pycom08g19730

Sieve element occlusion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0000511
Physical Location & Seq
Forward (+)
19714681 .. 19718669
3989 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g19730.10

Sequence Viewer

Length: 2100 bp
ATGGAGACCCTGATTTGGACCCTCGTGACTGAAAAGGTTCGATCATTCGACCGAATAAAAAAAAAAAGTCCATCTTCGTTGGTCATGGAAAGCCTACCAGAAAATGAGGTCATGACCGAGGAGTTGTCTGTCCAACTACTAACCGATGATGAGCAAGAGCAACGCATGGAGATGGGGGTTAGTGATCAGGGTGAGGGTGAGGGTGAAGGTGAAGGTAGTGAACGGATCAGCCTTTTCGACAGGTCTCAAAACGAAATCATGAAGCTCATTTTTGCAGTTAATTACCGTGTTCCTGATGAGGGCCATGCCAGCTTTGATGTTCATGCTATTTTCTCGACTAGTCAGAAAATCATTAAGGATTCGACCAAGATCATACACAATATTATTGAGCAGGGCAGCGGATTTAATGATGTTGAAACACCCAAAGCCAGCTCCAGCTTCTGCACTCTCATTAACTCCTTTGGCTGCAAGATGTCGTGTAAGAGTCCAGGTGACGAAAATGCATTTGAATCACGTACGGAAGAAATACTTATAAAACTATCAAGTTATCCATGTGAAGTACATGTAGTCTTGGCCCTGGCCGTTTTTGCTTTGGAATATGAAGAATGTTCCAGCCAACTTTCCAAGAAATCGACATCGCTGAAACGCCAAGATGCAGTAGTTGAGCTTAACAATCTGGTCAACCAAGCATTACAAGTGATTGAGCACATCCTTGAGTTGGAGAAACTAATTTCTGGTGATCGAGACAGTGAACCAAAATTGGCGAGGGTAATTATGGATACCCCATTTTATGCCTACTGGTCAATCATAACTCTTGTAGCTTGCGCAACTGAGCTCTCTCTTCTTACCAGTGATGAGGACATTAAGTCATATGATCTATCCCATTTTTCTCACAAAATGGGCTTCATCCTCAACAAGTTTGAGGGACAGCTTACAATCTGCCAACAAGAAAAAGAGGAGGTAAGGGCCAAGAAAGAGCTTTGGAAACTTGTATGCACTGCCCCTCCTAAAATTACCGAGGTTTTCCAAAGGCTGGTTCTTTTCAAGCATAAAGCGTATCCGCAGCCTCCCATCATCAGTGTTAATCCCACTAACAAGGAGGTTTTTAACATCAATGTGCTAGAGGGGAAGTGGGTGTTATTTTACATTTCAAGCCTGGACAATGTTTCTGATCAAGATATTTCATGTCTCAAAGAAGTGTACGAGGGAATCGAAAAGCATAACAAGTGTGTGATTGTGTGGATCCCTGTCGTTGAGGACTGGACGGGAGGAGGAAAAGAGCAGTTTAAGGAGTGGAGGTCTAAGATGCCATGGTACGCGGTGCAATATTTTTCGCCCACGGTGATTGGGTACCTTGAGCAGCGGTGGAACTTCAAAGGTAATCCCATGGTGGTGGTGATGAACCGGAAAGGAACGGTGGCAATCGGAAACATAATCGACTTGATTCGGACACAAGCAACGGAGGCAATCGCTTTGCTTAACGTTGAAATACAAAAAGAAATACGGGAGGAGGAGGAGCACTCTGCCTGGTTTGGAACGACTGCTGCGGCAATCGCTTTGCTTTTCGCTGAAATACCAGAAGCACGGGAGTGCCGTGCCAAAGGCTTGTCTCGAACGCTTGCTGAAGCTGTGGTGGTGTTGAAGAGTTTAACTCGTCCTCAGCCATTCACTAATACTGATGCGACGCCTGTCCTTCTTGATGGTTCTAACAAGCAGGTTAATATCGACAATGAGCTGAAGTGGAAGGATATGTTTTTGTTACTCTCCGATGTAGGCATTGAAGATTGTTACATTTCATATCTGAAACCAGTTTATGAGGGAATAAGAAGATACCCATCGTATCGGATTGTCTGGGTTCCCGTTGTGGAGCAGTGGAACCAAGACAAAGAAAAACAACTTGAGATGTCGAGGTTGAAGATGCCGTGGTACACATTGAAGTGTTTTCCAACCAAACCAGGCATCAAGTACATGAAAGAGAAGTGGAATTACAAGGGTAAGCCTGCGGTGGTGGTGATGACCAGTGCAGGTATGGTGAAAAATAAAAATGCTTTCCCCTTGATTAAGAAAAATGGAATGGACGCCTTTCCTTTCTTTAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SEO_C SEO_N

