Rw4G002260

Sieve element occlusion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000511
Physical Location & Seq
Forward (+)
4163564 .. 4165071
1508 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G002260.1

Sequence Viewer

Length: 1014 bp
ATGCTAGGCCTAGCGCACAATGTTGCTACCAAGGTAGCCACAGTAGTCCACACTACTCAGAAAACCATTGCAGGGGAGCTTAGTCTGTTTGCCATGTCTGACAAGAAGATCTTGGAGGAGATTTACACGACTCATGTCCATGCTGATGAATCCTTCGATGACGATTCTCTATTCGTCATTGTTGAGAACATCCTCAAGCGTGCAACCCAAAATGTTGATAAAATAGTGCAGGGTACTCAAGTGCATGTGGACAACATACAGGAGAAAACCCCCAAAGCCAGTTTCAGCGTGCCATTGTGCACGCTAAAGGGAATCTCTTGCGAGATGCAATGCAAGCCTCCTGGTGACGAAATCGCCCACAACACAACAGTGGCAATACTCAACAAGCTGTCAGAGTATTCATGGGAAGCAAAGGCATCACTGACCCTAGCAGCTTTTGCTATGGAATATGGTGAATTCTGGCTCCTGGCGCAGCTTCGCGAATCAGACCACCTTGCCAAATCAATTGCAATCCTCAAACGAGTCCCTGTTCTCCTCAAGCCGTCAGAACTGCACAAGCGCAGTCAATCAATTCTTGAGCTTAACAATCTGATCAAGGCCATACTGCAAGTGATCGAATGCATTGACCAGTTCAATAAGTATTCCACTTATGACCCAAAAGATGTCCCCAATTTGTCTATAGCATTGGATCATATTCCGGTTAATGTCTATTGGGTTATCATCACTGTTGTTGCTTGTGCCACTAAGATCACTATACTCACTAGTGATGGGGACAAGGATTATGATCTGGCCCCTTATTCTCAAAAAATCCACCATGTCCTTAACAAGCTAACGATTCAGCTAAGAGTTTGCAGGAAACAAATTGAGGAGGCAGAGACTTACAGAAGGATCCTGAAAGTGACCCGAATCCCTACTGAAATTATGGAGGTTTTCAAGGCATTAATATTTACAAAGGATAATGTCCAGCCACTCATCGATGGTTCAACTAAGCAAACGGTTTGCTCATATCTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SEO_C SEO_N

Protein Analysis

337

Amino Acids

37.93

Weight (kDa)

6.42

Isoelectric Point (pI)

