FvH4_4g02460

Sieve element occlusion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000511
Physical Location & Seq
Forward (+)
2266060 .. 2269689
3630 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g02460.t3

Sequence Viewer

Length: 2043 bp
ATGCTCGGCCTAGCAAACAATGTGACTACCAAGGTGGCCTCAGTAGTCCACTCTACTCAGCAGACCATTGCTGGGGAGCTTAGCCTGTTTGCTATGTCTGATAAGAAAATCTTGGATGAGATTTACACAACTCATGTCCATGCTGATGAATCATTTGATGATGATTCTCTCTTCGTCATTGTTGAGAACATTCTTAAGCGTGCAACTCAAACGGTTGACAAAATTGTACAGGGTACCCAAGTGCATGTGGAAGACATAGAGGAGAAGATCCCCAAACCCAACTTCAGTGTACCACTGTGCACTCTTAAGGGAATCTCATGCGAGATGCAATGCAAGCCTCCTGGTGAGGAAATCGCCCACAAGACAACACTGGCGATACTCAACAAGCTGTCACATTATTCATGGGAAGCGAAGGCATCACTGACCCTAGCAGCTTTTGCCATGGAATTTGGTGAATTCTGGCTCCTCGCAGACCTTCGCGAATCAAACCACCTTGCCAAATCAATTGCAATTCTCAAACGAGTCCCTGTTCTCCTCAAGCCTTCTCAGCTGCAAAAGCGCAGGCAAGCAATACTTGAGCTTAACAATCTGATCAAGGCAACATTGCAAGTGATTGAATGCATCGACCAGTTCGGCAAGCTTTCCGTTTATCCCCCAAAAGATGTCCCTGCTTTGTCGATAGCATTGGAACACACCCCGGTGGATATCTATTGGGCTATCATGACTGTTGTGGCTTGTGCCACTAAGGTCACTTTACTCACTAGTGATGAGGATAAGGAATTTGATCTATCTCCCTATTCTCAAAAGATTCACTATGTGCTTAACAAGCTCAAGATTCAGCTGATAGTTTGCCACAAACAAATTGAGGAGGCAAAGAATCACAGATATATCTTAAATCTGCTTCGAAGACCTACTGAAATTATGGAAGTTTTTAAGGGTTTGATATTTACAAAGGATAATGTCCAGCCACTGATTGATGGTTCCACTAAACAAACGGTTAACATCGACGTCCTAAGGAGGAAGAATGTGCTACTGTTCATTTCCGGCCTAGACATTACGGATGATGACATTTCGATTCTGAAACCGATCCATGAGCTCACAAAGAAAGAGAATCAGTATAAGATTGTCTGGGTGCCAATTGTGGAGAAATGGACTGATGAACTTAGAAAGAAGTTTGAAATCCTGAAGAACAAGATGCCTTGGTTTACAGTGCAGCAACCTGGACCTATAGCAGGTATCAAGGTCATTAAGGAGGAGTGGAACTTCAAGGGTAAGCCTACATTGGTGGTGATGAACCCACAAGGAAAGGTCGAACATTCTAACGCTCTCCACATGATTCGGGTGTGGGGAGTCAAGGCCTTCCCTTTCACAGAGAAAATAGAAAAAGAGCTTTCACATTCTCATGGAGAAATTCACTCTGTTGTTGAAGGAGTTCACCCATCTGTGCCATCTTGGCTCAAAGAAAATAGGTACATCTTCTTCTATGGCGGTAAAGACAACGAATGGATTCAGCAATTCACAAAGAAAGCAACTGCCCTTGCCAATGATCCAATCTTGAAGGAAGCAAAGATTTACATAGAGTTGGTATGCGTTGGAAAAAACAGCAAAGGAGAAGATGACAATGGCATTCTAGGCCGCTTCTGGACCAATATAGAAAGCTTGTTCTTGACCAAAGTGGACAAGCAGGTCGACTCAGTAAGTCAAGAAATCCAGAAGCTTCTCTCTTACAAGAACGAAAGTGGATGGGCGGTGCTTACCAGAGGATCGACTGTGTTGGTCACAGGTCATGGTGTCTCGGTATTGAAGGTGGTAGAGGACTTTGAGAAATGGAAGGAGCATGTCAAGGAGAAAGGGTTTGAGTTTTGTTTCAAGGCCTATTATGGGAGGGTAGTTCAAGCTAGTCGACCCTGCTGCCGTCTTGACATCCCGGGGTCTACCGGAAAGGTGCCGGAGTCAATGAAGTGCCCGGATTGCCAGCGTAATATGGAGACTTATATCAGTTACAAGTGCTGCCACATTGATGGTCCTACCGCACATCACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SEO_C SEO_N