Protein Analysis

700

Amino Acids

79.78

Weight (kDa)

5.56

Isoelectric Point (pI)

50.23

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000511)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g02370 FvH4_4g02370 FvH4_4g02370 FvH4_4g02430 FvH4_4g02430 FvH4_4g02460 FvH4_4g02460 FvH4_4g02460 FvH4_4g02480 FvH4_4g02480 FvH4_4g02480 FvH4_4g02480 FvH4_6g05920
malus_domestica MD00G1111000.v1.1 MD08G1225800.v1.1 MD13G1205700.v1.1 MD13G1205800.v1.1 MD13G1205900.v1.1 MD13G1206000.v1.1 MD16G1207500.v1.1 MD16G1207600.v1.1
prunus_persica Prupe.1G028400_v2.0.a1 Prupe.1G028500_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028900_v2.0.a1 Prupe.1G029000_v2.0.a1 Prupe.1G029100_v2.0.a1
pyrus_communis pycom08g19680 pycom08g19730 pycom16g17460 pycom16g17500
rosa_chinensis RchiOBHm_Chr4g0389751 RchiOBHm_Chr4g0389771 RchiOBHm_Chr4g0389791 RchiOBHm_Chr4g0389861 RchiOBHm_Chr4g0389881 RchiOBHm_Chr4g0389901 RchiOBHm_Chr4g0412411
rosa_laevigata RLG00000008288 RLG00000009901 RLG00000009945 RLG00000009954 RLG00000009956 RLG00000009957 RLG00000009958 RLG00000019246
rosa_multiflora Rmu_co8275125.1_g000001 Rmu_sc0002083.1_g000003 Rmu_sc0002083.1_g000025 Rmu_sc0002604.1_g000016 Rmu_sc0004336.1_g000031 Rmu_sc0010423.1_g000003
rosa_roxburghii Rroxscaffold_5G00335790 Rroxscaffold_5G00335850 Rroxscaffold_5G00335970 Rroxscaffold_5G00335990
rosa_rugosa Rorug03G0325100 Rorug03G0325300 Rorug03G0325500.1 Rorug03G0326800 Rorug03G0327000 Rorug04G0104400
rosa_samantha Rh4AG029900 Rh4AG030000 Rh4AG030100 Rh4AG030200 Rh4AG030600 Rh4AG172900 Rh4BG023200 Rh4BG023900 Rh4BG024000 Rh4BG024100 Rh4BG172200 Rh4DG023300 Rh4DG023800 Rh4DG023900 Rh4DG024000 Rh4DG151600
rosa_wichuraiana Rw1G002330 Rw1G002340 Rw4G002260 Rw4G002270 Rw4G002280 Rw4G002300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 533
Acc16I TGCGCA 1 cut(s) 826
Acc36I ACCTGC 2 cut(s) 1705, 2015
Acc65I GGTACC 1 cut(s) 1350
AccB1I GGYRCC 1 cut(s) 1350
AccB7I CCANNNNNTGG 1 cut(s) 1033
AccII CGCG 1 cut(s) 1319
AciI CCGC 6 cut(s) 399, 1061, 1319, 1363, 1547, 2003
AclI AACGTT 1 cut(s) 1482
AclWI GGATC 3 cut(s) 233, 1237, 1250
AcoI YGGCCR 1 cut(s) 579
AcuI CTGAAG 2 cut(s) 1644, 1757
AcyI GRCGYC 2 cut(s) 1685, 2079
