Rh4DG023800

Sieve element occlusion

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000511
Physical Location & Seq
Forward (+)
3898009 .. 3901383
3375 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG023800.1

Sequence Viewer

Length: 1938 bp
ATGTCTGACAAGAAGATCTTGGAAGAGATTTACACAACTCATGTCCATACTGATGAATCCTTCGATGACGATTCTCTCTTCGTCATTGTTGAGAACATCCTCAAGCGTGCAATCCAAAATGTTGATAAAATAGTACAGGGTACTCAAGTGCATGTGGACAACATAGAGGAGAAAACTCCCAAAGCCAGTTTCAGCGTACCATTGTGCACACTAAAGGGAATCTCCTGCGAGATGCAATGCAAGCCTCCTGGTGACGAAATCGCTCACAACACAACAATGGCAATACTCGAGAAGCTCTCAAAGTATTCATGGGAAGCAAAGGCATCACTGACTCTAGCAGCTTTTGCTATGGAATATGGTGAATTCTGGCTCCTGGCGCAGCTTCGCGAATCAAACCACCTTGCCAAATCAATTGCAATCCTCAAACGAGTCCCAGTTCTCCTCAAGCCGTCTGAGCTACACAAGCGCAGGCAATCAATTCTTGAGCTTAACAATCTGATCAAGGCCACATTGCAAGTGATCGAATGCATTGACCAGTTCAATAAGTATTCCACTTATGACCCAAAAGATGTCCCCGATTTGTCGATAGCATTGGATCATATTCCGGTTGATGTCTATTGGCTTATCATCACTGTTGTTGCTTGTGCTACTAAGATCACTATACTCACTAGTGATGAGGACAAGGAGTATGATCTAGCCCCCTATTCTCAAAAAATCCACTATATCCTTAACAAGCTAACGATTTTGCTGAGAGAGGAGGCAGAGACTCACAGAAGGATCATGAAATTGTTCCGAACCCCTACTGAAATTATGGAGGTTTTCAAGGGATTGATATTTACAAAGGATAATGTCCAGCCACTCATCGATGGTTCAACTAAGCAAACGGTTAATATCGACATCCTAAGGAGAAAGAATGTGTTATTGTTCATTTCCACCCTAGACGTCTCGGACGATGACATTTCGATTCTGAAACCGATCCACGAGTTCACAAAGAGAGACAATCAGTATAAGATTGTCTGGATTCCAATTGTGGAGCAATGGACTGATGACCTGAGAAAAAAGTTTGATATCCTGAAGAACAAGATGCCGTGGTTTACAGTGCGGTACTCTGGACCTATTGCAGGCATCAAGTTCATCAAGGAGGAGTGGAATTTCAAGGGTAAGCCTATAGTGGTGGTGATGAACCCACAAGGAAAGGTAGAACACTCAAACGCTCTCCACATGATTCGAGTATGGGGAGCCAAGGCCTTCCCTTTCACAAAGACAACAGAAGAAGAACTGTCACATTCTCATGCAGGAAAATGGGTTGGCTCCGTTGTTGAAGGAACTCACCCATCTGTGCACACTTGGATCAAAGAGGATAAGTACATATTCTTCTATGGCGGTAAAGACAACGAATGGATTCAGCAATTCACAAAGAAAGCGACTGCCCTTGCAAATGATCCAATCTTCAAGGAAGCAAAGATTCACCTAGAGTTGGTCTGCGTCGGAAAAGGCAGCAGAGGAGAAGATGACCATGAGGTTCTAGGCCGCTTCTGGACCAGTGTAGAGAGCCTGTTCTTCACCAAAGGCCACAAGCAGATGGACTCTGACAATCAAGAAATCCAGAAGCTTCTCTCTTACAAAAACGAAAGTGGATGGGCGGTGCTTAGCAAAGGGTCAACTGTGTTGGTCACAGGTCATGGGGTCTCGATGTTGAAGGTGGTTGAGGACTTTGAGAAATGGAAGGACCATGTCAAGGAGAAAGGGTTTGAATTTTGTTTCAAGGCCTATCATGAGAAGATTAGTCAGGCTAGTCGACCTTGCTGCCGTCTGGACATTCCGGGGTCTAATGGAAAGGTTCCGGAGTCGATGAGGTGCCCTGATTGCCACCATAATATGGAGACTTTTATCAGTTACAAGTGCTGCCACATTGATGGTCCTAATGCACGTCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SEO_C SEO_N

