Rh4BG124300

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000950
Physical Location & Seq
Forward (+)
20783291 .. 20805603
22313 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG124300.1

Sequence Viewer

Length: 1305 bp
ATGGCTGCGGCACTTATAAATTTGATAGTTGGGGCAGCATCCGTCATGGTCATCGTCCTTGCTTATCTATCACACAGAATAGGTCATTTTAGAATGGCTGTCTCCGCAAGTTTTGCCTATACTCTTGCACTGGCTCTATTATGGATTTCAGCCAAGTTTATACGTCAGGGCGCAGATGCAGTAGATAATTTTTTCTATTGGGCCGCGGTACTATTGGCAATAGCTGGCGCTGGAATGGATGCTGTTATACCTAAATTTCTTGACGATCAATACACAAGTGAACAGAGAGAAAGAGATCATGAATACACAATAGAAGAAGAAGAACGATTCCAAAGCCGGAAAGATGCTTTGTGGAGCCATCCCTGGTTTTTCGGTGCAGTGGCATCTCTTTTCATTCCTGGAGAGGCACATTGGAAAACTGTCTTCCTGATTTCAATGATTGCAATGGGGGTTTGTTTTTTATTGTTCTGGTTTGGCAAATCCTTTTATTCAGTGTCATCTTCAGTTGAAACAGCACCAAGAGGGAAGTTTGGGGTCACACTGAAAGAAAATGGACACCGTTATGCAGAAATGTTGGCTATGAGCTTTGCCTTCATTGCCTACAGTCTGGTGGAGGCAACAGGATCCACGTTCTTTTTTGAACAAACAACTTTCTTGGATGATCATGTCGGAAAATTCACAGTTCCTGTCGTATATCTCACTGTACTTCAGTCTTTTTCAAAGTTCATAATCCCACATCTTTGGGGGCTACTAATTCCAAAGCAGCGAAGAAGTGTTGCATTGGTTAAAATTGGGTGCGGTTTGGTTCTTTCTGTTTTATGCTGTGTCATTGCTTGGCGAATTGAGATTCGTAGGTCGAGTAAAATCAAGGTGGACGTTGACCTTGAAGATTACAAACCAATTATTTCCATGAGCATCCTTTGGTTACTTCCACAATTCTCTCTGCTAGGGCTCATGAGAGGGTTAGCCGAAGACGGATTGGTCGAGTTTTTTGCAGAACAAGTGGTTGACGATGATAAACAAGTGGTGCTTAGGTACTATGGACCTCACGTATTTTCCTTCGTCCTGGGTATTGGAACACTACTGACCGCTGTTAGTATTTTGGGTTTTAGACGAACTTGGTTGGATGATGATATATATAAGAATCGTCTAGATAAGTATTACAGGGGATTAGTATTCCTAGGTGTTGCTAACCTTGGCTACTACTTGTGTATCGCCCTATACTTTTACACTAAGCATGGATCACAAATTTCTGCTGAAGAAACAAATGATGAAGAAACTGCAATAGATCTAGAGGATCCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

434

Amino Acids

49.04

Weight (kDa)

5.73

Isoelectric Point (pI)

