MD13G1165300.v1.1

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0000950
Physical Location & Seq
Forward (+)
13143724 .. 13148582
4859 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1165300.v1.1.491

Sequence Viewer

Length: 1734 bp
ATGCTGCCATATAGTGAGAAAGTGGCTCGGAAACCACTTCTACTTCTCCAAGGCTACTACATGCATTCGAGCCACAGCTTCGTAAATCATGCAATAGTGGGTATCTTGGTAACGTACTTGACGGACGTATGGAGTACCGAACACTTTGAAATGGCTGCATTAGTTACCAATCTACAAGATGGGTTTACAGCCGTCACGGTTATTGGTCTTGCTCATATATCAGACACACACAAGAGTCGTTTTAAAATGATTGTCTCCACAAATGCTGCTTATTTTCTTGGACTTCTTCTATTATGGATTTGTTCTAAGTTCACGAGTGCACAAACTTCGGCTAAGGTATACTATGGAGCAGCCGTATTGTTAGTATTAGGCGAAGCTGGTCGATCGGCTACTTTGCAGGAATTTCTTGACGATCAATACTTCAGTGAACAGAAACAAACACCATCCTCAGATGAAAATTACTTAATACGTCTGGAAACTCGAAAAAAAACTTTGTGGTCCCATCCCTGGTTTTTAGGTGCATTCATATCTATTTTCCTTTCAACCGTGTCTTGGACACGTACCTTTTTTATTTCAGCAACTTTAGTGGGAGCTTCTTATCTAGTGTTCTTTCTTGGATACTCCTGCTACTTCGTACGAAGTAGAATCGATGAATCTAATTGGCGATCTCGTCTTAAATATATTGAAAAATTAGCCATCCTCTATCCAGTAGAACAACAAAGAGGAGGAGAGATTTGCATGGTCACCAGATTTGCCCACTCGTCGCCGTTGGGAGGACAAGAAGAACAGAGAAAAAAGTTATGGCTACCCATAAAACTCAAGGGGGGAAAGTGGTTTTTCATAGAAGTGATAGCTATGTGGTCGACCTTTTTAGCATACAGTTTGGTGGAGGCAACAGGTAGTACGTTCTTTTTCGAACAAATGAGTAACTTGGATAATAAATTTAAATCATTGAAGGTAGAGCCAATTTATTTCGAAGTATTGGGTTCCTTCTCGTGTTTCTTGGTATCGTTTCTGTATGAGTTACTAGTTCCGAAGAAGTGGCCAAACGCTATGCTGGTGAGAATTGGCTTGGGATTGGGTTGTTCCGTGCTATGTTGTGCTGCTGCATGGCAGGTTGAGAGAAAAAGGTTGAAGTTAGTTAGGAATGAGCGGTGGCATGAATATGATACTTCTGAGGCTACTTCTATGAGCATTTTGTGGCTGGTTCCGCAGTTTTTCCTGCTGGGACTAATGCAAGGGCTGGCCAAGGATGGACTGATTGATTTCCTTGCTGATCGAATTGCTAATATTGATAGACTAAGGGCTTGGTATTATGCATCCCACTTAACTGACTTCGCACTTGGCGTTGGAAAAATAACTACAGCCATTAGCATTTTGGTGTTCCGGCGAATTTGGTTCGATGACAGCATAAATAGGAGTCACGTGGACAAGTTTTACAAATGGTTAACATTCCTAAGTCTCATTAACGTAATTTATTACGTGTGCATCTCCTTTTACTTGTATGGCAACGAGGAATCACAAAATTCAGGTGACAGAGAATACGAAAAAATTACTGAATTAGCAGAAGGAGTGGAGGGGTGGAATGTTGATGATGAGAATGAGGTCTTAGACCCTCCTGTAGGTGGTGCTGAAAATGAAGATGAACAAACTACGATGGAAGAAGTACCGGTGCTCAATGACGATGATGAGATCCCTTCGTGGTCGTATGACCTTCACAGGTGCACAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

578

Amino Acids

66.3

Weight (kDa)

5.74

Isoelectric Point (pI)

