Sieve element occlusion
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 742 |
| Acc36I | ACCTGC | 1 cut(s) | 1081 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 977 |
| AccII | CGCG | 1 cut(s) | 994 |
| AciI | CCGC | 3 cut(s) | 315, 873, 1379 |
| AclWI | GGATC | 1 cut(s) | 149 |
| AcoI | YGGCCR | 1 cut(s) | 495 |
| AcsI | RAATTY | 1 cut(s) | 1021 |
| AcuI | CTGAAG | 2 cut(s) | 1020, 1133 |
| AcyI | GRCGYC | 4 cut(s) | 1061, 1455, 1488, 1521 |
| AfaI | GTAC | 3 cut(s) | 433, 477, 1304 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 215, 634, 977 |
| AflIII | ACRYGT | 1 cut(s) | 478 |
| AgsI | TTSAA | 6 cut(s) | 332, 425, 912, 1157, 1291, 1312 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 781 |
| AhlI | ACTAGT | 1 cut(s) | 254 |
| AjnI | CCWGG | 3 cut(s) | 403, 492, 1330 |
| Alw21I | GWGCWC | 2 cut(s) | 624, 753 |
| Alw26I | GTCTC | 3 cut(s) | 165, 634, 654 |
| AlwI | GGATC | 1 cut(s) | 149 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 357 |
| Ama87I | CYCGRG | 1 cut(s) | 944 |
| AoxI | GGCC | 3 cut(s) | 217, 489, 495 |
| ApeKI | GCWGC | 2 cut(s) | 312, 381 |
| ApoI | RAATTY | 1 cut(s) | 1021 |
| AspLEI | GCGC | 2 cut(s) | 743, 994 |
| AspS9I | GGNCC | 2 cut(s) | 217, 490 |
| AsuHPI | GGTGA | 8 cut(s) | 119, 125, 131, 137, 419, 665, 1399, 1420 |
| AvaI | CYCGRG | 1 cut(s) | 944 |
| BanII | GRGCYC | 1 cut(s) | 753 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1346, 1378 |
| Bbv12I | GWGCWC | 2 cut(s) | 624, 753 |
| BbvI | GCAGC | 2 cut(s) | 324, 368 |
| BccI | CCATC | 3 cut(s) | 82, 1070, 1219 |
| BceAI | ACGGC | 2 cut(s) | 482, 1282 |
| BciT130I | CCWGG | 3 cut(s) | 405, 494, 1332 |
| BciVI | GTATCC | 1 cut(s) | 688 |
| BclI | TGATCA | 1 cut(s) | 100 |
| BcoDI | GTCTC | 3 cut(s) | 165, 634, 654 |
| BcuI | ACTAGT | 1 cut(s) | 254 |
| BfaI | CTAG | 1 cut(s) | 255 |
| BfuAI | ACCTGC | 1 cut(s) | 1081 |
| BfuI | GTATCC | 1 cut(s) | 688 |
| BisI | GCNGC | 3 cut(s) | 313, 382, 873 |
| BlsI | GCNGC | 3 cut(s) | 314, 383, 874 |
| Bme1390I | CCNGG | 3 cut(s) | 405, 494, 1332 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 781 |
| BmeT110I | CYCGRG | 1 cut(s) | 944 |
| BmgT120I | GGNCC | 2 cut(s) | 217, 490 |
| BmiI | GGNNCC | 2 cut(s) | 1233, 1253 |
| BmrFI | CCNGG | 3 cut(s) | 405, 494, 1332 |
| BmsI | GCATC | 3 cut(s) | 559, 1284, 1344 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1064 |
| BpmI | CTGGAG | 1 cut(s) | 334 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1035 |
| BpuEI | CTTGAG | 3 cut(s) | 650, 857, 1295 |
| BsaAI | YACGTR | 1 cut(s) | 431 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1210 |
