FvH4_3g39340

Leucine-rich repeats, typical (most populated) subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000886
Physical Location & Seq
Forward (+)
33480422 .. 33482541
2120 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g39340.t1

Sequence Viewer

Length: 1023 bp
ATGTCGAAGGACGGCGACGACGTCGTTTTGGAGCAGGAGCGAAGAAGATGGGAGAAGATGGACGTGGATTGCTTAGCAAATGCGTTTAGCAGAGCTGGAATGGAGTCGCTGCTTTTGGCTCTCCCTTTTGTCTGTAAGTCGTGGTACAAAGCTAGCCTCAACCCTATATGCTGGGCATGTCTTTCCTTTCCAGATTATATACCTTCTCCCTTGTTGGTTAATACTGTTGAAGAAATTGAAATGGGTCCTCAGAGTTTCGGTTCATTTTATGATAAGTTTGTGGATGAATATGGAATCGATAAGAGTCGGTTTTCGATTACTGCGTTTGTAAAGTTGGTGGTTGATCGCAGCAAAGGGAAGGCTACTCAACTCGAGCTACCTATGTATAGTACAGAAGCAGCTCTGAGATATGTTTCAGATGCGTGTCCTCTGCTCAAACACTTAACGTTTGACGATGATCTGGTGATTTTCAAGCATTCTCGAATTCTTCCCGAAGTGATTGGAAAGTGGAAGTTTTTAGAGGGCTTGTCTTTGCGGCACAGCTTCATGGTCAATGACCAGCAAGGAAGACGTGGGAAATACTTGAGTAGATATTTTCGGGAGTTGTTGGCATTTCCGAGCTGCTTTGATGTTTTCGAGAGAATTGTAGCGCAGATCGGCACTCACTGCAAGCATTTCAGGCAGCTAACTCTTATGAAAACCCGTCTTCATCGAGATGAGGCGTTGACAATTGTGAACTTTCTGCCACACCTTCAGACCATGTCTGTGACCGGGTGCATCATCGAAAGGGATAGTATTCTCACAATAGTTCGGGGATGCAAAGAGCTTCGGGTTTTTTGTGCCAAGGAATGTACAGGCTTTGAAGAGGGTGGTGAAGAATTATACAAGCTTGCTCGCCAGACTGGTAAGCTTGTATGTGTGGACCGTGTACGCCGAGCTGAACGTCTGATTCTGGGGGTTTTGAGCACTACTTTAATGAGAGAATCTGTATACAACAGGTTGCGTATATGCCTGGGTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRR_8 ubiquitin

Protein Analysis

341

Amino Acids

39.14

Weight (kDa)

8.5

Isoelectric Point (pI)

