Rh4DG024000

Sieve element occlusion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000511
Physical Location & Seq
Forward (+)
4062373 .. 4429271
366899 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG024000.1

Sequence Viewer

Length: 2052 bp
ATGCTAGGCCTAGCACACAATGTGGCTACCAAGGTAGCCTCGGTAGTCCACACTACTCAGAAAACCATTGCTGGGGAGCTTAGCCTGTTTGCCATGCCTGACAAGAAGATATTGGAGGAGATTTACACAACTCATGTCCACGCTGATGAATCATTCGATGATGATTCTCTCTTTGTCATTGTTGAGAACATCCTCAAGCGTGCAACCCAAAATGTTGATAAAATAGTGCAGGGTACTCAAGTGCATGTGGACAACATAGAGGAGAAAACTCCCAAAGCCAGTTTCAGCGTACCATTGTGCACGCTTAAGAGAATTTCCTGCGAGATGCAATGCAAGCCTCCTGGTGACGAAATCGCCCACAACACAACAGTGGCAATACTCAACAAGCTCTCAAAGTATTCATGGGAAGCAAAGGCATCACTGACCCTAGCAGCTTTTGCTATGGAATATGGTGAATTCTGGCTCCTGGCGCAGCTTCGCGAATCAGACCACCTTGCCAAATCAATTGCAATCCTCAAACGAGTCCCTGTTCTCCTCAAGCCGTCAGAGCTGCACAAGCGCAGGCAATCAATTCTTGAGCTTAACAATCTGATCAAGGCCACACTGCAAGTGATCGAATGCATCGACCAGTTCAATAAGTATTCCACTTATGACCCAAAAGATGTCCCCGATTTGTCGATAGCCTTGGATCATATTCCGGTTGATGTCTATTGGGTTATCATCACTGTTGTTGCTTGTGCCACTAAGATGACTATACTCACTAGTGATGAGGACAAGGAGTATGATCTAGCCCCCTATTCTCAAAAAATCCACTATGTCCTAAACAAGCTAACGATTCAGCTAAGAGGTTGCAGGAAACAAATTGAGGAGGCAGAGACTTACAGAAGGATCACGAAATTGTTCCGAACCCCTACTGAAATTATGGAGGTTTTCAAGGGACTGATATTTACAAAGGATAATGTCCAGCCACTCATAGATGGTTCAACTAAGCAAACGGTTAAGATCGACATCCTAAGGAGAAAGAATGTGTTATTGTTCATTTCCACCCTAGACGTCTCTGACGATGACATTTCGATTCTGAAACCGATCCACGAGTTCACAAAGAGAGACAATCAGTACAAGATTGTCTGGATTCCAATTGTGGAGCAATGGACTGATGACCTGAGAAAAAAATTTGATATCCTGAAGAACAAAATGCCTTGGTTTACGCTGCAGTACTCTGGACCTATTGCAGGCATCAAGTTCATCAAGGAGGAGTGGAATTTCAAGGGTAAGCCTACGGTAGTGGTGATGAACCCACAAGGAAAGGTCGAACACTCCAACGCTCTGCACATGATTCGGGTATGGGGAGCCAAGGCCTTCCCTTTCACAAAGACAACAGAAGAAGAACTGTCACATTCTCATGCAGGAAAATGGGTTGGCTCCGTTGTTGAAGGAACTCACCCATCTGTGCACACTTGGATCAAGGAGGAGAAGTATATTTTCTTCTACGGAGGCAAGGACAATGAATGGATTCAGCAATTCACAAAGAAAGCAACTGCCATTGCAAATGATCCTTTTATTAAGGAACTTAAGATCAACATTGAGCTGTATTGTGTGGGTAAGAGCGCAAAAGGAGGAGAAGACCTTGGCATTCTCGGGCGTTTCTGGAGCGGCATTGAGAGCCTGTTCTTCACCAATGTCAACAAGCAAACGGACACCGTCACCAAAGAAGTCCAGAAGCTGCTTTCCTACAAAAATGAGAGTGGATGGGCTGTGCTTAGCAAAGGGTCTACTGTGGTGGTCACTGGTCACGGCTTCACAATCTTGAAGGTGCTTGAGGACTTTGAGAGATGGAAGGAGTTTATCAAGGAAAAGGGCTTTGAGGTCTCATTCAAAGCGTACCACGAGAAGGTGATTCAGACTATGAAGCACTGTTGCCGCCTTGACATCCCAAGTGTTGCAGGAAAGGTTCCGGAGACAATGAAGTGTCCTGAGTGTCCTCGCACCATGGAGACCTATGTGAGCTACAAGTGTTGCCACACTGATGGTCCCATTAACGCACATCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SEO_C SEO_N