| BsaHI | GRCGYC | 4 cut(s) | 1061, 1455, 1488, 1521 |
| BsaI | GGTCTC | 1 cut(s) | 165 |
| BsaJI | CCNNGG | 4 cut(s) | 33, 492, 945, 1298 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 1392, 1422 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 215, 634, 977 |
| Bse1I | ACTGG | 4 cut(s) | 719, 765, 1184, 1395 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1210 |
| BseBI | CCWGG | 3 cut(s) | 405, 494, 1332 |
| BseDI | CCNNGG | 4 cut(s) | 33, 492, 945, 1298 |
| BseGI | GGATG | 3 cut(s) | 624, 822, 1030 |
| BseJI | GATNNNNATC | 1 cut(s) | 1210 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 215, 634, 977 |
| BseMII | CTCAG | 1 cut(s) | 738 |
| BseNI | ACTGG | 4 cut(s) | 719, 765, 1184, 1395 |
| BseRI | GAGGAG | 1 cut(s) | 50 |
| BseXI | GCAGC | 2 cut(s) | 324, 368 |
| BsgI | GTGCAG | 1 cut(s) | 343 |
| Bsh1236I | CGCG | 1 cut(s) | 994 |
| BshFI | GGCC | 3 cut(s) | 219, 491, 497 |
| BsiHKAI | GWGCWC | 2 cut(s) | 624, 753 |
| BsiHKCI | CYCGRG | 1 cut(s) | 944 |
| BsiWI | CGTACG | 1 cut(s) | 431 |
| BslFI | GGGAC | 3 cut(s) | 855, 963, 1072 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 215, 634, 977 |
| BsmAI | GTCTC | 3 cut(s) | 165, 634, 654 |
| BsmFI | GGGAC | 3 cut(s) | 855, 963, 1072 |
| BsnI | GGCC | 3 cut(s) | 219, 491, 497 |
| Bso31I | GGTCTC | 1 cut(s) | 165 |
| BsoBI | CYCGRG | 1 cut(s) | 944 |
| Bsp1286I | GDGCHC | 2 cut(s) | 624, 753 |
| Bsp143I | GATC | 5 cut(s) | 100, 141, 285, 655, 790 |
| BspACI | CCGC | 3 cut(s) | 315, 873, 1379 |
| BspANI | GGCC | 3 cut(s) | 219, 491, 497 |
| BspCNI | CTCAG | 1 cut(s) | 739 |
| BspFNI | CGCG | 1 cut(s) | 994 |
| BspHI | TCATGA | 2 cut(s) | 27, 174 |
| BspLI | GGNNCC | 2 cut(s) | 1233, 1253 |
| BspMI | ACCTGC | 1 cut(s) | 1081 |
| BspPI | GGATC | 1 cut(s) | 149 |
| BspTNI | GGTCTC | 1 cut(s) | 165 |
| BsrI | ACTGG | 4 cut(s) | 719, 765, 1184, 1395 |
| BssECI | CCNNGG | 4 cut(s) | 33, 492, 945, 1298 |
| BssMI | GATC | 5 cut(s) | 100, 141, 285, 655, 790 |
| BssNI | GRCGYC | 4 cut(s) | 1061, 1455, 1488, 1521 |
| Bst2UI | CCWGG | 3 cut(s) | 405, 494, 1332 |
| Bst4CI | ACNGT | 2 cut(s) | 203, 665 |
| Bst6I | CTCTTC | 1 cut(s) | 762 |
| BstACI | GRCGYC | 4 cut(s) | 1061, 1455, 1488, 1521 |
| BstBAI | YACGTR | 1 cut(s) | 431 |
| BstC8I | GCNNGC | 5 cut(s) | 226, 346, 739, 996, 1377 |
| BstDEI | CTNAG | 2 cut(s) | 747, 1035 |
| BstDSI | CCRYGG | 1 cut(s) | 1298 |
| BstF5I | GGATG | 3 cut(s) | 624, 822, 1030 |
| BstFNI | CGCG | 1 cut(s) | 994 |
| BstHHI | GCGC | 2 cut(s) | 743, 994 |
| BstKTI | GATC | 5 cut(s) | 103, 144, 288, 658, 793 |
| BstMAI | GTCTC | 3 cut(s) | 165, 634, 654 |
| BstMBI | GATC | 5 cut(s) | 100, 141, 285, 655, 790 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 225, 503, 899 |
