Rh4DG023900

Sieve element occlusion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000511
Physical Location & Seq
Forward (+)
3962100 .. 3965417
3318 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG023900.1

Sequence Viewer

Length: 1956 bp
ATGTCTGACAAGAAGATCTTGGAAGAGATTTACACAACTCATGTCCATACTGATGAATCCTTCGATGACGATTCTCTCTTCGTCATTGTTGAGAACATCCTCAAGCGTGCAATCCAAAATGTTGATAAAATAGTACAGGGTACTCAAGTGCATGTGGACAACATAGAGGAGAAAACTCCCAAAGCCAGTTTCAGCGTACCATTGTGCACGCTAAAGGGAATCTCCTGCGAGATGCAATGCAAGCCTCCTGGTGACGAACTCGCTCACAACACAACAGTGGCAATACTCAAAAAGCTCTCAAAGTATTCATGGGAAGCAAAGGCATCACTGACTCTAGCAGCTTTTGCTATGGAATATGGTGAATTCTGGCTCCTGGCGCAGCTTCGCGAATCAGACCACCTTGCCAAATCAATTGCAATCCTCAAACGAGTCCCTGTTCTCCTCAAGCCGTCGGAGCTGCACAAGCGCAGGCAATCAATTCTTGAGCTTAACAATCTGATCAAGGCCACAATGCAAGTGACCGAATGCATTGACCAGTTCAATAAGTATTCCACTTATGACCCAAAAGATGTCCCCGATTTGTCGATAGCATTGGATCATATTCCGGTTGATGTCTATTGGCTTATCATCACTGTTGTTGCTTGTGCTACTAAGATCACTATACTCACTAGTGATGAGGACAAGGAGTATGATCTAGCCCCCTATTCTCAAAAAATCCACAATGTCCTTAACAAGCTAACGAGTTTGCTGAGAGGTTGCAGAAAACAAATTGAGGAGGCAGAGAATTACAGAAGGATCACGAAATTGTTCCGAACCCCTACTGAAATTATGGAGGTTTTCAAGGGATTGATATTTACAAAGGATAATGTCCAGCCACTCATCGATGGTTCAACTAAGCAAACGGTTAATATCGACATCCTAAGGAGAAAGAATGTGTTATTGTTCATTTCCACCCTAGACGTCTCGGACGATGACATTTCGATTCTGAAACCGATCCATGAGTTCACAAAGAGAGACAATCAGTATAAGATTGTCTGGATTCCAATTGTGGAGCAATGGACTGATGACCTGAGAAAAAAGTTTGATATCCTGAAGAACAAGATGCTATGGTTTACGGTGCAGTACTCTGGACCTATTGCAGGCATCAAGTTCATCAAGGAGGAGTGGAATTTCAAGGGTAAGCCTATAGTGGTGGTGATGAACCCACAAGGAAAGGTCGAACACTCAAACGCTCTCCACATGATTCGAGTATGGGGAGCCAAGGCCTTCCCTTTCACAAAGGCAACAGAAGAAGAACTGTCACATTCTCATGCAGGAAAATGGGTTGGCTCCGTTGTTGAAGGAACTCACCCATCTGTGCACACTTGGATCAAAGAGGATAGGTACATCTTCTTCTATGGCGGTAAAGATAACGACTGGATTCAGCAATTCACAAAGAAAGCGACTGCCCTTGCAAATGATCCAATCTTCAAGGAAGCAAAGATTCACCTAGAGTTGGTCTGCGTCGGAAAAGGCAGCAAGGGAGAAGATGACCTTGCGGTTCTAGGCCGCTTCTGGACCAGTGTAGAGAGCCTGTTCTTCACCAAAGGCCACAAGCAGATGGACTCTGTCAATCAAGAAACCCAGAAGCTTCTCTCTTATAAAAACGAAAGTGGATGGGCGGTGCTTAGCAAAGGGTCGACTGTGTTGGTCACAGGTCATGGTGTCTCGATGTTGAAGGTGGTTGAGGACTTTGAGAAATGGAAGGACCATGTCAAGGATAATGGGTTTGAATTTTGTTTCAAGTTCTATCATGAGAAGATTAGTCAGGCTAGTCGACCTTGCTGCTGTCTGGACATTCCGGGGTCTAATGGAAAGGTTCCGGAGTCGATGAGGTGCCCTGATTGCCACCGTAATATGGAGACTTTTGTCAGTTACAAGTGCTGCCACATTGATGGTCCTACTGAACATCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SEO_C SEO_N

