MD13G1206000.v1.1

Sieve element occlusion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0000511
Physical Location & Seq
Forward (+)
18719748 .. 18723375
3628 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1206000.v1.1.491

Sequence Viewer

Length: 2049 bp
ATGCTAGGTCTAGCAAACAATGTGGCTGCCAAGGTGGCCTCAGTAGTTACCACTACTCATCAACACATTACTGGCGATCATGAGCTTAGCCTATTTACCATGTCTGACCAGAAAATCTTGGAACAAATTTATGGTACTCATGTTCATGCTGATGAATCCTTTGATGATGATTCTCTTTTCGGCATCACCGAAAATATCCTTAAGCGTGCTACCCAAATTGTTGACAAAATTGTGCAGGGTACCCAAGTGCACGTGGAGAACATAGAGGAGAACACACCCAAAGCAGGCTTCAGTGCACCATTGTGCACACTAAAGTCCATAGCCAGCGAGATGCAATGCAAGCCTCCTAGTGAGGAAGTTGCCCACAACACAACCCTGGCCATACTGAACAAGCTATCAAGCTATTCATGGGAAGCAAAGGCAGTGCTGACTCTAGCAGCTTTTGCCATGGAATACGGGGAGTTCTGGCTCCTCGCCCAGCTTCAAGAATCAGACCGACTCGCCAAATCAATTGCAATCCTGAAGCGAGTCCCCGTGCTCCTCAAGCCCTCGGACCTGCACAAGAAAAGACAAGCAGTGCTTGAACTTAACAATCTGATCAAGGCCACATTGCAAGTGATTGAGTGCATCGACCAATTCGATAAGCTTTCCAGCTACGACCCAAAAGATGTCCCAGCGTTGGCGCTAGCAATGGACCATATTCCAGTTGATGTGTACTGGGCTGTTGCCACTGTTGTTGCTTGTGCAACTAAGATCACCATCCTCACCTGTAATGAGGACAAGGAGCATGATCTAGCCCCATTTGCTCAAAAAATTCACTATGTCCTAAACAAGCTTAAGATCCAGCTTATTGTCTGCAGAAAACAAATTGAGGAGGCAGAGACTTACCGGAGGCTAAGGAAAATCTTCCGAACTCCTACTGAAATCATGGAGGTTTTCAAGGCCCTGATTTTTACCAAGGAAAATGTCCAGCCGCTCGTTGATGGTTCTACTAAACAAATGGTTAAAATCGATATCCTGAGGAAGAAGAACGTGTTGTTGTTCATTTCAAGCCTAGACATCTCTGATGATGATATCTCAATCCTCAAGCCCATCTATGATATGATAAAGAAAGACAATCAGCACAAGATTGTGTGGATTCCGATTGTGGAGCATTGGACCGATGACCGCCGAAAGAAGTTTGAGAGCCTCCGAAATAAGATGCCATGGTACACAGTGCAGATCTCGGCACCTGTAGCAGGCATCAGATTCATCAAGGAGGAGTGGAGCTTCAAGGGTAAGCCCACACTAGTGGTGATGAACCCACAAGGAAAGGTCGAACATCCCAACGCTCTCCACATGATTAGGGTTTGGGGAGTCAATGCTTTCCCTTTCACAAAGGCAACTGAAGAAGAGCTCTCACATGGTCACGGAGATAAATGGATTGGCACTGTTGTCCAAGGGGTTAGCCAATCTGTTACGATCAAAGAGGACAAGTATATTTTCTTCTATGGTGGCAAAGACAATGGTTGGATTCAAGAGTTCACAAAGAAAGCAACTGCCCTTGCAAATGACCCCATCTTCAAGGAAGCAAAGATTCAAATCGAGTTGTTCTGCGTGGGAAAAGGAAGCAAGGGGGAAGATGACCATGGCATCCTCGGGAAGTTCTGGACTGGCATCGAAAGCTTGTTCTTCACCAAGGTTCACAAGCCAGCCGACCAAGTCGGTCAAGAAATCCAGAAGCTACTTTCTTACAAAAACGAAAGTGGATGGGCTGTGCTCAGCAAAGGGCATTCTGTGGTGCTCACCGGCCACGGTGTCTCGATCTTGAGGGTGGTAGAGGACTTTGACAAATGGAAGGACCATGTGAAAGAGAGTGGCTTTGAGTTTTGCTTCAAGTCATACCATGAGAGGGTTCGCACTGTGAGTCGACCTTGCTGCCGCCTTGATATTCCAGGCTCTACCGGGAAGGTTCCAGACACCATGAAGTGTCCTGATTGCCACCGGAGCATGGAGACTTTCATCAGTTACAAGTGTTGCCACATTGATGGTCCTACTGCACATCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SEO_C SEO_N

