MD16G1207500.v1.1

Sieve element occlusion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0000511
Physical Location & Seq
Forward (+)
19424308 .. 19429007
4700 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1207500.v1.1.491

Sequence Viewer

Length: 2046 bp
ATGCTAGGCATAGCACAAAATGTGGTGAGCAAGGTGACCTCGGCGGTCGCCACCACTGCTCAGCACATTGAGGGTGAGCTCAGCCTCTTCACTATGTCCGACACCAAAATATTAGAGCTCATTTATGCAACCCACGTCCATGAGGACGACTCCTTTGATGTTGATTCTCTCTTCCTTGTCACTGAAACCATCATCAAGCATTCGACCCAGATCGTTGATAGCATTGTGCAGGGCACCCAAGTACATGTTGAAACCATTGATGAGAAGCCCCCCAAAGCCACCTTCAGTTCTCCATTGTGCACTCTCAAGTCCATTGGCTGTGAGATGTCATGCAAGCCACCAGGTGAAGAAATTGCCCACAAGTCAACCTTGGCAATACTCAACAAGCTCTCAACTTATTCATGGGAAGCCAAGGCAGTGCTGGCCTTTGCAGCATTTGCTTTGGAATATGGAGAGTTCTGGCTCCTTGCCCAAACTCAACAGAGTGACCTACTTGCTAAGTCCGTCGCAATCCTCAAGAGAGTGCCAGTCCTTCTCAAGCCCACGGACCTCCAGAAGCGCAGGCAAGCTATTGTCGAACTCAACGTCCTGATCAAGACCACATTGCAAGTGATTGAGTGCATCTTTGAGCTTGAGAAGCTTTCTGCTTATGATCCAAAGGATGTGCCAGCATTGGCAATTGCAATGGATCACATACCGGTCGATGTCTATTGGTCTATCATAACTATTTTTTCTTGTGCAACTAAGATCACCCTTCTAACCAGTGATGAAGAGAAGCCATATGATTTGTCCCAATTTGCTCAAAAAATCCACTACATCCTCAACAAGCTTAAGATACAATTGTTAATCTGCAAAAAACAGATAGAGGAGGCGGAGACGTACCGGAAGCTGAGGAAACTTTTTCAGACCCCTGCCGAAGTTATGGAGGTTTTCAAGGCCTTGATTTTCACTAAGGATACCGTGCAGCCAATTATTGATGGTTCGACTAACAAAACGGTTAGCATTGATGTGCTGAGGAGGAAGTATGTGCTTTTGTTCATTTCCACCCTTGACATTTCTGATGATGACATTTCGATTGTCAAACCAGTTTATGAGGGGACAAAGAAAGACGATAAGTATAAGATTGTGTGGATCCCTATTGTGGAGCAATGGACTGATGACCTACGAAAGAAGTTCGAGGTCTTGAGGGCCAAGATGCCATGGTACACTGTGCAGTATTTTGCGCCTGTAGCCGGCGTCAGATTCATCAAGGAGGAGTGGCACTTCAAGGGCAAGCCCGCTGTGGTAGTGATGAACCCGCAAGGCAAGGTGGAAAATACGAATGCTCTCCACTTGATTCGGATTCATGGAATGAAGGCATTTCCTTTTCATAAGGGAATAGAAGATACTCTCACAAATGACAAAGAATGGATCACTCCCATTGTGAATGATATTCACCCAACCATACAGACCTGGATCAAAGAGGAGAAGTACATCTTCTTCTATGGAGGCAAAGACAATGACTGGATCCAGCAGTTCACAAAGAAAGCAACTACCATTGCCAATGACCCCTTCATCAAGGAATTGAAGATCAATATTGAGCTATTTTGTGTTGGCAAGAGCCCGAAAGGCGGAGAAGACCTTGGAATTCTCGGGAGATTTTGGAATGGAATTGAGAGCTTGTTCTTCACCAAAGTCAACAAGCAAACTGACACTGTGACCAAAGAAGTCCAGAAGCTGCTTTCCTACAAAAATGAGGGTGGATGGGCTGTGCTTACTAAGGGGTCCACTGTGGTGGTGAGTGGCCATGGCTTCACAATCTTGAAGGTCCTTGACGACTTCGACACATGGAAGAACTTCATAAAGGAGAAGGGCTTTGAATTCTCGTTCAAAGCATACTACGAGAAGGTGATTCAGACTATGAGGCACTGCTGCCGCCTTGACATCCCGAGCGTTGCTGGAAAGGTCCCGGAGACCATGAAATGCCCAGAGTGTCCTCGCACCATGGAGACATTCGTGAGCTACAAGTGCTGCCACACTGATTCTCCCATCAACGCGCATCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SEO_C SEO_N

