Rroxscaffold_6G00404870

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0000950
Physical Location & Seq
Reverse (-)
27358868 .. 27370019
11152 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00404870.1

Sequence Viewer

Length: 1578 bp
ATGCCCAGAATACTTGCAATAGTGAGAGACTGGTTTCAAAATTACCGCTACTTTTCCAGGGCTACAATATGCATCCAGGGTTTGGTTTTCAGCCATAGCTTCGCAACCCATGCCATAGTATCTATATTGATAACGTACCTTACGACTATGTGGAAGCATCCACATGCTCAGATAGCTGTTGTAGTGATGAATGTGCAAGATGGGGTATTAGACATTATGGTGATTATCCTCGCGCATATTCCTCGCACGTACATCGGTTTGTTTAGAGTAATTGCCACCACCAATGCTTCATATATTATTGGGCTTCTTCTGTTATGGCTTTCTGCTAAGTATATGAGCGAGGGTACTGCATCTAGGGTATTCTATGGAGCTATGGTACTAATAACATTAGGCGAAGCTGGGCGGTGGGCTGCTTTGCGAGAATTTCTGGATGAACAATATTCAAGAGAACAAAATCATGCGGAACGCACATATGATGAAAACCTGGATAAGAAAGATGCTTTGTGGATGCTTCCTTGGTTTATAGGTGCAGTGATTCCTCTTTTCCTTTTAAAAACAACTTGGCCACAAATATTCATGATATCAACAATTGCAATGGCGGTTTCTTATTTAGGGTTCTTGTTGGGCTTTCCTTATTATTTCACATACAATATGACTAATACTGAACAAGGTCAAGATGCTCGCCCAGAAGAAGTCAATCATCTAGAACAAGCACCAAGAGTCCCTGAACAAACAAGGTCAGGAAATGTATGGAGACTGATAAATATATTAAGGAAAGATGAACGTTTTCTCATAGAAGTATTAGCTATATGGTTAGCCTTCCTCCTTGCATACAGTGTTGTAAAGGCAGCAGGAAGCACGTTTTTTTTTCAACAAATGGATAACTTGGATAACCCTTTCGATAACTATGATCCTGCAGTCTATTTCAATGTGTTTGCCTCATTTTCAAAGTACTTGATCTCATTTCTCTGGAACTTAATTCCAAGGAAATGGAAACCATCGCGGCTGAGGATTTGGTGCGGAATGGCTTGTATTGTGCTTTGTTGTCTTGTTGCTTCGCAAGTTGAGAGCCACAGGTTAAACACTTCTTCAAATACGAGCATCTTTTGGTTGGTTCCTCAGTTCTTCTTACTAGGAGTCATGGAAGGCCTAGCCATGTATGGAATGATCGGTTTTCTTGTTGATCGAGTGTCTGAAGTTGATAAAGTAATGGTCGCATATTATGGATCCCACACCACTGGCTTGATCATAGGTATTGGAAAAATACTTGTTGGTCTCAGTGTTTTGGCGTTCAGAGGAAGCTGGTTTGATGACAACAACTTAGATGCCAGTCATCTTGATAAATATTACAGAGCATTAACAATCTTAACTTCTGTTGTCTTCTTCTTCCACTGTTGTATCGCCTTTTGCTATTACACTAGGGAAGAATTACAAGATGAGACGCCTTCATCGGTGACAATTGAGATGGTAAATAATCAGTCACAACCAGAGCTCAACCTGCCGCGGCATAATGTTGATAATGAATTTACCAAGATGCTTGTTGAGACAGTTGGAGGACAATCTCATACATATGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

525

Amino Acids

60.25

Weight (kDa)

6.24

Isoelectric Point (pI)

38.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1506
AccB7I CCANNNNNTGG 1 cut(s) 82
AccII CGCG 3 cut(s) 233, 1003, 1504
