Rh4AG245300

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000950
Physical Location & Seq
Reverse (-)
55592752 .. 55607652
14901 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG245300.1

Sequence Viewer

Length: 1749 bp
ATGAAGAACTTTGACTCTCTAGCAGTCCATCTCGAAATCGAGAGAATCAAAGACCGTTTGGTCTTGAGCCATAGCTTCGTAAACCATGTGGTAATATCTGTATTGATAACGTACCTCACGTCCGAAATTTGGAAGCCATCAAATGATAATTATCAGCTGGCTGCTGTAGCTATAAATATGCTAGATGGGGTATCATCCATCATGGTGTGTGTCCTTGCTTTCATCTCAAACAGGGTCGGTCCTTTTAAGGTGATTGCCACCACGAATGCTGCCTATATTCTTGGCCTGGTTCTATTGTGGAGTTCATTTAATTATGCGGGCCGGAAAGCCAGCGCATCTGATAAAGTATTCTACGGAGCTGGCCTACTGTTAGCATTAGGCGAGGCTGGACGGCCGGCTGCTTTGGGTAAATTTCTCGACGACCAATATTTAAGTGAAGTTGATCACAAGATCAAATTACCCGAAACAAAAAGTATTGAAGAAGAAGATCATATTTCGACCAGAAACGAAGAACAAGAAGATCATATTTCGACCAGAAAAGAAGAAGATGACAAGCAAAAACGAGTGGAAGAGGACAAGCAAAAACGTGGGGAAACCCGTCAAACCGCTTTATGGAGCCATCCCTGGTTCATAGGTGCGGCTGCACCTCTTGCCCTTGTAAATAGATCTTGGAAAACTCAACTACTGCTCTCAATGATCGCGGTGGGAGTTTCTTACTTATTGTTCTGGTATGGCGTCTTCGCGTATTCCAGAAATATCAGAAATGAATCAAATGATCCAGCAGTAGAAGATGCACCAAGAGATCCTGGACTTTGCCAAGCCATCAAAAGTCTCTCACCACCAGAAAAAACAGCAAGCTTGAAGTTTTGGCTCAAACCCAATTCCAGAAAACGTTATTACAAAGAAATGCTAGTCATGTGCTTAGCCTTCATCGTATACAGTCTGGTGGAGGCAACGGGGAGCACCTTCTTTTTCAATCAAATGAGTCTACTGAACTATCACATCGGGAACTTTGAATTGCCCATCATATATTTCGTTATACTCCAATCCTTGTCAAGCTCCGTAATCTCATTTCTCTGGAGGTTACTAGTTCCAAATCAAGAGTCAAAACGTGCTACGTTAACGTTGGTGAGGATCGGCTGTGGTTTAGTTTGCTCTGTGCTATGTATGGTTGCTGCTTGGCAAGTTGAGATTCGTAGGAAAATTAAGATCAATACAGAATATAAAGGTGACCCCGAGATGGAAATTTCCATGAGCATTCTTTGGTTAATTCCACAATTCTCCCTGTTAGGGTTTATGAGAGGGTTAGCGGCAGATGGATTGATCGACTTTTTTGCGGATCGAGTTGATGGTAATGGTAAAGGTAAAAGAAAGGCTAGGTATTATGGATCCTACGCCAGTGAATTAGTCCTGGGTATTGGAACACTACTAACCGCCGTTAGTATTTTCGGGTTTAGACGAACTTGGTTGGTTAATGATATATACAAGAATCGTCTGGATAAATACTACAGGGGACTAGTATTCATAGGTGTTGCTAACCTTTGCTATTACTTCTGTGTCGCCGTTTACTTTTATACCAATAAGGAGGATAACTCGCAAGTAAGTTGTGCCGCAGATGGAAATGAACAAAGTGAAATGCCGGACGAGGACAAAAAATATTATCATCACGAGGTGTCCTCAGGTGGTGCGGAGACTCCGGACTTGGAAGAAATGAAGTCGAGTTTTGATTGTTGTAGTAGTTTCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

582

Amino Acids

65.88

Weight (kDa)

6.58