31.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 180 - 343 6.1e-11 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 17
AasI GACNNNNNNGTC 1 cut(s) 980
AccII CGCG 1 cut(s) 206
AciI CCGC 6 cut(s) 8, 105, 204, 206, 798, 1089
AclWI GGATC 4 cut(s) 618, 631, 1249, 1292
AcsI RAATTY 4 cut(s) 19, 254, 674, 1248
AcuI CTGAAG 3 cut(s) 486, 692, 1278
AfaI GTAC 3 cut(s) 210, 705, 1037
AgsI TTSAA 5 cut(s) 435, 509, 641, 720, 887
AjnI CCWGG 3 cut(s) 362, 397, 1065
AluBI AGCT 2 cut(s) 224, 585
AluI AGCT 2 cut(s) 224, 585
Alw26I GTCTC 1 cut(s) 106
AlwI GGATC 4 cut(s) 618, 631, 1249, 1292
AlwNI CAGNNNCTG 1 cut(s) 686
AoxI GGCC 1 cut(s) 201
ApeKI GCWGC 3 cut(s) 5, 35, 763
ApoI RAATTY 4 cut(s) 19, 254, 674, 1248
AspA2I CCTAGG 1 cut(s) 1180
AspLEI GCGC 2 cut(s) 173, 230
AspS9I GGNCC 2 cut(s) 201, 1043
AvaII GGWCC 1 cut(s) 1043
AvrII CCTAGG 1 cut(s) 1180
BaeI ACNNNNGTAYC 2 cut(s) 1195, 1228
BamHI GGATCC 2 cut(s) 623, 1297
BanII GRGCYC 1 cut(s) 954
BbsI GAAGAC 2 cut(s) 415, 978
BbvI GCAGC 2 cut(s) 47, 775
BccI CCATC 1 cut(s) 366
BcgI CGANNNNNNTGC 2 cut(s) 974, 1008
BciT130I CCWGG 3 cut(s) 364, 399, 1067
BclI TGATCA 1 cut(s) 661
BcoDI GTCTC 1 cut(s) 106
BfaI CTAG 4 cut(s) 947, 1151, 1181, 1292
BfmI CTRYAG 1 cut(s) 601
BfoI RGCGCY 1 cut(s) 231
BglII AGATCT 1 cut(s) 1288
BisI GCNGC 5 cut(s) 6, 9, 36, 204, 764
BlnI CCTAGG 1 cut(s) 1180
BlsI GCNGC 5 cut(s) 7, 10, 37, 205, 765
Bme1390I CCNGG 3 cut(s) 364, 399, 1067
Bme18I GGWCC 1 cut(s) 1043
BmgT120I GGNCC 2 cut(s) 201, 1043
BmiI GGNNCC 3 cut(s) 356, 625, 1299
BmrFI CCNGG 3 cut(s) 364, 399, 1067
BmsI GCATC 6 cut(s) 47, 166, 229, 334, 392, 924
BpiI GAAGAC 2 cut(s) 415, 978
BpmI CTGGAG 1 cut(s) 420
Bpu10I CCTNAGC 1 cut(s) 1031
BsaAI YACGTR 1 cut(s) 1051
BsaJI CCNNGG 5 cut(s) 204, 362, 1066, 1180, 1195
Bse1I ACTGG 1 cut(s) 135
Bse3DI GCAATG 3 cut(s) 450, 594, 828
BseBI CCWGG 3 cut(s) 364, 399, 1067
BseDI CCNNGG 5 cut(s) 204, 362, 1066, 1180, 1195
BseGI GGATG 6 cut(s) 38, 244, 358, 664, 915, 1132
BseMI GCAATG 3 cut(s) 450, 594, 828
BseNI ACTGG 1 cut(s) 135
BseXI GCAGC 2 cut(s) 47, 775
BsgI GTGCAG 1 cut(s) 396
Bsh1236I CGCG 1 cut(s) 206
BshFI GGCC 1 cut(s) 203
BsiSI CCGG 1 cut(s) 337
