FvH4_4g02430

Sieve element occlusion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000511
Physical Location & Seq
Forward (+)
2227945 .. 2231192
3248 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g02430.t2

Sequence Viewer

Length: 2016 bp
ATGCTTGGCCTAGTAAACAAGGTGGCCTCAGTAATCCACCACCCTCATAGCCCGTTTGCCAAGTCTGACAAGAAGATCTTGGATGATATTTACACTACTCATGTCCATGCTGATGATTCATTCGACGACGATTCTCTTTTCATCATTGTTGAGAGTATCCTCAAGCGTGCAACCCAAACTGTCGATAAAATTGTGCAGGGTACCCAAGTGCATGTGGAAGACATAGAGGAGCAAACTCCCAAAGCCAACTTCAGTGTACCATTGTGCACGCTTAAGGGAATCTCCTGCGAGATGCAATGCAAGCCTCCTGGTGAAGAAATCGCCCATGAGACAACACTGGCAATACTACACAAGCTGTCAAAGTATTCATGGGAAGCAAAGGCATCACTGACCCTAGCAGCTTTTGCTATGGAGTTTGGTGAATTCTGGCTCCTTGCAGAGCTTCGTGAATCAAACCACCTTGCCAGGTCAATTGCAATTCTCAAACGACTCCCTGTTCTCCTCAAGCCTTCTCAGCTGCACAAGCGCAGGCAAGCAATACTTGAGCTTAACAATCTGATCAAGGCCACACTGGAAGTCATTACATGCATCGACCAGTTCAACAAGATTTCCATTTATGACCTGAAAGATGTCCCCGAGTTGTCGGGAACATTGGATCATGTTCCGGTGGACGTATATTGGGCTATCATGACTGTTGTGGCTTGTGCCACTAAGATCACACTACTCACTAGTGATGAGGACAAAGAATTTGACCTATCTCCCTATTCTCAAAAGATCCACTTTGTCCTTAACAAGCTCAAGACACAGCTAACAATTTGCAGAGCACGAATTGAGTTCATAGAAAATTATAAAAAGCTCAAGAAACTGTTCCGAACACCTACTGAAATTATGGAAATCTTTAAGGGTTTGTTATTTACAAAGGATAATGTCCAGCCACTCGTTGATTGTTCCACTAAACAAACGGTGAGCATCGAAATCCTAAGGAGGAAGAATGTGCTATTCTTCATTTCCAGCCTCGACATTACGGATGACGACATTTCGATTCTGAAACCGGTCCACGAGTTCACAAAGAAAGACAATCAGTATAAGATTGTGTGGATACCGATTGTGGAGCAATGGACCGATGAGCTGAGAAAGAAATTTGAAATCCTGAAGAACAAGATGCCGTGGTATACGGTGCAGTACTCTGGACCTATTGCAGGCATCAAGTTCATCAAGGAGGAGTGGAACTTCAAGGGTAAGCCATTACTGGTAGTGATGAACCCACAAGGAAAGGTCGAACACACTAACGCTCTTCACATGATTCGGGTGTGGGGAGTCAAGGCCTTCCCTTTCACAGAGACGATAGAGAAAGAGCTGTCATCAGATTCTCATGGAGGAATTCACTCTATAGTTGTTGACGGAATTCACCCTTCTGTGCCATCTTACATCAGAGATAATAAGTACATCTTCTTCTATGGCGGTAAGGACAACGAATGGATTCAGCAATTCACAAAGAAAGCAACTGCCCTTGCAAACGATCCTATCTTGAAGGAAGCAAAGATTTACATAGAGTTGGTTTGCGTTGGAAAAGGCAGCGAAGGAGAAGATGACCATGGCATTCTAGGCCGCTTCTGGACCGGTATAGAAAGTTTGTTCCTCACCAAAGTCCACAAGCACGTTGATCCGATCGGTCAAGAAATTCAGAAGCTTGTCTCTTACAAGAACGAAAGTGGATGGGTGTTGCTTACCAAAGGATCAACTTTGTTGGTCACAGGTCATGGTATCTCCGCATTGAAGGTGGTAGAAGACTTTGAGAAATGGAGGGAGCATGTCAAGGAGAAAGGGTTTGAGTTTTGTTTCAAGGCCTATTATGGGAAGGTTATTCAGGCTGGTCGACCTTGTTGCCGTCTTGACATTCCGGGGTCTACCGGAAAGGTTCCGGAGTCGATGAAGTGCCCTGATTGTCACCGTAGTATGGAGACTTTTATCAGTTACAAGTGCTGCCACATTGATGGTCCAACTGCACATCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SEO_C SEO_N

