Rh4DG151600

Sieve element occlusion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000511
Physical Location & Seq
Reverse (-)
30220431 .. 30223778
3348 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG151600.1

Sequence Viewer

Length: 1524 bp
ATGATGCTAAGCCAAGCAAACAATGTGGCTACCACGATGGCCTCAGTAATCCACACTACTCATAAAGTAATCGAAACTACTCTGGAAACCATTCTTGGGGAGTCTAGCCCATTTGCCATGTCTGACGAGAAGATCTTGGAAGAAATTTACACTACTCATGTCCATGTTGATGACTCCTTTGACGACGATTCTCTTTTCGTCATTGTTGAGAACATTCTTAAGCATGCAATCCAAACTGTTGACGAAATTGTGCAGCGTACTGAAGTGCATGAGGAAAACATAGAGGAGAAGACCCCCAAAGCCAGCTTCAGGGTACCAGTGTGCACACTTAAGGGAGTCTCCTTCGAGATGCAATGCAAGCCACCTGGTGAGGAAATTGCCCACAAGACAACACTGGCAATACTCAACAAGCTGTCAAAGTATTCATGGGAAGCAAAGGCATCATTGACCCTAGCAGCTTTTGCTATGGAATATGGTCAATTCTGGCTCCTTGCTCGGCTTATTCGCCAATCTGACCACCTTGCCAAATCAATTGCAATCCTCAATGGAGTCCCCATACTCCTCAAGCCATCGGAGCCGAAGAAGCGCAAGCAATCAATTCTTGAGCTTAACAATCTGATCAAGGAAACACTGCAAGTGATCGAATTAATCGACCAGTTCAACAAGCTTTTGGTTCATCACCCAAAAGATGTGCCCGATTGGCTTAAAGCATTGGATCATATTCCAGTGGATGTCTATTGGGCTATCATGGCTATTGTGGCTTGTGCTACTAAGGTCACTATACTCACTAGTGATGAGGATAAGGAATTTGATCTATCTCCCTATTCTGAAAAAATCCACTATGTCCATAAGGACCTCGATGGACGGCTAAGACGTTGCAGAAAACAAATTGAGGAGGCAGAGATTTACAGAAGGCTCATAAAAATGGTCCACACCCCTACTGAAATTATGGAGGTTTTAAAAGTATTGATATTTACAAAGGATAGTGTCCAGCCACTCATCGATGGTTTAACTAAGCAAACGGTTAACATTGACATCATAAGGAGGAAGAATGTGTTATTGTTCATTTCCAGCTTAGACATTACGGATGACGACATTTCGGTTCTGAAACGGATCCATGAGTTCACAAAGATCAATCAGTATAAGATTGTCTGGATACCAATTGAGGAGCAATGGACTGACGACCTGAGAAAGAAGTTTGATGACCTGAAGAACAATAAGCATCTGCCATGGTTTACGGTGCAGTACTCTGGACGTATAGCAGGCATCAAGTTCATCAAGGAGGAGTGGAAGTTCAAGGGTAAGCCTACAGTGGTGGTGATGAACCCACAAGGAAAGGTCGAACACCCTAACGCTTTCCACATGATTCGAGTATGGGGAGAAGAGGCCTTCCCTTTCAGCAAGACAAGAGAAAAAGAACTGTCATATTCTATTAAAAAAAATTGGGTTGTCTCTGTTGTTGAAGGAATTCATCCGTCTGTGCTCACTTGGGTAATTCTCTTCAACTTATCAGTGTGCATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SEO_C SEO_N

Protein Analysis

507

Amino Acids

58.53

Weight (kDa)

6.46

Isoelectric Point (pI)