45.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SEO_N PF14576 33 - 302 9.1e-85 Sieve element occlusion N-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000511)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g02370 FvH4_4g02370 FvH4_4g02370 FvH4_4g02430 FvH4_4g02430 FvH4_4g02460 FvH4_4g02460 FvH4_4g02460 FvH4_4g02480 FvH4_4g02480 FvH4_4g02480 FvH4_4g02480 FvH4_6g05920
malus_domestica MD00G1111000.v1.1 MD08G1225800.v1.1 MD13G1205700.v1.1 MD13G1205800.v1.1 MD13G1205900.v1.1 MD13G1206000.v1.1 MD16G1207500.v1.1 MD16G1207600.v1.1
prunus_persica Prupe.1G028400_v2.0.a1 Prupe.1G028500_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028900_v2.0.a1 Prupe.1G029000_v2.0.a1 Prupe.1G029100_v2.0.a1
pyrus_communis pycom08g19680 pycom08g19730 pycom16g17460 pycom16g17500
rosa_chinensis RchiOBHm_Chr4g0389751 RchiOBHm_Chr4g0389771 RchiOBHm_Chr4g0389791 RchiOBHm_Chr4g0389861 RchiOBHm_Chr4g0389881 RchiOBHm_Chr4g0389901 RchiOBHm_Chr4g0412411
rosa_laevigata RLG00000008288 RLG00000009901 RLG00000009945 RLG00000009954 RLG00000009956 RLG00000009957 RLG00000009958 RLG00000019246
rosa_multiflora Rmu_co8275125.1_g000001 Rmu_sc0002083.1_g000003 Rmu_sc0002083.1_g000025 Rmu_sc0002604.1_g000016 Rmu_sc0004336.1_g000031 Rmu_sc0010423.1_g000003
rosa_roxburghii Rroxscaffold_5G00335790 Rroxscaffold_5G00335850 Rroxscaffold_5G00335970 Rroxscaffold_5G00335990
rosa_rugosa Rorug03G0325100 Rorug03G0325300 Rorug03G0325500.1 Rorug03G0326800 Rorug03G0327000 Rorug04G0104400
rosa_samantha Rh4AG029900 Rh4AG030000 Rh4AG030100 Rh4AG030200 Rh4AG030600 Rh4AG172900 Rh4BG023200 Rh4BG023900 Rh4BG024000 Rh4BG024100 Rh4BG172200 Rh4DG023300 Rh4DG023800 Rh4DG023900 Rh4DG024000 Rh4DG151600
rosa_wichuraiana Rw1G002330 Rw1G002340 Rw4G002260 Rw4G002270 Rw4G002280 Rw4G002300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 480
AclWI GGATC 3 cut(s) 696, 883, 896
AcsI RAATTY 1 cut(s) 455
AfaI GTAC 1 cut(s) 235
AfiI CCNNNNNNNGG 1 cut(s) 72
AgsI TTSAA 3 cut(s) 634, 934, 984
AhlI ACTAGT 1 cut(s) 761
AjnI CCWGG 2 cut(s) 340, 465
AjuI GAANNNNNNNTTGG 2 cut(s) 266, 298
AleI CACNNNNGTG 1 cut(s) 368
AluBI AGCT 7 cut(s) 79, 388, 434, 475, 580, 829, 841
AluI AGCT 7 cut(s) 79, 388, 434, 475, 580, 829, 841
Alw21I GWGCWC 1 cut(s) 302
Alw26I GTCTC 1 cut(s) 869
Alw44I GTGCAC 1 cut(s) 298
AlwI GGATC 3 cut(s) 696, 883, 896
AoxI GGCC 3 cut(s) 7, 597, 789
ApaLI GTGCAC 1 cut(s) 298
ApeKI GCWGC 2 cut(s) 431, 472
ApoI RAATTY 1 cut(s) 455
AseI ATTAAT 1 cut(s) 941
AspLEI GCGC 3 cut(s) 16, 472, 561
AspS9I GGNCC 1 cut(s) 790
AsuHPI GGTGA 2 cut(s) 356, 464