| BstNI | CCWGG | 3 cut(s) | 405, 494, 1332 |
| BstNSI | RCATGY | 1 cut(s) | 482 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1064 |
| BstSCI | CCNGG | 3 cut(s) | 403, 492, 1330 |
| BstUI | CGCG | 1 cut(s) | 994 |
| BstV1I | GCAGC | 2 cut(s) | 324, 368 |
| BsuI | GTATCC | 1 cut(s) | 688 |
| BsuRI | GGCC | 3 cut(s) | 219, 491, 497 |
| BtgI | CCRYGG | 1 cut(s) | 1298 |
| BtgZI | GCGATG | 1 cut(s) | 537 |
| BtsCI | GGATG | 3 cut(s) | 624, 822, 1030 |
| BtsI | GCAGTG | 1 cut(s) | 1253 |
| BtsIMutI | CAGTG | 4 cut(s) | 670, 772, 1253, 1402 |
| BveI | ACCTGC | 1 cut(s) | 1081 |
| Cac8I | GCNNGC | 5 cut(s) | 226, 346, 739, 996, 1377 |
| CaiI | CAGNNNCTG | 1 cut(s) | 357 |
| CciI | TCATGA | 2 cut(s) | 27, 174 |
| CfoI | GCGC | 2 cut(s) | 743, 994 |
| Cfr13I | GGNCC | 2 cut(s) | 217, 490 |
| CseI | GACGC | 4 cut(s) | 1069, 1463, 1496, 1529 |
| Csp6I | GTAC | 3 cut(s) | 432, 476, 1303 |
| CviAII | CATG | 9 cut(s) | 28, 82, 175, 221, 239, 468, 479, 1345, 1445 |
| CviQI | GTAC | 3 cut(s) | 432, 476, 1303 |
| DdeI | CTNAG | 2 cut(s) | 747, 1035 |
| DpnI | GATC | 5 cut(s) | 102, 143, 287, 657, 792 |
| DpnII | GATC | 5 cut(s) | 100, 141, 285, 655, 790 |
| DraI | TTTAAA | 1 cut(s) | 1537 |
| DriI | GACNNNNNGTC | 1 cut(s) | 781 |
| EaeI | YGGCCR | 1 cut(s) | 495 |
| Eam1104I | CTCTTC | 1 cut(s) | 762 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 781 |
| EarI | CTCTTC | 1 cut(s) | 762 |
| Ecl136II | GAGCTC | 1 cut(s) | 751 |
| Eco24I | GRGCYC | 1 cut(s) | 753 |
| Eco31I | GGTCTC | 1 cut(s) | 165 |
| Eco53kI | GAGCTC | 1 cut(s) | 751 |
| Eco57I | CTGAAG | 2 cut(s) | 1020, 1133 |
| Eco88I | CYCGRG | 1 cut(s) | 944 |
| EcoICRI | GAGCTC | 1 cut(s) | 751 |
| EcoRII | CCWGG | 3 cut(s) | 403, 492, 1330 |
| EcoT22I | ATGCAT | 1 cut(s) | 421 |
| EcoT38I | GRGCYC | 1 cut(s) | 753 |
| FaeI | CATG | 9 cut(s) | 31, 85, 178, 224, 242, 471, 482, 1348, 1448 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 429, 461 |
| FaqI | GGGAC | 3 cut(s) | 855, 963, 1072 |
| FatI | CATG | 9 cut(s) | 27, 81, 174, 220, 238, 467, 478, 1344, 1444 |
| FauNDI | CATATG | 1 cut(s) | 787 |
| FbaI | TGATCA | 1 cut(s) | 100 |
| Fnu4HI | GCNGC | 3 cut(s) | 313, 382, 873 |
| FokI | GGATG | 3 cut(s) | 611, 809, 1017 |
| FriOI | GRGCYC | 1 cut(s) | 753 |
| Fsp4HI | GCNGC | 3 cut(s) | 313, 382, 873 |
| FspBI | CTAG | 1 cut(s) | 255 |
| FspI | TGCGCA | 1 cut(s) | 742 |
| GlaI | GCGC | 2 cut(s) | 742, 993 |
| GluI | GCNGC | 3 cut(s) | 313, 382, 873 |
| GsuI | CTGGAG | 1 cut(s) | 334 |
| HaeIII | GGCC | 3 cut(s) | 219, 491, 497 |
| HgaI | GACGC | 4 cut(s) | 1069, 1463, 1496, 1529 |