AciI CCGC 8 cut(s) 46, 403, 461, 599, 1003, 1020, 1502, 1504
AclI AACGTT 1 cut(s) 784
AclWI GGATC 3 cut(s) 905, 1221, 1234
AcoI YGGCCR 1 cut(s) 563
AcsI RAATTY 2 cut(s) 422, 1523
AcuI CTGAAG 1 cut(s) 1215
AcyI GRCGYC 1 cut(s) 1442
AfaI GTAC 5 cut(s) 137, 251, 346, 378, 953
AfiI CCNNNNNNNGG 1 cut(s) 82
AgsI TTSAA 6 cut(s) 38, 444, 872, 928, 948, 1092
AjnI CCWGG 3 cut(s) 56, 75, 483
AluBI AGCT 7 cut(s) 99, 176, 371, 398, 806, 1302, 1492
AluI AGCT 7 cut(s) 99, 176, 371, 398, 806, 1302, 1492
Alw21I GWGCWC 1 cut(s) 1494
Alw26I GTCTC 5 cut(s) 21, 748, 1280, 1433, 1538
AlwI GGATC 3 cut(s) 905, 1221, 1234
AoxI GGCC 2 cut(s) 563, 1147
ApeKI GCWGC 2 cut(s) 410, 848
ApoI RAATTY 2 cut(s) 422, 1523
Asp700I GAANNNNTTC 1 cut(s) 786
AspLEI GCGC 1 cut(s) 235
AsuHPI GGTGA 2 cut(s) 232, 1465
BaeI ACNNNNGTAYC 2 cut(s) 1381, 1414
BalI TGGCCA 1 cut(s) 565
BamHI GGATCC 1 cut(s) 1226
BanII GRGCYC 1 cut(s) 1494
BbsI GAAGAC 1 cut(s) 1372
Bbv12I GWGCWC 1 cut(s) 1494
BbvCI CCTCAGC 1 cut(s) 1007
BbvI GCAGC 2 cut(s) 397, 860
BccI CCATC 3 cut(s) 194, 1006, 1459
BcgI CGANNNNNNTGC 6 cut(s) 224, 235, 258, 269, 329, 363
BciT130I CCWGG 3 cut(s) 58, 77, 485
BclI TGATCA 1 cut(s) 1245
BcoDI GTCTC 5 cut(s) 21, 748, 1280, 1433, 1538
BfaI CTAG 5 cut(s) 354, 704, 1133, 1151, 1419
BfmI CTRYAG 1 cut(s) 915
BfuAI ACCTGC 1 cut(s) 1506
BisI GCNGC 5 cut(s) 411, 849, 1004, 1502, 1505
BlsI GCNGC 5 cut(s) 412, 850, 1005, 1503, 1506
BmcAI AGTACT 1 cut(s) 953
Bme1390I CCNGG 3 cut(s) 58, 77, 485
BmiI GGNNCC 2 cut(s) 1116, 1228
BmrFI CCNGG 3 cut(s) 58, 77, 485
BmsI GCATC 9 cut(s) 81, 166, 359, 487, 498, 667, 1110, 1315, 1524
BpiI GAAGAC 1 cut(s) 1372
Bpu10I CCTNAGC 1 cut(s) 1007
BsaAI YACGTR 1 cut(s) 249
BsaHI GRCGYC 1 cut(s) 1442
BsaI GGTCTC 1 cut(s) 1280
BsaJI CCNNGG 5 cut(s) 57, 76, 515, 983, 1502
BsaXI ACNNNNNCTCC 2 cut(s) 360, 390
Bsc4I CCNNNNNNNGG 1 cut(s) 82
Bse1I ACTGG 3 cut(s) 35, 1243, 1329
Bse3DI GCAATG 1 cut(s) 600
BseBI CCWGG 3 cut(s) 58, 77, 485
BseDI CCNNGG 5 cut(s) 57, 76, 515, 983, 1502
BseGI GGATG 4 cut(s) 72, 157, 436, 513
BseLI CCNNNNNNNGG 1 cut(s) 82
BseMI GCAATG 1 cut(s) 600