AfaI GTAC 7 cut(s) 517, 561, 1202, 1316, 1352, 1928, 1967
AfiI CCNNNNNNNGG 4 cut(s) 15, 299, 718, 1033
AflIII ACRYGT 1 cut(s) 562
AhdI GACNNNNNGTC 1 cut(s) 865
AhlI ACTAGT 1 cut(s) 338
AjnI CCWGG 5 cut(s) 487, 576, 1155, 1526, 1954
AjuI GAANNNNNNNTTGG 2 cut(s) 1020, 1052
AleI CACNNNNGTG 1 cut(s) 1588
Alw21I GWGCWC 3 cut(s) 708, 837, 1521
Alw26I GTCTC 4 cut(s) 249, 738, 1193, 1614
AlwI GGATC 3 cut(s) 233, 1237, 1250
AlwNI CAGNNNCTG 1 cut(s) 441
AoxI GGCC 4 cut(s) 301, 573, 579, 966
ApeKI GCWGC 5 cut(s) 396, 465, 1063, 1360, 1544
Asp700I GAANNNNTTC 1 cut(s) 36
Asp718I GGTACC 1 cut(s) 1350
AspLEI GCGC 1 cut(s) 827
AspS9I GGNCC 4 cut(s) 18, 301, 574, 966
AvaII GGWCC 1 cut(s) 18
BamHI GGATCC 1 cut(s) 1242
BanI GGYRCC 1 cut(s) 1350
BanII GRGCYC 1 cut(s) 837
BarI GAAGNNNNNNTAC 2 cut(s) 1970, 2002
BauI CACGAG 1 cut(s) 23
Bbv12I GWGCWC 3 cut(s) 708, 837, 1521
BbvCI CCTCAGC 1 cut(s) 1659
BbvI GCAGC 5 cut(s) 408, 452, 1075, 1372, 1531
BccI CCATC 5 cut(s) 79, 166, 1079, 1694, 1843
BceAI ACGGC 3 cut(s) 566, 1578, 1906
BciT130I CCWGG 5 cut(s) 489, 578, 1157, 1528, 1956
BciVI GTATCC 2 cut(s) 772, 1068
BclI TGATCA 2 cut(s) 184, 1171
BcoDI GTCTC 4 cut(s) 249, 738, 1193, 1614
BcuI ACTAGT 1 cut(s) 338
BfaI CTAG 2 cut(s) 339, 1121
BfuAI ACCTGC 2 cut(s) 1705, 2015
BfuI GTATCC 2 cut(s) 772, 1068
BisI GCNGC 6 cut(s) 397, 466, 1064, 1361, 1545, 1548
BlsI GCNGC 6 cut(s) 398, 467, 1065, 1362, 1546, 1549
Bme1390I CCNGG 5 cut(s) 489, 578, 1157, 1528, 1956
Bme18I GGWCC 1 cut(s) 18
BmeRI GACNNNNNGTC 1 cut(s) 865
BmgT120I GGNCC 4 cut(s) 18, 301, 574, 966
BmiI GGNNCC 5 cut(s) 20, 1244, 1352, 1857, 1877
BmrFI CCNGG 5 cut(s) 489, 578, 1157, 1528, 1956
BmsI GCATC 5 cut(s) 643, 1296, 1669, 1908, 1968
BoxI GACNNNNGTC 1 cut(s) 1688
BplI GAGNNNNNCTC 4 cut(s) 1505, 1537, 1636, 1668
BpmI CTGGAG 1 cut(s) 418
Bpu10I CCTNAGC 1 cut(s) 1659
BpuEI CTTGAG 3 cut(s) 734, 1376, 1919
BsaAI YACGTR 1 cut(s) 515
BsaBI GATNNNNATC 1 cut(s) 1834
BsaHI GRCGYC 2 cut(s) 1685, 2079
BsaI GGTCTC 1 cut(s) 249
BsaJI CCNNGG 7 cut(s) 117, 576, 1017, 1310, 1338, 1386, 1922
BsaWI WCCGGW 1 cut(s) 1404
BsaXI ACNNNNNCTCC 4 cut(s) 988, 1018, 1283, 1313
Bsc4I CCNNNNNNNGG 4 cut(s) 15, 299, 718, 1033
Bse1I ACTGG 5 cut(s) 803, 849, 1265, 1808, 2019
Bse8I GATNNNNATC 1 cut(s) 1834
BseBI CCWGG 5 cut(s) 489, 578, 1157, 1528, 1956
