FvH4_4g18590

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0000950
Physical Location & Seq
Forward (+)
22449100 .. 22455072
5973 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g18590.t1

Sequence Viewer

Length: 2010 bp
ATGGAGAGAGTGAAAGAGTGGTTCCGAAGGTACCTCTACCGCTCCAAGGCTACAATATGCATTCGAGGTTTAATTTTCAGCCACAGCCTCGTAAACCATGCGATAGTGGCTATCTTGATAGTGTACCTCACTTCCAATTGGCAGAATCAACATTTTCAAATAGCTGCTGTAGTTACAAATCTGCAAGATGGGTTTTCAGACATCATTGTGATCTTCTTAGCTCATTTCTCCAGCACCTACAGCGGTTCTTTTAATTTAATTCTCACCACAAACGCTGCCTATATTGCTGGACTGGCTATACTATGGCGTTCTACTGAGCATATGACCACAGATACAGCTGCAAAGGTATACTACGGAGCTGTTTTACTGCTTTTATTAGGCGAAGCAGGGCGGTCGGCTCTCTTGCATGAATTTCTTGATATGCAATATTTAAGGGAACATAACCAATCAGAAATCATAGAACCTGAAGAACTACGAGAGCGTCAAGATGCTTTATGGGATCTTCCCTGGTTGTTGGGTGCAGTCGTACCACTTCTTCTTGTAAAAACAAATTGGGAACAAATTTTCATGATTTCAACAATTGTGATGGGAGTGTTTTACTTATTCTTCTGTTGTGGCTACTCCTATTATTTCATAAATAGATTGGGAGAGACCATGTATCCAATCGACGAAGCAGAAAGGGAGGAAAGTGGCACAGACAAAAAAGTGGATGACATAGACCATTTACAAGGGCAGAATATTCGCCAAGACGAAGAGGAGCTCGCAGGACCAGCAAGAGGGAATATTTGGAGATTGATAAAAAGAATGAATTTGACAGGGGAAAAGCATTTTCGCAAACAAATAATCTCCGTCTGGATAACCTTCCTAGCATACAGCATTGTTAGTGCGGCAGGAAGTACGTTCTTTTATGAACAAATGAGTAACTTGCGTATTCCAATCAAGAACTATGACATTGCAGTTTATTTCAATGTGATTGCTTCACTTTCAAAGCACGCGATTTCATCTCTTTGGGAATGGAAAATTCCAAAGGAAACGAAAACGCGAGTTAATACGCTGGTGAGAATCGGTTGTGGACTGGCTTGTGTTGTGACGTGTATCGCGACGGCTTGGCGGGTTGAGATCAGGAGGTTGAAGGAAGTTCAAAGGCAAGGAGTTGGAGACACTTCAGACGTTGTTCCCATGAGTATCTTTAGGCTAGTTCCTCAGTTCTCCTTGTTGGGGATCATGGAAGGGCTCTTAGTTGATGGGTTGATTGACTTTTTTGTTGAGCGAGTGGCAGGTTCTGATGACAAAGTGATGGTCGAATACTACGCATCCCACACCAGCGACGTCGTCATAGGTGTTGGAAAGTTACTGACGGCACTTAGCATTTTGGCGTTTAAAAGAAGATGGTTTGATGACACCATAAATATGAGCCGTTTGGACAAGTATTACAGAGCATTAACATTCATGAGTCTCGTTGGATTTTGCTACTACGTGTGTGTCTCCACCATGTATTACACCAATGCTGATGTACAAGAATCTACCGAACATAAAGAAAATGAGACCAACTCGCACAGAAATGAGAAGCATGAGTCCTCACCGCCTTTACCACATAAGTCCTCACCGCCTTCACCACAAGAGCCGTCACAGGCATTACAGGATTTACAGCAAGAACCACAAGACTTCCTTTACCATCAGCAGCCTCCTCCTCCTCCGCAGCAGCAACCACCGCCGCTGCCACCTCAATATTATCTGCCCCCACAGTACCCACCACCACAACAAGATCACTCTCCCGAGCAAACATACCCACCGGCAACGTCATTCCAACCACAGCCCTATCCGCCGCGGCCGTACAACTCATACACATATGCACCGCAGCCGGGCATGCCTGCAGAGTCGTACCCACCACAGCCATACCAGCCGCAGACATACAACTCGTACCCAACGCGGTCGGGTGTGCCTCCACAGCTGTACACACCACAGCCTTACTCGCTTCCATATAGCCCACAGCAGTACCCGCCACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

