Rh4BG172200

Sieve element occlusion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000511
Physical Location & Seq
Forward (+)
30792313 .. 30795288
2976 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG172200.1

Sequence Viewer

Length: 2016 bp
ATGCTAGGCCTAGCATTCAATGTGGCTACCAAGGTAGCCGCAGCAGTCCACCCTACTCACAAAACCATTGATGGGGAGTTTAGCCTGTTTGCCATGTCTGACAAGCAGATCTTGCAGGAGATTTACACGACTCATGTCCATGCTGATGAGACCTTGGATGATGATTCTCTCTTCATCATTGTTGAGAATATCCTCAAGCAGGCAACCCAAAATGTTGATGAAATAGTGCAGGGTATCCAAGTGCATGTGGACAACATAGAGGAGAAAAACCCCAAAGCCAGCTTCAGTGTACCATTGTGCACGCTAAAGGGAATCTCATGCGAGATGCAATGCAAGCCTCCTGGTGACGAAATCGCCCACAACACAACAGTGGCAATACTCAACAAGCTCTCAAAGTATTCATGGGAAGCAAAGGCATCACTGACCCTAGCTGCTTTTGCTATGGAATATGGTGAATTTTGGCTCCTTGCGCAGCTTCGCGAATCAAACCACCTTGCCAAATCAATTGCAATCCTCAAACGAGTCCCTGTTCTCCTCAAGCCAATGGAGCTGCAAAAGCGCAGGCAATCAATTCTTCAGCTTAACAATCTGATCAAGGCCACACTACAATATTCCCGTTATCTCCCAAAAGAATTCCCCGGTTTGTCGATAGCATCGGAACATATTCCAGTTGATGTCTATTGGGCTATCATCACTGTTGTTGCTTGTGCCACTAAGATCACTATACTAACTAGTGATGAGGACAAGGAGTTTGATCTAGCCCCCTATTCTCAAAAAATCCACTACGTCCTTAACAAGCTAACGATTCAGCTGAGAGGTTGCAAAACATATATTGAGGAGGAAGAGACTTACATAAGGATCAAGAAATTGTTCCAAACGCCTACTGAAATTATGGAGGTTTTCAAGGGATTAATATTTACAAAGGATAATGTCCAGCCACTCGTCGATGGTTCAACTAAGCAAACGGTTAACATCGACATCCTAAGGAGGAAGAATGTGCTATTGTTCATTTCCAGTCTAGACATTACGGATGACGACATTTCGATTCTAAAACCGATCCACGAGTTCACAAAGAAAGACAATCAGTATAACATCGTCTGGATACCAATTGTGGAGCAATGGACTGATGACCTGAGAAAGAAGTTCGATATCCTGAAGAACAAGATGCCGTGGTTTACGGTGCAGTACCCTGGACCTATAGCAGGCATCAAGTACATCAAGAACGACTGGAACTTCAAGGGTAAGCCTACGATGGTGGTGATGAACTCACATGGAAAGGTCGAACACCCTAACGCTGTCCACATGGTTCGGGTGTGGGGAGTAAAGGCCTTCCCTTTCACAGAGACAAGAGAAGCAGAACTGTCACATTCTGATGGAGAAAAATGGGTTTCCTCCGTTGTTAACGGAATTCACCCATCTCTGCCCACTTGGATCAAAGAAAATAAGTACATCTTCTTCTATGGCGGTAAAGACAACGTATGGATTCAGAATTTTAAAAATAAAGCAACTGCCCTTGCAAAAGATCCAATCTTCAAGGAAGCAAAGATAGAGTTGGTTTGCGTTGGAAAAGACAGCAAAGGAGAAGATGACCATGGCGTTCTAGGCCGCTTCTGGACCGGTGTAGAAAGCCTGTTCTTAACCACAGGCCAATGGCAGGTCGACTCGGTCAGTGAAGAAATCCAGAAGCTTCTCTCTTACAAAAACGAAAGTGGATGGGCAGTGCTTTGCAAAGGGTCGACTGTGTTGGTCACGGCTCATGGTGTCTCGGTGTTGAAGGTGGTAGAGGACTTTGAGAAATGGAAGGACCCTGTCAAGGAGAACGGGTTTGAGTTTTGTTTCACAACCTACTATGGGACGATTATTCAAGGTAGCCGTCAACCTTGCTGCCGTGTGGACATTCCGGGGTCTAACGGAAAGGTTCCAGATTCGATGAAGTGCCCTGATTGCCACCGTAGTATGGAGACTATTATCAGTTACAAGTGCTGCCACACTGATGGTCCTATTGCACATCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SEO_C SEO_N

