Rh4DG123600

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0000950
Physical Location & Seq
Forward (+)
19883367 .. 19888015
4649 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG123600.1

Sequence Viewer

Length: 1566 bp
ATGGCTGCGGCACTTATAAATTTGATTGTTGGGGCAGCATCCGTCATGGTCATCGTCCTTGCTTATCTATCACACAGAATAGGTCATTTTAGAATGGTTGTCTCCGCAAGTTTTGCCTATACTCTTGCACTGGCTCTATTATGGATTTCAGCCAAGTTTATACGTCAGGGAGCAGATGCAGTAGATAATTTTTTCTATTGGGCCGCGGTACTATTGGCAATAGCTGGCGCTGGAATGGATGCTGTTATACCTAAATTTCTTGACGATCAATACACAAGTGAACAGAGAGAAAGAGATCATGAATACACAATAGAAGAAGAAGAACGATTCCAAAGCCGCAAAGATGCTTTGTGGAGCCATCCCTGGTTTTTCGGTGCAGTGGCAGCTCTTTTCATTCCTGGAGAGGCACATTGGAAAACTGTCTTCCTGATTTCAATGATTGCAATGGGGGTTTGTTTTTTATTGTTCTGGTTTGGCAAATCCTTTTATTCAGTGTCATCTTCAGTAGAAACAGCACCAAGAGGGAAGTTTGGGGTCACACTGAAAGAAAATGGACACCGTTATGCAGAAATGTTGGCTATGAGCTTTGCCTTCATTGCATACAGTCTGGTGGAGGCAACAGGATCCACGTTCTTTTTTGAACAAACAACTTTCTTGGATGATCATGTCGGAAAATTCACAGTTCCTGTCGTATATCTCACTGTACTTCAGTCTTTTTCAAAGTTCATAATCCCACATCTTTGGGGGCTACTAATTCCAAAGCAGCGAAGAAGTGTTGCATTGGTTAAAATTGGGTGCGGTTTGGTTCTTTCTGTTTTATGCTGCGTCATTGCTTGGCGAATTGAGATTCGTAGGTCGAGTAAAATCAAGGTGGAAGTTGACCTTGAAGATTACAAACCAATTATTTCCATGAGCATCCTTTGGTTACTTCCGCAATTCTCTCTGCTTGGGCTCATGAGAGGGTTAGCTGAAGACGGATTGGTCGAGTTTTTTGCAGAACAAGTGGTTGACGATGATAAACAAGCGGTGCTTAGGTACTATGGACCTCACGTATTTTCCTTTGTCCTGGGTATTGGAACACTACTGACCGCTGTTAGTATTTTGGGTTTTAGACGAACTTGGTTGGATGATGATATATATAAGAATCGTCTAGATAAGTATTACAGGGGATTAGTATTCCTAGGTGTTGCTAACCTTGGCTACTACTTGTGTATCGCCCTATACTTTTACAAAAAGCATGGATCACAAATTTCTGCTGAAGAAACAAATGATGAAGAAACTGCAACAGACCTTGAACCTCCTCCTCAAGTAATAATAACCAGAGAGCCTGATGATATTGTTATTCCTGGATTGAATTTCCGACCAGACCCACGTGGATTTTGGTGTCCCCTTCGCCTTGACGATCAAATTCCACGGCGTCCGATCAGCGTAAAAGACCAGAATCTCATCACCGGGGCTGGCCTGACCCTCGTCCAAACAAACCCGACCTTCAGTTCCTTGAACTCGTCAGAGATGGTGAACTTGGAGGCGCCGTCGAGGTCACCGATCTCCTCGTCAGAGATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

521

Amino Acids

58.66

Weight (kDa)

5.76

Isoelectric Point (pI)

35.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 181 - 338 1.1e-10 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 17
AasI GACNNNNNNGTC 1 cut(s) 980