670

Amino Acids

76.79

Weight (kDa)

5.89

Isoelectric Point (pI)

57.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1332
Acc36I ACCTGC 1 cut(s) 1268
Acc65I GGTACC 1 cut(s) 30
AccB1I GGYRCC 1 cut(s) 30
AccBSI CCGCTC 1 cut(s) 42
AccI GTMKAC 1 cut(s) 348
AccII CGCG 5 cut(s) 995, 1042, 1100, 1828, 1930
AclWI GGATC 2 cut(s) 507, 1229
AcoI YGGCCR 1 cut(s) 1829
AcsI RAATTY 4 cut(s) 410, 561, 808, 1020
AcuI CTGAAG 2 cut(s) 486, 1149
AcyI GRCGYC 1 cut(s) 1329
AfiI CCNNNNNNNGG 4 cut(s) 46, 776, 1218, 1862
AflIII ACRYGT 2 cut(s) 1091, 1476
AgsI TTSAA 6 cut(s) 158, 576, 967, 987, 1132, 1142
AjiI CACGTC 1 cut(s) 1092
AjnI CCWGG 1 cut(s) 506
AluBI AGCT 6 cut(s) 164, 221, 338, 359, 760, 1951
AluI AGCT 6 cut(s) 164, 221, 338, 359, 760, 1951
Alw21I GWGCWC 1 cut(s) 762
Alw26I GTCTC 5 cut(s) 644, 1152, 1460, 1489, 1538
AlwI GGATC 2 cut(s) 507, 1229
AlwNI CAGNNNCTG 1 cut(s) 1283
Ama87I CYCGRG 1 cut(s) 1775
AoxI GGCC 1 cut(s) 1829
ApeKI GCWGC 8 cut(s) 164, 275, 338, 1681, 1699, 1702, 1717, 1858
ApoI RAATTY 4 cut(s) 410, 561, 808, 1020
ArsI GACNNNNNNTTYG 2 cut(s) 1284, 1316
Asp718I GGTACC 1 cut(s) 30
AspS9I GGNCC 1 cut(s) 767
AsuC2I CCSGG 1 cut(s) 1863
AsuHPI GGTGA 5 cut(s) 256, 1069, 1572, 1596, 1605
AvaI CYCGRG 1 cut(s) 1775
AvaII GGWCC 1 cut(s) 767
BaeI ACNNNNGTAYC 1 cut(s) 1979
BanI GGYRCC 1 cut(s) 30
BanII GRGCYC 2 cut(s) 762, 1236
BarI GAAGNNNNNNTAC 2 cut(s) 519, 551
Bbv12I GWGCWC 1 cut(s) 762
BbvI GCAGC 8 cut(s) 151, 262, 325, 1693, 1704, 1711, 1714, 1870
BccI CCATC 6 cut(s) 182, 580, 1238, 1291, 1383, 1683
BceAI ACGGC 5 cut(s) 1119, 1374, 1401, 1609, 1816
BcgI CGANNNNNNTGC 4 cut(s) 375, 409, 722, 756
BciT130I CCWGG 1 cut(s) 508
BciVI GTATCC 1 cut(s) 669
BcnI CCSGG 1 cut(s) 1863
BcoDI GTCTC 5 cut(s) 644, 1152, 1460, 1489, 1538
BfaI CTAG 2 cut(s) 866, 1196
BfmI CTRYAG 3 cut(s) 168, 238, 1872
BfuAI ACCTGC 1 cut(s) 1268
BfuI GTATCC 1 cut(s) 669
Bme1390I CCNGG 2 cut(s) 508, 1863
Bme18I GGWCC 1 cut(s) 767
BmeT110I CYCGRG 1 cut(s) 1775
BmgBI CACGTC 1 cut(s) 1092
BmgT120I GGNCC 1 cut(s) 767
