Rh4DG239700

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000950
Physical Location & Seq
Forward (+)
44777877 .. 44801532
23656 bp
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UTR
Exon/CDS
Intron
Rh4DG239700.1

Sequence Viewer

Length: 1488 bp
ATGTGCAAGCTCTTCAGTTCAGTGAGAAAGTGGATCGGAAACTCCCTCTACTTTTCCAAGGCTACTTTATGCGTCCGGGGTTTGGTTTTCAGTCAGGCCTTTGTAGGCCGTGCGGTGTCGTCCATCTTGATCACGTACCTCGTACTTCATATTTCGAAGAACTATCAAATGGCTGCGATACTTATAAATTTGGTATTTGGGGCAGCATCCGTCATGGTCATCGTCCTTGCTTATCTATCACACAAAATAGGCCATTTTAGAATGGTTGTCTTCTCAAGTTTAGCCTATACTCTTGCACTGGCTCTATTATGGATTTCAGTCCAGTTTATACGTCAGGGCACAGATGCAGTAGATGATTTTTTCTATTGTGCCGCGGTACTATTGGCAATAGGTGTCGCTGGAATGGATGCTGTTATACCTAAATTTCTTGACGATCAATACACAAGTGAACAGAAACAAAGAGATCATGATTCAGAAGAAGAAAAAGAACGATTGCAAAGCCGCAAAGATGCTTTGTGGAGCCATCCCGCTTTTTTCGGTGCAGTGGCTGCTCTTTTCATTCCTGACGAGGCACCTTGGGAAACTGTCTTCCTGATCTCCATGATTGCAATGGGGGTTTGTTTATTATTGTTCTGGTTTGGCAATTCCTTGTATTCAGACCCATATTCCGAAGAAAATTCAGACCCATCTTCAGAAGAAACAGCACCAAGAAGGAAGATTGGGGTCACACTGAAAGAAAATGGACACCGCTATGCAGAAATGTTAGCTATAAGCTTGGCCTTCGTTGCATACAGTCTGGTGGAGGCAACGGGATGCACGTTCTTTTTTGAACAAACAGATTACTTGGACAATTGTGTCGGAAATTTCACAGTTCCTGTCGTATATCTCACTGTACTTCAGTCCTTTTCAAAGTTCATAATCCCGTATCTTTGGGGGTTACTAATTCCAAAGCAGGGAAGAAGTGTTGCATTGGTTAAAATTGGGTGCGGTTTGGTTCTTTCTGTTTTATGCTGTGTCATTGCTTGGCGAATTGAGATTCGTAGGAAGAGTAAAATCATGGTGGAAGTTGACCCTGAAGCAGATTACAACCAAATTATTTCCATGAGCATCCTTTGGTTACTTCCACAATTCTTTATGCTAGGGCTGATGAGAGGGTTAGCTGAAGACGGATTTAACGAGTTTTTTGCGGAACAAGTCGTTGACGATGATAAAGAAGTGGTGCTTAGGTACTATGGACCTCACGTATTTTCCTTTGTCCTGGGTATTGGAACATTACTGACTGCTGTTAGTATTTTGGTGTTTAGACGAACTTGGTTGGATCATAATATATATGAGAATCGTCTAGATAAGTATTACAGGGGATTAGTATTCCTAGGTGTTGCTAACCTTGGCTGCTACTTGCGTGTCGCCCTTTACATTTACACTAAGCATGAATCAGAAATTTCTGAAGAAACAAATGAAGAAACTGCAAAAGAGGGAGAGATTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

495

Amino Acids

55.98

Weight (kDa)

5.42

Isoelectric Point (pI)