Protein Analysis

671

Amino Acids

76.16

Weight (kDa)

6.61

Isoelectric Point (pI)

35.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SEO_N PF14576 33 - 294 2.1e-73 Sieve element occlusion N-terminus
SEO_C PF14577 464 - 529 7.3e-12 Sieve element occlusion C-terminus
SEO_C PF14577 537 - 663 2.5e-30 Sieve element occlusion C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000511)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g02370 FvH4_4g02370 FvH4_4g02370 FvH4_4g02430 FvH4_4g02430 FvH4_4g02460 FvH4_4g02460 FvH4_4g02460 FvH4_4g02480 FvH4_4g02480 FvH4_4g02480 FvH4_4g02480 FvH4_6g05920
malus_domestica MD00G1111000.v1.1 MD08G1225800.v1.1 MD13G1205700.v1.1 MD13G1205800.v1.1 MD13G1205900.v1.1 MD13G1206000.v1.1 MD16G1207500.v1.1 MD16G1207600.v1.1
prunus_persica Prupe.1G028400_v2.0.a1 Prupe.1G028500_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028900_v2.0.a1 Prupe.1G029000_v2.0.a1 Prupe.1G029100_v2.0.a1
pyrus_communis pycom08g19680 pycom08g19730 pycom16g17460 pycom16g17500
rosa_chinensis RchiOBHm_Chr4g0389751 RchiOBHm_Chr4g0389771 RchiOBHm_Chr4g0389791 RchiOBHm_Chr4g0389861 RchiOBHm_Chr4g0389881 RchiOBHm_Chr4g0389901 RchiOBHm_Chr4g0412411
rosa_laevigata RLG00000008288 RLG00000009901 RLG00000009945 RLG00000009954 RLG00000009956 RLG00000009957 RLG00000009958 RLG00000019246
rosa_multiflora Rmu_co8275125.1_g000001 Rmu_sc0002083.1_g000003 Rmu_sc0002083.1_g000025 Rmu_sc0002604.1_g000016 Rmu_sc0004336.1_g000031 Rmu_sc0010423.1_g000003
rosa_roxburghii Rroxscaffold_5G00335790 Rroxscaffold_5G00335850 Rroxscaffold_5G00335970 Rroxscaffold_5G00335990
rosa_rugosa Rorug03G0325100 Rorug03G0325300 Rorug03G0325500.1 Rorug03G0326800 Rorug03G0327000 Rorug04G0104400
rosa_samantha Rh4AG029900 Rh4AG030000 Rh4AG030100 Rh4AG030200 Rh4AG030600 Rh4AG172900 Rh4BG023200 Rh4BG023900 Rh4BG024000 Rh4BG024100 Rh4BG172200 Rh4DG023300 Rh4DG023800 Rh4DG023900 Rh4DG024000 Rh4DG151600