Protein Analysis

672

Amino Acids

76.49

Weight (kDa)

7.34

Isoelectric Point (pI)

34.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SEO_N PF14576 1 - 196 7.6e-63 Sieve element occlusion N-terminus
SEO_C PF14577 367 - 566 6.7e-44 Sieve element occlusion C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000511)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g02370 FvH4_4g02370 FvH4_4g02370 FvH4_4g02430 FvH4_4g02430 FvH4_4g02460 FvH4_4g02460 FvH4_4g02460 FvH4_4g02480 FvH4_4g02480 FvH4_4g02480 FvH4_4g02480 FvH4_6g05920
malus_domestica MD00G1111000.v1.1 MD08G1225800.v1.1 MD13G1205700.v1.1 MD13G1205800.v1.1 MD13G1205900.v1.1 MD13G1206000.v1.1 MD16G1207500.v1.1 MD16G1207600.v1.1
prunus_persica Prupe.1G028400_v2.0.a1 Prupe.1G028500_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028900_v2.0.a1 Prupe.1G029000_v2.0.a1 Prupe.1G029100_v2.0.a1
pyrus_communis pycom08g19680 pycom08g19730 pycom16g17460 pycom16g17500
rosa_chinensis RchiOBHm_Chr4g0389751 RchiOBHm_Chr4g0389771 RchiOBHm_Chr4g0389791 RchiOBHm_Chr4g0389861 RchiOBHm_Chr4g0389881 RchiOBHm_Chr4g0389901 RchiOBHm_Chr4g0412411
rosa_laevigata RLG00000008288 RLG00000009901 RLG00000009945 RLG00000009954 RLG00000009956 RLG00000009957 RLG00000009958 RLG00000019246
rosa_multiflora Rmu_co8275125.1_g000001 Rmu_sc0002083.1_g000003 Rmu_sc0002083.1_g000025 Rmu_sc0002604.1_g000016 Rmu_sc0004336.1_g000031 Rmu_sc0010423.1_g000003
rosa_roxburghii Rroxscaffold_5G00335790 Rroxscaffold_5G00335850 Rroxscaffold_5G00335970 Rroxscaffold_5G00335990
rosa_rugosa Rorug03G0325100 Rorug03G0325300 Rorug03G0325500.1 Rorug03G0326800 Rorug03G0327000 Rorug04G0104400
rosa_samantha Rh4AG029900 Rh4AG030000 Rh4AG030100 Rh4AG030200 Rh4AG030600 Rh4AG172900 Rh4BG023200 Rh4BG023900 Rh4BG024000 Rh4BG024100 Rh4BG172200 Rh4DG023300 Rh4DG023800 Rh4DG023900 Rh4DG024000 Rh4DG151600