BsmAI GTCTC 1 cut(s) 106
BsnI GGCC 1 cut(s) 203
Bsp1286I GDGCHC 1 cut(s) 954
Bsp143I GATC 7 cut(s) 265, 295, 623, 661, 1241, 1288, 1297
BspACI CCGC 6 cut(s) 8, 105, 204, 206, 798, 1089
BspANI GGCC 1 cut(s) 203
BspFNI CGCG 1 cut(s) 206
BspHI TCATGA 2 cut(s) 298, 954
BspLI GGNNCC 3 cut(s) 356, 625, 1299
BspPI GGATC 4 cut(s) 618, 631, 1249, 1292
BsrDI GCAATG 3 cut(s) 450, 594, 828
BsrI ACTGG 1 cut(s) 135
BssECI CCNNGG 5 cut(s) 204, 362, 1066, 1180, 1195
BssMI GATC 7 cut(s) 265, 295, 623, 661, 1241, 1288, 1297
BssT1I CCWWGG 2 cut(s) 1180, 1195
Bst2UI CCWGG 3 cut(s) 364, 399, 1067
Bst4CI ACNGT 5 cut(s) 421, 560, 605, 682, 703
BstAPI GCANNNNNTGC 1 cut(s) 113
BstBAI YACGTR 1 cut(s) 1051
BstC8I GCNNGC 1 cut(s) 226
BstDEI CTNAG 2 cut(s) 1031, 1233
BstDSI CCRYGG 1 cut(s) 204
BstF5I GGATG 6 cut(s) 38, 244, 358, 664, 915, 1132
BstFNI CGCG 1 cut(s) 206
BstH2I RGCGCY 1 cut(s) 231
BstHHI GCGC 2 cut(s) 173, 230
BstKTI GATC 7 cut(s) 268, 298, 626, 664, 1244, 1291, 1300
BstMAI GTCTC 1 cut(s) 106
BstMBI GATC 7 cut(s) 265, 295, 623, 661, 1241, 1288, 1297
BstMWI GCNNNNNNNGC 3 cut(s) 104, 113, 596
BstNI CCWGG 3 cut(s) 364, 399, 1067
BstSCI CCNGG 3 cut(s) 362, 397, 1065
BstSFI CTRYAG 1 cut(s) 601
BstUI CGCG 1 cut(s) 206
BstV1I GCAGC 2 cut(s) 47, 775
BstV2I GAAGAC 2 cut(s) 415, 978
BstX2I RGATCY 3 cut(s) 623, 1288, 1297
BstXI CCANNNNNNTGG 1 cut(s) 741
BstYI RGATCY 3 cut(s) 623, 1288, 1297
BsuRI GGCC 1 cut(s) 203
BtgI CCRYGG 1 cut(s) 204
BtsCI GGATG 6 cut(s) 38, 244, 358, 664, 915, 1132
BtsI GCAGTG 1 cut(s) 384
BtsIMutI CAGTG 5 cut(s) 128, 384, 498, 539, 699
Cac8I GCNNGC 1 cut(s) 226
CaiI CAGNNNCTG 1 cut(s) 686
CciI TCATGA 2 cut(s) 298, 954
CfoI GCGC 2 cut(s) 173, 230
Cfr13I GGNCC 2 cut(s) 201, 1043
Cfr42I CCGCGG 1 cut(s) 207
Csp6I GTAC 3 cut(s) 209, 704, 1036
CviAII CATG 6 cut(s) 46, 299, 665, 910, 955, 1238
CviQI GTAC 3 cut(s) 209, 704, 1036
DdeI CTNAG 2 cut(s) 1031, 1233
DpnI GATC 7 cut(s) 267, 297, 625, 663, 1243, 1290, 1299
DpnII GATC 7 cut(s) 265, 295, 623, 661, 1241, 1288, 1297
DrdI GACNNNNNNGTC 1 cut(s) 980
DseDI GACNNNNNNGTC 1 cut(s) 980
Eco130I CCWWGG 2 cut(s) 1180, 1195
Eco24I GRGCYC 1 cut(s) 954
Eco47I GGWCC 1 cut(s) 1043
