MD08G1225800.v1.1

Sieve element occlusion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000511
Physical Location & Seq
Forward (+)
29260760 .. 29266340
5581 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1225800.v1.1.491

Sequence Viewer

Length: 1986 bp
ATGAGTAAGAGGATGAATGGGGGTGAGGGTGAGGGTGGTGAACGGATCAGCCTTTTCGACAGGTCTCAAAACGAAATCATGAAGCTCATTTTTGCAGTGAATTACCGTGTTCCTGATGAGGGCCATGCCAGCTTTGATGTTCATGCTATTTTCTCGACTAGTCAGAAAATCATTAAGGATTCGGCCAACATCATTCACAATATTAATGAGCAGGGCAGCGGATTTAATGATGTTGAAACACCCAAAGCCAGCTCCAGCTTGTGCACTCTTATTAAGGAGAAATTTTTAAATGTGACGGGAACACGATTCGGTACCTCAAGTATCATAATACGAGTGGTTGGAAATTGGATAAAAAAAAATTTCAACAACTTGTTTTATGACACTCGGTGTACCGACCCATATTCCCGGCACTTTGAAAAATCTCTTCTTATTATTAACTCCTTTGGCTGCAAGATGTCGTGTAAGAGTTCAGGTGATGAAACTTCCTTTGAATCACGTACGGAAGAAATACTTACAAAACTATCGAGTTATCCATGTGAAGTACAGGTAGTATTGGCCCTGGCAGTTTTTGCTTTGGAATATGAAGAATGTTCCAGTCAACTTTCCAAGAAATCGACAACGCTGAAACGCCAAGATGCAGTAGTCGAGCTTAACAATCTGGTCAACCAAGCGTTACAAGTGATTGAGCACATCCTTGAGTTGGAGAAACTAGTTTCTTATGATCGAGACAGTGAACCAAAATTGGCGAGGGTAATTATGGATACCCCATTTAATGCCTACTGGTCAATCATAACTCTTGTAGCTTGCGCAACTGAGCTCTTTTTTCTTACCGGTGATGAGGACATTAAGTCATATGATCTATCCCATTTTTCTCACAAAATGAGCTTCATCCTCAACAAGCTTGAGGGACAGCTTACAATCTGCCAACAAGAAAATGAGGAGGCAAAGGCCAAGGCCGAGGAAAAGTTTTGGGAACAAATACGCACTGCTTCTGGAATTACCGAGGTTTTCCGAAGGCTGGTTCTTTTCAAGCATAAAGGGTATCCGCAGCCTCGCATCAGTGTTGATCCCGCTAACACGGAGGTTTTTAACATTAATGTGCTAGAGTGGGTGTTATTTTACATTTCAAGCCTGGACAATGTTTCTGATCAAGATATTTCATGTCTCAAAGAAGTGTATGAGGGAATCCAAAAGTATAACAATTGTGTGATTGTGTGGATCCCTGTTGTTGAGGACTGGACTGAAGGAGGAGAAGAGCAGTTTAAGGAATGGAGGTCTAAGATGCCATGGTACGCGGTGCAATATTTTTCGCCCCTGGCCAGTGGGTACCTTGAGCAGCGGTGGAACTTCGAAGGTAATCCTATGGTGGTGGTGATGAACTGGAAAGGAGAGGTGGCAAACAGCAACATAATCGACTTGATTCGGACACAAGCAACGGAGGCAATCGCTTTGCTTAACGTTGAAATAAAACCAAGACTTGTGGCCCAAGCCTGCTTTGGAACGAATGCTGAAGCTGTGGAGGTGTTGAAGAGCTTAACTTGTCCTCAGCAAATCACTAATACTGATGCGACGCCTGTCCTTCTTGATGGTTCTAACAAGCAGGTTAATATCGACAATGAGCTGAAGTGGAAAGATCTGTTTTTGTTACTCTCCGACGTAGGCATTGAAGATTGTTACATTTCATATCTCAAACCAGTTTATGAGGGAATAAGAAGATACGATGGGTATCGGATTGTCTGGGTTCCCGTTGTGGAGCAGTGGAACCAAGACAAAGGAAATCAACTTGAGATGTCGAGGTTGAAGATGCCGTGGTACACATTGAAGTGTTTTTCAACCATACCAGGCATCAAGTACATGAAAAAGAAGTGGAATTACAAGGGTAAGCCAGCGGTGGTGGTGATGACCTTTACAGGCATGGTGAAAAATGAAAATGCTTTCCCCTTGATTAAGAAACATGGAATGGACGCCTTTCCTTTCTTTAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SEO_C SEO_N