35.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 33 - 342 1e-13 Major Facilitator Superfamily
PTR2 PF00854 86 - 226 1.7e-07 POT family
PTR2 PF00854 244 - 403 2.4e-07 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 185
AasI GACNNNNNNGTC 1 cut(s) 854
AccB1I GGYRCC 1 cut(s) 571
AccII CGCG 1 cut(s) 374
AciI CCGC 8 cut(s) 113, 372, 374, 502, 528, 748, 987, 1187
AclWI GGATC 2 cut(s) 41, 1326
AcsI RAATTY 5 cut(s) 187, 422, 676, 862, 1440
AcuI CTGAAG 5 cut(s) 675, 881, 1095, 1182, 1467
AfaI GTAC 5 cut(s) 137, 144, 378, 894, 1229
AfiI CCNNNNNNNGG 2 cut(s) 82, 953
AgsI TTSAA 2 cut(s) 830, 909
AjnI CCWGG 1 cut(s) 1257
AluBI AGCT 4 cut(s) 10, 767, 774, 1160
AluI AGCT 4 cut(s) 10, 767, 774, 1160
AlwI GGATC 2 cut(s) 41, 1326
AlwNI CAGNNNCTG 2 cut(s) 548, 875
AoxI GGCC 4 cut(s) 96, 106, 250, 777
ApeKI GCWGC 4 cut(s) 173, 203, 548, 1392
ApoI RAATTY 5 cut(s) 187, 422, 676, 862, 1440
AspA2I CCTAGG 1 cut(s) 1372
AspS9I GGNCC 1 cut(s) 1235
AsuC2I CCSGG 1 cut(s) 77
AsuII TTCGAA 1 cut(s) 155
AvaII GGWCC 1 cut(s) 1235
AvrII CCTAGG 1 cut(s) 1372
BaeGI GKGCMC 1 cut(s) 341
BanI GGYRCC 1 cut(s) 571
BbsI GAAGAC 3 cut(s) 262, 580, 1170
BbvI GCAGC 4 cut(s) 160, 215, 535, 1379
BccI CCATC 3 cut(s) 131, 531, 694
BceAI ACGGC 1 cut(s) 93
BcgI CGANNNNNNTGC 2 cut(s) 1166, 1200
BciT130I CCWGG 1 cut(s) 1259
BclI TGATCA 1 cut(s) 129
BcnI CCSGG 1 cut(s) 77
BfaI CTAG 3 cut(s) 1139, 1343, 1373
BisI GCNGC 6 cut(s) 174, 204, 372, 502, 549, 1393
BlnI CCTAGG 1 cut(s) 1372
BlsI GCNGC 6 cut(s) 175, 205, 373, 503, 550, 1394
Bme1390I CCNGG 2 cut(s) 77, 1259
Bme18I GGWCC 1 cut(s) 1235
BmgT120I GGNCC 1 cut(s) 1235
BmiI GGNNCC 2 cut(s) 521, 573
BmrFI CCNGG 2 cut(s) 77, 1259
BmsI GCATC 6 cut(s) 215, 334, 397, 499, 803, 1116
BpiI GAAGAC 3 cut(s) 262, 580, 1170
Bpu10I CCTNAGC 1 cut(s) 1223
Bpu14I TTCGAA 1 cut(s) 155
BpuEI CTTGAG 1 cut(s) 259
BpuMI CCSGG 1 cut(s) 77
BsaAI YACGTR 2 cut(s) 135, 1243
BsaJI CCNNGG 7 cut(s) 57, 76, 372, 575, 1258, 1372, 1387
Bsc4I CCNNNNNNNGG 2 cut(s) 82, 953
Bse1I ACTGG 2 cut(s) 303, 322
Bse3DI GCAATG 2 cut(s) 615, 1017
BseBI CCWGG 1 cut(s) 1259
BseDI CCNNGG 7 cut(s) 57, 76, 372, 575, 1258, 1372, 1387
BseGI GGATG 5 cut(s) 206, 412, 523, 818, 1107
BseLI CCNNNNNNNGG 2 cut(s) 82, 953
BseMI GCAATG 2 cut(s) 615, 1017
BseNI ACTGG 2 cut(s) 303, 322