Protein Analysis

681

Amino Acids

77.28

Weight (kDa)

8.04

Isoelectric Point (pI)

36.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SEO_N PF14576 33 - 300 5.2e-82 Sieve element occlusion N-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000511)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g02370 FvH4_4g02370 FvH4_4g02370 FvH4_4g02430 FvH4_4g02430 FvH4_4g02460 FvH4_4g02460 FvH4_4g02460 FvH4_4g02480 FvH4_4g02480 FvH4_4g02480 FvH4_4g02480 FvH4_6g05920
malus_domestica MD00G1111000.v1.1 MD08G1225800.v1.1 MD13G1205700.v1.1 MD13G1205800.v1.1 MD13G1205900.v1.1 MD13G1206000.v1.1 MD16G1207500.v1.1 MD16G1207600.v1.1
prunus_persica Prupe.1G028400_v2.0.a1 Prupe.1G028500_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028900_v2.0.a1 Prupe.1G029000_v2.0.a1 Prupe.1G029100_v2.0.a1
pyrus_communis pycom08g19680 pycom08g19730 pycom16g17460 pycom16g17500
rosa_chinensis RchiOBHm_Chr4g0389751 RchiOBHm_Chr4g0389771 RchiOBHm_Chr4g0389791 RchiOBHm_Chr4g0389861 RchiOBHm_Chr4g0389881 RchiOBHm_Chr4g0389901 RchiOBHm_Chr4g0412411
rosa_laevigata RLG00000008288 RLG00000009901 RLG00000009945 RLG00000009954 RLG00000009956 RLG00000009957 RLG00000009958 RLG00000019246
rosa_multiflora Rmu_co8275125.1_g000001 Rmu_sc0002083.1_g000003 Rmu_sc0002083.1_g000025 Rmu_sc0002604.1_g000016 Rmu_sc0004336.1_g000031 Rmu_sc0010423.1_g000003
rosa_roxburghii Rroxscaffold_5G00335790 Rroxscaffold_5G00335850 Rroxscaffold_5G00335970 Rroxscaffold_5G00335990
rosa_rugosa Rorug03G0325100 Rorug03G0325300 Rorug03G0325500.1 Rorug03G0326800 Rorug03G0327000 Rorug04G0104400
rosa_samantha Rh4AG029900 Rh4AG030000 Rh4AG030100 Rh4AG030200 Rh4AG030600 Rh4AG172900 Rh4BG023200 Rh4BG023900 Rh4BG024000 Rh4BG024100 Rh4BG172200 Rh4DG023300 Rh4DG023800 Rh4DG023900 Rh4DG024000 Rh4DG151600
rosa_wichuraiana Rw1G002330 Rw1G002340 Rw4G002260 Rw4G002270 Rw4G002280 Rw4G002300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1685
AatII GACGTC 1 cut(s) 1011
Acc36I ACCTGC 2 cut(s) 1223, 1675
Acc65I GGTACC 1 cut(s) 233