Protein Analysis

662

Amino Acids

75.67

Weight (kDa)

5.65

Isoelectric Point (pI)

44.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SEO_N PF14576 127 - 325 6.8e-28 Sieve element occlusion N-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000511)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g02370 FvH4_4g02370 FvH4_4g02370 FvH4_4g02430 FvH4_4g02430 FvH4_4g02460 FvH4_4g02460 FvH4_4g02460 FvH4_4g02480 FvH4_4g02480 FvH4_4g02480 FvH4_4g02480 FvH4_6g05920
malus_domestica MD00G1111000.v1.1 MD08G1225800.v1.1 MD13G1205700.v1.1 MD13G1205800.v1.1 MD13G1205900.v1.1 MD13G1206000.v1.1 MD16G1207500.v1.1 MD16G1207600.v1.1
prunus_persica Prupe.1G028400_v2.0.a1 Prupe.1G028500_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028900_v2.0.a1 Prupe.1G029000_v2.0.a1 Prupe.1G029100_v2.0.a1
pyrus_communis pycom08g19680 pycom08g19730 pycom16g17460 pycom16g17500
rosa_chinensis RchiOBHm_Chr4g0389751 RchiOBHm_Chr4g0389771 RchiOBHm_Chr4g0389791 RchiOBHm_Chr4g0389861 RchiOBHm_Chr4g0389881 RchiOBHm_Chr4g0389901 RchiOBHm_Chr4g0412411
rosa_laevigata RLG00000008288 RLG00000009901 RLG00000009945 RLG00000009954 RLG00000009956 RLG00000009957 RLG00000009958 RLG00000019246
rosa_multiflora Rmu_co8275125.1_g000001 Rmu_sc0002083.1_g000003 Rmu_sc0002083.1_g000025 Rmu_sc0002604.1_g000016 Rmu_sc0004336.1_g000031 Rmu_sc0010423.1_g000003
rosa_roxburghii Rroxscaffold_5G00335790 Rroxscaffold_5G00335850 Rroxscaffold_5G00335970 Rroxscaffold_5G00335990
rosa_rugosa Rorug03G0325100 Rorug03G0325300 Rorug03G0325500.1 Rorug03G0326800 Rorug03G0327000 Rorug04G0104400
rosa_samantha Rh4AG029900 Rh4AG030000 Rh4AG030100 Rh4AG030200 Rh4AG030600 Rh4AG172900 Rh4BG023200 Rh4BG023900 Rh4BG024000 Rh4BG024100 Rh4BG172200 Rh4DG023300 Rh4DG023800 Rh4DG023900 Rh4DG024000 Rh4DG151600
rosa_wichuraiana Rw1G002330 Rw1G002340 Rw4G002260 Rw4G002270 Rw4G002280 Rw4G002300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 808