Protein Analysis

651

Amino Acids

74.5

Weight (kDa)

6.93

Isoelectric Point (pI)

35.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SEO_N PF14576 2 - 273 1.5e-85 Sieve element occlusion N-terminus
SEO_C PF14577 445 - 643 1.9e-45 Sieve element occlusion C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000511)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g02370 FvH4_4g02370 FvH4_4g02370 FvH4_4g02430 FvH4_4g02430 FvH4_4g02460 FvH4_4g02460 FvH4_4g02460 FvH4_4g02480 FvH4_4g02480 FvH4_4g02480 FvH4_4g02480 FvH4_6g05920
malus_domestica MD00G1111000.v1.1 MD08G1225800.v1.1 MD13G1205700.v1.1 MD13G1205800.v1.1 MD13G1205900.v1.1 MD13G1206000.v1.1 MD16G1207500.v1.1 MD16G1207600.v1.1
prunus_persica Prupe.1G028400_v2.0.a1 Prupe.1G028500_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028900_v2.0.a1 Prupe.1G029000_v2.0.a1 Prupe.1G029100_v2.0.a1
pyrus_communis pycom08g19680 pycom08g19730 pycom16g17460 pycom16g17500
rosa_chinensis RchiOBHm_Chr4g0389751 RchiOBHm_Chr4g0389771 RchiOBHm_Chr4g0389791 RchiOBHm_Chr4g0389861 RchiOBHm_Chr4g0389881 RchiOBHm_Chr4g0389901 RchiOBHm_Chr4g0412411
rosa_laevigata RLG00000008288 RLG00000009901 RLG00000009945 RLG00000009954 RLG00000009956 RLG00000009957 RLG00000009958 RLG00000019246
rosa_multiflora Rmu_co8275125.1_g000001 Rmu_sc0002083.1_g000003 Rmu_sc0002083.1_g000025 Rmu_sc0002604.1_g000016 Rmu_sc0004336.1_g000031 Rmu_sc0010423.1_g000003
rosa_roxburghii Rroxscaffold_5G00335790 Rroxscaffold_5G00335850 Rroxscaffold_5G00335970 Rroxscaffold_5G00335990
rosa_rugosa Rorug03G0325100 Rorug03G0325300 Rorug03G0325500.1 Rorug03G0326800 Rorug03G0327000 Rorug04G0104400
rosa_samantha Rh4AG029900 Rh4AG030000 Rh4AG030100 Rh4AG030200 Rh4AG030600 Rh4AG172900 Rh4BG023200 Rh4BG023900 Rh4BG024000 Rh4BG024100 Rh4BG172200 Rh4DG023300 Rh4DG023800 Rh4DG023900 Rh4DG024000 Rh4DG151600
rosa_wichuraiana Rw1G002330 Rw1G002340 Rw4G002260 Rw4G002270 Rw4G002280 Rw4G002300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1641
