s) 277
BfuAI ACCTGC 1 cut(s) 80
BfuI GTATCC 1 cut(s) 595
BisI GCNGC 3 cut(s) 236, 384, 404
BlsI GCNGC 3 cut(s) 237, 385, 405
Bme1390I CCNGG 1 cut(s) 283
Bme18I GGWCC 1 cut(s) 173
BmeT110I CYCGRG 1 cut(s) 369
BmgT120I GGNCC 1 cut(s) 173
BmiI GGNNCC 1 cut(s) 211
BmrFI CCNGG 1 cut(s) 283
BmsI GCATC 3 cut(s) 321, 472, 494
BpiI GAAGAC 1 cut(s) 702
BplI GAGNNNNNCTC 2 cut(s) 21, 53
BpmI CTGGAG 2 cut(s) 405, 859
Bpu14I TTCGAA 1 cut(s) 230
BpuEI CTTGAG 1 cut(s) 23
Bsa29I ATCGAT 1 cut(s) 99
BsaI GGTCTC 1 cut(s) 665
BsaJI CCNNGG 3 cut(s) 213, 281, 915
BsaXI ACNNNNNCTCC 2 cut(s) 318, 348
Bsc4I CCNNNNNNNGG 1 cut(s) 442
Bse1I ACTGG 1 cut(s) 315
Bse21I CCTNAGG 2 cut(s) 177, 441
Bse3DI GCAATG 1 cut(s) 631
BseBI CCWGG 1 cut(s) 283
BseCI ATCGAT 1 cut(s) 99
BseDI CCNNGG 3 cut(s) 213, 281, 915
BseLI CCNNNNNNNGG 1 cut(s) 442
BseMI GCAATG 1 cut(s) 631
BseMII CTCAG 3 cut(s) 261, 432, 830
BseNI ACTGG 1 cut(s) 315
BseXI GCAGC 3 cut(s) 247, 370, 415
BshFI GGCC 2 cut(s) 218, 914
BshVI ATCGAT 1 cut(s) 99
BsiHKCI CYCGRG 1 cut(s) 369
BslFI GGGAC 1 cut(s) 195
BslI CCNNNNNNNGG 1 cut(s) 442
BsmAI GTCTC 2 cut(s) 24, 665
BsmFI GGGAC 1 cut(s) 195
BsmI GAATGC 2 cut(s) 313, 802
BsnI GGCC 2 cut(s) 218, 914
Bso31I GGTCTC 1 cut(s) 665
BsoBI CYCGRG 1 cut(s) 369
Bsp119I TTCGAA 1 cut(s) 230
BspACI CCGC 3 cut(s) 161, 278, 806
BspANI GGCC 2 cut(s) 218, 914
BspCNI CTCAG 3 cut(s) 262, 433, 831
BspDI ATCGAT 1 cut(s) 99
BspLI GGNNCC 1 cut(s) 211
BspMI ACCTGC 1 cut(s) 80
BspT104I TTCGAA 1 cut(s) 230
BspTNI GGTCTC 1 cut(s) 665
BsrDI GCAATG 1 cut(s) 631
BsrI ACTGG 1 cut(s) 315
BssECI CCNNGG 3 cut(s) 213, 281, 915
BssT1I CCWWGG 2 cut(s) 213, 915
Bst2UI CCWGG 1 cut(s) 283
Bst4CI ACNGT 5 cut(s) 26, 130, 147, 259, 502
Bst6I CTCTTC 1 cut(s) 558
BstBI TTCGAA 1 cut(s) 230
BstC8I GCNNGC 4 cut(s) 82, 86, 240, 278
BstDEI CTNAG 5 cut(s) 177, 270, 441, 597, 839
BstH2I RGCGCY 1 cut(s) 277
BstHHI GCGC 1 cut(s) 276
BstMAI GTCTC 2 cut(s) 24, 665
BstMWI GCNNNNNNNGC 2 cut(s) 81, 380
BstNI CCWGG 1 cut(s) 283
BstSCI CCNGG 1 cut(s) 281
BstV1I GCAGC 3 cut(s) 247, 370, 415
BstV2I GAAGAC 1 cut(s) 702
Bsu15I ATCGAT 1 cut(s) 99
Bsu36I CCTNAGG 2 cut(s) 177, 441