Protein Analysis

645

Amino Acids

74.06

Weight (kDa)

6.82

Isoelectric Point (pI)

38.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SEO_N PF14576 2 - 251 4.7e-78 Sieve element occlusion N-terminus
SEO_C PF14577 439 - 504 2.3e-14 Sieve element occlusion C-terminus
SEO_C PF14577 511 - 637 1.7e-33 Sieve element occlusion C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000511)

Species Orthologous Gene IDs
fragaria_vesca FvH4_4g02370 FvH4_4g02370 FvH4_4g02370 FvH4_4g02430 FvH4_4g02430 FvH4_4g02460 FvH4_4g02460 FvH4_4g02460 FvH4_4g02480 FvH4_4g02480 FvH4_4g02480 FvH4_4g02480 FvH4_6g05920
malus_domestica MD00G1111000.v1.1 MD08G1225800.v1.1 MD13G1205700.v1.1 MD13G1205800.v1.1 MD13G1205900.v1.1 MD13G1206000.v1.1 MD16G1207500.v1.1 MD16G1207600.v1.1
prunus_persica Prupe.1G028400_v2.0.a1 Prupe.1G028500_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028800_v2.0.a1 Prupe.1G028900_v2.0.a1 Prupe.1G029000_v2.0.a1 Prupe.1G029100_v2.0.a1
pyrus_communis pycom08g19680 pycom08g19730 pycom16g17460 pycom16g17500
rosa_chinensis RchiOBHm_Chr4g0389751 RchiOBHm_Chr4g0389771 RchiOBHm_Chr4g0389791 RchiOBHm_Chr4g0389861 RchiOBHm_Chr4g0389881 RchiOBHm_Chr4g0389901 RchiOBHm_Chr4g0412411
rosa_laevigata RLG00000008288 RLG00000009901 RLG00000009945 RLG00000009954 RLG00000009956 RLG00000009957 RLG00000009958 RLG00000019246
rosa_multiflora Rmu_co8275125.1_g000001 Rmu_sc0002083.1_g000003 Rmu_sc0002083.1_g000025 Rmu_sc0002604.1_g000016 Rmu_sc0004336.1_g000031 Rmu_sc0010423.1_g000003
rosa_roxburghii Rroxscaffold_5G00335790 Rroxscaffold_5G00335850 Rroxscaffold_5G00335970 Rroxscaffold_5G00335990
rosa_rugosa Rorug03G0325100 Rorug03G0325300 Rorug03G0325500.1 Rorug03G0326800 Rorug03G0327000 Rorug04G0104400
rosa_samantha Rh4AG029900 Rh4AG030000 Rh4AG030100 Rh4AG030200 Rh4AG030600 Rh4AG172900 Rh4BG023200 Rh4BG023900 Rh4BG024000 Rh4BG024100 Rh4BG172200 Rh4DG023300 Rh4DG023800 Rh4DG023900 Rh4DG024000 Rh4DG151600
rosa_wichuraiana Rw1G002330 Rw1G002340 Rw4G002260 Rw4G002270 Rw4G002280 Rw4G002300