AatII GACGTC 1 cut(s) 963
AccB1I GGYRCC 1 cut(s) 1875
AccI GTMKAC 2 cut(s) 1679, 1816
AccII CGCG 1 cut(s) 387
AccIII TCCGGA 1 cut(s) 1861
AciI CCGC 4 cut(s) 1401, 1538, 1549, 1661
AclWI GGATC 5 cut(s) 603, 803, 988, 1376, 1454
AcsI RAATTY 3 cut(s) 362, 1168, 1772
AcuI CTGAAG 1 cut(s) 1112
AcyI GRCGYC 1 cut(s) 960
AfaI GTAC 5 cut(s) 135, 142, 198, 1124, 1385
AfiI CCNNNNNNNGG 4 cut(s) 1139, 1495, 1756, 1897
AgsI TTSAA 9 cut(s) 541, 841, 891, 1174, 1340, 1471, 1717, 1772, 1783
AhlI ACTAGT 1 cut(s) 668
AjnI CCWGG 2 cut(s) 247, 372
AjuI GAANNNNNNNTTGG 2 cut(s) 173, 205
AleI CACNNNNGTG 1 cut(s) 275
AluBI AGCT 7 cut(s) 295, 341, 382, 457, 487, 736, 1630
AluI AGCT 7 cut(s) 295, 341, 382, 457, 487, 736, 1630
Alw21I GWGCWC 2 cut(s) 209, 1362
Alw26I GTCTC 4 cut(s) 967, 1008, 1711, 1895
Alw44I GTGCAC 2 cut(s) 205, 1358
AlwI GGATC 5 cut(s) 603, 803, 988, 1376, 1454
Aor13HI TCCGGA 1 cut(s) 1861
AoxI GGCC 4 cut(s) 504, 1263, 1546, 1588
ApaLI GTGCAC 2 cut(s) 205, 1358
ApeKI GCWGC 6 cut(s) 338, 379, 457, 1515, 1824, 1923
ApoI RAATTY 3 cut(s) 362, 1168, 1772
Asp700I GAANNNNTTC 1 cut(s) 806
AspLEI GCGC 2 cut(s) 379, 468
AspS9I GGNCC 4 cut(s) 1130, 1557, 1747, 1937
AsuC2I CCSGG 1 cut(s) 1842
AsuHPI GGTGA 6 cut(s) 263, 371, 1207, 1340, 1478, 1573
AvaII GGWCC 4 cut(s) 1130, 1557, 1747, 1937
AxyI CCTNAGG 1 cut(s) 920
BaeGI GKGCMC 3 cut(s) 209, 1362, 1880
BaeI ACNNNNGTAYC 2 cut(s) 132, 165
BanI GGYRCC 1 cut(s) 1875
BarI GAAGNNNNNNTAC 2 cut(s) 1376, 1408
Bbv12I GWGCWC 2 cut(s) 209, 1362
BbvI GCAGC 6 cut(s) 350, 391, 444, 1527, 1811, 1910
BccI CCATC 5 cut(s) 878, 1360, 1594, 1650, 1928
BceAI ACGGC 1 cut(s) 433
BcgI CGANNNNNNTGC 2 cut(s) 1806, 1840
BciT130I CCWGG 2 cut(s) 249, 374
BclI TGATCA 1 cut(s) 498
BcnI CCSGG 1 cut(s) 1842
BcoDI GTCTC 4 cut(s) 967, 1008, 1711, 1895
BcuI ACTAGT 1 cut(s) 668
BfaI CTAG 7 cut(s) 335, 669, 695, 956, 1490, 1544, 1812
BfmI CTRYAG 1 cut(s) 1185
BglII AGATCT 1 cut(s) 15
BisI GCNGC 7 cut(s) 339, 380, 458, 1516, 1549, 1825, 1924
BlpI GCTNAGC 1 cut(s) 1667
BlsI GCNGC 7 cut(s) 340, 381, 459, 1517, 1550, 1826, 1925
