Rh4AG030200

Sieve element occlusion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000511
Physical Location & Seq
Forward (+)
6226130 .. 6229667
3538 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG030200.1

Sequence Viewer

Length: 2049 bp
ATGCTAGGCCTAGCACACAATGTGGCTACCAAGGTGGCCTCAGTAGTCCACACTACTCAGAAAACCATTGCTCGGGAGCTTAGCCTGTTTGCCATGCCTGATAAAAAGATCTTGGAGGAGATTTACACAACTCATGTCCACGCTGATGAATCCTTTGATGACGATTCTCTTTTCGTCATTGTTGAGAACATCCTCAAGCGTGCAACCCAAAATGTTGATAAAATAGTGCAGGGTACTCAAGTGCATGTGGACAACATAGAGGAGAAAACCCCCAAAGCCAGTTTCAGTGTACCATTGTGCACGCTAAAGGGAATCTCCTGCGAGATGCAATGCAAGCCTCCTGGAGATGAAATCGCCCACAACACAACAGTGGCAATACTCAACAAGCTGTCAAAGTATTCATGGGAAGCAAAGGCATCACTGACCCTAGCAGCTTTTGCTATGGAATATGGTGAATTCTGGCTCCTGGCGCAGCTTCGCGAATCAGACCACCTTGCCAAATCAATTGCAATCCTCAAACGAGTCCCTGTTCTCCTCCAGCCGTCAGAGCTGCACAAGCGCAGGCAATCAATTCTTGAGCTTAACAACCTGATCAAGGCCATACTGCAAGTGATCGAATGCATCGACCGGTTCAATAAGTATTCCACTTATGACCCAAAAGATGTCCCCGATTTGTCGATAGCCTTGGATCATATTCCGGTTGATGTGTATTGGGTTATCATCACTGTTGTTGCTTGTGCCACTAAGATCACTATACTCACTAGTGATGAGGACAAGGAGTATGATCTAGCCCCCTATTCTCAAAAAATTCACTATGTCCTAAACAAGCTAACGATTCAGCTAAGAGGTTGCAGGAAACAAATTGAGGAGGCAGAGACTTACAGAAGGATCACGAAATTGTTCCGAACCCCTACTGAAATTATGGAGGTTTTCAAGGGACTGATATTTACAAAGGATAATGTCCAGCCACTCATCGATGGTTCAACTAAGCAAACGGTTAATATCGACATCCTAAGGAGAAAGAATGTGTTATTGTTCATTTCCACCCTAGATGTCTCTGACGATGACATTTCGATTTTGAAACCGATCCACGAGTTCATAAAGAGAGACAATCAGTATAAGATTATCTGGATTCCAATTGTGGAGCAATGGACTGATGACCTGAGAAAAAAATTTGATATCCTGAAGAACAAAATGCCTTGGTTTACGGTGCAGTACTCGGGACCTATTGCAGGCATCAAGTTCATCAAGGAGGAGTGGAATTTCAAGGGTAAGCCTACGGTGGTGGTGATGAACCCACAAGGAAAGGTTGAACACTCCAACGCTCTCCACATGATTCGGGTATGGGGAGCCAAGGCCTTCCCTTTCACAAAGACTACAGAAGAAGAACTGTCACATTCTCATGCAGGAAAATGGGTTGGCTCCGTTGTTGAAGGAACTCACCCATCCGTGCACACTTGGATCAAAGAGGATAAGTATATCTTCTTCTATGGAGGTAAAGACAACGAATGGATTCAGCAATTCACAAAGAAAGCGACTACCCTTGCAAATGATCCAATCTTCAAGGAGGCAAAGATTCACCTAGAGTTGGTCTGCGTCGGAAAAGGCAGCAAAGGAGAAGACGACCATGGTGTTCTAGGCCGTTTCTGGACCAGTGTAGAGAGCCTGTTTTTCACCAAAGGCCACAAGCAGGTGGAGTCAGTGAATCAAGAAATCCAGAAGCTTCTCTCTTACAAAAACGAAAGTGGATGGGCGGTGCTTAGCAAAGGGTCGACTGTGTTGGTCACAGGTCATGGTGTCTCGGTGTTGAAGGTGGTTGAGGACTTTGAGAAATGGAAGGACCATGTCAAGGAGAAAGGGTTTGAGTTTTGTTTCAAGGCTTATCATGAGAGCATTAGTCAGGCTAGTCGACCTTGCTGCCGTCTGGACATTCCGGGCTCTAACGGAAAGGTTCCGGAGTCGATGAGGTGCCCTGATTGCCACCGTAATATGGAGACTTTTATCAGTTACAAGTGCTGCCACATTGATGGTCCTACTGGACATCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SEO_C SEO_N