42.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 81 - 423 1.9e-12 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 1105
AccBSI CCGCTC 1 cut(s) 1153
AccI GTMKAC 2 cut(s) 339, 863
AciI CCGC 2 cut(s) 1153, 1211
AclWI GGATC 1 cut(s) 1687
AcoI YGGCCR 2 cut(s) 1043, 1245
AcsI RAATTY 4 cut(s) 401, 941, 1392, 1525
AcuI CTGAAG 1 cut(s) 406
AcvI CACGTG 1 cut(s) 1426
AdeI CACNNNGTG 1 cut(s) 1731
AfaI GTAC 6 cut(s) 116, 136, 562, 636, 904, 1668
AfiI CCNNNNNNNGG 5 cut(s) 507, 552, 773, 1622, 1625
AflIII ACRYGT 2 cut(s) 557, 1482
AgeI ACCGGT 1 cut(s) 1669
AgsI TTSAA 5 cut(s) 149, 543, 686, 955, 1135
AhlI ACTAGT 1 cut(s) 1027
AjnI CCWGG 1 cut(s) 506
AluBI AGCT 4 cut(s) 78, 377, 593, 854
AluI AGCT 4 cut(s) 78, 377, 593, 854
Alw21I GWGCWC 3 cut(s) 322, 1677, 1727
Alw26I GTCTC 2 cut(s) 259, 1466
Alw44I GTGCAC 2 cut(s) 318, 1723
AlwI GGATC 1 cut(s) 1687
AoxI GGCC 2 cut(s) 1043, 1245
ApaLI GTGCAC 2 cut(s) 318, 1723
ApeKI GCWGC 6 cut(s) 4, 155, 266, 350, 1103, 1106
ApoI RAATTY 4 cut(s) 401, 941, 1392, 1525
AsiGI ACCGGT 1 cut(s) 1669
AspS9I GGNCC 1 cut(s) 498
AsuHPI GGTGA 3 cut(s) 736, 1072, 1544
AsuII TTCGAA 2 cut(s) 915, 975
AvaII GGWCC 1 cut(s) 498
BaeGI GKGCMC 2 cut(s) 322, 1727
BalI TGGCCA 2 cut(s) 1045, 1247
BauI CACGAG 2 cut(s) 313, 994
BbrPI CACGTG 1 cut(s) 1426
Bbv12I GWGCWC 3 cut(s) 322, 1677, 1727
BbvI GCAGC 5 cut(s) 142, 253, 362, 1090, 1093
BccI CCATC 6 cut(s) 173, 451, 510, 704, 1247, 1651
BceAI ACGGC 3 cut(s) 176, 338, 751
BcgI CGANNNNNNTGC 2 cut(s) 309, 343
BciT130I CCWGG 1 cut(s) 508
BciVI GTATCC 1 cut(s) 611
BcoDI GTCTC 2 cut(s) 259, 1466
BcuI ACTAGT 1 cut(s) 1027
BfaI CTAG 2 cut(s) 602, 1028
BfmI CTRYAG 2 cut(s) 1362, 1620
BfuAI ACCTGC 1 cut(s) 1105
BfuI GTATCC 1 cut(s) 611
BisI GCNGC 6 cut(s) 5, 156, 267, 351, 1104, 1107
BlsI GCNGC 6 cut(s) 6, 157, 268, 352, 1105, 1108
Bme1390I CCNGG 1 cut(s) 508
Bme18I GGWCC 1 cut(s) 498
BmgT120I GGNCC 1 cut(s) 498
BmiI GGNNCC 3 cut(s) 500, 988, 1209
BmrFI CCNGG 1 cut(s) 508
BmsI GCATC 2 cut(s) 1328, 1497
Bpu10I CCTNAGC 1 cut(s) 333
Bpu14I TTCGAA 2 cut(s) 915, 975
BpuEI CTTGAG 1 cut(s) 803
Bsa29I ATCGAT 1 cut(s) 648
BsaAI YACGTR 3 cut(s) 560, 1426, 1483
BsaJI CCNNGG 3 cut(s) 49, 506, 1248
BsaWI WCCGGW 1 cut(s) 1669
BsaXI ACNNNNNCTCC 2 cut(s) 339, 369
