Rh4AG172900

Sieve element occlusion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000511
Physical Location & Seq
Forward (+)
43402401 .. 43405392
2992 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG172900.1

Sequence Viewer

Length: 2046 bp
ATGCTAGGCCTAGCATTCAATGTGGCTACCAAGGTAGCCGCAGCAGTCCACCCTACTCACAAAACCATTGATGAGGAGTTTAGCCTGTTTGCCATGTCTGACAAGCAGATCTTGCAGGAGATTTACACGACTCATGTCCATGCTGATGAGACCTTGGATGATGATTCTCTCTTCATCATTGTTGAGAATATCCTCAAGCATGCAACCCAAAATGTTGATGAAATAGTGCAGGGTATCCAAGTGCATGTGGACAACATAGAGGAGAAAAACCCCAAAGCCAGCTTCAGCGTACCATTGTGCACGCTAAAGGGAATCTCCTGCGAGATGCAATGCAAGCCTCCTGGTGACGAAATCGCCCACAACACAACAGTGGCAATACTCAACAAGCTCTCAAAGTATTCATGGGAAGCAAAGGCATCACTGACCCTAGCTGCTTTTGCTATGGAATATGGTGAATTCTGGCTCCTTGCGCAGCTTCGCAAATCAGACCACCTTGCCAAATCAATTGCAATCCTCAAACGAGTCCCTGTTCTCCTCAAGCCAACGGAGCTGCAAAAGCGCAGGCAATCAATTCTTCAGCTTAACAATCTGATCAAGTCCACACTACAAGTGATCGAATGCATCGGCCAGTTCAATAAGTATTCCCGTTATCTCCCAAAAGAATTCCCCGGTTTGTCGATAGCATCGGAACATATTCCGGTTGATGTCTATTGGGCTATCATCACTGTTGTTGCTTGTGCCAGTAAGATCACTATACTAACTAGTGATGAGGACAAGGAAATTGATCTAGCCCCCTATTCTCAAAAAATCCACTATGTCCTTAACAAGCTAACGATTCAGCTGAGAGGTTGCAAAACATATATTGAGGAGGAAGAGACTTACATAAGGATCAAGAAATTGTTCCAAACGCCTACTGAAATTATGGAGGTTTTCAAGGGATTAATATTTACAAAGGATAATGTCCAGCCACTCGTCGATGGTTCAACTAAGCAAACGGTTAACATCGACATCCTAAGGAGGAAGAATGTGCTATTGTTCATTTCCAGTCTAGACATTACGGATGACGACATTTCGATTCTAAAACCGATCCACGAGTTCACGAAGAAAGACAATCAGTATAACATTGTCTGGATACCAATTGTGGAGCAATGGACTGATGACCTGAGAAAGAAGTTCGATATCCTGAAGAACAAGATACCGTGGTTTACGGTGCAGTACCCTGGACCTATAGCAGGCATCAAGTACATCAAGAAGGACTGGAACTTCAAGGGTAAGCCTACGATGGTTGTGATGAACCCACATGGAAAGGTCGAACACCCTAACGCTGTCCACATGGTTCGGGTATGGGGAGTAAAGGCCTTCCCTTTCACAGAGACAAGAGAAGCAGAACTGTCACATTCTGATGGAGAACAATGGGTTTCCTCCGTTGTTAACGGAATTCACCCATCTCTGCCCACTTGGATCAAAGAAAATAAGTACATCTTCTTCTATGGCGGTAAAGACAACGTATGGATTCAGAATTTTAAAAATAAAGCAACTGCCCTTGCAAAAGATCCAATCTTCAAGGAAGCAAAGATAGAGTTGGTTTGCGTTGGAAAAGACAGCAAAGGAGAAGATGACCGTGGCGTTCTAGGCCGCTTCTGGACCGGTGTAGAAAGCCTGTTCTTAACCACAGGCCAATGGCAGGTCGACTCGGTCAGTGAAGAAATCCAGAAGCTTCTCTCTTACAAAAACGAAAGTGGATGGGCAGTGCTTTGCAAAGGGTCGACTGTGTTGGTCACGGCTCATGGTGTCTCGGTGTTGAAGGTGGTAGAGGACTTTGAGAAATGGAAGGACCCTGTCAAGGAGAACGGGTTTGAGTTTTGTTTCACAACCTACTATGGGACGATTATTCAAGGTAGCCGTCAACCTTGCTGCCGTGTGGACATTCCGGGGTCTAACGGAAAGGTTCCAGATTCGATGAAGTGCCCTGATTGCCACCGTAGTATGGAGACTATTATCAGTTACAAGTGCTGCCACACTGATGGTCCTATTGCACATCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SEO_C SEO_N