Acc36I ACCTGC 1 cut(s) 1591
Acc65I GGTACC 2 cut(s) 311, 1326
AccB1I GGYRCC 2 cut(s) 311, 1326
AccII CGCG 1 cut(s) 1295
AciI CCGC 6 cut(s) 219, 1046, 1071, 1295, 1339, 1889
AclI AACGTT 1 cut(s) 1458
AclWI GGATC 4 cut(s) 53, 1061, 1213, 1226
AcoI YGGCCR 2 cut(s) 183, 1317
AcsI RAATTY 2 cut(s) 281, 358
AcuI CTGAAG 3 cut(s) 1263, 1530, 1643
AcyI GRCGYC 2 cut(s) 1571, 1965
AdeI CACNNNGTG 1 cut(s) 387
AfaI GTAC 8 cut(s) 313, 391, 499, 543, 1292, 1328, 1814, 1853
AfiI CCNNNNNNNGG 3 cut(s) 119, 700, 1018
AgeI ACCGGT 1 cut(s) 830
AhdI GACNNNNNGTC 1 cut(s) 847
AhlI ACTAGT 2 cut(s) 158, 709
AjnI CCWGG 4 cut(s) 558, 1131, 1314, 1840
Alw21I GWGCWC 3 cut(s) 266, 690, 819
Alw26I GTCTC 3 cut(s) 69, 720, 1169
Alw44I GTGCAC 1 cut(s) 262
AlwI GGATC 4 cut(s) 53, 1061, 1213, 1226
AoxI GGCC 7 cut(s) 121, 183, 555, 948, 954, 1317, 1482
ApaLI GTGCAC 1 cut(s) 262
ApeKI GCWGC 4 cut(s) 216, 447, 1048, 1336
ApoI RAATTY 2 cut(s) 281, 358
AseI ATTAAT 2 cut(s) 204, 1095
AsiGI ACCGGT 1 cut(s) 830
Asp718I GGTACC 2 cut(s) 311, 1326
AspLEI GCGC 1 cut(s) 809
AspS9I GGNCC 3 cut(s) 121, 556, 1483
AsuC2I CCSGG 1 cut(s) 406
AsuHPI GGTGA 8 cut(s) 35, 41, 50, 485, 845, 1384, 1909, 1930
AsuII TTCGAA 1 cut(s) 1350
BaeGI GKGCMC 1 cut(s) 266
BalI TGGCCA 1 cut(s) 1319
BamHI GGATCC 1 cut(s) 1218
BanI GGYRCC 2 cut(s) 311, 1326
BanII GRGCYC 1 cut(s) 819
BarI GAAGNNNNNNTAC 2 cut(s) 1856, 1888
Bbv12I GWGCWC 3 cut(s) 266, 690, 819
BbvCI CCTCAGC 1 cut(s) 1545
BbvI GCAGC 4 cut(s) 228, 434, 1060, 1348
BccI CCATC 2 cut(s) 1580, 1715
BceAI ACGGC 1 cut(s) 1792
BcgI CGANNNNNNTGC 2 cut(s) 1393, 1427
BciT130I CCWGG 4 cut(s) 560, 1133, 1316, 1842
BciVI GTATCC 2 cut(s) 754, 1053
BclI TGATCA 1 cut(s) 1147
BcnI CCSGG 1 cut(s) 406
BcoDI GTCTC 3 cut(s) 69, 720, 1169
BcuI ACTAGT 2 cut(s) 158, 709
BfaI CTAG 3 cut(s) 159, 710, 1103
BfuAI ACCTGC 1 cut(s) 1591
BfuI GTATCC 2 cut(s) 754, 1053
BglII AGATCT 1 cut(s) 1633
BisI GCNGC 4 cut(s) 217, 448, 1049, 1337
BlsI GCNGC 4 cut(s) 218, 449, 1050, 1338
Bme1390I CCNGG 5 cut(s) 406, 560, 1133, 1316, 1842
BmeRI GACNNNNNGTC 1 cut(s) 847