Protein Analysis

683

Amino Acids

78.0

Weight (kDa)

8.21

Isoelectric Point (pI)

38.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SEO_N PF14576 34 - 304 3.5e-86 Sieve element occlusion N-terminus
SEO_C PF14577 476 - 538 4.5e-13 Sieve element occlusion C-terminus
SEO_C PF14577 548 - 674 2.1e-35 Sieve element occlusion C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000511)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g02370 FvH4_4g02370 FvH4_4g02370 FvH4_4g02430 FvH4_4g02430 FvH4_4g02460 FvH4_4g02460 FvH4_4g02460 FvH4_4g02480 FvH4_4g02480 FvH4_4g02480 FvH4_4g02480 FvH4_6g05920
malus_domestica MD00G1111000.v1.1 MD08G1225800.v1.1 MD13G1205700.v1.1 MD13G1205800.v1.1 MD13G1205900.v1.1 MD13G1206000.v1.1 MD16G1207500.v1.1 MD16G1207600.v1.1
prunus_persica Prupe.1G028400_v2.0.a1 Prupe.1G028500_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028900_v2.0.a1 Prupe.1G029000_v2.0.a1 Prupe.1G029100_v2.0.a1
pyrus_communis pycom08g19680 pycom08g19730 pycom16g17460 pycom16g17500
rosa_chinensis RchiOBHm_Chr4g0389751 RchiOBHm_Chr4g0389771 RchiOBHm_Chr4g0389791 RchiOBHm_Chr4g0389861 RchiOBHm_Chr4g0389881 RchiOBHm_Chr4g0389901 RchiOBHm_Chr4g0412411
rosa_laevigata RLG00000008288 RLG00000009901 RLG00000009945 RLG00000009954 RLG00000009956 RLG00000009957 RLG00000009958 RLG00000019246
rosa_multiflora Rmu_co8275125.1_g000001 Rmu_sc0002083.1_g000003 Rmu_sc0002083.1_g000025 Rmu_sc0002604.1_g000016 Rmu_sc0004336.1_g000031 Rmu_sc0010423.1_g000003
rosa_roxburghii Rroxscaffold_5G00335790 Rroxscaffold_5G00335850 Rroxscaffold_5G00335970 Rroxscaffold_5G00335990
rosa_rugosa Rorug03G0325100 Rorug03G0325300 Rorug03G0325500.1 Rorug03G0326800 Rorug03G0327000 Rorug04G0104400
rosa_samantha Rh4AG029900 Rh4AG030000 Rh4AG030100 Rh4AG030200 Rh4AG030600 Rh4AG172900 Rh4BG023200 Rh4BG023900 Rh4BG024000 Rh4BG024100 Rh4BG172200 Rh4DG023300 Rh4DG023800 Rh4DG023900 Rh4DG024000 Rh4DG151600