Protein Analysis

683

Amino Acids

77.19

Weight (kDa)

7.35

Isoelectric Point (pI)

36.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SEO_N PF14576 35 - 306 9.1e-89 Sieve element occlusion N-terminus
SEO_C PF14577 484 - 541 9e-09 Sieve element occlusion C-terminus
SEO_C PF14577 549 - 674 1.8e-33 Sieve element occlusion C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000511)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g02370 FvH4_4g02370 FvH4_4g02370 FvH4_4g02430 FvH4_4g02430 FvH4_4g02460 FvH4_4g02460 FvH4_4g02460 FvH4_4g02480 FvH4_4g02480 FvH4_4g02480 FvH4_4g02480 FvH4_6g05920
malus_domestica MD00G1111000.v1.1 MD08G1225800.v1.1 MD13G1205700.v1.1 MD13G1205800.v1.1 MD13G1205900.v1.1 MD13G1206000.v1.1 MD16G1207500.v1.1 MD16G1207600.v1.1
prunus_persica Prupe.1G028400_v2.0.a1 Prupe.1G028500_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028900_v2.0.a1 Prupe.1G029000_v2.0.a1 Prupe.1G029100_v2.0.a1
pyrus_communis pycom08g19680 pycom08g19730 pycom16g17460 pycom16g17500
rosa_chinensis RchiOBHm_Chr4g0389751 RchiOBHm_Chr4g0389771 RchiOBHm_Chr4g0389791 RchiOBHm_Chr4g0389861 RchiOBHm_Chr4g0389881 RchiOBHm_Chr4g0389901 RchiOBHm_Chr4g0412411
rosa_laevigata RLG00000008288 RLG00000009901 RLG00000009945 RLG00000009954 RLG00000009956 RLG00000009957 RLG00000009958 RLG00000019246
rosa_multiflora Rmu_co8275125.1_g000001 Rmu_sc0002083.1_g000003 Rmu_sc0002083.1_g000025 Rmu_sc0002604.1_g000016 Rmu_sc0004336.1_g000031 Rmu_sc0010423.1_g000003
rosa_roxburghii Rroxscaffold_5G00335790 Rroxscaffold_5G00335850 Rroxscaffold_5G00335970 Rroxscaffold_5G00335990
rosa_rugosa Rorug03G0325100 Rorug03G0325300 Rorug03G0325500.1 Rorug03G0326800 Rorug03G0327000 Rorug04G0104400
rosa_samantha Rh4AG029900 Rh4AG030000 Rh4AG030100 Rh4AG030200 Rh4AG030600 Rh4AG172900 Rh4BG023200 Rh4BG023900 Rh4BG024000 Rh4BG024100 Rh4BG172200 Rh4DG023300 Rh4DG023800 Rh4DG023900 Rh4DG024000 Rh4DG151600
rosa_wichuraiana Rw1G002330 Rw1G002340 Rw4G002260 Rw4G002270 Rw4G002280 Rw4G002300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 564
Acc65I GGTACC 1 cut(s) 239
AccB1I GGYRCC 2 cut(s) 239, 1228
