FvH4_6g05920

Sieve element occlusion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000511
Physical Location & Seq
Forward (+)
3374909 .. 3378300
3392 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g05920.t1

Sequence Viewer

Length: 2013 bp
ATGCCTGGCCAAGAAAACAAGGAGGCCTCAGTAATCCACCACCCTCATAGCCCGTTTGCCAAGTCTGACACGAAGATCTTGGATGATATTTACACTACTCATGTCCATGCTGATGATTCCTTCGACGACGATTCTCTTTTCATCATTGTTGAGAGTATCCTCAAACGTGCAACCCAAACTGTTGATAAAATTGTGCAGGGTACCCAAGTGCATGTGGAAGACATAGAGGAGCAAACTCCCAAAGCCAACTTCAGTGTACCATTGTGCACGCTTAAGGGAATCTCCTGCGAGATGCAATGCAAGCCTCCTGGTGAAGAAATCGCCCATAAGACAACACTGGCAATACTACACAAGCTGTCAAACTATTCATGGGAAGCAAAGGCATCACTGACCCTAGCAGCTTTTGCTATGGAGTTTGGTGAAATCTGGCTCCTTGCAGAGCTTCGTGAATCAAACCACCTTGCCAGGTCAATTGCAATTCTCAAACGACTCCCTGTTCTCCTCAAGGATTCTCAGCTGCACAAGCGCAGGCAAGCAATACTTGAGCTTAACAATCTGATCAAGGCCACACTGGAAGTCATTACATGCATCGACCAGTTCAACAAGCTTTCCAATTATGACCTGAAAGATGTCCCCGAGTTGTCGGGAACATTGGATCATGTTCCGGCGGACGTATATTGGGCTATCATGACTGTTGTGGCTTGTGCCACTAAGATCACACTACTCACTAGTGATGAGGACAAAGAATTTGACCTATCTCCCTATTCTCAAAAGATCCACTTTGTCCTTAACAATCTCAAGACACAGCTAACAATTTGCAGAGCACGAATTGAGGTCATAGAAAATTATAAAAAACTCAAGAAACTGTTCCGAACACCTACTGAAATTATGGAAATCGTTAAGGGTTTGTTATTTACAAAGGATAATGTCCAGCCACTCGTTGATTGTTCCACTAAACAAACGGCGAGCATCGAAATCCTAAGGAGGAAGAATGTGCTATTCTTCATTTCCAGCCTCGACATTACGGATGATGACATTTCGATTCTGAAACCGGTCCACGAGTTCACAAAGAAAGACAATCAGTATAAGATTGTTTGGATACCAATTGTGGAGCAATGGACCGATGAGCTGAGAAAGAAGTCTGAAATCCTGAAGAACAAGATGCCGTGGTATACGGTGCAGTACTCTGGACCTATTGCAGGCATCAAGTTCATCAAGGAGGAGTGGAACTTCAAGGGTAAGCCATTACTGGTAGTGATGAACCCACAAGGAAAGGTCGAACACTCTAACGCTCTTCACATGATTCGGGTGTGGGGAGTCAAGGCCTTCCCTTTCACAGAGACAACAGAGAAAGAGCTGTCATCAGATTCTCATGGAGGAATTTACTCTATAGTTGACGGAATTCACCCATCTGTGCCATCTTACATCAGAGAAAATAAGTACATCTTCTTCTATGGCGGTAAGGACAACGAATGGATTCAGCAATTCACAAAGAAAGCAACTGCCCTTGCAAACGATCCCATCTTGAAGGAAGCAAAGATTTACATAGAGTTGGTTTGCGTTGGAAAAGACAGCGAAGGAGAAGATGACCATGGCATTCTAGGCCGCTTCTGGACCGGTATAGAAAGCTTGTTCCTCACCAAAGTCCACAAGCACTTTGATCCGATCGGTCAAGAAATTCAGAAGCTTGTCTCTTACAAGAATGAAAGTGGATGGGTGTTGGTTACCAAAGGATCAACTTTGTTGGTCACAGGTCATGGTATCTCCGCATTGAAGGTGGTAGAGGACTTTGAGAAATGGAGGGAGCATGTCAAGGAGAAAGGGTTTGAGATTTGTTTCAAGGAATATTATGAGAACGTTATTCAGGATGGTCGACCTTGTTTCCGTCTTGACATTCCGGTGTCTACCGGAAAGGTTCCGGAGTCGATGAAGTGCCCTGATTGTCACCGTAGTATGGAGACTTTTATCAGTTACAAGTGCTGCCACATTGATGGTCCAACTGCACATCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SEO_C SEO_N