Isoelectric Point (pI)

43.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 301 - 447 3.8e-09 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 59
AccI GTMKAC 2 cut(s) 936, 988
AccII CGCG 2 cut(s) 701, 743
AccIII TCCGGA 1 cut(s) 1696
AciI CCGC 9 cut(s) 317, 606, 638, 701, 1310, 1337, 1434, 1611, 1688
AclI AACGTT 2 cut(s) 892, 1124
AclWI GGATC 6 cut(s) 770, 797, 1142, 1347, 1383, 1396
AcoI YGGCCR 1 cut(s) 392
AcsI RAATTY 3 cut(s) 126, 410, 1245
AcyI GRCGYC 1 cut(s) 735
AdeI CACNNNGTG 1 cut(s) 1672
AfaI GTAC 1 cut(s) 113
AfiI CCNNNNNNNGG 4 cut(s) 129, 612, 1240, 1290
AgsI TTSAA 4 cut(s) 479, 862, 976, 1016
AhlI ACTAGT 2 cut(s) 1087, 1516
AjiI CACGTC 1 cut(s) 120
AjnI CCWGG 4 cut(s) 285, 623, 805, 1410
AluBI AGCT 6 cut(s) 75, 157, 170, 359, 858, 1059
AluI AGCT 6 cut(s) 75, 157, 170, 359, 858, 1059
Alw21I GWGCWC 1 cut(s) 965
Alw26I GTCTC 2 cut(s) 836, 1685
AlwI GGATC 6 cut(s) 770, 797, 1142, 1347, 1383, 1396
Ama87I CYCGRG 1 cut(s) 1235
Aor13HI TCCGGA 1 cut(s) 1696
AoxI GGCC 4 cut(s) 283, 319, 361, 392
ApeKI GCWGC 5 cut(s) 161, 269, 398, 641, 1175
ApoI RAATTY 3 cut(s) 126, 410, 1245
ArsI GACNNNNNNTTYG 2 cut(s) 1088, 1120
AspLEI GCGC 1 cut(s) 335
AspS9I GGNCC 2 cut(s) 239, 319
AsuHPI GGTGA 4 cut(s) 262, 828, 1141, 1241
AvaI CYCGRG 1 cut(s) 1235
AvaII GGWCC 1 cut(s) 239
AxyI CCTNAGG 1 cut(s) 1678
BamHI GGATCC 1 cut(s) 1388
BarI GAAGNNNNNNTAC 2 cut(s) 722, 754
BauI CACGAG 1 cut(s) 1667
BbsI GAAGAC 1 cut(s) 730
Bbv12I GWGCWC 1 cut(s) 965
BbvI GCAGC 5 cut(s) 148, 256, 385, 628, 1162
BceAI ACGGC 3 cut(s) 407, 1421, 1547
BcgI CGANNNNNNTGC 2 cut(s) 1316, 1350
BciT130I CCWGG 4 cut(s) 287, 625, 807, 1412
BclI TGATCA 1 cut(s) 442
BcoDI GTCTC 2 cut(s) 836, 1685
BcuI ACTAGT 2 cut(s) 1087, 1516
BfaI CTAG 6 cut(s) 20, 182, 911, 1088, 1377, 1517
BfmI CTRYAG 2 cut(s) 165, 1507
BglII AGATCT 1 cut(s) 665
BisI GCNGC 8 cut(s) 162, 270, 399, 639, 642, 1176, 1311, 1611
BlpI GCTNAGC 1 cut(s) 922
BlsI GCNGC 8 cut(s) 163, 271, 400, 640, 643, 1177, 1312, 1612
Bme1390I CCNGG 4 cut(s) 287, 625, 807, 1412
Bme18I GGWCC 1 cut(s) 239
BmeT110I CYCGRG 1 cut(s) 1235
BmgBI CACGTC 1 cut(s) 120
