Rh3BG239000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0000886
Physical Location & Seq
Reverse (-)
22494659 .. 22495642
984 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG239000.1

Sequence Viewer

Length: 984 bp
ATGGAAGATGAGGGAATCAGAATCAAAAGATGGAAGGATTTAGAGACGGACTGTTTGACTCGTGTGCTTTCCAGAGTTGGAATGGAGTCTTTGCTCCTGACAGTTCCTTTCGTCTGCAAATCATGGTACGTTACAAGTCTCAATCCCTTGTGCTTTCAAAGCCTCATATTTCCAGACTTTATGCCTTTTCCTCTGTTCACGTATTCTAATAATAGTAAGGAACCTCCTCGGAGTTTTGGTCCCTTTTATGATAAGTTTATAGACGAGTATGGCATTGACAAGTCTCGTTTCTCGATCACTGCATTTGTGAAGTTTGTAGTCAATCGTAGCAATGGATTAGCTATTCAACTTAAGCTACCAGAATTCTGTACCAAAGAAGGATTGATGTTTGTTTCGGACGCATGCCCTCTTCTTAAGAATGTATGGTTACCAGATGATTTGGTGATTTTCAAGACTTCTCACATTGTTTCACAGTTGATTAGGAAGTGGAAATTTTTGGAGAGCTTGATTTTGGGGTTTGGGATGTTGAACATCATGGACCAGTATGAAATGGATGGTAGACCTAAGTTTTTGAGTGAACATTTCAATAAGTTATTGACAATGAAAGATTTCTTCTCCCCTAATGCTTTGGATGCAATTTTAGTCCAGATTGGCACTCATTGCAAGCATTTTACGGATTTAATGGTTTATCATACTAGTCTTGGTGAAGTTGAGGCCTCAAGGATTGTGAAGTGTCATTGTCGAATTAATCGGGACAGTGTTGTTACTGTACTGAGGGGGTGCAAAGAGCTTGTACTTTTTATTGCCAATCAATGTGAAGGCTTTGAAGGGAGTGATGAAGAAATATCAAAATTTGGTTCTCAGATTATGAACTTTTCGTGCCAGGATTGTGTAGGCGGTGATTTGTTATTGACTTTTTTCAAACTTACTAATGTACATAGGAGGTTAGTTTCCATGATGTTCTTAATGTACATGGGAAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

327

Amino Acids

37.67

Weight (kDa)

6.66

Isoelectric Point (pI)