rosa_wichuraiana Rw1G002330 Rw1G002340 Rw4G002260 Rw4G002270 Rw4G002280 Rw4G002300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 849
Acc65I GGTACC 1 cut(s) 200
AccB1I GGYRCC 1 cut(s) 200
AccI GTMKAC 3 cut(s) 1172, 1876, 1907
AccIII TCCGGA 1 cut(s) 1921
AciI CCGC 3 cut(s) 1461, 1609, 1770
AclWI GGATC 5 cut(s) 663, 769, 1514, 1658, 1744
AcsI RAATTY 6 cut(s) 422, 746, 1139, 1380, 1404, 1680
AcuI CTGAAG 2 cut(s) 235, 1172
AfaI GTAC 4 cut(s) 202, 258, 1184, 1445
AfiI CCNNNNNNNGG 3 cut(s) 1199, 1854, 1957
AflII CTTAAG 1 cut(s) 272
AgeI ACCGGT 2 cut(s) 1051, 1619
AgsI TTSAA 6 cut(s) 601, 1145, 1234, 1531, 1777, 1843
AhlI ACTAGT 1 cut(s) 728
AjnI CCWGG 2 cut(s) 307, 464
AjuI GAANNNNNNNTTGG 2 cut(s) 233, 265
Alw21I GWGCWC 2 cut(s) 269, 826
Alw26I GTCTC 4 cut(s) 323, 1334, 1699, 1955
Alw44I GTGCAC 1 cut(s) 265
AlwI GGATC 5 cut(s) 663, 769, 1514, 1658, 1744
Ama87I CYCGRG 1 cut(s) 635
Aor13HI TCCGGA 1 cut(s) 1921
AoxI GGCC 6 cut(s) 7, 24, 564, 1323, 1606, 1845
ApaLI GTGCAC 1 cut(s) 265
ApeKI GCWGC 4 cut(s) 398, 517, 1575, 1983
ApoI RAATTY 6 cut(s) 422, 746, 1139, 1380, 1404, 1680
ArsI GACNNNNNNTTYG 2 cut(s) 731, 763
AsiGI ACCGGT 2 cut(s) 1051, 1619
Asp700I GAANNNNTTC 2 cut(s) 866, 1479
Asp718I GGTACC 1 cut(s) 200
AspLEI GCGC 1 cut(s) 528
AspS9I GGNCC 5 cut(s) 1054, 1119, 1190, 1617, 1997
AsuC2I CCSGG 1 cut(s) 1902
AsuHPI GGTGA 6 cut(s) 323, 431, 976, 1400, 1633, 1940
AvaI CYCGRG 1 cut(s) 635
AvaII GGWCC 5 cut(s) 1054, 1119, 1190, 1617, 1997
AxyI CCTNAGG 1 cut(s) 980
BaeGI GKGCMC 2 cut(s) 269, 1940
BaeI ACNNNNGTAYC 2 cut(s) 192, 225
BanI GGYRCC 1 cut(s) 200
BarI GAAGNNNNNNTAC 2 cut(s) 1436, 1468
BauI CACGAG 1 cut(s) 1058
BbsI GAAGAC 2 cut(s) 225, 1794
Bbv12I GWGCWC 2 cut(s) 269, 826
BbvI GCAGC 4 cut(s) 410, 504, 1587, 1970
BccI CCATC 3 cut(s) 1429, 1710, 1988
BceAI ACGGC 2 cut(s) 1150, 1872
BcgI CGANNNNNNTGC 2 cut(s) 1866, 1900
BciT130I CCWGG 2 cut(s) 309, 466
BciVI GTATCC 2 cut(s) 167, 1092
BclI TGATCA 1 cut(s) 558
BcnI CCSGG 1 cut(s) 1902
BcoDI GTCTC 4 cut(s) 323, 1334, 1699, 1955
BcuI ACTAGT 1 cut(s) 728