BseMII CTCAG 4 cut(s) 182, 998, 1133, 1291
BseNI ACTGG 3 cut(s) 35, 1243, 1329
BseXI GCAGC 2 cut(s) 397, 860
BseYI CCCAGC 1 cut(s) 398
BsgI GTGCAG 1 cut(s) 549
Bsh1236I CGCG 3 cut(s) 233, 1003, 1504
BshFI GGCC 2 cut(s) 565, 1149
BsiHKAI GWGCWC 1 cut(s) 1494
BslFI GGGAC 1 cut(s) 707
BslI CCNNNNNNNGG 1 cut(s) 82
BsmAI GTCTC 5 cut(s) 21, 748, 1280, 1433, 1538
BsmBI CGTCTC 1 cut(s) 1433
BsmFI GGGAC 1 cut(s) 707
BsnI GGCC 2 cut(s) 565, 1149
Bso31I GGTCTC 1 cut(s) 1280
Bsp1286I GDGCHC 1 cut(s) 1494
Bsp143I GATC 6 cut(s) 910, 957, 1167, 1183, 1226, 1245
BspACI CCGC 8 cut(s) 46, 403, 461, 599, 1003, 1020, 1502, 1504
BspANI GGCC 2 cut(s) 565, 1149
BspCNI CTCAG 4 cut(s) 181, 999, 1132, 1290
BspFNI CGCG 3 cut(s) 233, 1003, 1504
BspHI TCATGA 1 cut(s) 576
BspLI GGNNCC 2 cut(s) 1116, 1228
BspMAI CTGCAG 1 cut(s) 919
BspMI ACCTGC 1 cut(s) 1506
BspPI GGATC 3 cut(s) 905, 1221, 1234
BspTNI GGTCTC 1 cut(s) 1280
BsrDI GCAATG 1 cut(s) 600
BsrI ACTGG 3 cut(s) 35, 1243, 1329
BssECI CCNNGG 5 cut(s) 57, 76, 515, 983, 1502
BssMI GATC 6 cut(s) 910, 957, 1167, 1183, 1226, 1245
BssNI GRCGYC 1 cut(s) 1442
BssT1I CCWWGG 2 cut(s) 515, 983
Bst2UI CCWGG 3 cut(s) 58, 77, 485
Bst4CI ACNGT 3 cut(s) 836, 1394, 1549
BstACI GRCGYC 1 cut(s) 1442
BstAPI GCANNNNNTGC 1 cut(s) 110
BstBAI YACGTR 1 cut(s) 249
BstC8I GCNNGC 1 cut(s) 682
BstDEI CTNAG 6 cut(s) 168, 327, 1007, 1119, 1277, 1321
BstDSI CCRYGG 1 cut(s) 1502
BstF5I GGATG 4 cut(s) 72, 157, 436, 513
BstFNI CGCG 3 cut(s) 233, 1003, 1504
BstHHI GCGC 1 cut(s) 235
BstKTI GATC 6 cut(s) 913, 960, 1170, 1186, 1229, 1248
BstMAI GTCTC 5 cut(s) 21, 748, 1280, 1433, 1538
BstMBI GATC 6 cut(s) 910, 957, 1167, 1183, 1226, 1245
BstMWI GCNNNNNNNGC 3 cut(s) 110, 173, 1498
BstNI CCWGG 3 cut(s) 58, 77, 485
BstNSI RCATGY 1 cut(s) 167
BstSCI CCNGG 3 cut(s) 56, 75, 483
BstSFI CTRYAG 1 cut(s) 915
BstUI CGCG 3 cut(s) 233, 1003, 1504
BstV1I GCAGC 2 cut(s) 397, 860
BstV2I GAAGAC 1 cut(s) 1372
BstX2I RGATCY 1 cut(s) 1226
BstXI CCANNNNNNTGG 2 cut(s) 990, 1238
BstYI RGATCY 1 cut(s) 1226
BsuRI GGCC 2 cut(s) 565, 1149
BtgI CCRYGG 1 cut(s) 1502
BtgZI GCGATG 1 cut(s) 984
