Rroxscaffold_5G00365400

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000950
Physical Location & Seq
Reverse (-)
46962245 .. 46964812
2568 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00365400.1

Sequence Viewer

Length: 1638 bp
ATGCGGCGAATCCAAGCTGCAATCTTCTATTGGCGTGGATTTGGAGACTTGGGTTTGGTTGAAAATGGAGTCTTTGGGGGCGAATTAGCAAAGTGGAGGAGGAATGTTTTCTTTGGCGTTAGAGGGATTTGGAAATCGTCGTCGTTGTCTATGAGGTTGAATGCGGAGTTGCAAGCCGAACAAGAGGTCGATGATCCGATGGGGCCTCGAGTTTATGGGCAAAAGTGTGACGATGATGACACGGGACTGGTTCTATTATGGAGTTCATCTAATTATGCGGGCCCGAAAGCCAATGCATCTGATAAAGTATTCTACGGAGCTGGCCTACTGTTAGCATTAGGCGAGGCTGGACGGCCGGCTGCTTTGGGTAAATTTCTCGAGGACCAATATTTAAGTGAAGCTGATCACAAGATCAAATTACCCGAAACAAAAAGTACAGAAGAAGAAGATCATATTTTGACCAGAAAAGAAGAAGATGACAAGCAAAAACGTGGGGAAACCCGTCAAACCGCTTTGTGGAGCCATCCCTGGTTCATAGGTGCGGCTGCACCTCTTGCCCTTGTAAATAGATCTTGGCCAACTCAACTACTGCTCTCAATGATCGCGGTGGGAGTTTCTTACTTATTGTTCTGGTATGGCGTCTTCGCGTATTCCAGAAATATTAGAAATGAATCAAATGATCCAGCGGTAGAAGATGCACCAAGAGATCCTGGACTTTGCCAAGCCATCAAAAGTCTCTCACCATCAGAAGACACAGCAAGCTGGAAGTTTTGGCTCAAACCCAATTCCAGAAAACGTTATTACAAAGAAATGCTAGTCATGTGCTTAGCCTTCATCGTGTACAGTCTGGTGGAGGCAACGGGAAGCACCTTCTTTTTCAATCAAATGAGTCTACTGGACTATCACATCGGGAACTTTGAAGTGCCCATCATATATTTCGTCATACTCCAATCCTTGTCAAGCTCCGTAATCTCATTTCTCTGGAGGTTACTAGTTCCAAATCAGTCAAAACGTGCTACGTTGGTCAGGATCGGCTGTGGTTTAGTTTTCTCTGTGTTATGTATGGTTGCTGCTTGGCAAGTTGAGATTCGTAGGAAAAACAAGATCAATACGGAATATATAGGTGATCCCAAACCGAAGGAAATTTCCATGAGCATTCTTTGGTTAATTCCACAATTCTCCCTGTTAGGGTTTATGAGAGGGTTAGCGGCAGATGGATTGATCGAATTTTTTGCGGATCGAGTTGATAGTAATGGTAAAAGAAAGGCTAGGTATTATGGATCCTACGCCAGTGAATTAGTTCTGGGTATTGGAACACTACTAACCGCCGTTAGTATTTTCGGGTTCAGACGAACTTGGTTGGTTAATGATATATACAAGAATCGTCTGGATAAGTACTACAGGGGACTAGTATTCATAGGTGTTGCTAACCTTTGCTATTACTTCTGTGTCGCCGTTTACTTTTATACCAAGGAGGATAACTCGCAAGTAAGTTGTGCCGCAGATGGAAATGAACAAAGTGAAATGCCGGACGAGGACAAAAAAAATTATCATCACGAGGTGTCCTCAGGTGGTGCGGAGACTCCGGACTTGGAAGAAATGAAGTCGAGTTTTGATTGTTGTAGTAGTTTCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

545

Amino Acids

61.71

Weight (kDa)

6.18

Isoelectric Point (pI)