rosa_wichuraiana Rw1G002330 Rw1G002340 Rw4G002260 Rw4G002270 Rw4G002280 Rw4G002300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1056
AccBSI CCGCTC 1 cut(s) 1653
AccI GTMKAC 1 cut(s) 1772
AccII CGCG 1 cut(s) 480
AccIII TCCGGA 1 cut(s) 1954
AciI CCGC 2 cut(s) 1653, 1921
AclWI GGATC 5 cut(s) 696, 896, 1081, 1469, 1547
AcsI RAATTY 4 cut(s) 312, 455, 1172, 1261
AcuI CTGAAG 1 cut(s) 1205
AcyI GRCGYC 1 cut(s) 1053
AdeI CACNNNGTG 1 cut(s) 22
AfaI GTAC 5 cut(s) 235, 291, 1118, 1217, 1883
AfiI CCNNNNNNNGG 3 cut(s) 72, 1232, 1891
AflII CTTAAG 2 cut(s) 305, 1571
AgsI TTSAA 7 cut(s) 634, 934, 984, 1267, 1433, 1810, 1876
AhlI ACTAGT 1 cut(s) 761
AjnI CCWGG 2 cut(s) 340, 465
AjuI GAANNNNNNNTTGG 2 cut(s) 266, 298
AleI CACNNNNGTG 1 cut(s) 368
Alw21I GWGCWC 2 cut(s) 302, 1455
Alw26I GTCTC 6 cut(s) 869, 1060, 1101, 1873, 1952, 1988
Alw44I GTGCAC 2 cut(s) 298, 1451
AlwI GGATC 5 cut(s) 696, 896, 1081, 1469, 1547
AlwNI CAGNNNCTG 1 cut(s) 1723
Ama87I CYCGRG 1 cut(s) 1637
Aor13HI TCCGGA 1 cut(s) 1954
AoxI GGCC 3 cut(s) 7, 597, 1356
ApaLI GTGCAC 2 cut(s) 298, 1451
ApeKI GCWGC 5 cut(s) 431, 472, 550, 1210, 1723
ApoI RAATTY 4 cut(s) 312, 455, 1172, 1261
Asp700I GAANNNNTTC 2 cut(s) 899, 1512
AspLEI GCGC 3 cut(s) 472, 561, 1610
AspS9I GGNCC 2 cut(s) 1223, 2030
AsuHPI GGTGA 7 cut(s) 356, 464, 1300, 1433, 1666, 1696, 1906
AvaI CYCGRG 1 cut(s) 1637
AvaII GGWCC 2 cut(s) 1223, 2030
AxyI CCTNAGG 1 cut(s) 1013
BaeGI GKGCMC 2 cut(s) 302, 1455
BaeI ACNNNNGTAYC 2 cut(s) 225, 258
BarI GAAGNNNNNNTAC 2 cut(s) 1469, 1501
BauI CACGAG 2 cut(s) 1091, 1886
BbsI GAAGAC 1 cut(s) 1629
Bbv12I GWGCWC 2 cut(s) 302, 1455
BbvI GCAGC 5 cut(s) 443, 484, 537, 1197, 1710
BccI CCATC 5 cut(s) 971, 1453, 1743, 1827, 2021
BceAI ACGGC 2 cut(s) 526, 1810
BcgI CGANNNNNNTGC 2 cut(s) 1319, 1353
BciT130I CCWGG 2 cut(s) 342, 467
BclI TGATCA 1 cut(s) 591
BcoDI GTCTC 6 cut(s) 869, 1060, 1101, 1873, 1952, 1988
BcuI ACTAGT 1 cut(s) 761
BfaI CTAG 6 cut(s) 5, 11, 428, 762, 788, 1049
BfmI CTRYAG 1 cut(s) 1211
BfrI CTTAAG 2 cut(s) 305, 1571
