Rorug03G0325100

Sieve element occlusion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000511
Physical Location & Seq
Forward (+)
35724672 .. 35724929
258 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0325100.1

Sequence Viewer

Length: 258 bp
ATGGCTGAAGAAGATCCCAAAGAAAAGGCCGACCGTGAGCGCATTTTCAAGCACTTCGACACCAATGGCGATGGCAAAATCTCATCAGATGAGCTAGGGGATGCATTGAAAGCCCTTGGATGCGTTTCACCAGAAGAAATTAAATTCATGATGGAAGAGATTGACACCGACGGCGACGGCTTCATTTCTTACGAGGAGTATATATCTTTTGCACTTGCTAACCGTGGTCTAATGAAGGACATTGCCAAGATATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SEO_C SEO_N

Protein Analysis

85

Amino Acids

9.58

Weight (kDa)

4.4

Isoelectric Point (pI)

24.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_1 PF00036 13 - 39 7.9e-09 EF hand domain
EF-hand_7 PF13499 14 - 71 7.1e-17 EF-hand domain pair
EF-hand_6 PF13405 14 - 40 5.3e-09 EF-hand domain
EF-hand_5 PF13202 15 - 33 1.7e-06 EF hand
EF-hand_8 PF13833 26 - 71 2.6e-07 EF-hand domain pair
EF-hand_1 PF00036 46 - 70 1.2e-07 EF hand domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000511)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g02370 FvH4_4g02370 FvH4_4g02370 FvH4_4g02430 FvH4_4g02430 FvH4_4g02460 FvH4_4g02460 FvH4_4g02460 FvH4_4g02480 FvH4_4g02480 FvH4_4g02480 FvH4_4g02480 FvH4_6g05920
malus_domestica MD00G1111000.v1.1 MD08G1225800.v1.1 MD13G1205700.v1.1 MD13G1205800.v1.1 MD13G1205900.v1.1 MD13G1206000.v1.1 MD16G1207500.v1.1 MD16G1207600.v1.1
prunus_persica Prupe.1G028400_v2.0.a1 Prupe.1G028500_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028900_v2.0.a1 Prupe.1G029000_v2.0.a1 Prupe.1G029100_v2.0.a1
pyrus_communis pycom08g19680 pycom08g19730 pycom16g17460 pycom16g17500
rosa_chinensis RchiOBHm_Chr4g0389751 RchiOBHm_Chr4g0389771 RchiOBHm_Chr4g0389791 RchiOBHm_Chr4g0389861 RchiOBHm_Chr4g0389881 RchiOBHm_Chr4g0389901 RchiOBHm_Chr4g0412411
rosa_laevigata RLG00000008288 RLG00000009901 RLG00000009945 RLG00000009954 RLG00000009956 RLG00000009957 RLG00000009958 RLG00000019246
rosa_multiflora Rmu_co8275125.1_g000001 Rmu_sc0002083.1_g000003 Rmu_sc0002083.1_g000025 Rmu_sc0002604.1_g000016 Rmu_sc0004336.1_g000031 Rmu_sc0010423.1_g000003
rosa_roxburghii Rroxscaffold_5G00335790 Rroxscaffold_5G00335850 Rroxscaffold_5G00335970 Rroxscaffold_5G00335990
rosa_rugosa Rorug03G0325100 Rorug03G0325300 Rorug03G0325500.1 Rorug03G0326800 Rorug03G0327000 Rorug04G0104400
rosa_samantha Rh4AG029900 Rh4AG030000 Rh4AG030100 Rh4AG030200 Rh4AG030600 Rh4AG172900 Rh4BG023200 Rh4BG023900 Rh4BG024000 Rh4BG024100 Rh4BG172200 Rh4DG023300 Rh4DG023800 Rh4DG023900 Rh4DG024000 Rh4DG151600
rosa_wichuraiana Rw1G002330 Rw1G002340 Rw4G002260 Rw4G002270 Rw4G002280 Rw4G002300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 8
AcsI RAATTY 1 cut(s) 143
AcuI CTGAAG 1 cut(s) 27
AgsI TTSAA 2 cut(s) 49, 109
AluBI AGCT 1 cut(s) 94
AluI AGCT 1 cut(s) 94
AlwI GGATC 1 cut(s) 8
AoxI GGCC 1 cut(s) 27
ApoI RAATTY 1 cut(s) 143
AspLEI GCGC 1 cut(s) 42
AsuHPI GGTGA 1 cut(s) 120