BmgT120I GGNCC 2 cut(s) 239, 319
BmiI GGNNCC 2 cut(s) 617, 1390
BmrFI CCNGG 4 cut(s) 287, 625, 807, 1412
BmsI GCATC 2 cut(s) 344, 781
BpiI GAAGAC 1 cut(s) 730
BplI GAGNNNNNCTC 4 cut(s) 1577, 1609, 1661, 1693
BpmI CTGGAG 1 cut(s) 1099
Bpu1102I GCTNAGC 1 cut(s) 922
BpuEI CTTGAG 1 cut(s) 85
BsaBI GATNNNNATC 1 cut(s) 150
BsaHI GRCGYC 1 cut(s) 735
BsaJI CCNNGG 2 cut(s) 623, 1411
BsaWI WCCGGW 1 cut(s) 1696
Bsc4I CCNNNNNNNGG 4 cut(s) 129, 612, 1240, 1290
Bse118I RCCGGY 1 cut(s) 394
Bse1I ACTGG 1 cut(s) 1398
Bse21I CCTNAGG 1 cut(s) 1678
Bse8I GATNNNNATC 1 cut(s) 150
BseAI TCCGGA 1 cut(s) 1696
BseBI CCWGG 4 cut(s) 287, 625, 807, 1412
BseDI CCNNGG 2 cut(s) 623, 1411
BseGI GGATG 2 cut(s) 194, 619
BseJI GATNNNNATC 1 cut(s) 150
BseLI CCNNNNNNNGG 4 cut(s) 129, 612, 1240, 1290
BseMII CTCAG 1 cut(s) 1692
BseNI ACTGG 1 cut(s) 1398
BseX3I CGGCCG 1 cut(s) 392
BseXI GCAGC 5 cut(s) 148, 256, 385, 628, 1162
BsgI GTGCAG 1 cut(s) 627
Bsh1236I CGCG 2 cut(s) 701, 743
Bsh1285I CGRYCG 1 cut(s) 395
BshFI GGCC 4 cut(s) 285, 321, 363, 394
BsiEI CGRYCG 1 cut(s) 395
BsiHKAI GWGCWC 1 cut(s) 965
BsiHKCI CYCGRG 1 cut(s) 1235
BsiSI CCGG 4 cut(s) 322, 395, 1640, 1697
BslFI GGGAC 1 cut(s) 1527
BslI CCNNNNNNNGG 4 cut(s) 129, 612, 1240, 1290
BsmAI GTCTC 2 cut(s) 836, 1685
BsmFI GGGAC 1 cut(s) 1527
BsmI GAATGC 2 cut(s) 271, 1257
BsnI GGCC 4 cut(s) 285, 321, 363, 394
BsoBI CYCGRG 1 cut(s) 1235
Bsp1286I GDGCHC 1 cut(s) 965
Bsp13I TCCGGA 1 cut(s) 1696
Bsp1720I GCTNAGC 1 cut(s) 922
BspACI CCGC 9 cut(s) 317, 606, 638, 701, 1310, 1337, 1434, 1611, 1688
BspANI GGCC 4 cut(s) 285, 321, 363, 394
BspCNI CTCAG 1 cut(s) 1691
BspEI TCCGGA 1 cut(s) 1696
BspFNI CGCG 2 cut(s) 701, 743
BspLI GGNNCC 2 cut(s) 617, 1390
BspPI GGATC 6 cut(s) 770, 797, 1142, 1347, 1383, 1396
BsrFI RCCGGY 1 cut(s) 394
BsrI ACTGG 1 cut(s) 1398
BssAI RCCGGY 1 cut(s) 394
BssECI CCNNGG 2 cut(s) 623, 1411
BssNAI GTATAC 1 cut(s) 937
BssNI GRCGYC 1 cut(s) 735
BssSI CACGAG 1 cut(s) 1667
Bst1107I GTATAC 1 cut(s) 937
Bst2BI CACGAG 1 cut(s) 1667
Bst2UI CCWGG 4 cut(s) 287, 625, 807, 1412
Bst4CI ACNGT 3 cut(s) 56, 369, 941
Bst6I CTCTTC 1 cut(s) 564