BisI GCNGC 7 cut(s) 432, 473, 551, 1211, 1654, 1724, 1921
BlpI GCTNAGC 2 cut(s) 80, 1760
BlsI GCNGC 7 cut(s) 433, 474, 552, 1212, 1655, 1725, 1922
BmcAI AGTACT 1 cut(s) 1217
Bme1390I CCNGG 2 cut(s) 342, 467
Bme18I GGWCC 2 cut(s) 1223, 2030
BmeT110I CYCGRG 1 cut(s) 1637
BmgT120I GGNCC 2 cut(s) 1223, 2030
BmiI GGNNCC 5 cut(s) 464, 1351, 1423, 1953, 2032
BmrFI CCNGG 2 cut(s) 342, 467
BmsI GCATC 4 cut(s) 315, 425, 630, 1245
BpiI GAAGAC 1 cut(s) 1629
BpmI CTGGAG 1 cut(s) 1669
Bpu1102I GCTNAGC 2 cut(s) 80, 1760
BpuEI CTTGAG 5 cut(s) 179, 222, 521, 596, 1838
BsaBI GATNNNNATC 1 cut(s) 1007
BsaHI GRCGYC 1 cut(s) 1053
BsaI GGTCTC 2 cut(s) 1873, 1988
BsaJI CCNNGG 7 cut(s) 30, 39, 684, 1199, 1353, 1627, 1989
BsaWI WCCGGW 2 cut(s) 697, 1954
BsaXI ACNNNNNCTCC 2 cut(s) 1460, 1490
Bsc4I CCNNNNNNNGG 3 cut(s) 72, 1232, 1891
Bse1I ACTGG 3 cut(s) 279, 628, 1792
Bse21I CCTNAGG 1 cut(s) 1013
Bse3DI GCAATG 4 cut(s) 66, 335, 1154, 1542
Bse8I GATNNNNATC 1 cut(s) 1007
BseAI TCCGGA 1 cut(s) 1954
BseBI CCWGG 2 cut(s) 342, 467
BseDI CCNNGG 7 cut(s) 30, 39, 684, 1199, 1353, 1627, 1989
BseGI GGATG 4 cut(s) 189, 1008, 1754, 1929
BseJI GATNNNNATC 1 cut(s) 1007
BseLI CCNNNNNNNGG 3 cut(s) 72, 1232, 1891
BseMI GCAATG 4 cut(s) 66, 335, 1154, 1542
BseMII CTCAG 3 cut(s) 71, 1154, 1965
BseNI ACTGG 3 cut(s) 279, 628, 1792
BseRI GAGGAG 7 cut(s) 131, 275, 524, 881, 1268, 1484, 1632
BseSI GKGCMC 2 cut(s) 302, 1455
BseXI GCAGC 5 cut(s) 443, 484, 537, 1197, 1710
BseYI CCCAGC 1 cut(s) 71
BsgI GTGCAG 3 cut(s) 248, 536, 1313
Bsh1236I CGCG 1 cut(s) 480
BshFI GGCC 3 cut(s) 9, 599, 1358
BsiHKAI GWGCWC 2 cut(s) 302, 1455
BsiHKCI CYCGRG 1 cut(s) 1637
BsiSI CCGG 2 cut(s) 698, 1955
BslFI GGGAC 4 cut(s) 509, 650, 951, 2016
BslI CCNNNNNNNGG 3 cut(s) 72, 1232, 1891
BsmAI GTCTC 6 cut(s) 869, 1060, 1101, 1873, 1952, 1988
BsmBI CGTCTC 1 cut(s) 1060
BsmFI GGGAC 4 cut(s) 509, 650, 951, 2016
BsmI GAATGC 2 cut(s) 623, 1632
BsnI GGCC 3 cut(s) 9, 599, 1358
Bso31I GGTCTC 2 cut(s) 1873, 1988
BsoBI CYCGRG 1 cut(s) 1637
Bsp1286I GDGCHC 2 cut(s) 302, 1455
Bsp13I TCCGGA 1 cut(s) 1954
Bsp1720I GCTNAGC 2 cut(s) 80, 1760