BccI CCATC 2 cut(s) 65, 145
BceAI ACGGC 2 cut(s) 187, 193
BfaI CTAG 1 cut(s) 95
BmsI GCATC 2 cut(s) 91, 110
BsaJI CCNNGG 2 cut(s) 115, 223
Bse3DI GCAATG 1 cut(s) 240
BseDI CCNNGG 2 cut(s) 115, 223
BseGI GGATG 2 cut(s) 106, 125
BseMI GCAATG 1 cut(s) 240
BseRI GAGGAG 1 cut(s) 209
Bsh1285I CGRYCG 1 cut(s) 34
BshFI GGCC 1 cut(s) 29
BsiEI CGRYCG 1 cut(s) 34
BsnI GGCC 1 cut(s) 29
Bsp143I GATC 1 cut(s) 13
BspANI GGCC 1 cut(s) 29
BspHI TCATGA 1 cut(s) 147
BspPI GGATC 1 cut(s) 8
BsrDI GCAATG 1 cut(s) 240
BssECI CCNNGG 2 cut(s) 115, 223
BssMI GATC 1 cut(s) 13
BssT1I CCWWGG 1 cut(s) 115
Bst4CI ACNGT 2 cut(s) 35, 224
Bst6I CTCTTC 1 cut(s) 150
BstDSI CCRYGG 1 cut(s) 223
BstF5I GGATG 2 cut(s) 106, 125
BstHHI GCGC 1 cut(s) 42
BstKTI GATC 1 cut(s) 16
BstMBI GATC 1 cut(s) 13
BstMCI CGRYCG 1 cut(s) 34
BstMWI GCNNNNNNNGC 1 cut(s) 110
BstX2I RGATCY 1 cut(s) 13
BstYI RGATCY 1 cut(s) 13
BsuRI GGCC 1 cut(s) 29
BtgI CCRYGG 1 cut(s) 223
BtgZI GCGATG 1 cut(s) 84
BtsCI GGATG 2 cut(s) 106, 125
CciI TCATGA 1 cut(s) 147
CfoI GCGC 1 cut(s) 42
CviAII CATG 1 cut(s) 148
CviJI RGCY 5 cut(s) 5, 29, 94, 113, 180
CviKI_1 RGCY 5 cut(s) 5, 29, 94, 113, 180
DpnI GATC 1 cut(s) 15
DpnII GATC 1 cut(s) 13
Eam1104I CTCTTC 1 cut(s) 150
EarI CTCTTC 1 cut(s) 150
Eco130I CCWWGG 1 cut(s) 115
Eco57I CTGAAG 1 cut(s) 27
EcoT14I CCWWGG 1 cut(s) 115
EcoT22I ATGCAT 1 cut(s) 106
ErhI CCWWGG 1 cut(s) 115
FaeI CATG 1 cut(s) 151
FaiI YATR 3 cut(s) 149, 201, 203
FatI CATG 1 cut(s) 147
FokI GGATG 2 cut(s) 113, 132
FspBI CTAG 1 cut(s) 95
GlaI GCGC 1 cut(s) 41
HaeIII GGCC 1 cut(s) 29
HhaI GCGC 1 cut(s) 42
Hin1II CATG 1 cut(s) 151
Hin6I GCGC 1 cut(s) 40
HinP1I GCGC 1 cut(s) 40
HphI GGTGA 1 cut(s) 120
Hpy188I TCNGA 1 cut(s) 88
Hpy188III TCNNGA 1 cut(s) 148
Hpy99I CGWCG 2 cut(s) 173, 179
HpyAV CCTTC 1 cut(s) 229
HpyCH4III ACNGT 2 cut(s) 35, 224
HpyCH4V TGCA 2 cut(s) 104, 212
HpyF10VI GCNNNNNNNGC 1 cut(s) 110
Hsp92II CATG 1 cut(s) 151
HspAI GCGC 1 cut(s) 40
Kzo9I GATC 1 cut(s) 13
LpnPI CCDG 1 cut(s) 144
LweI GCATC 2 cut(s) 91, 110
MaeI CTAG 1 cut(s) 95
MalI GATC 1 cut(s) 15
MboI GATC 1 cut(s) 13
MboII GAAGA 4 cut(s) 20, 23, 146, 167
MflI RGATCY 1 cut(s) 13
MluCI AATT 2 cut(s) 138, 143
MnlI CCTC 1 cut(s) 187
Mph1103I ATGCAT 1 cut(s) 106
MseI TTAA 2 cut(s) 141, 256
MwoI GCNNNNNNNGC 1 cut(s) 110
NdeII GATC 1 cut(s) 13
NlaIII CATG 1 cut(s) 151
NsiI ATGCAT 1 cut(s) 106
PagI TCATGA 1 cut(s) 147
PsuI RGATCY 1 cut(s) 13
SaqAI TTAA 2 cut(s) 141, 256
Sau3AI GATC 1 cut(s) 13
SetI ASST 1 cut(s) 96
SfaNI GCATC 2 cut(s) 91, 110
SgeI CNNG 9 cut(s) 47, 61, 107, 128, 143, 160, 205, 227, 236
Sse9I AATT 2 cut(s) 138, 143
SspMI CTAG 1 cut(s) 95
StyI CCWWGG 1 cut(s) 115
TaaI ACNGT 2 cut(s) 35, 224
TaqI TCGA 1 cut(s) 57
TasI AATT 2 cut(s) 138, 143
Tru1I TTAA 2 cut(s) 141, 256
Tru9I TTAA 2 cut(s) 141, 256
TspDTI ATGAA 3 cut(s) 136, 172, 248
XapI RAATTY 1 cut(s) 143
XspI CTAG 1 cut(s) 95
Zsp2I ATGCAT 1 cut(s) 106
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.