BseDI CCNNGG 7 cut(s) 117, 576, 1017, 1310, 1338, 1386, 1922
BseGI GGATG 2 cut(s) 708, 906
BseJI GATNNNNATC 1 cut(s) 1834
BseLI CCNNNNNNNGG 4 cut(s) 15, 299, 718, 1033
BseMII CTCAG 2 cut(s) 822, 1673
BseNI ACTGG 5 cut(s) 803, 849, 1265, 1808, 2019
BseRI GAGGAG 6 cut(s) 134, 971, 1284, 1523, 1526, 1529
BseXI GCAGC 5 cut(s) 408, 452, 1075, 1372, 1531
BsgI GTGCAG 2 cut(s) 427, 2043
Bsh1236I CGCG 1 cut(s) 1319
Bsh1285I CGRYCG 1 cut(s) 52
BshFI GGCC 4 cut(s) 303, 575, 581, 968
BshNI GGYRCC 1 cut(s) 1350
BsiEI CGRYCG 1 cut(s) 52
BsiHKAI GWGCWC 3 cut(s) 708, 837, 1521
BsiSI CCGG 1 cut(s) 1405
BsiWI CGTACG 1 cut(s) 515
BslFI GGGAC 1 cut(s) 939
BslI CCNNNNNNNGG 4 cut(s) 15, 299, 718, 1033
BsmAI GTCTC 4 cut(s) 249, 738, 1193, 1614
BsmFI GGGAC 1 cut(s) 939
BsnI GGCC 4 cut(s) 303, 575, 581, 968
Bso31I GGTCTC 1 cut(s) 249
Bsp1286I GDGCHC 3 cut(s) 708, 837, 1521
Bsp143I GATC 8 cut(s) 41, 184, 225, 369, 739, 874, 1171, 1242
Bsp19I CCATGG 2 cut(s) 1310, 1386
BspACI CCGC 6 cut(s) 399, 1061, 1319, 1363, 1547, 2003
BspANI GGCC 4 cut(s) 303, 575, 581, 968
BspCNI CTCAG 2 cut(s) 823, 1672
BspFNI CGCG 1 cut(s) 1319
BspHI TCATGA 2 cut(s) 111, 258
BspLI GGNNCC 5 cut(s) 20, 1244, 1352, 1857, 1877
BspMI ACCTGC 2 cut(s) 1705, 2015
BspPI GGATC 3 cut(s) 233, 1237, 1250
BspT107I GGYRCC 1 cut(s) 1350
BspTNI GGTCTC 1 cut(s) 249
BsrI ACTGG 5 cut(s) 803, 849, 1265, 1808, 2019
BssECI CCNNGG 7 cut(s) 117, 576, 1017, 1310, 1338, 1386, 1922
BssMI GATC 8 cut(s) 41, 184, 225, 369, 739, 874, 1171, 1242
BssNI GRCGYC 2 cut(s) 1685, 2079
BssSI CACGAG 1 cut(s) 23
BssT1I CCWWGG 2 cut(s) 1310, 1386
Bst2BI CACGAG 1 cut(s) 23
Bst2UI CCWGG 5 cut(s) 489, 578, 1157, 1528, 1956
Bst4CI ACNGT 4 cut(s) 287, 749, 1342, 1417
Bst6I CTCTTC 2 cut(s) 846, 1637
BstACI GRCGYC 2 cut(s) 1685, 2079
BstBAI YACGTR 1 cut(s) 515
BstC8I GCNNGC 5 cut(s) 310, 430, 823, 1620, 2001
BstDEI CTNAG 3 cut(s) 831, 1302, 1659
BstDSI CCRYGG 4 cut(s) 1310, 1338, 1386, 1922
BstF5I GGATG 2 cut(s) 708, 906
BstFNI CGCG 1 cut(s) 1319
BstHHI GCGC 1 cut(s) 827
BstKTI GATC 8 cut(s) 44, 187, 228, 372, 742, 877, 1174, 1245
BstMAI GTCTC 4 cut(s) 249, 738, 1193, 1614
BstMBI GATC 8 cut(s) 41, 184, 225, 369, 739, 874, 1171, 1242
BstMCI CGRYCG 1 cut(s) 52
BstMWI GCNNNNNNNGC 4 cut(s) 309, 587, 1463, 1553
BstNI CCWGG 5 cut(s) 489, 578, 1157, 1528, 1956
BstNSI RCATGY 1 cut(s) 566