40.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 559
AciI CCGC 1 cut(s) 897
AcsI RAATTY 3 cut(s) 362, 491, 851
AfaI GTAC 6 cut(s) 128, 370, 771, 795, 936, 971
AflII CTTAAG 2 cut(s) 350, 413
AgsI TTSAA 7 cut(s) 158, 347, 451, 529, 586, 827, 922
AhlI ACTAGT 1 cut(s) 695
AjnI CCWGG 1 cut(s) 882
AluBI AGCT 4 cut(s) 341, 355, 504, 790
AluI AGCT 4 cut(s) 341, 355, 504, 790
Alw26I GTCTC 3 cut(s) 38, 143, 288
AoxI GGCC 1 cut(s) 714
ApoI RAATTY 3 cut(s) 362, 491, 851
ArsI GACNNNNNNTTYG 2 cut(s) 37, 69
AseI ATTAAT 1 cut(s) 747
Asp700I GAANNNNTTC 1 cut(s) 608
AspS9I GGNCC 2 cut(s) 239, 538
AsuHPI GGTGA 3 cut(s) 454, 716, 911
AvaII GGWCC 2 cut(s) 239, 538
BauI CACGAG 1 cut(s) 60
BccI CCATC 2 cut(s) 24, 548
BciT130I CCWGG 1 cut(s) 884
BcoDI GTCTC 3 cut(s) 38, 143, 288
BcuI ACTAGT 1 cut(s) 695
BfaI CTAG 1 cut(s) 696
BfrI CTTAAG 2 cut(s) 350, 413
Bme1390I CCNGG 1 cut(s) 884
Bme18I GGWCC 2 cut(s) 239, 538
BmgT120I GGNCC 2 cut(s) 239, 538
BmiI GGNNCC 2 cut(s) 222, 241
BmrFI CCNGG 1 cut(s) 884
BmsI GCATC 1 cut(s) 622
BpuEI CTTGAG 1 cut(s) 703
BsaAI YACGTR 1 cut(s) 201
BsaJI CCNNGG 1 cut(s) 227
BsaXI ACNNNNNCTCC 2 cut(s) 223, 253
Bse1I ACTGG 1 cut(s) 541
Bse3DI GCAATG 2 cut(s) 337, 658
BseBI CCWGG 1 cut(s) 884
BseDI CCNNGG 1 cut(s) 227
BseGI GGATG 3 cut(s) 528, 559, 637
BseMI GCAATG 2 cut(s) 337, 658
BseMII CTCAG 2 cut(s) 764, 875
BseNI ACTGG 1 cut(s) 541
BseRI GAGGAG 1 cut(s) 216
BshFI GGCC 1 cut(s) 716
BslFI GGGAC 2 cut(s) 225, 767
BsmAI GTCTC 3 cut(s) 38, 143, 288
BsmBI CGTCTC 1 cut(s) 38
BsmFI GGGAC 2 cut(s) 225, 767
BsnI GGCC 1 cut(s) 716
Bsp1407I TGTACA 2 cut(s) 934, 969
Bsp143I GATC 1 cut(s) 294
BspACI CCGC 1 cut(s) 897
BspANI GGCC 1 cut(s) 716
BspCNI CTCAG 2 cut(s) 765, 874
BspLI GGNNCC 2 cut(s) 222, 241
BspTI CTTAAG 2 cut(s) 350, 413
BsrDI GCAATG 2 cut(s) 337, 658
BsrGI TGTACA 2 cut(s) 934, 969
BsrI ACTGG 1 cut(s) 541
BssECI CCNNGG 1 cut(s) 227
BssMI GATC 1 cut(s) 294
BssSI CACGAG 1 cut(s) 60
Bst2BI CACGAG 1 cut(s) 60
Bst2UI CCWGG 1 cut(s) 884
Bst4CI ACNGT 5 cut(s) 53, 103, 474, 758, 769
Bst6I CTCTTC 1 cut(s) 414
BstAFI CTTAAG 2 cut(s) 350, 413
BstAPI GCANNNNNTGC 1 cut(s) 660
BstAUI TGTACA 2 cut(s) 934, 969
BstBAI YACGTR 1 cut(s) 201
BstC8I GCNNGC 2 cut(s) 403, 665
BstDEI CTNAG 3 cut(s) 564, 773, 861
BstEII GGTNACC 1 cut(s) 426
BstF5I GGATG 3 cut(s) 528, 559, 637
BstKTI GATC 1 cut(s) 297
BstMAI GTCTC 3 cut(s) 38, 143, 288
BstMBI GATC 1 cut(s) 294
BstMWI GCNNNNNNNGC 3 cut(s) 159, 632, 660
BstNI CCWGG 1 cut(s) 884
BstNSI RCATGY 1 cut(s) 405
BstPI GGTNACC 1 cut(s) 426
BstSCI CCNGG 1 cut(s) 882
BsuRI GGCC 1 cut(s) 716
BtsCI GGATG 3 cut(s) 528, 559, 637
BtsI GCAGTG 1 cut(s) 297
BtsIMutI CAGTG 2 cut(s) 297, 763
Cac8I GCNNGC 2 cut(s) 403, 665
Cfr13I GGNCC 2 cut(s) 239, 538
CseI GACGC 1 cut(s) 407
Csp6I GTAC 6 cut(s) 127, 369, 770, 794, 935, 970
CviAII CATG 5 cut(s) 123, 402, 535, 955, 973
CviJI RGCY 7 cut(s) 162, 341, 355, 504, 716, 790, 822
CviKI_1 RGCY 7 cut(s) 162, 341, 355, 504, 716, 790, 822
CviQI GTAC 6 cut(s) 127, 369, 770, 794, 935, 970
DdeI CTNAG 3 cut(s) 564, 773, 861
DpnI GATC 1 cut(s) 296
DpnII GATC 1 cut(s) 294
Eam1104I CTCTTC 1 cut(s) 414
EarI CTCTTC 1 cut(s) 414
Eco147I AGGCCT 1 cut(s) 716
Eco47I GGWCC 2 cut(s) 239, 538
Eco91I GGTNACC 1 cut(s) 426
EcoO65I GGTNACC 1 cut(s) 426
EcoRI GAATTC 1 cut(s) 362
EcoRII CCWGG 1 cut(s) 882
Esp3I CGTCTC 1 cut(s) 38