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 945
AccB1I GGYRCC 1 cut(s) 1857
AccB7I CCANNNNNTGG 1 cut(s) 1879
AccI GTMKAC 1 cut(s) 1798
AccII CGCG 1 cut(s) 387
AccIII TCCGGA 1 cut(s) 1843
AciI CCGC 4 cut(s) 1102, 1383, 1531, 1643
AclWI GGATC 5 cut(s) 603, 785, 970, 1358, 1436
AcsI RAATTY 3 cut(s) 362, 1150, 1754
AcuI CTGAAG 1 cut(s) 1094
AcyI GRCGYC 1 cut(s) 942
AfaI GTAC 5 cut(s) 135, 142, 198, 1106, 1367
AfiI CCNNNNNNNGG 4 cut(s) 1121, 1477, 1738, 1879
AgsI TTSAA 9 cut(s) 541, 823, 873, 1156, 1322, 1453, 1699, 1754, 1765
AhlI ACTAGT 1 cut(s) 668
AjiI CACGTC 1 cut(s) 1931
AjnI CCWGG 2 cut(s) 247, 372
AjuI GAANNNNNNNTTGG 2 cut(s) 173, 205
AluBI AGCT 7 cut(s) 295, 341, 382, 457, 487, 736, 1612
AluI AGCT 7 cut(s) 295, 341, 382, 457, 487, 736, 1612
Alw21I GWGCWC 2 cut(s) 209, 1344
Alw26I GTCTC 5 cut(s) 758, 949, 990, 1693, 1877
Alw44I GTGCAC 2 cut(s) 205, 1340
AlwI GGATC 5 cut(s) 603, 785, 970, 1358, 1436
Ama87I CYCGRG 1 cut(s) 287
Aor13HI TCCGGA 1 cut(s) 1843
AoxI GGCC 5 cut(s) 504, 1245, 1528, 1570, 1767
ApaLI GTGCAC 2 cut(s) 205, 1340
ApeKI GCWGC 5 cut(s) 338, 379, 1497, 1806, 1905
ApoI RAATTY 3 cut(s) 362, 1150, 1754
Asp700I GAANNNNTTC 2 cut(s) 788, 1401
AspLEI GCGC 2 cut(s) 379, 468
AspS9I GGNCC 4 cut(s) 1112, 1539, 1729, 1919
AsuC2I CCSGG 1 cut(s) 1824
AsuHPI GGTGA 6 cut(s) 263, 371, 1189, 1322, 1460, 1555
AvaI CYCGRG 1 cut(s) 287
AvaII GGWCC 4 cut(s) 1112, 1539, 1729, 1919
AxyI CCTNAGG 1 cut(s) 902
BaeGI GKGCMC 3 cut(s) 209, 1344, 1862
BaeI ACNNNNGTAYC 2 cut(s) 132, 165
BanI GGYRCC 1 cut(s) 1857
BarI GAAGNNNNNNTAC 2 cut(s) 1358, 1390
BauI CACGAG 1 cut(s) 980
Bbv12I GWGCWC 2 cut(s) 209, 1344
BbvI GCAGC 5 cut(s) 350, 391, 1509, 1793, 1892
BccI CCATC 5 cut(s) 860, 1342, 1576, 1632, 1910
BceAI ACGGC 3 cut(s) 433, 1072, 1794
BcgI CGANNNNNNTGC 2 cut(s) 1788, 1822
BciT130I CCWGG 2 cut(s) 249, 374
BclI TGATCA 1 cut(s) 498
BcnI CCSGG 1 cut(s) 1824
BcoDI GTCTC 5 cut(s) 758, 949, 990, 1693, 1877
BcuI ACTAGT 1 cut(s) 668
BfaI CTAG 7 cut(s) 335, 669, 695, 938, 1472, 1526, 1794
BfmI CTRYAG 1 cut(s) 1167
BglII AGATCT 1 cut(s) 15
BisI GCNGC 6 cut(s) 339, 380, 1498, 1531, 1807, 1906
BlpI GCTNAGC 1 cut(s) 1649
BlsI GCNGC 6 cut(s) 340, 381, 1499, 1532, 1808, 1907
Bme1390I CCNGG 3 cut(s) 249, 374, 1824
Bme18I GGWCC 4 cut(s) 1112, 1539, 1729, 1919
BmeT110I CYCGRG 1 cut(s) 287
BmgBI CACGTC 1 cut(s) 1931
BmgT120I GGNCC 4 cut(s) 1112, 1539, 1729, 1919
BmiI GGNNCC 5 cut(s) 371, 1240, 1312, 1842, 1859