BmgT120I GGNCC 3 cut(s) 121, 556, 1483
BmiI GGNNCC 5 cut(s) 313, 1220, 1328, 1743, 1763
BmrFI CCNGG 5 cut(s) 406, 560, 1133, 1316, 1842
BmsI GCATC 6 cut(s) 625, 1065, 1272, 1555, 1794, 1854
BoxI GACNNNNGTC 1 cut(s) 1574
BpmI CTGGAG 1 cut(s) 238
Bpu10I CCTNAGC 1 cut(s) 1545
Bpu14I TTCGAA 1 cut(s) 1350
BpuEI CTTGAG 5 cut(s) 301, 716, 923, 1352, 1805
BpuMI CCSGG 1 cut(s) 406
BsaAI YACGTR 1 cut(s) 497
BsaBI GATNNNNATC 1 cut(s) 1725
BsaHI GRCGYC 2 cut(s) 1571, 1965
BsaI GGTCTC 1 cut(s) 69
BsaJI CCNNGG 7 cut(s) 558, 951, 957, 1002, 1286, 1314, 1808
BsaWI WCCGGW 1 cut(s) 830
Bsc4I CCNNNNNNNGG 3 cut(s) 119, 700, 1018
Bse118I RCCGGY 1 cut(s) 830
Bse1I ACTGG 6 cut(s) 594, 785, 1241, 1320, 1385, 1694
Bse8I GATNNNNATC 1 cut(s) 1725
BseBI CCWGG 4 cut(s) 560, 1133, 1316, 1842
BseDI CCNNGG 7 cut(s) 558, 951, 957, 1002, 1286, 1314, 1808
BseGI GGATG 3 cut(s) 18, 690, 888
BseJI GATNNNNATC 1 cut(s) 1725
BseLI CCNNNNNNNGG 3 cut(s) 119, 700, 1018
BseMII CTCAG 2 cut(s) 804, 1559
BseNI ACTGG 6 cut(s) 594, 785, 1241, 1320, 1385, 1694
BseRI GAGGAG 2 cut(s) 953, 1263
BseSI GKGCMC 1 cut(s) 266
BseXI GCAGC 4 cut(s) 228, 434, 1060, 1348
Bsh1236I CGCG 1 cut(s) 1295
BshFI GGCC 7 cut(s) 123, 185, 557, 950, 956, 1319, 1484
BshNI GGYRCC 2 cut(s) 311, 1326
BshTI ACCGGT 1 cut(s) 830
BsiHKAI GWGCWC 3 cut(s) 266, 690, 819
BsiSI CCGG 2 cut(s) 406, 831
BsiWI CGTACG 1 cut(s) 497
BslFI GGGAC 1 cut(s) 921
BslI CCNNNNNNNGG 3 cut(s) 119, 700, 1018
BsmAI GTCTC 3 cut(s) 69, 720, 1169
BsmFI GGGAC 1 cut(s) 921
BsmI GAATGC 1 cut(s) 1510
BsnI GGCC 7 cut(s) 123, 185, 557, 950, 956, 1319, 1484
Bso31I GGTCTC 1 cut(s) 69
Bsp119I TTCGAA 1 cut(s) 1350
Bsp1286I GDGCHC 3 cut(s) 266, 690, 819
Bsp143I GATC 7 cut(s) 45, 721, 856, 1066, 1147, 1218, 1633
Bsp19I CCATGG 1 cut(s) 1286
BspACI CCGC 6 cut(s) 219, 1046, 1071, 1295, 1339, 1889
BspANI GGCC 7 cut(s) 123, 185, 557, 950, 956, 1319, 1484
BspCNI CTCAG 2 cut(s) 805, 1558
BspFNI CGCG 1 cut(s) 1295
BspHI TCATGA 1 cut(s) 78
BspLI GGNNCC 5 cut(s) 313, 1220, 1328, 1743, 1763
BspMI ACCTGC 1 cut(s) 1591
BspPI GGATC 4 cut(s) 53, 1061, 1213, 1226