Bsp19I CCATGG 1 cut(s) 1989
Bsp68I TCGCGA 1 cut(s) 480
BspACI CCGC 2 cut(s) 1653, 1921
BspANI GGCC 3 cut(s) 9, 599, 1358
BspCNI CTCAG 3 cut(s) 70, 1155, 1966
BspEI TCCGGA 1 cut(s) 1954
BspFNI CGCG 1 cut(s) 480
BspLI GGNNCC 5 cut(s) 464, 1351, 1423, 1953, 2032
BspMAI CTGCAG 1 cut(s) 1215
BspPI GGATC 5 cut(s) 696, 896, 1081, 1469, 1547
BspTI CTTAAG 2 cut(s) 305, 1571
BspTNI GGTCTC 2 cut(s) 1873, 1988
BsrBI CCGCTC 1 cut(s) 1653
BsrDI GCAATG 4 cut(s) 66, 335, 1154, 1542
BsrI ACTGG 3 cut(s) 279, 628, 1792
BssECI CCNNGG 7 cut(s) 30, 39, 684, 1199, 1353, 1627, 1989
BssNI GRCGYC 1 cut(s) 1053
BssSI CACGAG 2 cut(s) 1091, 1886
BssT1I CCWWGG 6 cut(s) 30, 684, 1199, 1353, 1627, 1989
Bst2BI CACGAG 2 cut(s) 1091, 1886
Bst2UI CCWGG 2 cut(s) 342, 467
Bst4CI ACNGT 8 cut(s) 370, 727, 997, 1282, 1392, 1702, 1777, 1916
BstACI GRCGYC 1 cut(s) 1053
BstAFI CTTAAG 2 cut(s) 305, 1571
BstAPI GCANNNNNTGC 1 cut(s) 437
BstC8I GCNNGC 5 cut(s) 201, 302, 335, 563, 1234
BstDEI CTNAG 9 cut(s) 57, 80, 744, 842, 987, 1013, 1163, 1760, 1974
BstDSI CCRYGG 1 cut(s) 1989
BstENI CCTNNNNNAGG 1 cut(s) 1230
BstF5I GGATG 4 cut(s) 189, 1008, 1754, 1929
BstFNI CGCG 1 cut(s) 480
BstHHI GCGC 3 cut(s) 472, 561, 1610
BstMAI GTCTC 6 cut(s) 869, 1060, 1101, 1873, 1952, 1988
BstMWI GCNNNNNNNGC 6 cut(s) 334, 437, 469, 547, 556, 1662
BstNI CCWGG 2 cut(s) 342, 467
BstNSI RCATGY 1 cut(s) 248
BstSCI CCNGG 2 cut(s) 340, 465
BstSFI CTRYAG 1 cut(s) 1211
BstSLI GKGCMC 2 cut(s) 302, 1455
BstUI CGCG 1 cut(s) 480
BstV1I GCAGC 5 cut(s) 443, 484, 537, 1197, 1710
BstV2I GAAGAC 1 cut(s) 1629
BstXI CCANNNNNNTGG 1 cut(s) 2027
Bsu36I CCTNAGG 1 cut(s) 1013
BsuRI GGCC 3 cut(s) 9, 599, 1358
BtgI CCRYGG 1 cut(s) 1989
BtsCI GGATG 4 cut(s) 189, 1008, 1754, 1929
BtsI GCAGTG 1 cut(s) 602
BtsIMutI CAGTG 7 cut(s) 375, 419, 602, 723, 1785, 1912, 2022
BtuMI TCGCGA 1 cut(s) 480
Cac8I GCNNGC 5 cut(s) 201, 302, 335, 563, 1234
CaiI CAGNNNCTG 1 cut(s) 1723
CfoI GCGC 3 cut(s) 472, 561, 1610
Cfr13I GGNCC 2 cut(s) 1223, 2030
Csp6I GTAC 5 cut(s) 234, 290, 1117, 1216, 1882
CviAII CATG 7 cut(s) 94, 134, 245, 402, 1333, 1403, 1990