Protein Analysis

682

Amino Acids

77.77

Weight (kDa)

7.6

Isoelectric Point (pI)

37.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SEO_N PF14576 34 - 304 4.9e-86 Sieve element occlusion N-terminus
SEO_C PF14577 476 - 540 2.9e-14 Sieve element occlusion C-terminus
SEO_C PF14577 549 - 674 5.3e-34 Sieve element occlusion C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000511)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g02370 FvH4_4g02370 FvH4_4g02370 FvH4_4g02430 FvH4_4g02430 FvH4_4g02460 FvH4_4g02460 FvH4_4g02460 FvH4_4g02480 FvH4_4g02480 FvH4_4g02480 FvH4_4g02480 FvH4_6g05920
malus_domestica MD00G1111000.v1.1 MD08G1225800.v1.1 MD13G1205700.v1.1 MD13G1205800.v1.1 MD13G1205900.v1.1 MD13G1206000.v1.1 MD16G1207500.v1.1 MD16G1207600.v1.1
prunus_persica Prupe.1G028400_v2.0.a1 Prupe.1G028500_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028900_v2.0.a1 Prupe.1G029000_v2.0.a1 Prupe.1G029100_v2.0.a1
pyrus_communis pycom08g19680 pycom08g19730 pycom16g17460 pycom16g17500
rosa_chinensis RchiOBHm_Chr4g0389751 RchiOBHm_Chr4g0389771 RchiOBHm_Chr4g0389791 RchiOBHm_Chr4g0389861 RchiOBHm_Chr4g0389881 RchiOBHm_Chr4g0389901 RchiOBHm_Chr4g0412411
rosa_laevigata RLG00000008288 RLG00000009901 RLG00000009945 RLG00000009954 RLG00000009956 RLG00000009957 RLG00000009958 RLG00000019246
rosa_multiflora Rmu_co8275125.1_g000001 Rmu_sc0002083.1_g000003 Rmu_sc0002083.1_g000025 Rmu_sc0002604.1_g000016 Rmu_sc0004336.1_g000031 Rmu_sc0010423.1_g000003
rosa_roxburghii Rroxscaffold_5G00335790 Rroxscaffold_5G00335850 Rroxscaffold_5G00335970 Rroxscaffold_5G00335990
rosa_rugosa Rorug03G0325100 Rorug03G0325300 Rorug03G0325500.1 Rorug03G0326800 Rorug03G0327000 Rorug04G0104400
rosa_samantha Rh4AG029900 Rh4AG030000 Rh4AG030100 Rh4AG030200 Rh4AG030600 Rh4AG172900 Rh4BG023200 Rh4BG023900 Rh4BG024000 Rh4BG024100 Rh4BG172200 Rh4DG023300 Rh4DG023800 Rh4DG023900 Rh4DG024000 Rh4DG151600