AccBSI CCGCTC 1 cut(s) 976
AccI GTMKAC 1 cut(s) 1909
AciI CCGC 3 cut(s) 974, 1168, 1921
AclWI GGATC 1 cut(s) 835
AcoI YGGCCR 2 cut(s) 378, 1789
AcsI RAATTY 2 cut(s) 126, 813
AcuI CTGAAG 3 cut(s) 274, 542, 1407
AcvI CACGTG 1 cut(s) 253
AfaI GTAC 4 cut(s) 136, 241, 716, 1211
AfiI CCNNNNNNNGG 5 cut(s) 284, 679, 1238, 1891, 1990
AflII CTTAAG 2 cut(s) 200, 836
AflIII ACRYGT 1 cut(s) 1032
AgsI TTSAA 9 cut(s) 485, 584, 940, 1050, 1273, 1517, 1564, 1580, 1876
AhlI ACTAGT 1 cut(s) 1288
AjnI CCWGG 2 cut(s) 375, 1933
AjuI GAANNNNNNNTTGG 2 cut(s) 272, 304
AleI CACNNNNGTG 2 cut(s) 301, 1289
Alw21I GWGCWC 7 cut(s) 252, 298, 308, 540, 1398, 1761, 1785
Alw26I GTCTC 3 cut(s) 875, 1804, 1988
Alw44I GTGCAC 3 cut(s) 248, 294, 304
AlwI GGATC 1 cut(s) 835
Ama87I CYCGRG 1 cut(s) 1637
AoxI GGCC 5 cut(s) 36, 378, 603, 942, 1789
ApaLI GTGCAC 3 cut(s) 248, 294, 304
ApeKI GCWGC 3 cut(s) 26, 437, 1917
ApoI RAATTY 2 cut(s) 126, 813
Asp700I GAANNNNTTC 1 cut(s) 905
Asp718I GGTACC 1 cut(s) 239
AspLEI GCGC 1 cut(s) 685
AspS9I GGNCC 6 cut(s) 553, 694, 943, 1158, 1840, 2030
AsuC2I CCSGG 1 cut(s) 1945
AsuHPI GGTGA 6 cut(s) 178, 748, 757, 1306, 1666, 1777
AsuNHI GCTAGC 1 cut(s) 685
AvaI CYCGRG 1 cut(s) 1637
AvaII GGWCC 5 cut(s) 553, 694, 1158, 1840, 2030
AxyI CCTNAGG 1 cut(s) 1019
BaeGI GKGCMC 3 cut(s) 252, 298, 308
BaeI ACNNNNGTAYC 4 cut(s) 126, 159, 231, 264
BalI TGGCCA 1 cut(s) 380
BanI GGYRCC 2 cut(s) 239, 1228
BanII GRGCYC 1 cut(s) 1398
BarI GAAGNNNNNNTAC 2 cut(s) 1469, 1501
BbrPI CACGTG 1 cut(s) 253
Bbv12I GWGCWC 7 cut(s) 252, 298, 308, 540, 1398, 1761, 1785
BbvI GCAGC 3 cut(s) 13, 449, 1904
BccI CCATC 6 cut(s) 767, 977, 1100, 1565, 1743, 2021
BcgI CGANNNNNNTGC 2 cut(s) 1899, 1933
BciT130I CCWGG 2 cut(s) 377, 1935
BclI TGATCA 1 cut(s) 597
BcnI CCSGG 1 cut(s) 1945
BcoDI GTCTC 3 cut(s) 875, 1804, 1988
BcuI ACTAGT 1 cut(s) 1288
BfaI CTAG 8 cut(s) 5, 11, 348, 434, 686, 794, 1055, 1289
BfmI CTRYAG 2 cut(s) 856, 1233
BfoI RGCGCY 1 cut(s) 686
BfrI CTTAAG 2 cut(s) 200, 836
BfuAI ACCTGC 1 cut(s) 564
BglI GCCNNNNNGGC 1 cut(s) 35
BglII AGATCT 1 cut(s) 1221
BisI GCNGC 5 cut(s) 27, 438, 974, 1918, 1921
BlpI GCTNAGC 2 cut(s) 86, 1760
BlsI GCNGC 5 cut(s) 28, 439, 975, 1919, 1922
Bme1390I CCNGG 3 cut(s) 377, 1935, 1945
Bme18I GGWCC 5 cut(s) 553, 694, 1158, 1840, 2030