BmcAI AGTACT 1 cut(s) 1124
Bme1390I CCNGG 3 cut(s) 249, 374, 1842
Bme18I GGWCC 4 cut(s) 1130, 1557, 1747, 1937
BmgT120I GGNCC 4 cut(s) 1130, 1557, 1747, 1937
BmiI GGNNCC 5 cut(s) 371, 1258, 1330, 1860, 1877
BmrFI CCNGG 3 cut(s) 249, 374, 1842
BmsI GCATC 4 cut(s) 222, 332, 1092, 1152
BoxI GACNNNNGTC 1 cut(s) 1907
Bpu1102I GCTNAGC 1 cut(s) 1667
BpuEI CTTGAG 4 cut(s) 86, 129, 428, 503
BpuMI CCSGG 1 cut(s) 1842
Bsa29I ATCGAT 1 cut(s) 882
BsaHI GRCGYC 1 cut(s) 960
BsaJI CCNNGG 2 cut(s) 1260, 1841
BsaWI WCCGGW 2 cut(s) 604, 1861
Bsc4I CCNNNNNNNGG 4 cut(s) 1139, 1495, 1756, 1897
Bse1I ACTGG 4 cut(s) 186, 535, 1421, 1560
Bse21I CCTNAGG 1 cut(s) 920
Bse3DI GCAATG 2 cut(s) 242, 1061
BseAI TCCGGA 1 cut(s) 1861
BseBI CCWGG 2 cut(s) 249, 374
BseCI ATCGAT 1 cut(s) 882
BseDI CCNNGG 2 cut(s) 1260, 1841
BseGI GGATG 3 cut(s) 96, 915, 1661
BseLI CCNNNNNNNGG 4 cut(s) 1139, 1495, 1756, 1897
BseMI GCAATG 2 cut(s) 242, 1061
BseMII CTCAG 2 cut(s) 740, 1061
BseNI ACTGG 4 cut(s) 186, 535, 1421, 1560
BseRI GAGGAG 4 cut(s) 182, 431, 788, 1175
BseSI GKGCMC 3 cut(s) 209, 1362, 1880
BseXI GCAGC 6 cut(s) 350, 391, 444, 1527, 1811, 1910
BsgI GTGCAG 2 cut(s) 443, 1139
Bsh1236I CGCG 1 cut(s) 387
BshFI GGCC 4 cut(s) 506, 1265, 1548, 1590
BshNI GGYRCC 1 cut(s) 1875
BshVI ATCGAT 1 cut(s) 882
BsiHKAI GWGCWC 2 cut(s) 209, 1362
BsiSI CCGG 3 cut(s) 605, 1841, 1862
BslFI GGGAC 2 cut(s) 416, 557
BslI CCNNNNNNNGG 4 cut(s) 1139, 1495, 1756, 1897
BsmAI GTCTC 4 cut(s) 967, 1008, 1711, 1895
BsmBI CGTCTC 1 cut(s) 967
BsmFI GGGAC 2 cut(s) 416, 557
BsmI GAATGC 1 cut(s) 530
BsnI GGCC 4 cut(s) 506, 1265, 1548, 1590
Bsp1286I GDGCHC 3 cut(s) 209, 1362, 1880
Bsp13I TCCGGA 1 cut(s) 1861
Bsp143I GATC 9 cut(s) 15, 498, 595, 654, 691, 795, 993, 1368, 1459
Bsp1720I GCTNAGC 1 cut(s) 1667
Bsp68I TCGCGA 1 cut(s) 387
BspACI CCGC 4 cut(s) 1401, 1538, 1549, 1661
BspANI GGCC 4 cut(s) 506, 1265, 1548, 1590
BspCNI CTCAG 2 cut(s) 741, 1062
BspDI ATCGAT 1 cut(s) 882
BspEI TCCGGA 1 cut(s) 1861
BspFNI CGCG 1 cut(s) 387
BspHI TCATGA 1 cut(s) 1792
BspLI GGNNCC 5 cut(s) 371, 1258, 1330, 1860, 1877