FaeI CATG 5 cut(s) 126, 405, 538, 958, 976
FaqI GGGAC 2 cut(s) 225, 767
FatI CATG 5 cut(s) 122, 401, 534, 954, 972
FblI GTMKAC 1 cut(s) 559
FokI GGATG 3 cut(s) 535, 566, 644
FspBI CTAG 1 cut(s) 696
HaeIII GGCC 1 cut(s) 716
HgaI GACGC 1 cut(s) 407
Hin1II CATG 5 cut(s) 126, 405, 538, 958, 976
HinfI GANTC 4 cut(s) 15, 21, 58, 86
HphI GGTGA 3 cut(s) 454, 716, 911
Hpy166II GTNNAC 3 cut(s) 198, 560, 578
Hpy188I TCNGA 4 cut(s) 20, 231, 397, 864
Hpy188III TCNNGA 7 cut(s) 72, 97, 173, 292, 451, 646, 752
Hpy8I GTNNAC 3 cut(s) 198, 560, 578
HpyAV CCTTC 4 cut(s) 28, 371, 812, 821
HpyCH4III ACNGT 5 cut(s) 53, 103, 474, 758, 769
HpyCH4IV ACGT 2 cut(s) 129, 200
HpyCH4V TGCA 5 cut(s) 117, 302, 635, 663, 783
HpyF10VI GCNNNNNNNGC 3 cut(s) 159, 632, 660
HpyF3I CTNAG 3 cut(s) 564, 773, 861
HpySE526I ACGT 2 cut(s) 129, 200
Hsp92II CATG 5 cut(s) 126, 405, 538, 958, 976
Kzo9I GATC 1 cut(s) 294
LmnI GCTCC 1 cut(s) 99
LpnPI CCDG 9 cut(s) 85, 110, 186, 372, 444, 554, 659, 869, 896
LweI GCATC 1 cut(s) 622
MaeI CTAG 1 cut(s) 696
MaeII ACGT 2 cut(s) 129, 200
MaeIII GTNAC 3 cut(s) 130, 426, 763
MalI GATC 1 cut(s) 296
MboI GATC 1 cut(s) 294
MboII GAAGA 4 cut(s) 17, 401, 604, 851
MluCI AATT 5 cut(s) 362, 491, 636, 744, 851
MlyI GAGTC 2 cut(s) 52, 95
MmeI TCCRAC 1 cut(s) 58
MroXI GAANNNNTTC 1 cut(s) 608
MseI TTAA 5 cut(s) 351, 414, 680, 747, 965
MspCI CTTAAG 2 cut(s) 350, 413
MspR9I CCNGG 1 cut(s) 884
MvaI CCWGG 1 cut(s) 884
MwoI GCNNNNNNNGC 3 cut(s) 159, 632, 660
NdeII GATC 1 cut(s) 294
NlaIII CATG 5 cut(s) 126, 405, 538, 958, 976
NlaIV GGNNCC 2 cut(s) 222, 241
NspI RCATGY 1 cut(s) 405
PaeI GCATGC 1 cut(s) 405
PceI AGGCCT 1 cut(s) 716
PdmI GAANNNNTTC 1 cut(s) 608
PfeI GAWTC 2 cut(s) 15, 21
PleI GAGTC 2 cut(s) 52, 94
PpsI GAGTC 2 cut(s) 52, 94
Ppu21I YACGTR 1 cut(s) 201
PshBI ATTAAT 1 cut(s) 747
Psp6I CCWGG 1 cut(s) 882
PspEI GGTNACC 1 cut(s) 426
PspGI CCWGG 1 cut(s) 882
PspN4I GGNNCC 2 cut(s) 222, 241
PspPI GGNCC 2 cut(s) 239, 538
RsaI GTAC 6 cut(s) 128, 370, 771, 795, 936, 971
RsaNI GTAC 6 cut(s) 127, 369, 770, 794, 935, 970
SaqAI TTAA 5 cut(s) 351, 414, 680, 747, 965
Sau3AI GATC 1 cut(s) 294
Sau96I GGNCC 2 cut(s) 239, 538
SchI GAGTC 2 cut(s) 52, 95
ScrFI CCNGG 1 cut(s) 884
SetI ASST 9 cut(s) 132, 203, 226, 343, 357, 506, 565, 792, 947
SfaNI GCATC 1 cut(s) 622
SinI GGWCC 2 cut(s) 239, 538
SmlI CTYRAG 3 cut(s) 350, 413, 718
SmoI CTYRAG 3 cut(s) 350, 413, 718
SpeI ACTAGT 1 cut(s) 695
SphI GCATGC 1 cut(s) 405
Sse9I AATT 5 cut(s) 362, 491, 636, 744, 851
SseBI AGGCCT 1 cut(s) 716
SsiI CCGC 1 cut(s) 897
SspMI CTAG 1 cut(s) 696
StuI AGGCCT 1 cut(s) 716
StyD4I CCNGG 1 cut(s) 882
TaaI ACNGT 5 cut(s) 53, 103, 474, 758, 769
TaiI ACGT 2 cut(s) 132, 203
TaqI TCGA 2 cut(s) 293, 742
TasI AATT 5 cut(s) 362, 491, 636, 744, 851
TatI WGTACW 4 cut(s) 769, 793, 934, 969
TfiI GAWTC 2 cut(s) 15, 21
Tru1I TTAA 5 cut(s) 351, 414, 680, 747, 965
Tru9I TTAA 5 cut(s) 351, 414, 680, 747, 965
TscAI CASTG 2 cut(s) 304, 763
TspDTI ATGAA 4 cut(s) 561, 617, 852, 884
TspGWI ACGGA 2 cut(s) 62, 689
TspRI CASTG 2 cut(s) 304, 763
Vha464I CTTAAG 2 cut(s) 350, 413
VpaK11BI GGWCC 2 cut(s) 239, 538
VspI ATTAAT 1 cut(s) 747
XapI RAATTY 3 cut(s) 362, 491, 851
XceI RCATGY 1 cut(s) 405
XcmI CCANNNNNNNNNTGG 1 cut(s) 79
XmiI GTMKAC 1 cut(s) 559
XmnI GAANNNNTTC 1 cut(s) 608
XspI CTAG 1 cut(s) 696
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.