Protein Analysis

671

Amino Acids

76.49

Weight (kDa)

6.31

Isoelectric Point (pI)

37.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SEO_N PF14576 22 - 293 2.9e-83 Sieve element occlusion N-terminus
SEO_C PF14577 453 - 529 1.7e-13 Sieve element occlusion C-terminus
SEO_C PF14577 537 - 662 1.4e-30 Sieve element occlusion C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000511)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g02370 FvH4_4g02370 FvH4_4g02370 FvH4_4g02430 FvH4_4g02430 FvH4_4g02460 FvH4_4g02460 FvH4_4g02460 FvH4_4g02480 FvH4_4g02480 FvH4_4g02480 FvH4_4g02480 FvH4_6g05920
malus_domestica MD00G1111000.v1.1 MD08G1225800.v1.1 MD13G1205700.v1.1 MD13G1205800.v1.1 MD13G1205900.v1.1 MD13G1206000.v1.1 MD16G1207500.v1.1 MD16G1207600.v1.1
prunus_persica Prupe.1G028400_v2.0.a1 Prupe.1G028500_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028900_v2.0.a1 Prupe.1G029000_v2.0.a1 Prupe.1G029100_v2.0.a1
pyrus_communis pycom08g19680 pycom08g19730 pycom16g17460 pycom16g17500
rosa_chinensis RchiOBHm_Chr4g0389751 RchiOBHm_Chr4g0389771 RchiOBHm_Chr4g0389791 RchiOBHm_Chr4g0389861 RchiOBHm_Chr4g0389881 RchiOBHm_Chr4g0389901 RchiOBHm_Chr4g0412411
rosa_laevigata RLG00000008288 RLG00000009901 RLG00000009945 RLG00000009954 RLG00000009956 RLG00000009957 RLG00000009958 RLG00000019246
rosa_multiflora Rmu_co8275125.1_g000001 Rmu_sc0002083.1_g000003 Rmu_sc0002083.1_g000025 Rmu_sc0002604.1_g000016 Rmu_sc0004336.1_g000031 Rmu_sc0010423.1_g000003
rosa_roxburghii Rroxscaffold_5G00335790 Rroxscaffold_5G00335850 Rroxscaffold_5G00335970 Rroxscaffold_5G00335990
rosa_rugosa Rorug03G0325100 Rorug03G0325300 Rorug03G0325500.1 Rorug03G0326800 Rorug03G0327000 Rorug04G0104400
rosa_samantha Rh4AG029900 Rh4AG030000 Rh4AG030100 Rh4AG030200 Rh4AG030600 Rh4AG172900 Rh4BG023200 Rh4BG023900 Rh4BG024000 Rh4BG024100 Rh4BG172200 Rh4DG023300 Rh4DG023800 Rh4DG023900 Rh4DG024000 Rh4DG151600