rosa_wichuraiana Rw1G002330 Rw1G002340 Rw4G002260 Rw4G002270 Rw4G002280 Rw4G002300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 471
Acc36I ACCTGC 1 cut(s) 1645
AccI GTMKAC 2 cut(s) 1659, 1736
AccII CGCG 1 cut(s) 480
AciI CCGC 3 cut(s) 39, 1464, 1606
AclWI GGATC 4 cut(s) 866, 1051, 1439, 1517
AcsI RAATTY 4 cut(s) 455, 632, 1407, 1489
AcuI CTGAAG 3 cut(s) 268, 560, 1175
AfaI GTAC 4 cut(s) 291, 1187, 1214, 1448
AfiI CCNNNNNNNGG 7 cut(s) 72, 199, 1202, 1654, 1813, 1851, 1957
AgeI ACCGGT 1 cut(s) 1616
AgsI TTSAA 7 cut(s) 19, 904, 954, 1237, 1534, 1774, 1865
AhlI ACTAGT 1 cut(s) 731
AjnI CCWGG 2 cut(s) 340, 1189
AjuI GAANNNNNNNTTGG 2 cut(s) 266, 298
AleI CACNNNNGTG 1 cut(s) 368
AluBI AGCT 9 cut(s) 282, 388, 431, 475, 550, 580, 799, 811, 1687
AluI AGCT 9 cut(s) 282, 388, 431, 475, 550, 580, 799, 811, 1687
Alw21I GWGCWC 1 cut(s) 302
Alw26I GTCTC 5 cut(s) 143, 839, 1337, 1768, 1955
Alw44I GTGCAC 1 cut(s) 298
AlwI GGATC 4 cut(s) 866, 1051, 1439, 1517
AoxI GGCC 5 cut(s) 7, 597, 1326, 1603, 1645
ApaLI GTGCAC 1 cut(s) 298
ApeKI GCWGC 6 cut(s) 41, 431, 472, 550, 1884, 1983
ApoI RAATTY 4 cut(s) 455, 632, 1407, 1489
ArsI GACNNNNNNTTYG 2 cut(s) 734, 766
AseI ATTAAT 1 cut(s) 911
AsiGI ACCGGT 1 cut(s) 1616
Asp700I GAANNNNTTC 2 cut(s) 663, 869
AspLEI GCGC 2 cut(s) 472, 561
AspS9I GGNCC 4 cut(s) 1193, 1614, 1804, 1997
AsuC2I CCSGG 2 cut(s) 639, 1902
AsuHPI GGTGA 4 cut(s) 356, 464, 1270, 1403
AvaII GGWCC 4 cut(s) 1193, 1614, 1804, 1997
AxyI CCTNAGG 1 cut(s) 983
BaeGI GKGCMC 2 cut(s) 302, 1940
BarI GAAGNNNNNNTAC 2 cut(s) 1439, 1471
BauI CACGAG 1 cut(s) 1061
Bbv12I GWGCWC 1 cut(s) 302
BbvI GCAGC 6 cut(s) 53, 418, 484, 537, 1871, 1970
BccI CCATC 7 cut(s) 65, 941, 1246, 1367, 1423, 1707, 1988
BceAI ACGGC 4 cut(s) 1153, 1767, 1857, 1872
BciT130I CCWGG 2 cut(s) 342, 1191
BciVI GTATCC 2 cut(s) 245, 1095
BclI TGATCA 1 cut(s) 591
BcnI CCSGG 2 cut(s) 639, 1902
BcoDI GTCTC 5 cut(s) 143, 839, 1337, 1768, 1955
BcuI ACTAGT 1 cut(s) 731
BfaI CTAG 7 cut(s) 5, 11, 428, 732, 758, 1019, 1601
BfmI CTRYAG 1 cut(s) 1197
BfuAI ACCTGC 1 cut(s) 1645
BfuI GTATCC 2 cut(s) 245, 1095
BglII AGATCT 1 cut(s) 108
BisI GCNGC 8 cut(s) 39, 42, 432, 473, 551, 1606, 1885, 1984