| HhaI | GCGC | 2 cut(s) | 743, 994 |
| Hin1I | GRCGYC | 4 cut(s) | 1061, 1455, 1488, 1521 |
| Hin1II | CATG | 9 cut(s) | 31, 85, 178, 224, 242, 471, 482, 1348, 1448 |
| Hin6I | GCGC | 2 cut(s) | 741, 992 |
| HinP1I | GCGC | 2 cut(s) | 741, 992 |
| HincII | GTYRAC | 1 cut(s) | 598 |
| HindII | GTYRAC | 1 cut(s) | 598 |
| HindIII | AAGCTT | 1 cut(s) | 833 |
| HinfI | GANTC | 5 cut(s) | 275, 400, 425, 896, 940 |
| HphI | GGTGA | 8 cut(s) | 119, 125, 131, 137, 419, 665, 1399, 1420 |
| Hpy166II | GTNNAC | 4 cut(s) | 137, 598, 668, 1305 |
| Hpy188I | TCNGA | 4 cut(s) | 261, 1144, 1179, 1221 |
| Hpy188III | TCNNGA | 7 cut(s) | 28, 175, 209, 250, 659, 944, 1073 |
| Hpy8I | GTNNAC | 4 cut(s) | 137, 598, 668, 1305 |
| HpyAV | CCTTC | 4 cut(s) | 116, 122, 1079, 1114 |
| HpyCH4III | ACNGT | 2 cut(s) | 203, 665 |
| HpyCH4IV | ACGT | 3 cut(s) | 430, 976, 1510 |
| HpyCH4V | TGCA | 5 cut(s) | 191, 360, 384, 419, 572 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 225, 503, 899 |
| HpyF3I | CTNAG | 2 cut(s) | 747, 1035 |
| HpySE526I | ACGT | 3 cut(s) | 430, 976, 1510 |
| Hsp92I | GRCGYC | 4 cut(s) | 1061, 1455, 1488, 1521 |
| Hsp92II | CATG | 9 cut(s) | 31, 85, 178, 224, 242, 471, 482, 1348, 1448 |
| HspAI | GCGC | 2 cut(s) | 741, 992 |
| Ksp22I | TGATCA | 1 cut(s) | 100 |
| Kzo9I | GATC | 5 cut(s) | 100, 141, 285, 655, 790 |
| LmnI | GCTCC | 2 cut(s) | 353, 1243 |
| Lsp1109I | GCAGC | 2 cut(s) | 324, 368 |
| LweI | GCATC | 3 cut(s) | 559, 1284, 1344 |
| MaeI | CTAG | 1 cut(s) | 255 |
| MaeII | ACGT | 3 cut(s) | 430, 976, 1510 |
| MaeIII | GTNAC | 3 cut(s) | 407, 1134, 1163 |
| MalI | GATC | 5 cut(s) | 102, 143, 287, 657, 792 |
| MboI | GATC | 5 cut(s) | 100, 141, 285, 655, 790 |
| MboII | GAAGA | 6 cut(s) | 449, 530, 749, 1169, 1215, 1303 |
| MhlI | GDGCHC | 2 cut(s) | 624, 753 |
| MluCI | AATT | 8 cut(s) | 196, 645, 674, 687, 984, 1021, 1040, 1360 |
| MlyI | GAGTC | 1 cut(s) | 409 |
| MmeI | TCCRAC | 3 cut(s) | 73, 615, 1346 |
| Mph1103I | ATGCAT | 1 cut(s) | 421 |
| MslI | CAYNNNNRTG | 2 cut(s) | 86, 1312 |
| MspA1I | CMGCKG | 1 cut(s) | 315 |
| MspR9I | CCNGG | 3 cut(s) | 405, 494, 1332 |
| MvaI | CCWGG | 3 cut(s) | 405, 494, 1332 |
| MvnI | CGCG | 1 cut(s) | 994 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 225, 503, 899 |
| NdeI | CATATG | 1 cut(s) | 787 |
| NdeII | GATC | 5 cut(s) | 100, 141, 285, 655, 790 |
| NlaIII | CATG | 9 cut(s) | 31, 85, 178, 224, 242, 471, 482, 1348, 1448 |
| NlaIV | GGNNCC | 2 cut(s) | 1233, 1253 |
| NmuCI | GTSAC | 1 cut(s) | 407 |
| NsbI | TGCGCA | 1 cut(s) | 742 |
| NsiI | ATGCAT | 1 cut(s) | 421 |
| NspI | RCATGY | 1 cut(s) | 482 |