rosa_wichuraiana Rw1G002330 Rw1G002340 Rw4G002260 Rw4G002270 Rw4G002280 Rw4G002300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 849
Acc65I GGTACC 1 cut(s) 200
AccB1I GGYRCC 1 cut(s) 200
AccI GTMKAC 3 cut(s) 1172, 1873, 1904
AccIII TCCGGA 1 cut(s) 1918
AciI CCGC 4 cut(s) 668, 1458, 1606, 1767
AclI AACGTT 1 cut(s) 1857
AclWI GGATC 5 cut(s) 663, 769, 1511, 1655, 1741
AcoI YGGCCR 1 cut(s) 7
AcsI RAATTY 4 cut(s) 746, 1380, 1401, 1677
AcuI CTGAAG 2 cut(s) 235, 1172
AfaI GTAC 4 cut(s) 202, 258, 1184, 1442
AfiI CCNNNNNNNGG 2 cut(s) 1199, 1954
AflII CTTAAG 1 cut(s) 272
AgeI ACCGGT 2 cut(s) 1051, 1616
AgsI TTSAA 5 cut(s) 601, 1234, 1528, 1774, 1840
AhlI ACTAGT 1 cut(s) 728
AjnI CCWGG 3 cut(s) 4, 307, 464
AjuI GAANNNNNNNTTGG 2 cut(s) 233, 265
Alw21I GWGCWC 2 cut(s) 269, 826
Alw26I GTCTC 3 cut(s) 1334, 1696, 1952
Alw44I GTGCAC 1 cut(s) 265
AlwI GGATC 5 cut(s) 663, 769, 1511, 1655, 1741
Ama87I CYCGRG 1 cut(s) 635
Aor13HI TCCGGA 1 cut(s) 1918
AoxI GGCC 5 cut(s) 7, 24, 564, 1323, 1603
ApaLI GTGCAC 1 cut(s) 265
ApeKI GCWGC 3 cut(s) 398, 517, 1980
ApoI RAATTY 4 cut(s) 746, 1380, 1401, 1677
ArsI GACNNNNNNTTYG 2 cut(s) 731, 763
AsiGI ACCGGT 2 cut(s) 1051, 1616
Asp700I GAANNNNTTC 2 cut(s) 866, 1476
Asp718I GGTACC 1 cut(s) 200
AspLEI GCGC 1 cut(s) 528
AspS9I GGNCC 5 cut(s) 1054, 1119, 1190, 1614, 1994
AsuHPI GGTGA 5 cut(s) 323, 431, 1397, 1630, 1937
AvaI CYCGRG 1 cut(s) 635
AvaII GGWCC 5 cut(s) 1054, 1119, 1190, 1614, 1994
AxyI CCTNAGG 1 cut(s) 980
BaeGI GKGCMC 2 cut(s) 269, 1937
BaeI ACNNNNGTAYC 2 cut(s) 192, 225
BalI TGGCCA 1 cut(s) 9
BanI GGYRCC 1 cut(s) 200
BarI GAAGNNNNNNTAC 2 cut(s) 1433, 1465
BauI CACGAG 1 cut(s) 1058
BbsI GAAGAC 1 cut(s) 225
Bbv12I GWGCWC 2 cut(s) 269, 826
BbvI GCAGC 3 cut(s) 410, 504, 1967
BccI CCATC 6 cut(s) 1417, 1426, 1529, 1707, 1862, 1985
BceAI ACGGC 2 cut(s) 978, 1150
BciT130I CCWGG 3 cut(s) 6, 309, 466
BciVI GTATCC 2 cut(s) 167, 1092
BclI TGATCA 1 cut(s) 558
BcoDI GTCTC 3 cut(s) 1334, 1696, 1952
BcuI ACTAGT 1 cut(s) 728
BfaI CTAG 3 cut(s) 395, 729, 1601
BfmI CTRYAG 1 cut(s) 1389
BfrI CTTAAG 1 cut(s) 272
BfuI GTATCC 2 cut(s) 167, 1092