46.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SEO_N PF14576 41 - 313 1.5e-76 Sieve element occlusion N-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000511)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g02370 FvH4_4g02370 FvH4_4g02370 FvH4_4g02430 FvH4_4g02430 FvH4_4g02460 FvH4_4g02460 FvH4_4g02460 FvH4_4g02480 FvH4_4g02480 FvH4_4g02480 FvH4_4g02480 FvH4_6g05920
malus_domestica MD00G1111000.v1.1 MD08G1225800.v1.1 MD13G1205700.v1.1 MD13G1205800.v1.1 MD13G1205900.v1.1 MD13G1206000.v1.1 MD16G1207500.v1.1 MD16G1207600.v1.1
prunus_persica Prupe.1G028400_v2.0.a1 Prupe.1G028500_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028900_v2.0.a1 Prupe.1G029000_v2.0.a1 Prupe.1G029100_v2.0.a1
pyrus_communis pycom08g19680 pycom08g19730 pycom16g17460 pycom16g17500
rosa_chinensis RchiOBHm_Chr4g0389751 RchiOBHm_Chr4g0389771 RchiOBHm_Chr4g0389791 RchiOBHm_Chr4g0389861 RchiOBHm_Chr4g0389881 RchiOBHm_Chr4g0389901 RchiOBHm_Chr4g0412411
rosa_laevigata RLG00000008288 RLG00000009901 RLG00000009945 RLG00000009954 RLG00000009956 RLG00000009957 RLG00000009958 RLG00000019246
rosa_multiflora Rmu_co8275125.1_g000001 Rmu_sc0002083.1_g000003 Rmu_sc0002083.1_g000025 Rmu_sc0002604.1_g000016 Rmu_sc0004336.1_g000031 Rmu_sc0010423.1_g000003
rosa_roxburghii Rroxscaffold_5G00335790 Rroxscaffold_5G00335850 Rroxscaffold_5G00335970 Rroxscaffold_5G00335990
rosa_rugosa Rorug03G0325100 Rorug03G0325300 Rorug03G0325500.1 Rorug03G0326800 Rorug03G0327000 Rorug04G0104400
rosa_samantha Rh4AG029900 Rh4AG030000 Rh4AG030100 Rh4AG030200 Rh4AG030600 Rh4AG172900 Rh4BG023200 Rh4BG023900 Rh4BG024000 Rh4BG024100 Rh4BG172200 Rh4DG023300 Rh4DG023800 Rh4DG023900 Rh4DG024000 Rh4DG151600
rosa_wichuraiana Rw1G002330 Rw1G002340 Rw4G002260 Rw4G002270 Rw4G002280 Rw4G002300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 313
AccB1I GGYRCC 1 cut(s) 313
AclWI GGATC 3 cut(s) 723, 1108, 1121
AcsI RAATTY 3 cut(s) 144, 806, 1467
AcuI CTGAAG 3 cut(s) 282, 292, 1229
AdeI CACNNNGTG 1 cut(s) 368
AfaI GTAC 3 cut(s) 259, 315, 1247
AfiI CCNNNNNNNGG 2 cut(s) 96, 309
AflII CTTAAG 2 cut(s) 218, 329
AgsI TTSAA 4 cut(s) 661, 1297, 1463, 1504
AhlI ACTAGT 1 cut(s) 788
AjnI CCWGG 1 cut(s) 364
AjuI GAANNNNNNNTTGG 4 cut(s) 78, 110, 290, 322
AloI GAACNNNNNNTCC 2 cut(s) 1277, 1309