44.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 263 - 415 2.2e-11 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 892
AccII CGCG 2 cut(s) 605, 647
AccIII TCCGGA 1 cut(s) 1585
AclI AACGTT 1 cut(s) 796
AclWI GGATC 8 cut(s) 188, 674, 701, 1037, 1121, 1245, 1275, 1288
AcoI YGGCCR 2 cut(s) 353, 575
AcsI RAATTY 3 cut(s) 371, 1143, 1226
AcyI GRCGYC 1 cut(s) 639
AdeI CACNNNGTG 1 cut(s) 1561
AfaI GTAC 3 cut(s) 436, 842, 1397
AfiI CCNNNNNNNGG 2 cut(s) 516, 1188
AgsI TTSAA 4 cut(s) 62, 160, 880, 920
AhlI ACTAGT 2 cut(s) 991, 1408
AjnI CCWGG 2 cut(s) 527, 709
AluBI AGCT 5 cut(s) 17, 320, 401, 762, 963
AluI AGCT 5 cut(s) 17, 320, 401, 762, 963
Alw26I GTCTC 3 cut(s) 39, 740, 1574
AlwI GGATC 8 cut(s) 188, 674, 701, 1037, 1121, 1245, 1275, 1288
Ama87I CYCGRG 2 cut(s) 207, 377
Aor13HI TCCGGA 1 cut(s) 1585
AoxI GGCC 5 cut(s) 203, 280, 322, 353, 575
ApaI GGGCCC 1 cut(s) 284
ApeKI GCWGC 4 cut(s) 17, 359, 545, 1070
ApoI RAATTY 3 cut(s) 371, 1143, 1226
ArsI GACNNNNNNTTYG 2 cut(s) 171, 203
Asp700I GAANNNNTTC 2 cut(s) 107, 1299
AspS9I GGNCC 4 cut(s) 203, 280, 281, 382
AsuHPI GGTGA 2 cut(s) 732, 1136
AvaI CYCGRG 2 cut(s) 207, 377
AvaII GGWCC 1 cut(s) 382
AxyI CCTNAGG 1 cut(s) 1567
BaeGI GKGCMC 2 cut(s) 284, 927
BalI TGGCCA 1 cut(s) 577
BamHI GGATCC 1 cut(s) 1280
BanII GRGCYC 1 cut(s) 284
BarI GAAGNNNNNNTAC 2 cut(s) 626, 658
BauI CACGAG 1 cut(s) 1556
BbsI GAAGAC 2 cut(s) 634, 756
BbvI GCAGC 4 cut(s) 4, 346, 532, 1057
BccI CCATC 7 cut(s) 193, 531, 734, 751, 935, 1208, 1499
BceAI ACGGC 3 cut(s) 368, 1313, 1439
BcgI CGANNNNNNTGC 2 cut(s) 1214, 1248
BciT130I CCWGG 2 cut(s) 529, 711
BclI TGATCA 1 cut(s) 403
BcoDI GTCTC 3 cut(s) 39, 740, 1574
BcuI ACTAGT 2 cut(s) 991, 1408
BfaI CTAG 4 cut(s) 815, 992, 1269, 1409
BfmI CTRYAG 1 cut(s) 1399
BglII AGATCT 1 cut(s) 569
BisI GCNGC 8 cut(s) 5, 18, 360, 543, 546, 1071, 1209, 1500
BlpI GCTNAGC 1 cut(s) 826
BlsI GCNGC 8 cut(s) 6, 19, 361, 544, 547, 1072, 1210, 1501
BmcAI AGTACT 1 cut(s) 1397
Bme1390I CCNGG 2 cut(s) 529, 711
Bme18I GGWCC 1 cut(s) 382