AccB1I GGYRCC 1 cut(s) 1528
AccII CGCG 1 cut(s) 206
AciI CCGC 9 cut(s) 8, 105, 204, 206, 337, 798, 932, 1025, 1089
AclWI GGATC 3 cut(s) 618, 631, 1249
AcsI RAATTY 6 cut(s) 19, 254, 674, 1248, 1354, 1407
AcuI CTGAAG 5 cut(s) 486, 692, 990, 1278, 1474
AcvI CACGTG 1 cut(s) 1373
AcyI GRCGYC 2 cut(s) 1417, 1529
AfaI GTAC 3 cut(s) 210, 705, 1037
AgsI TTSAA 7 cut(s) 435, 641, 720, 887, 1295, 1354, 1501
AjnI CCWGG 4 cut(s) 362, 397, 1065, 1345
AluBI AGCT 4 cut(s) 224, 386, 585, 968
AluI AGCT 4 cut(s) 224, 386, 585, 968
Alw26I GTCTC 1 cut(s) 106
AlwI GGATC 3 cut(s) 618, 631, 1249
AlwNI CAGNNNCTG 1 cut(s) 686
AoxI GGCC 2 cut(s) 201, 1459
ApeKI GCWGC 5 cut(s) 5, 35, 383, 763, 822
ApoI RAATTY 6 cut(s) 19, 254, 674, 1248, 1354, 1407
AspA2I CCTAGG 1 cut(s) 1180
AspLEI GCGC 2 cut(s) 230, 1531
AspS9I GGNCC 2 cut(s) 201, 1043
AsuC2I CCSGG 1 cut(s) 1453
AsuHPI GGTGA 3 cut(s) 1441, 1528, 1533
AvaII GGWCC 1 cut(s) 1043
AvrII CCTAGG 1 cut(s) 1180
BaeI ACNNNNGTAYC 2 cut(s) 1195, 1228
BamHI GGATCC 1 cut(s) 623
BanI GGYRCC 1 cut(s) 1528
BanII GRGCYC 1 cut(s) 954
BbrPI CACGTG 1 cut(s) 1373
BbsI GAAGAC 2 cut(s) 415, 978
BbvI GCAGC 4 cut(s) 47, 395, 775, 809
BccI CCATC 2 cut(s) 366, 1507
BceAI ACGGC 2 cut(s) 1430, 1516
BcgI CGANNNNNNTGC 2 cut(s) 974, 1008
BciT130I CCWGG 4 cut(s) 364, 399, 1067, 1347
BclI TGATCA 1 cut(s) 661
BcnI CCSGG 1 cut(s) 1453
BcoDI GTCTC 1 cut(s) 106
BfaI CTAG 2 cut(s) 1151, 1181
BfoI RGCGCY 2 cut(s) 231, 1532
BisI GCNGC 8 cut(s) 6, 9, 36, 204, 337, 384, 764, 823
BlnI CCTAGG 1 cut(s) 1180
BlsI GCNGC 8 cut(s) 7, 10, 37, 205, 338, 385, 765, 824
Bme1390I CCNGG 5 cut(s) 364, 399, 1067, 1347, 1453
Bme18I GGWCC 1 cut(s) 1043
BmgT120I GGNCC 2 cut(s) 201, 1043
BmiI GGNNCC 3 cut(s) 356, 625, 1530
BmrFI CCNGG 5 cut(s) 364, 399, 1067, 1347, 1453
BmsI GCATC 5 cut(s) 47, 166, 229, 334, 924
BoxI GACNNNNGTC 1 cut(s) 1469
BpiI GAAGAC 2 cut(s) 415, 978
BpmI CTGGAG 1 cut(s) 420
Bpu10I CCTNAGC 1 cut(s) 1031
BpuEI CTTGAG 1 cut(s) 1290
BpuMI CCSGG 1 cut(s) 1453
BsaAI YACGTR 2 cut(s) 1051, 1373
BsaHI GRCGYC 2 cut(s) 1417, 1529
BsaJI CCNNGG 7 cut(s) 204, 362, 1066, 1180, 1195, 1412, 1452