BfaI CTAG 4 cut(s) 11, 395, 729, 1604
BfmI CTRYAG 1 cut(s) 1389
BfrI CTTAAG 1 cut(s) 272
BfuI GTATCC 2 cut(s) 167, 1092
BglII AGATCT 1 cut(s) 75
BisI GCNGC 5 cut(s) 399, 518, 1576, 1609, 1984
BlsI GCNGC 5 cut(s) 400, 519, 1577, 1610, 1985
BmcAI AGTACT 1 cut(s) 1184
Bme1390I CCNGG 3 cut(s) 309, 466, 1902
Bme18I GGWCC 5 cut(s) 1054, 1119, 1190, 1617, 1997
BmeT110I CYCGRG 1 cut(s) 635
BmgT120I GGNCC 5 cut(s) 1054, 1119, 1190, 1617, 1997
BmiI GGNNCC 3 cut(s) 202, 431, 1920
BmrFI CCNGG 3 cut(s) 309, 466, 1902
BmsI GCATC 6 cut(s) 282, 392, 597, 978, 1152, 1212
BpiI GAAGAC 2 cut(s) 225, 1794
BpuEI CTTGAG 5 cut(s) 146, 488, 563, 782, 842
BpuMI CCSGG 1 cut(s) 1902
BsaJI CCNNGG 3 cut(s) 1166, 1594, 1901
BsaWI WCCGGW 5 cut(s) 664, 1051, 1619, 1910, 1921
BsaXI ACNNNNNCTCC 2 cut(s) 1811, 1841
Bsc4I CCNNNNNNNGG 3 cut(s) 1199, 1854, 1957
Bse118I RCCGGY 2 cut(s) 1051, 1619
Bse1I ACTGG 4 cut(s) 342, 576, 595, 1254
Bse21I CCTNAGG 1 cut(s) 980
Bse3DI GCAATG 2 cut(s) 302, 1121
BseAI TCCGGA 1 cut(s) 1921
BseBI CCWGG 2 cut(s) 309, 466
BseDI CCNNGG 3 cut(s) 1166, 1594, 1901
BseGI GGATG 3 cut(s) 88, 1033, 1721
BseLI CCNNNNNNNGG 3 cut(s) 1199, 1854, 1957
BseMI GCAATG 2 cut(s) 302, 1121
BseMII CTCAG 3 cut(s) 42, 527, 1121
BseNI ACTGG 4 cut(s) 342, 576, 595, 1254
BseRI GAGGAG 3 cut(s) 242, 491, 1235
BseSI GKGCMC 2 cut(s) 269, 1940
BseXI GCAGC 4 cut(s) 410, 504, 1587, 1970
BsgI GTGCAG 4 cut(s) 215, 503, 1199, 1989
Bsh1285I CGRYCG 1 cut(s) 1671
BshFI GGCC 6 cut(s) 9, 26, 566, 1325, 1608, 1847
BshNI GGYRCC 1 cut(s) 200
BshTI ACCGGT 2 cut(s) 1051, 1619
BsiEI CGRYCG 1 cut(s) 1671
BsiHKAI GWGCWC 2 cut(s) 269, 826
BsiHKCI CYCGRG 1 cut(s) 635
BsiSI CCGG 6 cut(s) 665, 1052, 1620, 1901, 1911, 1922
BslFI GGGAC 1 cut(s) 617
BslI CCNNNNNNNGG 3 cut(s) 1199, 1854, 1957
BsmAI GTCTC 4 cut(s) 323, 1334, 1699, 1955
BsmBI CGTCTC 1 cut(s) 1334
BsmFI GGGAC 1 cut(s) 617
BsmI GAATGC 1 cut(s) 1599
BsnI GGCC 6 cut(s) 9, 26, 566, 1325, 1608, 1847
BsoBI CYCGRG 1 cut(s) 635
Bsp1286I GDGCHC 3 cut(s) 269, 826, 1940
Bsp13I TCCGGA 1 cut(s) 1921
Bsp143I GATC 9 cut(s) 75, 558, 655, 714, 774, 1519, 1663, 1668, 1736