BsuI GTATCC 1 cut(s) 595
BsuRI GGCC 2 cut(s) 218, 914
BsuTUI ATCGAT 1 cut(s) 99
BtsIMutI CAGTG 2 cut(s) 152, 326
BveI ACCTGC 1 cut(s) 80
Cac8I GCNNGC 4 cut(s) 82, 86, 240, 278
CaiI CAGNNNCTG 1 cut(s) 328
CfoI GCGC 1 cut(s) 276
Cfr13I GGNCC 1 cut(s) 173
ClaI ATCGAT 1 cut(s) 99
Csp6I GTAC 1 cut(s) 843
CviAII CATG 2 cut(s) 338, 380
CviQI GTAC 1 cut(s) 843
DdeI CTNAG 5 cut(s) 177, 270, 441, 597, 839
DraIII CACNNNGTG 2 cut(s) 147, 910
EaeI YGGCCR 1 cut(s) 912
Eam1104I CTCTTC 1 cut(s) 558
EarI CTCTTC 1 cut(s) 558
Eco130I CCWWGG 2 cut(s) 213, 915
Eco31I GGTCTC 1 cut(s) 665
Eco47I GGWCC 1 cut(s) 173
Eco57I CTGAAG 5 cut(s) 177, 363, 537, 785, 800
Eco81I CCTNAGG 2 cut(s) 177, 441
Eco88I CYCGRG 1 cut(s) 369
EcoRII CCWGG 1 cut(s) 281
EcoT14I CCWWGG 2 cut(s) 213, 915
EcoT22I ATGCAT 1 cut(s) 487
ErhI CCWWGG 2 cut(s) 213, 915
FaeI CATG 2 cut(s) 341, 383
FaiI YATR 8 cut(s) 171, 254, 339, 381, 401, 507, 818, 900
FaqI GGGAC 1 cut(s) 195
FatI CATG 2 cut(s) 337, 379
FauI CCCGC 1 cut(s) 285
Fnu4HI GCNGC 3 cut(s) 236, 384, 404
Fsp4HI GCNGC 3 cut(s) 236, 384, 404
FspBI CTAG 1 cut(s) 560
GlaI GCGC 1 cut(s) 275
GluI GCNGC 3 cut(s) 236, 384, 404
GsuI CTGGAG 2 cut(s) 405, 859
HaeII RGCGCY 1 cut(s) 277
HaeIII GGCC 2 cut(s) 218, 914
HhaI GCGC 1 cut(s) 276
Hin1II CATG 2 cut(s) 341, 383
Hin6I GCGC 1 cut(s) 274
HinP1I GCGC 1 cut(s) 274
HindIII AAGCTT 1 cut(s) 86
HinfI GANTC 8 cut(s) 227, 413, 492, 566, 581, 637, 698, 809
HphI GGTGA 2 cut(s) 50, 119
Hpy166II GTNNAC 1 cut(s) 904
Hpy188I TCNGA 6 cut(s) 271, 517, 642, 703, 765, 795
Hpy188III TCNNGA 5 cut(s) 40, 422, 489, 585, 876
Hpy8I GTNNAC 1 cut(s) 904
HpyAV CCTTC 2 cut(s) 338, 824
HpyCH4III ACNGT 5 cut(s) 26, 130, 147, 259, 502
HpyCH4V TGCA 4 cut(s) 300, 403, 478, 485
HpyF10VI GCNNNNNNNGC 2 cut(s) 81, 380
HpyF3I CTNAG 5 cut(s) 177, 270, 441, 597, 839
Hsp92II CATG 2 cut(s) 341, 383
HspAI GCGC 1 cut(s) 274
LmnI GCTCC 5 cut(s) 209, 339, 424, 454, 878
Lsp1109I GCAGC 3 cut(s) 247, 370, 415
LweI GCATC 3 cut(s) 321, 472, 494
MaeI CTAG 1 cut(s) 560
MaeIII GTNAC 1 cut(s) 137
MboII GAAGA 8 cut(s) 476, 575, 694, 707, 733, 778, 793, 836
MlsI TGGCCA 1 cut(s) 914
MluCI AATT 5 cut(s) 473, 510, 532, 711, 865