rosa_wichuraiana Rw1G002330 Rw1G002340 Rw4G002260 Rw4G002270 Rw4G002280 Rw4G002300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1681
Acc36I ACCTGC 1 cut(s) 1681
AccB1I GGYRCC 1 cut(s) 1968
AccI GTMKAC 2 cut(s) 1772, 1909
AccII CGCG 1 cut(s) 480
AccIII TCCGGA 1 cut(s) 1954
AciI CCGC 1 cut(s) 1754
AclWI GGATC 5 cut(s) 696, 896, 1081, 1469, 1547
AcsI RAATTY 4 cut(s) 455, 807, 1172, 1261
AcuI CTGAAG 1 cut(s) 1205
AdeI CACNNNGTG 1 cut(s) 22
AfaI GTAC 3 cut(s) 235, 291, 1217
AfiI CCNNNNNNNGG 7 cut(s) 72, 595, 1232, 1588, 1690, 1849, 1990
AgeI ACCGGT 1 cut(s) 627
AhlI ACTAGT 1 cut(s) 761
AjnI CCWGG 2 cut(s) 340, 465
AjuI GAANNNNNNNTTGG 2 cut(s) 266, 298
AleI CACNNNNGTG 1 cut(s) 368
AluBI AGCT 9 cut(s) 79, 388, 434, 475, 550, 580, 829, 841, 1723
AluI AGCT 9 cut(s) 79, 388, 434, 475, 550, 580, 829, 841, 1723
Alw21I GWGCWC 2 cut(s) 302, 1455
Alw26I GTCTC 5 cut(s) 869, 1060, 1101, 1804, 1988
Alw44I GTGCAC 2 cut(s) 298, 1451
AlwI GGATC 5 cut(s) 696, 896, 1081, 1469, 1547
Ama87I CYCGRG 2 cut(s) 72, 1219
Aor13HI TCCGGA 1 cut(s) 1954
AoxI GGCC 6 cut(s) 7, 36, 597, 1356, 1639, 1681
ApaLI GTGCAC 2 cut(s) 298, 1451
ApeKI GCWGC 6 cut(s) 431, 472, 550, 1608, 1917, 2016
ApoI RAATTY 4 cut(s) 455, 807, 1172, 1261
AsiGI ACCGGT 1 cut(s) 627
Asp700I GAANNNNTTC 2 cut(s) 899, 1512
AspLEI GCGC 2 cut(s) 472, 561
AspS9I GGNCC 4 cut(s) 1223, 1650, 1840, 2030
AsuC2I CCSGG 1 cut(s) 1935
AsuHPI GGTGA 5 cut(s) 464, 1300, 1433, 1571, 1666
AvaI CYCGRG 2 cut(s) 72, 1219
AvaII GGWCC 4 cut(s) 1223, 1650, 1840, 2030
AxyI CCTNAGG 1 cut(s) 1013
BaeGI GKGCMC 3 cut(s) 302, 1455, 1973
BaeI ACNNNNGTAYC 2 cut(s) 225, 258
BanI GGYRCC 1 cut(s) 1968
BanII GRGCYC 1 cut(s) 1940
BarI GAAGNNNNNNTAC 2 cut(s) 1469, 1501
BauI CACGAG 1 cut(s) 1091
BbsI GAAGAC 1 cut(s) 1626
Bbv12I GWGCWC 2 cut(s) 302, 1455
BbvI GCAGC 6 cut(s) 443, 484, 537, 1620, 1904, 2003
BccI CCATC 4 cut(s) 971, 1453, 1743, 2021
BceAI ACGGC 3 cut(s) 526, 1626, 1905
BcgI CGANNNNNNTGC 2 cut(s) 1899, 1933
BciT130I CCWGG 2 cut(s) 342, 467
BclI TGATCA 1 cut(s) 591
BcnI CCSGG 1 cut(s) 1935