BmiI GGNNCC 2 cut(s) 23, 32
BmrFI CCNGG 2 cut(s) 508, 1863
BmsI GCATC 2 cut(s) 478, 1322
BplI GAGNNNNNCTC 2 cut(s) 1535, 1567
BpmI CTGGAG 1 cut(s) 214
BpuMI CCSGG 1 cut(s) 1863
BsaAI YACGTR 1 cut(s) 1477
BsaHI GRCGYC 1 cut(s) 1329
BsaI GGTCTC 2 cut(s) 644, 1538
BsaJI CCNNGG 3 cut(s) 45, 506, 1826
Bsc4I CCNNNNNNNGG 4 cut(s) 46, 776, 1218, 1862
Bse118I RCCGGY 1 cut(s) 1792
Bse1I ACTGG 2 cut(s) 297, 1080
Bse3DI GCAATG 1 cut(s) 951
BseBI CCWGG 1 cut(s) 508
BseDI CCNNGG 3 cut(s) 45, 506, 1826
BseGI GGATG 2 cut(s) 715, 1313
BseLI CCNNNNNNNGG 4 cut(s) 46, 776, 1218, 1862
BseMI GCAATG 1 cut(s) 951
BseMII CTCAG 2 cut(s) 306, 1217
BseNI ACTGG 2 cut(s) 297, 1080
BseRI GAGGAG 4 cut(s) 770, 1677, 1680, 1683
BseX3I CGGCCG 1 cut(s) 1829
BseXI GCAGC 8 cut(s) 151, 262, 325, 1693, 1704, 1711, 1714, 1870
BsgI GTGCAG 1 cut(s) 540
Bsh1236I CGCG 5 cut(s) 995, 1042, 1100, 1828, 1930
Bsh1285I CGRYCG 3 cut(s) 395, 1832, 1934
BshFI GGCC 1 cut(s) 1831
BshNI GGYRCC 1 cut(s) 30
BsiEI CGRYCG 3 cut(s) 395, 1832, 1934
BsiHKAI GWGCWC 1 cut(s) 762
BsiHKCI CYCGRG 1 cut(s) 1775
BsiSI CCGG 2 cut(s) 1793, 1862
BslI CCNNNNNNNGG 4 cut(s) 46, 776, 1218, 1862
BsmAI GTCTC 5 cut(s) 644, 1152, 1460, 1489, 1538
BsmI GAATGC 1 cut(s) 60
BsnI GGCC 1 cut(s) 1831
Bso31I GGTCTC 2 cut(s) 644, 1538
BsoBI CYCGRG 1 cut(s) 1775
Bsp1286I GDGCHC 2 cut(s) 762, 1236
Bsp1407I TGTACA 2 cut(s) 1513, 1953
Bsp143I GATC 5 cut(s) 210, 499, 1119, 1221, 1765
Bsp68I TCGCGA 1 cut(s) 1100
BspANI GGCC 1 cut(s) 1831
BspCNI CTCAG 2 cut(s) 307, 1216
BspFNI CGCG 5 cut(s) 995, 1042, 1100, 1828, 1930
BspHI TCATGA 2 cut(s) 567, 1449
BspLI GGNNCC 2 cut(s) 23, 32
BspMAI CTGCAG 1 cut(s) 1876
BspMI ACCTGC 1 cut(s) 1268
BspPI GGATC 2 cut(s) 507, 1229
BspT107I GGYRCC 1 cut(s) 30
BspTNI GGTCTC 2 cut(s) 644, 1538
BsrBI CCGCTC 1 cut(s) 42
BsrDI GCAATG 1 cut(s) 951
BsrFI RCCGGY 1 cut(s) 1792
BsrGI TGTACA 2 cut(s) 1513, 1953
BsrI ACTGG 2 cut(s) 297, 1080
BssAI RCCGGY 1 cut(s) 1792
BssECI CCNNGG 3 cut(s) 45, 506, 1826
BssMI GATC 5 cut(s) 210, 499, 1119, 1221, 1765
BssNAI GTATAC 1 cut(s) 349
BssNI GRCGYC 1 cut(s) 1329
BssT1I CCWWGG 1 cut(s) 45
Bst1107I GTATAC 1 cut(s) 349
Bst2UI CCWGG 1 cut(s) 508