BsaXI ACNNNNNCTCC 2 cut(s) 1517, 1547
Bse1I ACTGG 1 cut(s) 135
Bse3DI GCAATG 3 cut(s) 450, 594, 828
BseBI CCWGG 4 cut(s) 364, 399, 1067, 1347
BseDI CCNNGG 7 cut(s) 204, 362, 1066, 1180, 1195, 1412, 1452
BseGI GGATG 6 cut(s) 38, 244, 358, 664, 915, 1132
BseMI GCAATG 3 cut(s) 450, 594, 828
BseNI ACTGG 1 cut(s) 135
BseRI GAGGAG 3 cut(s) 1290, 1293, 1540
BseXI GCAGC 4 cut(s) 47, 395, 775, 809
BsgI GTGCAG 1 cut(s) 396
Bsh1236I CGCG 1 cut(s) 206
BshFI GGCC 2 cut(s) 203, 1461
BshNI GGYRCC 1 cut(s) 1528
BsiSI CCGG 1 cut(s) 1452
BslFI GGGAC 1 cut(s) 1371
BsmAI GTCTC 1 cut(s) 106
BsmFI GGGAC 1 cut(s) 1371
BsnI GGCC 2 cut(s) 203, 1461
Bsp1286I GDGCHC 1 cut(s) 954
Bsp143I GATC 8 cut(s) 265, 295, 623, 661, 1241, 1402, 1422, 1545
BspACI CCGC 9 cut(s) 8, 105, 204, 206, 337, 798, 932, 1025, 1089
BspANI GGCC 2 cut(s) 203, 1461
BspFNI CGCG 1 cut(s) 206
BspHI TCATGA 2 cut(s) 298, 954
BspLI GGNNCC 3 cut(s) 356, 625, 1530
BspPI GGATC 3 cut(s) 618, 631, 1249
BspT107I GGYRCC 1 cut(s) 1528
BsrDI GCAATG 3 cut(s) 450, 594, 828
BsrI ACTGG 1 cut(s) 135
BssECI CCNNGG 7 cut(s) 204, 362, 1066, 1180, 1195, 1412, 1452
BssMI GATC 8 cut(s) 265, 295, 623, 661, 1241, 1402, 1422, 1545
BssNI GRCGYC 2 cut(s) 1417, 1529
BssT1I CCWWGG 2 cut(s) 1180, 1195
Bst2UI CCWGG 4 cut(s) 364, 399, 1067, 1347
Bst4CI ACNGT 5 cut(s) 421, 560, 605, 682, 703
BstACI GRCGYC 2 cut(s) 1417, 1529
BstAPI GCANNNNNTGC 1 cut(s) 113
BstBAI YACGTR 2 cut(s) 1051, 1373
BstC8I GCNNGC 2 cut(s) 226, 1459
BstDEI CTNAG 1 cut(s) 1031
BstDSI CCRYGG 2 cut(s) 204, 1412
BstEII GGTNACC 1 cut(s) 1539
BstF5I GGATG 6 cut(s) 38, 244, 358, 664, 915, 1132
BstFNI CGCG 1 cut(s) 206
BstH2I RGCGCY 2 cut(s) 231, 1532
BstHHI GCGC 2 cut(s) 230, 1531
BstKTI GATC 8 cut(s) 268, 298, 626, 664, 1244, 1405, 1425, 1548
BstMAI GTCTC 1 cut(s) 106
BstMBI GATC 8 cut(s) 265, 295, 623, 661, 1241, 1402, 1422, 1545
BstMWI GCNNNNNNNGC 3 cut(s) 113, 383, 596
BstNI CCWGG 4 cut(s) 364, 399, 1067, 1347
BstPAI GACNNNNGTC 1 cut(s) 1469
BstPI GGTNACC 1 cut(s) 1539
BstSCI CCNGG 5 cut(s) 362, 397, 1065, 1345, 1451
BstUI CGCG 1 cut(s) 206