BcoDI GTCTC 5 cut(s) 869, 1060, 1101, 1804, 1988
BcuI ACTAGT 1 cut(s) 761
BfaI CTAG 9 cut(s) 5, 11, 428, 762, 788, 1049, 1583, 1637, 1905
BfmI CTRYAG 1 cut(s) 1377
BfuAI ACCTGC 1 cut(s) 1681
BglII AGATCT 1 cut(s) 108
BisI GCNGC 6 cut(s) 432, 473, 551, 1609, 1918, 2017
BlpI GCTNAGC 2 cut(s) 80, 1760
BlsI GCNGC 6 cut(s) 433, 474, 552, 1610, 1919, 2018
BmcAI AGTACT 1 cut(s) 1217
Bme1390I CCNGG 3 cut(s) 342, 467, 1935
Bme18I GGWCC 4 cut(s) 1223, 1650, 1840, 2030
BmeT110I CYCGRG 2 cut(s) 72, 1219
BmgT120I GGNCC 4 cut(s) 1223, 1650, 1840, 2030
BmiI GGNNCC 6 cut(s) 464, 1224, 1351, 1423, 1953, 1970
BmrFI CCNGG 3 cut(s) 342, 467, 1935
BmsI GCATC 4 cut(s) 315, 425, 630, 1245
BpiI GAAGAC 1 cut(s) 1626
BpmI CTGGAG 2 cut(s) 363, 521
Bpu1102I GCTNAGC 2 cut(s) 80, 1760
BpuEI CTTGAG 3 cut(s) 179, 222, 596
BpuMI CCSGG 1 cut(s) 1935
Bsa29I ATCGAT 1 cut(s) 975
BsaJI CCNNGG 5 cut(s) 30, 684, 1199, 1353, 1627
BsaWI WCCGGW 3 cut(s) 627, 697, 1954
BsaXI ACNNNNNCTCC 2 cut(s) 1844, 1874
Bsc4I CCNNNNNNNGG 7 cut(s) 72, 595, 1232, 1588, 1690, 1849, 1990
Bse118I RCCGGY 1 cut(s) 627
Bse1I ACTGG 3 cut(s) 279, 1653, 2041
Bse21I CCTNAGG 1 cut(s) 1013
Bse3DI GCAATG 3 cut(s) 66, 335, 1154
BseAI TCCGGA 1 cut(s) 1954
BseBI CCWGG 2 cut(s) 342, 467
BseCI ATCGAT 1 cut(s) 975
BseDI CCNNGG 5 cut(s) 30, 684, 1199, 1353, 1627
BseGI GGATG 4 cut(s) 189, 1008, 1445, 1754
BseLI CCNNNNNNNGG 7 cut(s) 72, 595, 1232, 1588, 1690, 1849, 1990
BseMI GCAATG 3 cut(s) 66, 335, 1154
BseMII CTCAG 3 cut(s) 54, 71, 1154
BseNI ACTGG 3 cut(s) 279, 1653, 2041
BseRI GAGGAG 5 cut(s) 131, 275, 524, 881, 1268
BseSI GKGCMC 3 cut(s) 302, 1455, 1973
BseXI GCAGC 6 cut(s) 443, 484, 537, 1620, 1904, 2003
BsgI GTGCAG 3 cut(s) 248, 536, 1232
Bsh1236I CGCG 1 cut(s) 480
Bsh1285I CGRYCG 1 cut(s) 628
BshFI GGCC 6 cut(s) 9, 38, 599, 1358, 1641, 1683
BshNI GGYRCC 1 cut(s) 1968
BshTI ACCGGT 1 cut(s) 627
BshVI ATCGAT 1 cut(s) 975
BsiEI CGRYCG 1 cut(s) 628
BsiHKAI GWGCWC 2 cut(s) 302, 1455
BsiHKCI CYCGRG 2 cut(s) 72, 1219
BsiSI CCGG 4 cut(s) 628, 698, 1934, 1955
BslFI GGGAC 4 cut(s) 509, 650, 951, 1236
BslI CCNNNNNNNGG 7 cut(s) 72, 595, 1232, 1588, 1690, 1849, 1990