BspQI GCTCTTC 2 cut(s) 1248, 1523
BspT104I TTCGAA 1 cut(s) 1350
BspT107I GGYRCC 2 cut(s) 311, 1326
BspTNI GGTCTC 1 cut(s) 69
BsrFI RCCGGY 1 cut(s) 830
BsrI ACTGG 6 cut(s) 594, 785, 1241, 1320, 1385, 1694
BssAI RCCGGY 1 cut(s) 830
BssECI CCNNGG 7 cut(s) 558, 951, 957, 1002, 1286, 1314, 1808
BssMI GATC 7 cut(s) 45, 721, 856, 1066, 1147, 1218, 1633
BssNI GRCGYC 2 cut(s) 1571, 1965
BssT1I CCWWGG 2 cut(s) 951, 1286
Bst2UI CCWGG 4 cut(s) 560, 1133, 1316, 1842
Bst4CI ACNGT 2 cut(s) 107, 731
Bst6I CTCTTC 3 cut(s) 429, 1248, 1523
BstACI GRCGYC 2 cut(s) 1571, 1965
BstAPI GCANNNNNTGC 1 cut(s) 569
BstBAI YACGTR 1 cut(s) 497
BstBI TTCGAA 1 cut(s) 1350
BstC8I GCNNGC 5 cut(s) 130, 250, 805, 1492, 1887
BstDEI CTNAG 3 cut(s) 813, 1278, 1545
BstDSI CCRYGG 2 cut(s) 1286, 1808
BstF5I GGATG 3 cut(s) 18, 690, 888
BstFNI CGCG 1 cut(s) 1295
BstHHI GCGC 1 cut(s) 809
BstKTI GATC 7 cut(s) 48, 724, 859, 1069, 1150, 1221, 1636
BstMAI GTCTC 3 cut(s) 69, 720, 1169
BstMBI GATC 7 cut(s) 45, 721, 856, 1066, 1147, 1218, 1633
BstMWI GCNNNNNNNGC 3 cut(s) 129, 569, 1439
BstNI CCWGG 4 cut(s) 560, 1133, 1316, 1842
BstPAI GACNNNNGTC 1 cut(s) 1574
BstSCI CCNGG 5 cut(s) 404, 558, 1131, 1314, 1840
BstSLI GKGCMC 1 cut(s) 266
BstUI CGCG 1 cut(s) 1295
BstV1I GCAGC 4 cut(s) 228, 434, 1060, 1348
BstX2I RGATCY 2 cut(s) 1218, 1633
BstYI RGATCY 2 cut(s) 1218, 1633
BsuI GTATCC 2 cut(s) 754, 1053
BsuRI GGCC 7 cut(s) 123, 185, 557, 950, 956, 1319, 1484
BtgI CCRYGG 2 cut(s) 1286, 1808
BtsCI GGATG 3 cut(s) 18, 690, 888
BtsI GCAGTG 3 cut(s) 102, 984, 1763
BtsIMutI CAGTG 6 cut(s) 102, 736, 984, 1066, 1327, 1763
BveI ACCTGC 1 cut(s) 1591
Cac8I GCNNGC 5 cut(s) 130, 250, 805, 1492, 1887
CciI TCATGA 1 cut(s) 78
CfoI GCGC 1 cut(s) 809
Cfr10I RCCGGY 1 cut(s) 830
Cfr13I GGNCC 3 cut(s) 121, 556, 1483
CseI GACGC 2 cut(s) 1579, 1973
Csp6I GTAC 8 cut(s) 312, 390, 498, 542, 1291, 1327, 1813, 1852
CspAI ACCGGT 1 cut(s) 830
CspCI CAANNNNNGTGG 2 cut(s) 1461, 1496
CviAII CATG 9 cut(s) 79, 125, 143, 534, 1161, 1287, 1855, 1915, 1955
CviQI GTAC 8 cut(s) 312, 390, 498, 542, 1291, 1327, 1813, 1852
DdeI CTNAG 3 cut(s) 813, 1278, 1545