BspPI GGATC 5 cut(s) 603, 803, 988, 1376, 1454
BspT107I GGYRCC 1 cut(s) 1875
BsrDI GCAATG 2 cut(s) 242, 1061
BsrI ACTGG 4 cut(s) 186, 535, 1421, 1560
BssECI CCNNGG 2 cut(s) 1260, 1841
BssMI GATC 9 cut(s) 15, 498, 595, 654, 691, 795, 993, 1368, 1459
BssNI GRCGYC 1 cut(s) 960
BssT1I CCWWGG 1 cut(s) 1260
Bst2UI CCWGG 2 cut(s) 249, 374
Bst4CI ACNGT 7 cut(s) 277, 634, 904, 1117, 1299, 1684, 1892
Bst6I CTCTTC 2 cut(s) 18, 83
BstACI GRCGYC 1 cut(s) 960
BstAPI GCANNNNNTGC 1 cut(s) 344
BstC8I GCNNGC 5 cut(s) 108, 209, 242, 470, 1141
BstDEI CTNAG 6 cut(s) 651, 749, 894, 920, 1070, 1667
BstENI CCTNNNNNAGG 1 cut(s) 1137
BstF5I GGATG 3 cut(s) 96, 915, 1661
BstFNI CGCG 1 cut(s) 387
BstHHI GCGC 2 cut(s) 379, 468
BstKTI GATC 9 cut(s) 18, 501, 598, 657, 694, 798, 996, 1371, 1462
BstMAI GTCTC 4 cut(s) 967, 1008, 1711, 1895
BstMBI GATC 9 cut(s) 15, 498, 595, 654, 691, 795, 993, 1368, 1459
BstMWI GCNNNNNNNGC 6 cut(s) 241, 344, 376, 454, 463, 1884
BstNI CCWGG 2 cut(s) 249, 374
BstNSI RCATGY 1 cut(s) 155
BstPAI GACNNNNGTC 1 cut(s) 1907
BstSCI CCNGG 3 cut(s) 247, 372, 1840
BstSFI CTRYAG 1 cut(s) 1185
BstSLI GKGCMC 3 cut(s) 209, 1362, 1880
BstUI CGCG 1 cut(s) 387
BstV1I GCAGC 6 cut(s) 350, 391, 444, 1527, 1811, 1910
BstX2I RGATCY 1 cut(s) 15
BstXI CCANNNNNNTGG 1 cut(s) 1934
BstYI RGATCY 1 cut(s) 15
Bsu15I ATCGAT 1 cut(s) 882
Bsu36I CCTNAGG 1 cut(s) 920
BsuRI GGCC 4 cut(s) 506, 1265, 1548, 1590
BsuTUI ATCGAT 1 cut(s) 882
BtsCI GGATG 3 cut(s) 96, 915, 1661
BtsIMutI CAGTG 4 cut(s) 282, 326, 630, 1567
BtuMI TCGCGA 1 cut(s) 387
Cac8I GCNNGC 5 cut(s) 108, 209, 242, 470, 1141
CciI TCATGA 1 cut(s) 1792
CfoI GCGC 2 cut(s) 379, 468
Cfr13I GGNCC 4 cut(s) 1130, 1557, 1747, 1937
ClaI ATCGAT 1 cut(s) 882
CseI GACGC 1 cut(s) 1492
Csp6I GTAC 5 cut(s) 134, 141, 197, 1123, 1384
CviAII CATG 9 cut(s) 41, 152, 309, 998, 1240, 1310, 1700, 1751, 1793
CviQI GTAC 5 cut(s) 134, 141, 197, 1123, 1384
DdeI CTNAG 6 cut(s) 651, 749, 894, 920, 1070, 1667
DpnI GATC 9 cut(s) 17, 500, 597, 656, 693, 797, 995, 1370, 1461
DpnII GATC 9 cut(s) 15, 498, 595, 654, 691, 795, 993, 1368, 1459
Eam1104I CTCTTC 2 cut(s) 18, 83