Bsp19I CCATGG 1 cut(s) 1594
BspACI CCGC 3 cut(s) 1461, 1609, 1770
BspANI GGCC 6 cut(s) 9, 26, 566, 1325, 1608, 1847
BspCNI CTCAG 3 cut(s) 41, 526, 1122
BspEI TCCGGA 1 cut(s) 1921
BspHI TCATGA 1 cut(s) 687
BspLI GGNNCC 3 cut(s) 202, 431, 1920
BspPI GGATC 5 cut(s) 663, 769, 1514, 1658, 1744
BspQI GCTCTTC 1 cut(s) 1299
BspT107I GGYRCC 1 cut(s) 200
BspTI CTTAAG 1 cut(s) 272
BsrDI GCAATG 2 cut(s) 302, 1121
BsrFI RCCGGY 2 cut(s) 1051, 1619
BsrI ACTGG 4 cut(s) 342, 576, 595, 1254
BssAI RCCGGY 2 cut(s) 1051, 1619
BssECI CCNNGG 3 cut(s) 1166, 1594, 1901
BssMI GATC 9 cut(s) 75, 558, 655, 714, 774, 1519, 1663, 1668, 1736
BssNAI GTATAC 1 cut(s) 1173
BssSI CACGAG 1 cut(s) 1058
BssT1I CCWWGG 1 cut(s) 1594
Bst1107I GTATAC 1 cut(s) 1173
Bst2BI CACGAG 1 cut(s) 1058
Bst2UI CCWGG 2 cut(s) 309, 466
Bst4CI ACNGT 6 cut(s) 181, 694, 867, 964, 1177, 1952
Bst6I CTCTTC 1 cut(s) 1299
BstAFI CTTAAG 1 cut(s) 272
BstAPI GCANNNNNTGC 1 cut(s) 404
BstC8I GCNNGC 6 cut(s) 168, 269, 302, 530, 534, 1201
BstDEI CTNAG 5 cut(s) 28, 513, 711, 980, 1130
BstDSI CCRYGG 2 cut(s) 1166, 1594
BstENI CCTNNNNNAGG 1 cut(s) 1197
BstF5I GGATG 3 cut(s) 88, 1033, 1721
BstHHI GCGC 1 cut(s) 528
BstKTI GATC 9 cut(s) 78, 561, 658, 717, 777, 1522, 1666, 1671, 1739
BstMAI GTCTC 4 cut(s) 323, 1334, 1699, 1955
BstMBI GATC 9 cut(s) 75, 558, 655, 714, 774, 1519, 1663, 1668, 1736
BstMCI CGRYCG 1 cut(s) 1671
BstMWI GCNNNNNNNGC 5 cut(s) 301, 404, 514, 523, 1605
BstNI CCWGG 2 cut(s) 309, 466
BstNSI RCATGY 3 cut(s) 215, 588, 1814
BstSCI CCNGG 3 cut(s) 307, 464, 1900
BstSFI CTRYAG 1 cut(s) 1389
BstSLI GKGCMC 2 cut(s) 269, 1940
BstV1I GCAGC 4 cut(s) 410, 504, 1587, 1970
BstV2I GAAGAC 2 cut(s) 225, 1794
BstX2I RGATCY 2 cut(s) 75, 774
BstXI CCANNNNNNTGG 1 cut(s) 1994
BstYI RGATCY 2 cut(s) 75, 774
BstZ17I GTATAC 1 cut(s) 1173
Bsu36I CCTNAGG 1 cut(s) 980
BsuI GTATCC 2 cut(s) 167, 1092
BsuRI GGCC 6 cut(s) 9, 26, 566, 1325, 1608, 1847
BtgI CCRYGG 2 cut(s) 1166, 1594
BtsCI GGATG 3 cut(s) 88, 1033, 1721
BtsIMutI CAGTG 4 cut(s) 259, 335, 386, 569
Cac8I GCNNGC 6 cut(s) 168, 269, 302, 530, 534, 1201
CciI TCATGA 1 cut(s) 687