BglII AGATCT 1 cut(s) 75
BisI GCNGC 4 cut(s) 399, 518, 1606, 1981
BlsI GCNGC 4 cut(s) 400, 519, 1607, 1982
BmcAI AGTACT 1 cut(s) 1184
Bme1390I CCNGG 3 cut(s) 6, 309, 466
Bme18I GGWCC 5 cut(s) 1054, 1119, 1190, 1614, 1994
BmeT110I CYCGRG 1 cut(s) 635
BmgT120I GGNCC 5 cut(s) 1054, 1119, 1190, 1614, 1994
BmiI GGNNCC 3 cut(s) 202, 431, 1917
BmrFI CCNGG 3 cut(s) 6, 309, 466
BmsI GCATC 6 cut(s) 282, 392, 597, 978, 1152, 1212
BpiI GAAGAC 1 cut(s) 225
BpuEI CTTGAG 4 cut(s) 488, 563, 782, 842
BsaJI CCNNGG 2 cut(s) 1166, 1591
BsaWI WCCGGW 5 cut(s) 1051, 1616, 1897, 1907, 1918
BsaXI ACNNNNNCTCC 2 cut(s) 1808, 1838
Bsc4I CCNNNNNNNGG 2 cut(s) 1199, 1954
Bse118I RCCGGY 2 cut(s) 1051, 1616
Bse1I ACTGG 4 cut(s) 342, 576, 595, 1254
Bse21I CCTNAGG 1 cut(s) 980
Bse3DI GCAATG 2 cut(s) 302, 1121
BseAI TCCGGA 1 cut(s) 1918
BseBI CCWGG 3 cut(s) 6, 309, 466
BseDI CCNNGG 2 cut(s) 1166, 1591
BseGI GGATG 4 cut(s) 88, 1033, 1718, 1873
BseLI CCNNNNNNNGG 2 cut(s) 1199, 1954
BseMI GCAATG 2 cut(s) 302, 1121
BseMII CTCAG 3 cut(s) 42, 527, 1121
BseNI ACTGG 4 cut(s) 342, 576, 595, 1254
BseRI GAGGAG 3 cut(s) 242, 491, 1235
BseSI GKGCMC 2 cut(s) 269, 1937
BseXI GCAGC 3 cut(s) 410, 504, 1967
BsgI GTGCAG 4 cut(s) 215, 503, 1199, 1986
Bsh1285I CGRYCG 1 cut(s) 1668
BshFI GGCC 5 cut(s) 9, 26, 566, 1325, 1605
BshNI GGYRCC 1 cut(s) 200
BshTI ACCGGT 2 cut(s) 1051, 1616
BsiEI CGRYCG 1 cut(s) 1668
BsiHKAI GWGCWC 2 cut(s) 269, 826
BsiHKCI CYCGRG 1 cut(s) 635
BsiSI CCGG 6 cut(s) 665, 1052, 1617, 1898, 1908, 1919
BslFI GGGAC 1 cut(s) 617
BslI CCNNNNNNNGG 2 cut(s) 1199, 1954
BsmAI GTCTC 3 cut(s) 1334, 1696, 1952
BsmFI GGGAC 1 cut(s) 617
BsmI GAATGC 1 cut(s) 1596
BsnI GGCC 5 cut(s) 9, 26, 566, 1325, 1605
BsoBI CYCGRG 1 cut(s) 635
Bsp1286I GDGCHC 3 cut(s) 269, 826, 1937
Bsp13I TCCGGA 1 cut(s) 1918
Bsp143I GATC 9 cut(s) 75, 558, 655, 714, 774, 1516, 1660, 1665, 1733
Bsp19I CCATGG 1 cut(s) 1591
BspACI CCGC 4 cut(s) 668, 1458, 1606, 1767
BspANI GGCC 5 cut(s) 9, 26, 566, 1325, 1605
BspCNI CTCAG 3 cut(s) 41, 526, 1122
BspEI TCCGGA 1 cut(s) 1918
BspHI TCATGA 1 cut(s) 687
BspLI GGNNCC 3 cut(s) 202, 431, 1917