Protein Analysis

682

Amino Acids

77.54

Weight (kDa)

7.01

Isoelectric Point (pI)

34.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SEO_N PF14576 33 - 304 4.2e-88 Sieve element occlusion N-terminus
SEO_C PF14577 464 - 536 3e-14 Sieve element occlusion C-terminus
SEO_C PF14577 546 - 672 4.1e-35 Sieve element occlusion C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000511)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g02370 FvH4_4g02370 FvH4_4g02370 FvH4_4g02430 FvH4_4g02430 FvH4_4g02460 FvH4_4g02460 FvH4_4g02460 FvH4_4g02480 FvH4_4g02480 FvH4_4g02480 FvH4_4g02480 FvH4_6g05920
malus_domestica MD00G1111000.v1.1 MD08G1225800.v1.1 MD13G1205700.v1.1 MD13G1205800.v1.1 MD13G1205900.v1.1 MD13G1206000.v1.1 MD16G1207500.v1.1 MD16G1207600.v1.1
prunus_persica Prupe.1G028400_v2.0.a1 Prupe.1G028500_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028900_v2.0.a1 Prupe.1G029000_v2.0.a1 Prupe.1G029100_v2.0.a1
pyrus_communis pycom08g19680 pycom08g19730 pycom16g17460 pycom16g17500
rosa_chinensis RchiOBHm_Chr4g0389751 RchiOBHm_Chr4g0389771 RchiOBHm_Chr4g0389791 RchiOBHm_Chr4g0389861 RchiOBHm_Chr4g0389881 RchiOBHm_Chr4g0389901 RchiOBHm_Chr4g0412411
rosa_laevigata RLG00000008288 RLG00000009901 RLG00000009945 RLG00000009954 RLG00000009956 RLG00000009957 RLG00000009958 RLG00000019246
rosa_multiflora Rmu_co8275125.1_g000001 Rmu_sc0002083.1_g000003 Rmu_sc0002083.1_g000025 Rmu_sc0002604.1_g000016 Rmu_sc0004336.1_g000031 Rmu_sc0010423.1_g000003
rosa_roxburghii Rroxscaffold_5G00335790 Rroxscaffold_5G00335850 Rroxscaffold_5G00335970 Rroxscaffold_5G00335990
rosa_rugosa Rorug03G0325100 Rorug03G0325300 Rorug03G0325500.1 Rorug03G0326800 Rorug03G0327000 Rorug04G0104400
rosa_samantha Rh4AG029900 Rh4AG030000 Rh4AG030100 Rh4AG030200 Rh4AG030600 Rh4AG172900 Rh4BG023200 Rh4BG023900 Rh4BG024000 Rh4BG024100 Rh4BG172200 Rh4DG023300 Rh4DG023800 Rh4DG023900 Rh4DG024000 Rh4DG151600
rosa_wichuraiana Rw1G002330 Rw1G002340 Rw4G002260 Rw4G002270 Rw4G002280 Rw4G002300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 233
AccII CGCG 1 cut(s) 2036