AluBI AGCT 6 cut(s) 306, 412, 458, 607, 667, 1074
AluI AGCT 6 cut(s) 306, 412, 458, 607, 667, 1074
Alw21I GWGCWC 2 cut(s) 326, 1485
Alw26I GTCTC 2 cut(s) 343, 1456
Alw44I GTGCAC 1 cut(s) 322
AlwI GGATC 3 cut(s) 723, 1108, 1121
AoxI GGCC 2 cut(s) 39, 1386
ApaLI GTGCAC 1 cut(s) 322
ApeKI GCWGC 2 cut(s) 253, 455
ApoI RAATTY 3 cut(s) 144, 806, 1467
AseI ATTAAT 1 cut(s) 647
Asp700I GAANNNNTTC 2 cut(s) 90, 1467
Asp718I GGTACC 1 cut(s) 313
AspLEI GCGC 1 cut(s) 588
AspS9I GGNCC 2 cut(s) 853, 928
AsuHPI GGTGA 3 cut(s) 380, 671, 1330
AvaII GGWCC 2 cut(s) 853, 928
BaeGI GKGCMC 2 cut(s) 326, 696
BaeI ACNNNNGTAYC 2 cut(s) 305, 338
BamHI GGATCC 1 cut(s) 1113
BanI GGYRCC 1 cut(s) 313
BbsI GAAGAC 1 cut(s) 296
Bbv12I GWGCWC 2 cut(s) 326, 1485
BbvI GCAGC 2 cut(s) 265, 467
BccI CCATC 4 cut(s) 31, 577, 854, 998
BceAI ACGGC 1 cut(s) 881
BciT130I CCWGG 1 cut(s) 366
BciVI GTATCC 1 cut(s) 1149
BclI TGATCA 1 cut(s) 618
BcoDI GTCTC 2 cut(s) 343, 1456
BcuI ACTAGT 1 cut(s) 788
BfaI CTAG 3 cut(s) 105, 452, 789
BfmI CTRYAG 1 cut(s) 1308
BfrI CTTAAG 2 cut(s) 218, 329
BfuI GTATCC 1 cut(s) 1149
BglI GCCNNNNNGGC 1 cut(s) 700
BglII AGATCT 1 cut(s) 132
BisI GCNGC 2 cut(s) 254, 456
BlpI GCTNAGC 1 cut(s) 8
BlsI GCNGC 2 cut(s) 255, 457
BmcAI AGTACT 1 cut(s) 1247
Bme1390I CCNGG 1 cut(s) 366
Bme18I GGWCC 2 cut(s) 853, 928
BmgT120I GGNCC 2 cut(s) 853, 928
BmiI GGNNCC 4 cut(s) 315, 488, 576, 1115
BmrFI CCNGG 1 cut(s) 366
BmsI GCATC 4 cut(s) 339, 449, 1231, 1275
BpiI GAAGAC 1 cut(s) 296
Bpu1102I GCTNAGC 1 cut(s) 8
BpuEI CTTGAG 2 cut(s) 548, 623
Bsa29I ATCGAT 1 cut(s) 1002
BsaJI CCNNGG 1 cut(s) 1229
BsaXI ACNNNNNCTCC 2 cut(s) 1277, 1307
Bsc4I CCNNNNNNNGG 2 cut(s) 96, 309
Bse1I ACTGG 4 cut(s) 317, 399, 655, 725
Bse3DI GCAATG 2 cut(s) 359, 1178
BseBI CCWGG 1 cut(s) 366
BseCI ATCGAT 1 cut(s) 1002
BseDI CCNNGG 1 cut(s) 1229
BseGI GGATG 3 cut(s) 736, 1093, 1471
BseLI CCNNNNNNNGG 2 cut(s) 96, 309
BseMI GCAATG 2 cut(s) 359, 1178
BseMII CTCAG 2 cut(s) 57, 1178
BseNI ACTGG 4 cut(s) 317, 399, 655, 725
BseRI GAGGAG 5 cut(s) 299, 551, 908, 1181, 1298
BseSI GKGCMC 2 cut(s) 326, 696
BseXI GCAGC 2 cut(s) 265, 467
BsgI GTGCAG 2 cut(s) 272, 1262
BshFI GGCC 2 cut(s) 41, 1388
BshNI GGYRCC 1 cut(s) 313
BshVI ATCGAT 1 cut(s) 1002
BsiHKAI GWGCWC 2 cut(s) 326, 1485
BslFI GGGAC 1 cut(s) 536
BslI CCNNNNNNNGG 2 cut(s) 96, 309