CfoI GCGC 1 cut(s) 528
Cfr10I RCCGGY 2 cut(s) 1051, 1619
Cfr13I GGNCC 5 cut(s) 1054, 1119, 1190, 1617, 1997
Csp6I GTAC 4 cut(s) 201, 257, 1183, 1444
CspAI ACCGGT 2 cut(s) 1051, 1619
CviQI GTAC 4 cut(s) 201, 257, 1183, 1444
DdeI CTNAG 5 cut(s) 28, 513, 711, 980, 1130
DpnI GATC 9 cut(s) 77, 560, 657, 716, 776, 1521, 1665, 1670, 1738
DpnII GATC 9 cut(s) 75, 558, 655, 714, 774, 1519, 1663, 1668, 1736
Eam1104I CTCTTC 1 cut(s) 1299
EarI CTCTTC 1 cut(s) 1299
Eco130I CCWWGG 1 cut(s) 1594
Eco147I AGGCCT 2 cut(s) 1325, 1847
Eco47I GGWCC 5 cut(s) 1054, 1119, 1190, 1617, 1997
Eco57I CTGAAG 2 cut(s) 235, 1172
Eco81I CCTNAGG 1 cut(s) 980
Eco88I CYCGRG 1 cut(s) 635
EcoNI CCTNNNNNAGG 1 cut(s) 1197
EcoRI GAATTC 3 cut(s) 422, 1380, 1404
EcoRII CCWGG 2 cut(s) 307, 464
EcoT14I CCWWGG 1 cut(s) 1594
EcoT22I ATGCAT 1 cut(s) 590
ErhI CCWWGG 1 cut(s) 1594
Esp3I CGTCTC 1 cut(s) 1334
FalI AAGNNNNNCTT 8 cut(s) 62, 94, 525, 557, 764, 796, 1433, 1465
FaqI GGGAC 1 cut(s) 617
FbaI TGATCA 1 cut(s) 558
FblI GTMKAC 3 cut(s) 1172, 1876, 1907
Fnu4HI GCNGC 5 cut(s) 399, 518, 1576, 1609, 1984
FokI GGATG 3 cut(s) 95, 1040, 1728
Fsp4HI GCNGC 5 cut(s) 399, 518, 1576, 1609, 1984
FspBI CTAG 4 cut(s) 11, 395, 729, 1604
GlaI GCGC 1 cut(s) 527
GluI GCNGC 5 cut(s) 399, 518, 1576, 1609, 1984
HaeIII GGCC 6 cut(s) 9, 26, 566, 1325, 1608, 1847
HapII CCGG 6 cut(s) 665, 1052, 1620, 1901, 1911, 1922
HhaI GCGC 1 cut(s) 528
Hin6I GCGC 1 cut(s) 526
HinP1I GCGC 1 cut(s) 526
HincII GTYRAC 2 cut(s) 1399, 1877
HindII GTYRAC 2 cut(s) 1399, 1877
HindIII AAGCTT 1 cut(s) 1688
HpaII CCGG 6 cut(s) 665, 1052, 1620, 1901, 1911, 1922
HphI GGTGA 6 cut(s) 323, 431, 976, 1400, 1633, 1940
Hpy188I TCNGA 8 cut(s) 67, 558, 872, 1047, 1366, 1433, 1668, 1686
Hpy99I CGWCG 2 cut(s) 128, 131
HpyAV CCTTC 7 cut(s) 519, 1336, 1422, 1525, 1574, 1771, 1852
HpyCH4III ACNGT 6 cut(s) 181, 694, 867, 964, 1177, 1952
HpyCH4IV ACGT 2 cut(s) 672, 1659
HpyF10VI GCNNNNNNNGC 5 cut(s) 301, 404, 514, 523, 1605
HpyF3I CTNAG 5 cut(s) 28, 513, 711, 980, 1130
HpySE526I ACGT 2 cut(s) 672, 1659
HspAI GCGC 1 cut(s) 526