BmeT110I CYCGRG 1 cut(s) 1637
BmgT120I GGNCC 6 cut(s) 553, 694, 943, 1158, 1840, 2030
BmiI GGNNCC 4 cut(s) 241, 470, 1230, 1953
BmrFI CCNGG 3 cut(s) 377, 1935, 1945
BmrI ACTGGG 1 cut(s) 727
BmsI GCATC 7 cut(s) 192, 321, 636, 1191, 1251, 1641, 1665
BmtI GCTAGC 1 cut(s) 689
BmuI ACTGGG 1 cut(s) 727
Bpu10I CCTNAGC 1 cut(s) 896
Bpu1102I GCTNAGC 2 cut(s) 86, 1760
BpuEI CTTGAG 3 cut(s) 527, 1070, 1828
BpuMI CCSGG 1 cut(s) 1945
Bsa29I ATCGAT 1 cut(s) 1011
BsaAI YACGTR 1 cut(s) 253
BsaBI GATNNNNATC 2 cut(s) 758, 1580
BsaWI WCCGGW 2 cut(s) 888, 1983
Bsc4I CCNNNNNNNGG 5 cut(s) 284, 679, 1238, 1891, 1990
Bse118I RCCGGY 1 cut(s) 1787
Bse1I ACTGG 4 cut(s) 76, 704, 722, 1657
Bse21I CCTNAGG 1 cut(s) 1019
Bse3DI GCAATG 3 cut(s) 341, 608, 696
Bse8I GATNNNNATC 2 cut(s) 758, 1580
BseBI CCWGG 2 cut(s) 377, 1935
BseCI ATCGAT 1 cut(s) 1011
BseGI GGATG 4 cut(s) 759, 1321, 1632, 1754
BseJI GATNNNNATC 2 cut(s) 758, 1580
BseLI CCNNNNNNNGG 5 cut(s) 284, 679, 1238, 1891, 1990
BseMI GCAATG 3 cut(s) 341, 608, 696
BseMII CTCAG 3 cut(s) 54, 1010, 1774
BseNI ACTGG 4 cut(s) 76, 704, 722, 1657
BseRI GAGGAG 5 cut(s) 281, 461, 530, 887, 1274
BseSI GKGCMC 3 cut(s) 252, 298, 308
BseXI GCAGC 3 cut(s) 13, 449, 1904
BseYI CCCAGC 2 cut(s) 477, 673
BsgI GTGCAG 4 cut(s) 254, 542, 1238, 2022
BshFI GGCC 5 cut(s) 38, 380, 605, 944, 1791
BshNI GGYRCC 2 cut(s) 239, 1228
BshVI ATCGAT 1 cut(s) 1011
BsiHKAI GWGCWC 7 cut(s) 252, 298, 308, 540, 1398, 1761, 1785
BsiHKCI CYCGRG 1 cut(s) 1637
BsiSI CCGG 4 cut(s) 889, 1788, 1944, 1984
BslFI GGGAC 2 cut(s) 515, 656
BslI CCNNNNNNNGG 5 cut(s) 284, 679, 1238, 1891, 1990
BsmAI GTCTC 3 cut(s) 875, 1804, 1988
BsmFI GGGAC 2 cut(s) 515, 656
BsmI GAATGC 1 cut(s) 1771
BsnI GGCC 5 cut(s) 38, 380, 605, 944, 1791
BsoBI CYCGRG 1 cut(s) 1637
Bsp1286I GDGCHC 7 cut(s) 252, 298, 308, 540, 1398, 1761, 1785
Bsp143I GATC 8 cut(s) 76, 597, 753, 790, 840, 1221, 1461, 1803
Bsp1720I GCTNAGC 2 cut(s) 86, 1760
Bsp19I CCATGG 3 cut(s) 447, 1205, 1627
BspACI CCGC 3 cut(s) 974, 1168, 1921
BspANI GGCC 5 cut(s) 38, 380, 605, 944, 1791
BspCNI CTCAG 3 cut(s) 53, 1011, 1773
BspDI ATCGAT 1 cut(s) 1011
BspHI TCATGA 1 cut(s) 79
BspLI GGNNCC 4 cut(s) 241, 470, 1230, 1953
BspMAI CTGCAG 1 cut(s) 860
BspMI ACCTGC 1 cut(s) 564
BspOI GCTAGC 1 cut(s) 689