BtsCI GGATG 4 cut(s) 72, 157, 436, 513
BtsI GCAGTG 1 cut(s) 537
BtsIMutI CAGTG 5 cut(s) 537, 841, 1236, 1285, 1390
BveI ACCTGC 1 cut(s) 1506
Cac8I GCNNGC 1 cut(s) 682
CciI TCATGA 1 cut(s) 576
CfoI GCGC 1 cut(s) 235
Cfr42I CCGCGG 1 cut(s) 1505
CseI GACGC 1 cut(s) 1450
Csp6I GTAC 5 cut(s) 136, 250, 345, 377, 952
CviAII CATG 6 cut(s) 110, 164, 458, 577, 1141, 1156
CviQI GTAC 5 cut(s) 136, 250, 345, 377, 952
DdeI CTNAG 6 cut(s) 168, 327, 1007, 1119, 1277, 1321
DpnI GATC 6 cut(s) 912, 959, 1169, 1185, 1228, 1247
DpnII GATC 6 cut(s) 910, 957, 1167, 1183, 1226, 1245
DraI TTTAAA 1 cut(s) 552
EaeI YGGCCR 1 cut(s) 563
Ecl136II GAGCTC 1 cut(s) 1492
Eco130I CCWWGG 2 cut(s) 515, 983
Eco147I AGGCCT 1 cut(s) 1149
Eco24I GRGCYC 1 cut(s) 1494
Eco31I GGTCTC 1 cut(s) 1280
Eco32I GATATC 1 cut(s) 582
Eco53kI GAGCTC 1 cut(s) 1492
Eco57I CTGAAG 1 cut(s) 1215
EcoICRI GAGCTC 1 cut(s) 1492
EcoRII CCWGG 3 cut(s) 56, 75, 483
EcoRV GATATC 1 cut(s) 582
EcoT14I CCWWGG 2 cut(s) 515, 983
EcoT22I ATGCAT 1 cut(s) 74
EcoT38I GRGCYC 1 cut(s) 1494
ErhI CCWWGG 2 cut(s) 515, 983
Esp3I CGTCTC 1 cut(s) 1433
FaeI CATG 6 cut(s) 113, 167, 461, 580, 1144, 1159
FaqI GGGAC 1 cut(s) 707
FatI CATG 6 cut(s) 109, 163, 457, 576, 1140, 1155
FauNDI CATATG 2 cut(s) 472, 1570
FbaI TGATCA 1 cut(s) 1245
Fnu4HI GCNGC 5 cut(s) 411, 849, 1004, 1502, 1505
FokI GGATG 4 cut(s) 59, 144, 443, 520
FriOI GRGCYC 1 cut(s) 1494
Fsp4HI GCNGC 5 cut(s) 411, 849, 1004, 1502, 1505
FspBI CTAG 5 cut(s) 354, 704, 1133, 1151, 1419
GlaI GCGC 1 cut(s) 234
GluI GCNGC 5 cut(s) 411, 849, 1004, 1502, 1505
GsaI CCCAGC 1 cut(s) 402
HaeIII GGCC 2 cut(s) 565, 1149
HgaI GACGC 1 cut(s) 1450
HhaI GCGC 1 cut(s) 235
Hin1I GRCGYC 1 cut(s) 1442
Hin1II CATG 6 cut(s) 113, 167, 461, 580, 1144, 1159
Hin6I GCGC 1 cut(s) 233
HinP1I GCGC 1 cut(s) 233
HinfI GANTC 3 cut(s) 535, 720, 1137
HphI GGTGA 2 cut(s) 232, 1465
Hpy188I TCNGA 3 cut(s) 171, 1195, 1295
Hpy188III TCNNGA 8 cut(s) 428, 444, 577, 674, 704, 741, 970, 1337
HpyAV CCTTC 3 cut(s) 829, 1139, 1455
HpyCH4III ACNGT 3 cut(s) 836, 1394, 1549
HpyCH4IV ACGT 4 cut(s) 134, 248, 784, 860