AciI CCGC 6 cut(s) 44, 872, 1278, 1298, 1611, 1915
AclWI GGATC 8 cut(s) 647, 696, 1126, 1139, 1418, 1463, 1501, 1514
AcoI YGGCCR 1 cut(s) 1783
AcsI RAATTY 2 cut(s) 1626, 1859
AcuI CTGAAG 1 cut(s) 268
AcyI GRCGYC 1 cut(s) 1236
AdeI CACNNNGTG 1 cut(s) 344
AfaI GTAC 4 cut(s) 243, 881, 1205, 1472
AfiI CCNNNNNNNGG 3 cut(s) 1141, 1232, 1610
AflII CTTAAG 1 cut(s) 830
AflIII ACRYGT 1 cut(s) 244
AgeI ACCGGT 1 cut(s) 697
AgsI TTSAA 7 cut(s) 251, 934, 1267, 1567, 1804, 1859, 1870
AjiI CACGTC 1 cut(s) 136
AjnI CCWGG 2 cut(s) 340, 1451
AjuI GAANNNNNNNTTGG 4 cut(s) 266, 298, 1535, 1567
AleI CACNNNNGTG 1 cut(s) 1772
AloI GAACNNNNNNTCC 2 cut(s) 570, 602
Alw21I GWGCWC 3 cut(s) 81, 120, 302
Alw26I GTCTC 3 cut(s) 869, 1946, 1982
Alw44I GTGCAC 1 cut(s) 298
AlwI GGATC 8 cut(s) 647, 696, 1126, 1139, 1418, 1463, 1501, 1514
AlwNI CAGNNNCTG 1 cut(s) 1717
Ama87I CYCGRG 2 cut(s) 1631, 1927
AoxI GGCC 4 cut(s) 423, 936, 1188, 1783
ApaLI GTGCAC 1 cut(s) 298
ApeKI GCWGC 5 cut(s) 431, 964, 1717, 1911, 2010
ApoI RAATTY 2 cut(s) 1626, 1859
ArsI GACNNNNNNTTYG 4 cut(s) 137, 169, 570, 602
AsiGI ACCGGT 1 cut(s) 697
Asp700I GAANNNNTTC 1 cut(s) 1835
AspLEI GCGC 3 cut(s) 561, 1225, 2038
AspS9I GGNCC 5 cut(s) 547, 1188, 1764, 1807, 1945
AsuC2I CCSGG 1 cut(s) 1949
AsuHPI GGTGA 9 cut(s) 37, 46, 86, 356, 742, 1427, 1660, 1789, 1900
AvaI CYCGRG 2 cut(s) 1631, 1927
AvaII GGWCC 4 cut(s) 547, 1764, 1807, 1945
BaeGI GKGCMC 2 cut(s) 236, 302
BaeI ACNNNNGTAYC 2 cut(s) 827, 860
BalI TGGCCA 1 cut(s) 1785
BamHI GGATCC 2 cut(s) 1131, 1506
BanI GGYRCC 1 cut(s) 233
BanII GRGCYC 3 cut(s) 81, 120, 1604
BarI GAAGNNNNNNTAC 2 cut(s) 1463, 1495
BbsI GAAGAC 1 cut(s) 1623
Bbv12I GWGCWC 3 cut(s) 81, 120, 302
BbvCI CCTCAGC 2 cut(s) 890, 1013
BbvI GCAGC 5 cut(s) 443, 976, 1704, 1898, 1997
BccI CCATC 4 cut(s) 197, 971, 1737, 2036
BciT130I CCWGG 2 cut(s) 342, 1453
BciVI GTATCC 1 cut(s) 949
BclI TGATCA 1 cut(s) 591
BcnI CCSGG 1 cut(s) 1949
BcoDI GTCTC 3 cut(s) 869, 1946, 1982
BfaI CTAG 1 cut(s) 5
BfmI CTRYAG 1 cut(s) 1227
BfrI CTTAAG 1 cut(s) 830
BfuI GTATCC 1 cut(s) 949
BglI GCCNNNNNGGC 1 cut(s) 1608
BisI GCNGC 6 cut(s) 432, 965, 1718, 1912, 1915, 2011
BlpI GCTNAGC 2 cut(s) 60, 80
BlsI GCNGC 6 cut(s) 433, 966, 1719, 1913, 1916, 2012