BstV1I GCAGC 4 cut(s) 47, 395, 775, 809
BstV2I GAAGAC 2 cut(s) 415, 978
BstX2I RGATCY 1 cut(s) 623
BstXI CCANNNNNNTGG 1 cut(s) 741
BstYI RGATCY 1 cut(s) 623
BsuRI GGCC 2 cut(s) 203, 1461
BtgI CCRYGG 2 cut(s) 204, 1412
BtsCI GGATG 6 cut(s) 38, 244, 358, 664, 915, 1132
BtsI GCAGTG 1 cut(s) 384
BtsIMutI CAGTG 5 cut(s) 128, 384, 498, 539, 699
Cac8I GCNNGC 2 cut(s) 226, 1459
CaiI CAGNNNCTG 1 cut(s) 686
CciI TCATGA 2 cut(s) 298, 954
CfoI GCGC 2 cut(s) 230, 1531
Cfr13I GGNCC 2 cut(s) 201, 1043
Cfr42I CCGCGG 1 cut(s) 207
CseI GACGC 2 cut(s) 814, 1406
Csp6I GTAC 3 cut(s) 209, 704, 1036
CviAII CATG 6 cut(s) 46, 299, 665, 910, 955, 1238
CviQI GTAC 3 cut(s) 209, 704, 1036
DdeI CTNAG 1 cut(s) 1031
DinI GGCGCC 1 cut(s) 1530
DpnI GATC 8 cut(s) 267, 297, 625, 663, 1243, 1404, 1424, 1547
DpnII GATC 8 cut(s) 265, 295, 623, 661, 1241, 1402, 1422, 1545
DrdI GACNNNNNNGTC 1 cut(s) 980
DseDI GACNNNNNNGTC 1 cut(s) 980
Eco130I CCWWGG 2 cut(s) 1180, 1195
Eco24I GRGCYC 1 cut(s) 954
Eco47I GGWCC 1 cut(s) 1043
Eco57I CTGAAG 5 cut(s) 486, 692, 990, 1278, 1474
Eco72I CACGTG 1 cut(s) 1373
Eco91I GGTNACC 1 cut(s) 1539
EcoO65I GGTNACC 1 cut(s) 1539
EcoRII CCWGG 4 cut(s) 362, 397, 1065, 1345
EcoT14I CCWWGG 2 cut(s) 1180, 1195
EcoT38I GRGCYC 1 cut(s) 954
EgeI GGCGCC 1 cut(s) 1530
EheI GGCGCC 1 cut(s) 1530
ErhI CCWWGG 2 cut(s) 1180, 1195
FaeI CATG 6 cut(s) 49, 302, 668, 913, 958, 1241
FalI AAGNNNNNCTT 4 cut(s) 867, 899, 1014, 1046
FaqI GGGAC 1 cut(s) 1371
FatI CATG 6 cut(s) 45, 298, 664, 909, 954, 1237
FbaI TGATCA 1 cut(s) 661
Fnu4HI GCNGC 8 cut(s) 6, 9, 36, 204, 337, 384, 764, 823
FokI GGATG 6 cut(s) 25, 251, 345, 671, 902, 1139
FriOI GRGCYC 1 cut(s) 954
Fsp4HI GCNGC 8 cut(s) 6, 9, 36, 204, 337, 384, 764, 823
FspBI CTAG 2 cut(s) 1151, 1181
GlaI GCGC 2 cut(s) 229, 1530
GluI GCNGC 8 cut(s) 6, 9, 36, 204, 337, 384, 764, 823
GsuI CTGGAG 1 cut(s) 420
HaeII RGCGCY 2 cut(s) 231, 1532
HaeIII GGCC 2 cut(s) 203, 1461
HapII CCGG 1 cut(s) 1452
HgaI GACGC 2 cut(s) 814, 1406
HhaI GCGC 2 cut(s) 230, 1531
Hin1I GRCGYC 2 cut(s) 1417, 1529
Hin1II CATG 6 cut(s) 49, 302, 668, 913, 958, 1241
Hin6I GCGC 2 cut(s) 228, 1529