BspPI GGATC 5 cut(s) 663, 769, 1511, 1655, 1741
BspQI GCTCTTC 1 cut(s) 1299
BspT107I GGYRCC 1 cut(s) 200
BspTI CTTAAG 1 cut(s) 272
BsrDI GCAATG 2 cut(s) 302, 1121
BsrFI RCCGGY 2 cut(s) 1051, 1616
BsrI ACTGG 4 cut(s) 342, 576, 595, 1254
BssAI RCCGGY 2 cut(s) 1051, 1616
BssECI CCNNGG 2 cut(s) 1166, 1591
BssMI GATC 9 cut(s) 75, 558, 655, 714, 774, 1516, 1660, 1665, 1733
BssNAI GTATAC 1 cut(s) 1173
BssSI CACGAG 1 cut(s) 1058
BssT1I CCWWGG 1 cut(s) 1591
Bst1107I GTATAC 1 cut(s) 1173
Bst2BI CACGAG 1 cut(s) 1058
Bst2UI CCWGG 3 cut(s) 6, 309, 466
Bst4CI ACNGT 5 cut(s) 181, 694, 867, 1177, 1949
Bst6I CTCTTC 1 cut(s) 1299
BstAFI CTTAAG 1 cut(s) 272
BstAPI GCANNNNNTGC 1 cut(s) 404
BstC8I GCNNGC 6 cut(s) 269, 302, 530, 534, 967, 1201
BstDEI CTNAG 5 cut(s) 28, 513, 711, 980, 1130
BstDSI CCRYGG 2 cut(s) 1166, 1591
BstEII GGTNACC 1 cut(s) 1723
BstENI CCTNNNNNAGG 1 cut(s) 1197
BstF5I GGATG 4 cut(s) 88, 1033, 1718, 1873
BstHHI GCGC 1 cut(s) 528
BstKTI GATC 9 cut(s) 78, 561, 658, 717, 777, 1519, 1663, 1668, 1736
BstMAI GTCTC 3 cut(s) 1334, 1696, 1952
BstMBI GATC 9 cut(s) 75, 558, 655, 714, 774, 1516, 1660, 1665, 1733
BstMCI CGRYCG 1 cut(s) 1668
BstMWI GCNNNNNNNGC 4 cut(s) 301, 404, 523, 1602
BstNI CCWGG 3 cut(s) 6, 309, 466
BstNSI RCATGY 3 cut(s) 215, 588, 1811
BstPI GGTNACC 1 cut(s) 1723
BstSCI CCNGG 3 cut(s) 4, 307, 464
BstSFI CTRYAG 1 cut(s) 1389
BstSLI GKGCMC 2 cut(s) 269, 1937
BstV1I GCAGC 3 cut(s) 410, 504, 1967
BstV2I GAAGAC 1 cut(s) 225
BstX2I RGATCY 2 cut(s) 75, 774
BstXI CCANNNNNNTGG 1 cut(s) 1991
BstYI RGATCY 2 cut(s) 75, 774
BstZ17I GTATAC 1 cut(s) 1173
Bsu36I CCTNAGG 1 cut(s) 980
BsuI GTATCC 2 cut(s) 167, 1092
BsuRI GGCC 5 cut(s) 9, 26, 566, 1325, 1605
BtgI CCRYGG 2 cut(s) 1166, 1591
BtsCI GGATG 4 cut(s) 88, 1033, 1718, 1873
BtsIMutI CAGTG 4 cut(s) 259, 335, 386, 569
Cac8I GCNNGC 6 cut(s) 269, 302, 530, 534, 967, 1201
CciI TCATGA 1 cut(s) 687
CfoI GCGC 1 cut(s) 528
Cfr10I RCCGGY 2 cut(s) 1051, 1616
Cfr13I GGNCC 5 cut(s) 1054, 1119, 1190, 1614, 1994
Csp6I GTAC 4 cut(s) 201, 257, 1183, 1441
CspAI ACCGGT 2 cut(s) 1051, 1616
CviQI GTAC 4 cut(s) 201, 257, 1183, 1441