BstACI GRCGYC 1 cut(s) 735
BstAPI GCANNNNNTGC 1 cut(s) 650
BstC8I GCNNGC 6 cut(s) 159, 319, 331, 361, 396, 856
BstDEI CTNAG 2 cut(s) 922, 1678
BstEII GGTNACC 1 cut(s) 1229
BstF5I GGATG 2 cut(s) 194, 619
BstFNI CGCG 2 cut(s) 701, 743
BstHHI GCGC 1 cut(s) 335
BstMAI GTCTC 2 cut(s) 836, 1685
BstMCI CGRYCG 1 cut(s) 395
BstMWI GCNNNNNNNGC 2 cut(s) 167, 650
BstNI CCWGG 4 cut(s) 287, 625, 807, 1412
BstPI GGTNACC 1 cut(s) 1229
BstSCI CCNGG 4 cut(s) 285, 623, 805, 1410
BstSFI CTRYAG 2 cut(s) 165, 1507
BstUI CGCG 2 cut(s) 701, 743
BstV1I GCAGC 5 cut(s) 148, 256, 385, 628, 1162
BstV2I GAAGAC 1 cut(s) 730
BstX2I RGATCY 3 cut(s) 665, 802, 1388
BstYI RGATCY 3 cut(s) 665, 802, 1388
BstZ17I GTATAC 1 cut(s) 937
BstZI CGGCCG 1 cut(s) 392
Bsu36I CCTNAGG 1 cut(s) 1678
BsuRI GGCC 4 cut(s) 285, 321, 363, 394
BtrI CACGTC 1 cut(s) 120
BtsCI GGATG 2 cut(s) 194, 619
BtsIMutI CAGTG 1 cut(s) 1405
Cac8I GCNNGC 6 cut(s) 159, 319, 331, 361, 396, 856
CfoI GCGC 1 cut(s) 335
Cfr10I RCCGGY 1 cut(s) 394
Cfr13I GGNCC 2 cut(s) 239, 319
CseI GACGC 1 cut(s) 724
Csp6I GTAC 1 cut(s) 112
CviAII CATG 4 cut(s) 86, 202, 916, 1252
CviQI GTAC 1 cut(s) 112
DdeI CTNAG 2 cut(s) 922, 1678
DraIII CACNNNGTG 1 cut(s) 1672
DrdI GACNNNNNNGTC 1 cut(s) 59
DseDI GACNNNNNNGTC 1 cut(s) 59
EaeI YGGCCR 1 cut(s) 392
EagI CGGCCG 1 cut(s) 392
Eam1104I CTCTTC 1 cut(s) 564
EarI CTCTTC 1 cut(s) 564
EclXI CGGCCG 1 cut(s) 392
Eco47I GGWCC 1 cut(s) 239
Eco52I CGGCCG 1 cut(s) 392
Eco81I CCTNAGG 1 cut(s) 1678
Eco88I CYCGRG 1 cut(s) 1235
Eco91I GGTNACC 1 cut(s) 1229
EcoO65I GGTNACC 1 cut(s) 1229
EcoRII CCWGG 4 cut(s) 285, 623, 805, 1410
FaeI CATG 4 cut(s) 89, 205, 919, 1255
FaqI GGGAC 1 cut(s) 1527
FatI CATG 4 cut(s) 85, 201, 915, 1251
FauI CCCGC 1 cut(s) 310
FbaI TGATCA 1 cut(s) 442
FblI GTMKAC 2 cut(s) 936, 988
Fnu4HI GCNGC 8 cut(s) 162, 270, 399, 639, 642, 1176, 1311, 1611
FokI GGATG 2 cut(s) 181, 606
Fsp4HI GCNGC 8 cut(s) 162, 270, 399, 639, 642, 1176, 1311, 1611
FspBI CTAG 6 cut(s) 20, 182, 911, 1088, 1377, 1517
GlaI GCGC 1 cut(s) 334
GluI GCNGC 8 cut(s) 162, 270, 399, 639, 642, 1176, 1311, 1611
GsuI CTGGAG 1 cut(s) 1099