Bsc4I CCNNNNNNNGG 5 cut(s) 507, 552, 773, 1622, 1625
Bse118I RCCGGY 1 cut(s) 1669
Bse1I ACTGG 1 cut(s) 707
BseBI CCWGG 1 cut(s) 508
BseCI ATCGAT 1 cut(s) 648
BseDI CCNNGG 3 cut(s) 49, 506, 1248
BseGI GGATG 5 cut(s) 443, 502, 696, 1258, 1319
BseLI CCNNNNNNNGG 5 cut(s) 507, 552, 773, 1622, 1625
BseMII CTCAG 2 cut(s) 462, 1167
BseNI ACTGG 1 cut(s) 707
BseRI GAGGAG 2 cut(s) 738, 741
BseSI GKGCMC 2 cut(s) 322, 1727
BseXI GCAGC 5 cut(s) 142, 253, 362, 1090, 1093
BseYI CCCAGC 1 cut(s) 1225
Bsh1285I CGRYCG 1 cut(s) 386
BshFI GGCC 2 cut(s) 1045, 1247
BshTI ACCGGT 1 cut(s) 1669
BshVI ATCGAT 1 cut(s) 648
BsiEI CGRYCG 1 cut(s) 386
BsiHKAI GWGCWC 3 cut(s) 322, 1677, 1727
BsiSI CCGG 2 cut(s) 1387, 1670
BsiWI CGTACG 1 cut(s) 634
BslFI GGGAC 2 cut(s) 484, 1242
BslI CCNNNNNNNGG 5 cut(s) 507, 552, 773, 1622, 1625
BsmAI GTCTC 2 cut(s) 259, 1466
BsmFI GGGAC 2 cut(s) 484, 1242
BsmI GAATGC 2 cut(s) 64, 521
BsnI GGCC 2 cut(s) 1045, 1247
Bsp119I TTCGAA 2 cut(s) 915, 975
Bsp1286I GDGCHC 3 cut(s) 322, 1677, 1727
Bsp143I GATC 5 cut(s) 383, 412, 665, 1276, 1692
BspACI CCGC 2 cut(s) 1153, 1211
BspANI GGCC 2 cut(s) 1045, 1247
BspCNI CTCAG 2 cut(s) 461, 1168
BspDI ATCGAT 1 cut(s) 648
BspLI GGNNCC 3 cut(s) 500, 988, 1209
BspMI ACCTGC 1 cut(s) 1105
BspPI GGATC 1 cut(s) 1687
BspT104I TTCGAA 2 cut(s) 915, 975
BsrBI CCGCTC 1 cut(s) 1153
BsrFI RCCGGY 1 cut(s) 1669
BsrI ACTGG 1 cut(s) 707
BssAI RCCGGY 1 cut(s) 1669
BssECI CCNNGG 3 cut(s) 49, 506, 1248
BssMI GATC 5 cut(s) 383, 412, 665, 1276, 1692
BssNAI GTATAC 1 cut(s) 340
BssSI CACGAG 2 cut(s) 313, 994
BssT1I CCWWGG 2 cut(s) 49, 1248
Bst1107I GTATAC 1 cut(s) 340
Bst2BI CACGAG 2 cut(s) 313, 994
Bst2UI CCWGG 1 cut(s) 508
Bst4CI ACNGT 3 cut(s) 199, 547, 881
BstBAI YACGTR 3 cut(s) 560, 1426, 1483
BstBI TTCGAA 2 cut(s) 915, 975
BstC8I GCNNGC 1 cut(s) 1245
BstDEI CTNAG 7 cut(s) 306, 333, 448, 1176, 1301, 1457, 1609
BstEII GGTNACC 1 cut(s) 742
BstENI CCTNNNNNAGG 1 cut(s) 1620
BstF5I GGATG 5 cut(s) 443, 502, 696, 1258, 1319
BstKTI GATC 5 cut(s) 386, 415, 668, 1279, 1695
BstMAI GTCTC 2 cut(s) 259, 1466
BstMBI GATC 5 cut(s) 383, 412, 665, 1276, 1692
BstMCI CGRYCG 1 cut(s) 386
BstMWI GCNNNNNNNGC 1 cut(s) 1210