AccB1I GGYRCC 3 cut(s) 233, 1132, 1945
AccI GTMKAC 3 cut(s) 1689, 1903, 1934
AccII CGCG 1 cut(s) 480
AciI CCGC 4 cut(s) 1488, 1636, 1748, 2031
AclWI GGATC 4 cut(s) 262, 1081, 1541, 1771
AcsI RAATTY 4 cut(s) 446, 455, 779, 1410
AcuI CTGAAG 2 cut(s) 268, 1205
AcyI GRCGYC 1 cut(s) 1008
AdeI CACNNNGTG 1 cut(s) 817
AfaI GTAC 4 cut(s) 228, 235, 291, 1472
AfiI CCNNNNNNNGG 3 cut(s) 72, 1232, 1881
AflII CTTAAG 2 cut(s) 194, 305
AgsI TTSAA 8 cut(s) 617, 1178, 1267, 1427, 1558, 1804, 1870, 1895
AhlI ACTAGT 1 cut(s) 761
AjnI CCWGG 2 cut(s) 340, 1219
AjuI GAANNNNNNNTTGG 2 cut(s) 266, 298
AleI CACNNNNGTG 1 cut(s) 698
Alw21I GWGCWC 2 cut(s) 302, 1098
Alw26I GTCTC 2 cut(s) 1798, 1982
Alw44I GTGCAC 1 cut(s) 298
AlwI GGATC 4 cut(s) 262, 1081, 1541, 1771
AlwNI CAGNNNCTG 1 cut(s) 970
Ama87I CYCGRG 1 cut(s) 1927
AoxI GGCC 6 cut(s) 7, 36, 1045, 1356, 1633, 1872
ApaLI GTGCAC 1 cut(s) 298
ApeKI GCWGC 5 cut(s) 431, 550, 1213, 1911, 2010
ApoI RAATTY 4 cut(s) 446, 455, 779, 1410
Asp700I GAANNNNTTC 2 cut(s) 1431, 1506
Asp718I GGTACC 1 cut(s) 233
AspLEI GCGC 1 cut(s) 561
AspS9I GGNCC 3 cut(s) 1223, 1644, 2024
AsuC2I CCSGG 4 cut(s) 698, 1928, 1929, 1967
AsuHPI GGTGA 4 cut(s) 356, 464, 1300, 1427
AsuII TTCGAA 1 cut(s) 904
AvaI CYCGRG 1 cut(s) 1927
AvaII GGWCC 3 cut(s) 1223, 1644, 2024
AxyI CCTNAGG 1 cut(s) 1013
BaeGI GKGCMC 2 cut(s) 302, 1967
BaeI ACNNNNGTAYC 2 cut(s) 225, 258
BanI GGYRCC 3 cut(s) 233, 1132, 1945
BanII GRGCYC 1 cut(s) 1098
BarI GAAGNNNNNNTAC 2 cut(s) 1463, 1495
BbsI GAAGAC 2 cut(s) 258, 913
Bbv12I GWGCWC 2 cut(s) 302, 1098
BbvI GCAGC 5 cut(s) 443, 537, 1225, 1898, 1997
BccI CCATC 5 cut(s) 971, 1447, 1456, 1737, 2015
BceAI ACGGC 1 cut(s) 1899
BcgI CGANNNNNNTGC 2 cut(s) 1893, 1927
BciT130I CCWGG 2 cut(s) 342, 1221
BclI TGATCA 1 cut(s) 591
BcnI CCSGG 4 cut(s) 698, 1928, 1929, 1967
BcoDI GTCTC 2 cut(s) 1798, 1982
BcuI ACTAGT 1 cut(s) 761
BfaI CTAG 6 cut(s) 11, 428, 762, 1049, 1631, 1899
BfmI CTRYAG 1 cut(s) 1227
BfrI CTTAAG 2 cut(s) 194, 305
BfuAI ACCTGC 2 cut(s) 1223, 1675
BglI GCCNNNNNGGC 1 cut(s) 1453
BisI GCNGC 6 cut(s) 432, 551, 1214, 1636, 1912, 2011
BlpI GCTNAGC 1 cut(s) 80
BlsI GCNGC 6 cut(s) 433, 552, 1215, 1637, 1913, 2012