Bme1390I CCNGG 3 cut(s) 342, 1453, 1949
Bme18I GGWCC 4 cut(s) 547, 1764, 1807, 1945
BmeT110I CYCGRG 2 cut(s) 1631, 1927
BmgBI CACGTC 1 cut(s) 136
BmgT120I GGNCC 5 cut(s) 547, 1188, 1764, 1807, 1945
BmiI GGNNCC 6 cut(s) 235, 464, 1133, 1508, 1765, 1947
BmrFI CCNGG 3 cut(s) 342, 1453, 1949
BmsI GCATC 2 cut(s) 630, 1185
BpiI GAAGAC 1 cut(s) 1623
BplI GAGNNNNNCTC 2 cut(s) 134, 166
BpmI CTGGAG 1 cut(s) 536
Bpu10I CCTNAGC 2 cut(s) 890, 1013
Bpu1102I GCTNAGC 2 cut(s) 60, 80
BpuEI CTTGAG 5 cut(s) 290, 500, 521, 653, 1204
BpuMI CCSGG 1 cut(s) 1949
BsaHI GRCGYC 1 cut(s) 1236
BsaI GGTCTC 1 cut(s) 1946
BsaJI CCNNGG 8 cut(s) 39, 369, 411, 543, 1199, 1621, 1786, 1983
BsaWI WCCGGW 2 cut(s) 697, 882
BsaXI ACNNNNNCTCC 2 cut(s) 2008, 2038
Bsc4I CCNNNNNNNGG 3 cut(s) 1141, 1232, 1610
Bse118I RCCGGY 2 cut(s) 697, 1232
Bse1I ACTGG 4 cut(s) 527, 762, 1085, 1508
Bse3DI GCAATG 4 cut(s) 602, 690, 1154, 1536
BseBI CCWGG 2 cut(s) 342, 1453
BseDI CCNNGG 8 cut(s) 39, 369, 411, 543, 1199, 1621, 1786, 1983
BseGI GGATG 4 cut(s) 667, 816, 1748, 1923
BseLI CCNNNNNNNGG 3 cut(s) 1141, 1232, 1610
BseMI GCAATG 4 cut(s) 602, 690, 1154, 1536
BseMII CTCAG 4 cut(s) 74, 94, 881, 1004
BseNI ACTGG 4 cut(s) 527, 762, 1085, 1508
BseRI GAGGAG 4 cut(s) 881, 1030, 1268, 1478
BseSI GKGCMC 2 cut(s) 236, 302
BseXI GCAGC 5 cut(s) 443, 976, 1704, 1898, 1997
BsgI GTGCAG 3 cut(s) 248, 983, 1232
Bsh1236I CGCG 1 cut(s) 2036
Bsh1285I CGRYCG 2 cut(s) 48, 702
BshFI GGCC 4 cut(s) 425, 938, 1190, 1785
BshNI GGYRCC 1 cut(s) 233
BshTI ACCGGT 1 cut(s) 697
BsiEI CGRYCG 2 cut(s) 48, 702
BsiHKAI GWGCWC 3 cut(s) 81, 120, 302
BsiHKCI CYCGRG 2 cut(s) 1631, 1927
BsiSI CCGG 4 cut(s) 698, 883, 1233, 1949
BslFI GGGAC 3 cut(s) 775, 1111, 1931
BslI CCNNNNNNNGG 3 cut(s) 1141, 1232, 1610
BsmAI GTCTC 3 cut(s) 869, 1946, 1982
BsmBI CGTCTC 1 cut(s) 869
BsmFI GGGAC 3 cut(s) 775, 1111, 1931
BsmI GAATGC 2 cut(s) 199, 1327
BsnI GGCC 4 cut(s) 425, 938, 1190, 1785
Bso31I GGTCTC 1 cut(s) 1946
BsoBI CYCGRG 2 cut(s) 1631, 1927
Bsp1286I GDGCHC 5 cut(s) 81, 120, 236, 302, 1604
Bsp1720I GCTNAGC 2 cut(s) 60, 80
Bsp19I CCATGG 3 cut(s) 1199, 1786, 1983
BspACI CCGC 6 cut(s) 44, 872, 1278, 1298, 1611, 1915
BspANI GGCC 4 cut(s) 425, 938, 1190, 1785
BspCNI CTCAG 4 cut(s) 73, 93, 882, 1005