BmrFI CCNGG 3 cut(s) 249, 374, 1824
BmrI ACTGGG 1 cut(s) 428
BmsI GCATC 4 cut(s) 222, 332, 1074, 1134
BmuI ACTGGG 1 cut(s) 428
BplI GAGNNNNNCTC 2 cut(s) 281, 313
Bpu1102I GCTNAGC 1 cut(s) 1649
BpuEI CTTGAG 4 cut(s) 86, 129, 428, 503
BpuMI CCSGG 1 cut(s) 1824
Bsa29I ATCGAT 1 cut(s) 864
BsaHI GRCGYC 1 cut(s) 942
BsaI GGTCTC 1 cut(s) 1693
BsaJI CCNNGG 3 cut(s) 1088, 1242, 1823
BsaWI WCCGGW 2 cut(s) 604, 1843
BsaXI ACNNNNNCTCC 2 cut(s) 1733, 1763
Bsc4I CCNNNNNNNGG 4 cut(s) 1121, 1477, 1738, 1879
Bse1I ACTGG 4 cut(s) 186, 434, 535, 1542
Bse21I CCTNAGG 1 cut(s) 902
Bse3DI GCAATG 3 cut(s) 242, 509, 1043
BseAI TCCGGA 1 cut(s) 1843
BseBI CCWGG 2 cut(s) 249, 374
BseCI ATCGAT 1 cut(s) 864
BseDI CCNNGG 3 cut(s) 1088, 1242, 1823
BseGI GGATG 3 cut(s) 96, 897, 1643
BseLI CCNNNNNNNGG 4 cut(s) 1121, 1477, 1738, 1879
BseMI GCAATG 3 cut(s) 242, 509, 1043
BseMII CTCAG 3 cut(s) 444, 740, 1043
BseNI ACTGG 4 cut(s) 186, 434, 535, 1542
BseRI GAGGAG 5 cut(s) 182, 431, 770, 1157, 1518
BseSI GKGCMC 3 cut(s) 209, 1344, 1862
BseXI GCAGC 5 cut(s) 350, 391, 1509, 1793, 1892
Bsh1236I CGCG 1 cut(s) 387
BshFI GGCC 5 cut(s) 506, 1247, 1530, 1572, 1769
BshNI GGYRCC 1 cut(s) 1857
BshVI ATCGAT 1 cut(s) 864
BsiHKAI GWGCWC 2 cut(s) 209, 1344
BsiHKCI CYCGRG 1 cut(s) 287
BsiSI CCGG 3 cut(s) 605, 1823, 1844
BslFI GGGAC 2 cut(s) 416, 557
BslI CCNNNNNNNGG 4 cut(s) 1121, 1477, 1738, 1879
BsmAI GTCTC 5 cut(s) 758, 949, 990, 1693, 1877
BsmBI CGTCTC 1 cut(s) 949
BsmFI GGGAC 2 cut(s) 416, 557
BsmI GAATGC 1 cut(s) 530
BsnI GGCC 5 cut(s) 506, 1247, 1530, 1572, 1769
Bso31I GGTCTC 1 cut(s) 1693
BsoBI CYCGRG 1 cut(s) 287
Bsp1286I GDGCHC 3 cut(s) 209, 1344, 1862
Bsp13I TCCGGA 1 cut(s) 1843
Bsp1720I GCTNAGC 1 cut(s) 1649
Bsp68I TCGCGA 1 cut(s) 387
BspACI CCGC 4 cut(s) 1102, 1383, 1531, 1643
BspANI GGCC 5 cut(s) 506, 1247, 1530, 1572, 1769
BspCNI CTCAG 3 cut(s) 445, 741, 1044
BspDI ATCGAT 1 cut(s) 864
BspEI TCCGGA 1 cut(s) 1843
BspFNI CGCG 1 cut(s) 387
BspHI TCATGA 2 cut(s) 780, 1774
BspLI GGNNCC 5 cut(s) 371, 1240, 1312, 1842, 1859
BspPI GGATC 5 cut(s) 603, 785, 970, 1358, 1436
BspT107I GGYRCC 1 cut(s) 1857
BspTNI GGTCTC 1 cut(s) 1693
BsrDI GCAATG 3 cut(s) 242, 509, 1043
BsrI ACTGG 4 cut(s) 186, 434, 535, 1542
BssECI CCNNGG 3 cut(s) 1088, 1242, 1823
BssNI GRCGYC 1 cut(s) 942
BssSI CACGAG 1 cut(s) 980
BssT1I CCWWGG 1 cut(s) 1242
Bst2BI CACGAG 1 cut(s) 980
Bst2UI CCWGG 2 cut(s) 249, 374
Bst4CI ACNGT 5 cut(s) 634, 886, 1099, 1281, 1666