BlsI GCNGC 8 cut(s) 40, 43, 433, 474, 552, 1607, 1886, 1985
Bme1390I CCNGG 4 cut(s) 342, 639, 1191, 1902
Bme18I GGWCC 4 cut(s) 1193, 1614, 1804, 1997
BmgT120I GGNCC 4 cut(s) 1193, 1614, 1804, 1997
BmiI GGNNCC 3 cut(s) 464, 1806, 1920
BmrFI CCNGG 4 cut(s) 342, 639, 1191, 1902
BmsI GCATC 5 cut(s) 315, 425, 662, 1155, 1215
BoxI GACNNNNGTC 1 cut(s) 134
BpuEI CTTGAG 2 cut(s) 179, 521
BpuMI CCSGG 2 cut(s) 639, 1902
BsaI GGTCTC 1 cut(s) 143
BsaJI CCNNGG 7 cut(s) 30, 153, 637, 1169, 1189, 1591, 1901
BsaWI WCCGGW 1 cut(s) 1616
BsaXI ACNNNNNCTCC 2 cut(s) 1808, 1838
Bsc4I CCNNNNNNNGG 7 cut(s) 72, 199, 1202, 1654, 1813, 1851, 1957
Bse118I RCCGGY 1 cut(s) 1616
Bse1I ACTGG 3 cut(s) 668, 1014, 1232
Bse21I CCTNAGG 1 cut(s) 983
Bse3DI GCAATG 2 cut(s) 335, 1124
BseBI CCWGG 2 cut(s) 342, 1191
BseDI CCNNGG 7 cut(s) 30, 153, 637, 1169, 1189, 1591, 1901
BseGI GGATG 4 cut(s) 163, 978, 1036, 1718
BseLI CCNNNNNNNGG 7 cut(s) 72, 199, 1202, 1654, 1813, 1851, 1957
BseMI GCAATG 2 cut(s) 335, 1124
BseMII CTCAG 2 cut(s) 803, 1124
BseNI ACTGG 3 cut(s) 668, 1014, 1232
BseRI GAGGAG 3 cut(s) 275, 524, 851
BseSI GKGCMC 2 cut(s) 302, 1940
BseXI GCAGC 6 cut(s) 53, 418, 484, 537, 1871, 1970
BsgI GTGCAG 2 cut(s) 248, 1202
Bsh1236I CGCG 1 cut(s) 480
BshFI GGCC 5 cut(s) 9, 599, 1328, 1605, 1647
BshTI ACCGGT 1 cut(s) 1616
BsiHKAI GWGCWC 1 cut(s) 302
BsiSI CCGG 3 cut(s) 639, 1617, 1901
BslFI GGGAC 2 cut(s) 509, 1867
BslI CCNNNNNNNGG 7 cut(s) 72, 199, 1202, 1654, 1813, 1851, 1957
BsmAI GTCTC 5 cut(s) 143, 839, 1337, 1768, 1955
BsmFI GGGAC 2 cut(s) 509, 1867
BsmI GAATGC 1 cut(s) 14
BsnI GGCC 5 cut(s) 9, 599, 1328, 1605, 1647
Bso31I GGTCTC 1 cut(s) 143
Bsp1286I GDGCHC 2 cut(s) 302, 1940
Bsp143I GATC 8 cut(s) 108, 591, 717, 754, 858, 1056, 1431, 1522
Bsp19I CCATGG 1 cut(s) 1591
Bsp68I TCGCGA 1 cut(s) 480
BspACI CCGC 3 cut(s) 39, 1464, 1606
BspANI GGCC 5 cut(s) 9, 599, 1328, 1605, 1647
BspCNI CTCAG 2 cut(s) 804, 1125
BspFNI CGCG 1 cut(s) 480
BspLI GGNNCC 3 cut(s) 464, 1806, 1920
BspMI ACCTGC 1 cut(s) 1645
BspPI GGATC 4 cut(s) 866, 1051, 1439, 1517
BspTNI GGTCTC 1 cut(s) 143
BsrDI GCAATG 2 cut(s) 335, 1124
BsrFI RCCGGY 1 cut(s) 1616