Bme1390I CCNGG 6 cut(s) 342, 698, 1221, 1928, 1929, 1967
Bme18I GGWCC 3 cut(s) 1223, 1644, 2024
BmeT110I CYCGRG 1 cut(s) 1927
BmgT120I GGNCC 3 cut(s) 1223, 1644, 2024
BmiI GGNNCC 5 cut(s) 235, 464, 982, 1134, 1947
BmrFI CCNGG 6 cut(s) 342, 698, 1221, 1928, 1929, 1967
BmsI GCATC 4 cut(s) 315, 425, 630, 1185
BpiI GAAGAC 2 cut(s) 258, 913
Bpu1102I GCTNAGC 1 cut(s) 80
Bpu14I TTCGAA 1 cut(s) 904
BpuEI CTTGAG 3 cut(s) 521, 596, 815
BpuMI CCSGG 4 cut(s) 698, 1928, 1929, 1967
BsaHI GRCGYC 1 cut(s) 1008
BsaJI CCNNGG 6 cut(s) 30, 441, 696, 1199, 1927, 1928
BsaWI WCCGGW 1 cut(s) 1937
BsaXI ACNNNNNCTCC 2 cut(s) 1838, 1868
Bsc4I CCNNNNNNNGG 3 cut(s) 72, 1232, 1881
Bse1I ACTGG 2 cut(s) 375, 628
Bse21I CCTNAGG 1 cut(s) 1013
Bse3DI GCAATG 3 cut(s) 66, 335, 602
BseBI CCWGG 2 cut(s) 342, 1221
BseDI CCNNGG 6 cut(s) 30, 441, 696, 1199, 1927, 1928
BseGI GGATG 4 cut(s) 121, 1066, 1748, 1923
BseLI CCNNNNNNNGG 3 cut(s) 72, 1232, 1881
BseMI GCAATG 3 cut(s) 66, 335, 602
BseMII CTCAG 4 cut(s) 54, 71, 560, 1707
BseNI ACTGG 2 cut(s) 375, 628
BseRI GAGGAG 5 cut(s) 275, 455, 524, 881, 1268
BseSI GKGCMC 2 cut(s) 302, 1967
BseXI GCAGC 5 cut(s) 443, 537, 1225, 1898, 1997
BseYI CCCAGC 1 cut(s) 71
BsgI GTGCAG 1 cut(s) 1232
Bsh1236I CGCG 1 cut(s) 480
BshFI GGCC 6 cut(s) 9, 38, 1047, 1358, 1635, 1874
BshNI GGYRCC 3 cut(s) 233, 1132, 1945
BsiHKAI GWGCWC 2 cut(s) 302, 1098
BsiHKCI CYCGRG 1 cut(s) 1927
BsiSI CCGG 6 cut(s) 698, 1044, 1928, 1938, 1949, 1967
BslFI GGGAC 2 cut(s) 509, 650
BslI CCNNNNNNNGG 3 cut(s) 72, 1232, 1881
BsmAI GTCTC 2 cut(s) 1798, 1982
BsmFI GGGAC 2 cut(s) 509, 650
BsmI GAATGC 2 cut(s) 623, 1626
BsnI GGCC 6 cut(s) 9, 38, 1047, 1358, 1635, 1874
BsoBI CYCGRG 1 cut(s) 1927
Bsp119I TTCGAA 1 cut(s) 904
Bsp1286I GDGCHC 3 cut(s) 302, 1098, 1967
Bsp1407I TGTACA 1 cut(s) 226
Bsp143I GATC 6 cut(s) 267, 591, 784, 1086, 1546, 1763
Bsp1720I GCTNAGC 1 cut(s) 80
Bsp19I CCATGG 1 cut(s) 441
Bsp68I TCGCGA 1 cut(s) 480
BspACI CCGC 4 cut(s) 1488, 1636, 1748, 2031
BspANI GGCC 6 cut(s) 9, 38, 1047, 1358, 1635, 1874
BspCNI CTCAG 4 cut(s) 53, 70, 559, 1706
BspFNI CGCG 1 cut(s) 480
BspHI TCATGA 1 cut(s) 720
BspLI GGNNCC 5 cut(s) 235, 464, 982, 1134, 1947
BspMI ACCTGC 2 cut(s) 1223, 1675
