Rroxscaffold_5G00365230

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000950
Physical Location & Seq
Forward (+)
46693809 .. 46697513
3705 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00365230.1

Sequence Viewer

Length: 1440 bp
ATGAGGAGTAGAAGGAAAATTGCTCTAACAATGGGACCAATAAGGAATCTACAATTAAGTCATAACTTTGTAGGCCGTGCGGTATCGTCCATCTTGATCACGTACCTCACGCTTCATATTATGAAGAACTATCAAATGGCCGCTGTACTTATAAATGTGGTATACGGCGCAGCATGTATCATGGTCATCGTCCTTGCTTATCTATCACACAAAATAGGTCATTTCAGAATGGTTGTCTTCACAAGTTTTGCCTATAGTCTTGGACTGGCTCTATTATGGATTTCAGCCAAGTTTATACGTCCGGGCGCAGATGCAGCAGATGATTTTTTCTATTGGGCTGCAGTACTATTGGCAATAGGTGAAGCTGGAAGGCCTGCGGCTATACTTAAATTTCTTGACGATCAATATATAAAAGAACAGAGACAAAGAGATCATGAGTACACAAAAAAAGACGAAGAACGATTTCAAAGCCGCAAAGATGCTTTGTGGAGCCATCCCTGGTTTCTCGGTGCAGTGGCAGCTCTTTTCATTCCTGGAGAGGCGGGTTGGGAAACTGTCTTCCTGATCTCCATGATTGCAATGGGGGTTTGTTTATTATTGTTCTGGTTTGGAAAAACCTTTTATTCAGTCTCATCTTCAGAAGAAACAGCACCAAAAGGGAAGTTTGCGGTCACACCGAAAGAAAATGGACACCGTTTTGCAGAAATGTTAGCTATGATCTTGGCCTTCGTTGCATACAGTCTGGTGGAGGCAACGGGATCCACGTTCTTTTTTGAACAAACAACTTACTTGGACAATCATGTAGGAAATTTCACAGTTCCTGTCGTATATCTAACTGTACTGCAGTCCTTTTCAAAGTACATAATCCCGCATCTTTGGGGGCTACTAATTCCAAAGCAGCGAAGAAGTGTTGCATTGGTTAAAATTGGGTGCGGTTTGGTTCTTTCTGTTTTATGCTGTGTCATTGCTTGGCGAATTGAGATTCGTAGGGCAAGTAAAATCAAGGTGGAAGTTGACCCTGAAAAAGATAACAAACCAATTATTTCCATGAGCATCCTTTGGTTACTTCCACAATTCTCTCTGCTAGGGCTCATGAGAGGGTTAGCCGAAGCCGGATTTGACGAGTTTTTTGCGGAACAAGTAGTTGACGAAGATAAAGAAGTGGTGCTTAGGTATTATGGACCTCACGCATTTGACTTCGTCCTGGGTATTGGAACGTTACTGACCGCTGTTAGTATTTTGGGGTTTAGACGAACTTGGTTGGATAATAATATATATAAGAATCGTCTAGATAAGTATTACAGGGGATTAGTATTCCTAGGTGTTGCTAACTTTGGCTACTACTTGTGTGTCGCCCTTTACTTTTACACCAAGCATGAGTCAGAAATTTCTGCCGAAGAAACAAATGAGGAAGAAACTGCAAATAAAGGGGAGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

479

Amino Acids

54.34

Weight (kDa)

8.34

Isoelectric Point (pI)