| PagI | TCATGA | 2 cut(s) | 27, 174 |
| PciI | ACATGT | 1 cut(s) | 478 |
| PfeI | GAWTC | 4 cut(s) | 275, 425, 896, 940 |
| Pfl23II | CGTACG | 1 cut(s) | 431 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 977 |
| PkrI | GCNGC | 3 cut(s) | 314, 383, 874 |
| PleI | GAGTC | 1 cut(s) | 408 |
| PpsI | GAGTC | 1 cut(s) | 408 |
| Ppu21I | YACGTR | 1 cut(s) | 431 |
| PscI | ACATGT | 1 cut(s) | 478 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1064 |
| Psp124BI | GAGCTC | 1 cut(s) | 753 |
| Psp6I | CCWGG | 3 cut(s) | 403, 492, 1330 |
| PspGI | CCWGG | 3 cut(s) | 403, 492, 1330 |
| PspLI | CGTACG | 1 cut(s) | 431 |
| PspN4I | GGNNCC | 2 cut(s) | 1233, 1253 |
| PspPI | GGNCC | 2 cut(s) | 217, 490 |
| PstNI | CAGNNNCTG | 1 cut(s) | 357 |
| RsaI | GTAC | 3 cut(s) | 433, 477, 1304 |
| RsaNI | GTAC | 3 cut(s) | 432, 476, 1303 |
| RseI | CAYNNNNRTG | 2 cut(s) | 86, 1312 |
| SacI | GAGCTC | 1 cut(s) | 753 |
| SatI | GCNGC | 3 cut(s) | 313, 382, 873 |
| Sau3AI | GATC | 5 cut(s) | 100, 141, 285, 655, 790 |
| Sau96I | GGNCC | 2 cut(s) | 217, 490 |
| SchI | GAGTC | 1 cut(s) | 409 |
| ScrFI | CCNGG | 3 cut(s) | 405, 494, 1332 |
| SduI | GDGCHC | 2 cut(s) | 624, 753 |
| SfaNI | GCATC | 3 cut(s) | 559, 1284, 1344 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 86, 1312 |
| SmlI | CTYRAG | 3 cut(s) | 629, 836, 1274 |
| SmoI | CTYRAG | 3 cut(s) | 629, 836, 1274 |
| SpeI | ACTAGT | 1 cut(s) | 254 |
| Sse9I | AATT | 8 cut(s) | 196, 645, 674, 687, 984, 1021, 1040, 1360 |
| SsiI | CCGC | 3 cut(s) | 315, 873, 1379 |
| SspI | AATATT | 1 cut(s) | 298 |
| SspMI | CTAG | 1 cut(s) | 255 |
| SstI | GAGCTC | 1 cut(s) | 753 |
| StyD4I | CCNGG | 3 cut(s) | 403, 492, 1330 |
| TaaI | ACNGT | 2 cut(s) | 203, 665 |
| TaiI | ACGT | 3 cut(s) | 433, 979, 1513 |
| TaqI | TCGA | 7 cut(s) | 153, 251, 548, 658, 988, 1101, 1283 |
| TaqII | GACCGA | 1 cut(s) | 47 |
| TasI | AATT | 8 cut(s) | 196, 645, 674, 687, 984, 1021, 1040, 1360 |
| TatI | WGTACW | 1 cut(s) | 475 |
| TauI | GCSGC | 1 cut(s) | 875 |
| TfiI | GAWTC | 4 cut(s) | 275, 425, 896, 940 |
| TscAI | CASTG | 4 cut(s) | 670, 772, 1253, 1402 |
| TseFI | GTSAC | 1 cut(s) | 407 |
| TseI | GCWGC | 2 cut(s) | 312, 381 |
| Tsp45I | GTSAC | 1 cut(s) | 407 |
| TspDTI | ATGAA | 9 cut(s) | 191, 227, 531, 811, 1034, 1161, 1278, 1361, 1431 |
| TspGWI | ACGGA | 2 cut(s) | 154, 449 |
| TspRI | CASTG | 4 cut(s) | 670, 772, 1253, 1402 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 977 |
| XapI | RAATTY | 1 cut(s) | 1021 |
| XceI | RCATGY | 1 cut(s) | 482 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1402 |
| XspI | CTAG | 1 cut(s) | 255 |
| Zsp2I | ATGCAT | 1 cut(s) | 421 |