BsrI ACTGG 3 cut(s) 668, 1014, 1232
BssAI RCCGGY 1 cut(s) 1616
BssECI CCNNGG 7 cut(s) 30, 153, 637, 1169, 1189, 1591, 1901
BssMI GATC 8 cut(s) 108, 591, 717, 754, 858, 1056, 1431, 1522
BssSI CACGAG 1 cut(s) 1061
BssT1I CCWWGG 3 cut(s) 30, 153, 1591
Bst2BI CACGAG 1 cut(s) 1061
Bst2UI CCWGG 2 cut(s) 342, 1191
Bst4CI ACNGT 7 cut(s) 370, 697, 967, 1180, 1362, 1741, 1952
Bst6I CTCTTC 2 cut(s) 176, 837
BstAPI GCANNNNNTGC 1 cut(s) 112
BstC8I GCNNGC 6 cut(s) 201, 280, 302, 335, 563, 1204
BstDEI CTNAG 5 cut(s) 714, 812, 957, 983, 1133
BstDSI CCRYGG 2 cut(s) 1169, 1591
BstENI CCTNNNNNAGG 2 cut(s) 197, 1200
BstF5I GGATG 4 cut(s) 163, 978, 1036, 1718
BstFNI CGCG 1 cut(s) 480
BstHHI GCGC 2 cut(s) 472, 561
BstKTI GATC 8 cut(s) 111, 594, 720, 757, 861, 1059, 1434, 1525
BstMAI GTCTC 5 cut(s) 143, 839, 1337, 1768, 1955
BstMBI GATC 8 cut(s) 108, 591, 717, 754, 858, 1056, 1431, 1522
BstMWI GCNNNNNNNGC 8 cut(s) 112, 334, 437, 469, 547, 556, 1602, 1944
BstNI CCWGG 2 cut(s) 342, 1191
BstNSI RCATGY 1 cut(s) 248
BstPAI GACNNNNGTC 1 cut(s) 134
BstSCI CCNGG 4 cut(s) 340, 637, 1189, 1900
BstSFI CTRYAG 1 cut(s) 1197
BstSLI GKGCMC 2 cut(s) 302, 1940
BstUI CGCG 1 cut(s) 480
BstV1I GCAGC 6 cut(s) 53, 418, 484, 537, 1871, 1970
BstX2I RGATCY 2 cut(s) 108, 1522
BstXI CCANNNNNNTGG 1 cut(s) 1994
BstYI RGATCY 2 cut(s) 108, 1522
Bsu36I CCTNAGG 1 cut(s) 983
BsuI GTATCC 2 cut(s) 245, 1095
BsuRI GGCC 5 cut(s) 9, 599, 1328, 1605, 1647
BtgI CCRYGG 2 cut(s) 1169, 1591
BtsCI GGATG 4 cut(s) 163, 978, 1036, 1718
BtsI GCAGTG 1 cut(s) 1725
BtsIMutI CAGTG 7 cut(s) 292, 375, 419, 693, 1675, 1725, 1989
BtuMI TCGCGA 1 cut(s) 480
BveI ACCTGC 1 cut(s) 1645
Cac8I GCNNGC 6 cut(s) 201, 280, 302, 335, 563, 1204
CfoI GCGC 2 cut(s) 472, 561
Cfr10I RCCGGY 1 cut(s) 1616
Cfr13I GGNCC 4 cut(s) 1193, 1614, 1804, 1997
Csp6I GTAC 4 cut(s) 290, 1186, 1213, 1447
CspAI ACCGGT 1 cut(s) 1616
CviQI GTAC 4 cut(s) 290, 1186, 1213, 1447
DdeI CTNAG 5 cut(s) 714, 812, 957, 983, 1133
DpnI GATC 8 cut(s) 110, 593, 719, 756, 860, 1058, 1433, 1524
DpnII GATC 8 cut(s) 108, 591, 717, 754, 858, 1056, 1431, 1522
DraI TTTAAA 1 cut(s) 1495
Eam1104I CTCTTC 2 cut(s) 176, 837