BspPI GGATC 4 cut(s) 262, 1081, 1541, 1771
BspT104I TTCGAA 1 cut(s) 904
BspT107I GGYRCC 3 cut(s) 233, 1132, 1945
BspTI CTTAAG 2 cut(s) 194, 305
BsrDI GCAATG 3 cut(s) 66, 335, 602
BsrGI TGTACA 1 cut(s) 226
BsrI ACTGG 2 cut(s) 375, 628
BssECI CCNNGG 6 cut(s) 30, 441, 696, 1199, 1927, 1928
BssMI GATC 6 cut(s) 267, 591, 784, 1086, 1546, 1763
BssNI GRCGYC 1 cut(s) 1008
BssT1I CCWWGG 3 cut(s) 30, 441, 1199
Bst2UI CCWGG 2 cut(s) 342, 1221
Bst4CI ACNGT 7 cut(s) 214, 297, 727, 997, 1035, 1210, 1771
Bst6I CTCTTC 1 cut(s) 176
BstACI GRCGYC 1 cut(s) 1008
BstAFI CTTAAG 2 cut(s) 194, 305
BstAPI GCANNNNNTGC 1 cut(s) 437
BstAUI TGTACA 1 cut(s) 226
BstBI TTCGAA 1 cut(s) 904
BstC8I GCNNGC 6 cut(s) 201, 335, 563, 567, 638, 1976
BstDEI CTNAG 8 cut(s) 40, 57, 80, 546, 744, 1013, 1163, 1693
BstDSI CCRYGG 1 cut(s) 441
BstENI CCTNNNNNAGG 1 cut(s) 1230
BstF5I GGATG 4 cut(s) 121, 1066, 1748, 1923
BstFNI CGCG 1 cut(s) 480
BstHHI GCGC 1 cut(s) 561
BstKTI GATC 6 cut(s) 270, 594, 787, 1089, 1549, 1766
BstMAI GTCTC 2 cut(s) 1798, 1982
BstMBI GATC 6 cut(s) 267, 591, 784, 1086, 1546, 1763
BstMWI GCNNNNNNNGC 8 cut(s) 334, 437, 547, 556, 826, 1453, 1632, 1971
BstNI CCWGG 2 cut(s) 342, 1221
BstNSI RCATGY 2 cut(s) 248, 1841
BstSCI CCNGG 6 cut(s) 340, 696, 1219, 1926, 1927, 1965
BstSFI CTRYAG 1 cut(s) 1227
BstSLI GKGCMC 2 cut(s) 302, 1967
BstUI CGCG 1 cut(s) 480
BstV1I GCAGC 5 cut(s) 443, 537, 1225, 1898, 1997
BstV2I GAAGAC 2 cut(s) 258, 913
BstX2I RGATCY 1 cut(s) 267
BstXI CCANNNNNNTGG 1 cut(s) 2021
BstYI RGATCY 1 cut(s) 267
Bsu36I CCTNAGG 1 cut(s) 1013
BsuRI GGCC 6 cut(s) 9, 38, 1047, 1358, 1635, 1874
BtgI CCRYGG 1 cut(s) 441
BtsCI GGATG 4 cut(s) 121, 1066, 1748, 1923
BtsIMutI CAGTG 6 cut(s) 292, 293, 368, 419, 968, 1215
BtuMI TCGCGA 1 cut(s) 480
BveI ACCTGC 2 cut(s) 1223, 1675
Cac8I GCNNGC 6 cut(s) 201, 335, 563, 567, 638, 1976
CaiI CAGNNNCTG 1 cut(s) 970
CciI TCATGA 1 cut(s) 720
CfoI GCGC 1 cut(s) 561
Cfr13I GGNCC 3 cut(s) 1223, 1644, 2024
Cfr9I CCCGGG 1 cut(s) 1927
Csp6I GTAC 4 cut(s) 227, 234, 290, 1471
CviQI GTAC 4 cut(s) 227, 234, 290, 1471
DdeI CTNAG 8 cut(s) 40, 57, 80, 546, 744, 1013, 1163, 1693
DpnI GATC 6 cut(s) 269, 593, 786, 1088, 1548, 1765
DpnII GATC 6 cut(s) 267, 591, 784, 1086, 1546, 1763