BspFNI CGCG 1 cut(s) 2036
BspLI GGNNCC 6 cut(s) 235, 464, 1133, 1508, 1765, 1947
BspPI GGATC 8 cut(s) 647, 696, 1126, 1139, 1418, 1463, 1501, 1514
BspT107I GGYRCC 1 cut(s) 233
BspTI CTTAAG 1 cut(s) 830
BspTNI GGTCTC 1 cut(s) 1946
BsrDI GCAATG 4 cut(s) 602, 690, 1154, 1536
BsrFI RCCGGY 2 cut(s) 697, 1232
BsrI ACTGG 4 cut(s) 527, 762, 1085, 1508
BssAI RCCGGY 2 cut(s) 697, 1232
BssECI CCNNGG 8 cut(s) 39, 369, 411, 543, 1199, 1621, 1786, 1983
BssNI GRCGYC 1 cut(s) 1236
BssT1I CCWWGG 6 cut(s) 369, 411, 1199, 1621, 1786, 1983
Bst2UI CCWGG 2 cut(s) 342, 1453
Bst4CI ACNGT 5 cut(s) 961, 997, 1210, 1696, 1771
Bst6I CTCTTC 3 cut(s) 92, 176, 765
BstACI GRCGYC 1 cut(s) 1236
BstAFI CTTAAG 1 cut(s) 830
BstAPI GCANNNNNTGC 1 cut(s) 437
BstC8I GCNNGC 8 cut(s) 335, 423, 563, 567, 669, 1234, 1274, 1278
BstDEI CTNAG 8 cut(s) 60, 80, 498, 744, 890, 951, 1013, 1758
BstDSI CCRYGG 4 cut(s) 543, 1199, 1786, 1983
BstEII GGTNACC 1 cut(s) 34
BstF5I GGATG 4 cut(s) 667, 816, 1748, 1923
BstFNI CGCG 1 cut(s) 2036
BstHHI GCGC 3 cut(s) 561, 1225, 2038
BstMAI GTCTC 3 cut(s) 869, 1946, 1982
BstMCI CGRYCG 2 cut(s) 48, 702
BstMWI GCNNNNNNNGC 8 cut(s) 56, 422, 431, 437, 637, 1229, 1608, 2007
BstNI CCWGG 2 cut(s) 342, 1453
BstNSI RCATGY 1 cut(s) 248
BstPI GGTNACC 1 cut(s) 34
BstSCI CCNGG 3 cut(s) 340, 1451, 1947
BstSFI CTRYAG 1 cut(s) 1227
BstSLI GKGCMC 2 cut(s) 236, 302
BstUI CGCG 1 cut(s) 2036
BstV1I GCAGC 5 cut(s) 443, 976, 1704, 1898, 1997
BstV2I GAAGAC 1 cut(s) 1623
BstX2I RGATCY 2 cut(s) 1131, 1506
BstXI CCANNNNNNTGG 1 cut(s) 1774
BstYI RGATCY 2 cut(s) 1131, 1506
BsuI GTATCC 1 cut(s) 949
BsuRI GGCC 4 cut(s) 425, 938, 1190, 1785
BtgI CCRYGG 4 cut(s) 543, 1199, 1786, 1983
BtrI CACGTC 1 cut(s) 136
BtsCI GGATG 4 cut(s) 667, 816, 1748, 1923
BtsI GCAGTG 3 cut(s) 54, 423, 1906
BtsIMutI CAGTG 9 cut(s) 54, 180, 423, 769, 1206, 1692, 1767, 1906, 2016
Cac8I GCNNGC 8 cut(s) 335, 423, 563, 567, 669, 1234, 1274, 1278
CaiI CAGNNNCTG 1 cut(s) 1717
CfoI GCGC 3 cut(s) 561, 1225, 2038
Cfr10I RCCGGY 2 cut(s) 697, 1232
Cfr13I GGNCC 5 cut(s) 547, 1188, 1764, 1807, 1945
CseI GACGC 1 cut(s) 1225
CsiI ACCWGGT 1 cut(s) 340
Csp6I GTAC 4 cut(s) 242, 880, 1204, 1471
CspAI ACCGGT 1 cut(s) 697
CviQI GTAC 4 cut(s) 242, 880, 1204, 1471