HaeIII GGCC 4 cut(s) 285, 321, 363, 394
HapII CCGG 4 cut(s) 322, 395, 1640, 1697
HgaI GACGC 1 cut(s) 724
HhaI GCGC 1 cut(s) 335
Hin1I GRCGYC 1 cut(s) 735
Hin1II CATG 4 cut(s) 89, 205, 919, 1255
Hin6I GCGC 1 cut(s) 333
HinP1I GCGC 1 cut(s) 333
HincII GTYRAC 1 cut(s) 1122
HindII GTYRAC 1 cut(s) 1122
HindIII AAGCTT 1 cut(s) 856
HinfI GANTC 8 cut(s) 14, 45, 767, 985, 1103, 1192, 1489, 1693
HpaI GTTAAC 1 cut(s) 1122
HpaII CCGG 4 cut(s) 322, 395, 1640, 1697
HphI GGTGA 4 cut(s) 262, 828, 1141, 1241
Hpy166II GTNNAC 5 cut(s) 82, 937, 989, 1122, 1567
Hpy188I TCNGA 3 cut(s) 124, 340, 761
Hpy8I GTNNAC 5 cut(s) 82, 937, 989, 1122, 1567
Hpy99I CGWCG 1 cut(s) 422
HpyAV CCTTC 2 cut(s) 937, 976
HpyCH4III ACNGT 3 cut(s) 56, 369, 941
HpyCH4IV ACGT 7 cut(s) 110, 119, 586, 892, 1111, 1118, 1124
HpyCH4V TGCA 2 cut(s) 644, 794
HpyF10VI GCNNNNNNNGC 2 cut(s) 167, 650
HpyF3I CTNAG 2 cut(s) 922, 1678
HpySE526I ACGT 7 cut(s) 110, 119, 586, 892, 1111, 1118, 1124
Hsp92I GRCGYC 1 cut(s) 735
Hsp92II CATG 4 cut(s) 89, 205, 919, 1255
HspAI GCGC 1 cut(s) 333
Kpn2I TCCGGA 1 cut(s) 1696
KroI GCCGGC 1 cut(s) 394
KroNI GCCGGC 1 cut(s) 396
Ksp22I TGATCA 1 cut(s) 442
KspAI GTTAAC 1 cut(s) 1122
LmnI GCTCC 4 cut(s) 356, 615, 960, 1064
Lsp1109I GCAGC 5 cut(s) 148, 256, 385, 628, 1162
LweI GCATC 2 cut(s) 344, 781
MaeI CTAG 6 cut(s) 20, 182, 911, 1088, 1377, 1517
MaeII ACGT 7 cut(s) 110, 119, 586, 892, 1111, 1118, 1124
MaeIII GTNAC 2 cut(s) 1083, 1229
MflI RGATCY 3 cut(s) 665, 802, 1388
MhlI GDGCHC 1 cut(s) 965
MlyI GAGTC 4 cut(s) 8, 994, 1112, 1687
MroI TCCGGA 1 cut(s) 1696
MroNI GCCGGC 1 cut(s) 394
MseI TTAA 7 cut(s) 246, 309, 431, 1121, 1206, 1268, 1473
MslI CAYNNNNRTG 1 cut(s) 203
MspA1I CMGCKG 1 cut(s) 157
MspI CCGG 4 cut(s) 322, 395, 1640, 1697
MspR9I CCNGG 4 cut(s) 287, 625, 807, 1412
Mva1269I GAATGC 2 cut(s) 271, 1257
MvaI CCWGG 4 cut(s) 287, 625, 807, 1412
MvnI CGCG 2 cut(s) 701, 743
MwoI GCNNNNNNNGC 2 cut(s) 167, 650
NaeI GCCGGC 1 cut(s) 396
NgoMIV GCCGGC 1 cut(s) 394
NlaIII CATG 4 cut(s) 89, 205, 919, 1255
NlaIV GGNNCC 2 cut(s) 617, 1390
NmuCI GTSAC 1 cut(s) 1229