BaeGI GKGCMC 1 cut(s) 302
BaeI ACNNNNGTAYC 2 cut(s) 225, 258
BamHI GGATCC 1 cut(s) 888
Bbv12I GWGCWC 1 cut(s) 302
BbvI GCAGC 2 cut(s) 443, 484
BccI CCATC 2 cut(s) 761, 971
BceAI ACGGC 1 cut(s) 526
BciT130I CCWGG 2 cut(s) 342, 467
BclI TGATCA 1 cut(s) 591
BcoDI GTCTC 1 cut(s) 869
BcuI ACTAGT 1 cut(s) 761
BfaI CTAG 4 cut(s) 5, 11, 428, 762
BfmI CTRYAG 2 cut(s) 678, 1010
BglII AGATCT 1 cut(s) 108
BisI GCNGC 2 cut(s) 432, 473
BlsI GCNGC 2 cut(s) 433, 474
Bme1390I CCNGG 2 cut(s) 342, 467
BmgT120I GGNCC 1 cut(s) 790
BmiI GGNNCC 3 cut(s) 464, 792, 890
BmrFI CCNGG 2 cut(s) 342, 467
BmsI GCATC 2 cut(s) 315, 425
BoxI GACNNNNGTC 1 cut(s) 134
BpuEI CTTGAG 4 cut(s) 179, 222, 521, 596
Bsa29I ATCGAT 1 cut(s) 975
BsaBI GATNNNNATC 1 cut(s) 783
BsaJI CCNNGG 1 cut(s) 30
BsaWI WCCGGW 1 cut(s) 697
Bsc4I CCNNNNNNNGG 1 cut(s) 72
Bse1I ACTGG 2 cut(s) 279, 628
Bse3DI GCAATG 2 cut(s) 66, 335
Bse8I GATNNNNATC 1 cut(s) 783
BseBI CCWGG 2 cut(s) 342, 467
BseCI ATCGAT 1 cut(s) 975
BseDI CCNNGG 1 cut(s) 30
BseGI GGATG 1 cut(s) 189
BseJI GATNNNNATC 1 cut(s) 783
BseLI CCNNNNNNNGG 1 cut(s) 72
BseMI GCAATG 2 cut(s) 66, 335
BseMII CTCAG 1 cut(s) 71
BseNI ACTGG 2 cut(s) 279, 628
BseRI GAGGAG 3 cut(s) 131, 524, 881
BseSI GKGCMC 1 cut(s) 302
BseXI GCAGC 2 cut(s) 443, 484
BsgI GTGCAG 2 cut(s) 248, 536
Bsh1236I CGCG 1 cut(s) 480
BshFI GGCC 3 cut(s) 9, 599, 791
BshVI ATCGAT 1 cut(s) 975
BsiHKAI GWGCWC 1 cut(s) 302
BsiSI CCGG 1 cut(s) 698
BslFI GGGAC 3 cut(s) 509, 650, 785
BslI CCNNNNNNNGG 1 cut(s) 72
BsmAI GTCTC 1 cut(s) 869
BsmFI GGGAC 3 cut(s) 509, 650, 785
BsmI GAATGC 1 cut(s) 623
BsnI GGCC 3 cut(s) 9, 599, 791
Bsp1286I GDGCHC 1 cut(s) 302
Bsp143I GATC 7 cut(s) 108, 591, 612, 688, 747, 784, 888
Bsp68I TCGCGA 1 cut(s) 480
BspANI GGCC 3 cut(s) 9, 599, 791
BspCNI CTCAG 1 cut(s) 70
BspDI ATCGAT 1 cut(s) 975
BspFNI CGCG 1 cut(s) 480
BspLI GGNNCC 3 cut(s) 464, 792, 890
BspPI GGATC 3 cut(s) 696, 883, 896
BsrDI GCAATG 2 cut(s) 66, 335
BsrI ACTGG 2 cut(s) 279, 628
BssECI CCNNGG 1 cut(s) 30
BssMI GATC 7 cut(s) 108, 591, 612, 688, 747, 784, 888
BssT1I CCWWGG 1 cut(s) 30
Bst2UI CCWGG 2 cut(s) 342, 467
Bst4CI ACNGT 4 cut(s) 43, 370, 727, 997
BstAPI GCANNNNNTGC 1 cut(s) 437
BstC8I GCNNGC 4 cut(s) 201, 290, 302, 335
BstDEI CTNAG 5 cut(s) 57, 80, 744, 842, 987
BstF5I GGATG 1 cut(s) 189
BstFNI CGCG 1 cut(s) 480
BstHHI GCGC 3 cut(s) 16, 472, 561
BstKTI GATC 7 cut(s) 111, 594, 615, 691, 750, 787, 891
BstMAI GTCTC 1 cut(s) 869
BstMBI GATC 7 cut(s) 108, 591, 612, 688, 747, 784, 888
BstMWI GCNNNNNNNGC 3 cut(s) 334, 437, 469
BstNI CCWGG 2 cut(s) 342, 467