EarI CTCTTC 2 cut(s) 18, 83
Eco130I CCWWGG 1 cut(s) 1260
Eco147I AGGCCT 1 cut(s) 1265
Eco32I GATATC 1 cut(s) 1087
Eco47I GGWCC 4 cut(s) 1130, 1557, 1747, 1937
Eco57I CTGAAG 1 cut(s) 1112
Eco81I CCTNAGG 1 cut(s) 920
EcoNI CCTNNNNNAGG 1 cut(s) 1137
EcoRI GAATTC 1 cut(s) 362
EcoRII CCWGG 2 cut(s) 247, 372
EcoRV GATATC 1 cut(s) 1087
EcoT14I CCWWGG 1 cut(s) 1260
EcoT22I ATGCAT 1 cut(s) 530
ErhI CCWWGG 1 cut(s) 1260
Esp3I CGTCTC 1 cut(s) 967
FaeI CATG 9 cut(s) 44, 155, 312, 1001, 1243, 1313, 1703, 1754, 1796
FalI AAGNNNNNCTT 3 cut(s) 34, 1518, 1550
FaqI GGGAC 2 cut(s) 416, 557
FatI CATG 9 cut(s) 40, 151, 308, 997, 1239, 1309, 1699, 1750, 1792
FbaI TGATCA 1 cut(s) 498
FblI GTMKAC 2 cut(s) 1679, 1816
Fnu4HI GCNGC 7 cut(s) 339, 380, 458, 1516, 1549, 1825, 1924
FokI GGATG 3 cut(s) 83, 902, 1668
Fsp4HI GCNGC 7 cut(s) 339, 380, 458, 1516, 1549, 1825, 1924
FspBI CTAG 7 cut(s) 335, 669, 695, 956, 1490, 1544, 1812
GlaI GCGC 2 cut(s) 378, 467
GluI GCNGC 7 cut(s) 339, 380, 458, 1516, 1549, 1825, 1924
HaeIII GGCC 4 cut(s) 506, 1265, 1548, 1590
HapII CCGG 3 cut(s) 605, 1841, 1862
HgaI GACGC 1 cut(s) 1492
HhaI GCGC 2 cut(s) 379, 468
Hin1I GRCGYC 1 cut(s) 960
Hin1II CATG 9 cut(s) 44, 155, 312, 1001, 1243, 1313, 1703, 1754, 1796
Hin6I GCGC 2 cut(s) 377, 466
HinP1I GCGC 2 cut(s) 377, 466
HincII GTYRAC 2 cut(s) 1680, 1817
HindII GTYRAC 2 cut(s) 1680, 1817
HindIII AAGCTT 1 cut(s) 1628
HpaII CCGG 3 cut(s) 605, 1841, 1862
HphI GGTGA 6 cut(s) 263, 371, 1207, 1340, 1478, 1573
Hpy166II GTNNAC 7 cut(s) 157, 207, 1005, 1113, 1360, 1680, 1817
Hpy188I TCNGA 8 cut(s) 7, 394, 454, 498, 812, 967, 987, 1508
Hpy8I GTNNAC 7 cut(s) 157, 207, 1005, 1113, 1360, 1680, 1817
Hpy99I CGWCG 2 cut(s) 454, 1508
HpyAV CCTTC 6 cut(s) 70, 786, 1276, 1334, 1711, 1738
HpyCH4III ACNGT 7 cut(s) 277, 634, 904, 1117, 1299, 1684, 1892
HpyCH4IV ACGT 1 cut(s) 960
HpyF10VI GCNNNNNNNGC 6 cut(s) 241, 344, 376, 454, 463, 1884
HpyF3I CTNAG 6 cut(s) 651, 749, 894, 920, 1070, 1667
HpySE526I ACGT 1 cut(s) 960
Hsp92I GRCGYC 1 cut(s) 960
Hsp92II CATG 9 cut(s) 44, 155, 312, 1001, 1243, 1313, 1703, 1754, 1796
HspAI GCGC 2 cut(s) 377, 466