DraIII CACNNNGTG 1 cut(s) 817
DrdI GACNNNNNNGTC 1 cut(s) 1685
DseDI GACNNNNNNGTC 1 cut(s) 1685
Eam1104I CTCTTC 1 cut(s) 176
EarI CTCTTC 1 cut(s) 176
Ecl136II GAGCTC 1 cut(s) 1096
Eco130I CCWWGG 3 cut(s) 30, 441, 1199
Eco147I AGGCCT 2 cut(s) 1358, 1874
Eco24I GRGCYC 1 cut(s) 1098
Eco32I GATATC 1 cut(s) 706
Eco47I GGWCC 3 cut(s) 1223, 1644, 2024
Eco53kI GAGCTC 1 cut(s) 1096
Eco57I CTGAAG 2 cut(s) 268, 1205
Eco81I CCTNAGG 1 cut(s) 1013
Eco88I CYCGRG 1 cut(s) 1927
EcoICRI GAGCTC 1 cut(s) 1096
EcoNI CCTNNNNNAGG 1 cut(s) 1230
EcoRI GAATTC 1 cut(s) 455
EcoRII CCWGG 2 cut(s) 340, 1219
EcoRV GATATC 1 cut(s) 706
EcoT14I CCWWGG 3 cut(s) 30, 441, 1199
EcoT22I ATGCAT 1 cut(s) 623
EcoT38I GRGCYC 1 cut(s) 1098
ErhI CCWWGG 3 cut(s) 30, 441, 1199
FalI AAGNNNNNCTT 4 cut(s) 95, 127, 558, 590
FaqI GGGAC 2 cut(s) 509, 650
FbaI TGATCA 1 cut(s) 591
FblI GTMKAC 3 cut(s) 1689, 1903, 1934
Fnu4HI GCNGC 6 cut(s) 432, 551, 1214, 1636, 1912, 2011
FokI GGATG 4 cut(s) 128, 1073, 1755, 1910
FriOI GRGCYC 1 cut(s) 1098
Fsp4HI GCNGC 6 cut(s) 432, 551, 1214, 1636, 1912, 2011
FspBI CTAG 6 cut(s) 11, 428, 762, 1049, 1631, 1899
GlaI GCGC 1 cut(s) 560
GluI GCNGC 6 cut(s) 432, 551, 1214, 1636, 1912, 2011
GsaI CCCAGC 1 cut(s) 75
HaeIII GGCC 6 cut(s) 9, 38, 1047, 1358, 1635, 1874
HapII CCGG 6 cut(s) 698, 1044, 1928, 1938, 1949, 1967
HhaI GCGC 1 cut(s) 561
Hin1I GRCGYC 1 cut(s) 1008
Hin6I GCGC 1 cut(s) 559
HinP1I GCGC 1 cut(s) 559
HincII GTYRAC 4 cut(s) 217, 1000, 1690, 1904
HindII GTYRAC 4 cut(s) 217, 1000, 1690, 1904
HindIII AAGCTT 3 cut(s) 638, 1657, 1715
HpaI GTTAAC 1 cut(s) 1000
HpaII CCGG 6 cut(s) 698, 1044, 1928, 1938, 1949, 1967
HphI GGTGA 4 cut(s) 356, 464, 1300, 1427
Hpy188I TCNGA 3 cut(s) 100, 591, 1080
Hpy99I CGWCG 1 cut(s) 1010
HpyAV CCTTC 8 cut(s) 406, 485, 552, 1369, 1421, 1552, 1798, 1825
HpyCH4III ACNGT 7 cut(s) 214, 297, 727, 997, 1035, 1210, 1771
HpyCH4IV ACGT 1 cut(s) 1008
HpyF10VI GCNNNNNNNGC 8 cut(s) 334, 437, 547, 556, 826, 1453, 1632, 1971
HpyF3I CTNAG 8 cut(s) 40, 57, 80, 546, 744, 1013, 1163, 1693
HpySE526I ACGT 1 cut(s) 1008
Hsp92I GRCGYC 1 cut(s) 1008
HspAI GCGC 1 cut(s) 559
KpnI GGTACC 1 cut(s) 237
Ksp22I TGATCA 1 cut(s) 591