Protein Analysis

681

Amino Acids

77.34

Weight (kDa)

6.73

Isoelectric Point (pI)

35.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SEO_N PF14576 33 - 304 3.3e-82 Sieve element occlusion N-terminus
SEO_C PF14577 474 - 541 5.1e-12 Sieve element occlusion C-terminus
SEO_C PF14577 547 - 673 2.6e-30 Sieve element occlusion C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000511)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g02370 FvH4_4g02370 FvH4_4g02370 FvH4_4g02430 FvH4_4g02430 FvH4_4g02460 FvH4_4g02460 FvH4_4g02460 FvH4_4g02480 FvH4_4g02480 FvH4_4g02480 FvH4_4g02480 FvH4_6g05920
malus_domestica MD00G1111000.v1.1 MD08G1225800.v1.1 MD13G1205700.v1.1 MD13G1205800.v1.1 MD13G1205900.v1.1 MD13G1206000.v1.1 MD16G1207500.v1.1 MD16G1207600.v1.1
prunus_persica Prupe.1G028400_v2.0.a1 Prupe.1G028500_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028900_v2.0.a1 Prupe.1G029000_v2.0.a1 Prupe.1G029100_v2.0.a1
pyrus_communis pycom08g19680 pycom08g19730 pycom16g17460 pycom16g17500
rosa_chinensis RchiOBHm_Chr4g0389751 RchiOBHm_Chr4g0389771 RchiOBHm_Chr4g0389791 RchiOBHm_Chr4g0389861 RchiOBHm_Chr4g0389881 RchiOBHm_Chr4g0389901 RchiOBHm_Chr4g0412411
rosa_laevigata RLG00000008288 RLG00000009901 RLG00000009945 RLG00000009954 RLG00000009956 RLG00000009957 RLG00000009958 RLG00000019246
rosa_multiflora Rmu_co8275125.1_g000001 Rmu_sc0002083.1_g000003 Rmu_sc0002083.1_g000025 Rmu_sc0002604.1_g000016 Rmu_sc0004336.1_g000031 Rmu_sc0010423.1_g000003
rosa_roxburghii Rroxscaffold_5G00335790 Rroxscaffold_5G00335850 Rroxscaffold_5G00335970 Rroxscaffold_5G00335990
rosa_rugosa Rorug03G0325100 Rorug03G0325300 Rorug03G0325500.1 Rorug03G0326800 Rorug03G0327000 Rorug04G0104400
rosa_samantha Rh4AG029900 Rh4AG030000 Rh4AG030100 Rh4AG030200 Rh4AG030600 Rh4AG172900 Rh4BG023200 Rh4BG023900 Rh4BG024000 Rh4BG024100 Rh4BG172200 Rh4DG023300 Rh4DG023800 Rh4DG023900 Rh4DG024000 Rh4DG151600