37.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 24 - 324 3.1e-07 Major Facilitator Superfamily
PTR2 PF00854 75 - 219 5.4e-07 POT family
PTR2 PF00854 227 - 389 1.5e-10 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 152
AccI GTMKAC 1 cut(s) 162
AclI AACGTT 1 cut(s) 1217
AclWI GGATC 2 cut(s) 753, 766
AcoI YGGCCR 1 cut(s) 138
AcsI RAATTY 3 cut(s) 389, 808, 1386
AcuI CTGAAG 1 cut(s) 621
AfaI GTAC 6 cut(s) 104, 147, 345, 440, 840, 860
AgsI TTSAA 3 cut(s) 467, 776, 855
AjnI CCWGG 3 cut(s) 497, 532, 1203
AluBI AGCT 3 cut(s) 365, 521, 713
AluI AGCT 3 cut(s) 365, 521, 713
Alw26I GTCTC 2 cut(s) 415, 634
AlwI GGATC 2 cut(s) 753, 766
AlwNI CAGNNNCTG 1 cut(s) 821
AoxI GGCC 4 cut(s) 73, 138, 371, 723
ApeKI GCWGC 5 cut(s) 170, 314, 338, 518, 898
ApoI RAATTY 3 cut(s) 389, 808, 1386
ArsI GACNNNNNNTTYG 2 cut(s) 1112, 1144
Asp700I GAANNNNTTC 1 cut(s) 462
AspA2I CCTAGG 1 cut(s) 1318
AspLEI GCGC 2 cut(s) 170, 308
AspS9I GGNCC 2 cut(s) 35, 1181
AsuC2I CCSGG 1 cut(s) 303
AsuHPI GGTGA 1 cut(s) 371
AvaII GGWCC 2 cut(s) 35, 1181
AvrII CCTAGG 1 cut(s) 1318
BamHI GGATCC 1 cut(s) 758
BanII GRGCYC 1 cut(s) 1092
BbsI GAAGAC 2 cut(s) 229, 550
BbvI GCAGC 5 cut(s) 182, 325, 326, 530, 910
BccI CCATC 2 cut(s) 98, 501
BceAI ACGGC 2 cut(s) 60, 181
BcgI CGANNNNNNTGC 2 cut(s) 1112, 1146
BciT130I CCWGG 3 cut(s) 499, 534, 1205
BclI TGATCA 1 cut(s) 96
BcnI CCSGG 1 cut(s) 303
BcoDI GTCTC 2 cut(s) 415, 634
BfaI CTAG 3 cut(s) 1085, 1289, 1319
BfmI CTRYAG 3 cut(s) 253, 339, 842
BisI GCNGC 8 cut(s) 141, 171, 315, 339, 378, 472, 519, 899
BlnI CCTAGG 1 cut(s) 1318
BlsI GCNGC 8 cut(s) 142, 172, 316, 340, 379, 473, 520, 900
BmcAI AGTACT 1 cut(s) 345
Bme1390I CCNGG 4 cut(s) 303, 499, 534, 1205
Bme18I GGWCC 2 cut(s) 35, 1181
BmgT120I GGNCC 2 cut(s) 35, 1181
BmiI GGNNCC 3 cut(s) 36, 491, 760
BmrFI CCNGG 4 cut(s) 303, 499, 534, 1205
BmsI GCATC 4 cut(s) 301, 469, 880, 1062
BpiI GAAGAC 2 cut(s) 229, 550
BpmI CTGGAG 1 cut(s) 555
Bpu10I CCTNAGC 1 cut(s) 1169
BpuMI CCSGG 1 cut(s) 303
BsaAI YACGTR 1 cut(s) 102
BsaJI CCNNGG 3 cut(s) 497, 1204, 1318
Bse1I ACTGG 1 cut(s) 270
Bse3DI GCAATG 2 cut(s) 585, 963
BseBI CCWGG 3 cut(s) 499, 534, 1205
BseDI CCNNGG 3 cut(s) 497, 1204, 1318
BseGI GGATG 2 cut(s) 493, 1053
BseMI GCAATG 2 cut(s) 585, 963
BseNI ACTGG 1 cut(s) 270
BseRI GAGGAG 1 cut(s) 19
BseXI GCAGC 5 cut(s) 182, 325, 326, 530, 910