BsmAI GTCTC 2 cut(s) 343, 1456
BsmFI GGGAC 1 cut(s) 536
BsnI GGCC 2 cut(s) 41, 1388
Bsp1286I GDGCHC 3 cut(s) 326, 696, 1485
Bsp143I GATC 7 cut(s) 132, 618, 639, 715, 811, 1113, 1131
Bsp1720I GCTNAGC 1 cut(s) 8
Bsp19I CCATGG 1 cut(s) 1229
BspANI GGCC 2 cut(s) 41, 1388
BspCNI CTCAG 2 cut(s) 56, 1179
BspDI ATCGAT 1 cut(s) 1002
BspLI GGNNCC 4 cut(s) 315, 488, 576, 1115
BspPI GGATC 3 cut(s) 723, 1108, 1121
BspT107I GGYRCC 1 cut(s) 313
BspTI CTTAAG 2 cut(s) 218, 329
BsrDI GCAATG 2 cut(s) 359, 1178
BsrI ACTGG 4 cut(s) 317, 399, 655, 725
BssECI CCNNGG 1 cut(s) 1229
BssMI GATC 7 cut(s) 132, 618, 639, 715, 811, 1113, 1131
BssT1I CCWWGG 1 cut(s) 1229
Bst2UI CCWGG 1 cut(s) 366
Bst4CI ACNGT 5 cut(s) 238, 1024, 1240, 1312, 1422
Bst6I CTCTTC 2 cut(s) 1377, 1505
BstAFI CTTAAG 2 cut(s) 218, 329
BstAPI GCANNNNNTGC 1 cut(s) 461
BstC8I GCNNGC 5 cut(s) 225, 304, 359, 590, 1264
BstDEI CTNAG 7 cut(s) 8, 43, 771, 869, 1014, 1075, 1187
BstDSI CCRYGG 1 cut(s) 1229
BstF5I GGATG 3 cut(s) 736, 1093, 1471
BstHHI GCGC 1 cut(s) 588
BstKTI GATC 7 cut(s) 135, 621, 642, 718, 814, 1116, 1134
BstMAI GTCTC 2 cut(s) 343, 1456
BstMBI GATC 7 cut(s) 132, 618, 639, 715, 811, 1113, 1131
BstMWI GCNNNNNNNGC 7 cut(s) 358, 461, 574, 583, 700, 749, 758
BstNI CCWGG 1 cut(s) 366
BstNSI RCATGY 1 cut(s) 227
BstSCI CCNGG 1 cut(s) 364
BstSFI CTRYAG 1 cut(s) 1308
BstSLI GKGCMC 2 cut(s) 326, 696
BstV1I GCAGC 2 cut(s) 265, 467
BstV2I GAAGAC 1 cut(s) 296
BstX2I RGATCY 2 cut(s) 132, 1113
BstYI RGATCY 2 cut(s) 132, 1113
Bsu15I ATCGAT 1 cut(s) 1002
BsuI GTATCC 1 cut(s) 1149
BsuRI GGCC 2 cut(s) 41, 1388
BsuTUI ATCGAT 1 cut(s) 1002
BtgI CCRYGG 1 cut(s) 1229
BtsCI GGATG 3 cut(s) 736, 1093, 1471
BtsI GCAGTG 1 cut(s) 629
BtsIMutI CAGTG 6 cut(s) 324, 392, 629, 732, 1317, 1518
Cac8I GCNNGC 5 cut(s) 225, 304, 359, 590, 1264
CfoI GCGC 1 cut(s) 588
Cfr13I GGNCC 2 cut(s) 853, 928
ClaI ATCGAT 1 cut(s) 1002
CsiI ACCWGGT 1 cut(s) 364
Csp6I GTAC 3 cut(s) 258, 314, 1246
CspCI CAANNNNNGTGG 2 cut(s) 6, 41
CviQI GTAC 3 cut(s) 258, 314, 1246
DdeI CTNAG 7 cut(s) 8, 43, 771, 869, 1014, 1075, 1187
DpnI GATC 7 cut(s) 134, 620, 641, 717, 813, 1115, 1133
DpnII GATC 7 cut(s) 132, 618, 639, 715, 811, 1113, 1131
DraI TTTAAA 1 cut(s) 960
DraIII CACNNNGTG 1 cut(s) 368
Eam1104I CTCTTC 2 cut(s) 1377, 1505
EarI CTCTTC 2 cut(s) 1377, 1505