BsmAI GTCTC 5 cut(s) 869, 1060, 1101, 1804, 1988
BsmFI GGGAC 4 cut(s) 509, 650, 951, 1236
BsmI GAATGC 1 cut(s) 623
BsnI GGCC 6 cut(s) 9, 38, 599, 1358, 1641, 1683
BsoBI CYCGRG 2 cut(s) 72, 1219
Bsp1286I GDGCHC 4 cut(s) 302, 1455, 1940, 1973
Bsp13I TCCGGA 1 cut(s) 1954
Bsp1720I GCTNAGC 2 cut(s) 80, 1760
Bsp19I CCATGG 1 cut(s) 1627
Bsp68I TCGCGA 1 cut(s) 480
BspACI CCGC 1 cut(s) 1754
BspANI GGCC 6 cut(s) 9, 38, 599, 1358, 1641, 1683
BspCNI CTCAG 3 cut(s) 53, 70, 1155
BspDI ATCGAT 1 cut(s) 975
BspEI TCCGGA 1 cut(s) 1954
BspFNI CGCG 1 cut(s) 480
BspHI TCATGA 1 cut(s) 1885
BspLI GGNNCC 6 cut(s) 464, 1224, 1351, 1423, 1953, 1970
BspMI ACCTGC 1 cut(s) 1681
BspPI GGATC 5 cut(s) 696, 896, 1081, 1469, 1547
BspT107I GGYRCC 1 cut(s) 1968
BsrDI GCAATG 3 cut(s) 66, 335, 1154
BsrFI RCCGGY 1 cut(s) 627
BsrI ACTGG 3 cut(s) 279, 1653, 2041
BssAI RCCGGY 1 cut(s) 627
BssECI CCNNGG 5 cut(s) 30, 684, 1199, 1353, 1627
BssSI CACGAG 1 cut(s) 1091
BssT1I CCWWGG 5 cut(s) 30, 684, 1199, 1353, 1627
Bst2BI CACGAG 1 cut(s) 1091
Bst2UI CCWGG 2 cut(s) 342, 467
Bst4CI ACNGT 8 cut(s) 370, 727, 997, 1210, 1282, 1392, 1777, 1985
BstAPI GCANNNNNTGC 1 cut(s) 437
BstC8I GCNNGC 5 cut(s) 201, 302, 335, 563, 1234
BstDEI CTNAG 9 cut(s) 40, 57, 80, 744, 842, 987, 1013, 1163, 1760
BstDSI CCRYGG 1 cut(s) 1627
BstENI CCTNNNNNAGG 2 cut(s) 593, 1230
BstF5I GGATG 4 cut(s) 189, 1008, 1445, 1754
BstFNI CGCG 1 cut(s) 480
BstHHI GCGC 2 cut(s) 472, 561
BstMAI GTCTC 5 cut(s) 869, 1060, 1101, 1804, 1988
BstMCI CGRYCG 1 cut(s) 628
BstMWI GCNNNNNNNGC 6 cut(s) 334, 437, 469, 547, 556, 1977
BstNI CCWGG 2 cut(s) 342, 467
BstNSI RCATGY 1 cut(s) 248
BstSCI CCNGG 3 cut(s) 340, 465, 1933
BstSFI CTRYAG 1 cut(s) 1377
BstSLI GKGCMC 3 cut(s) 302, 1455, 1973
BstUI CGCG 1 cut(s) 480
BstV1I GCAGC 6 cut(s) 443, 484, 537, 1620, 1904, 2003
BstV2I GAAGAC 1 cut(s) 1626
BstX2I RGATCY 1 cut(s) 108
BstXI CCANNNNNNTGG 1 cut(s) 2027
BstYI RGATCY 1 cut(s) 108
Bsu15I ATCGAT 1 cut(s) 975
Bsu36I CCTNAGG 1 cut(s) 1013
BsuRI GGCC 6 cut(s) 9, 38, 599, 1358, 1641, 1683
BsuTUI ATCGAT 1 cut(s) 975
BtgI CCRYGG 1 cut(s) 1627