BsgI GTGCAG 1 cut(s) 531
BshFI GGCC 4 cut(s) 75, 140, 373, 725
BsiSI CCGG 2 cut(s) 302, 1113
BslFI GGGAC 1 cut(s) 48
BsmAI GTCTC 2 cut(s) 415, 634
BsmFI GGGAC 1 cut(s) 48
BsnI GGCC 4 cut(s) 75, 140, 373, 725
Bsp1286I GDGCHC 1 cut(s) 1092
Bsp143I GATC 6 cut(s) 96, 400, 430, 564, 717, 758
BspANI GGCC 4 cut(s) 75, 140, 373, 725
BspHI TCATGA 2 cut(s) 433, 1092
BspLI GGNNCC 3 cut(s) 36, 491, 760
BspMAI CTGCAG 2 cut(s) 343, 846
BspPI GGATC 2 cut(s) 753, 766
BsrDI GCAATG 2 cut(s) 585, 963
BsrI ACTGG 1 cut(s) 270
BssECI CCNNGG 3 cut(s) 497, 1204, 1318
BssMI GATC 6 cut(s) 96, 400, 430, 564, 717, 758
BssNAI GTATAC 1 cut(s) 163
BssT1I CCWWGG 1 cut(s) 1318
Bst1107I GTATAC 1 cut(s) 163
Bst2UI CCWGG 3 cut(s) 499, 534, 1205
Bst4CI ACNGT 5 cut(s) 556, 695, 740, 817, 838
BstBAI YACGTR 1 cut(s) 102
BstC8I GCNNGC 1 cut(s) 375
BstDEI CTNAG 1 cut(s) 1169
BstF5I GGATG 2 cut(s) 493, 1053
BstHHI GCGC 2 cut(s) 170, 308
BstKTI GATC 6 cut(s) 99, 403, 433, 567, 720, 761
BstMAI GTCTC 2 cut(s) 415, 634
BstMBI GATC 6 cut(s) 96, 400, 430, 564, 717, 758
BstMWI GCNNNNNNNGC 3 cut(s) 314, 518, 731
BstNI CCWGG 3 cut(s) 499, 534, 1205
BstNSI RCATGY 1 cut(s) 177
BstSCI CCNGG 4 cut(s) 301, 497, 532, 1203
BstSFI CTRYAG 3 cut(s) 253, 339, 842
BstV1I GCAGC 5 cut(s) 182, 325, 326, 530, 910
BstV2I GAAGAC 2 cut(s) 229, 550
BstX2I RGATCY 1 cut(s) 758
BstYI RGATCY 1 cut(s) 758
BstZ17I GTATAC 1 cut(s) 163
BsuRI GGCC 4 cut(s) 75, 140, 373, 725
BtsCI GGATG 2 cut(s) 493, 1053
BtsI GCAGTG 1 cut(s) 519
BtsIMutI CAGTG 1 cut(s) 519
Cac8I GCNNGC 1 cut(s) 375
CaiI CAGNNNCTG 1 cut(s) 821
CciI TCATGA 2 cut(s) 433, 1092
CfoI GCGC 2 cut(s) 170, 308
Cfr13I GGNCC 2 cut(s) 35, 1181
Csp6I GTAC 6 cut(s) 103, 146, 344, 439, 839, 859
CviAII CATG 8 cut(s) 174, 181, 434, 571, 800, 1048, 1093, 1376
CviQI GTAC 6 cut(s) 103, 146, 344, 439, 839, 859
DdeI CTNAG 1 cut(s) 1169
DpnI GATC 6 cut(s) 98, 402, 432, 566, 719, 760
DpnII GATC 6 cut(s) 96, 400, 430, 564, 717, 758
EaeI YGGCCR 1 cut(s) 138
Eco130I CCWWGG 1 cut(s) 1318
Eco147I AGGCCT 1 cut(s) 373
Eco24I GRGCYC 1 cut(s) 1092
Eco47I GGWCC 2 cut(s) 35, 1181
Eco57I CTGAAG 1 cut(s) 621
EcoRII CCWGG 3 cut(s) 497, 532, 1203
EcoT14I CCWWGG 1 cut(s) 1318
EcoT38I GRGCYC 1 cut(s) 1092
ErhI CCWWGG 1 cut(s) 1318
FaeI CATG 8 cut(s) 177, 184, 437, 574, 803, 1051, 1096, 1379