KspAI GTTAAC 1 cut(s) 1000
Kzo9I GATC 6 cut(s) 267, 591, 784, 1086, 1546, 1763
LmnI GCTCC 3 cut(s) 76, 468, 1834
Lsp1109I GCAGC 5 cut(s) 443, 537, 1225, 1898, 1997
LweI GCATC 4 cut(s) 315, 425, 630, 1185
MaeI CTAG 6 cut(s) 11, 428, 762, 1049, 1631, 1899
MaeII ACGT 1 cut(s) 1008
MaeIII GTNAC 5 cut(s) 22, 390, 748, 1777, 2000
MalI GATC 6 cut(s) 269, 593, 786, 1088, 1548, 1765
MboI GATC 6 cut(s) 267, 591, 784, 1086, 1546, 1763
MboII GAAGA 9 cut(s) 163, 263, 277, 918, 1033, 1198, 1468, 1471, 1625
MfeI CAATTG 2 cut(s) 504, 1137
MflI RGATCY 1 cut(s) 267
MhlI GDGCHC 3 cut(s) 302, 1098, 1967
MlyI GAGTC 4 cut(s) 531, 1359, 1685, 1961
MmeI TCCRAC 1 cut(s) 1573
Mph1103I ATGCAT 1 cut(s) 623
MroXI GAANNNNTTC 2 cut(s) 1431, 1506
MseI TTAA 8 cut(s) 195, 306, 582, 822, 893, 933, 999, 1248
MslI CAYNNNNRTG 5 cut(s) 138, 144, 698, 1401, 2019
MspA1I CMGCKG 2 cut(s) 550, 841
MspCI CTTAAG 2 cut(s) 194, 305
MspI CCGG 6 cut(s) 698, 1044, 1928, 1938, 1949, 1967
MspR9I CCNGG 6 cut(s) 342, 698, 1221, 1928, 1929, 1967
MunI CAATTG 2 cut(s) 504, 1137
Mva1269I GAATGC 2 cut(s) 623, 1626
MvaI CCWGG 2 cut(s) 342, 1221
MvnI CGCG 1 cut(s) 480
MwoI GCNNNNNNNGC 8 cut(s) 334, 437, 547, 556, 826, 1453, 1632, 1971
NciI CCSGG 4 cut(s) 698, 1928, 1929, 1967
NcoI CCATGG 1 cut(s) 441
NdeII GATC 6 cut(s) 267, 591, 784, 1086, 1546, 1763
NlaIV GGNNCC 5 cut(s) 235, 464, 982, 1134, 1947
NmuCI GTSAC 4 cut(s) 22, 390, 748, 1777
NruI TCGCGA 1 cut(s) 480
NsiI ATGCAT 1 cut(s) 623
NspI RCATGY 2 cut(s) 248, 1841
NspV TTCGAA 1 cut(s) 904
OliI CACNNNNGTG 1 cut(s) 698
PagI TCATGA 1 cut(s) 720
PceI AGGCCT 2 cut(s) 1358, 1874
PctI GAATGC 2 cut(s) 623, 1626
PdmI GAANNNNTTC 2 cut(s) 1431, 1506
PkrI GCNGC 6 cut(s) 433, 552, 1215, 1637, 1913, 2012
PleI GAGTC 4 cut(s) 530, 1358, 1685, 1960
PpsI GAGTC 4 cut(s) 530, 1358, 1685, 1960
Psp124BI GAGCTC 1 cut(s) 1098
Psp6I CCWGG 2 cut(s) 340, 1219
PspFI CCCAGC 1 cut(s) 71
PspGI CCWGG 2 cut(s) 340, 1219
PspN4I GGNNCC 5 cut(s) 235, 464, 982, 1134, 1947
PspPI GGNCC 3 cut(s) 1223, 1644, 2024
PstNI CAGNNNCTG 1 cut(s) 970
PsuI RGATCY 1 cut(s) 267
PvuII CAGCTG 2 cut(s) 550, 841
RruI TCGCGA 1 cut(s) 480
RsaI GTAC 4 cut(s) 228, 235, 291, 1472
RsaNI GTAC 4 cut(s) 227, 234, 290, 1471