Kpn2I TCCGGA 1 cut(s) 1861
Ksp22I TGATCA 1 cut(s) 498
Kzo9I GATC 9 cut(s) 15, 498, 595, 654, 691, 795, 993, 1368, 1459
LmnI GCTCC 5 cut(s) 375, 454, 1051, 1256, 1334
Lsp1109I GCAGC 6 cut(s) 350, 391, 444, 1527, 1811, 1910
LweI GCATC 4 cut(s) 222, 332, 1092, 1152
MaeI CTAG 7 cut(s) 335, 669, 695, 956, 1490, 1544, 1812
MaeII ACGT 1 cut(s) 960
MaeIII GTNAC 5 cut(s) 251, 517, 1299, 1690, 1913
MalI GATC 9 cut(s) 17, 500, 597, 656, 693, 797, 995, 1370, 1461
MboI GATC 9 cut(s) 15, 498, 595, 654, 691, 795, 993, 1368, 1459
MfeI CAATTG 2 cut(s) 411, 1044
MflI RGATCY 1 cut(s) 15
MhlI GDGCHC 3 cut(s) 209, 1362, 1880
MlyI GAGTC 4 cut(s) 325, 438, 1598, 1874
MmeI TCCRAC 2 cut(s) 432, 1486
Mph1103I ATGCAT 1 cut(s) 530
MroI TCCGGA 1 cut(s) 1861
MroXI GAANNNNTTC 1 cut(s) 806
MseI TTAA 4 cut(s) 489, 729, 906, 1954
MslI CAYNNNNRTG 4 cut(s) 51, 275, 1308, 1932
MspI CCGG 3 cut(s) 605, 1841, 1862
MspR9I CCNGG 3 cut(s) 249, 374, 1842
MunI CAATTG 2 cut(s) 411, 1044
Mva1269I GAATGC 1 cut(s) 530
MvaI CCWGG 2 cut(s) 249, 374
MvnI CGCG 1 cut(s) 387
MwoI GCNNNNNNNGC 6 cut(s) 241, 344, 376, 454, 463, 1884
NciI CCSGG 1 cut(s) 1842
NdeII GATC 9 cut(s) 15, 498, 595, 654, 691, 795, 993, 1368, 1459
NlaIII CATG 9 cut(s) 44, 155, 312, 1001, 1243, 1313, 1703, 1754, 1796
NlaIV GGNNCC 5 cut(s) 371, 1258, 1330, 1860, 1877
NmuCI GTSAC 4 cut(s) 251, 517, 1299, 1690
NruI TCGCGA 1 cut(s) 387
NsiI ATGCAT 1 cut(s) 530
NspI RCATGY 1 cut(s) 155
OliI CACNNNNGTG 1 cut(s) 275
PagI TCATGA 1 cut(s) 1792
PceI AGGCCT 1 cut(s) 1265
PcsI WCGNNNNNNNCGW 1 cut(s) 966
PctI GAATGC 1 cut(s) 530
PdmI GAANNNNTTC 1 cut(s) 806
PfeI GAWTC 9 cut(s) 56, 71, 219, 389, 982, 1039, 1243, 1420, 1483
PflFI GACNNNGTC 2 cut(s) 1607, 1751
PkrI GCNGC 7 cut(s) 340, 381, 459, 1517, 1550, 1826, 1925
PleI GAGTC 4 cut(s) 325, 437, 1598, 1873
PpsI GAGTC 4 cut(s) 325, 437, 1598, 1873
PshAI GACNNNNGTC 1 cut(s) 1907
PsiI TTATAA 1 cut(s) 1641
Psp6I CCWGG 2 cut(s) 247, 372
PspGI CCWGG 2 cut(s) 247, 372
PspN4I GGNNCC 5 cut(s) 371, 1258, 1330, 1860, 1877
PspPI GGNCC 4 cut(s) 1130, 1557, 1747, 1937
PsuI RGATCY 1 cut(s) 15
PsyI GACNNNGTC 2 cut(s) 1607, 1751