HpyCH4V TGCA 8 cut(s) 17, 72, 196, 350, 530, 593, 830, 917
HpyF10VI GCNNNNNNNGC 3 cut(s) 110, 173, 1498
HpyF3I CTNAG 6 cut(s) 168, 327, 1007, 1119, 1277, 1321
HpySE526I ACGT 4 cut(s) 134, 248, 784, 860
Hsp92I GRCGYC 1 cut(s) 1442
Hsp92II CATG 6 cut(s) 113, 167, 461, 580, 1144, 1159
HspAI GCGC 1 cut(s) 233
Ksp22I TGATCA 1 cut(s) 1245
KspI CCGCGG 1 cut(s) 1505
Kzo9I GATC 6 cut(s) 910, 957, 1167, 1183, 1226, 1245
LmnI GCTCC 1 cut(s) 368
Lsp1109I GCAGC 2 cut(s) 397, 860
LweI GCATC 9 cut(s) 81, 166, 359, 487, 498, 667, 1110, 1315, 1524
MaeI CTAG 5 cut(s) 354, 704, 1133, 1151, 1419
MaeII ACGT 4 cut(s) 134, 248, 784, 860
MaeIII GTNAC 2 cut(s) 1453, 1479
MalI GATC 6 cut(s) 912, 959, 1169, 1185, 1228, 1247
MboI GATC 6 cut(s) 910, 957, 1167, 1183, 1226, 1245
MboII GAAGA 8 cut(s) 299, 701, 1080, 1117, 1372, 1375, 1378, 1436
MfeI CAATTG 2 cut(s) 588, 1458
MflI RGATCY 1 cut(s) 1226
MhlI GDGCHC 1 cut(s) 1494
MlsI TGGCCA 1 cut(s) 565
MluCI AATT 8 cut(s) 40, 270, 422, 588, 978, 1427, 1458, 1523
MluNI TGGCCA 1 cut(s) 565
MlyI GAGTC 2 cut(s) 729, 1146
MmeI TCCRAC 1 cut(s) 1531
Mox20I TGGCCA 1 cut(s) 565
Mph1103I ATGCAT 1 cut(s) 74
MroXI GAANNNNTTC 1 cut(s) 786
MscI TGGCCA 1 cut(s) 565
MseI TTAA 6 cut(s) 551, 770, 977, 1079, 1358, 1367
MslI CAYNNNNRTG 3 cut(s) 162, 218, 1569
Msp20I TGGCCA 1 cut(s) 565
MspA1I CMGCKG 1 cut(s) 1504
MspR9I CCNGG 3 cut(s) 58, 77, 485
MunI CAATTG 2 cut(s) 588, 1458
MvaI CCWGG 3 cut(s) 58, 77, 485
MvnI CGCG 3 cut(s) 233, 1003, 1504
MwoI GCNNNNNNNGC 3 cut(s) 110, 173, 1498
NdeI CATATG 2 cut(s) 472, 1570
NdeII GATC 6 cut(s) 910, 957, 1167, 1183, 1226, 1245
NlaIII CATG 6 cut(s) 113, 167, 461, 580, 1144, 1159
NlaIV GGNNCC 2 cut(s) 1116, 1228
NmuCI GTSAC 2 cut(s) 1453, 1479
NsiI ATGCAT 1 cut(s) 74
NspI RCATGY 1 cut(s) 167
PagI TCATGA 1 cut(s) 576
PceI AGGCCT 1 cut(s) 1149
PcsI WCGNNNNNNNCGW 1 cut(s) 140
PdmI GAANNNNTTC 1 cut(s) 786
PfeI GAWTC 1 cut(s) 535
PflMI CCANNNNNTGG 1 cut(s) 82
PkrI GCNGC 5 cut(s) 412, 850, 1005, 1503, 1506
PleI GAGTC 2 cut(s) 728, 1145
PpsI GAGTC 2 cut(s) 728, 1145
Ppu21I YACGTR 1 cut(s) 249
Psp124BI GAGCTC 1 cut(s) 1494