CviQI GTAC 5 cut(s) 234, 290, 1117, 1216, 1882
DdeI CTNAG 9 cut(s) 57, 80, 744, 842, 987, 1013, 1163, 1760, 1974
DraIII CACNNNGTG 1 cut(s) 22
Eco130I CCWWGG 6 cut(s) 30, 684, 1199, 1353, 1627, 1989
Eco147I AGGCCT 2 cut(s) 9, 1358
Eco31I GGTCTC 2 cut(s) 1873, 1988
Eco32I GATATC 1 cut(s) 1180
Eco47I GGWCC 2 cut(s) 1223, 2030
Eco57I CTGAAG 1 cut(s) 1205
Eco81I CCTNAGG 1 cut(s) 1013
Eco88I CYCGRG 1 cut(s) 1637
EcoNI CCTNNNNNAGG 1 cut(s) 1230
EcoRI GAATTC 1 cut(s) 455
EcoRII CCWGG 2 cut(s) 340, 465
EcoRV GATATC 1 cut(s) 1180
EcoT14I CCWWGG 6 cut(s) 30, 684, 1199, 1353, 1627, 1989
EcoT22I ATGCAT 1 cut(s) 623
ErhI CCWWGG 6 cut(s) 30, 684, 1199, 1353, 1627, 1989
Esp3I CGTCTC 1 cut(s) 1060
FaeI CATG 7 cut(s) 97, 137, 248, 405, 1336, 1406, 1993
FaqI GGGAC 4 cut(s) 509, 650, 951, 2016
FatI CATG 7 cut(s) 93, 133, 244, 401, 1332, 1402, 1989
FbaI TGATCA 1 cut(s) 591
FblI GTMKAC 1 cut(s) 1772
Fnu4HI GCNGC 7 cut(s) 432, 473, 551, 1211, 1654, 1724, 1921
FokI GGATG 4 cut(s) 176, 995, 1761, 1916
Fsp4HI GCNGC 7 cut(s) 432, 473, 551, 1211, 1654, 1724, 1921
FspBI CTAG 6 cut(s) 5, 11, 428, 762, 788, 1049
GlaI GCGC 3 cut(s) 471, 560, 1609
GluI GCNGC 7 cut(s) 432, 473, 551, 1211, 1654, 1724, 1921
GsaI CCCAGC 1 cut(s) 75
GsuI CTGGAG 1 cut(s) 1669
HaeIII GGCC 3 cut(s) 9, 599, 1358
HapII CCGG 2 cut(s) 698, 1955
HhaI GCGC 3 cut(s) 472, 561, 1610
Hin1I GRCGYC 1 cut(s) 1053
Hin1II CATG 7 cut(s) 97, 137, 248, 405, 1336, 1406, 1993
Hin6I GCGC 3 cut(s) 470, 559, 1608
HinP1I GCGC 3 cut(s) 470, 559, 1608
HincII GTYRAC 1 cut(s) 1684
HindII GTYRAC 1 cut(s) 1684
HpaII CCGG 2 cut(s) 698, 1955
HphI GGTGA 7 cut(s) 356, 464, 1300, 1433, 1666, 1696, 1906
Hpy166II GTNNAC 9 cut(s) 49, 139, 250, 300, 1098, 1206, 1453, 1684, 1773
Hpy188I TCNGA 8 cut(s) 60, 487, 547, 591, 905, 1060, 1080, 1902
Hpy8I GTNNAC 9 cut(s) 49, 139, 250, 300, 1098, 1206, 1453, 1684, 1773
HpyAV CCTTC 6 cut(s) 879, 1369, 1427, 1804, 1831, 1885
HpyCH4III ACNGT 8 cut(s) 370, 727, 997, 1282, 1392, 1702, 1777, 1916
HpyCH4IV ACGT 1 cut(s) 1053
HpyF10VI GCNNNNNNNGC 6 cut(s) 334, 437, 469, 547, 556, 1662
HpyF3I CTNAG 9 cut(s) 57, 80, 744, 842, 987, 1013, 1163, 1760, 1974