DdeI CTNAG 8 cut(s) 60, 80, 498, 744, 890, 951, 1013, 1758
DraIII CACNNNGTG 1 cut(s) 344
EaeI YGGCCR 1 cut(s) 1783
Eam1104I CTCTTC 3 cut(s) 92, 176, 765
EarI CTCTTC 3 cut(s) 92, 176, 765
EciI GGCGGA 2 cut(s) 887, 1626
Ecl136II GAGCTC 2 cut(s) 79, 118
Eco130I CCWWGG 6 cut(s) 369, 411, 1199, 1621, 1786, 1983
Eco147I AGGCCT 1 cut(s) 938
Eco24I GRGCYC 3 cut(s) 81, 120, 1604
Eco31I GGTCTC 1 cut(s) 1946
Eco47I GGWCC 4 cut(s) 547, 1764, 1807, 1945
Eco53kI GAGCTC 2 cut(s) 79, 118
Eco57I CTGAAG 1 cut(s) 268
Eco88I CYCGRG 2 cut(s) 1631, 1927
Eco91I GGTNACC 1 cut(s) 34
EcoICRI GAGCTC 2 cut(s) 79, 118
EcoO109I RGGNCCY 2 cut(s) 1807, 1945
EcoO65I GGTNACC 1 cut(s) 34
EcoRI GAATTC 2 cut(s) 1626, 1859
EcoRII CCWGG 2 cut(s) 340, 1451
EcoT14I CCWWGG 6 cut(s) 369, 411, 1199, 1621, 1786, 1983
EcoT38I GRGCYC 3 cut(s) 81, 120, 1604
ErhI CCWWGG 6 cut(s) 369, 411, 1199, 1621, 1786, 1983
Esp3I CGTCTC 1 cut(s) 869
FalI AAGNNNNNCTT 4 cut(s) 353, 385, 1460, 1492
FaqI GGGAC 3 cut(s) 775, 1111, 1931
FauI CCCGC 2 cut(s) 1285, 1305
FauNDI CATATG 1 cut(s) 781
FbaI TGATCA 1 cut(s) 591
Fnu4HI GCNGC 6 cut(s) 432, 965, 1718, 1912, 1915, 2011
FokI GGATG 4 cut(s) 674, 803, 1755, 1910
FriOI GRGCYC 3 cut(s) 81, 120, 1604
Fsp4HI GCNGC 6 cut(s) 432, 965, 1718, 1912, 1915, 2011
FspBI CTAG 1 cut(s) 5
GlaI GCGC 3 cut(s) 560, 1224, 2037
GluI GCNGC 6 cut(s) 432, 965, 1718, 1912, 1915, 2011
GsuI CTGGAG 1 cut(s) 536
HaeIII GGCC 4 cut(s) 425, 938, 1190, 1785
HapII CCGG 4 cut(s) 698, 883, 1233, 1949
HgaI GACGC 1 cut(s) 1225
HhaI GCGC 3 cut(s) 561, 1225, 2038
Hin1I GRCGYC 1 cut(s) 1236
Hin6I GCGC 3 cut(s) 559, 1223, 2036
HinP1I GCGC 3 cut(s) 559, 1223, 2036
HincII GTYRAC 2 cut(s) 366, 1678
HindII GTYRAC 2 cut(s) 366, 1678
HindIII AAGCTT 2 cut(s) 638, 827
HinfI GANTC 7 cut(s) 149, 164, 1242, 1336, 1342, 1891, 2021
HpaII CCGG 4 cut(s) 698, 883, 1233, 1949
HphI GGTGA 9 cut(s) 37, 46, 86, 356, 742, 1427, 1660, 1789, 1900
Hpy166II GTNNAC 6 cut(s) 300, 366, 1206, 1518, 1678, 1767
Hpy188I TCNGA 6 cut(s) 100, 906, 1060, 1241, 1341, 1896
Hpy8I GTNNAC 6 cut(s) 300, 366, 1206, 1518, 1678, 1767
Hpy99I CGWCG 1 cut(s) 509
HpyAV CCTTC 8 cut(s) 292, 542, 764, 1348, 1561, 1798, 1843, 1879
HpyCH4III ACNGT 5 cut(s) 961, 997, 1210, 1696, 1771
HpyCH4IV ACGT 3 cut(s) 135, 585, 878