BmeT110I CYCGRG 2 cut(s) 207, 377
BmgT120I GGNCC 4 cut(s) 203, 280, 281, 382
BmiI GGNNCC 4 cut(s) 204, 282, 521, 1282
BmrFI CCNGG 2 cut(s) 529, 711
BmsI GCATC 2 cut(s) 305, 685
BpiI GAAGAC 2 cut(s) 634, 756
BplI GAGNNNNNCTC 4 cut(s) 1466, 1498, 1550, 1582
BpmI CTGGAG 1 cut(s) 1003
Bpu1102I GCTNAGC 1 cut(s) 826
BsaHI GRCGYC 1 cut(s) 639
BsaJI CCNNGG 2 cut(s) 527, 1470
BsaWI WCCGGW 1 cut(s) 1585
Bsc4I CCNNNNNNNGG 2 cut(s) 516, 1188
Bse118I RCCGGY 1 cut(s) 355
Bse1I ACTGG 3 cut(s) 252, 900, 1290
Bse21I CCTNAGG 1 cut(s) 1567
BseAI TCCGGA 1 cut(s) 1585
BseBI CCWGG 2 cut(s) 529, 711
BseDI CCNNGG 2 cut(s) 527, 1470
BseGI GGATG 1 cut(s) 523
BseLI CCNNNNNNNGG 2 cut(s) 516, 1188
BseMII CTCAG 1 cut(s) 1581
BseNI ACTGG 3 cut(s) 252, 900, 1290
BseRI GAGGAG 1 cut(s) 112
BseSI GKGCMC 2 cut(s) 284, 927
BseX3I CGGCCG 1 cut(s) 353
BseXI GCAGC 4 cut(s) 4, 346, 532, 1057
BsgI GTGCAG 1 cut(s) 531
Bsh1236I CGCG 2 cut(s) 605, 647
Bsh1285I CGRYCG 1 cut(s) 356
BshFI GGCC 5 cut(s) 205, 282, 324, 355, 577
BsiEI CGRYCG 1 cut(s) 356
BsiHKCI CYCGRG 2 cut(s) 207, 377
BsiSI CCGG 3 cut(s) 356, 1529, 1586
BslFI GGGAC 2 cut(s) 258, 1419
BslI CCNNNNNNNGG 2 cut(s) 516, 1188
BsmAI GTCTC 3 cut(s) 39, 740, 1574
BsmFI GGGAC 2 cut(s) 258, 1419
BsmI GAATGC 2 cut(s) 166, 1155
BsnI GGCC 5 cut(s) 205, 282, 324, 355, 577
BsoBI CYCGRG 2 cut(s) 207, 377
Bsp120I GGGCCC 1 cut(s) 280
Bsp1286I GDGCHC 2 cut(s) 284, 927
Bsp13I TCCGGA 1 cut(s) 1585
Bsp1407I TGTACA 1 cut(s) 840
Bsp1720I GCTNAGC 1 cut(s) 826
BspANI GGCC 5 cut(s) 205, 282, 324, 355, 577
BspCNI CTCAG 1 cut(s) 1580
BspEI TCCGGA 1 cut(s) 1585
BspFNI CGCG 2 cut(s) 605, 647
BspLI GGNNCC 4 cut(s) 204, 282, 521, 1282
BspPI GGATC 8 cut(s) 188, 674, 701, 1037, 1121, 1245, 1275, 1288
BsrFI RCCGGY 1 cut(s) 355
BsrGI TGTACA 1 cut(s) 840
BsrI ACTGG 3 cut(s) 252, 900, 1290
BssAI RCCGGY 1 cut(s) 355
BssECI CCNNGG 2 cut(s) 527, 1470
BssNI GRCGYC 1 cut(s) 639
BssSI CACGAG 1 cut(s) 1556
BssT1I CCWWGG 1 cut(s) 1470
Bst2BI CACGAG 1 cut(s) 1556
Bst2UI CCWGG 2 cut(s) 529, 711