DpnI GATC 7 cut(s) 47, 723, 858, 1068, 1149, 1220, 1635
DpnII GATC 7 cut(s) 45, 721, 856, 1066, 1147, 1218, 1633
DraI TTTAAA 1 cut(s) 288
DraIII CACNNNGTG 1 cut(s) 387
DriI GACNNNNNGTC 1 cut(s) 847
EaeI YGGCCR 2 cut(s) 183, 1317
Eam1104I CTCTTC 3 cut(s) 429, 1248, 1523
Eam1105I GACNNNNNGTC 1 cut(s) 847
EarI CTCTTC 3 cut(s) 429, 1248, 1523
Ecl136II GAGCTC 1 cut(s) 817
Eco130I CCWWGG 2 cut(s) 951, 1286
Eco24I GRGCYC 1 cut(s) 819
Eco31I GGTCTC 1 cut(s) 69
Eco53kI GAGCTC 1 cut(s) 817
Eco57I CTGAAG 3 cut(s) 1263, 1530, 1643
EcoICRI GAGCTC 1 cut(s) 817
EcoRII CCWGG 4 cut(s) 558, 1131, 1314, 1840
EcoT14I CCWWGG 2 cut(s) 951, 1286
EcoT38I GRGCYC 1 cut(s) 819
ErhI CCWWGG 2 cut(s) 951, 1286
FaeI CATG 9 cut(s) 82, 128, 146, 537, 1164, 1290, 1858, 1918, 1958
FalI AAGNNNNNCTT 2 cut(s) 495, 527
FaqI GGGAC 1 cut(s) 921
FatI CATG 9 cut(s) 78, 124, 142, 533, 1160, 1286, 1854, 1914, 1954
FauI CCCGC 1 cut(s) 1078
FauNDI CATATG 1 cut(s) 853
FbaI TGATCA 1 cut(s) 1147
Fnu4HI GCNGC 4 cut(s) 217, 448, 1049, 1337
FokI GGATG 3 cut(s) 25, 677, 875
FriOI GRGCYC 1 cut(s) 819
Fsp4HI GCNGC 4 cut(s) 217, 448, 1049, 1337
FspBI CTAG 3 cut(s) 159, 710, 1103
FspI TGCGCA 1 cut(s) 808
GlaI GCGC 1 cut(s) 808
GluI GCNGC 4 cut(s) 217, 448, 1049, 1337
GsuI CTGGAG 1 cut(s) 238
HaeIII GGCC 7 cut(s) 123, 185, 557, 950, 956, 1319, 1484
HapII CCGG 2 cut(s) 406, 831
HgaI GACGC 2 cut(s) 1579, 1973
HhaI GCGC 1 cut(s) 809
Hin1I GRCGYC 2 cut(s) 1571, 1965
Hin1II CATG 9 cut(s) 82, 128, 146, 537, 1164, 1290, 1858, 1918, 1958
Hin6I GCGC 1 cut(s) 807
HinP1I GCGC 1 cut(s) 807
HincII GTYRAC 2 cut(s) 599, 664
HindII GTYRAC 2 cut(s) 599, 664
HindIII AAGCTT 1 cut(s) 899
HinfI GANTC 5 cut(s) 179, 306, 491, 1185, 1420
HpaII CCGG 2 cut(s) 406, 831
HphI GGTGA 8 cut(s) 35, 41, 50, 485, 845, 1384, 1909, 1930
Hpy166II GTNNAC 7 cut(s) 41, 264, 390, 599, 664, 734, 1815
Hpy188I TCNGA 6 cut(s) 165, 1013, 1147, 1425, 1654, 1731
Hpy188III TCNNGA 7 cut(s) 79, 113, 154, 725, 993, 1151, 1583
Hpy8I GTNNAC 7 cut(s) 41, 264, 390, 599, 664, 734, 1815
Hpy99I CGWCG 2 cut(s) 1573, 1658
HpyAV CCTTC 4 cut(s) 1008, 1238, 1346, 1589