Eco130I CCWWGG 1 cut(s) 1229
Eco147I AGGCCT 1 cut(s) 1388
Eco47I GGWCC 2 cut(s) 853, 928
Eco57I CTGAAG 3 cut(s) 282, 292, 1229
EcoO109I RGGNCCY 1 cut(s) 853
EcoRI GAATTC 1 cut(s) 1467
EcoRII CCWGG 1 cut(s) 364
EcoT14I CCWWGG 1 cut(s) 1229
ErhI CCWWGG 1 cut(s) 1229
FaqI GGGAC 1 cut(s) 536
FbaI TGATCA 1 cut(s) 618
Fnu4HI GCNGC 2 cut(s) 254, 456
FokI GGATG 3 cut(s) 743, 1100, 1458
Fsp4HI GCNGC 2 cut(s) 254, 456
FspBI CTAG 3 cut(s) 105, 452, 789
GlaI GCGC 1 cut(s) 587
GluI GCNGC 2 cut(s) 254, 456
HaeIII GGCC 2 cut(s) 41, 1388
HhaI GCGC 1 cut(s) 588
Hin6I GCGC 1 cut(s) 586
HinP1I GCGC 1 cut(s) 586
HincII GTYRAC 2 cut(s) 241, 1027
HindII GTYRAC 2 cut(s) 241, 1027
HindIII AAGCTT 1 cut(s) 665
HinfI GANTC 6 cut(s) 101, 173, 188, 336, 549, 1366
HpaI GTTAAC 1 cut(s) 1027
HphI GGTGA 3 cut(s) 380, 671, 1330
Hpy166II GTNNAC 6 cut(s) 241, 324, 931, 1027, 1125, 1236
Hpy188I TCNGA 6 cut(s) 124, 514, 574, 618, 829, 1107
Hpy188III TCNNGA 5 cut(s) 83, 346, 602, 1153, 1251
Hpy8I GTNNAC 6 cut(s) 241, 324, 931, 1027, 1125, 1236
Hpy99I CGWCG 1 cut(s) 188
HpyAV CCTTC 4 cut(s) 352, 906, 1399, 1457
HpyCH4III ACNGT 5 cut(s) 238, 1024, 1240, 1312, 1422
HpyCH4IV ACGT 2 cut(s) 874, 1255
HpyF10VI GCNNNNNNNGC 7 cut(s) 358, 461, 574, 583, 700, 749, 758
HpyF3I CTNAG 7 cut(s) 8, 43, 771, 869, 1014, 1075, 1187
HpySE526I ACGT 2 cut(s) 874, 1255
HspAI GCGC 1 cut(s) 586
KpnI GGTACC 1 cut(s) 317
Ksp22I TGATCA 1 cut(s) 618
KspAI GTTAAC 1 cut(s) 1027
Kzo9I GATC 7 cut(s) 132, 618, 639, 715, 811, 1113, 1131
LmnI GCTCC 3 cut(s) 492, 574, 1168
Lsp1109I GCAGC 2 cut(s) 265, 467
LweI GCATC 4 cut(s) 339, 449, 1231, 1275
MabI ACCWGGT 1 cut(s) 364
MaeI CTAG 3 cut(s) 105, 452, 789
MaeII ACGT 2 cut(s) 874, 1255
MaeIII GTNAC 1 cut(s) 775
MalI GATC 7 cut(s) 134, 620, 641, 717, 813, 1115, 1133
MboI GATC 7 cut(s) 132, 618, 639, 715, 811, 1113, 1131
MboII GAAGA 8 cut(s) 142, 152, 301, 592, 1060, 1222, 1394, 1492
MfeI CAATTG 2 cut(s) 531, 1161
MflI RGATCY 2 cut(s) 132, 1113
MhlI GDGCHC 3 cut(s) 326, 696, 1485
MlyI GAGTC 4 cut(s) 110, 167, 345, 558
MroXI GAANNNNTTC 2 cut(s) 90, 1467
MslI CAYNNNNRTG 3 cut(s) 162, 168, 923
MspCI CTTAAG 2 cut(s) 218, 329
MspR9I CCNGG 1 cut(s) 366
MunI CAATTG 2 cut(s) 531, 1161
MvaI CCWGG 1 cut(s) 366
MwoI GCNNNNNNNGC 7 cut(s) 358, 461, 574, 583, 700, 749, 758
NcoI CCATGG 1 cut(s) 1229