HpySE526I ACGT 1 cut(s) 1053
Hsp92I GRCGYC 1 cut(s) 1053
Hsp92II CATG 7 cut(s) 97, 137, 248, 405, 1336, 1406, 1993
HspAI GCGC 3 cut(s) 470, 559, 1608
Kpn2I TCCGGA 1 cut(s) 1954
Ksp22I TGATCA 1 cut(s) 591
LmnI GCTCC 6 cut(s) 76, 468, 1144, 1349, 1427, 1650
Lsp1109I GCAGC 5 cut(s) 443, 484, 537, 1197, 1710
LweI GCATC 4 cut(s) 315, 425, 630, 1245
MaeI CTAG 6 cut(s) 5, 11, 428, 762, 788, 1049
MaeII ACGT 1 cut(s) 1053
MaeIII GTNAC 5 cut(s) 344, 1392, 1702, 1783, 1790
MbiI CCGCTC 1 cut(s) 1653
MboII GAAGA 7 cut(s) 118, 1198, 1394, 1397, 1477, 1634, 1663
MfeI CAATTG 2 cut(s) 504, 1137
MhlI GDGCHC 2 cut(s) 302, 1455
MlyI GAGTC 1 cut(s) 531
MmeI TCCRAC 1 cut(s) 1344
Mph1103I ATGCAT 1 cut(s) 623
MroI TCCGGA 1 cut(s) 1954
MroXI GAANNNNTTC 2 cut(s) 899, 1512
MseI TTAA 6 cut(s) 306, 582, 999, 1563, 1572, 2037
MslI CAYNNNNRTG 5 cut(s) 144, 368, 746, 1401, 2025
MspCI CTTAAG 2 cut(s) 305, 1571
MspI CCGG 2 cut(s) 698, 1955
MspR9I CCNGG 2 cut(s) 342, 467
MunI CAATTG 2 cut(s) 504, 1137
Mva1269I GAATGC 2 cut(s) 623, 1632
MvaI CCWGG 2 cut(s) 342, 467
MvnI CGCG 1 cut(s) 480
MwoI GCNNNNNNNGC 6 cut(s) 334, 437, 469, 547, 556, 1662
NcoI CCATGG 1 cut(s) 1989
NlaIII CATG 7 cut(s) 97, 137, 248, 405, 1336, 1406, 1993
NlaIV GGNNCC 5 cut(s) 464, 1351, 1423, 1953, 2032
NmuCI GTSAC 5 cut(s) 344, 1392, 1702, 1783, 1790
NruI TCGCGA 1 cut(s) 480
NsiI ATGCAT 1 cut(s) 623
NspI RCATGY 1 cut(s) 248
OliI CACNNNNGTG 1 cut(s) 368
PceI AGGCCT 2 cut(s) 9, 1358
PcsI WCGNNNNNNNCGW 2 cut(s) 621, 1059
PctI GAATGC 2 cut(s) 623, 1632
PdmI GAANNNNTTC 2 cut(s) 899, 1512
PfeI GAWTC 9 cut(s) 149, 164, 482, 835, 1075, 1132, 1336, 1513, 1897
PflFI GACNNNGTC 1 cut(s) 1700
PkrI GCNGC 7 cut(s) 433, 474, 552, 1212, 1655, 1725, 1922
PleI GAGTC 1 cut(s) 530
PpsI GAGTC 1 cut(s) 530
Psp6I CCWGG 2 cut(s) 340, 465
PspFI CCCAGC 1 cut(s) 71
PspGI CCWGG 2 cut(s) 340, 465
PspN4I GGNNCC 5 cut(s) 464, 1351, 1423, 1953, 2032
PspPI GGNCC 2 cut(s) 1223, 2030
PstI CTGCAG 1 cut(s) 1215
PstNI CAGNNNCTG 1 cut(s) 1723
PsyI GACNNNGTC 1 cut(s) 1700
RruI TCGCGA 1 cut(s) 480
RsaI GTAC 5 cut(s) 235, 291, 1118, 1217, 1883