51.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 24
AccI GTMKAC 1 cut(s) 990
AciI CCGC 1 cut(s) 535
AclI AACGTT 1 cut(s) 446
AcsI RAATTY 1 cut(s) 483
AcuI CTGAAG 1 cut(s) 737
AcyI GRCGYC 1 cut(s) 21
AfaI GTAC 4 cut(s) 146, 391, 853, 930
AgsI TTSAA 4 cut(s) 230, 239, 472, 863
AjiI CACGTC 2 cut(s) 64, 572
AjnI CCWGG 1 cut(s) 1011
Alw21I GWGCWC 1 cut(s) 968
Ama87I CYCGRG 1 cut(s) 371
ApeKI GCWGC 5 cut(s) 109, 348, 398, 621, 682
ApoI RAATTY 1 cut(s) 483
AspLEI GCGC 1 cut(s) 652
AspS9I GGNCC 2 cut(s) 245, 922
AsuC2I CCSGG 1 cut(s) 772
AsuHPI GGTGA 2 cut(s) 475, 884
AsuNHI GCTAGC 1 cut(s) 152
AvaI CYCGRG 1 cut(s) 371
AvaII GGWCC 2 cut(s) 245, 922
BbsI GAAGAC 2 cut(s) 574, 698
Bbv12I GWGCWC 1 cut(s) 968
BbvI GCAGC 5 cut(s) 96, 360, 410, 608, 694
BccI CCATC 2 cut(s) 42, 52
BceAI ACGGC 1 cut(s) 28
BciT130I CCWGG 1 cut(s) 1013
BcnI CCSGG 1 cut(s) 772
BfaI CTAG 1 cut(s) 153
BisI GCNGC 6 cut(s) 110, 349, 399, 536, 622, 683
BlpI GCTNAGC 1 cut(s) 73
BlsI GCNGC 6 cut(s) 111, 350, 400, 537, 623, 684
Bme1390I CCNGG 2 cut(s) 772, 1013
Bme18I GGWCC 2 cut(s) 245, 922
BmeT110I CYCGRG 1 cut(s) 371
BmgBI CACGTC 2 cut(s) 64, 572
BmgT120I GGNCC 2 cut(s) 245, 922
BmiI GGNNCC 1 cut(s) 246
BmrFI CCNGG 2 cut(s) 772, 1013
BmsI GCATC 3 cut(s) 409, 786, 806
BmtI GCTAGC 1 cut(s) 156
BpiI GAAGAC 2 cut(s) 574, 698
Bpu1102I GCTNAGC 1 cut(s) 73
BpuEI CTTGAG 1 cut(s) 604
BpuMI CCSGG 1 cut(s) 772
Bsa29I ATCGAT 1 cut(s) 297
BsaHI GRCGYC 1 cut(s) 21
BsaJI CCNNGG 2 cut(s) 843, 1012
Bse1I ACTGG 1 cut(s) 907
BseBI CCWGG 1 cut(s) 1013
BseCI ATCGAT 1 cut(s) 297
BseDI CCNNGG 2 cut(s) 843, 1012
BseGI GGATG 2 cut(s) 289, 821
BseMII CTCAG 2 cut(s) 263, 395
BseNI ACTGG 1 cut(s) 907
BseXI GCAGC 5 cut(s) 96, 360, 410, 608, 694
BseYI CCCAGC 1 cut(s) 171
BshVI ATCGAT 1 cut(s) 297
BsiHKAI GWGCWC 1 cut(s) 968
BsiHKCI CYCGRG 1 cut(s) 371
BsiSI CCGG 1 cut(s) 771
BsmI GAATGC 1 cut(s) 475
BsoBI CYCGRG 1 cut(s) 371
Bsp1286I GDGCHC 1 cut(s) 968
Bsp1407I TGTACA 1 cut(s) 851
Bsp143I GATC 3 cut(s) 343, 457, 654
Bsp1720I GCTNAGC 1 cut(s) 73
BspACI CCGC 1 cut(s) 535
BspCNI CTCAG 2 cut(s) 262, 396
BspDI ATCGAT 1 cut(s) 297
BspLI GGNNCC 1 cut(s) 246
BspOI GCTAGC 1 cut(s) 156
BsrGI TGTACA 1 cut(s) 851
BsrI ACTGG 1 cut(s) 907
BssECI CCNNGG 2 cut(s) 843, 1012
BssMI GATC 3 cut(s) 343, 457, 654
BssNAI GTATAC 1 cut(s) 991
BssNI GRCGYC 1 cut(s) 21
BssT1I CCWWGG 1 cut(s) 843
Bst1107I GTATAC 1 cut(s) 991
Bst2UI CCWGG 1 cut(s) 1013
Bst4CI ACNGT 2 cut(s) 226, 926
Bst6I CTCTTC 1 cut(s) 858
BstACI GRCGYC 1 cut(s) 21
BstAPI GCANNNNNTGC 1 cut(s) 666
BstAUI TGTACA 1 cut(s) 851
BstC8I GCNNGC 4 cut(s) 154, 671, 891, 895
BstDEI CTNAG 3 cut(s) 73, 249, 404
BstF5I GGATG 2 cut(s) 289, 821
BstHHI GCGC 1 cut(s) 652
BstKTI GATC 3 cut(s) 346, 460, 657