BtsCI GGATG 4 cut(s) 189, 1008, 1445, 1754
BtsIMutI CAGTG 6 cut(s) 292, 375, 419, 723, 1660, 1707
BtuMI TCGCGA 1 cut(s) 480
BveI ACCTGC 1 cut(s) 1681
Cac8I GCNNGC 5 cut(s) 201, 302, 335, 563, 1234
CciI TCATGA 1 cut(s) 1885
CfoI GCGC 2 cut(s) 472, 561
Cfr10I RCCGGY 1 cut(s) 627
Cfr13I GGNCC 4 cut(s) 1223, 1650, 1840, 2030
ClaI ATCGAT 1 cut(s) 975
CseI GACGC 1 cut(s) 1585
Csp6I GTAC 3 cut(s) 234, 290, 1216
CspAI ACCGGT 1 cut(s) 627
CviQI GTAC 3 cut(s) 234, 290, 1216
DdeI CTNAG 9 cut(s) 40, 57, 80, 744, 842, 987, 1013, 1163, 1760
DraIII CACNNNGTG 1 cut(s) 22
Eco130I CCWWGG 5 cut(s) 30, 684, 1199, 1353, 1627
Eco147I AGGCCT 2 cut(s) 9, 1358
Eco24I GRGCYC 1 cut(s) 1940
Eco32I GATATC 1 cut(s) 1180
Eco47I GGWCC 4 cut(s) 1223, 1650, 1840, 2030
Eco57I CTGAAG 1 cut(s) 1205
Eco81I CCTNAGG 1 cut(s) 1013
Eco88I CYCGRG 2 cut(s) 72, 1219
EcoNI CCTNNNNNAGG 2 cut(s) 593, 1230
EcoO109I RGGNCCY 1 cut(s) 1223
EcoRI GAATTC 1 cut(s) 455
EcoRII CCWGG 2 cut(s) 340, 465
EcoRV GATATC 1 cut(s) 1180
EcoT14I CCWWGG 5 cut(s) 30, 684, 1199, 1353, 1627
EcoT22I ATGCAT 1 cut(s) 623
EcoT38I GRGCYC 1 cut(s) 1940
ErhI CCWWGG 5 cut(s) 30, 684, 1199, 1353, 1627
FalI AAGNNNNNCTT 2 cut(s) 1466, 1498
FaqI GGGAC 4 cut(s) 509, 650, 951, 1236
FbaI TGATCA 1 cut(s) 591
FblI GTMKAC 2 cut(s) 1772, 1909
Fnu4HI GCNGC 6 cut(s) 432, 473, 551, 1609, 1918, 2017
FokI GGATG 4 cut(s) 176, 995, 1432, 1761
FriOI GRGCYC 1 cut(s) 1940
Fsp4HI GCNGC 6 cut(s) 432, 473, 551, 1609, 1918, 2017
FspBI CTAG 9 cut(s) 5, 11, 428, 762, 788, 1049, 1583, 1637, 1905
GlaI GCGC 2 cut(s) 471, 560
GluI GCNGC 6 cut(s) 432, 473, 551, 1609, 1918, 2017
GsuI CTGGAG 2 cut(s) 363, 521
HaeIII GGCC 6 cut(s) 9, 38, 599, 1358, 1641, 1683
HapII CCGG 4 cut(s) 628, 698, 1934, 1955
HgaI GACGC 1 cut(s) 1585
HhaI GCGC 2 cut(s) 472, 561
Hin6I GCGC 2 cut(s) 470, 559
HinP1I GCGC 2 cut(s) 470, 559
HincII GTYRAC 2 cut(s) 1773, 1910
HindII GTYRAC 2 cut(s) 1773, 1910
HindIII AAGCTT 1 cut(s) 1721
HpaII CCGG 4 cut(s) 628, 698, 1934, 1955
HphI GGTGA 5 cut(s) 464, 1300, 1433, 1571, 1666
Hpy166II GTNNAC 9 cut(s) 49, 139, 250, 290, 300, 1206, 1453, 1773, 1910
