Rw1G002330

Sieve element occlusion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0000511
Physical Location & Seq
Reverse (-)
4131761 .. 4133557
1797 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G002330.1

Sequence Viewer

Length: 864 bp
ATGGACAATATTAACGACACAGACATTTCAAGACTCAAAGAATTCGATGAGCGAGTTGGACAGACTTTTCGAAATTGTAAAATTGTGTGGATCCCCATTGTGGAGGATTGGACTAAAGAGGGAATAGAAGAAGCGTTCAAGGAATGGAGATCTAAGATGCCATGGTATGCAGTCCAGTACTTTTCACCTGCAGCCATCGAGTTCCTCAAGGAATGGGGCTTCGAAGGTAATCCTATTGGCGTGCTGATGAATGAAAAGGGAGAAATTGAAGACAAGAAAGCACTCGACGTGATTCGTTCACTTGGAGGATCTGCAATTTATACCTTTTTTAAGCCGTTCAATAAAGATTTTACTGAAGTCTATCATGCATCTGCGAAAGTTGTAGAGACTCTATTTTATCCCAATGATAAGTCACCCCGCAAGCTCTTTAAAGTTACTACTAATGGGAATGTGGCACTCGCTACGACAATGGTTAGCCTCAAACAGCTGGAGCGGAAGAATCTGTTTTTGCTGATTTCAAGAGTCAATATTTCTCCAGATGTCATAACATATCTTAAGCCACTTCATCATAAATTGGAGACAACTGCAAGTGATTATAAGATCGTGTGGATCCCTATCGCAGAGAAGGTTGACGATGCAAAACAACAACTATTTTCCAAAAAGAGGTTGACGATGCCATGGTACGCGATGTATACTTTAGCACCCATACCAAACATCCGGTACATCAAAACAGAGTGGGCGTGGAGAAATAAGCCTATGGTAGTCTTGTTGACCTGTCGAGGCAACGCCAAATACAGAGTTGAAATTGAGTCGAATAAGCGATCTGCAGTCGACACATTCCGTTTACTAAATGAGCTTCCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SEO_C SEO_N

Protein Analysis

287

Amino Acids

33.5

Weight (kDa)

9.15

Isoelectric Point (pI)