BstNSI RCATGY 1 cut(s) 248
BstPAI GACNNNNGTC 1 cut(s) 134
BstSCI CCNGG 2 cut(s) 340, 465
BstSFI CTRYAG 2 cut(s) 678, 1010
BstSLI GKGCMC 1 cut(s) 302
BstUI CGCG 1 cut(s) 480
BstV1I GCAGC 2 cut(s) 443, 484
BstX2I RGATCY 2 cut(s) 108, 888
BstYI RGATCY 2 cut(s) 108, 888
Bsu15I ATCGAT 1 cut(s) 975
BsuRI GGCC 3 cut(s) 9, 599, 791
BsuTUI ATCGAT 1 cut(s) 975
BtsCI GGATG 1 cut(s) 189
BtsIMutI CAGTG 3 cut(s) 375, 419, 723
BtuMI TCGCGA 1 cut(s) 480
Cac8I GCNNGC 4 cut(s) 201, 290, 302, 335
CfoI GCGC 3 cut(s) 16, 472, 561
Cfr13I GGNCC 1 cut(s) 790
ClaI ATCGAT 1 cut(s) 975
Csp6I GTAC 1 cut(s) 234
CviAII CATG 6 cut(s) 94, 134, 140, 245, 402, 815
CviQI GTAC 1 cut(s) 234
DdeI CTNAG 5 cut(s) 57, 80, 744, 842, 987
DpnI GATC 7 cut(s) 110, 593, 614, 690, 749, 786, 890
DpnII GATC 7 cut(s) 108, 591, 612, 688, 747, 784, 888
Eco130I CCWWGG 1 cut(s) 30
Eco147I AGGCCT 1 cut(s) 9
EcoRI GAATTC 1 cut(s) 455
EcoRII CCWGG 2 cut(s) 340, 465
EcoT14I CCWWGG 1 cut(s) 30
EcoT22I ATGCAT 1 cut(s) 623
ErhI CCWWGG 1 cut(s) 30
FaeI CATG 6 cut(s) 97, 137, 143, 248, 405, 818
FalI AAGNNNNNCTT 2 cut(s) 95, 127
FaqI GGGAC 3 cut(s) 509, 650, 785
FatI CATG 6 cut(s) 93, 133, 139, 244, 401, 814
FbaI TGATCA 1 cut(s) 591
Fnu4HI GCNGC 2 cut(s) 432, 473
FokI GGATG 1 cut(s) 176
Fsp4HI GCNGC 2 cut(s) 432, 473
FspBI CTAG 4 cut(s) 5, 11, 428, 762
GlaI GCGC 3 cut(s) 15, 471, 560
GluI GCNGC 2 cut(s) 432, 473
HaeIII GGCC 3 cut(s) 9, 599, 791
HapII CCGG 1 cut(s) 698
HhaI GCGC 3 cut(s) 16, 472, 561
Hin1II CATG 6 cut(s) 97, 137, 143, 248, 405, 818
Hin6I GCGC 3 cut(s) 14, 470, 559
HinP1I GCGC 3 cut(s) 14, 470, 559
HinfI GANTC 8 cut(s) 130, 149, 164, 312, 482, 522, 835, 906
HpaII CCGG 1 cut(s) 698
HphI GGTGA 2 cut(s) 356, 464
Hpy166II GTNNAC 3 cut(s) 49, 250, 300
Hpy188I TCNGA 6 cut(s) 60, 100, 394, 487, 547, 591
Hpy188III TCNNGA 3 cut(s) 479, 575, 892
Hpy8I GTNNAC 3 cut(s) 49, 250, 300
HpyAV CCTTC 2 cut(s) 163, 879
HpyCH4III ACNGT 4 cut(s) 43, 370, 727, 997
HpyF10VI GCNNNNNNNGC 3 cut(s) 334, 437, 469
HpyF3I CTNAG 5 cut(s) 57, 80, 744, 842, 987
Hsp92II CATG 6 cut(s) 97, 137, 143, 248, 405, 818
HspAI GCGC 3 cut(s) 14, 470, 559
Ksp22I TGATCA 1 cut(s) 591
Kzo9I GATC 7 cut(s) 108, 591, 612, 688, 747, 784, 888
LmnI GCTCC 2 cut(s) 76, 468
Lsp1109I GCAGC 2 cut(s) 443, 484
LweI GCATC 2 cut(s) 315, 425
MaeI CTAG 4 cut(s) 5, 11, 428, 762
MaeIII GTNAC 2 cut(s) 344, 898
MalI GATC 7 cut(s) 110, 593, 614, 690, 749, 786, 890
MboI GATC 7 cut(s) 108, 591, 612, 688, 747, 784, 888
MboII GAAGA 1 cut(s) 118
MfeI CAATTG 1 cut(s) 504
MflI RGATCY 2 cut(s) 108, 888