Bst6I CTCTTC 2 cut(s) 18, 83
BstACI GRCGYC 1 cut(s) 942
BstAPI GCANNNNNTGC 1 cut(s) 344
BstC8I GCNNGC 4 cut(s) 108, 242, 470, 1123
BstDEI CTNAG 7 cut(s) 453, 651, 749, 876, 902, 1052, 1649
BstDSI CCRYGG 1 cut(s) 1088
BstENI CCTNNNNNAGG 1 cut(s) 1119
BstF5I GGATG 3 cut(s) 96, 897, 1643
BstFNI CGCG 1 cut(s) 387
BstHHI GCGC 2 cut(s) 379, 468
BstMAI GTCTC 5 cut(s) 758, 949, 990, 1693, 1877
BstMWI GCNNNNNNNGC 6 cut(s) 241, 344, 376, 454, 463, 1866
BstNI CCWGG 2 cut(s) 249, 374
BstNSI RCATGY 1 cut(s) 155
BstSCI CCNGG 3 cut(s) 247, 372, 1822
BstSFI CTRYAG 1 cut(s) 1167
BstSLI GKGCMC 3 cut(s) 209, 1344, 1862
BstUI CGCG 1 cut(s) 387
BstV1I GCAGC 5 cut(s) 350, 391, 1509, 1793, 1892
BstX2I RGATCY 1 cut(s) 15
BstXI CCANNNNNNTGG 1 cut(s) 1916
BstYI RGATCY 1 cut(s) 15
Bsu15I ATCGAT 1 cut(s) 864
Bsu36I CCTNAGG 1 cut(s) 902
BsuRI GGCC 5 cut(s) 506, 1247, 1530, 1572, 1769
BsuTUI ATCGAT 1 cut(s) 864
BtgI CCRYGG 1 cut(s) 1088
BtrI CACGTC 1 cut(s) 1931
BtsCI GGATG 3 cut(s) 96, 897, 1643
BtsIMutI CAGTG 4 cut(s) 326, 630, 1104, 1549
BtuMI TCGCGA 1 cut(s) 387
Cac8I GCNNGC 4 cut(s) 108, 242, 470, 1123
CciI TCATGA 2 cut(s) 780, 1774
CfoI GCGC 2 cut(s) 379, 468
Cfr13I GGNCC 4 cut(s) 1112, 1539, 1729, 1919
ClaI ATCGAT 1 cut(s) 864
CseI GACGC 1 cut(s) 1474
Csp6I GTAC 5 cut(s) 134, 141, 197, 1105, 1366
CviQI GTAC 5 cut(s) 134, 141, 197, 1105, 1366
DdeI CTNAG 7 cut(s) 453, 651, 749, 876, 902, 1052, 1649
Eam1104I CTCTTC 2 cut(s) 18, 83
EarI CTCTTC 2 cut(s) 18, 83
Eco130I CCWWGG 1 cut(s) 1242
Eco147I AGGCCT 2 cut(s) 1247, 1769
Eco31I GGTCTC 1 cut(s) 1693
Eco32I GATATC 1 cut(s) 1069
Eco47I GGWCC 4 cut(s) 1112, 1539, 1729, 1919
Eco57I CTGAAG 1 cut(s) 1094
Eco81I CCTNAGG 1 cut(s) 902
Eco88I CYCGRG 1 cut(s) 287
EcoNI CCTNNNNNAGG 1 cut(s) 1119
EcoRI GAATTC 1 cut(s) 362
EcoRII CCWGG 2 cut(s) 247, 372
EcoRV GATATC 1 cut(s) 1069
EcoT14I CCWWGG 1 cut(s) 1242
EcoT22I ATGCAT 1 cut(s) 530
ErhI CCWWGG 1 cut(s) 1242
Esp3I CGTCTC 1 cut(s) 949
FalI AAGNNNNNCTT 1 cut(s) 34
FaqI GGGAC 2 cut(s) 416, 557
FbaI TGATCA 1 cut(s) 498
FblI GTMKAC 1 cut(s) 1798
Fnu4HI GCNGC 6 cut(s) 339, 380, 1498, 1531, 1807, 1906
FokI GGATG 3 cut(s) 83, 884, 1650
Fsp4HI GCNGC 6 cut(s) 339, 380, 1498, 1531, 1807, 1906
FspBI CTAG 7 cut(s) 335, 669, 695, 938, 1472, 1526, 1794
GlaI GCGC 2 cut(s) 378, 467
GluI GCNGC 6 cut(s) 339, 380, 1498, 1531, 1807, 1906
HaeIII GGCC 5 cut(s) 506, 1247, 1530, 1572, 1769
HapII CCGG 3 cut(s) 605, 1823, 1844
HgaI GACGC 1 cut(s) 1474