EarI CTCTTC 2 cut(s) 176, 837
Eco130I CCWWGG 3 cut(s) 30, 153, 1591
Eco147I AGGCCT 2 cut(s) 9, 1328
Eco31I GGTCTC 1 cut(s) 143
Eco32I GATATC 1 cut(s) 1150
Eco47I GGWCC 4 cut(s) 1193, 1614, 1804, 1997
Eco57I CTGAAG 3 cut(s) 268, 560, 1175
Eco81I CCTNAGG 1 cut(s) 983
EcoNI CCTNNNNNAGG 2 cut(s) 197, 1200
EcoO109I RGGNCCY 1 cut(s) 1804
EcoRI GAATTC 2 cut(s) 632, 1407
EcoRII CCWGG 2 cut(s) 340, 1189
EcoRV GATATC 1 cut(s) 1150
EcoT14I CCWWGG 3 cut(s) 30, 153, 1591
ErhI CCWWGG 3 cut(s) 30, 153, 1591
FalI AAGNNNNNCTT 4 cut(s) 95, 127, 1436, 1468
FaqI GGGAC 2 cut(s) 509, 1867
FbaI TGATCA 1 cut(s) 591
FblI GTMKAC 2 cut(s) 1659, 1736
Fnu4HI GCNGC 8 cut(s) 39, 42, 432, 473, 551, 1606, 1885, 1984
FokI GGATG 4 cut(s) 170, 965, 1043, 1725
Fsp4HI GCNGC 8 cut(s) 39, 42, 432, 473, 551, 1606, 1885, 1984
FspBI CTAG 7 cut(s) 5, 11, 428, 732, 758, 1019, 1601
FspI TGCGCA 1 cut(s) 471
GlaI GCGC 2 cut(s) 471, 560
GluI GCNGC 8 cut(s) 39, 42, 432, 473, 551, 1606, 1885, 1984
HaeIII GGCC 5 cut(s) 9, 599, 1328, 1605, 1647
HapII CCGG 3 cut(s) 639, 1617, 1901
HhaI GCGC 2 cut(s) 472, 561
Hin6I GCGC 2 cut(s) 470, 559
HinP1I GCGC 2 cut(s) 470, 559
HincII GTYRAC 5 cut(s) 970, 1402, 1660, 1737, 1877
HindII GTYRAC 5 cut(s) 970, 1402, 1660, 1737, 1877
HindIII AAGCTT 1 cut(s) 1685
HpaI GTTAAC 2 cut(s) 970, 1402
HpaII CCGG 3 cut(s) 639, 1617, 1901
HphI GGTGA 4 cut(s) 356, 464, 1270, 1403
Hpy188I TCNGA 5 cut(s) 100, 591, 658, 1372, 1488
Hpy188III TCNNGA 9 cut(s) 479, 862, 1019, 1099, 1153, 1219, 1612, 1681, 1922
Hpy99I CGWCG 1 cut(s) 947
HpyAV CCTTC 3 cut(s) 1339, 1768, 1795
HpyCH4III ACNGT 7 cut(s) 370, 697, 967, 1180, 1362, 1741, 1952
HpyCH4IV ACGT 2 cut(s) 786, 1476
HpyF10VI GCNNNNNNNGC 8 cut(s) 112, 334, 437, 469, 547, 556, 1602, 1944
HpyF3I CTNAG 5 cut(s) 714, 812, 957, 983, 1133
HpySE526I ACGT 2 cut(s) 786, 1476
HspAI GCGC 2 cut(s) 470, 559
Ksp22I TGATCA 1 cut(s) 591
KspAI GTTAAC 2 cut(s) 970, 1402
Kzo9I GATC 8 cut(s) 108, 591, 717, 754, 858, 1056, 1431, 1522
LmnI GCTCC 3 cut(s) 468, 547, 1114
Lsp1109I GCAGC 6 cut(s) 53, 418, 484, 537, 1871, 1970
LweI GCATC 5 cut(s) 315, 425, 662, 1155, 1215
MaeI CTAG 7 cut(s) 5, 11, 428, 732, 758, 1019, 1601