RruI TCGCGA 1 cut(s) 387
RsaI GTAC 5 cut(s) 135, 142, 198, 1124, 1385
RsaNI GTAC 5 cut(s) 134, 141, 197, 1123, 1384
RseI CAYNNNNRTG 4 cut(s) 51, 275, 1308, 1932
SalI GTCGAC 2 cut(s) 1678, 1815
SaqAI TTAA 4 cut(s) 489, 729, 906, 1954
SatI GCNGC 7 cut(s) 339, 380, 458, 1516, 1549, 1825, 1924
Sau3AI GATC 9 cut(s) 15, 498, 595, 654, 691, 795, 993, 1368, 1459
Sau96I GGNCC 4 cut(s) 1130, 1557, 1747, 1937
ScaI AGTACT 1 cut(s) 1124
SchI GAGTC 4 cut(s) 325, 438, 1598, 1874
ScrFI CCNGG 3 cut(s) 249, 374, 1842
SduI GDGCHC 3 cut(s) 209, 1362, 1880
SfaNI GCATC 4 cut(s) 222, 332, 1092, 1152
SfcI CTRYAG 1 cut(s) 1185
SinI GGWCC 4 cut(s) 1130, 1557, 1747, 1937
SmiMI CAYNNNNRTG 4 cut(s) 51, 275, 1308, 1932
SmlI CTYRAG 4 cut(s) 101, 144, 443, 482
SmoI CTYRAG 4 cut(s) 101, 144, 443, 482
SpeI ACTAGT 1 cut(s) 668
SseBI AGGCCT 1 cut(s) 1265
SsiI CCGC 4 cut(s) 1401, 1538, 1549, 1661
SspMI CTAG 7 cut(s) 335, 669, 695, 956, 1490, 1544, 1812
StuI AGGCCT 1 cut(s) 1265
StyD4I CCNGG 3 cut(s) 247, 372, 1840
StyI CCWWGG 1 cut(s) 1260
TaaI ACNGT 7 cut(s) 277, 634, 904, 1117, 1299, 1684, 1892
TaiI ACGT 1 cut(s) 963
TaqII GACCGA 1 cut(s) 536
TatI WGTACW 2 cut(s) 133, 1122
TauI GCSGC 1 cut(s) 1551
TfiI GAWTC 9 cut(s) 56, 71, 219, 389, 982, 1039, 1243, 1420, 1483
Tru1I TTAA 4 cut(s) 489, 729, 906, 1954
Tru9I TTAA 4 cut(s) 489, 729, 906, 1954
TscAI CASTG 4 cut(s) 282, 333, 637, 1567
TseFI GTSAC 4 cut(s) 251, 517, 1299, 1690
TseI GCWGC 6 cut(s) 338, 379, 457, 1515, 1824, 1923
Tsp45I GTSAC 4 cut(s) 251, 517, 1299, 1690
TspDTI ATGAA 5 cut(s) 69, 297, 934, 1141, 1214
TspGWI ACGGA 1 cut(s) 1321
TspRI CASTG 4 cut(s) 282, 333, 637, 1567
Tth111I GACNNNGTC 2 cut(s) 1607, 1751
VneI GTGCAC 2 cut(s) 205, 1358
VpaK11BI GGWCC 4 cut(s) 1130, 1557, 1747, 1937
XagI CCTNNNNNAGG 1 cut(s) 1137
XapI RAATTY 3 cut(s) 362, 1168, 1772
XceI RCATGY 1 cut(s) 155
XmiI GTMKAC 2 cut(s) 1679, 1816
XmnI GAANNNNTTC 1 cut(s) 806
XspI CTAG 7 cut(s) 335, 669, 695, 956, 1490, 1544, 1812
ZraI GACGTC 1 cut(s) 961
ZrmI AGTACT 1 cut(s) 1124
Zsp2I ATGCAT 1 cut(s) 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.