Bst4CI ACNGT 2 cut(s) 330, 845
BstACI GRCGYC 1 cut(s) 639
BstAPI GCANNNNNTGC 1 cut(s) 554
BstAUI TGTACA 1 cut(s) 840
BstC8I GCNNGC 5 cut(s) 174, 280, 322, 357, 760
BstDEI CTNAG 2 cut(s) 826, 1567
BstF5I GGATG 1 cut(s) 523
BstFNI CGCG 2 cut(s) 605, 647
BstMAI GTCTC 3 cut(s) 39, 740, 1574
BstMCI CGRYCG 1 cut(s) 356
BstMWI GCNNNNNNNGC 1 cut(s) 554
BstNI CCWGG 2 cut(s) 529, 711
BstSCI CCNGG 2 cut(s) 527, 709
BstSFI CTRYAG 1 cut(s) 1399
BstSLI GKGCMC 2 cut(s) 284, 927
BstUI CGCG 2 cut(s) 605, 647
BstV1I GCAGC 4 cut(s) 4, 346, 532, 1057
BstV2I GAAGAC 2 cut(s) 634, 756
BstX2I RGATCY 3 cut(s) 569, 706, 1280
BstYI RGATCY 3 cut(s) 569, 706, 1280
BstZI CGGCCG 1 cut(s) 353
Bsu36I CCTNAGG 1 cut(s) 1567
BsuRI GGCC 5 cut(s) 205, 282, 324, 355, 577
BtsCI GGATG 1 cut(s) 523
BtsIMutI CAGTG 1 cut(s) 1297
Cac8I GCNNGC 5 cut(s) 174, 280, 322, 357, 760
Cfr10I RCCGGY 1 cut(s) 355
Cfr13I GGNCC 4 cut(s) 203, 280, 281, 382
CseI GACGC 1 cut(s) 628
Csp6I GTAC 3 cut(s) 435, 841, 1396
CviAII CATG 2 cut(s) 820, 1150
CviQI GTAC 3 cut(s) 435, 841, 1396
DdeI CTNAG 2 cut(s) 826, 1567
DraIII CACNNNGTG 1 cut(s) 1561
EaeI YGGCCR 2 cut(s) 353, 575
EagI CGGCCG 1 cut(s) 353
EclXI CGGCCG 1 cut(s) 353
Eco130I CCWWGG 1 cut(s) 1470
Eco24I GRGCYC 1 cut(s) 284
Eco47I GGWCC 1 cut(s) 382
Eco52I CGGCCG 1 cut(s) 353
Eco81I CCTNAGG 1 cut(s) 1567
Eco88I CYCGRG 2 cut(s) 207, 377
EcoO109I RGGNCCY 1 cut(s) 203
EcoRII CCWGG 2 cut(s) 527, 709
EcoT14I CCWWGG 1 cut(s) 1470
EcoT22I ATGCAT 1 cut(s) 298
EcoT38I GRGCYC 1 cut(s) 284
ErhI CCWWGG 1 cut(s) 1470
FaeI CATG 2 cut(s) 823, 1153
FaqI GGGAC 2 cut(s) 258, 1419
FatI CATG 2 cut(s) 819, 1149
FauI CCCGC 1 cut(s) 271
FbaI TGATCA 1 cut(s) 403
FblI GTMKAC 1 cut(s) 892
Fnu4HI GCNGC 8 cut(s) 5, 18, 360, 543, 546, 1071, 1209, 1500
FokI GGATG 1 cut(s) 510
FriOI GRGCYC 1 cut(s) 284
Fsp4HI GCNGC 8 cut(s) 5, 18, 360, 543, 546, 1071, 1209, 1500
FspBI CTAG 4 cut(s) 815, 992, 1269, 1409
GluI GCNGC 8 cut(s) 5, 18, 360, 543, 546, 1071, 1209, 1500
GsuI CTGGAG 1 cut(s) 1003
HaeIII GGCC 5 cut(s) 205, 282, 324, 355, 577