rosa_wichuraiana Rw1G002330 Rw1G002340 Rw4G002260 Rw4G002270 Rw4G002280 Rw4G002300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 471
Acc36I ACCTGC 1 cut(s) 1675
AccI GTMKAC 2 cut(s) 1689, 1766
AciI CCGC 3 cut(s) 39, 1494, 1636
AclWI GGATC 4 cut(s) 896, 1081, 1469, 1547
AcoI YGGCCR 1 cut(s) 625
AcsI RAATTY 4 cut(s) 455, 662, 1437, 1519
AcuI CTGAAG 3 cut(s) 268, 560, 1205
AfaI GTAC 4 cut(s) 291, 1217, 1244, 1478
AfiI CCNNNNNNNGG 5 cut(s) 1232, 1684, 1843, 1881, 1987
AgeI ACCGGT 1 cut(s) 1646
AgsI TTSAA 8 cut(s) 19, 634, 934, 984, 1267, 1564, 1804, 1895
AhlI ACTAGT 1 cut(s) 761
AjnI CCWGG 2 cut(s) 340, 1219
AjuI GAANNNNNNNTTGG 2 cut(s) 266, 298
AleI CACNNNNGTG 1 cut(s) 368
AluBI AGCT 9 cut(s) 282, 388, 431, 475, 550, 580, 829, 841, 1717
AluI AGCT 9 cut(s) 282, 388, 431, 475, 550, 580, 829, 841, 1717
Alw21I GWGCWC 1 cut(s) 302
Alw26I GTCTC 5 cut(s) 143, 869, 1367, 1798, 1985
Alw44I GTGCAC 1 cut(s) 298
AlwI GGATC 4 cut(s) 896, 1081, 1469, 1547
AoxI GGCC 5 cut(s) 7, 625, 1356, 1633, 1675
ApaLI GTGCAC 1 cut(s) 298
ApeKI GCWGC 6 cut(s) 41, 431, 472, 550, 1914, 2013
ApoI RAATTY 4 cut(s) 455, 662, 1437, 1519
AseI ATTAAT 1 cut(s) 941
AsiGI ACCGGT 1 cut(s) 1646
Asp700I GAANNNNTTC 2 cut(s) 693, 899
AspLEI GCGC 2 cut(s) 472, 561
AspS9I GGNCC 4 cut(s) 1223, 1644, 1834, 2027
AsuC2I CCSGG 2 cut(s) 669, 1932
AsuHPI GGTGA 3 cut(s) 356, 464, 1433
AvaII GGWCC 4 cut(s) 1223, 1644, 1834, 2027
AxyI CCTNAGG 1 cut(s) 1013
BaeGI GKGCMC 2 cut(s) 302, 1970
BaeI ACNNNNGTAYC 2 cut(s) 1187, 1220
BarI GAAGNNNNNNTAC 2 cut(s) 1469, 1501
BauI CACGAG 1 cut(s) 1091
Bbv12I GWGCWC 1 cut(s) 302
BbvI GCAGC 6 cut(s) 53, 418, 484, 537, 1901, 2000
BccI CCATC 6 cut(s) 971, 1276, 1397, 1453, 1737, 2018
BceAI ACGGC 3 cut(s) 1797, 1887, 1902
BciT130I CCWGG 2 cut(s) 342, 1221
BciVI GTATCC 2 cut(s) 245, 1125
BclI TGATCA 1 cut(s) 591
BcnI CCSGG 2 cut(s) 669, 1932
BcoDI GTCTC 5 cut(s) 143, 869, 1367, 1798, 1985
BcuI ACTAGT 1 cut(s) 761
BfaI CTAG 7 cut(s) 5, 11, 428, 762, 788, 1049, 1631
BfmI CTRYAG 1 cut(s) 1227
BfuAI ACCTGC 1 cut(s) 1675
BfuI GTATCC 2 cut(s) 245, 1125
BglII AGATCT 1 cut(s) 108
BisI GCNGC 8 cut(s) 39, 42, 432, 473, 551, 1636, 1915, 2014
BlsI GCNGC 8 cut(s) 40, 43, 433, 474, 552, 1637, 1916, 2015