Bst4CI ACNGT 1 cut(s) 1746
Bst6I CTCTTC 1 cut(s) 747
BstACI GRCGYC 1 cut(s) 1329
BstAUI TGTACA 2 cut(s) 1513, 1953
BstBAI YACGTR 1 cut(s) 1477
BstC8I GCNNGC 4 cut(s) 762, 993, 1868, 1872
BstDEI CTNAG 5 cut(s) 217, 315, 1203, 1237, 1364
BstDSI CCRYGG 1 cut(s) 1826
BstF5I GGATG 2 cut(s) 715, 1313
BstFNI CGCG 5 cut(s) 995, 1042, 1100, 1828, 1930
BstKTI GATC 5 cut(s) 213, 502, 1122, 1224, 1768
BstMAI GTCTC 5 cut(s) 644, 1152, 1460, 1489, 1538
BstMBI GATC 5 cut(s) 210, 499, 1119, 1221, 1765
BstMCI CGRYCG 3 cut(s) 395, 1832, 1934
BstNI CCWGG 1 cut(s) 508
BstNSI RCATGY 1 cut(s) 1870
BstSCI CCNGG 2 cut(s) 506, 1861
BstSFI CTRYAG 3 cut(s) 168, 238, 1872
BstUI CGCG 5 cut(s) 995, 1042, 1100, 1828, 1930
BstV1I GCAGC 8 cut(s) 151, 262, 325, 1693, 1704, 1711, 1714, 1870
BstX2I RGATCY 1 cut(s) 499
BstYI RGATCY 1 cut(s) 499
BstZ17I GTATAC 1 cut(s) 349
BstZI CGGCCG 1 cut(s) 1829
BsuI GTATCC 1 cut(s) 669
BsuRI GGCC 1 cut(s) 1831
BtgI CCRYGG 1 cut(s) 1826
BtrI CACGTC 1 cut(s) 1092
BtsCI GGATG 2 cut(s) 715, 1313
BtuMI TCGCGA 1 cut(s) 1100
BveI ACCTGC 1 cut(s) 1268
Cac8I GCNNGC 4 cut(s) 762, 993, 1868, 1872
CaiI CAGNNNCTG 1 cut(s) 1283
CciI TCATGA 2 cut(s) 567, 1449
Cfr10I RCCGGY 1 cut(s) 1792
Cfr13I GGNCC 1 cut(s) 767
Cfr42I CCGCGG 1 cut(s) 1829
CseI GACGC 1 cut(s) 470
DdeI CTNAG 5 cut(s) 217, 315, 1203, 1237, 1364
DpnI GATC 5 cut(s) 212, 501, 1121, 1223, 1767
DpnII GATC 5 cut(s) 210, 499, 1119, 1221, 1765
DraI TTTAAA 1 cut(s) 1381
EaeI YGGCCR 1 cut(s) 1829
EagI CGGCCG 1 cut(s) 1829
Eam1104I CTCTTC 1 cut(s) 747
EarI CTCTTC 1 cut(s) 747
EciI GGCGGA 1 cut(s) 1812
Ecl136II GAGCTC 1 cut(s) 760
EclXI CGGCCG 1 cut(s) 1829
Eco130I CCWWGG 1 cut(s) 45
Eco24I GRGCYC 2 cut(s) 762, 1236
Eco31I GGTCTC 2 cut(s) 644, 1538
Eco47I GGWCC 1 cut(s) 767
Eco52I CGGCCG 1 cut(s) 1829
Eco53kI GAGCTC 1 cut(s) 760
Eco57I CTGAAG 2 cut(s) 486, 1149
Eco88I CYCGRG 1 cut(s) 1775
EcoICRI GAGCTC 1 cut(s) 760
EcoRII CCWGG 1 cut(s) 506
EcoT14I CCWWGG 1 cut(s) 45
EcoT22I ATGCAT 1 cut(s) 62
EcoT38I GRGCYC 2 cut(s) 762, 1236
ErhI CCWWGG 1 cut(s) 45
FalI AAGNNNNNCTT 2 cut(s) 1653, 1685
FauI CCCGC 2 cut(s) 1104, 2007
FauNDI CATATG 2 cut(s) 321, 1849
FblI GTMKAC 1 cut(s) 348