BstPAI GACNNNNGTC 1 cut(s) 1688
BstSCI CCNGG 5 cut(s) 487, 576, 1155, 1526, 1954
BstUI CGCG 1 cut(s) 1319
BstV1I GCAGC 5 cut(s) 408, 452, 1075, 1372, 1531
BstX2I RGATCY 1 cut(s) 1242
BstXI CCANNNNNNTGG 1 cut(s) 1393
BstYI RGATCY 1 cut(s) 1242
BsuI GTATCC 2 cut(s) 772, 1068
BsuRI GGCC 4 cut(s) 303, 575, 581, 968
BtgI CCRYGG 4 cut(s) 1310, 1338, 1386, 1922
BtgZI GCGATG 1 cut(s) 621
BtsCI GGATG 2 cut(s) 708, 906
BtsI GCAGTG 2 cut(s) 996, 1877
BtsIMutI CAGTG 6 cut(s) 754, 856, 996, 1084, 1877, 2026
BveI ACCTGC 2 cut(s) 1705, 2015
Cac8I GCNNGC 5 cut(s) 310, 430, 823, 1620, 2001
CaiI CAGNNNCTG 1 cut(s) 441
CciI TCATGA 2 cut(s) 111, 258
CfoI GCGC 1 cut(s) 827
Cfr13I GGNCC 4 cut(s) 18, 301, 574, 966
CseI GACGC 2 cut(s) 1693, 2087
Csp6I GTAC 7 cut(s) 516, 560, 1201, 1315, 1351, 1927, 1966
CspCI CAANNNNNGTGG 2 cut(s) 1327, 1362
CviQI GTAC 7 cut(s) 516, 560, 1201, 1315, 1351, 1927, 1966
DdeI CTNAG 3 cut(s) 831, 1302, 1659
DpnI GATC 8 cut(s) 43, 186, 227, 371, 741, 876, 1173, 1244
DpnII GATC 8 cut(s) 41, 184, 225, 369, 739, 874, 1171, 1242
DraI TTTAAA 1 cut(s) 2095
DriI GACNNNNNGTC 1 cut(s) 865
EaeI YGGCCR 1 cut(s) 579
Eam1104I CTCTTC 2 cut(s) 846, 1637
Eam1105I GACNNNNNGTC 1 cut(s) 865
EarI CTCTTC 2 cut(s) 846, 1637
Ecl136II GAGCTC 1 cut(s) 835
Eco130I CCWWGG 2 cut(s) 1310, 1386
Eco24I GRGCYC 1 cut(s) 837
Eco31I GGTCTC 1 cut(s) 249
Eco47I GGWCC 1 cut(s) 18
Eco53kI GAGCTC 1 cut(s) 835
Eco57I CTGAAG 2 cut(s) 1644, 1757
EcoICRI GAGCTC 1 cut(s) 835
EcoRII CCWGG 5 cut(s) 487, 576, 1155, 1526, 1954
EcoT14I CCWWGG 2 cut(s) 1310, 1386
EcoT22I ATGCAT 1 cut(s) 505
EcoT38I GRGCYC 1 cut(s) 837
ErhI CCWWGG 2 cut(s) 1310, 1386
FalI AAGNNNNNCTT 4 cut(s) 58, 90, 513, 545
FaqI GGGAC 1 cut(s) 939
FauNDI CATATG 1 cut(s) 871
FbaI TGATCA 2 cut(s) 184, 1171
Fnu4HI GCNGC 6 cut(s) 397, 466, 1064, 1361, 1545, 1548
FokI GGATG 2 cut(s) 695, 893
FriOI GRGCYC 1 cut(s) 837
Fsp4HI GCNGC 6 cut(s) 397, 466, 1064, 1361, 1545, 1548
FspBI CTAG 2 cut(s) 339, 1121
FspI TGCGCA 1 cut(s) 826
GlaI GCGC 1 cut(s) 826
GluI GCNGC 6 cut(s) 397, 466, 1064, 1361, 1545, 1548
GsuI CTGGAG 1 cut(s) 418
HaeIII GGCC 4 cut(s) 303, 575, 581, 968
HapII CCGG 1 cut(s) 1405
HgaI GACGC 2 cut(s) 1693, 2087
HhaI GCGC 1 cut(s) 827
Hin1I GRCGYC 2 cut(s) 1685, 2079
Hin6I GCGC 1 cut(s) 825
HinP1I GCGC 1 cut(s) 825
HincII GTYRAC 1 cut(s) 682