Bme1390I CCNGG 4 cut(s) 342, 669, 1221, 1932
Bme18I GGWCC 4 cut(s) 1223, 1644, 1834, 2027
BmgT120I GGNCC 4 cut(s) 1223, 1644, 1834, 2027
BmiI GGNNCC 3 cut(s) 464, 1836, 1950
BmrFI CCNGG 4 cut(s) 342, 669, 1221, 1932
BmsI GCATC 5 cut(s) 315, 425, 630, 692, 1245
BoxI GACNNNNGTC 1 cut(s) 134
BpuEI CTTGAG 2 cut(s) 179, 521
BpuMI CCSGG 2 cut(s) 669, 1932
BsaI GGTCTC 1 cut(s) 143
BsaJI CCNNGG 7 cut(s) 30, 153, 667, 1199, 1219, 1621, 1931
BsaWI WCCGGW 2 cut(s) 697, 1646
BsaXI ACNNNNNCTCC 2 cut(s) 1838, 1868
Bsc4I CCNNNNNNNGG 5 cut(s) 1232, 1684, 1843, 1881, 1987
Bse118I RCCGGY 1 cut(s) 1646
Bse1I ACTGG 4 cut(s) 628, 741, 1044, 1262
Bse21I CCTNAGG 1 cut(s) 1013
Bse3DI GCAATG 2 cut(s) 335, 1154
BseBI CCWGG 2 cut(s) 342, 1221
BseDI CCNNGG 7 cut(s) 30, 153, 667, 1199, 1219, 1621, 1931
BseGI GGATG 4 cut(s) 163, 1008, 1066, 1748
BseLI CCNNNNNNNGG 5 cut(s) 1232, 1684, 1843, 1881, 1987
BseMI GCAATG 2 cut(s) 335, 1154
BseMII CTCAG 2 cut(s) 833, 1154
BseNI ACTGG 4 cut(s) 628, 741, 1044, 1262
BseRI GAGGAG 4 cut(s) 89, 275, 524, 881
BseSI GKGCMC 2 cut(s) 302, 1970
BseXI GCAGC 6 cut(s) 53, 418, 484, 537, 1901, 2000
BsgI GTGCAG 2 cut(s) 248, 1232
BshFI GGCC 5 cut(s) 9, 627, 1358, 1635, 1677
BshTI ACCGGT 1 cut(s) 1646
BsiHKAI GWGCWC 1 cut(s) 302
BsiSI CCGG 4 cut(s) 669, 698, 1647, 1931
BslFI GGGAC 2 cut(s) 509, 1897
BslI CCNNNNNNNGG 5 cut(s) 1232, 1684, 1843, 1881, 1987
BsmAI GTCTC 5 cut(s) 143, 869, 1367, 1798, 1985
BsmFI GGGAC 2 cut(s) 509, 1897
BsmI GAATGC 2 cut(s) 14, 623
BsnI GGCC 5 cut(s) 9, 627, 1358, 1635, 1677
Bso31I GGTCTC 1 cut(s) 143
Bsp1286I GDGCHC 2 cut(s) 302, 1970
Bsp143I GATC 9 cut(s) 108, 591, 612, 747, 784, 888, 1086, 1461, 1552
BspACI CCGC 3 cut(s) 39, 1494, 1636
BspANI GGCC 5 cut(s) 9, 627, 1358, 1635, 1677
BspCNI CTCAG 2 cut(s) 834, 1155
BspLI GGNNCC 3 cut(s) 464, 1836, 1950
BspMI ACCTGC 1 cut(s) 1675
BspPI GGATC 4 cut(s) 896, 1081, 1469, 1547
BspTNI GGTCTC 1 cut(s) 143
BsrDI GCAATG 2 cut(s) 335, 1154
BsrFI RCCGGY 1 cut(s) 1646
BsrI ACTGG 4 cut(s) 628, 741, 1044, 1262
BssAI RCCGGY 1 cut(s) 1646
BssECI CCNNGG 7 cut(s) 30, 153, 667, 1199, 1219, 1621, 1931