39.44

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000511)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g02370 FvH4_4g02370 FvH4_4g02370 FvH4_4g02430 FvH4_4g02430 FvH4_4g02460 FvH4_4g02460 FvH4_4g02460 FvH4_4g02480 FvH4_4g02480 FvH4_4g02480 FvH4_4g02480 FvH4_6g05920
malus_domestica MD00G1111000.v1.1 MD08G1225800.v1.1 MD13G1205700.v1.1 MD13G1205800.v1.1 MD13G1205900.v1.1 MD13G1206000.v1.1 MD16G1207500.v1.1 MD16G1207600.v1.1
prunus_persica Prupe.1G028400_v2.0.a1 Prupe.1G028500_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028900_v2.0.a1 Prupe.1G029000_v2.0.a1 Prupe.1G029100_v2.0.a1
pyrus_communis pycom08g19680 pycom08g19730 pycom16g17460 pycom16g17500
rosa_chinensis RchiOBHm_Chr4g0389751 RchiOBHm_Chr4g0389771 RchiOBHm_Chr4g0389791 RchiOBHm_Chr4g0389861 RchiOBHm_Chr4g0389881 RchiOBHm_Chr4g0389901 RchiOBHm_Chr4g0412411
rosa_laevigata RLG00000008288 RLG00000009901 RLG00000009945 RLG00000009954 RLG00000009956 RLG00000009957 RLG00000009958 RLG00000019246
rosa_multiflora Rmu_co8275125.1_g000001 Rmu_sc0002083.1_g000003 Rmu_sc0002083.1_g000025 Rmu_sc0002604.1_g000016 Rmu_sc0004336.1_g000031 Rmu_sc0010423.1_g000003
rosa_roxburghii Rroxscaffold_5G00335790 Rroxscaffold_5G00335850 Rroxscaffold_5G00335970 Rroxscaffold_5G00335990
rosa_rugosa Rorug03G0325100 Rorug03G0325300 Rorug03G0325500.1 Rorug03G0326800 Rorug03G0327000 Rorug04G0104400
rosa_samantha Rh4AG029900 Rh4AG030000 Rh4AG030100 Rh4AG030200 Rh4AG030600 Rh4AG172900 Rh4BG023200 Rh4BG023900 Rh4BG024000 Rh4BG024100 Rh4BG172200 Rh4DG023300 Rh4DG023800 Rh4DG023900 Rh4DG024000 Rh4DG151600
rosa_wichuraiana Rw1G002330 Rw1G002340 Rw4G002260 Rw4G002270 Rw4G002280 Rw4G002300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 597
AarI CACCTGC 1 cut(s) 196
Acc36I ACCTGC 1 cut(s) 196
AccBSI CCGCTC 1 cut(s) 493
AccI GTMKAC 2 cut(s) 692, 831
AccII CGCG 1 cut(s) 686
AciI CCGC 2 cut(s) 418, 493
AclWI GGATC 5 cut(s) 85, 98, 316, 604, 617
AcsI RAATTY 1 cut(s) 41
AcuI CTGAAG 1 cut(s) 375
AfaI GTAC 3 cut(s) 179, 683, 722
AfiI CCNNNNNNNGG 2 cut(s) 100, 663
AflII CTTAAG 1 cut(s) 554
AgsI TTSAA 6 cut(s) 30, 139, 269, 340, 519, 803
AjiI CACGTC 1 cut(s) 289
AluBI AGCT 3 cut(s) 424, 487, 856
AluI AGCT 3 cut(s) 424, 487, 856
Alw26I GTCTC 2 cut(s) 380, 572
AlwI GGATC 5 cut(s) 85, 98, 316, 604, 617
ApeKI GCWGC 1 cut(s) 191
ApoI RAATTY 1 cut(s) 41
ArsI GACNNNNNNTTYG 2 cut(s) 51, 83
AsuHPI GGTGA 2 cut(s) 177, 405
AsuII TTCGAA 2 cut(s) 70, 222
BamHI GGATCC 2 cut(s) 90, 609
BbsI GAAGAC 1 cut(s) 276
BbvI GCAGC 1 cut(s) 203
BccI CCATC 1 cut(s) 203
BceAI ACGGC 1 cut(s) 319
BcoDI GTCTC 2 cut(s) 380, 572
BfmI CTRYAG 2 cut(s) 189, 825
BfrI CTTAAG 1 cut(s) 554
BfuAI ACCTGC 1 cut(s) 196
BglII AGATCT 1 cut(s) 149
BisI GCNGC 1 cut(s) 192
BlsI GCNGC 1 cut(s) 193
BmcAI AGTACT 1 cut(s) 179
BmgBI CACGTC 1 cut(s) 289
BmiI GGNNCC 2 cut(s) 92, 611
BmsI GCATC 4 cut(s) 147, 377, 625, 663
BpiI GAAGAC 1 cut(s) 276