FalI AAGNNNNNCTT 2 cut(s) 1152, 1184
FaqI GGGAC 1 cut(s) 48
FatI CATG 8 cut(s) 173, 180, 433, 570, 799, 1047, 1092, 1375
FauI CCCGC 2 cut(s) 535, 876
FbaI TGATCA 1 cut(s) 96
FblI GTMKAC 1 cut(s) 162
Fnu4HI GCNGC 8 cut(s) 141, 171, 315, 339, 378, 472, 519, 899
FokI GGATG 2 cut(s) 480, 1040
FriOI GRGCYC 1 cut(s) 1092
Fsp4HI GCNGC 8 cut(s) 141, 171, 315, 339, 378, 472, 519, 899
FspBI CTAG 3 cut(s) 1085, 1289, 1319
GlaI GCGC 2 cut(s) 169, 307
GluI GCNGC 8 cut(s) 141, 171, 315, 339, 378, 472, 519, 899
GsuI CTGGAG 1 cut(s) 555
HaeIII GGCC 4 cut(s) 75, 140, 373, 725
HapII CCGG 2 cut(s) 302, 1113
HhaI GCGC 2 cut(s) 170, 308
Hin1II CATG 8 cut(s) 177, 184, 437, 574, 803, 1051, 1096, 1379
Hin6I GCGC 2 cut(s) 168, 306
HinP1I GCGC 2 cut(s) 168, 306
HincII GTYRAC 2 cut(s) 1015, 1147
HindII GTYRAC 2 cut(s) 1015, 1147
HinfI GANTC 4 cut(s) 46, 982, 1282, 1379
HpaII CCGG 2 cut(s) 302, 1113
HphI GGTGA 1 cut(s) 371
Hpy166II GTNNAC 4 cut(s) 163, 441, 1015, 1147
Hpy188I TCNGA 3 cut(s) 227, 640, 1384
Hpy188III TCNNGA 6 cut(s) 94, 395, 434, 562, 1093, 1289
Hpy8I GTNNAC 4 cut(s) 163, 441, 1015, 1147
HpyAV CCTTC 3 cut(s) 6, 363, 736
HpyCH4III ACNGT 5 cut(s) 556, 695, 740, 817, 838
HpyCH4IV ACGT 4 cut(s) 101, 298, 764, 1217
HpyCH4V TGCA 9 cut(s) 314, 341, 512, 578, 701, 734, 844, 914, 1421
HpyF10VI GCNNNNNNNGC 3 cut(s) 314, 518, 731
HpyF3I CTNAG 1 cut(s) 1169
HpySE526I ACGT 4 cut(s) 101, 298, 764, 1217
Hsp92II CATG 8 cut(s) 177, 184, 437, 574, 803, 1051, 1096, 1379
HspAI GCGC 2 cut(s) 168, 306
Ksp22I TGATCA 1 cut(s) 96
Kzo9I GATC 6 cut(s) 96, 400, 430, 564, 717, 758
LmnI GCTCC 1 cut(s) 489
Lsp1109I GCAGC 5 cut(s) 182, 325, 326, 530, 910
LweI GCATC 4 cut(s) 301, 469, 880, 1062
MaeI CTAG 3 cut(s) 1085, 1289, 1319
MaeII ACGT 4 cut(s) 101, 298, 764, 1217
MaeIII GTNAC 3 cut(s) 670, 1062, 1218
MalI GATC 6 cut(s) 98, 402, 432, 566, 719, 760
MboI GATC 6 cut(s) 96, 400, 430, 564, 717, 758
MflI RGATCY 1 cut(s) 758
MhlI GDGCHC 1 cut(s) 1092
MlyI GAGTC 1 cut(s) 1388
MmeI TCCRAC 1 cut(s) 1242
MnlI CCTC 6 cut(s) 116, 532, 742, 1091, 1194, 1402
MroXI GAANNNNTTC 1 cut(s) 462
MseI TTAA 3 cut(s) 56, 387, 921
MspA1I CMGCKG 2 cut(s) 143, 1229
MspI CCGG 2 cut(s) 302, 1113
MspR9I CCNGG 4 cut(s) 303, 499, 534, 1205
MvaI CCWGG 3 cut(s) 499, 534, 1205
MwoI GCNNNNNNNGC 3 cut(s) 314, 518, 731
NciI CCSGG 1 cut(s) 303