FokI GGATG 2 cut(s) 722, 1300
FriOI GRGCYC 2 cut(s) 762, 1236
FspBI CTAG 2 cut(s) 866, 1196
GsuI CTGGAG 1 cut(s) 214
HaeIII GGCC 1 cut(s) 1831
HapII CCGG 2 cut(s) 1793, 1862
HgaI GACGC 1 cut(s) 470
Hin1I GRCGYC 1 cut(s) 1329
HinfI GANTC 6 cut(s) 145, 1062, 1453, 1520, 1574, 1877
HpaII CCGG 2 cut(s) 1793, 1862
HphI GGTGA 5 cut(s) 256, 1069, 1572, 1596, 1605
Hpy166II GTNNAC 5 cut(s) 94, 124, 349, 1073, 1956
Hpy188I TCNGA 5 cut(s) 26, 199, 451, 1168, 1285
Hpy8I GTNNAC 5 cut(s) 94, 124, 349, 1073, 1956
Hpy99I CGWCG 4 cut(s) 671, 1105, 1331, 1334
HpyAV CCTTC 5 cut(s) 21, 871, 1126, 1223, 1620
HpyCH4III ACNGT 1 cut(s) 1746
HpyCH4IV ACGT 6 cut(s) 899, 1091, 1170, 1329, 1476, 1799
HpyCH4V TGCA 9 cut(s) 60, 184, 341, 406, 424, 521, 956, 1853, 1874
HpyF3I CTNAG 5 cut(s) 217, 315, 1203, 1237, 1364
HpySE526I ACGT 6 cut(s) 899, 1091, 1170, 1329, 1476, 1799
Hsp92I GRCGYC 1 cut(s) 1329
KpnI GGTACC 1 cut(s) 34
KspI CCGCGG 1 cut(s) 1829
Kzo9I GATC 5 cut(s) 210, 499, 1119, 1221, 1765
LmnI GCTCC 3 cut(s) 47, 356, 757
Lsp1109I GCAGC 8 cut(s) 151, 262, 325, 1693, 1704, 1711, 1714, 1870
LweI GCATC 2 cut(s) 478, 1322
MaeI CTAG 2 cut(s) 866, 1196
MaeII ACGT 6 cut(s) 899, 1091, 1170, 1329, 1476, 1799
MaeIII GTNAC 5 cut(s) 172, 920, 1087, 1350, 1626
MalI GATC 5 cut(s) 212, 501, 1121, 1223, 1767
MbiI CCGCTC 1 cut(s) 42
MboI GATC 5 cut(s) 210, 499, 1119, 1221, 1765
MboII GAAGA 7 cut(s) 205, 479, 494, 527, 598, 764, 1398
MfeI CAATTG 2 cut(s) 136, 579
MflI RGATCY 1 cut(s) 499
MhlI GDGCHC 2 cut(s) 762, 1236
MlyI GAGTC 3 cut(s) 1462, 1583, 1886
MmeI TCCRAC 4 cut(s) 1135, 1324, 1441, 1831
Mph1103I ATGCAT 1 cut(s) 62
MseI TTAA 7 cut(s) 71, 252, 257, 431, 1047, 1380, 1442
MslI CAYNNNNRTG 3 cut(s) 206, 1409, 1560
MspA1I CMGCKG 5 cut(s) 243, 338, 1717, 1828, 1951
MspI CCGG 2 cut(s) 1793, 1862
MspR9I CCNGG 2 cut(s) 508, 1863
MunI CAATTG 2 cut(s) 136, 579
Mva1269I GAATGC 1 cut(s) 60
MvaI CCWGG 1 cut(s) 508
MvnI CGCG 5 cut(s) 995, 1042, 1100, 1828, 1930
NciI CCSGG 1 cut(s) 1863
NdeI CATATG 2 cut(s) 321, 1849
NdeII GATC 5 cut(s) 210, 499, 1119, 1221, 1765
NlaIV GGNNCC 2 cut(s) 23, 32
NmuCI GTSAC 2 cut(s) 1087, 1626
NruI TCGCGA 1 cut(s) 1100
NsiI ATGCAT 1 cut(s) 62