PcsI WCGNNNNNNNCGW 1 cut(s) 116
PctI GAATGC 2 cut(s) 271, 1257
PdiI GCCGGC 1 cut(s) 396
PfeI GAWTC 4 cut(s) 45, 767, 1192, 1489
PfoI TCCNGGA 1 cut(s) 805
PkrI GCNGC 8 cut(s) 163, 271, 400, 640, 643, 1177, 1312, 1612
PleI GAGTC 4 cut(s) 8, 993, 1111, 1687
PpsI GAGTC 4 cut(s) 8, 993, 1111, 1687
Psp1406I AACGTT 2 cut(s) 892, 1124
Psp6I CCWGG 4 cut(s) 285, 623, 805, 1410
PspEI GGTNACC 1 cut(s) 1229
PspGI CCWGG 4 cut(s) 285, 623, 805, 1410
PspN4I GGNNCC 2 cut(s) 617, 1390
PspPI GGNCC 2 cut(s) 239, 319
PsuI RGATCY 3 cut(s) 665, 802, 1388
PvuII CAGCTG 1 cut(s) 157
RsaI GTAC 1 cut(s) 113
RsaNI GTAC 1 cut(s) 112
RseI CAYNNNNRTG 1 cut(s) 203
SaqAI TTAA 7 cut(s) 246, 309, 431, 1121, 1206, 1268, 1473
SatI GCNGC 8 cut(s) 162, 270, 399, 639, 642, 1176, 1311, 1611
Sau96I GGNCC 2 cut(s) 239, 319
SchI GAGTC 4 cut(s) 8, 994, 1112, 1687
ScrFI CCNGG 4 cut(s) 287, 625, 807, 1412
SduI GDGCHC 1 cut(s) 965
SfaNI GCATC 2 cut(s) 344, 781
SfcI CTRYAG 2 cut(s) 165, 1507
SinI GGWCC 1 cut(s) 239
SmiMI CAYNNNNRTG 1 cut(s) 203
SmlI CTYRAG 1 cut(s) 64
SmoI CTYRAG 1 cut(s) 64
SpeI ACTAGT 2 cut(s) 1087, 1516
SsiI CCGC 9 cut(s) 317, 606, 638, 701, 1310, 1337, 1434, 1611, 1688
SspI AATATT 2 cut(s) 428, 1658
SspMI CTAG 6 cut(s) 20, 182, 911, 1088, 1377, 1517
StyD4I CCNGG 4 cut(s) 285, 623, 805, 1410
TaaI ACNGT 3 cut(s) 56, 369, 941
TaiI ACGT 7 cut(s) 113, 122, 589, 895, 1114, 1121, 1127
TaqI TCGA 8 cut(s) 33, 39, 417, 497, 530, 1326, 1342, 1718
TaqII GACCGA 1 cut(s) 227
TauI GCSGC 3 cut(s) 641, 1313, 1613
TfiI GAWTC 4 cut(s) 45, 767, 1192, 1489
Tru1I TTAA 7 cut(s) 246, 309, 431, 1121, 1206, 1268, 1473
Tru9I TTAA 7 cut(s) 246, 309, 431, 1121, 1206, 1268, 1473
TscAI CASTG 1 cut(s) 1405
TseFI GTSAC 1 cut(s) 1229
TseI GCWGC 5 cut(s) 161, 269, 398, 641, 1175
Tsp45I GTSAC 1 cut(s) 1229
TspDTI ATGAA 9 cut(s) 17, 211, 294, 619, 780, 919, 1513, 1638, 1727
TspGWI ACGGA 2 cut(s) 369, 1051
TspRI CASTG 1 cut(s) 1405
VpaK11BI GGWCC 1 cut(s) 239
XapI RAATTY 3 cut(s) 126, 410, 1245
XmiI GTMKAC 2 cut(s) 936, 988
XspI CTAG 6 cut(s) 20, 182, 911, 1088, 1377, 1517
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.