HpyCH4III ACNGT 2 cut(s) 107, 731
HpyCH4IV ACGT 3 cut(s) 496, 1458, 1656
HpyCH4V TGCA 5 cut(s) 95, 264, 450, 638, 1300
HpyF10VI GCNNNNNNNGC 3 cut(s) 129, 569, 1439
HpyF3I CTNAG 3 cut(s) 813, 1278, 1545
HpySE526I ACGT 3 cut(s) 496, 1458, 1656
Hsp92I GRCGYC 2 cut(s) 1571, 1965
Hsp92II CATG 9 cut(s) 82, 128, 146, 537, 1164, 1290, 1858, 1918, 1958
HspAI GCGC 1 cut(s) 807
KpnI GGTACC 2 cut(s) 315, 1330
Ksp22I TGATCA 1 cut(s) 1147
Kzo9I GATC 7 cut(s) 45, 721, 856, 1066, 1147, 1218, 1633
LguI GCTCTTC 2 cut(s) 1248, 1523
LmnI GCTCC 2 cut(s) 257, 1753
Lsp1109I GCAGC 4 cut(s) 228, 434, 1060, 1348
LweI GCATC 6 cut(s) 625, 1065, 1272, 1555, 1794, 1854
MaeI CTAG 3 cut(s) 159, 710, 1103
MaeII ACGT 3 cut(s) 496, 1458, 1656
MaeIII GTNAC 4 cut(s) 292, 672, 1644, 1673
MalI GATC 7 cut(s) 47, 723, 858, 1068, 1149, 1220, 1635
MboI GATC 7 cut(s) 45, 721, 856, 1066, 1147, 1218, 1633
MboII GAAGA 8 cut(s) 416, 515, 596, 1265, 1540, 1679, 1725, 1813
MfeI CAATTG 1 cut(s) 1201
MflI RGATCY 2 cut(s) 1218, 1633
MhlI GDGCHC 3 cut(s) 266, 690, 819
MlsI TGGCCA 1 cut(s) 1319
MluCI AATT 9 cut(s) 100, 281, 343, 358, 740, 753, 996, 1201, 1870
MluNI TGGCCA 1 cut(s) 1319
MmeI TCCRAC 3 cut(s) 319, 681, 1677
Mox20I TGGCCA 1 cut(s) 1319
MscI TGGCCA 1 cut(s) 1319
MslI CAYNNNNRTG 2 cut(s) 1097, 1822
Msp20I TGGCCA 1 cut(s) 1319
MspA1I CMGCKG 3 cut(s) 219, 1339, 1889
MspI CCGG 2 cut(s) 406, 831
MspR9I CCNGG 5 cut(s) 406, 560, 1133, 1316, 1842
MunI CAATTG 1 cut(s) 1201
Mva1269I GAATGC 1 cut(s) 1510
MvaI CCWGG 4 cut(s) 560, 1133, 1316, 1842
MvnI CGCG 1 cut(s) 1295
MwoI GCNNNNNNNGC 3 cut(s) 129, 569, 1439
NciI CCSGG 1 cut(s) 406
NcoI CCATGG 1 cut(s) 1286
NdeI CATATG 1 cut(s) 853
NdeII GATC 7 cut(s) 45, 721, 856, 1066, 1147, 1218, 1633
NlaIII CATG 9 cut(s) 82, 128, 146, 537, 1164, 1290, 1858, 1918, 1958
NlaIV GGNNCC 5 cut(s) 313, 1220, 1328, 1743, 1763
NmeAIII GCCGAG 1 cut(s) 982
NmuCI GTSAC 1 cut(s) 292
NsbI TGCGCA 1 cut(s) 808
NspV TTCGAA 1 cut(s) 1350
PagI TCATGA 1 cut(s) 78
PciSI GCTCTTC 2 cut(s) 1248, 1523
PctI GAATGC 1 cut(s) 1510
PfeI GAWTC 5 cut(s) 179, 306, 491, 1185, 1420
Pfl23II CGTACG 1 cut(s) 497