BstMBI GATC 3 cut(s) 343, 457, 654
BstMWI GCNNNNNNNGC 2 cut(s) 666, 679
BstNI CCWGG 1 cut(s) 1013
BstNSI RCATGY 1 cut(s) 180
BstSCI CCNGG 2 cut(s) 770, 1011
BstV1I GCAGC 5 cut(s) 96, 360, 410, 608, 694
BstV2I GAAGAC 2 cut(s) 574, 698
BstZ17I GTATAC 1 cut(s) 991
Bsu15I ATCGAT 1 cut(s) 297
BsuTUI ATCGAT 1 cut(s) 297
BtrI CACGTC 2 cut(s) 64, 572
BtsCI GGATG 2 cut(s) 289, 821
BtsI GCAGTG 1 cut(s) 664
BtsIMutI CAGTG 1 cut(s) 664
Cac8I GCNNGC 4 cut(s) 154, 671, 891, 895
CfoI GCGC 1 cut(s) 652
Cfr13I GGNCC 2 cut(s) 245, 922
ClaI ATCGAT 1 cut(s) 297
Csp6I GTAC 4 cut(s) 145, 390, 852, 929
CviAII CATG 3 cut(s) 177, 547, 760
CviQI GTAC 4 cut(s) 145, 390, 852, 929
DdeI CTNAG 3 cut(s) 73, 249, 404
DpnI GATC 3 cut(s) 345, 459, 656
DpnII GATC 3 cut(s) 343, 457, 654
Eam1104I CTCTTC 1 cut(s) 858
EarI CTCTTC 1 cut(s) 858
Eco130I CCWWGG 1 cut(s) 843
Eco47I GGWCC 2 cut(s) 245, 922
Eco57I CTGAAG 1 cut(s) 737
Eco88I CYCGRG 1 cut(s) 371
EcoO109I RGGNCCY 1 cut(s) 245
EcoRI GAATTC 1 cut(s) 483
EcoRII CCWGG 1 cut(s) 1011
EcoT14I CCWWGG 1 cut(s) 843
ErhI CCWWGG 1 cut(s) 843
FaeI CATG 3 cut(s) 180, 550, 763
FatI CATG 3 cut(s) 176, 546, 759
FblI GTMKAC 1 cut(s) 990
Fnu4HI GCNGC 6 cut(s) 110, 349, 399, 536, 622, 683
FokI GGATG 2 cut(s) 296, 828
Fsp4HI GCNGC 6 cut(s) 110, 349, 399, 536, 622, 683
FspBI CTAG 1 cut(s) 153
GlaI GCGC 1 cut(s) 651
GluI GCNGC 6 cut(s) 110, 349, 399, 536, 622, 683
GsaI CCCAGC 1 cut(s) 175
HapII CCGG 1 cut(s) 771
HhaI GCGC 1 cut(s) 652
Hin1I GRCGYC 1 cut(s) 21
Hin1II CATG 3 cut(s) 180, 550, 763
Hin6I GCGC 1 cut(s) 650
HinP1I GCGC 1 cut(s) 650
HincII GTYRAC 1 cut(s) 726
HindII GTYRAC 1 cut(s) 726
HindIII AAGCTT 2 cut(s) 887, 908
HinfI GANTC 5 cut(s) 104, 294, 304, 949, 983
HpaII CCGG 1 cut(s) 771
HphI GGTGA 2 cut(s) 475, 884
Hpy166II GTNNAC 5 cut(s) 726, 736, 922, 929, 991
Hpy188I TCNGA 6 cut(s) 252, 405, 418, 618, 756, 948
Hpy188III TCNNGA 6 cut(s) 191, 480, 491, 599, 637, 713
Hpy8I GTNNAC 5 cut(s) 726, 736, 922, 929, 991
Hpy99I CGWCG 3 cut(s) 20, 23, 26
HpyAV CCTTC 3 cut(s) 213, 352, 761
HpyCH4III ACNGT 2 cut(s) 226, 926
HpyCH4IV ACGT 5 cut(s) 21, 63, 446, 571, 943
HpyCH4V TGCA 3 cut(s) 669, 777, 819
HpyF10VI GCNNNNNNNGC 2 cut(s) 666, 679
HpyF3I CTNAG 3 cut(s) 73, 249, 404
HpySE526I ACGT 5 cut(s) 21, 63, 446, 571, 943
Hsp92I GRCGYC 1 cut(s) 21
Hsp92II CATG 3 cut(s) 180, 550, 763
HspAI GCGC 1 cut(s) 650
Kzo9I GATC 3 cut(s) 343, 457, 654
LmnI GCTCC 2 cut(s) 31, 37
Lsp1109I GCAGC 5 cut(s) 96, 360, 410, 608, 694
LweI GCATC 3 cut(s) 409, 786, 806
MaeI CTAG 1 cut(s) 153
MaeII ACGT 5 cut(s) 21, 63, 446, 571, 943
MaeIII GTNAC 1 cut(s) 766
MalI GATC 3 cut(s) 345, 459, 656
MboI GATC 3 cut(s) 343, 457, 654
MboII GAAGA 9 cut(s) 54, 57, 67, 242, 479, 579, 698, 875, 887