BssMI GATC 9 cut(s) 108, 591, 612, 747, 784, 888, 1086, 1461, 1552
BssSI CACGAG 1 cut(s) 1091
BssT1I CCWWGG 2 cut(s) 30, 153
Bst2BI CACGAG 1 cut(s) 1091
Bst2UI CCWGG 2 cut(s) 342, 1221
Bst4CI ACNGT 9 cut(s) 370, 727, 997, 1200, 1210, 1392, 1622, 1771, 1982
Bst6I CTCTTC 2 cut(s) 176, 867
BstAPI GCANNNNNTGC 1 cut(s) 112
BstC8I GCNNGC 6 cut(s) 201, 280, 302, 335, 563, 1234
BstDEI CTNAG 4 cut(s) 842, 987, 1013, 1163
BstDSI CCRYGG 2 cut(s) 1199, 1621
BstENI CCTNNNNNAGG 1 cut(s) 1230
BstF5I GGATG 4 cut(s) 163, 1008, 1066, 1748
BstHHI GCGC 2 cut(s) 472, 561
BstKTI GATC 9 cut(s) 111, 594, 615, 750, 787, 891, 1089, 1464, 1555
BstMAI GTCTC 5 cut(s) 143, 869, 1367, 1798, 1985
BstMBI GATC 9 cut(s) 108, 591, 612, 747, 784, 888, 1086, 1461, 1552
BstMWI GCNNNNNNNGC 8 cut(s) 112, 334, 437, 469, 547, 556, 1632, 1974
BstNI CCWGG 2 cut(s) 342, 1221
BstNSI RCATGY 2 cut(s) 203, 248
BstPAI GACNNNNGTC 1 cut(s) 134
BstSCI CCNGG 4 cut(s) 340, 667, 1219, 1930
BstSFI CTRYAG 1 cut(s) 1227
BstSLI GKGCMC 2 cut(s) 302, 1970
BstV1I GCAGC 6 cut(s) 53, 418, 484, 537, 1901, 2000
BstX2I RGATCY 2 cut(s) 108, 1552
BstXI CCANNNNNNTGG 1 cut(s) 2024
BstYI RGATCY 2 cut(s) 108, 1552
Bsu36I CCTNAGG 1 cut(s) 1013
BsuI GTATCC 2 cut(s) 245, 1125
BsuRI GGCC 5 cut(s) 9, 627, 1358, 1635, 1677
BtgI CCRYGG 2 cut(s) 1199, 1621
BtsCI GGATG 4 cut(s) 163, 1008, 1066, 1748
BtsI GCAGTG 1 cut(s) 1755
BtsIMutI CAGTG 6 cut(s) 375, 419, 723, 1705, 1755, 2019
BveI ACCTGC 1 cut(s) 1675
Cac8I GCNNGC 6 cut(s) 201, 280, 302, 335, 563, 1234
CfoI GCGC 2 cut(s) 472, 561
Cfr10I RCCGGY 1 cut(s) 1646
Cfr13I GGNCC 4 cut(s) 1223, 1644, 1834, 2027
Csp6I GTAC 4 cut(s) 290, 1216, 1243, 1477
CspAI ACCGGT 1 cut(s) 1646
CviAII CATG 9 cut(s) 94, 134, 140, 200, 245, 402, 1301, 1333, 1787
CviQI GTAC 4 cut(s) 290, 1216, 1243, 1477
DdeI CTNAG 4 cut(s) 842, 987, 1013, 1163
DpnI GATC 9 cut(s) 110, 593, 614, 749, 786, 890, 1088, 1463, 1554
DpnII GATC 9 cut(s) 108, 591, 612, 747, 784, 888, 1086, 1461, 1552
DraI TTTAAA 1 cut(s) 1525
EaeI YGGCCR 1 cut(s) 625
Eam1104I CTCTTC 2 cut(s) 176, 867
EarI CTCTTC 2 cut(s) 176, 867
Eco130I CCWWGG 2 cut(s) 30, 153
Eco147I AGGCCT 2 cut(s) 9, 1358
Eco31I GGTCTC 1 cut(s) 143
Eco32I GATATC 1 cut(s) 1180