BstNI CCWGG 1 cut(s) 508
BstNSI RCATGY 1 cut(s) 64
BstPI GGTNACC 1 cut(s) 742
BstSCI CCNGG 1 cut(s) 506
BstSFI CTRYAG 2 cut(s) 1362, 1620
BstSLI GKGCMC 2 cut(s) 322, 1727
BstV1I GCAGC 5 cut(s) 142, 253, 362, 1090, 1093
BstX2I RGATCY 1 cut(s) 1692
BstYI RGATCY 1 cut(s) 1692
BstZ17I GTATAC 1 cut(s) 340
Bsu15I ATCGAT 1 cut(s) 648
BsuI GTATCC 1 cut(s) 611
BsuRI GGCC 2 cut(s) 1045, 1247
BsuTUI ATCGAT 1 cut(s) 648
BtsCI GGATG 5 cut(s) 443, 502, 696, 1258, 1319
BtsIMutI CAGTG 1 cut(s) 430
BveI ACCTGC 1 cut(s) 1105
Cac8I GCNNGC 1 cut(s) 1245
Cfr10I RCCGGY 1 cut(s) 1669
Cfr13I GGNCC 1 cut(s) 498
ClaI ATCGAT 1 cut(s) 648
Csp6I GTAC 6 cut(s) 115, 135, 561, 635, 903, 1667
CspAI ACCGGT 1 cut(s) 1669
CspCI CAANNNNNGTGG 2 cut(s) 567, 602
CviAII CATG 5 cut(s) 61, 89, 739, 1110, 1160
CviQI GTAC 6 cut(s) 115, 135, 561, 635, 903, 1667
DdeI CTNAG 7 cut(s) 306, 333, 448, 1176, 1301, 1457, 1609
DpnI GATC 5 cut(s) 385, 414, 667, 1278, 1694
DpnII GATC 5 cut(s) 383, 412, 665, 1276, 1692
DraI TTTAAA 2 cut(s) 244, 946
DraIII CACNNNGTG 1 cut(s) 1731
EaeI YGGCCR 2 cut(s) 1043, 1245
Eco130I CCWWGG 2 cut(s) 49, 1248
Eco47I GGWCC 1 cut(s) 498
Eco57I CTGAAG 1 cut(s) 406
Eco72I CACGTG 1 cut(s) 1426
Eco91I GGTNACC 1 cut(s) 742
EcoNI CCTNNNNNAGG 1 cut(s) 1620
EcoO65I GGTNACC 1 cut(s) 742
EcoRII CCWGG 1 cut(s) 506
EcoT14I CCWWGG 2 cut(s) 49, 1248
EcoT22I ATGCAT 2 cut(s) 66, 1321
ErhI CCWWGG 2 cut(s) 49, 1248
FaeI CATG 5 cut(s) 64, 92, 742, 1113, 1163
FaqI GGGAC 2 cut(s) 484, 1242
FatI CATG 5 cut(s) 60, 88, 738, 1109, 1159
FblI GTMKAC 2 cut(s) 339, 863
Fnu4HI GCNGC 6 cut(s) 5, 156, 267, 351, 1104, 1107
FokI GGATG 5 cut(s) 430, 489, 683, 1265, 1306
Fsp4HI GCNGC 6 cut(s) 5, 156, 267, 351, 1104, 1107
FspBI CTAG 2 cut(s) 602, 1028
GluI GCNGC 6 cut(s) 5, 156, 267, 351, 1104, 1107
GsaI CCCAGC 1 cut(s) 1229
HaeIII GGCC 2 cut(s) 1045, 1247
HapII CCGG 2 cut(s) 1387, 1670
Hin1II CATG 5 cut(s) 64, 92, 742, 1113, 1163
HincII GTYRAC 2 cut(s) 864, 1449
HindII GTYRAC 2 cut(s) 864, 1449
HinfI GANTC 5 cut(s) 235, 645, 653, 1420, 1517
HpaI GTTAAC 1 cut(s) 1449
HpaII CCGG 2 cut(s) 1387, 1670
HphI GGTGA 3 cut(s) 736, 1072, 1544
Hpy166II GTNNAC 9 cut(s) 186, 312, 320, 340, 428, 864, 1429, 1449, 1725