HpyF10VI GCNNNNNNNGC 8 cut(s) 56, 422, 431, 437, 637, 1229, 1608, 2007
HpyF3I CTNAG 8 cut(s) 60, 80, 498, 744, 890, 951, 1013, 1758
HpySE526I ACGT 3 cut(s) 135, 585, 878
Hsp92I GRCGYC 1 cut(s) 1236
HspAI GCGC 3 cut(s) 559, 1223, 2036
KroI GCCGGC 1 cut(s) 1232
KroNI GCCGGC 1 cut(s) 1234
Ksp22I TGATCA 1 cut(s) 591
LmnI GCTCC 2 cut(s) 468, 1144
Lsp1109I GCAGC 5 cut(s) 443, 976, 1704, 1898, 1997
LweI GCATC 2 cut(s) 630, 1185
MabI ACCWGGT 1 cut(s) 340
MaeI CTAG 1 cut(s) 5
MaeII ACGT 3 cut(s) 135, 585, 878
MaeIII GTNAC 4 cut(s) 34, 178, 485, 1696
MfeI CAATTG 2 cut(s) 678, 839
MflI RGATCY 2 cut(s) 1131, 1506
MhlI GDGCHC 5 cut(s) 81, 120, 236, 302, 1604
MlsI TGGCCA 1 cut(s) 1785
MluCI AATT 9 cut(s) 351, 678, 794, 839, 969, 1562, 1626, 1650, 1859
MluNI TGGCCA 1 cut(s) 1785
MlyI GAGTC 1 cut(s) 143
MmeI TCCRAC 1 cut(s) 123
Mox20I TGGCCA 1 cut(s) 1785
MroNI GCCGGC 1 cut(s) 1232
MroXI GAANNNNTTC 1 cut(s) 1835
MscI TGGCCA 1 cut(s) 1785
MseI TTAA 2 cut(s) 831, 845
MslI CAYNNNNRTG 3 cut(s) 138, 1007, 1772
Msp20I TGGCCA 1 cut(s) 1785
MspA1I CMGCKG 1 cut(s) 1280
MspCI CTTAAG 1 cut(s) 830
MspI CCGG 4 cut(s) 698, 883, 1233, 1949
MspR9I CCNGG 3 cut(s) 342, 1453, 1949
MunI CAATTG 2 cut(s) 678, 839
Mva1269I GAATGC 2 cut(s) 199, 1327
MvaI CCWGG 2 cut(s) 342, 1453
MvnI CGCG 1 cut(s) 2036
MwoI GCNNNNNNNGC 8 cut(s) 56, 422, 431, 437, 637, 1229, 1608, 2007
NaeI GCCGGC 1 cut(s) 1234
NciI CCSGG 1 cut(s) 1949
NcoI CCATGG 3 cut(s) 1199, 1786, 1983
NdeI CATATG 1 cut(s) 781
NgoMIV GCCGGC 1 cut(s) 1232
NlaIV GGNNCC 6 cut(s) 235, 464, 1133, 1508, 1765, 1947
NmeAIII GCCGAG 1 cut(s) 20
NmuCI GTSAC 4 cut(s) 34, 178, 485, 1696
NspI RCATGY 1 cut(s) 248
OliI CACNNNNGTG 1 cut(s) 1772
PceI AGGCCT 1 cut(s) 938
PciI ACATGT 1 cut(s) 244
PcsI WCGNNNNNNNCGW 1 cut(s) 582
PctI GAATGC 2 cut(s) 199, 1327
PdiI GCCGGC 1 cut(s) 1234
PdmI GAANNNNTTC 1 cut(s) 1835
PfeI GAWTC 6 cut(s) 164, 1242, 1336, 1342, 1891, 2021
PfoI TCCNGGA 1 cut(s) 1947
PinAI ACCGGT 1 cut(s) 697
PkrI GCNGC 6 cut(s) 433, 966, 1719, 1913, 1916, 2012
PleI GAGTC 1 cut(s) 143
PpsI GAGTC 1 cut(s) 143
PpuMI RGGWCCY 2 cut(s) 1807, 1945
PscI ACATGT 1 cut(s) 244
Psp124BI GAGCTC 2 cut(s) 81, 120
Psp5II RGGWCCY 2 cut(s) 1807, 1945
Psp6I CCWGG 2 cut(s) 340, 1451