PinAI ACCGGT 1 cut(s) 830
PkrI GCNGC 4 cut(s) 218, 449, 1050, 1338
Ppu21I YACGTR 1 cut(s) 497
PshAI GACNNNNGTC 1 cut(s) 1574
PshBI ATTAAT 2 cut(s) 204, 1095
Psp124BI GAGCTC 1 cut(s) 819
Psp1406I AACGTT 1 cut(s) 1458
Psp6I CCWGG 4 cut(s) 558, 1131, 1314, 1840
PspGI CCWGG 4 cut(s) 558, 1131, 1314, 1840
PspLI CGTACG 1 cut(s) 497
PspN4I GGNNCC 5 cut(s) 313, 1220, 1328, 1743, 1763
PspPI GGNCC 3 cut(s) 121, 556, 1483
PsuI RGATCY 2 cut(s) 1218, 1633
RsaI GTAC 8 cut(s) 313, 391, 499, 543, 1292, 1328, 1814, 1853
RsaNI GTAC 8 cut(s) 312, 390, 498, 542, 1291, 1327, 1813, 1852
RseI CAYNNNNRTG 2 cut(s) 1097, 1822
SacI GAGCTC 1 cut(s) 819
SapI GCTCTTC 2 cut(s) 1248, 1523
SatI GCNGC 4 cut(s) 217, 448, 1049, 1337
Sau3AI GATC 7 cut(s) 45, 721, 856, 1066, 1147, 1218, 1633
Sau96I GGNCC 3 cut(s) 121, 556, 1483
ScrFI CCNGG 5 cut(s) 406, 560, 1133, 1316, 1842
SduI GDGCHC 3 cut(s) 266, 690, 819
SfaNI GCATC 6 cut(s) 625, 1065, 1272, 1555, 1794, 1854
SfuI TTCGAA 1 cut(s) 1350
SmiMI CAYNNNNRTG 2 cut(s) 1097, 1822
SmlI CTYRAG 5 cut(s) 316, 695, 902, 1331, 1784
SmoI CTYRAG 5 cut(s) 316, 695, 902, 1331, 1784
SpeI ACTAGT 2 cut(s) 158, 709
Sse9I AATT 9 cut(s) 100, 281, 343, 358, 740, 753, 996, 1201, 1870
SsiI CCGC 6 cut(s) 219, 1046, 1071, 1295, 1339, 1889
SspI AATATT 2 cut(s) 202, 1304
SspMI CTAG 3 cut(s) 159, 710, 1103
SstI GAGCTC 1 cut(s) 819
StyD4I CCNGG 5 cut(s) 404, 558, 1131, 1314, 1840
StyI CCWWGG 2 cut(s) 951, 1286
TaaI ACNGT 2 cut(s) 107, 731
TaiI ACGT 3 cut(s) 499, 1461, 1659
TasI AATT 9 cut(s) 100, 281, 343, 358, 740, 753, 996, 1201, 1870
TatI WGTACW 2 cut(s) 541, 1851
TfiI GAWTC 5 cut(s) 179, 306, 491, 1185, 1420
TscAI CASTG 6 cut(s) 102, 736, 991, 1066, 1327, 1763
TseFI GTSAC 1 cut(s) 292
TseI GCWGC 4 cut(s) 216, 447, 1048, 1336
Tsp45I GTSAC 1 cut(s) 292
TspGWI ACGGA 4 cut(s) 58, 515, 1094, 1451
TspRI CASTG 6 cut(s) 102, 736, 991, 1066, 1327, 1763
VneI GTGCAC 1 cut(s) 262
VspI ATTAAT 2 cut(s) 204, 1095
XapI RAATTY 2 cut(s) 281, 358
XcmI CCANNNNNNNNNTGG 1 cut(s) 1493
XspI CTAG 3 cut(s) 159, 710, 1103
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.