Eco57I CTGAAG 3 cut(s) 486, 692, 1278
EcoRII CCWGG 3 cut(s) 362, 397, 1065
EcoT14I CCWWGG 2 cut(s) 1180, 1195
EcoT38I GRGCYC 1 cut(s) 954
ErhI CCWWGG 2 cut(s) 1180, 1195
FaeI CATG 6 cut(s) 49, 302, 668, 913, 958, 1241
FalI AAGNNNNNCTT 2 cut(s) 1014, 1046
FatI CATG 6 cut(s) 45, 298, 664, 909, 954, 1237
FbaI TGATCA 1 cut(s) 661
Fnu4HI GCNGC 5 cut(s) 6, 9, 36, 204, 764
FokI GGATG 6 cut(s) 25, 251, 345, 671, 902, 1139
FriOI GRGCYC 1 cut(s) 954
Fsp4HI GCNGC 5 cut(s) 6, 9, 36, 204, 764
FspBI CTAG 4 cut(s) 947, 1151, 1181, 1292
GlaI GCGC 2 cut(s) 172, 229
GluI GCNGC 5 cut(s) 6, 9, 36, 204, 764
GsuI CTGGAG 1 cut(s) 420
HaeII RGCGCY 1 cut(s) 231
HaeIII GGCC 1 cut(s) 203
HapII CCGG 1 cut(s) 337
HhaI GCGC 2 cut(s) 173, 230
Hin1II CATG 6 cut(s) 49, 302, 668, 913, 958, 1241
Hin6I GCGC 2 cut(s) 171, 228
HinP1I GCGC 2 cut(s) 171, 228
HincII GTYRAC 2 cut(s) 880, 1009
HindII GTYRAC 2 cut(s) 880, 1009
HinfI GANTC 3 cut(s) 327, 847, 1144
HpaII CCGG 1 cut(s) 337
Hpy166II GTNNAC 4 cut(s) 281, 874, 880, 1009
Hpy188I TCNGA 1 cut(s) 671
Hpy188III TCNNGA 6 cut(s) 260, 299, 427, 955, 1151, 1292
Hpy8I GTNNAC 4 cut(s) 281, 874, 880, 1009
HpyAV CCTTC 2 cut(s) 601, 1069
HpyCH4III ACNGT 5 cut(s) 421, 560, 605, 682, 703
HpyCH4IV ACGT 4 cut(s) 163, 629, 876, 1050
HpyCH4V TGCA 8 cut(s) 128, 179, 377, 443, 566, 779, 995, 1283
HpyF10VI GCNNNNNNNGC 3 cut(s) 104, 113, 596
HpyF3I CTNAG 2 cut(s) 1031, 1233
HpySE526I ACGT 4 cut(s) 163, 629, 876, 1050
Hsp92II CATG 6 cut(s) 49, 302, 668, 913, 958, 1241
HspAI GCGC 2 cut(s) 171, 228
Ksp22I TGATCA 1 cut(s) 661
KspI CCGCGG 1 cut(s) 207
Kzo9I GATC 7 cut(s) 265, 295, 623, 661, 1241, 1288, 1297
LmnI GCTCC 1 cut(s) 354
Lsp1109I GCAGC 2 cut(s) 47, 775
LweI GCATC 6 cut(s) 47, 166, 229, 334, 392, 924
MaeI CTAG 4 cut(s) 947, 1151, 1181, 1292
MaeII ACGT 4 cut(s) 163, 629, 876, 1050
MaeIII GTNAC 2 cut(s) 535, 924
MalI GATC 7 cut(s) 267, 297, 625, 663, 1243, 1290, 1299
MboI GATC 7 cut(s) 265, 295, 623, 661, 1241, 1288, 1297
MflI RGATCY 3 cut(s) 623, 1288, 1297
MhlI GDGCHC 1 cut(s) 954
MmeI TCCRAC 2 cut(s) 649, 1104
MnlI CCTC 6 cut(s) 397, 515, 607, 953, 1056, 1288
MseI TTAA 1 cut(s) 786
MslI CAYNNNNRTG 1 cut(s) 561
MspA1I CMGCKG 2 cut(s) 206, 1091