Psp1406I AACGTT 1 cut(s) 784
Psp6I CCWGG 3 cut(s) 56, 75, 483
PspFI CCCAGC 1 cut(s) 398
PspGI CCWGG 3 cut(s) 56, 75, 483
PspN4I GGNNCC 2 cut(s) 1116, 1228
PstI CTGCAG 1 cut(s) 919
PsuI RGATCY 1 cut(s) 1226
RsaI GTAC 5 cut(s) 137, 251, 346, 378, 953
RsaNI GTAC 5 cut(s) 136, 250, 345, 377, 952
RseI CAYNNNNRTG 3 cut(s) 162, 218, 1569
SacI GAGCTC 1 cut(s) 1494
SacII CCGCGG 1 cut(s) 1505
SaqAI TTAA 6 cut(s) 551, 770, 977, 1079, 1358, 1367
SatI GCNGC 5 cut(s) 411, 849, 1004, 1502, 1505
Sau3AI GATC 6 cut(s) 910, 957, 1167, 1183, 1226, 1245
ScaI AGTACT 1 cut(s) 953
SchI GAGTC 2 cut(s) 729, 1146
ScrFI CCNGG 3 cut(s) 58, 77, 485
SduI GDGCHC 1 cut(s) 1494
SfaNI GCATC 9 cut(s) 81, 166, 359, 487, 498, 667, 1110, 1315, 1524
SfcI CTRYAG 1 cut(s) 915
Sfr303I CCGCGG 1 cut(s) 1505
SgrBI CCGCGG 1 cut(s) 1505
SmiMI CAYNNNNRTG 3 cut(s) 162, 218, 1569
Sse9I AATT 8 cut(s) 40, 270, 422, 588, 978, 1427, 1458, 1523
SseBI AGGCCT 1 cut(s) 1149
SsiI CCGC 8 cut(s) 46, 403, 461, 599, 1003, 1020, 1502, 1504
SspI AATATT 3 cut(s) 440, 573, 1346
SspMI CTAG 5 cut(s) 354, 704, 1133, 1151, 1419
SstI GAGCTC 1 cut(s) 1494
StuI AGGCCT 1 cut(s) 1149
StyD4I CCNGG 3 cut(s) 56, 75, 483
StyI CCWWGG 2 cut(s) 515, 983
TaaI ACNGT 3 cut(s) 836, 1394, 1549
TaiI ACGT 4 cut(s) 137, 251, 787, 863
TaqI TCGA 2 cut(s) 900, 1186
TasI AATT 8 cut(s) 40, 270, 422, 588, 978, 1427, 1458, 1523
TatI WGTACW 1 cut(s) 951
TauI GCSGC 3 cut(s) 1006, 1504, 1507
TfiI GAWTC 1 cut(s) 535
Tru1I TTAA 6 cut(s) 551, 770, 977, 1079, 1358, 1367
Tru9I TTAA 6 cut(s) 551, 770, 977, 1079, 1358, 1367
TscAI CASTG 5 cut(s) 537, 841, 1243, 1285, 1397
TseFI GTSAC 2 cut(s) 1453, 1479
TseI GCWGC 2 cut(s) 410, 848
Tsp45I GTSAC 2 cut(s) 1453, 1479
TspDTI ATGAA 8 cut(s) 203, 279, 447, 492, 565, 795, 1437, 1536
TspRI CASTG 5 cut(s) 537, 841, 1243, 1285, 1397
Van91I CCANNNNNTGG 1 cut(s) 82
XapI RAATTY 2 cut(s) 422, 1523
XbaI TCTAGA 1 cut(s) 703
XceI RCATGY 1 cut(s) 167
XmnI GAANNNNTTC 1 cut(s) 786
XspI CTAG 5 cut(s) 354, 704, 1133, 1151, 1419
ZrmI AGTACT 1 cut(s) 953
Zsp2I ATGCAT 1 cut(s) 74
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.