BpmI CTGGAG 2 cut(s) 509, 519
Bpu14I TTCGAA 2 cut(s) 70, 222
BpuEI CTTGAG 1 cut(s) 191
BsaBI GATNNNNATC 1 cut(s) 614
BsaJI CCNNGG 2 cut(s) 161, 677
BsaWI WCCGGW 1 cut(s) 717
Bsc4I CCNNNNNNNGG 2 cut(s) 100, 663
Bse1I ACTGG 1 cut(s) 175
Bse8I GATNNNNATC 1 cut(s) 614
BseDI CCNNGG 2 cut(s) 161, 677
BseGI GGATG 1 cut(s) 714
BseJI GATNNNNATC 1 cut(s) 614
BseLI CCNNNNNNNGG 2 cut(s) 100, 663
BseNI ACTGG 1 cut(s) 175
BseXI GCAGC 1 cut(s) 203
Bsh1236I CGCG 1 cut(s) 686
BsiSI CCGG 1 cut(s) 718
BslI CCNNNNNNNGG 2 cut(s) 100, 663
BsmAI GTCTC 2 cut(s) 380, 572
Bsp119I TTCGAA 2 cut(s) 70, 222
Bsp143I GATC 6 cut(s) 90, 149, 308, 600, 609, 821
Bsp19I CCATGG 2 cut(s) 161, 677
BspACI CCGC 2 cut(s) 418, 493
BspFNI CGCG 1 cut(s) 686
BspLI GGNNCC 2 cut(s) 92, 611
BspMAI CTGCAG 2 cut(s) 193, 829
BspMI ACCTGC 1 cut(s) 196
BspPI GGATC 5 cut(s) 85, 98, 316, 604, 617
BspT104I TTCGAA 2 cut(s) 70, 222
BspTI CTTAAG 1 cut(s) 554
BsrBI CCGCTC 1 cut(s) 493
BsrI ACTGG 1 cut(s) 175
BssECI CCNNGG 2 cut(s) 161, 677
BssMI GATC 6 cut(s) 90, 149, 308, 600, 609, 821
BssNAI GTATAC 1 cut(s) 693
BssT1I CCWWGG 2 cut(s) 161, 677
Bst1107I GTATAC 1 cut(s) 693
BstAFI CTTAAG 1 cut(s) 554
BstBI TTCGAA 2 cut(s) 70, 222
BstC8I GCNNGC 2 cut(s) 242, 422
BstDEI CTNAG 2 cut(s) 153, 861
BstDSI CCRYGG 2 cut(s) 161, 677
BstF5I GGATG 1 cut(s) 714
BstFNI CGCG 1 cut(s) 686
BstKTI GATC 6 cut(s) 93, 152, 311, 603, 612, 824
BstMAI GTCTC 2 cut(s) 380, 572
BstMBI GATC 6 cut(s) 90, 149, 308, 600, 609, 821
BstSFI CTRYAG 2 cut(s) 189, 825
BstUI CGCG 1 cut(s) 686
BstV1I GCAGC 1 cut(s) 203
BstV2I GAAGAC 1 cut(s) 276
BstX2I RGATCY 4 cut(s) 90, 149, 308, 609
BstYI RGATCY 4 cut(s) 90, 149, 308, 609
BstZ17I GTATAC 1 cut(s) 693
BtgI CCRYGG 2 cut(s) 161, 677
BtgZI GCGATG 1 cut(s) 701
BtrI CACGTC 1 cut(s) 289
BtsCI GGATG 1 cut(s) 714
BveI ACCTGC 1 cut(s) 196
Cac8I GCNNGC 2 cut(s) 242, 422
Csp6I GTAC 3 cut(s) 178, 682, 721
CviAII CATG 3 cut(s) 162, 365, 678
CviJI RGCY 9 cut(s) 194, 219, 334, 424, 477, 487, 559, 754, 856
CviKI_1 RGCY 9 cut(s) 194, 219, 334, 424, 477, 487, 559, 754, 856
CviQI GTAC 3 cut(s) 178, 682, 721
DdeI CTNAG 2 cut(s) 153, 861
DpnI GATC 6 cut(s) 92, 151, 310, 602, 611, 823
DpnII GATC 6 cut(s) 90, 149, 308, 600, 609, 821
DraI TTTAAA 1 cut(s) 430
Eco130I CCWWGG 2 cut(s) 161, 677
Eco57I CTGAAG 1 cut(s) 375
EcoRI GAATTC 1 cut(s) 41
EcoT14I CCWWGG 2 cut(s) 161, 677
EcoT22I ATGCAT 1 cut(s) 370
ErhI CCWWGG 2 cut(s) 161, 677
FaeI CATG 3 cut(s) 165, 368, 681
FatI CATG 3 cut(s) 161, 364, 677
FauI CCCGC 1 cut(s) 425
FblI GTMKAC 2 cut(s) 692, 831
Fnu4HI GCNGC 1 cut(s) 192
FokI GGATG 1 cut(s) 701
Fsp4HI GCNGC 1 cut(s) 192
GluI GCNGC 1 cut(s) 192
GsuI CTGGAG 2 cut(s) 509, 519
HapII CCGG 1 cut(s) 718
Hin1II CATG 3 cut(s) 165, 368, 681
HincII GTYRAC 4 cut(s) 631, 669, 771, 832
HindII GTYRAC 4 cut(s) 631, 669, 771, 832
HinfI GANTC 6 cut(s) 33, 292, 388, 499, 522, 809
HpaII CCGG 1 cut(s) 718