BspPI GGATC 1 cut(s) 835
BspQI GCTCTTC 1 cut(s) 1386
BspT107I GGYRCC 2 cut(s) 239, 1228
BspTI CTTAAG 2 cut(s) 200, 836
BsrBI CCGCTC 1 cut(s) 976
BsrDI GCAATG 3 cut(s) 341, 608, 696
BsrFI RCCGGY 1 cut(s) 1787
BsrI ACTGG 4 cut(s) 76, 704, 722, 1657
BssAI RCCGGY 1 cut(s) 1787
BssMI GATC 8 cut(s) 76, 597, 753, 790, 840, 1221, 1461, 1803
BssT1I CCWWGG 7 cut(s) 30, 447, 957, 1205, 1438, 1627, 1677
Bst2UI CCWGG 2 cut(s) 377, 1935
Bst4CI ACNGT 5 cut(s) 733, 1216, 1432, 1796, 1903
Bst6I CTCTTC 1 cut(s) 1386
BstAFI CTTAAG 2 cut(s) 200, 836
BstAPI GCANNNNNTGC 1 cut(s) 443
BstBAI YACGTR 1 cut(s) 253
BstC8I GCNNGC 7 cut(s) 207, 286, 325, 341, 687, 1240, 1692
BstDEI CTNAG 6 cut(s) 40, 86, 750, 896, 1019, 1760
BstDSI CCRYGG 4 cut(s) 447, 1205, 1627, 1792
BstENI CCTNNNNNAGG 1 cut(s) 1236
BstF5I GGATG 4 cut(s) 759, 1321, 1632, 1754
BstH2I RGCGCY 1 cut(s) 686
BstHHI GCGC 1 cut(s) 685
BstKTI GATC 8 cut(s) 79, 600, 756, 793, 843, 1224, 1464, 1806
BstMAI GTCTC 3 cut(s) 875, 1804, 1988
BstMBI GATC 8 cut(s) 76, 597, 753, 790, 840, 1221, 1461, 1803
BstMWI GCNNNNNNNGC 8 cut(s) 35, 340, 443, 544, 803, 1235, 1662, 1986
BstNI CCWGG 2 cut(s) 377, 1935
BstSCI CCNGG 3 cut(s) 375, 1933, 1943
BstSFI CTRYAG 2 cut(s) 856, 1233
BstSLI GKGCMC 3 cut(s) 252, 298, 308
BstV1I GCAGC 3 cut(s) 13, 449, 1904
BstX2I RGATCY 2 cut(s) 840, 1221
BstXI CCANNNNNNTGG 2 cut(s) 1291, 2027
BstYI RGATCY 2 cut(s) 840, 1221
Bsu15I ATCGAT 1 cut(s) 1011
Bsu36I CCTNAGG 1 cut(s) 1019
BsuRI GGCC 5 cut(s) 38, 380, 605, 944, 1791
BsuTUI ATCGAT 1 cut(s) 1011
BtgI CCRYGG 4 cut(s) 447, 1205, 1627, 1792
BtsCI GGATG 4 cut(s) 759, 1321, 1632, 1754
BtsI GCAGTG 2 cut(s) 429, 582
BtsIMutI CAGTG 7 cut(s) 298, 429, 582, 729, 1221, 1428, 1899
BveI ACCTGC 1 cut(s) 564
Cac8I GCNNGC 7 cut(s) 207, 286, 325, 341, 687, 1240, 1692
CciI TCATGA 1 cut(s) 79
CfoI GCGC 1 cut(s) 685
Cfr10I RCCGGY 1 cut(s) 1787
Cfr13I GGNCC 6 cut(s) 553, 694, 943, 1158, 1840, 2030
ClaI ATCGAT 1 cut(s) 1011
Csp6I GTAC 4 cut(s) 135, 240, 715, 1210
CspCI CAANNNNNGTGG 3 cut(s) 38, 718, 753
CviQI GTAC 4 cut(s) 135, 240, 715, 1210
DdeI CTNAG 6 cut(s) 40, 86, 750, 896, 1019, 1760
DpnI GATC 8 cut(s) 78, 599, 755, 792, 842, 1223, 1463, 1805
DpnII GATC 8 cut(s) 76, 597, 753, 790, 840, 1221, 1461, 1803
EaeI YGGCCR 2 cut(s) 378, 1789