Hpy188I TCNGA 6 cut(s) 60, 487, 547, 905, 1060, 1601
Hpy8I GTNNAC 9 cut(s) 49, 139, 250, 290, 300, 1206, 1453, 1773, 1910
Hpy99I CGWCG 1 cut(s) 1601
HpyAV CCTTC 5 cut(s) 879, 1369, 1427, 1804, 1831
HpyCH4III ACNGT 8 cut(s) 370, 727, 997, 1210, 1282, 1392, 1777, 1985
HpyF10VI GCNNNNNNNGC 6 cut(s) 334, 437, 469, 547, 556, 1977
HpyF3I CTNAG 9 cut(s) 40, 57, 80, 744, 842, 987, 1013, 1163, 1760
HspAI GCGC 2 cut(s) 470, 559
Kpn2I TCCGGA 1 cut(s) 1954
Ksp22I TGATCA 1 cut(s) 591
LmnI GCTCC 5 cut(s) 76, 468, 1144, 1349, 1427
Lsp1109I GCAGC 6 cut(s) 443, 484, 537, 1620, 1904, 2003
LweI GCATC 4 cut(s) 315, 425, 630, 1245
MaeI CTAG 9 cut(s) 5, 11, 428, 762, 788, 1049, 1583, 1637, 1905
MaeIII GTNAC 3 cut(s) 1392, 1783, 2006
MboII GAAGA 7 cut(s) 1198, 1394, 1397, 1474, 1477, 1552, 1631
MfeI CAATTG 2 cut(s) 504, 1137
MflI RGATCY 1 cut(s) 108
MhlI GDGCHC 4 cut(s) 302, 1455, 1940, 1973
MlyI GAGTC 3 cut(s) 531, 1706, 1967
MmeI TCCRAC 2 cut(s) 1344, 1579
Mph1103I ATGCAT 1 cut(s) 623
MroI TCCGGA 1 cut(s) 1954
MroXI GAANNNNTTC 2 cut(s) 899, 1512
MseI TTAA 3 cut(s) 582, 999, 2047
MslI CAYNNNNRTG 4 cut(s) 144, 368, 1401, 2025
MspI CCGG 4 cut(s) 628, 698, 1934, 1955
MspR9I CCNGG 3 cut(s) 342, 467, 1935
MunI CAATTG 2 cut(s) 504, 1137
Mva1269I GAATGC 1 cut(s) 623
MvaI CCWGG 2 cut(s) 342, 467
MvnI CGCG 1 cut(s) 480
MwoI GCNNNNNNNGC 6 cut(s) 334, 437, 469, 547, 556, 1977
NciI CCSGG 1 cut(s) 1935
NcoI CCATGG 1 cut(s) 1627
NlaIV GGNNCC 6 cut(s) 464, 1224, 1351, 1423, 1953, 1970
NmuCI GTSAC 2 cut(s) 1392, 1783
NruI TCGCGA 1 cut(s) 480
NsiI ATGCAT 1 cut(s) 623
NspI RCATGY 1 cut(s) 248
OliI CACNNNNGTG 1 cut(s) 368
PagI TCATGA 1 cut(s) 1885
PaqCI CACCTGC 1 cut(s) 1681
PceI AGGCCT 2 cut(s) 9, 1358
PcsI WCGNNNNNNNCGW 1 cut(s) 621
PctI GAATGC 1 cut(s) 623
PdmI GAANNNNTTC 2 cut(s) 899, 1512
PflFI GACNNNGTC 1 cut(s) 1844
PfoI TCCNGGA 1 cut(s) 340
PinAI ACCGGT 1 cut(s) 627
PkrI GCNGC 6 cut(s) 433, 474, 552, 1610, 1919, 2018
PleI GAGTC 3 cut(s) 530, 1705, 1966
PpsI GAGTC 3 cut(s) 530, 1705, 1966
PpuMI RGGWCCY 1 cut(s) 1223
Psp5II RGGWCCY 1 cut(s) 1223
Psp6I CCWGG 2 cut(s) 340, 465
PspGI CCWGG 2 cut(s) 340, 465