DdeI CTNAG 5 cut(s) 28, 513, 711, 980, 1130
DpnI GATC 9 cut(s) 77, 560, 657, 716, 776, 1518, 1662, 1667, 1735
DpnII GATC 9 cut(s) 75, 558, 655, 714, 774, 1516, 1660, 1665, 1733
EaeI YGGCCR 1 cut(s) 7
Eam1104I CTCTTC 1 cut(s) 1299
EarI CTCTTC 1 cut(s) 1299
EciI GGCGGA 1 cut(s) 683
Eco130I CCWWGG 1 cut(s) 1591
Eco147I AGGCCT 2 cut(s) 26, 1325
Eco47I GGWCC 5 cut(s) 1054, 1119, 1190, 1614, 1994
Eco57I CTGAAG 2 cut(s) 235, 1172
Eco81I CCTNAGG 1 cut(s) 980
Eco88I CYCGRG 1 cut(s) 635
Eco91I GGTNACC 1 cut(s) 1723
EcoNI CCTNNNNNAGG 1 cut(s) 1197
EcoO65I GGTNACC 1 cut(s) 1723
EcoRI GAATTC 1 cut(s) 1401
EcoRII CCWGG 3 cut(s) 4, 307, 464
EcoT14I CCWWGG 1 cut(s) 1591
EcoT22I ATGCAT 1 cut(s) 590
ErhI CCWWGG 1 cut(s) 1591
FalI AAGNNNNNCTT 6 cut(s) 525, 557, 764, 796, 1430, 1462
FaqI GGGAC 1 cut(s) 617
FbaI TGATCA 1 cut(s) 558
FblI GTMKAC 3 cut(s) 1172, 1873, 1904
Fnu4HI GCNGC 4 cut(s) 399, 518, 1606, 1981
FokI GGATG 4 cut(s) 95, 1040, 1725, 1880
Fsp4HI GCNGC 4 cut(s) 399, 518, 1606, 1981
FspBI CTAG 3 cut(s) 395, 729, 1601
GlaI GCGC 1 cut(s) 527
GluI GCNGC 4 cut(s) 399, 518, 1606, 1981
HaeIII GGCC 5 cut(s) 9, 26, 566, 1325, 1605
HapII CCGG 6 cut(s) 665, 1052, 1617, 1898, 1908, 1919
HhaI GCGC 1 cut(s) 528
Hin6I GCGC 1 cut(s) 526
HinP1I GCGC 1 cut(s) 526
HincII GTYRAC 2 cut(s) 1396, 1874
HindII GTYRAC 2 cut(s) 1396, 1874
HindIII AAGCTT 3 cut(s) 605, 1627, 1685
HpaII CCGG 6 cut(s) 665, 1052, 1617, 1898, 1908, 1919
HphI GGTGA 5 cut(s) 323, 431, 1397, 1630, 1937
Hpy166II GTNNAC 9 cut(s) 257, 267, 1057, 1065, 1173, 1396, 1648, 1874, 1905
Hpy188I TCNGA 9 cut(s) 67, 558, 872, 1047, 1144, 1366, 1430, 1665, 1683
Hpy8I GTNNAC 9 cut(s) 257, 267, 1057, 1065, 1173, 1396, 1648, 1874, 1905
Hpy99I CGWCG 2 cut(s) 128, 131
HpyAV CCTTC 5 cut(s) 130, 1336, 1522, 1571, 1768
HpyCH4III ACNGT 5 cut(s) 181, 694, 867, 1177, 1949
HpyCH4IV ACGT 3 cut(s) 166, 672, 1857
HpyF10VI GCNNNNNNNGC 4 cut(s) 301, 404, 523, 1602
HpyF3I CTNAG 5 cut(s) 28, 513, 711, 980, 1130
HpySE526I ACGT 3 cut(s) 166, 672, 1857
HspAI GCGC 1 cut(s) 526
Kpn2I TCCGGA 1 cut(s) 1918
KpnI GGTACC 1 cut(s) 204
Ksp22I TGATCA 1 cut(s) 558