HinP1I GCGC 2 cut(s) 228, 1529
HincII GTYRAC 2 cut(s) 880, 1009
HindII GTYRAC 2 cut(s) 880, 1009
HinfI GANTC 4 cut(s) 327, 847, 1144, 1441
HpaII CCGG 1 cut(s) 1452
HphI GGTGA 3 cut(s) 1441, 1528, 1533
Hpy166II GTNNAC 4 cut(s) 281, 880, 1009, 1519
Hpy188I TCNGA 5 cut(s) 671, 1361, 1422, 1510, 1558
Hpy188III TCNNGA 5 cut(s) 260, 299, 427, 955, 1151
Hpy8I GTNNAC 4 cut(s) 281, 880, 1009, 1519
Hpy99I CGWCG 1 cut(s) 1537
HpyAV CCTTC 3 cut(s) 601, 1400, 1498
HpyCH4III ACNGT 5 cut(s) 421, 560, 605, 682, 703
HpyCH4IV ACGT 4 cut(s) 163, 629, 1050, 1372
HpyCH4V TGCA 9 cut(s) 128, 179, 377, 443, 566, 599, 779, 995, 1283
HpyF10VI GCNNNNNNNGC 3 cut(s) 113, 383, 596
HpyF3I CTNAG 1 cut(s) 1031
HpySE526I ACGT 4 cut(s) 163, 629, 1050, 1372
Hsp92I GRCGYC 2 cut(s) 1417, 1529
Hsp92II CATG 6 cut(s) 49, 302, 668, 913, 958, 1241
HspAI GCGC 2 cut(s) 228, 1529
KasI GGCGCC 1 cut(s) 1528
Ksp22I TGATCA 1 cut(s) 661
KspI CCGCGG 1 cut(s) 207
Kzo9I GATC 8 cut(s) 265, 295, 623, 661, 1241, 1402, 1422, 1545
LmnI GCTCC 2 cut(s) 170, 354
Lsp1109I GCAGC 4 cut(s) 47, 395, 775, 809
LweI GCATC 5 cut(s) 47, 166, 229, 334, 924
MaeI CTAG 2 cut(s) 1151, 1181
MaeII ACGT 4 cut(s) 163, 629, 1050, 1372
MaeIII GTNAC 3 cut(s) 535, 924, 1539
MalI GATC 8 cut(s) 267, 297, 625, 663, 1243, 1404, 1424, 1547
MboI GATC 8 cut(s) 265, 295, 623, 661, 1241, 1402, 1422, 1545
MflI RGATCY 1 cut(s) 623
MhlI GDGCHC 1 cut(s) 954
Mly113I GGCGCC 1 cut(s) 1529
MmeI TCCRAC 3 cut(s) 649, 1104, 1384
MseI TTAA 1 cut(s) 786
MslI CAYNNNNRTG 1 cut(s) 561
MspA1I CMGCKG 2 cut(s) 206, 1091
MspI CCGG 1 cut(s) 1452
MspR9I CCNGG 5 cut(s) 364, 399, 1067, 1347, 1453
MvaI CCWGG 4 cut(s) 364, 399, 1067, 1347
MvnI CGCG 1 cut(s) 206
MwoI GCNNNNNNNGC 3 cut(s) 113, 383, 596
NarI GGCGCC 1 cut(s) 1529
NciI CCSGG 1 cut(s) 1453
NdeII GATC 8 cut(s) 265, 295, 623, 661, 1241, 1402, 1422, 1545
NlaIII CATG 6 cut(s) 49, 302, 668, 913, 958, 1241
NlaIV GGNNCC 3 cut(s) 356, 625, 1530
NmuCI GTSAC 2 cut(s) 535, 1539
PagI TCATGA 2 cut(s) 298, 954
PcsI WCGNNNNNNNCGW 2 cut(s) 981, 1541
PfeI GAWTC 4 cut(s) 327, 847, 1144, 1441
PfoI TCCNGGA 2 cut(s) 397, 1345