MluNI TGGCCA 1 cut(s) 914
MlyI GAGTC 3 cut(s) 501, 575, 692
MnlI CCTC 4 cut(s) 13, 66, 436, 847
Mox20I TGGCCA 1 cut(s) 914
Mph1103I ATGCAT 1 cut(s) 487
MroXI GAANNNNTTC 1 cut(s) 129
MscI TGGCCA 1 cut(s) 914
MseI TTAA 3 cut(s) 714, 743, 922
Msp20I TGGCCA 1 cut(s) 914
MspR9I CCNGG 1 cut(s) 283
Mva1269I GAATGC 2 cut(s) 313, 802
MvaI CCWGG 1 cut(s) 283
MwoI GCNNNNNNNGC 2 cut(s) 81, 380
NlaIII CATG 2 cut(s) 341, 383
NlaIV GGNNCC 1 cut(s) 211
NmuCI GTSAC 1 cut(s) 137
NsiI ATGCAT 1 cut(s) 487
NspV TTCGAA 1 cut(s) 230
PaeR7I CTCGAG 1 cut(s) 369
PaqCI CACCTGC 1 cut(s) 80
PctI GAATGC 2 cut(s) 313, 802
PdmI GAANNNNTTC 1 cut(s) 129
PfeI GAWTC 5 cut(s) 227, 413, 581, 637, 809
PkrI GCNGC 3 cut(s) 237, 385, 405
PleI GAGTC 3 cut(s) 500, 574, 692
PpsI GAGTC 3 cut(s) 500, 574, 692
Psp6I CCWGG 1 cut(s) 281
PspGI CCWGG 1 cut(s) 281
PspN4I GGNNCC 1 cut(s) 211
PspPI GGNCC 1 cut(s) 173
PspXI VCTCGAGB 1 cut(s) 369
PstNI CAGNNNCTG 1 cut(s) 328
RsaI GTAC 1 cut(s) 844
RsaNI GTAC 1 cut(s) 843
SaqAI TTAA 3 cut(s) 714, 743, 922
SatI GCNGC 3 cut(s) 236, 384, 404
Sau96I GGNCC 1 cut(s) 173
SchI GAGTC 3 cut(s) 501, 575, 692
ScrFI CCNGG 1 cut(s) 283
SfaNI GCATC 3 cut(s) 321, 472, 494
Sfr274I CTCGAG 1 cut(s) 369
SfuI TTCGAA 1 cut(s) 230
SinI GGWCC 1 cut(s) 173
SlaI CTCGAG 1 cut(s) 369
SmlI CTYRAG 2 cut(s) 38, 369
SmoI CTYRAG 2 cut(s) 38, 369
Sse9I AATT 5 cut(s) 473, 510, 532, 711, 865
SsiI CCGC 3 cut(s) 161, 278, 806
SspMI CTAG 1 cut(s) 560
StyD4I CCNGG 1 cut(s) 281
StyI CCWWGG 2 cut(s) 213, 915
TaaI ACNGT 5 cut(s) 26, 130, 147, 259, 502
TaqI TCGA 5 cut(s) 99, 230, 370, 495, 749
TasI AATT 5 cut(s) 473, 510, 532, 711, 865
TfiI GAWTC 5 cut(s) 227, 413, 581, 637, 809
Tru1I TTAA 3 cut(s) 714, 743, 922
Tru9I TTAA 3 cut(s) 714, 743, 922
TscAI CASTG 2 cut(s) 152, 333
TseFI GTSAC 1 cut(s) 137
TseI GCWGC 3 cut(s) 235, 383, 403
Tsp45I GTSAC 1 cut(s) 137
TspDTI ATGAA 3 cut(s) 368, 594, 843
TspRI CASTG 2 cut(s) 152, 333
VpaK11BI GGWCC 1 cut(s) 173
XapI RAATTY 2 cut(s) 510, 532
XhoI CTCGAG 1 cut(s) 369
XmnI GAANNNNTTC 1 cut(s) 129
XspI CTAG 1 cut(s) 560
Zsp2I ATGCAT 1 cut(s) 487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.