HindII GTYRAC 1 cut(s) 682
HinfI GANTC 5 cut(s) 359, 484, 509, 1209, 1444
HpaII CCGG 1 cut(s) 1405
Hpy166II GTNNAC 5 cut(s) 221, 682, 752, 1201, 1929
Hpy188I TCNGA 7 cut(s) 345, 1171, 1427, 1449, 1768, 1803, 1845
Hpy188III TCNNGA 9 cut(s) 25, 112, 259, 293, 334, 743, 1175, 1611, 1697
Hpy8I GTNNAC 5 cut(s) 221, 682, 752, 1201, 1929
Hpy99I CGWCG 1 cut(s) 1687
HpyAV CCTTC 4 cut(s) 200, 206, 1703, 1738
HpyCH4III ACNGT 4 cut(s) 287, 749, 1342, 1417
HpyCH4IV ACGT 2 cut(s) 514, 1482
HpyCH4V TGCA 8 cut(s) 275, 444, 468, 503, 656, 996, 1324, 2024
HpyF10VI GCNNNNNNNGC 4 cut(s) 309, 587, 1463, 1553
HpyF3I CTNAG 3 cut(s) 831, 1302, 1659
HpySE526I ACGT 2 cut(s) 514, 1482
Hsp92I GRCGYC 2 cut(s) 1685, 2079
HspAI GCGC 1 cut(s) 825
KpnI GGTACC 1 cut(s) 1354
Ksp22I TGATCA 2 cut(s) 184, 1171
Kzo9I GATC 8 cut(s) 41, 184, 225, 369, 739, 874, 1171, 1242
LmnI GCTCC 3 cut(s) 437, 1516, 1867
Lsp1109I GCAGC 5 cut(s) 408, 452, 1075, 1372, 1531
LweI GCATC 5 cut(s) 643, 1296, 1669, 1908, 1968
MaeI CTAG 2 cut(s) 339, 1121
MaeII ACGT 2 cut(s) 514, 1482
MaeIII GTNAC 4 cut(s) 25, 491, 1758, 1787
MalI GATC 8 cut(s) 43, 186, 227, 371, 741, 876, 1173, 1244
MboI GATC 8 cut(s) 41, 184, 225, 369, 739, 874, 1171, 1242
MboII GAAGA 8 cut(s) 66, 533, 614, 833, 1654, 1793, 1839, 1927
MflI RGATCY 1 cut(s) 1242
MhlI GDGCHC 3 cut(s) 708, 837, 1521
MluCI AATT 6 cut(s) 280, 729, 758, 771, 1011, 1984
MlyI GAGTC 1 cut(s) 493
MmeI TCCRAC 3 cut(s) 157, 699, 1970
Mph1103I ATGCAT 1 cut(s) 505
MroXI GAANNNNTTC 1 cut(s) 36
MslI CAYNNNNRTG 5 cut(s) 170, 1115, 1391, 1588, 1936
MspA1I CMGCKG 2 cut(s) 399, 1363
MspI CCGG 1 cut(s) 1405
MspR9I CCNGG 5 cut(s) 489, 578, 1157, 1528, 1956
MvaI CCWGG 5 cut(s) 489, 578, 1157, 1528, 1956
MvnI CGCG 1 cut(s) 1319
MwoI GCNNNNNNNGC 4 cut(s) 309, 587, 1463, 1553
NcoI CCATGG 2 cut(s) 1310, 1386
NdeI CATATG 1 cut(s) 871
NdeII GATC 8 cut(s) 41, 184, 225, 369, 739, 874, 1171, 1242
NlaIV GGNNCC 5 cut(s) 20, 1244, 1352, 1857, 1877
NmuCI GTSAC 2 cut(s) 25, 491
NsbI TGCGCA 1 cut(s) 826
NsiI ATGCAT 1 cut(s) 505
NspI RCATGY 1 cut(s) 566
OliI CACNNNNGTG 1 cut(s) 1588
PagI TCATGA 2 cut(s) 111, 258
PciI ACATGT 1 cut(s) 562
PcsI WCGNNNNNNNCGW 2 cut(s) 1209, 1591
PdmI GAANNNNTTC 1 cut(s) 36
PfeI GAWTC 4 cut(s) 359, 509, 1209, 1444
Pfl23II CGTACG 1 cut(s) 515
PflMI CCANNNNNTGG 1 cut(s) 1033