Eco47I GGWCC 4 cut(s) 1223, 1644, 1834, 2027
Eco57I CTGAAG 3 cut(s) 268, 560, 1205
Eco81I CCTNAGG 1 cut(s) 1013
EcoNI CCTNNNNNAGG 1 cut(s) 1230
EcoO109I RGGNCCY 1 cut(s) 1834
EcoRI GAATTC 3 cut(s) 455, 662, 1437
EcoRII CCWGG 2 cut(s) 340, 1219
EcoRV GATATC 1 cut(s) 1180
EcoT14I CCWWGG 2 cut(s) 30, 153
EcoT22I ATGCAT 1 cut(s) 623
ErhI CCWWGG 2 cut(s) 30, 153
FaeI CATG 9 cut(s) 97, 137, 143, 203, 248, 405, 1304, 1336, 1790
FalI AAGNNNNNCTT 4 cut(s) 95, 127, 1466, 1498
FaqI GGGAC 2 cut(s) 509, 1897
FatI CATG 9 cut(s) 93, 133, 139, 199, 244, 401, 1300, 1332, 1786
FbaI TGATCA 1 cut(s) 591
FblI GTMKAC 2 cut(s) 1689, 1766
Fnu4HI GCNGC 8 cut(s) 39, 42, 432, 473, 551, 1636, 1915, 2014
FokI GGATG 4 cut(s) 170, 995, 1073, 1755
Fsp4HI GCNGC 8 cut(s) 39, 42, 432, 473, 551, 1636, 1915, 2014
FspBI CTAG 7 cut(s) 5, 11, 428, 762, 788, 1049, 1631
FspI TGCGCA 1 cut(s) 471
GlaI GCGC 2 cut(s) 471, 560
GluI GCNGC 8 cut(s) 39, 42, 432, 473, 551, 1636, 1915, 2014
HaeIII GGCC 5 cut(s) 9, 627, 1358, 1635, 1677
HapII CCGG 4 cut(s) 669, 698, 1647, 1931
HhaI GCGC 2 cut(s) 472, 561
Hin1II CATG 9 cut(s) 97, 137, 143, 203, 248, 405, 1304, 1336, 1790
Hin6I GCGC 2 cut(s) 470, 559
HinP1I GCGC 2 cut(s) 470, 559
HincII GTYRAC 5 cut(s) 1000, 1432, 1690, 1767, 1907
HindII GTYRAC 5 cut(s) 1000, 1432, 1690, 1767, 1907
HindIII AAGCTT 1 cut(s) 1715
HinfI GANTC 9 cut(s) 130, 164, 312, 522, 835, 1075, 1513, 1691, 1955
HpaI GTTAAC 2 cut(s) 1000, 1432
HpaII CCGG 4 cut(s) 669, 698, 1647, 1931
HphI GGTGA 3 cut(s) 356, 464, 1433
Hpy188I TCNGA 6 cut(s) 100, 487, 591, 688, 1402, 1518
Hpy188III TCNNGA 9 cut(s) 892, 1049, 1099, 1129, 1183, 1249, 1642, 1711, 1952
Hpy99I CGWCG 1 cut(s) 977
HpyAV CCTTC 4 cut(s) 1246, 1369, 1798, 1825
HpyCH4III ACNGT 9 cut(s) 370, 727, 997, 1200, 1210, 1392, 1622, 1771, 1982
HpyCH4IV ACGT 1 cut(s) 1506
HpyF10VI GCNNNNNNNGC 8 cut(s) 112, 334, 437, 469, 547, 556, 1632, 1974
HpyF3I CTNAG 4 cut(s) 842, 987, 1013, 1163
HpySE526I ACGT 1 cut(s) 1506
Hsp92II CATG 9 cut(s) 97, 137, 143, 203, 248, 405, 1304, 1336, 1790
HspAI GCGC 2 cut(s) 470, 559
Ksp22I TGATCA 1 cut(s) 591
KspAI GTTAAC 2 cut(s) 1000, 1432