MaeII ACGT 2 cut(s) 786, 1476
MaeIII GTNAC 4 cut(s) 344, 1362, 1747, 1973
MalI GATC 8 cut(s) 110, 593, 719, 756, 860, 1058, 1433, 1524
MboI GATC 8 cut(s) 108, 591, 717, 754, 858, 1056, 1431, 1522
MfeI CAATTG 2 cut(s) 504, 1107
MflI RGATCY 2 cut(s) 108, 1522
MhlI GDGCHC 2 cut(s) 302, 1940
MluCI AATT 9 cut(s) 455, 504, 570, 632, 866, 888, 1107, 1407, 1489
MlyI GAGTC 3 cut(s) 124, 531, 1655
MmeI TCCRAC 1 cut(s) 1543
MroXI GAANNNNTTC 2 cut(s) 663, 869
MseI TTAA 8 cut(s) 582, 792, 911, 969, 1401, 1494, 1637, 2014
MslI CAYNNNNRTG 5 cut(s) 138, 144, 368, 1371, 1992
MspA1I CMGCKG 1 cut(s) 811
MspI CCGG 3 cut(s) 639, 1617, 1901
MspR9I CCNGG 4 cut(s) 342, 639, 1191, 1902
MunI CAATTG 2 cut(s) 504, 1107
Mva1269I GAATGC 1 cut(s) 14
MvaI CCWGG 2 cut(s) 342, 1191
MvnI CGCG 1 cut(s) 480
MwoI GCNNNNNNNGC 8 cut(s) 112, 334, 437, 469, 547, 556, 1602, 1944
NciI CCSGG 2 cut(s) 639, 1902
NcoI CCATGG 1 cut(s) 1591
NdeII GATC 8 cut(s) 108, 591, 717, 754, 858, 1056, 1431, 1522
NlaIV GGNNCC 3 cut(s) 464, 1806, 1920
NmuCI GTSAC 3 cut(s) 344, 1362, 1747
NruI TCGCGA 1 cut(s) 480
NsbI TGCGCA 1 cut(s) 471
NspI RCATGY 1 cut(s) 248
OliI CACNNNNGTG 1 cut(s) 368
PceI AGGCCT 2 cut(s) 9, 1328
PctI GAATGC 1 cut(s) 14
PdmI GAANNNNTTC 2 cut(s) 663, 869
PfeI GAWTC 7 cut(s) 164, 312, 482, 805, 1045, 1483, 1925
PflFI GACNNNGTC 2 cut(s) 1664, 1808
PinAI ACCGGT 1 cut(s) 1616
PkrI GCNGC 8 cut(s) 40, 43, 433, 474, 552, 1607, 1886, 1985
PleI GAGTC 3 cut(s) 124, 530, 1655
PpsI GAGTC 3 cut(s) 124, 530, 1655
PpuMI RGGWCCY 1 cut(s) 1804
PshAI GACNNNNGTC 1 cut(s) 134
PshBI ATTAAT 1 cut(s) 911
Psp5II RGGWCCY 1 cut(s) 1804
Psp6I CCWGG 2 cut(s) 340, 1189
PspGI CCWGG 2 cut(s) 340, 1189
PspN4I GGNNCC 3 cut(s) 464, 1806, 1920
PspPI GGNCC 4 cut(s) 1193, 1614, 1804, 1997
PspPPI RGGWCCY 1 cut(s) 1804
PsuI RGATCY 2 cut(s) 108, 1522
PsyI GACNNNGTC 2 cut(s) 1664, 1808
PvuII CAGCTG 1 cut(s) 811
RruI TCGCGA 1 cut(s) 480
RsaI GTAC 4 cut(s) 291, 1187, 1214, 1448
RsaNI GTAC 4 cut(s) 290, 1186, 1213, 1447
RseI CAYNNNNRTG 5 cut(s) 138, 144, 368, 1371, 1992
SalI GTCGAC 2 cut(s) 1658, 1735
SaqAI TTAA 8 cut(s) 582, 792, 911, 969, 1401, 1494, 1637, 2014