HapII CCGG 3 cut(s) 356, 1529, 1586
HgaI GACGC 1 cut(s) 628
Hin1I GRCGYC 1 cut(s) 639
Hin1II CATG 2 cut(s) 823, 1153
HinfI GANTC 7 cut(s) 9, 69, 671, 889, 1087, 1381, 1582
HpaII CCGG 3 cut(s) 356, 1529, 1586
HphI GGTGA 2 cut(s) 732, 1136
Hpy166II GTNNAC 3 cut(s) 841, 893, 1459
Hpy188I TCNGA 4 cut(s) 198, 301, 748, 1349
Hpy188III TCNNGA 9 cut(s) 377, 654, 789, 910, 982, 1027, 1388, 1556, 1586
Hpy8I GTNNAC 3 cut(s) 841, 893, 1459
Hpy99I CGWCG 2 cut(s) 142, 145
HpyAV CCTTC 3 cut(s) 841, 880, 1132
HpyCH4III ACNGT 2 cut(s) 330, 845
HpyCH4IV ACGT 4 cut(s) 490, 796, 1012, 1019
HpyCH4V TGCA 5 cut(s) 20, 172, 296, 548, 698
HpyF10VI GCNNNNNNNGC 1 cut(s) 554
HpyF3I CTNAG 2 cut(s) 826, 1567
HpySE526I ACGT 4 cut(s) 490, 796, 1012, 1019
Hsp92I GRCGYC 1 cut(s) 639
Hsp92II CATG 2 cut(s) 823, 1153
Kpn2I TCCGGA 1 cut(s) 1585
KroI GCCGGC 1 cut(s) 355
KroNI GCCGGC 1 cut(s) 357
Ksp22I TGATCA 1 cut(s) 403
LmnI GCTCC 3 cut(s) 317, 519, 968
Lsp1109I GCAGC 4 cut(s) 4, 346, 532, 1057
LweI GCATC 2 cut(s) 305, 685
MaeI CTAG 4 cut(s) 815, 992, 1269, 1409
MaeII ACGT 4 cut(s) 490, 796, 1012, 1019
MaeIII GTNAC 2 cut(s) 227, 987
MflI RGATCY 3 cut(s) 569, 706, 1280
MhlI GDGCHC 2 cut(s) 284, 927
MlsI TGGCCA 1 cut(s) 577
MluNI TGGCCA 1 cut(s) 577
MlyI GAGTC 3 cut(s) 78, 898, 1576
Mox20I TGGCCA 1 cut(s) 577
Mph1103I ATGCAT 1 cut(s) 298
MroI TCCGGA 1 cut(s) 1585
MroNI GCCGGC 1 cut(s) 355
MroXI GAANNNNTTC 2 cut(s) 107, 1299
MscI TGGCCA 1 cut(s) 577
MseI TTAA 3 cut(s) 392, 1166, 1365
Msp20I TGGCCA 1 cut(s) 577
MspA1I CMGCKG 1 cut(s) 686
MspI CCGG 3 cut(s) 356, 1529, 1586
MspR9I CCNGG 2 cut(s) 529, 711
Mva1269I GAATGC 2 cut(s) 166, 1155
MvaI CCWGG 2 cut(s) 529, 711
MvnI CGCG 2 cut(s) 605, 647
MwoI GCNNNNNNNGC 1 cut(s) 554
NaeI GCCGGC 1 cut(s) 357
NgoMIV GCCGGC 1 cut(s) 355
NlaIII CATG 2 cut(s) 823, 1153
NlaIV GGNNCC 4 cut(s) 204, 282, 521, 1282
NmuCI GTSAC 1 cut(s) 227
NsiI ATGCAT 1 cut(s) 298
PaeR7I CTCGAG 2 cut(s) 207, 377
PctI GAATGC 2 cut(s) 166, 1155
PdiI GCCGGC 1 cut(s) 357