Eam1104I CTCTTC 1 cut(s) 1386
EarI CTCTTC 1 cut(s) 1386
Ecl136II GAGCTC 1 cut(s) 1396
Eco130I CCWWGG 7 cut(s) 30, 447, 957, 1205, 1438, 1627, 1677
Eco24I GRGCYC 1 cut(s) 1398
Eco32I GATATC 2 cut(s) 1015, 1075
Eco47I GGWCC 5 cut(s) 553, 694, 1158, 1840, 2030
Eco53kI GAGCTC 1 cut(s) 1396
Eco57I CTGAAG 3 cut(s) 274, 542, 1407
Eco72I CACGTG 1 cut(s) 253
Eco81I CCTNAGG 1 cut(s) 1019
Eco88I CYCGRG 1 cut(s) 1637
EcoICRI GAGCTC 1 cut(s) 1396
EcoNI CCTNNNNNAGG 1 cut(s) 1236
EcoO109I RGGNCCY 1 cut(s) 943
EcoRII CCWGG 2 cut(s) 375, 1933
EcoRV GATATC 2 cut(s) 1015, 1075
EcoT14I CCWWGG 7 cut(s) 30, 447, 957, 1205, 1438, 1627, 1677
EcoT38I GRGCYC 1 cut(s) 1398
ErhI CCWWGG 7 cut(s) 30, 447, 957, 1205, 1438, 1627, 1677
FalI AAGNNNNNCTT 2 cut(s) 564, 596
FaqI GGGAC 2 cut(s) 515, 656
FbaI TGATCA 1 cut(s) 597
FblI GTMKAC 1 cut(s) 1909
Fnu4HI GCNGC 5 cut(s) 27, 438, 974, 1918, 1921
FokI GGATG 4 cut(s) 746, 1308, 1619, 1761
FriOI GRGCYC 1 cut(s) 1398
Fsp4HI GCNGC 5 cut(s) 27, 438, 974, 1918, 1921
FspBI CTAG 8 cut(s) 5, 11, 348, 434, 686, 794, 1055, 1289
GlaI GCGC 1 cut(s) 684
GluI GCNGC 5 cut(s) 27, 438, 974, 1918, 1921
GsaI CCCAGC 2 cut(s) 481, 677
HaeII RGCGCY 1 cut(s) 686
HaeIII GGCC 5 cut(s) 38, 380, 605, 944, 1791
HapII CCGG 4 cut(s) 889, 1788, 1944, 1984
HhaI GCGC 1 cut(s) 685
Hin6I GCGC 1 cut(s) 683
HinP1I GCGC 1 cut(s) 683
HincII GTYRAC 2 cut(s) 223, 1910
HindII GTYRAC 2 cut(s) 223, 1910
HindIII AAGCTT 3 cut(s) 644, 833, 1663
HpaII CCGG 4 cut(s) 889, 1788, 1944, 1984
HphI GGTGA 6 cut(s) 178, 748, 757, 1306, 1666, 1777
Hpy166II GTNNAC 9 cut(s) 223, 250, 296, 306, 715, 1212, 1524, 1684, 1910
Hpy188I TCNGA 9 cut(s) 106, 493, 553, 597, 911, 1066, 1143, 1193, 1247
Hpy8I GTNNAC 9 cut(s) 223, 250, 296, 306, 715, 1212, 1524, 1684, 1910
HpyAV CCTTC 2 cut(s) 1831, 1942
HpyCH4III ACNGT 5 cut(s) 733, 1216, 1432, 1796, 1903
HpyCH4IV ACGT 2 cut(s) 252, 1032
HpyF10VI GCNNNNNNNGC 8 cut(s) 35, 340, 443, 544, 803, 1235, 1662, 1986
HpyF3I CTNAG 6 cut(s) 40, 86, 750, 896, 1019, 1760
HpySE526I ACGT 2 cut(s) 252, 1032
HspAI GCGC 1 cut(s) 683
KpnI GGTACC 1 cut(s) 243
Ksp22I TGATCA 1 cut(s) 597
Kzo9I GATC 8 cut(s) 76, 597, 753, 790, 840, 1221, 1461, 1803
LguI GCTCTTC 1 cut(s) 1386
LmnI GCTCC 6 cut(s) 474, 543, 784, 1150, 1266, 1986