PkrI GCNGC 6 cut(s) 398, 467, 1065, 1362, 1546, 1549
PleI GAGTC 1 cut(s) 492
PpsI GAGTC 1 cut(s) 492
Ppu21I YACGTR 1 cut(s) 515
PscI ACATGT 1 cut(s) 562
PshAI GACNNNNGTC 1 cut(s) 1688
PsiI TTATAA 1 cut(s) 533
Psp124BI GAGCTC 1 cut(s) 837
Psp1406I AACGTT 1 cut(s) 1482
Psp6I CCWGG 5 cut(s) 487, 576, 1155, 1526, 1954
PspGI CCWGG 5 cut(s) 487, 576, 1155, 1526, 1954
PspLI CGTACG 1 cut(s) 515
PspN4I GGNNCC 5 cut(s) 20, 1244, 1352, 1857, 1877
PspPI GGNCC 4 cut(s) 18, 301, 574, 966
PstNI CAGNNNCTG 1 cut(s) 441
PsuI RGATCY 1 cut(s) 1242
RsaI GTAC 7 cut(s) 517, 561, 1202, 1316, 1352, 1928, 1967
RsaNI GTAC 7 cut(s) 516, 560, 1201, 1315, 1351, 1927, 1966
RseI CAYNNNNRTG 5 cut(s) 170, 1115, 1391, 1588, 1936
SacI GAGCTC 1 cut(s) 837
SatI GCNGC 6 cut(s) 397, 466, 1064, 1361, 1545, 1548
Sau3AI GATC 8 cut(s) 41, 184, 225, 369, 739, 874, 1171, 1242
Sau96I GGNCC 4 cut(s) 18, 301, 574, 966
SchI GAGTC 1 cut(s) 493
ScrFI CCNGG 5 cut(s) 489, 578, 1157, 1528, 1956
SduI GDGCHC 3 cut(s) 708, 837, 1521
SfaNI GCATC 5 cut(s) 643, 1296, 1669, 1908, 1968
SinI GGWCC 1 cut(s) 18
SmiMI CAYNNNNRTG 5 cut(s) 170, 1115, 1391, 1588, 1936
SmlI CTYRAG 3 cut(s) 713, 1355, 1898
SmoI CTYRAG 3 cut(s) 713, 1355, 1898
SpeI ACTAGT 1 cut(s) 338
Sse9I AATT 6 cut(s) 280, 729, 758, 771, 1011, 1984
SsiI CCGC 6 cut(s) 399, 1061, 1319, 1363, 1547, 2003
SspI AATATT 2 cut(s) 382, 1328
SspMI CTAG 2 cut(s) 339, 1121
SstI GAGCTC 1 cut(s) 837
StyD4I CCNGG 5 cut(s) 487, 576, 1155, 1526, 1954
StyI CCWWGG 2 cut(s) 1310, 1386
TaaI ACNGT 4 cut(s) 287, 749, 1342, 1417
TaiI ACGT 2 cut(s) 517, 1485
TaqII GACCGA 2 cut(s) 66, 131
TasI AATT 6 cut(s) 280, 729, 758, 771, 1011, 1984
TatI WGTACW 2 cut(s) 559, 1965
TauI GCSGC 1 cut(s) 1550
TfiI GAWTC 4 cut(s) 359, 509, 1209, 1444
TscAI CASTG 6 cut(s) 754, 856, 1003, 1084, 1877, 2026
TseFI GTSAC 2 cut(s) 25, 491
TseI GCWGC 5 cut(s) 396, 465, 1063, 1360, 1544
Tsp45I GTSAC 2 cut(s) 25, 491
TspDTI ATGAA 8 cut(s) 275, 311, 615, 895, 1173, 1415, 1785, 1985
TspGWI ACGGA 3 cut(s) 238, 533, 1475
TspRI CASTG 6 cut(s) 754, 856, 1003, 1084, 1877, 2026
Van91I CCANNNNNTGG 1 cut(s) 1033
VpaK11BI GGWCC 1 cut(s) 18
XceI RCATGY 1 cut(s) 566
XcmI CCANNNNNNNNNTGG 1 cut(s) 2026
XmnI GAANNNNTTC 1 cut(s) 36
XspI CTAG 2 cut(s) 339, 1121
Zsp2I ATGCAT 1 cut(s) 505
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.