BseSI GKGCMC 1 cut(s) 341
BseXI GCAGC 4 cut(s) 160, 215, 535, 1379
BsgI GTGCAG 1 cut(s) 561
Bsh1236I CGCG 1 cut(s) 374
BshFI GGCC 4 cut(s) 98, 108, 252, 779
BshNI GGYRCC 1 cut(s) 571
BsiSI CCGG 1 cut(s) 76
BslI CCNNNNNNNGG 2 cut(s) 82, 953
BsnI GGCC 4 cut(s) 98, 108, 252, 779
Bsp119I TTCGAA 1 cut(s) 155
Bsp1286I GDGCHC 1 cut(s) 341
Bsp143I GATC 6 cut(s) 33, 129, 433, 463, 594, 1318
BspACI CCGC 8 cut(s) 113, 372, 374, 502, 528, 748, 987, 1187
BspANI GGCC 4 cut(s) 98, 108, 252, 779
BspFNI CGCG 1 cut(s) 374
BspHI TCATGA 1 cut(s) 466
BspLI GGNNCC 2 cut(s) 521, 573
BspPI GGATC 2 cut(s) 41, 1326
BspQI GCTCTTC 1 cut(s) 17
BspT104I TTCGAA 1 cut(s) 155
BspT107I GGYRCC 1 cut(s) 571
BsrDI GCAATG 2 cut(s) 615, 1017
BsrI ACTGG 2 cut(s) 303, 322
BssECI CCNNGG 7 cut(s) 57, 76, 372, 575, 1258, 1372, 1387
BssMI GATC 6 cut(s) 33, 129, 433, 463, 594, 1318
BssT1I CCWWGG 4 cut(s) 57, 575, 1372, 1387
Bst2UI CCWGG 1 cut(s) 1259
Bst4CI ACNGT 4 cut(s) 586, 794, 871, 892
Bst6I CTCTTC 2 cut(s) 17, 1040
BstAPI GCANNNNNTGC 1 cut(s) 548
BstBAI YACGTR 2 cut(s) 135, 1243
BstBI TTCGAA 1 cut(s) 155
BstC8I GCNNGC 1 cut(s) 8
BstDEI CTNAG 2 cut(s) 1223, 1425
BstDSI CCRYGG 1 cut(s) 372
BstF5I GGATG 5 cut(s) 206, 412, 523, 818, 1107
BstFNI CGCG 1 cut(s) 374
BstKTI GATC 6 cut(s) 36, 132, 436, 466, 597, 1321
BstMBI GATC 6 cut(s) 33, 129, 433, 463, 594, 1318
BstMWI GCNNNNNNNGC 2 cut(s) 548, 785
BstNI CCWGG 1 cut(s) 1259
BstSCI CCNGG 2 cut(s) 75, 1257
BstSLI GKGCMC 1 cut(s) 341
BstUI CGCG 1 cut(s) 374
BstV1I GCAGC 4 cut(s) 160, 215, 535, 1379
BstV2I GAAGAC 3 cut(s) 262, 580, 1170
BsuRI GGCC 4 cut(s) 98, 108, 252, 779
BtgI CCRYGG 1 cut(s) 372
BtsCI GGATG 5 cut(s) 206, 412, 523, 818, 1107
BtsI GCAGTG 1 cut(s) 549
BtsIMutI CAGTG 5 cut(s) 27, 296, 549, 728, 888
Cac8I GCNNGC 1 cut(s) 8
CaiI CAGNNNCTG 2 cut(s) 548, 875
CciI TCATGA 1 cut(s) 466
Cfr13I GGNCC 1 cut(s) 1235
Cfr42I CCGCGG 1 cut(s) 375
CseI GACGC 1 cut(s) 61
Csp6I GTAC 5 cut(s) 136, 143, 377, 893, 1228
CviAII CATG 6 cut(s) 214, 467, 601, 1057, 1102, 1430
CviQI GTAC 5 cut(s) 136, 143, 377, 893, 1228
DdeI CTNAG 2 cut(s) 1223, 1425
DpnI GATC 6 cut(s) 35, 131, 435, 465, 596, 1320
DpnII GATC 6 cut(s) 33, 129, 433, 463, 594, 1318
DrdI GACNNNNNNGTC 1 cut(s) 854
DseDI GACNNNNNNGTC 1 cut(s) 854
Eam1104I CTCTTC 2 cut(s) 17, 1040