HhaI GCGC 2 cut(s) 379, 468
Hin1I GRCGYC 1 cut(s) 942
Hin6I GCGC 2 cut(s) 377, 466
HinP1I GCGC 2 cut(s) 377, 466
HincII GTYRAC 2 cut(s) 1662, 1799
HindII GTYRAC 2 cut(s) 1662, 1799
HindIII AAGCTT 1 cut(s) 1610
HpaII CCGG 3 cut(s) 605, 1823, 1844
HphI GGTGA 6 cut(s) 263, 371, 1189, 1322, 1460, 1555
Hpy166II GTNNAC 7 cut(s) 157, 207, 987, 1095, 1342, 1662, 1799
Hpy188I TCNGA 8 cut(s) 7, 454, 498, 794, 949, 969, 1490, 1591
Hpy8I GTNNAC 7 cut(s) 157, 207, 987, 1095, 1342, 1662, 1799
Hpy99I CGWCG 1 cut(s) 1490
HpyAV CCTTC 6 cut(s) 70, 768, 1258, 1316, 1693, 1720
HpyCH4III ACNGT 5 cut(s) 634, 886, 1099, 1281, 1666
HpyCH4IV ACGT 2 cut(s) 942, 1930
HpyF10VI GCNNNNNNNGC 6 cut(s) 241, 344, 376, 454, 463, 1866
HpyF3I CTNAG 7 cut(s) 453, 651, 749, 876, 902, 1052, 1649
HpySE526I ACGT 2 cut(s) 942, 1930
Hsp92I GRCGYC 1 cut(s) 942
HspAI GCGC 2 cut(s) 377, 466
Kpn2I TCCGGA 1 cut(s) 1843
Ksp22I TGATCA 1 cut(s) 498
LmnI GCTCC 4 cut(s) 375, 1033, 1238, 1316
Lsp1109I GCAGC 5 cut(s) 350, 391, 1509, 1793, 1892
LweI GCATC 4 cut(s) 222, 332, 1074, 1134
MaeI CTAG 7 cut(s) 335, 669, 695, 938, 1472, 1526, 1794
MaeII ACGT 2 cut(s) 942, 1930
MaeIII GTNAC 4 cut(s) 251, 1281, 1672, 1895
MfeI CAATTG 2 cut(s) 411, 1026
MflI RGATCY 1 cut(s) 15
MhlI GDGCHC 3 cut(s) 209, 1344, 1862
MluCI AATT 9 cut(s) 362, 411, 477, 785, 807, 1026, 1150, 1409, 1754
MlyI GAGTC 5 cut(s) 325, 438, 760, 1580, 1856
MmeI TCCRAC 1 cut(s) 1468
Mph1103I ATGCAT 1 cut(s) 530
MroI TCCGGA 1 cut(s) 1843
MroXI GAANNNNTTC 2 cut(s) 788, 1401
MseI TTAA 4 cut(s) 489, 729, 888, 1936
MslI CAYNNNNRTG 4 cut(s) 51, 275, 1290, 1914
MspI CCGG 3 cut(s) 605, 1823, 1844
MspR9I CCNGG 3 cut(s) 249, 374, 1824
MunI CAATTG 2 cut(s) 411, 1026
Mva1269I GAATGC 1 cut(s) 530
MvaI CCWGG 2 cut(s) 249, 374
MvnI CGCG 1 cut(s) 387
MwoI GCNNNNNNNGC 6 cut(s) 241, 344, 376, 454, 463, 1866
NciI CCSGG 1 cut(s) 1824
NlaIV GGNNCC 5 cut(s) 371, 1240, 1312, 1842, 1859
NmuCI GTSAC 3 cut(s) 251, 1281, 1672
NruI TCGCGA 1 cut(s) 387
NsiI ATGCAT 1 cut(s) 530
NspI RCATGY 1 cut(s) 155
PaeR7I CTCGAG 1 cut(s) 287
PagI TCATGA 2 cut(s) 780, 1774
PceI AGGCCT 2 cut(s) 1247, 1769
PcsI WCGNNNNNNNCGW 1 cut(s) 948
PctI GAATGC 1 cut(s) 530
PdmI GAANNNNTTC 2 cut(s) 788, 1401
PfeI GAWTC 9 cut(s) 56, 71, 219, 389, 964, 1021, 1225, 1402, 1465
PflFI GACNNNGTC 1 cut(s) 1733
PflMI CCANNNNNTGG 1 cut(s) 1879
PkrI GCNGC 6 cut(s) 340, 381, 1499, 1532, 1808, 1907
PleI GAGTC 5 cut(s) 325, 437, 760, 1580, 1855