MfeI CAATTG 1 cut(s) 729
MhlI GDGCHC 1 cut(s) 968
MluCI AATT 5 cut(s) 234, 483, 642, 729, 878
MlyI GAGTC 2 cut(s) 113, 313
MnlI CCTC 6 cut(s) 167, 258, 438, 514, 712, 859
MseI TTAA 3 cut(s) 219, 443, 974
MslI CAYNNNNRTG 2 cut(s) 714, 764
MspI CCGG 1 cut(s) 771
MspR9I CCNGG 2 cut(s) 772, 1013
MunI CAATTG 1 cut(s) 729
Mva1269I GAATGC 1 cut(s) 475
MvaI CCWGG 1 cut(s) 1013
MwoI GCNNNNNNNGC 2 cut(s) 666, 679
NciI CCSGG 1 cut(s) 772
NdeII GATC 3 cut(s) 343, 457, 654
NheI GCTAGC 1 cut(s) 152
NlaIII CATG 3 cut(s) 180, 550, 763
NlaIV GGNNCC 1 cut(s) 246
NmeAIII GCCGAG 1 cut(s) 959
NmuCI GTSAC 1 cut(s) 766
NspI RCATGY 1 cut(s) 180
PaeR7I CTCGAG 1 cut(s) 371
PcsI WCGNNNNNNNCGW 2 cut(s) 18, 320
PctI GAATGC 1 cut(s) 475
PfeI GAWTC 3 cut(s) 294, 949, 983
PflFI GACNNNGTC 2 cut(s) 20, 760
PkrI GCNGC 6 cut(s) 111, 350, 400, 537, 623, 684
PleI GAGTC 2 cut(s) 112, 312
PpsI GAGTC 2 cut(s) 112, 312
PpuMI RGGWCCY 1 cut(s) 245
Psp1406I AACGTT 1 cut(s) 446
Psp5II RGGWCCY 1 cut(s) 245
Psp6I CCWGG 1 cut(s) 1011
PspFI CCCAGC 1 cut(s) 171
PspGI CCWGG 1 cut(s) 1011
PspN4I GGNNCC 1 cut(s) 246
PspPI GGNCC 2 cut(s) 245, 922
PspPPI RGGWCCY 1 cut(s) 245
PspXI VCTCGAGB 1 cut(s) 371
PsyI GACNNNGTC 2 cut(s) 20, 760
RsaI GTAC 4 cut(s) 146, 391, 853, 930
RsaNI GTAC 4 cut(s) 145, 390, 852, 929
RseI CAYNNNNRTG 2 cut(s) 714, 764
SaqAI TTAA 3 cut(s) 219, 443, 974
SatI GCNGC 6 cut(s) 110, 349, 399, 536, 622, 683
Sau3AI GATC 3 cut(s) 343, 457, 654
Sau96I GGNCC 2 cut(s) 245, 922
SchI GAGTC 2 cut(s) 113, 313
ScrFI CCNGG 2 cut(s) 772, 1013
SduI GDGCHC 1 cut(s) 968
SfaNI GCATC 3 cut(s) 409, 786, 806
Sfr274I CTCGAG 1 cut(s) 371
SinI GGWCC 2 cut(s) 245, 922
SlaI CTCGAG 1 cut(s) 371
SmiMI CAYNNNNRTG 2 cut(s) 714, 764
SmlI CTYRAG 2 cut(s) 371, 583
SmoI CTYRAG 2 cut(s) 371, 583
Sse9I AATT 5 cut(s) 234, 483, 642, 729, 878
SsiI CCGC 1 cut(s) 535
SspMI CTAG 1 cut(s) 153
StyD4I CCNGG 2 cut(s) 770, 1011
StyI CCWWGG 1 cut(s) 843
TaaI ACNGT 2 cut(s) 226, 926
TaiI ACGT 5 cut(s) 24, 66, 449, 574, 946
TaqI TCGA 8 cut(s) 5, 297, 314, 372, 481, 636, 712, 783
TasI AATT 5 cut(s) 234, 483, 642, 729, 878
TatI WGTACW 2 cut(s) 389, 851
TauI GCSGC 1 cut(s) 538
TfiI GAWTC 3 cut(s) 294, 949, 983
Tru1I TTAA 3 cut(s) 219, 443, 974
Tru9I TTAA 3 cut(s) 219, 443, 974
TscAI CASTG 1 cut(s) 671
TseFI GTSAC 1 cut(s) 766
TseI GCWGC 5 cut(s) 109, 348, 398, 621, 682
Tsp45I GTSAC 1 cut(s) 766
TspDTI ATGAA 5 cut(s) 252, 300, 535, 698, 710
TspRI CASTG 1 cut(s) 671
Tth111I GACNNNGTC 2 cut(s) 20, 760
VpaK11BI GGWCC 2 cut(s) 245, 922
XapI RAATTY 1 cut(s) 483
XceI RCATGY 1 cut(s) 180
XhoI CTCGAG 1 cut(s) 371
XmiI GTMKAC 1 cut(s) 990
XspI CTAG 1 cut(s) 153
ZraI GACGTC 1 cut(s) 22
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.