RsaNI GTAC 5 cut(s) 234, 290, 1117, 1216, 1882
RseI CAYNNNNRTG 5 cut(s) 144, 368, 746, 1401, 2025
SaqAI TTAA 6 cut(s) 306, 582, 999, 1563, 1572, 2037
SatI GCNGC 7 cut(s) 432, 473, 551, 1211, 1654, 1724, 1921
Sau96I GGNCC 2 cut(s) 1223, 2030
ScaI AGTACT 1 cut(s) 1217
SchI GAGTC 1 cut(s) 531
ScrFI CCNGG 2 cut(s) 342, 467
SduI GDGCHC 2 cut(s) 302, 1455
SfaNI GCATC 4 cut(s) 315, 425, 630, 1245
SfcI CTRYAG 1 cut(s) 1211
SinI GGWCC 2 cut(s) 1223, 2030
SmiMI CAYNNNNRTG 5 cut(s) 144, 368, 746, 1401, 2025
SmlI CTYRAG 7 cut(s) 194, 237, 305, 536, 575, 1571, 1817
SmoI CTYRAG 7 cut(s) 194, 237, 305, 536, 575, 1571, 1817
SpeI ACTAGT 1 cut(s) 761
SseBI AGGCCT 2 cut(s) 9, 1358
SsiI CCGC 2 cut(s) 1653, 1921
SspMI CTAG 6 cut(s) 5, 11, 428, 762, 788, 1049
StuI AGGCCT 2 cut(s) 9, 1358
StyD4I CCNGG 2 cut(s) 340, 465
StyI CCWWGG 6 cut(s) 30, 684, 1199, 1353, 1627, 1989
TaaI ACNGT 8 cut(s) 370, 727, 997, 1282, 1392, 1702, 1777, 1916
TaiI ACGT 1 cut(s) 1056
TaqI TCGA 7 cut(s) 156, 615, 624, 677, 1005, 1073, 1311
TatI WGTACW 2 cut(s) 1116, 1215
TauI GCSGC 2 cut(s) 1656, 1923
TfiI GAWTC 9 cut(s) 149, 164, 482, 835, 1075, 1132, 1336, 1513, 1897
Tru1I TTAA 6 cut(s) 306, 582, 999, 1563, 1572, 2037
Tru9I TTAA 6 cut(s) 306, 582, 999, 1563, 1572, 2037
TscAI CASTG 7 cut(s) 375, 426, 609, 730, 1792, 1919, 2029
TseFI GTSAC 5 cut(s) 344, 1392, 1702, 1783, 1790
TseI GCWGC 5 cut(s) 431, 472, 550, 1210, 1723
Tsp45I GTSAC 5 cut(s) 344, 1392, 1702, 1783, 1790
TspDTI ATGAA 8 cut(s) 162, 390, 1027, 1234, 1307, 1521, 1922, 1979
TspGWI ACGGA 3 cut(s) 1414, 1506, 1709
TspRI CASTG 7 cut(s) 375, 426, 609, 730, 1792, 1919, 2029
Tth111I GACNNNGTC 1 cut(s) 1700
Vha464I CTTAAG 2 cut(s) 305, 1571
VneI GTGCAC 2 cut(s) 298, 1451
VpaK11BI GGWCC 2 cut(s) 1223, 2030
XagI CCTNNNNNAGG 1 cut(s) 1230
XapI RAATTY 4 cut(s) 312, 455, 1172, 1261
XceI RCATGY 1 cut(s) 248
XmiI GTMKAC 1 cut(s) 1772
XmnI GAANNNNTTC 2 cut(s) 899, 1512
XspI CTAG 6 cut(s) 5, 11, 428, 762, 788, 1049
ZraI GACGTC 1 cut(s) 1054
ZrmI AGTACT 1 cut(s) 1217
Zsp2I ATGCAT 1 cut(s) 623
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.