Hpy188I TCNGA 5 cut(s) 30, 223, 451, 1035, 1177
Hpy188III TCNNGA 3 cut(s) 313, 407, 473
Hpy8I GTNNAC 9 cut(s) 186, 312, 320, 340, 428, 864, 1429, 1449, 1725
Hpy99I CGWCG 1 cut(s) 766
HpyAV CCTTC 5 cut(s) 949, 1000, 1562, 1707, 1724
HpyCH4III ACNGT 3 cut(s) 199, 547, 881
HpyCH4IV ACGT 8 cut(s) 113, 126, 469, 559, 905, 1425, 1470, 1482
HpyF10VI GCNNNNNNNGC 1 cut(s) 1210
HpyF3I CTNAG 7 cut(s) 306, 333, 448, 1176, 1301, 1457, 1609
HpySE526I ACGT 8 cut(s) 113, 126, 469, 559, 905, 1425, 1470, 1482
Hsp92II CATG 5 cut(s) 64, 92, 742, 1113, 1163
KspAI GTTAAC 1 cut(s) 1449
Kzo9I GATC 5 cut(s) 383, 412, 665, 1276, 1692
LmnI GCTCC 2 cut(s) 347, 590
Lsp1109I GCAGC 5 cut(s) 142, 253, 362, 1090, 1093
LweI GCATC 2 cut(s) 1328, 1497
MaeI CTAG 2 cut(s) 602, 1028
MaeII ACGT 8 cut(s) 113, 126, 469, 559, 905, 1425, 1470, 1482
MaeIII GTNAC 8 cut(s) 109, 163, 193, 742, 926, 1023, 1421, 1532
MalI GATC 5 cut(s) 385, 414, 667, 1278, 1694
MbiI CCGCTC 1 cut(s) 1153
MboI GATC 5 cut(s) 383, 412, 665, 1276, 1692
MboII GAAGA 5 cut(s) 278, 794, 1048, 1652, 1673
MflI RGATCY 1 cut(s) 1692
MhlI GDGCHC 3 cut(s) 322, 1677, 1727
MlsI TGGCCA 2 cut(s) 1045, 1247
MluNI TGGCCA 2 cut(s) 1045, 1247
MlyI GAGTC 2 cut(s) 244, 1429
MmeI TCCRAC 1 cut(s) 1330
Mox20I TGGCCA 2 cut(s) 1045, 1247
Mph1103I ATGCAT 2 cut(s) 66, 1321
MscI TGGCCA 2 cut(s) 1045, 1247
MseI TTAA 7 cut(s) 243, 464, 675, 945, 1328, 1448, 1467
MslI CAYNNNNRTG 3 cut(s) 845, 1164, 1379
Msp20I TGGCCA 2 cut(s) 1045, 1247
MspI CCGG 2 cut(s) 1387, 1670
MspR9I CCNGG 1 cut(s) 508
Mva1269I GAATGC 2 cut(s) 64, 521
MvaI CCWGG 1 cut(s) 508
MwoI GCNNNNNNNGC 1 cut(s) 1210
NdeII GATC 5 cut(s) 383, 412, 665, 1276, 1692
NlaIII CATG 5 cut(s) 64, 92, 742, 1113, 1163
NlaIV GGNNCC 3 cut(s) 500, 988, 1209
NmuCI GTSAC 4 cut(s) 193, 742, 1421, 1532
NsiI ATGCAT 2 cut(s) 66, 1321
NspI RCATGY 1 cut(s) 64
NspV TTCGAA 2 cut(s) 915, 975
PcsI WCGNNNNNNNCGW 1 cut(s) 1344
PctI GAATGC 2 cut(s) 64, 521
PfeI GAWTC 3 cut(s) 645, 653, 1517
Pfl23II CGTACG 1 cut(s) 634
PinAI ACCGGT 1 cut(s) 1669
PkrI GCNGC 6 cut(s) 6, 157, 268, 352, 1105, 1108
Ple19I CGATCG 1 cut(s) 386
PleI GAGTC 2 cut(s) 243, 1428
PmaCI CACGTG 1 cut(s) 1426