PpsI GAGTC 5 cut(s) 325, 437, 760, 1580, 1855
Psp6I CCWGG 2 cut(s) 247, 372
PspGI CCWGG 2 cut(s) 247, 372
PspN4I GGNNCC 5 cut(s) 371, 1240, 1312, 1842, 1859
PspPI GGNCC 4 cut(s) 1112, 1539, 1729, 1919
PsuI RGATCY 1 cut(s) 15
PsyI GACNNNGTC 1 cut(s) 1733
RruI TCGCGA 1 cut(s) 387
RsaI GTAC 5 cut(s) 135, 142, 198, 1106, 1367
RsaNI GTAC 5 cut(s) 134, 141, 197, 1105, 1366
RseI CAYNNNNRTG 4 cut(s) 51, 275, 1290, 1914
SalI GTCGAC 1 cut(s) 1797
SaqAI TTAA 4 cut(s) 489, 729, 888, 1936
SatI GCNGC 6 cut(s) 339, 380, 1498, 1531, 1807, 1906
Sau96I GGNCC 4 cut(s) 1112, 1539, 1729, 1919
SchI GAGTC 5 cut(s) 325, 438, 760, 1580, 1856
ScrFI CCNGG 3 cut(s) 249, 374, 1824
SduI GDGCHC 3 cut(s) 209, 1344, 1862
SfaNI GCATC 4 cut(s) 222, 332, 1074, 1134
SfcI CTRYAG 1 cut(s) 1167
Sfr274I CTCGAG 1 cut(s) 287
SinI GGWCC 4 cut(s) 1112, 1539, 1729, 1919
SlaI CTCGAG 1 cut(s) 287
SmiMI CAYNNNNRTG 4 cut(s) 51, 275, 1290, 1914
SmlI CTYRAG 5 cut(s) 101, 144, 287, 443, 482
SmoI CTYRAG 5 cut(s) 101, 144, 287, 443, 482
SpeI ACTAGT 1 cut(s) 668
Sse9I AATT 9 cut(s) 362, 411, 477, 785, 807, 1026, 1150, 1409, 1754
SseBI AGGCCT 2 cut(s) 1247, 1769
SsiI CCGC 4 cut(s) 1102, 1383, 1531, 1643
SspMI CTAG 7 cut(s) 335, 669, 695, 938, 1472, 1526, 1794
StuI AGGCCT 2 cut(s) 1247, 1769
StyD4I CCNGG 3 cut(s) 247, 372, 1822
StyI CCWWGG 1 cut(s) 1242
TaaI ACNGT 5 cut(s) 634, 886, 1099, 1281, 1666
TaiI ACGT 2 cut(s) 945, 1933
TasI AATT 9 cut(s) 362, 411, 477, 785, 807, 1026, 1150, 1409, 1754
TatI WGTACW 2 cut(s) 133, 1365
TauI GCSGC 1 cut(s) 1533
TfiI GAWTC 9 cut(s) 56, 71, 219, 389, 964, 1021, 1225, 1402, 1465
Tru1I TTAA 4 cut(s) 489, 729, 888, 1936
Tru9I TTAA 4 cut(s) 489, 729, 888, 1936
TscAI CASTG 4 cut(s) 333, 637, 1104, 1549
TseFI GTSAC 3 cut(s) 251, 1281, 1672
TseI GCWGC 5 cut(s) 338, 379, 1497, 1806, 1905
Tsp45I GTSAC 3 cut(s) 251, 1281, 1672
TspDTI ATGAA 6 cut(s) 69, 297, 797, 916, 1123, 1196
TspGWI ACGGA 1 cut(s) 1303
TspRI CASTG 4 cut(s) 333, 637, 1104, 1549
Tth111I GACNNNGTC 1 cut(s) 1733
Van91I CCANNNNNTGG 1 cut(s) 1879
VneI GTGCAC 2 cut(s) 205, 1340
VpaK11BI GGWCC 4 cut(s) 1112, 1539, 1729, 1919
XagI CCTNNNNNAGG 1 cut(s) 1119
XapI RAATTY 3 cut(s) 362, 1150, 1754
XceI RCATGY 1 cut(s) 155
XhoI CTCGAG 1 cut(s) 287
XmiI GTMKAC 1 cut(s) 1798
XmnI GAANNNNTTC 2 cut(s) 788, 1401
XspI CTAG 7 cut(s) 335, 669, 695, 938, 1472, 1526, 1794
ZraI GACGTC 1 cut(s) 943
Zsp2I ATGCAT 1 cut(s) 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.