Kzo9I GATC 9 cut(s) 108, 591, 612, 747, 784, 888, 1086, 1461, 1552
LmnI GCTCC 3 cut(s) 468, 547, 1144
Lsp1109I GCAGC 6 cut(s) 53, 418, 484, 537, 1901, 2000
LweI GCATC 5 cut(s) 315, 425, 630, 692, 1245
MaeI CTAG 7 cut(s) 5, 11, 428, 762, 788, 1049, 1631
MaeII ACGT 1 cut(s) 1506
MaeIII GTNAC 4 cut(s) 344, 1392, 1777, 2003
MalI GATC 9 cut(s) 110, 593, 614, 749, 786, 890, 1088, 1463, 1554
MboI GATC 9 cut(s) 108, 591, 612, 747, 784, 888, 1086, 1461, 1552
MfeI CAATTG 2 cut(s) 504, 1137
MflI RGATCY 2 cut(s) 108, 1552
MhlI GDGCHC 2 cut(s) 302, 1970
MlyI GAGTC 3 cut(s) 124, 531, 1685
MmeI TCCRAC 1 cut(s) 1573
Mph1103I ATGCAT 1 cut(s) 623
MroXI GAANNNNTTC 2 cut(s) 693, 899
MseI TTAA 8 cut(s) 582, 822, 941, 999, 1431, 1524, 1667, 2044
MslI CAYNNNNRTG 5 cut(s) 138, 144, 368, 1401, 2022
MspA1I CMGCKG 1 cut(s) 841
MspI CCGG 4 cut(s) 669, 698, 1647, 1931
MspR9I CCNGG 4 cut(s) 342, 669, 1221, 1932
MunI CAATTG 2 cut(s) 504, 1137
Mva1269I GAATGC 2 cut(s) 14, 623
MvaI CCWGG 2 cut(s) 342, 1221
MwoI GCNNNNNNNGC 8 cut(s) 112, 334, 437, 469, 547, 556, 1632, 1974
NciI CCSGG 2 cut(s) 669, 1932
NdeII GATC 9 cut(s) 108, 591, 612, 747, 784, 888, 1086, 1461, 1552
NlaIII CATG 9 cut(s) 97, 137, 143, 203, 248, 405, 1304, 1336, 1790
NlaIV GGNNCC 3 cut(s) 464, 1836, 1950
NmuCI GTSAC 3 cut(s) 344, 1392, 1777
NsbI TGCGCA 1 cut(s) 471
NsiI ATGCAT 1 cut(s) 623
NspI RCATGY 2 cut(s) 203, 248
OliI CACNNNNGTG 1 cut(s) 368
PaeI GCATGC 1 cut(s) 203
PceI AGGCCT 2 cut(s) 9, 1358
PctI GAATGC 2 cut(s) 14, 623
PdmI GAANNNNTTC 2 cut(s) 693, 899
PfeI GAWTC 6 cut(s) 164, 312, 835, 1075, 1513, 1955
PflFI GACNNNGTC 2 cut(s) 1694, 1838
PinAI ACCGGT 1 cut(s) 1646
PkrI GCNGC 8 cut(s) 40, 43, 433, 474, 552, 1637, 1916, 2015
PleI GAGTC 3 cut(s) 124, 530, 1685
PpsI GAGTC 3 cut(s) 124, 530, 1685
PpuMI RGGWCCY 1 cut(s) 1834
PshAI GACNNNNGTC 1 cut(s) 134
PshBI ATTAAT 1 cut(s) 941
Psp5II RGGWCCY 1 cut(s) 1834
Psp6I CCWGG 2 cut(s) 340, 1219
PspGI CCWGG 2 cut(s) 340, 1219
PspN4I GGNNCC 3 cut(s) 464, 1836, 1950
PspPI GGNCC 4 cut(s) 1223, 1644, 1834, 2027
PspPPI RGGWCCY 1 cut(s) 1834
PsuI RGATCY 2 cut(s) 108, 1552
PsyI GACNNNGTC 2 cut(s) 1694, 1838
PvuII CAGCTG 1 cut(s) 841
RsaI GTAC 4 cut(s) 291, 1217, 1244, 1478