PspEI GGTNACC 1 cut(s) 34
PspGI CCWGG 2 cut(s) 340, 1451
PspN4I GGNNCC 6 cut(s) 235, 464, 1133, 1508, 1765, 1947
PspPI GGNCC 5 cut(s) 547, 1188, 1764, 1807, 1945
PspPPI RGGWCCY 2 cut(s) 1807, 1945
PstNI CAGNNNCTG 1 cut(s) 1717
PsuI RGATCY 2 cut(s) 1131, 1506
RsaI GTAC 4 cut(s) 243, 881, 1205, 1472
RsaNI GTAC 4 cut(s) 242, 880, 1204, 1471
RseI CAYNNNNRTG 3 cut(s) 138, 1007, 1772
SacI GAGCTC 2 cut(s) 81, 120
SaqAI TTAA 2 cut(s) 831, 845
SatI GCNGC 6 cut(s) 432, 965, 1718, 1912, 1915, 2011
Sau96I GGNCC 5 cut(s) 547, 1188, 1764, 1807, 1945
SchI GAGTC 1 cut(s) 143
ScrFI CCNGG 3 cut(s) 342, 1453, 1949
SduI GDGCHC 5 cut(s) 81, 120, 236, 302, 1604
SexAI ACCWGGT 1 cut(s) 340
SfaNI GCATC 2 cut(s) 630, 1185
SfcI CTRYAG 1 cut(s) 1227
SinI GGWCC 4 cut(s) 547, 1764, 1807, 1945
SmiMI CAYNNNNRTG 3 cut(s) 138, 1007, 1772
SmlI CTYRAG 6 cut(s) 305, 515, 536, 632, 830, 1183
SmoI CTYRAG 6 cut(s) 305, 515, 536, 632, 830, 1183
Sse9I AATT 9 cut(s) 351, 678, 794, 839, 969, 1562, 1626, 1650, 1859
SseBI AGGCCT 1 cut(s) 938
SsiI CCGC 6 cut(s) 44, 872, 1278, 1298, 1611, 1915
SspI AATATT 2 cut(s) 111, 1576
SspMI CTAG 1 cut(s) 5
SstI GAGCTC 2 cut(s) 81, 120
StuI AGGCCT 1 cut(s) 938
StyD4I CCNGG 3 cut(s) 340, 1451, 1947
StyI CCWWGG 6 cut(s) 369, 411, 1199, 1621, 1786, 1983
TaaI ACNGT 5 cut(s) 961, 997, 1210, 1696, 1771
TaiI ACGT 3 cut(s) 138, 588, 881
TaqI TCGA 7 cut(s) 203, 576, 702, 983, 1073, 1176, 1821
TasI AATT 9 cut(s) 351, 678, 794, 839, 969, 1562, 1626, 1650, 1859
TatI WGTACW 2 cut(s) 241, 1470
TauI GCSGC 1 cut(s) 1917
TfiI GAWTC 6 cut(s) 164, 1242, 1336, 1342, 1891, 2021
Tru1I TTAA 2 cut(s) 831, 845
Tru9I TTAA 2 cut(s) 831, 845
TscAI CASTG 9 cut(s) 61, 187, 423, 769, 1213, 1699, 1774, 1913, 2023
TseFI GTSAC 4 cut(s) 34, 178, 485, 1696
TseI GCWGC 5 cut(s) 431, 964, 1717, 1911, 2010
Tsp45I GTSAC 4 cut(s) 34, 178, 485, 1696
TspGWI ACGGA 2 cut(s) 493, 560
TspRI CASTG 9 cut(s) 61, 187, 423, 769, 1213, 1699, 1774, 1913, 2023
Vha464I CTTAAG 1 cut(s) 830
VneI GTGCAC 1 cut(s) 298
VpaK11BI GGWCC 4 cut(s) 547, 1764, 1807, 1945
XapI RAATTY 2 cut(s) 1626, 1859
XceI RCATGY 1 cut(s) 248
XcmI CCANNNNNNNNNTGG 1 cut(s) 418
XmnI GAANNNNTTC 1 cut(s) 1835
XspI CTAG 1 cut(s) 5
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.