HphI GGTGA 2 cut(s) 177, 405
Hpy166II GTNNAC 7 cut(s) 299, 631, 669, 693, 771, 832, 845
Hpy188III TCNNGA 3 cut(s) 30, 519, 536
Hpy8I GTNNAC 7 cut(s) 299, 631, 669, 693, 771, 832, 845
Hpy99I CGWCG 1 cut(s) 290
HpyAV CCTTC 2 cut(s) 218, 619
HpyCH4IV ACGT 1 cut(s) 288
HpyCH4V TGCA 7 cut(s) 170, 191, 314, 368, 587, 638, 827
HpyF3I CTNAG 2 cut(s) 153, 861
HpySE526I ACGT 1 cut(s) 288
Hsp92II CATG 3 cut(s) 165, 368, 681
Kzo9I GATC 6 cut(s) 90, 149, 308, 600, 609, 821
LmnI GCTCC 1 cut(s) 490
LpnPI CCDG 6 cut(s) 188, 201, 473, 549, 731, 787
Lsp1109I GCAGC 1 cut(s) 203
LweI GCATC 4 cut(s) 147, 377, 625, 663
MaeII ACGT 1 cut(s) 288
MaeIII GTNAC 2 cut(s) 411, 433
MalI GATC 6 cut(s) 92, 151, 310, 602, 611, 823
MbiI CCGCTC 1 cut(s) 493
MboI GATC 6 cut(s) 90, 149, 308, 600, 609, 821
MboII GAAGA 3 cut(s) 140, 281, 508
MflI RGATCY 4 cut(s) 90, 149, 308, 609
MluCI AATT 7 cut(s) 41, 73, 81, 264, 315, 572, 804
MlyI GAGTC 4 cut(s) 27, 382, 531, 818
MmeI TCCRAC 1 cut(s) 37
MnlI CCTC 7 cut(s) 97, 112, 215, 299, 488, 657, 773
Mph1103I ATGCAT 1 cut(s) 370
MseI TTAA 4 cut(s) 12, 330, 429, 555
MspA1I CMGCKG 1 cut(s) 487
MspCI CTTAAG 1 cut(s) 554
MspI CCGG 1 cut(s) 718
MvnI CGCG 1 cut(s) 686
NcoI CCATGG 2 cut(s) 161, 677
NdeII GATC 6 cut(s) 90, 149, 308, 600, 609, 821
NlaIII CATG 3 cut(s) 165, 368, 681
NlaIV GGNNCC 2 cut(s) 92, 611
NmuCI GTSAC 1 cut(s) 411
NsiI ATGCAT 1 cut(s) 370
NspV TTCGAA 2 cut(s) 70, 222
PaqCI CACCTGC 1 cut(s) 196
PfeI GAWTC 2 cut(s) 292, 499
PkrI GCNGC 1 cut(s) 193
PleI GAGTC 4 cut(s) 27, 382, 530, 817
PpsI GAGTC 4 cut(s) 27, 382, 530, 817
PsiI TTATAA 1 cut(s) 597
PspN4I GGNNCC 2 cut(s) 92, 611
PstI CTGCAG 2 cut(s) 193, 829
PsuI RGATCY 4 cut(s) 90, 149, 308, 609
PvuII CAGCTG 1 cut(s) 487
RsaI GTAC 3 cut(s) 179, 683, 722
RsaNI GTAC 3 cut(s) 178, 682, 721
SalI GTCGAC 1 cut(s) 830
SaqAI TTAA 4 cut(s) 12, 330, 429, 555
SatI GCNGC 1 cut(s) 192
Sau3AI GATC 6 cut(s) 90, 149, 308, 600, 609, 821
ScaI AGTACT 1 cut(s) 179
SchI GAGTC 4 cut(s) 27, 382, 531, 818
SfaNI GCATC 4 cut(s) 147, 377, 625, 663
SfcI CTRYAG 2 cut(s) 189, 825
SfuI TTCGAA 2 cut(s) 70, 222
SmlI CTYRAG 2 cut(s) 206, 554
SmoI CTYRAG 2 cut(s) 206, 554
Sse9I AATT 7 cut(s) 41, 73, 81, 264, 315, 572, 804
SsiI CCGC 2 cut(s) 418, 493
SspI AATATT 2 cut(s) 10, 529
StyI CCWWGG 2 cut(s) 161, 677
TaiI ACGT 1 cut(s) 291
TaqI TCGA 8 cut(s) 45, 70, 198, 222, 285, 778, 812, 831
TasI AATT 7 cut(s) 41, 73, 81, 264, 315, 572, 804
TatI WGTACW 1 cut(s) 177
TfiI GAWTC 2 cut(s) 292, 499
Tru1I TTAA 4 cut(s) 12, 330, 429, 555
Tru9I TTAA 4 cut(s) 12, 330, 429, 555
TseFI GTSAC 1 cut(s) 411
TseI GCWGC 1 cut(s) 191
Tsp45I GTSAC 1 cut(s) 411
TspDTI ATGAA 3 cut(s) 263, 267, 554
TspGWI ACGGA 1 cut(s) 830
Vha464I CTTAAG 1 cut(s) 554
XapI RAATTY 1 cut(s) 41
XmiI GTMKAC 2 cut(s) 692, 831
ZrmI AGTACT 1 cut(s) 179
Zsp2I ATGCAT 1 cut(s) 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.