Kpn2I TCCGGA 1 cut(s) 1921
KpnI GGTACC 1 cut(s) 204
Ksp22I TGATCA 1 cut(s) 558
Kzo9I GATC 9 cut(s) 75, 558, 655, 714, 774, 1519, 1663, 1668, 1736
LguI GCTCTTC 1 cut(s) 1299
LmnI GCTCC 4 cut(s) 229, 435, 1111, 1807
Lsp1109I GCAGC 4 cut(s) 410, 504, 1587, 1970
LweI GCATC 6 cut(s) 282, 392, 597, 978, 1152, 1212
MaeI CTAG 4 cut(s) 11, 395, 729, 1604
MaeII ACGT 2 cut(s) 672, 1659
MaeIII GTNAC 3 cut(s) 1750, 1946, 1973
MalI GATC 9 cut(s) 77, 560, 657, 716, 776, 1521, 1665, 1670, 1738
MboI GATC 9 cut(s) 75, 558, 655, 714, 774, 1519, 1663, 1668, 1736
MfeI CAATTG 1 cut(s) 471
MflI RGATCY 2 cut(s) 75, 774
MhlI GDGCHC 3 cut(s) 269, 826, 1940
MlyI GAGTC 3 cut(s) 483, 1326, 1934
MmeI TCCRAC 1 cut(s) 1546
Mph1103I ATGCAT 1 cut(s) 590
MroI TCCGGA 1 cut(s) 1921
MroXI GAANNNNTTC 2 cut(s) 866, 1479
MseI TTAA 4 cut(s) 273, 549, 789, 900
MslI CAYNNNNRTG 3 cut(s) 105, 111, 1992
MspA1I CMGCKG 1 cut(s) 517
MspCI CTTAAG 1 cut(s) 272
MspI CCGG 6 cut(s) 665, 1052, 1620, 1901, 1911, 1922
MspR9I CCNGG 3 cut(s) 309, 466, 1902
MunI CAATTG 1 cut(s) 471
Mva1269I GAATGC 1 cut(s) 1599
MvaI CCWGG 2 cut(s) 309, 466
MwoI GCNNNNNNNGC 5 cut(s) 301, 404, 514, 523, 1605
NciI CCSGG 1 cut(s) 1902
NcoI CCATGG 1 cut(s) 1594
NdeII GATC 9 cut(s) 75, 558, 655, 714, 774, 1519, 1663, 1668, 1736
NlaIV GGNNCC 3 cut(s) 202, 431, 1920
NmuCI GTSAC 2 cut(s) 1750, 1946
NsiI ATGCAT 1 cut(s) 590
NspI RCATGY 3 cut(s) 215, 588, 1814
PagI TCATGA 1 cut(s) 687
PceI AGGCCT 2 cut(s) 1325, 1847
PciSI GCTCTTC 1 cut(s) 1299
PctI GAATGC 1 cut(s) 1599
PdmI GAANNNNTTC 2 cut(s) 866, 1479
PfeI GAWTC 8 cut(s) 116, 131, 279, 449, 1042, 1303, 1367, 1480
PinAI ACCGGT 2 cut(s) 1051, 1619
PkrI GCNGC 5 cut(s) 400, 519, 1577, 1610, 1985
Ple19I CGATCG 1 cut(s) 1671
PleI GAGTC 3 cut(s) 483, 1325, 1933
PpsI GAGTC 3 cut(s) 483, 1325, 1933
PsiI TTATAA 1 cut(s) 849
Psp6I CCWGG 2 cut(s) 307, 464
PspGI CCWGG 2 cut(s) 307, 464
PspN4I GGNNCC 3 cut(s) 202, 431, 1920
PspPI GGNCC 5 cut(s) 1054, 1119, 1190, 1617, 1997
PsuI RGATCY 2 cut(s) 75, 774
PvuI CGATCG 1 cut(s) 1671
PvuII CAGCTG 1 cut(s) 517
RsaI GTAC 4 cut(s) 202, 258, 1184, 1445