EarI CTCTTC 2 cut(s) 17, 1040
Eco130I CCWWGG 4 cut(s) 57, 575, 1372, 1387
Eco147I AGGCCT 1 cut(s) 98
Eco47I GGWCC 1 cut(s) 1235
Eco57I CTGAAG 5 cut(s) 675, 881, 1095, 1182, 1467
EcoRII CCWGG 1 cut(s) 1257
EcoT14I CCWWGG 4 cut(s) 57, 575, 1372, 1387
ErhI CCWWGG 4 cut(s) 57, 575, 1372, 1387
FaeI CATG 6 cut(s) 217, 470, 604, 1060, 1105, 1433
FalI AAGNNNNNCTT 2 cut(s) 1206, 1238
FatI CATG 6 cut(s) 213, 466, 600, 1056, 1101, 1429
FauI CCCGC 1 cut(s) 535
FbaI TGATCA 1 cut(s) 129
Fnu4HI GCNGC 6 cut(s) 174, 204, 372, 502, 549, 1393
FokI GGATG 5 cut(s) 193, 419, 510, 825, 1094
Fsp4HI GCNGC 6 cut(s) 174, 204, 372, 502, 549, 1393
FspBI CTAG 3 cut(s) 1139, 1343, 1373
GluI GCNGC 6 cut(s) 174, 204, 372, 502, 549, 1393
HaeIII GGCC 4 cut(s) 98, 108, 252, 779
HapII CCGG 1 cut(s) 76
HgaI GACGC 1 cut(s) 61
Hin1II CATG 6 cut(s) 217, 470, 604, 1060, 1105, 1433
HincII GTYRAC 2 cut(s) 1069, 1201
HindII GTYRAC 2 cut(s) 1069, 1201
HindIII AAGCTT 1 cut(s) 772
HinfI GANTC 4 cut(s) 470, 1036, 1336, 1433
HpaII CCGG 1 cut(s) 76
Hpy166II GTNNAC 3 cut(s) 449, 1069, 1201
Hpy188I TCNGA 9 cut(s) 38, 475, 658, 670, 682, 694, 860, 1438, 1447
Hpy188III TCNNGA 6 cut(s) 127, 428, 467, 563, 592, 1343
Hpy8I GTNNAC 3 cut(s) 449, 1069, 1201
HpyAV CCTTC 2 cut(s) 705, 790
HpyCH4III ACNGT 4 cut(s) 586, 794, 871, 892
HpyCH4IV ACGT 4 cut(s) 134, 331, 818, 1242
HpyF10VI GCNNNNNNNGC 2 cut(s) 548, 785
HpyF3I CTNAG 2 cut(s) 1223, 1425
HpySE526I ACGT 4 cut(s) 134, 331, 818, 1242
Hsp92II CATG 6 cut(s) 217, 470, 604, 1060, 1105, 1433
Ksp22I TGATCA 1 cut(s) 129
KspI CCGCGG 1 cut(s) 375
Kzo9I GATC 6 cut(s) 33, 129, 433, 463, 594, 1318
LguI GCTCTTC 1 cut(s) 17
LmnI GCTCC 1 cut(s) 519
Lsp1109I GCAGC 4 cut(s) 160, 215, 535, 1379
LweI GCATC 6 cut(s) 215, 334, 397, 499, 803, 1116
MaeI CTAG 3 cut(s) 1139, 1343, 1373
MaeII ACGT 4 cut(s) 134, 331, 818, 1242
MaeIII GTNAC 3 cut(s) 724, 936, 1116
MalI GATC 6 cut(s) 35, 131, 435, 465, 596, 1320
MboI GATC 6 cut(s) 33, 129, 433, 463, 594, 1318
MfeI CAATTG 1 cut(s) 850
MhlI GDGCHC 1 cut(s) 341
MmeI TCCRAC 2 cut(s) 838, 1296
MnlI CCTC 7 cut(s) 56, 149, 562, 796, 1145, 1248, 1468
MseI TTAA 2 cut(s) 975, 1173
MslI CAYNNNNRTG 1 cut(s) 750
MspA1I CMGCKG 1 cut(s) 374
MspI CCGG 1 cut(s) 76
MspR9I CCNGG 2 cut(s) 77, 1259
MunI CAATTG 1 cut(s) 850
MvaI CCWGG 1 cut(s) 1259