PmlI CACGTG 1 cut(s) 1426
PpsI GAGTC 2 cut(s) 243, 1428
Ppu21I YACGTR 3 cut(s) 560, 1426, 1483
Psp6I CCWGG 1 cut(s) 506
PspCI CACGTG 1 cut(s) 1426
PspEI GGTNACC 1 cut(s) 742
PspFI CCCAGC 1 cut(s) 1225
PspGI CCWGG 1 cut(s) 506
PspLI CGTACG 1 cut(s) 634
PspN4I GGNNCC 3 cut(s) 500, 988, 1209
PspPI GGNCC 1 cut(s) 498
PsuI RGATCY 1 cut(s) 1692
PvuI CGATCG 1 cut(s) 386
RsaI GTAC 6 cut(s) 116, 136, 562, 636, 904, 1668
RsaNI GTAC 6 cut(s) 115, 135, 561, 635, 903, 1667
RseI CAYNNNNRTG 3 cut(s) 845, 1164, 1379
SalI GTCGAC 1 cut(s) 862
SaqAI TTAA 7 cut(s) 243, 464, 675, 945, 1328, 1448, 1467
SatI GCNGC 6 cut(s) 5, 156, 267, 351, 1104, 1107
Sau3AI GATC 5 cut(s) 383, 412, 665, 1276, 1692
Sau96I GGNCC 1 cut(s) 498
SchI GAGTC 2 cut(s) 244, 1429
ScrFI CCNGG 1 cut(s) 508
SduI GDGCHC 3 cut(s) 322, 1677, 1727
SfaNI GCATC 2 cut(s) 1328, 1497
SfcI CTRYAG 2 cut(s) 1362, 1620
SfuI TTCGAA 2 cut(s) 915, 975
SinI GGWCC 1 cut(s) 498
SmiI ATTTAAAT 1 cut(s) 946
SmiMI CAYNNNNRTG 3 cut(s) 845, 1164, 1379
SmlI CTYRAG 1 cut(s) 818
SmoI CTYRAG 1 cut(s) 818
SpeI ACTAGT 1 cut(s) 1027
SsiI CCGC 2 cut(s) 1153, 1211
SspI AATATT 1 cut(s) 1291
SspMI CTAG 2 cut(s) 602, 1028
StyD4I CCNGG 1 cut(s) 506
StyI CCWWGG 2 cut(s) 49, 1248
SwaI ATTTAAAT 1 cut(s) 946
TaaI ACNGT 3 cut(s) 199, 547, 881
TaiI ACGT 8 cut(s) 116, 129, 472, 562, 908, 1428, 1473, 1485
TaqI TCGA 9 cut(s) 68, 382, 481, 648, 863, 915, 975, 1279, 1401
TfiI GAWTC 3 cut(s) 645, 653, 1517
Tru1I TTAA 7 cut(s) 243, 464, 675, 945, 1328, 1448, 1467
Tru9I TTAA 7 cut(s) 243, 464, 675, 945, 1328, 1448, 1467
TscAI CASTG 1 cut(s) 430
TseFI GTSAC 4 cut(s) 193, 742, 1421, 1532
TseI GCWGC 6 cut(s) 4, 155, 266, 350, 1103, 1106
Tsp45I GTSAC 4 cut(s) 193, 742, 1421, 1532
TspDTI ATGAA 7 cut(s) 468, 514, 666, 829, 1176, 1653, 1659
TspGWI ACGGA 2 cut(s) 137, 1078
TspRI CASTG 1 cut(s) 430
VneI GTGCAC 2 cut(s) 318, 1723
VpaK11BI GGWCC 1 cut(s) 498
XagI CCTNNNNNAGG 1 cut(s) 1620
XapI RAATTY 4 cut(s) 401, 941, 1392, 1525
XceI RCATGY 1 cut(s) 64
XcmI CCANNNNNNNNNTGG 1 cut(s) 1375
XmiI GTMKAC 2 cut(s) 339, 863
XspI CTAG 2 cut(s) 602, 1028
Zsp2I ATGCAT 2 cut(s) 66, 1321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.