Kzo9I GATC 9 cut(s) 75, 558, 655, 714, 774, 1516, 1660, 1665, 1733
LguI GCTCTTC 1 cut(s) 1299
LmnI GCTCC 4 cut(s) 229, 435, 1111, 1804
Lsp1109I GCAGC 3 cut(s) 410, 504, 1967
LweI GCATC 6 cut(s) 282, 392, 597, 978, 1152, 1212
MaeI CTAG 3 cut(s) 395, 729, 1601
MaeII ACGT 3 cut(s) 166, 672, 1857
MaeIII GTNAC 4 cut(s) 1723, 1747, 1943, 1970
MalI GATC 9 cut(s) 77, 560, 657, 716, 776, 1518, 1662, 1667, 1735
MboI GATC 9 cut(s) 75, 558, 655, 714, 774, 1516, 1660, 1665, 1733
MfeI CAATTG 2 cut(s) 471, 1104
MflI RGATCY 2 cut(s) 75, 774
MhlI GDGCHC 3 cut(s) 269, 826, 1937
MlsI TGGCCA 1 cut(s) 9
MluNI TGGCCA 1 cut(s) 9
MlyI GAGTC 3 cut(s) 483, 1326, 1931
MmeI TCCRAC 1 cut(s) 1543
Mox20I TGGCCA 1 cut(s) 9
Mph1103I ATGCAT 1 cut(s) 590
MroI TCCGGA 1 cut(s) 1918
MroXI GAANNNNTTC 2 cut(s) 866, 1476
MscI TGGCCA 1 cut(s) 9
MseI TTAA 4 cut(s) 273, 549, 789, 900
MslI CAYNNNNRTG 4 cut(s) 105, 111, 1898, 1989
Msp20I TGGCCA 1 cut(s) 9
MspA1I CMGCKG 1 cut(s) 517
MspCI CTTAAG 1 cut(s) 272
MspI CCGG 6 cut(s) 665, 1052, 1617, 1898, 1908, 1919
MspR9I CCNGG 3 cut(s) 6, 309, 466
MunI CAATTG 2 cut(s) 471, 1104
Mva1269I GAATGC 1 cut(s) 1596
MvaI CCWGG 3 cut(s) 6, 309, 466
MwoI GCNNNNNNNGC 4 cut(s) 301, 404, 523, 1602
NcoI CCATGG 1 cut(s) 1591
NdeII GATC 9 cut(s) 75, 558, 655, 714, 774, 1516, 1660, 1665, 1733
NlaIV GGNNCC 3 cut(s) 202, 431, 1917
NmuCI GTSAC 2 cut(s) 1747, 1943
NsiI ATGCAT 1 cut(s) 590
NspI RCATGY 3 cut(s) 215, 588, 1811
PagI TCATGA 1 cut(s) 687
PceI AGGCCT 2 cut(s) 26, 1325
PciSI GCTCTTC 1 cut(s) 1299
PctI GAATGC 1 cut(s) 1596
PdmI GAANNNNTTC 2 cut(s) 866, 1476
PfeI GAWTC 9 cut(s) 116, 131, 279, 449, 509, 1042, 1303, 1367, 1477
PinAI ACCGGT 2 cut(s) 1051, 1616
PkrI GCNGC 4 cut(s) 400, 519, 1607, 1982
Ple19I CGATCG 1 cut(s) 1668
PleI GAGTC 3 cut(s) 483, 1325, 1930
PpsI GAGTC 3 cut(s) 483, 1325, 1930
PsiI TTATAA 1 cut(s) 849
Psp1406I AACGTT 1 cut(s) 1857
Psp6I CCWGG 3 cut(s) 4, 307, 464
PspEI GGTNACC 1 cut(s) 1723
PspGI CCWGG 3 cut(s) 4, 307, 464
PspN4I GGNNCC 3 cut(s) 202, 431, 1917
PspPI GGNCC 5 cut(s) 1054, 1119, 1190, 1614, 1994
PsuI RGATCY 2 cut(s) 75, 774
PvuI CGATCG 1 cut(s) 1668
PvuII CAGCTG 1 cut(s) 517