PdmI GAANNNNTTC 2 cut(s) 107, 1299
PfeI GAWTC 4 cut(s) 9, 671, 1087, 1381
PfoI TCCNGGA 1 cut(s) 709
PkrI GCNGC 8 cut(s) 6, 19, 361, 544, 547, 1072, 1210, 1501
PleI GAGTC 3 cut(s) 77, 897, 1576
PpsI GAGTC 3 cut(s) 77, 897, 1576
Psp1406I AACGTT 1 cut(s) 796
Psp6I CCWGG 2 cut(s) 527, 709
PspGI CCWGG 2 cut(s) 527, 709
PspN4I GGNNCC 4 cut(s) 204, 282, 521, 1282
PspOMI GGGCCC 1 cut(s) 280
PspPI GGNCC 4 cut(s) 203, 280, 281, 382
PspXI VCTCGAGB 1 cut(s) 207
PsuI RGATCY 3 cut(s) 569, 706, 1280
RsaI GTAC 3 cut(s) 436, 842, 1397
RsaNI GTAC 3 cut(s) 435, 841, 1396
SaqAI TTAA 3 cut(s) 392, 1166, 1365
SatI GCNGC 8 cut(s) 5, 18, 360, 543, 546, 1071, 1209, 1500
Sau96I GGNCC 4 cut(s) 203, 280, 281, 382
ScaI AGTACT 1 cut(s) 1397
SchI GAGTC 3 cut(s) 78, 898, 1576
ScrFI CCNGG 2 cut(s) 529, 711
SduI GDGCHC 2 cut(s) 284, 927
SfaNI GCATC 2 cut(s) 305, 685
SfcI CTRYAG 1 cut(s) 1399
Sfr274I CTCGAG 2 cut(s) 207, 377
SinI GGWCC 1 cut(s) 382
SlaI CTCGAG 2 cut(s) 207, 377
SmlI CTYRAG 2 cut(s) 207, 377
SmoI CTYRAG 2 cut(s) 207, 377
SpeI ACTAGT 2 cut(s) 991, 1408
SspI AATATT 2 cut(s) 389, 661
SspMI CTAG 4 cut(s) 815, 992, 1269, 1409
StyD4I CCNGG 2 cut(s) 527, 709
StyI CCWWGG 1 cut(s) 1470
TaaI ACNGT 2 cut(s) 330, 845
TaiI ACGT 4 cut(s) 493, 799, 1015, 1022
TaqI TCGA 6 cut(s) 189, 208, 378, 1224, 1240, 1607
TatI WGTACW 3 cut(s) 434, 840, 1395
TauI GCSGC 4 cut(s) 7, 545, 1211, 1502
TfiI GAWTC 4 cut(s) 9, 671, 1087, 1381
Tru1I TTAA 3 cut(s) 392, 1166, 1365
Tru9I TTAA 3 cut(s) 392, 1166, 1365
TscAI CASTG 1 cut(s) 1297
TseFI GTSAC 1 cut(s) 227
TseI GCWGC 4 cut(s) 17, 359, 545, 1070
Tsp45I GTSAC 1 cut(s) 227
TspDTI ATGAA 7 cut(s) 255, 523, 684, 823, 1405, 1527, 1616
TspGWI ACGGA 3 cut(s) 330, 955, 1127
TspRI CASTG 1 cut(s) 1297
VpaK11BI GGWCC 1 cut(s) 382
XapI RAATTY 3 cut(s) 371, 1143, 1226
XhoI CTCGAG 2 cut(s) 207, 377
XmiI GTMKAC 1 cut(s) 892
XmnI GAANNNNTTC 2 cut(s) 107, 1299
XspI CTAG 4 cut(s) 815, 992, 1269, 1409
ZrmI AGTACT 1 cut(s) 1397
Zsp2I ATGCAT 1 cut(s) 298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.