MhlI GDGCHC 1 cut(s) 302
MluCI AATT 6 cut(s) 455, 504, 570, 670, 861, 918
MlyI GAGTC 2 cut(s) 124, 531
MnlI CCTC 8 cut(s) 109, 203, 348, 524, 545, 859, 862, 919
Mph1103I ATGCAT 1 cut(s) 623
MseI TTAA 4 cut(s) 582, 702, 822, 941
MslI CAYNNNNRTG 3 cut(s) 138, 144, 368
MspI CCGG 1 cut(s) 698
MspR9I CCNGG 2 cut(s) 342, 467
MunI CAATTG 1 cut(s) 504
Mva1269I GAATGC 1 cut(s) 623
MvaI CCWGG 2 cut(s) 342, 467
MvnI CGCG 1 cut(s) 480
MwoI GCNNNNNNNGC 3 cut(s) 334, 437, 469
NdeII GATC 7 cut(s) 108, 591, 612, 688, 747, 784, 888
NlaIII CATG 6 cut(s) 97, 137, 143, 248, 405, 818
NlaIV GGNNCC 3 cut(s) 464, 792, 890
NmuCI GTSAC 2 cut(s) 344, 898
NruI TCGCGA 1 cut(s) 480
NsiI ATGCAT 1 cut(s) 623
NspI RCATGY 1 cut(s) 248
OliI CACNNNNGTG 1 cut(s) 368
PceI AGGCCT 1 cut(s) 9
PctI GAATGC 1 cut(s) 623
PfeI GAWTC 6 cut(s) 149, 164, 312, 482, 835, 906
PkrI GCNGC 2 cut(s) 433, 474
PleI GAGTC 2 cut(s) 124, 530
PpsI GAGTC 2 cut(s) 124, 530
PshAI GACNNNNGTC 1 cut(s) 134
PshBI ATTAAT 1 cut(s) 941
Psp6I CCWGG 2 cut(s) 340, 465
PspGI CCWGG 2 cut(s) 340, 465
PspN4I GGNNCC 3 cut(s) 464, 792, 890
PspPI GGNCC 1 cut(s) 790
PsuI RGATCY 2 cut(s) 108, 888
RruI TCGCGA 1 cut(s) 480
RsaI GTAC 1 cut(s) 235
RsaNI GTAC 1 cut(s) 234
RseI CAYNNNNRTG 3 cut(s) 138, 144, 368
SaqAI TTAA 4 cut(s) 582, 702, 822, 941
SatI GCNGC 2 cut(s) 432, 473
Sau3AI GATC 7 cut(s) 108, 591, 612, 688, 747, 784, 888
Sau96I GGNCC 1 cut(s) 790
SchI GAGTC 2 cut(s) 124, 531
ScrFI CCNGG 2 cut(s) 342, 467
SduI GDGCHC 1 cut(s) 302
SfaNI GCATC 2 cut(s) 315, 425
SfcI CTRYAG 2 cut(s) 678, 1010
SmiMI CAYNNNNRTG 3 cut(s) 138, 144, 368
SmlI CTYRAG 4 cut(s) 194, 237, 536, 575
SmoI CTYRAG 4 cut(s) 194, 237, 536, 575
SpeI ACTAGT 1 cut(s) 761
Sse9I AATT 6 cut(s) 455, 504, 570, 670, 861, 918
SseBI AGGCCT 1 cut(s) 9
SspI AATATT 1 cut(s) 945
SspMI CTAG 4 cut(s) 5, 11, 428, 762
StuI AGGCCT 1 cut(s) 9
StyD4I CCNGG 2 cut(s) 340, 465
StyI CCWWGG 1 cut(s) 30
TaaI ACNGT 4 cut(s) 43, 370, 727, 997
TaqI TCGA 3 cut(s) 156, 615, 975
TasI AATT 6 cut(s) 455, 504, 570, 670, 861, 918
TfiI GAWTC 6 cut(s) 149, 164, 312, 482, 835, 906
Tru1I TTAA 4 cut(s) 582, 702, 822, 941
Tru9I TTAA 4 cut(s) 582, 702, 822, 941
TscAI CASTG 3 cut(s) 375, 426, 730
TseFI GTSAC 2 cut(s) 344, 898
TseI GCWGC 2 cut(s) 431, 472
Tsp45I GTSAC 2 cut(s) 344, 898
TspDTI ATGAA 2 cut(s) 162, 390
TspRI CASTG 3 cut(s) 375, 426, 730
VneI GTGCAC 1 cut(s) 298
VspI ATTAAT 1 cut(s) 941
XapI RAATTY 1 cut(s) 455
XceI RCATGY 1 cut(s) 248
XspI CTAG 4 cut(s) 5, 11, 428, 762
Zsp2I ATGCAT 1 cut(s) 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.