SatI GCNGC 8 cut(s) 39, 42, 432, 473, 551, 1606, 1885, 1984
Sau3AI GATC 8 cut(s) 108, 591, 717, 754, 858, 1056, 1431, 1522
Sau96I GGNCC 4 cut(s) 1193, 1614, 1804, 1997
SchI GAGTC 3 cut(s) 124, 531, 1655
ScrFI CCNGG 4 cut(s) 342, 639, 1191, 1902
SduI GDGCHC 2 cut(s) 302, 1940
SfaNI GCATC 5 cut(s) 315, 425, 662, 1155, 1215
SfcI CTRYAG 1 cut(s) 1197
SinI GGWCC 4 cut(s) 1193, 1614, 1804, 1997
SmiMI CAYNNNNRTG 5 cut(s) 138, 144, 368, 1371, 1992
SmlI CTYRAG 2 cut(s) 194, 536
SmoI CTYRAG 2 cut(s) 194, 536
SpeI ACTAGT 1 cut(s) 731
Sse9I AATT 9 cut(s) 455, 504, 570, 632, 866, 888, 1107, 1407, 1489
SseBI AGGCCT 2 cut(s) 9, 1328
SsiI CCGC 3 cut(s) 39, 1464, 1606
SspI AATATT 2 cut(s) 611, 915
SspMI CTAG 7 cut(s) 5, 11, 428, 732, 758, 1019, 1601
StuI AGGCCT 2 cut(s) 9, 1328
StyD4I CCNGG 4 cut(s) 340, 637, 1189, 1900
StyI CCWWGG 3 cut(s) 30, 153, 1591
TaaI ACNGT 7 cut(s) 370, 697, 967, 1180, 1362, 1741, 1952
TaiI ACGT 2 cut(s) 789, 1479
TaqI TCGA 9 cut(s) 647, 945, 975, 1043, 1146, 1281, 1659, 1736, 1928
TaqII GACCGA 1 cut(s) 1654
TasI AATT 9 cut(s) 455, 504, 570, 632, 866, 888, 1107, 1407, 1489
TatI WGTACW 2 cut(s) 1212, 1446
TauI GCSGC 2 cut(s) 41, 1608
TfiI GAWTC 7 cut(s) 164, 312, 482, 805, 1045, 1483, 1925
Tru1I TTAA 8 cut(s) 582, 792, 911, 969, 1401, 1494, 1637, 2014
Tru9I TTAA 8 cut(s) 582, 792, 911, 969, 1401, 1494, 1637, 2014
TscAI CASTG 7 cut(s) 292, 375, 426, 700, 1675, 1725, 1996
TseFI GTSAC 3 cut(s) 344, 1362, 1747
TseI GCWGC 6 cut(s) 41, 431, 472, 550, 1884, 1983
Tsp45I GTSAC 3 cut(s) 344, 1362, 1747
TspDTI ATGAA 6 cut(s) 163, 234, 390, 997, 1277, 1946
TspGWI ACGGA 4 cut(s) 1043, 1384, 1419, 1926
TspRI CASTG 7 cut(s) 292, 375, 426, 700, 1675, 1725, 1996
Tth111I GACNNNGTC 2 cut(s) 1664, 1808
VneI GTGCAC 1 cut(s) 298
VpaK11BI GGWCC 4 cut(s) 1193, 1614, 1804, 1997
VspI ATTAAT 1 cut(s) 911
XagI CCTNNNNNAGG 2 cut(s) 197, 1200
XapI RAATTY 4 cut(s) 455, 632, 1407, 1489
XbaI TCTAGA 1 cut(s) 1018
XceI RCATGY 1 cut(s) 248
XmiI GTMKAC 2 cut(s) 1659, 1736
XmnI GAANNNNTTC 2 cut(s) 663, 869
XspI CTAG 7 cut(s) 5, 11, 428, 732, 758, 1019, 1601
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.