MvnI CGCG 1 cut(s) 374
MwoI GCNNNNNNNGC 2 cut(s) 548, 785
NciI CCSGG 1 cut(s) 77
NdeII GATC 6 cut(s) 33, 129, 433, 463, 594, 1318
NlaIII CATG 6 cut(s) 217, 470, 604, 1060, 1105, 1433
NlaIV GGNNCC 2 cut(s) 521, 573
NmuCI GTSAC 1 cut(s) 724
NspV TTCGAA 1 cut(s) 155
PagI TCATGA 1 cut(s) 466
PceI AGGCCT 1 cut(s) 98
PciSI GCTCTTC 1 cut(s) 17
PcsI WCGNNNNNNNCGW 2 cut(s) 815, 1173
PfeI GAWTC 4 cut(s) 470, 1036, 1336, 1433
PkrI GCNGC 6 cut(s) 175, 205, 373, 503, 550, 1394
Ppu21I YACGTR 2 cut(s) 135, 1243
PsiI TTATAA 1 cut(s) 185
Psp6I CCWGG 1 cut(s) 1257
PspGI CCWGG 1 cut(s) 1257
PspN4I GGNNCC 2 cut(s) 521, 573
PspPI GGNCC 1 cut(s) 1235
PstNI CAGNNNCTG 2 cut(s) 548, 875
RsaI GTAC 5 cut(s) 137, 144, 378, 894, 1229
RsaNI GTAC 5 cut(s) 136, 143, 377, 893, 1228
RseI CAYNNNNRTG 1 cut(s) 750
SacII CCGCGG 1 cut(s) 375
SapI GCTCTTC 1 cut(s) 17
SaqAI TTAA 2 cut(s) 975, 1173
SatI GCNGC 6 cut(s) 174, 204, 372, 502, 549, 1393
Sau3AI GATC 6 cut(s) 33, 129, 433, 463, 594, 1318
Sau96I GGNCC 1 cut(s) 1235
ScrFI CCNGG 2 cut(s) 77, 1259
SduI GDGCHC 1 cut(s) 341
SfaNI GCATC 6 cut(s) 215, 334, 397, 499, 803, 1116
Sfr303I CCGCGG 1 cut(s) 375
SfuI TTCGAA 1 cut(s) 155
SgrBI CCGCGG 1 cut(s) 375
SinI GGWCC 1 cut(s) 1235
SmiMI CAYNNNNRTG 1 cut(s) 750
SmlI CTYRAG 1 cut(s) 274
SmoI CTYRAG 1 cut(s) 274
SseBI AGGCCT 1 cut(s) 98
SsiI CCGC 8 cut(s) 113, 372, 374, 502, 528, 748, 987, 1187
SspMI CTAG 3 cut(s) 1139, 1343, 1373
StuI AGGCCT 1 cut(s) 98
StyD4I CCNGG 2 cut(s) 75, 1257
StyI CCWWGG 4 cut(s) 57, 575, 1372, 1387
TaaI ACNGT 4 cut(s) 586, 794, 871, 892
TaiI ACGT 4 cut(s) 137, 334, 821, 1245
TaqI TCGA 1 cut(s) 155
TatI WGTACW 1 cut(s) 892
TauI GCSGC 2 cut(s) 374, 504
TfiI GAWTC 4 cut(s) 470, 1036, 1336, 1433
Tru1I TTAA 2 cut(s) 975, 1173
Tru9I TTAA 2 cut(s) 975, 1173
TscAI CASTG 5 cut(s) 27, 303, 549, 735, 895
TseFI GTSAC 1 cut(s) 724
TseI GCWGC 4 cut(s) 173, 203, 548, 1392
Tsp45I GTSAC 1 cut(s) 724
TspDTI ATGAA 5 cut(s) 137, 547, 904, 1446, 1473
TspGWI ACGGA 2 cut(s) 199, 1182
TspRI CASTG 5 cut(s) 27, 303, 549, 735, 895
VpaK11BI GGWCC 1 cut(s) 1235
XapI RAATTY 5 cut(s) 187, 422, 676, 862, 1440
XbaI TCTAGA 1 cut(s) 1342
XcmI CCANNNNNNNNNTGG 1 cut(s) 607
XmaJI CCTAGG 1 cut(s) 1372
XspI CTAG 3 cut(s) 1139, 1343, 1373
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.