NdeII GATC 6 cut(s) 96, 400, 430, 564, 717, 758
NlaIII CATG 8 cut(s) 177, 184, 437, 574, 803, 1051, 1096, 1379
NlaIV GGNNCC 3 cut(s) 36, 491, 760
NmuCI GTSAC 1 cut(s) 670
NspI RCATGY 1 cut(s) 177
PagI TCATGA 2 cut(s) 433, 1092
PceI AGGCCT 1 cut(s) 373
PcsI WCGNNNNNNNCGW 1 cut(s) 761
PdmI GAANNNNTTC 1 cut(s) 462
PfeI GAWTC 3 cut(s) 46, 982, 1282
PflFI GACNNNGTC 1 cut(s) 1199
PfoI TCCNGGA 1 cut(s) 532
PkrI GCNGC 8 cut(s) 142, 172, 316, 340, 379, 473, 520, 900
PleI GAGTC 1 cut(s) 1387
PpsI GAGTC 1 cut(s) 1387
Ppu21I YACGTR 1 cut(s) 102
PsiI TTATAA 1 cut(s) 152
Psp1406I AACGTT 1 cut(s) 1217
Psp6I CCWGG 3 cut(s) 497, 532, 1203
PspGI CCWGG 3 cut(s) 497, 532, 1203
PspN4I GGNNCC 3 cut(s) 36, 491, 760
PspPI GGNCC 2 cut(s) 35, 1181
PstI CTGCAG 2 cut(s) 343, 846
PstNI CAGNNNCTG 1 cut(s) 821
PsuI RGATCY 1 cut(s) 758
PsyI GACNNNGTC 1 cut(s) 1199
RsaI GTAC 6 cut(s) 104, 147, 345, 440, 840, 860
RsaNI GTAC 6 cut(s) 103, 146, 344, 439, 839, 859
SaqAI TTAA 3 cut(s) 56, 387, 921
SatI GCNGC 8 cut(s) 141, 171, 315, 339, 378, 472, 519, 899
Sau3AI GATC 6 cut(s) 96, 400, 430, 564, 717, 758
Sau96I GGNCC 2 cut(s) 35, 1181
ScaI AGTACT 1 cut(s) 345
SchI GAGTC 1 cut(s) 1388
ScrFI CCNGG 4 cut(s) 303, 499, 534, 1205
SduI GDGCHC 1 cut(s) 1092
SfaNI GCATC 4 cut(s) 301, 469, 880, 1062
SfcI CTRYAG 3 cut(s) 253, 339, 842
SinI GGWCC 2 cut(s) 35, 1181
SseBI AGGCCT 1 cut(s) 373
SspMI CTAG 3 cut(s) 1085, 1289, 1319
StuI AGGCCT 1 cut(s) 373
StyD4I CCNGG 4 cut(s) 301, 497, 532, 1203
StyI CCWWGG 1 cut(s) 1318
TaaI ACNGT 5 cut(s) 556, 695, 740, 817, 838
TaiI ACGT 4 cut(s) 104, 301, 767, 1220
TatI WGTACW 5 cut(s) 145, 343, 438, 838, 858
TauI GCSGC 3 cut(s) 143, 380, 474
TfiI GAWTC 3 cut(s) 46, 982, 1282
Tru1I TTAA 3 cut(s) 56, 387, 921
Tru9I TTAA 3 cut(s) 56, 387, 921
TscAI CASTG 1 cut(s) 519
TseFI GTSAC 1 cut(s) 670
TseI GCWGC 5 cut(s) 170, 314, 338, 518, 898
Tsp45I GTSAC 1 cut(s) 670
TspDTI ATGAA 3 cut(s) 104, 137, 517
TspRI CASTG 1 cut(s) 519
Tth111I GACNNNGTC 1 cut(s) 1199
VpaK11BI GGWCC 2 cut(s) 35, 1181
XapI RAATTY 3 cut(s) 389, 808, 1386
XbaI TCTAGA 1 cut(s) 1288
XceI RCATGY 1 cut(s) 177
XcmI CCANNNNNNNNNTGG 1 cut(s) 577
XmaJI CCTAGG 1 cut(s) 1318
XmiI GTMKAC 1 cut(s) 162
XmnI GAANNNNTTC 1 cut(s) 462
XspI CTAG 3 cut(s) 1085, 1289, 1319
ZrmI AGTACT 1 cut(s) 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.