NspI RCATGY 1 cut(s) 1870
PaeI GCATGC 1 cut(s) 1870
PagI TCATGA 2 cut(s) 567, 1449
PcsI WCGNNNNNNNCGW 1 cut(s) 1097
PctI GAATGC 1 cut(s) 60
PfeI GAWTC 3 cut(s) 145, 1062, 1520
PflFI GACNNNGTC 1 cut(s) 1331
PleI GAGTC 3 cut(s) 1461, 1582, 1885
PpsI GAGTC 3 cut(s) 1461, 1582, 1885
Ppu21I YACGTR 1 cut(s) 1477
Psp124BI GAGCTC 1 cut(s) 762
Psp6I CCWGG 1 cut(s) 506
PspGI CCWGG 1 cut(s) 506
PspN4I GGNNCC 2 cut(s) 23, 32
PspPI GGNCC 1 cut(s) 767
PstI CTGCAG 1 cut(s) 1876
PstNI CAGNNNCTG 1 cut(s) 1283
PsuI RGATCY 1 cut(s) 499
PsyI GACNNNGTC 1 cut(s) 1331
PvuII CAGCTG 2 cut(s) 338, 1951
RruI TCGCGA 1 cut(s) 1100
RseI CAYNNNNRTG 3 cut(s) 206, 1409, 1560
SacI GAGCTC 1 cut(s) 762
SacII CCGCGG 1 cut(s) 1829
SaqAI TTAA 7 cut(s) 71, 252, 257, 431, 1047, 1380, 1442
Sau3AI GATC 5 cut(s) 210, 499, 1119, 1221, 1765
Sau96I GGNCC 1 cut(s) 767
SchI GAGTC 3 cut(s) 1462, 1583, 1886
ScrFI CCNGG 2 cut(s) 508, 1863
SduI GDGCHC 2 cut(s) 762, 1236
SfaNI GCATC 2 cut(s) 478, 1322
SfcI CTRYAG 3 cut(s) 168, 238, 1872
Sfr303I CCGCGG 1 cut(s) 1829
SgrBI CCGCGG 1 cut(s) 1829
SinI GGWCC 1 cut(s) 767
SmiMI CAYNNNNRTG 3 cut(s) 206, 1409, 1560
SphI GCATGC 1 cut(s) 1870
SspI AATATT 4 cut(s) 428, 739, 784, 1730
SspMI CTAG 2 cut(s) 866, 1196
SstI GAGCTC 1 cut(s) 762
StyD4I CCNGG 2 cut(s) 506, 1861
StyI CCWWGG 1 cut(s) 45
TaaI ACNGT 1 cut(s) 1746
TaiI ACGT 6 cut(s) 902, 1094, 1173, 1332, 1479, 1802
TaqI TCGA 3 cut(s) 64, 666, 1302
TatI WGTACW 2 cut(s) 1513, 1953
TauI GCSGC 5 cut(s) 890, 1717, 1828, 1831, 1906
TfiI GAWTC 3 cut(s) 145, 1062, 1520
Tru1I TTAA 7 cut(s) 71, 252, 257, 431, 1047, 1380, 1442
Tru9I TTAA 7 cut(s) 71, 252, 257, 431, 1047, 1380, 1442
TseFI GTSAC 2 cut(s) 1087, 1626
TseI GCWGC 8 cut(s) 164, 275, 338, 1681, 1699, 1702, 1717, 1858
Tsp45I GTSAC 2 cut(s) 1087, 1626
TspDTI ATGAA 7 cut(s) 423, 556, 622, 821, 924, 990, 1438
TspGWI ACGGA 2 cut(s) 369, 838
Tth111I GACNNNGTC 1 cut(s) 1331
VpaK11BI GGWCC 1 cut(s) 767
XapI RAATTY 4 cut(s) 410, 561, 808, 1020
XceI RCATGY 1 cut(s) 1870
XmiI GTMKAC 1 cut(s) 348
XspI CTAG 2 cut(s) 866, 1196
ZraI GACGTC 1 cut(s) 1330
Zsp2I ATGCAT 1 cut(s) 62
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.