Lsp1109I GCAGC 3 cut(s) 13, 449, 1904
LweI GCATC 7 cut(s) 192, 321, 636, 1191, 1251, 1641, 1665
MaeI CTAG 8 cut(s) 5, 11, 348, 434, 686, 794, 1055, 1289
MaeII ACGT 2 cut(s) 252, 1032
MaeIII GTNAC 4 cut(s) 46, 1406, 1456, 2006
MalI GATC 8 cut(s) 78, 599, 755, 792, 842, 1223, 1463, 1805
MbiI CCGCTC 1 cut(s) 976
MboI GATC 8 cut(s) 76, 597, 753, 790, 840, 1221, 1461, 1803
MboII GAAGA 9 cut(s) 898, 1036, 1039, 1400, 1403, 1477, 1552, 1631, 1663
MfeI CAATTG 1 cut(s) 510
MflI RGATCY 2 cut(s) 840, 1221
MhlI GDGCHC 7 cut(s) 252, 298, 308, 540, 1398, 1761, 1785
MlsI TGGCCA 1 cut(s) 380
MluCI AATT 7 cut(s) 126, 216, 228, 510, 635, 813, 867
MluNI TGGCCA 1 cut(s) 380
MlyI GAGTC 5 cut(s) 424, 492, 537, 1365, 1915
MmeI TCCRAC 1 cut(s) 1490
Mox20I TGGCCA 1 cut(s) 380
MroXI GAANNNNTTC 1 cut(s) 905
MscI TGGCCA 1 cut(s) 380
MseI TTAA 4 cut(s) 201, 588, 837, 1005
MslI CAYNNNNRTG 5 cut(s) 144, 150, 301, 1289, 2025
Msp20I TGGCCA 1 cut(s) 380
MspCI CTTAAG 2 cut(s) 200, 836
MspI CCGG 4 cut(s) 889, 1788, 1944, 1984
MspR9I CCNGG 3 cut(s) 377, 1935, 1945
MunI CAATTG 1 cut(s) 510
Mva1269I GAATGC 1 cut(s) 1771
MvaI CCWGG 2 cut(s) 377, 1935
MwoI GCNNNNNNNGC 8 cut(s) 35, 340, 443, 544, 803, 1235, 1662, 1986
NciI CCSGG 1 cut(s) 1945
NcoI CCATGG 3 cut(s) 447, 1205, 1627
NdeII GATC 8 cut(s) 76, 597, 753, 790, 840, 1221, 1461, 1803
NheI GCTAGC 1 cut(s) 685
NlaIV GGNNCC 4 cut(s) 241, 470, 1230, 1953
NmeAIII GCCGAG 1 cut(s) 1205
NmuCI GTSAC 1 cut(s) 1406
OliI CACNNNNGTG 2 cut(s) 301, 1289
PagI TCATGA 1 cut(s) 79
PciSI GCTCTTC 1 cut(s) 1386
PcsI WCGNNNNNNNCGW 2 cut(s) 186, 636
PctI GAATGC 1 cut(s) 1771
PdmI GAANNNNTTC 1 cut(s) 905
PfeI GAWTC 7 cut(s) 155, 170, 488, 1138, 1248, 1513, 1576
PflFI GACNNNGTC 1 cut(s) 1700
PkrI GCNGC 5 cut(s) 28, 439, 975, 1919, 1922
PleI GAGTC 5 cut(s) 424, 492, 536, 1364, 1914
PmaCI CACGTG 1 cut(s) 253
PmlI CACGTG 1 cut(s) 253
PpsI GAGTC 5 cut(s) 424, 492, 536, 1364, 1914
Ppu21I YACGTR 1 cut(s) 253
Psp124BI GAGCTC 1 cut(s) 1398
Psp6I CCWGG 2 cut(s) 375, 1933
PspCI CACGTG 1 cut(s) 253
PspFI CCCAGC 2 cut(s) 477, 673
PspGI CCWGG 2 cut(s) 375, 1933
PspN4I GGNNCC 4 cut(s) 241, 470, 1230, 1953
PspPI GGNCC 6 cut(s) 553, 694, 943, 1158, 1840, 2030
PstI CTGCAG 1 cut(s) 860
PsuI RGATCY 2 cut(s) 840, 1221