RsaNI GTAC 4 cut(s) 201, 257, 1183, 1444
RseI CAYNNNNRTG 3 cut(s) 105, 111, 1992
SalI GTCGAC 1 cut(s) 1875
SapI GCTCTTC 1 cut(s) 1299
SaqAI TTAA 4 cut(s) 273, 549, 789, 900
SatI GCNGC 5 cut(s) 399, 518, 1576, 1609, 1984
Sau3AI GATC 9 cut(s) 75, 558, 655, 714, 774, 1519, 1663, 1668, 1736
Sau96I GGNCC 5 cut(s) 1054, 1119, 1190, 1617, 1997
ScaI AGTACT 1 cut(s) 1184
SchI GAGTC 3 cut(s) 483, 1326, 1934
ScrFI CCNGG 3 cut(s) 309, 466, 1902
SduI GDGCHC 3 cut(s) 269, 826, 1940
SfaNI GCATC 6 cut(s) 282, 392, 597, 978, 1152, 1212
SfcI CTRYAG 1 cut(s) 1389
SinI GGWCC 5 cut(s) 1054, 1119, 1190, 1617, 1997
SmiMI CAYNNNNRTG 3 cut(s) 105, 111, 1992
SmlI CTYRAG 6 cut(s) 161, 272, 503, 542, 797, 857
SmoI CTYRAG 6 cut(s) 161, 272, 503, 542, 797, 857
SpeI ACTAGT 1 cut(s) 728
SseBI AGGCCT 2 cut(s) 1325, 1847
SsiI CCGC 3 cut(s) 1461, 1609, 1770
SspMI CTAG 4 cut(s) 11, 395, 729, 1604
StuI AGGCCT 2 cut(s) 1325, 1847
StyD4I CCNGG 3 cut(s) 307, 464, 1900
StyI CCWWGG 1 cut(s) 1594
TaaI ACNGT 6 cut(s) 181, 694, 867, 964, 1177, 1952
TaiI ACGT 2 cut(s) 675, 1662
TaqI TCGA 9 cut(s) 123, 183, 591, 972, 1017, 1040, 1278, 1876, 1928
TaqII GACCGA 2 cut(s) 1136, 1661
TatI WGTACW 2 cut(s) 1182, 1443
TauI GCSGC 1 cut(s) 1611
TfiI GAWTC 8 cut(s) 116, 131, 279, 449, 1042, 1303, 1367, 1480
Tru1I TTAA 4 cut(s) 273, 549, 789, 900
Tru9I TTAA 4 cut(s) 273, 549, 789, 900
TscAI CASTG 4 cut(s) 259, 342, 393, 576
TseFI GTSAC 2 cut(s) 1750, 1946
TseI GCWGC 4 cut(s) 398, 517, 1575, 1983
Tsp45I GTSAC 2 cut(s) 1750, 1946
TspDTI ATGAA 8 cut(s) 108, 130, 357, 826, 994, 1201, 1274, 1946
TspGWI ACGGA 2 cut(s) 1040, 1416
TspRI CASTG 4 cut(s) 259, 342, 393, 576
Vha464I CTTAAG 1 cut(s) 272
VneI GTGCAC 1 cut(s) 265
VpaK11BI GGWCC 5 cut(s) 1054, 1119, 1190, 1617, 1997
XagI CCTNNNNNAGG 1 cut(s) 1197
XapI RAATTY 6 cut(s) 422, 746, 1139, 1380, 1404, 1680
XceI RCATGY 3 cut(s) 215, 588, 1814
XmiI GTMKAC 3 cut(s) 1172, 1876, 1907
XmnI GAANNNNTTC 2 cut(s) 866, 1479
XspI CTAG 4 cut(s) 11, 395, 729, 1604
ZrmI AGTACT 1 cut(s) 1184
Zsp2I ATGCAT 1 cut(s) 590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.