PkrI GCNGC 8 cut(s) 7, 10, 37, 205, 338, 385, 765, 824
PluTI GGCGCC 1 cut(s) 1532
PmaCI CACGTG 1 cut(s) 1373
PmlI CACGTG 1 cut(s) 1373
Ppu21I YACGTR 2 cut(s) 1051, 1373
PshAI GACNNNNGTC 1 cut(s) 1469
PsiI TTATAA 1 cut(s) 17
Psp6I CCWGG 4 cut(s) 362, 397, 1065, 1345
PspCI CACGTG 1 cut(s) 1373
PspEI GGTNACC 1 cut(s) 1539
PspGI CCWGG 4 cut(s) 362, 397, 1065, 1345
PspN4I GGNNCC 3 cut(s) 356, 625, 1530
PspPI GGNCC 2 cut(s) 201, 1043
PstNI CAGNNNCTG 1 cut(s) 686
PsuI RGATCY 1 cut(s) 623
RsaI GTAC 3 cut(s) 210, 705, 1037
RsaNI GTAC 3 cut(s) 209, 704, 1036
RseI CAYNNNNRTG 1 cut(s) 561
SacII CCGCGG 1 cut(s) 207
SaqAI TTAA 1 cut(s) 786
SatI GCNGC 8 cut(s) 6, 9, 36, 204, 337, 384, 764, 823
Sau3AI GATC 8 cut(s) 265, 295, 623, 661, 1241, 1402, 1422, 1545
Sau96I GGNCC 2 cut(s) 201, 1043
ScrFI CCNGG 5 cut(s) 364, 399, 1067, 1347, 1453
SduI GDGCHC 1 cut(s) 954
SfaNI GCATC 5 cut(s) 47, 166, 229, 334, 924
SfoI GGCGCC 1 cut(s) 1530
Sfr303I CCGCGG 1 cut(s) 207
SgrBI CCGCGG 1 cut(s) 207
SinI GGWCC 1 cut(s) 1043
SmiMI CAYNNNNRTG 1 cut(s) 561
SmlI CTYRAG 1 cut(s) 1305
SmoI CTYRAG 1 cut(s) 1305
SsiI CCGC 9 cut(s) 8, 105, 204, 206, 337, 798, 932, 1025, 1089
SspDI GGCGCC 1 cut(s) 1528
SspMI CTAG 2 cut(s) 1151, 1181
StyD4I CCNGG 5 cut(s) 362, 397, 1065, 1345, 1451
StyI CCWWGG 2 cut(s) 1180, 1195
TaaI ACNGT 5 cut(s) 421, 560, 605, 682, 703
TaiI ACGT 4 cut(s) 166, 632, 1053, 1375
TaqI TCGA 3 cut(s) 857, 984, 1535
TatI WGTACW 1 cut(s) 703
TauI GCSGC 3 cut(s) 11, 206, 339
TfiI GAWTC 4 cut(s) 327, 847, 1144, 1441
Tru1I TTAA 1 cut(s) 786
Tru9I TTAA 1 cut(s) 786
TscAI CASTG 5 cut(s) 135, 384, 498, 546, 706
TseFI GTSAC 2 cut(s) 535, 1539
TseI GCWGC 5 cut(s) 5, 35, 383, 763, 822
Tsp45I GTSAC 2 cut(s) 535, 1539
TspDTI ATGAA 5 cut(s) 315, 382, 583, 715, 1287
TspGWI ACGGA 2 cut(s) 31, 990
TspRI CASTG 5 cut(s) 135, 384, 498, 546, 706
VpaK11BI GGWCC 1 cut(s) 1043
XapI RAATTY 6 cut(s) 19, 254, 674, 1248, 1354, 1407
XbaI TCTAGA 1 cut(s) 1150
XcmI CCANNNNNNNNNTGG 1 cut(s) 1377
XmaJI CCTAGG 1 cut(s) 1180
XspI CTAG 2 cut(s) 1151, 1181
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.