NdeII GATC 7 cut(s) 132, 618, 639, 715, 811, 1113, 1131
NlaIV GGNNCC 4 cut(s) 315, 488, 576, 1115
NmeAIII GCCGAG 1 cut(s) 475
NmuCI GTSAC 1 cut(s) 775
NspI RCATGY 1 cut(s) 227
PaeI GCATGC 1 cut(s) 227
PceI AGGCCT 1 cut(s) 1388
PcsI WCGNNNNNNNCGW 2 cut(s) 648, 871
PdmI GAANNNNTTC 2 cut(s) 90, 1467
PfeI GAWTC 2 cut(s) 188, 1366
PkrI GCNGC 2 cut(s) 255, 457
PleI GAGTC 4 cut(s) 109, 167, 344, 557
PpsI GAGTC 4 cut(s) 109, 167, 344, 557
PpuMI RGGWCCY 1 cut(s) 853
PshBI ATTAAT 1 cut(s) 647
Psp5II RGGWCCY 1 cut(s) 853
Psp6I CCWGG 1 cut(s) 364
PspGI CCWGG 1 cut(s) 364
PspN4I GGNNCC 4 cut(s) 315, 488, 576, 1115
PspPI GGNCC 2 cut(s) 853, 928
PspPPI RGGWCCY 1 cut(s) 853
PsuI RGATCY 2 cut(s) 132, 1113
RsaI GTAC 3 cut(s) 259, 315, 1247
RsaNI GTAC 3 cut(s) 258, 314, 1246
RseI CAYNNNNRTG 3 cut(s) 162, 168, 923
SatI GCNGC 2 cut(s) 254, 456
Sau3AI GATC 7 cut(s) 132, 618, 639, 715, 811, 1113, 1131
Sau96I GGNCC 2 cut(s) 853, 928
ScaI AGTACT 1 cut(s) 1247
SchI GAGTC 4 cut(s) 110, 167, 345, 558
ScrFI CCNGG 1 cut(s) 366
SduI GDGCHC 3 cut(s) 326, 696, 1485
SexAI ACCWGGT 1 cut(s) 364
SfaNI GCATC 4 cut(s) 339, 449, 1231, 1275
SfcI CTRYAG 1 cut(s) 1308
SinI GGWCC 2 cut(s) 853, 928
SmiMI CAYNNNNRTG 3 cut(s) 162, 168, 923
SmlI CTYRAG 4 cut(s) 218, 329, 563, 602
SmoI CTYRAG 4 cut(s) 218, 329, 563, 602
SpeI ACTAGT 1 cut(s) 788
SphI GCATGC 1 cut(s) 227
SseBI AGGCCT 1 cut(s) 1388
SspMI CTAG 3 cut(s) 105, 452, 789
StuI AGGCCT 1 cut(s) 1388
StyD4I CCNGG 1 cut(s) 364
StyI CCWWGG 1 cut(s) 1229
TaaI ACNGT 5 cut(s) 238, 1024, 1240, 1312, 1422
TaiI ACGT 2 cut(s) 877, 1258
TaqI TCGA 8 cut(s) 72, 345, 642, 651, 858, 1002, 1341, 1369
TatI WGTACW 1 cut(s) 1245
TfiI GAWTC 2 cut(s) 188, 1366
TscAI CASTG 6 cut(s) 324, 399, 636, 732, 1317, 1518
TseFI GTSAC 1 cut(s) 775
TseI GCWGC 2 cut(s) 253, 455
Tsp45I GTSAC 1 cut(s) 775
TspDTI ATGAA 6 cut(s) 414, 665, 1054, 1264, 1337, 1460
TspGWI ACGGA 3 cut(s) 1100, 1126, 1464
TspRI CASTG 6 cut(s) 324, 399, 636, 732, 1317, 1518
Vha464I CTTAAG 2 cut(s) 218, 329
VneI GTGCAC 1 cut(s) 322
VpaK11BI GGWCC 2 cut(s) 853, 928
VspI ATTAAT 1 cut(s) 647
XapI RAATTY 3 cut(s) 144, 806, 1467
XceI RCATGY 1 cut(s) 227
XmnI GAANNNNTTC 2 cut(s) 90, 1467
XspI CTAG 3 cut(s) 105, 452, 789
ZrmI AGTACT 1 cut(s) 1247
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.