RsaI GTAC 4 cut(s) 202, 258, 1184, 1442
RsaNI GTAC 4 cut(s) 201, 257, 1183, 1441
RseI CAYNNNNRTG 4 cut(s) 105, 111, 1898, 1989
SalI GTCGAC 1 cut(s) 1872
SapI GCTCTTC 1 cut(s) 1299
SaqAI TTAA 4 cut(s) 273, 549, 789, 900
SatI GCNGC 4 cut(s) 399, 518, 1606, 1981
Sau3AI GATC 9 cut(s) 75, 558, 655, 714, 774, 1516, 1660, 1665, 1733
Sau96I GGNCC 5 cut(s) 1054, 1119, 1190, 1614, 1994
ScaI AGTACT 1 cut(s) 1184
SchI GAGTC 3 cut(s) 483, 1326, 1931
ScrFI CCNGG 3 cut(s) 6, 309, 466
SduI GDGCHC 3 cut(s) 269, 826, 1937
SfaNI GCATC 6 cut(s) 282, 392, 597, 978, 1152, 1212
SfcI CTRYAG 1 cut(s) 1389
SinI GGWCC 5 cut(s) 1054, 1119, 1190, 1614, 1994
SmiMI CAYNNNNRTG 4 cut(s) 105, 111, 1898, 1989
SmlI CTYRAG 5 cut(s) 272, 503, 542, 797, 857
SmoI CTYRAG 5 cut(s) 272, 503, 542, 797, 857
SpeI ACTAGT 1 cut(s) 728
SseBI AGGCCT 2 cut(s) 26, 1325
SsiI CCGC 4 cut(s) 668, 1458, 1606, 1767
SspI AATATT 1 cut(s) 1847
SspMI CTAG 3 cut(s) 395, 729, 1601
StuI AGGCCT 2 cut(s) 26, 1325
StyD4I CCNGG 3 cut(s) 4, 307, 464
StyI CCWWGG 1 cut(s) 1591
TaaI ACNGT 5 cut(s) 181, 694, 867, 1177, 1949
TaiI ACGT 3 cut(s) 169, 675, 1860
TaqI TCGA 8 cut(s) 123, 591, 972, 1017, 1040, 1278, 1873, 1925
TaqII GACCGA 2 cut(s) 1136, 1658
TatI WGTACW 2 cut(s) 1182, 1440
TauI GCSGC 1 cut(s) 1608
TfiI GAWTC 9 cut(s) 116, 131, 279, 449, 509, 1042, 1303, 1367, 1477
Tru1I TTAA 4 cut(s) 273, 549, 789, 900
Tru9I TTAA 4 cut(s) 273, 549, 789, 900
TscAI CASTG 4 cut(s) 259, 342, 393, 576
TseFI GTSAC 2 cut(s) 1747, 1943
TseI GCWGC 3 cut(s) 398, 517, 1980
Tsp45I GTSAC 2 cut(s) 1747, 1943
TspDTI ATGAA 7 cut(s) 130, 357, 994, 1201, 1274, 1719, 1943
TspGWI ACGGA 3 cut(s) 1040, 1413, 1874
TspRI CASTG 4 cut(s) 259, 342, 393, 576
Vha464I CTTAAG 1 cut(s) 272
VneI GTGCAC 1 cut(s) 265
VpaK11BI GGWCC 5 cut(s) 1054, 1119, 1190, 1614, 1994
XagI CCTNNNNNAGG 1 cut(s) 1197
XapI RAATTY 4 cut(s) 746, 1380, 1401, 1677
XceI RCATGY 3 cut(s) 215, 588, 1811
XmiI GTMKAC 3 cut(s) 1172, 1873, 1904
XmnI GAANNNNTTC 2 cut(s) 866, 1476
XspI CTAG 3 cut(s) 395, 729, 1601
ZrmI AGTACT 1 cut(s) 1184
Zsp2I ATGCAT 1 cut(s) 590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.