PsyI GACNNNGTC 1 cut(s) 1700
RsaI GTAC 4 cut(s) 136, 241, 716, 1211
RsaNI GTAC 4 cut(s) 135, 240, 715, 1210
RseI CAYNNNNRTG 5 cut(s) 144, 150, 301, 1289, 2025
SacI GAGCTC 1 cut(s) 1398
SalI GTCGAC 1 cut(s) 1908
SapI GCTCTTC 1 cut(s) 1386
SaqAI TTAA 4 cut(s) 201, 588, 837, 1005
SatI GCNGC 5 cut(s) 27, 438, 974, 1918, 1921
Sau3AI GATC 8 cut(s) 76, 597, 753, 790, 840, 1221, 1461, 1803
Sau96I GGNCC 6 cut(s) 553, 694, 943, 1158, 1840, 2030
SchI GAGTC 5 cut(s) 424, 492, 537, 1365, 1915
ScrFI CCNGG 3 cut(s) 377, 1935, 1945
SduI GDGCHC 7 cut(s) 252, 298, 308, 540, 1398, 1761, 1785
SfaNI GCATC 7 cut(s) 192, 321, 636, 1191, 1251, 1641, 1665
SfcI CTRYAG 2 cut(s) 856, 1233
SinI GGWCC 5 cut(s) 553, 694, 1158, 1840, 2030
SmiMI CAYNNNNRTG 5 cut(s) 144, 150, 301, 1289, 2025
SmlI CTYRAG 5 cut(s) 200, 542, 836, 1085, 1807
SmoI CTYRAG 5 cut(s) 200, 542, 836, 1085, 1807
SpeI ACTAGT 1 cut(s) 1288
Sse9I AATT 7 cut(s) 126, 216, 228, 510, 635, 813, 867
SsiI CCGC 3 cut(s) 974, 1168, 1921
SspMI CTAG 8 cut(s) 5, 11, 348, 434, 686, 794, 1055, 1289
SstI GAGCTC 1 cut(s) 1398
StyD4I CCNGG 3 cut(s) 375, 1933, 1943
StyI CCWWGG 7 cut(s) 30, 447, 957, 1205, 1438, 1627, 1677
TaaI ACNGT 5 cut(s) 733, 1216, 1432, 1796, 1903
TaiI ACGT 2 cut(s) 255, 1035
TaqI TCGA 8 cut(s) 630, 639, 1011, 1317, 1584, 1659, 1802, 1909
TaqII GACCGA 3 cut(s) 510, 1175, 1694
TasI AATT 7 cut(s) 126, 216, 228, 510, 635, 813, 867
TatI WGTACW 1 cut(s) 714
TauI GCSGC 2 cut(s) 976, 1923
TfiI GAWTC 7 cut(s) 155, 170, 488, 1138, 1248, 1513, 1576
Tru1I TTAA 4 cut(s) 201, 588, 837, 1005
Tru9I TTAA 4 cut(s) 201, 588, 837, 1005
TscAI CASTG 7 cut(s) 298, 429, 582, 736, 1221, 1435, 1906
TseFI GTSAC 1 cut(s) 1406
TseI GCWGC 3 cut(s) 26, 437, 1917
Tsp45I GTSAC 1 cut(s) 1406
TspDTI ATGAA 8 cut(s) 134, 168, 396, 1033, 1240, 1313, 1979, 1990
TspGWI ACGGA 1 cut(s) 1425
TspRI CASTG 7 cut(s) 298, 429, 582, 736, 1221, 1435, 1906
Tth111I GACNNNGTC 1 cut(s) 1700
Vha464I CTTAAG 2 cut(s) 200, 836
VneI GTGCAC 3 cut(s) 248, 294, 304
VpaK11BI GGWCC 5 cut(s) 553, 694, 1158, 1840, 2030
XagI CCTNNNNNAGG 1 cut(s) 1236
XapI RAATTY 2 cut(s) 126, 813
XmiI GTMKAC 1 cut(s) 1909
XmnI GAANNNNTTC 1 cut(s) 905
XspI CTAG 8 cut(s) 5, 11, 348, 434, 686, 794, 1055, 1289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.