PspN4I GGNNCC 6 cut(s) 464, 1224, 1351, 1423, 1953, 1970
PspPI GGNCC 4 cut(s) 1223, 1650, 1840, 2030
PspPPI RGGWCCY 1 cut(s) 1223
PsuI RGATCY 1 cut(s) 108
PsyI GACNNNGTC 1 cut(s) 1844
RruI TCGCGA 1 cut(s) 480
RsaI GTAC 3 cut(s) 235, 291, 1217
RsaNI GTAC 3 cut(s) 234, 290, 1216
RseI CAYNNNNRTG 4 cut(s) 144, 368, 1401, 2025
SalI GTCGAC 2 cut(s) 1771, 1908
SaqAI TTAA 3 cut(s) 582, 999, 2047
SatI GCNGC 6 cut(s) 432, 473, 551, 1609, 1918, 2017
Sau96I GGNCC 4 cut(s) 1223, 1650, 1840, 2030
ScaI AGTACT 1 cut(s) 1217
SchI GAGTC 3 cut(s) 531, 1706, 1967
ScrFI CCNGG 3 cut(s) 342, 467, 1935
SduI GDGCHC 4 cut(s) 302, 1455, 1940, 1973
SfaNI GCATC 4 cut(s) 315, 425, 630, 1245
SfcI CTRYAG 1 cut(s) 1377
SinI GGWCC 4 cut(s) 1223, 1650, 1840, 2030
SmiMI CAYNNNNRTG 4 cut(s) 144, 368, 1401, 2025
SmlI CTYRAG 3 cut(s) 194, 237, 575
SmoI CTYRAG 3 cut(s) 194, 237, 575
SpeI ACTAGT 1 cut(s) 761
SseBI AGGCCT 2 cut(s) 9, 1358
SsiI CCGC 1 cut(s) 1754
SspMI CTAG 9 cut(s) 5, 11, 428, 762, 788, 1049, 1583, 1637, 1905
StuI AGGCCT 2 cut(s) 9, 1358
StyD4I CCNGG 3 cut(s) 340, 465, 1933
StyI CCWWGG 5 cut(s) 30, 684, 1199, 1353, 1627
TaaI ACNGT 8 cut(s) 370, 727, 997, 1210, 1282, 1392, 1777, 1985
TaqI TCGA 9 cut(s) 615, 624, 677, 975, 1005, 1073, 1772, 1909, 1961
TatI WGTACW 1 cut(s) 1215
Tru1I TTAA 3 cut(s) 582, 999, 2047
Tru9I TTAA 3 cut(s) 582, 999, 2047
TscAI CASTG 6 cut(s) 292, 375, 426, 730, 1660, 1707
TseFI GTSAC 2 cut(s) 1392, 1783
TseI GCWGC 6 cut(s) 431, 472, 550, 1608, 1917, 2016
Tsp45I GTSAC 2 cut(s) 1392, 1783
TspDTI ATGAA 7 cut(s) 162, 363, 390, 1027, 1087, 1234, 1307
TspGWI ACGGA 3 cut(s) 1414, 1438, 1959
TspRI CASTG 6 cut(s) 292, 375, 426, 730, 1660, 1707
Tth111I GACNNNGTC 1 cut(s) 1844
VneI GTGCAC 2 cut(s) 298, 1451
VpaK11BI GGWCC 4 cut(s) 1223, 1650, 1840, 2030
XagI CCTNNNNNAGG 2 cut(s) 593, 1230
XapI RAATTY 4 cut(s) 455, 807, 1172, 1261
XceI RCATGY 1 cut(s) 248
XmiI GTMKAC 2 cut(s) 1772, 1909
XmnI GAANNNNTTC 2 cut(s) 899, 1512
XspI CTAG 9 cut(s) 5, 11, 428, 762, 788, 1049, 1583, 1637, 1905
ZrmI AGTACT 1 cut(s) 1217
Zsp2I ATGCAT 1 cut(s) 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.