RsaNI GTAC 4 cut(s) 290, 1216, 1243, 1477
RseI CAYNNNNRTG 5 cut(s) 138, 144, 368, 1401, 2022
SalI GTCGAC 2 cut(s) 1688, 1765
SaqAI TTAA 8 cut(s) 582, 822, 941, 999, 1431, 1524, 1667, 2044
SatI GCNGC 8 cut(s) 39, 42, 432, 473, 551, 1636, 1915, 2014
Sau3AI GATC 9 cut(s) 108, 591, 612, 747, 784, 888, 1086, 1461, 1552
Sau96I GGNCC 4 cut(s) 1223, 1644, 1834, 2027
SchI GAGTC 3 cut(s) 124, 531, 1685
ScrFI CCNGG 4 cut(s) 342, 669, 1221, 1932
SduI GDGCHC 2 cut(s) 302, 1970
SfaNI GCATC 5 cut(s) 315, 425, 630, 692, 1245
SfcI CTRYAG 1 cut(s) 1227
SinI GGWCC 4 cut(s) 1223, 1644, 1834, 2027
SmiMI CAYNNNNRTG 5 cut(s) 138, 144, 368, 1401, 2022
SmlI CTYRAG 2 cut(s) 194, 536
SmoI CTYRAG 2 cut(s) 194, 536
SpeI ACTAGT 1 cut(s) 761
SphI GCATGC 1 cut(s) 203
SseBI AGGCCT 2 cut(s) 9, 1358
SsiI CCGC 3 cut(s) 39, 1494, 1636
SspI AATATT 1 cut(s) 945
SspMI CTAG 7 cut(s) 5, 11, 428, 762, 788, 1049, 1631
StuI AGGCCT 2 cut(s) 9, 1358
StyD4I CCNGG 4 cut(s) 340, 667, 1219, 1930
StyI CCWWGG 2 cut(s) 30, 153
TaaI ACNGT 9 cut(s) 370, 727, 997, 1200, 1210, 1392, 1622, 1771, 1982
TaiI ACGT 1 cut(s) 1509
TaqII GACCGA 1 cut(s) 1684
TatI WGTACW 2 cut(s) 1242, 1476
TauI GCSGC 2 cut(s) 41, 1638
TfiI GAWTC 6 cut(s) 164, 312, 835, 1075, 1513, 1955
Tru1I TTAA 8 cut(s) 582, 822, 941, 999, 1431, 1524, 1667, 2044
Tru9I TTAA 8 cut(s) 582, 822, 941, 999, 1431, 1524, 1667, 2044
TscAI CASTG 6 cut(s) 375, 426, 730, 1705, 1755, 2026
TseFI GTSAC 3 cut(s) 344, 1392, 1777
TseI GCWGC 6 cut(s) 41, 431, 472, 550, 1914, 2013
Tsp45I GTSAC 3 cut(s) 344, 1392, 1777
TspDTI ATGAA 6 cut(s) 163, 234, 390, 1027, 1307, 1976
TspGWI ACGGA 5 cut(s) 560, 1073, 1414, 1449, 1956
TspRI CASTG 6 cut(s) 375, 426, 730, 1705, 1755, 2026
Tth111I GACNNNGTC 2 cut(s) 1694, 1838
VneI GTGCAC 1 cut(s) 298
VpaK11BI GGWCC 4 cut(s) 1223, 1644, 1834, 2027
VspI ATTAAT 1 cut(s) 941
XagI CCTNNNNNAGG 1 cut(s) 1230
XapI RAATTY 4 cut(s) 455, 662, 1437, 1519
XbaI TCTAGA 1 cut(s) 1048
XceI RCATGY 2 cut(s) 203, 248
XmiI GTMKAC 2 cut(s) 1689, 1766
XmnI GAANNNNTTC 2 cut(s) 693, 899
XspI CTAG 7 cut(s) 5, 11, 428, 762, 788, 1049, 1631
Zsp2I ATGCAT 1 cut(s) 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.