MspI CCGG 1 cut(s) 337
MspR9I CCNGG 3 cut(s) 364, 399, 1067
MvaI CCWGG 3 cut(s) 364, 399, 1067
MvnI CGCG 1 cut(s) 206
MwoI GCNNNNNNNGC 3 cut(s) 104, 113, 596
NdeII GATC 7 cut(s) 265, 295, 623, 661, 1241, 1288, 1297
NlaIII CATG 6 cut(s) 49, 302, 668, 913, 958, 1241
NlaIV GGNNCC 3 cut(s) 356, 625, 1299
NmuCI GTSAC 1 cut(s) 535
PagI TCATGA 2 cut(s) 298, 954
PcsI WCGNNNNNNNCGW 1 cut(s) 981
PfeI GAWTC 3 cut(s) 327, 847, 1144
PfoI TCCNGGA 1 cut(s) 397
PkrI GCNGC 5 cut(s) 7, 10, 37, 205, 765
Ppu21I YACGTR 1 cut(s) 1051
PsiI TTATAA 1 cut(s) 17
Psp6I CCWGG 3 cut(s) 362, 397, 1065
PspGI CCWGG 3 cut(s) 362, 397, 1065
PspN4I GGNNCC 3 cut(s) 356, 625, 1299
PspPI GGNCC 2 cut(s) 201, 1043
PstNI CAGNNNCTG 1 cut(s) 686
PsuI RGATCY 3 cut(s) 623, 1288, 1297
RsaI GTAC 3 cut(s) 210, 705, 1037
RsaNI GTAC 3 cut(s) 209, 704, 1036
RseI CAYNNNNRTG 1 cut(s) 561
SacII CCGCGG 1 cut(s) 207
SaqAI TTAA 1 cut(s) 786
SatI GCNGC 5 cut(s) 6, 9, 36, 204, 764
Sau3AI GATC 7 cut(s) 265, 295, 623, 661, 1241, 1288, 1297
Sau96I GGNCC 2 cut(s) 201, 1043
ScrFI CCNGG 3 cut(s) 364, 399, 1067
SduI GDGCHC 1 cut(s) 954
SfaNI GCATC 6 cut(s) 47, 166, 229, 334, 392, 924
SfcI CTRYAG 1 cut(s) 601
Sfr303I CCGCGG 1 cut(s) 207
SgrBI CCGCGG 1 cut(s) 207
SinI GGWCC 1 cut(s) 1043
SmiMI CAYNNNNRTG 1 cut(s) 561
SsiI CCGC 6 cut(s) 8, 105, 204, 206, 798, 1089
SspMI CTAG 4 cut(s) 947, 1151, 1181, 1292
StyD4I CCNGG 3 cut(s) 362, 397, 1065
StyI CCWWGG 2 cut(s) 1180, 1195
TaaI ACNGT 5 cut(s) 421, 560, 605, 682, 703
TaiI ACGT 4 cut(s) 166, 632, 879, 1053
TaqI TCGA 2 cut(s) 857, 984
TatI WGTACW 1 cut(s) 703
TauI GCSGC 2 cut(s) 11, 206
TfiI GAWTC 3 cut(s) 327, 847, 1144
Tru1I TTAA 1 cut(s) 786
Tru9I TTAA 1 cut(s) 786
TscAI CASTG 5 cut(s) 135, 384, 498, 546, 706
TseFI GTSAC 1 cut(s) 535
TseI GCWGC 3 cut(s) 5, 35, 763
Tsp45I GTSAC 1 cut(s) 535
TspDTI ATGAA 5 cut(s) 315, 382, 583, 715, 1287
TspGWI ACGGA 2 cut(s) 31, 990
TspRI CASTG 5 cut(s) 135, 384, 498, 546, 706
VpaK11BI GGWCC 1 cut(s) 1043
XapI RAATTY 4 cut(s) 19, 254, 674, 1248
XbaI TCTAGA 2 cut(s) 1150, 1291
XmaJI CCTAGG 1 cut(s) 1180
XspI CTAG 4 cut(s) 947, 1151, 1181, 1292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.