RseI CAYNNNNRTG 5 cut(s) 138, 144, 698, 1401, 2019
SacI GAGCTC 1 cut(s) 1098
SalI GTCGAC 2 cut(s) 1688, 1902
SaqAI TTAA 8 cut(s) 195, 306, 582, 822, 893, 933, 999, 1248
SatI GCNGC 6 cut(s) 432, 551, 1214, 1636, 1912, 2011
Sau3AI GATC 6 cut(s) 267, 591, 784, 1086, 1546, 1763
Sau96I GGNCC 3 cut(s) 1223, 1644, 2024
SchI GAGTC 4 cut(s) 531, 1359, 1685, 1961
ScrFI CCNGG 6 cut(s) 342, 698, 1221, 1928, 1929, 1967
SduI GDGCHC 3 cut(s) 302, 1098, 1967
SfaNI GCATC 4 cut(s) 315, 425, 630, 1185
SfcI CTRYAG 1 cut(s) 1227
SfuI TTCGAA 1 cut(s) 904
SinI GGWCC 3 cut(s) 1223, 1644, 2024
SmaI CCCGGG 1 cut(s) 1929
SmiMI CAYNNNNRTG 5 cut(s) 138, 144, 698, 1401, 2019
SmlI CTYRAG 5 cut(s) 194, 305, 536, 575, 830
SmoI CTYRAG 5 cut(s) 194, 305, 536, 575, 830
SpeI ACTAGT 1 cut(s) 761
SseBI AGGCCT 2 cut(s) 1358, 1874
SsiI CCGC 4 cut(s) 1488, 1636, 1748, 2031
SspMI CTAG 6 cut(s) 11, 428, 762, 1049, 1631, 1899
SstI GAGCTC 1 cut(s) 1098
StuI AGGCCT 2 cut(s) 1358, 1874
StyD4I CCNGG 6 cut(s) 340, 696, 1219, 1926, 1927, 1965
StyI CCWWGG 3 cut(s) 30, 441, 1199
TaaI ACNGT 7 cut(s) 214, 297, 727, 997, 1035, 1210, 1771
TaiI ACGT 1 cut(s) 1011
TaqI TCGA 9 cut(s) 624, 677, 904, 1005, 1073, 1311, 1689, 1766, 1903
TatI WGTACW 1 cut(s) 226
TauI GCSGC 1 cut(s) 1638
Tru1I TTAA 8 cut(s) 195, 306, 582, 822, 893, 933, 999, 1248
Tru9I TTAA 8 cut(s) 195, 306, 582, 822, 893, 933, 999, 1248
TscAI CASTG 6 cut(s) 292, 300, 375, 426, 975, 1215
TseFI GTSAC 4 cut(s) 22, 390, 748, 1777
TseI GCWGC 5 cut(s) 431, 550, 1213, 1911, 2010
Tsp45I GTSAC 4 cut(s) 22, 390, 748, 1777
TspDTI ATGAA 6 cut(s) 162, 390, 1027, 1173, 1307, 1973
TspGWI ACGGA 2 cut(s) 634, 1073
TspMI CCCGGG 1 cut(s) 1927
TspRI CASTG 6 cut(s) 292, 300, 375, 426, 975, 1215
Vha464I CTTAAG 2 cut(s) 194, 305
VneI GTGCAC 1 cut(s) 298
VpaK11BI GGWCC 3 cut(s) 1223, 1644, 2024
XagI CCTNNNNNAGG 1 cut(s) 1230
XapI RAATTY 4 cut(s) 446, 455, 779, 1410
XceI RCATGY 2 cut(s) 248, 1841
XmaI CCCGGG 1 cut(s) 1927
XmiI GTMKAC 3 cut(s) 1689, 1903, 1934
XmnI GAANNNNTTC 2 cut(s) 1431, 1506
XspI CTAG 6 cut(s) 11, 428, 762, 1049, 1631, 1899
ZraI GACGTC 1 cut(s) 1009
Zsp2I ATGCAT 1 cut(s) 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.