Rh3DG235200

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0000950
Physical Location & Seq
Forward (+)
22472173 .. 22483513
11341 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG235200.1

Sequence Viewer

Length: 1587 bp
ATGCCCAGAATACTTGCAATAGTGAGAGACTGGTTTCAAAATTACCGCTACTTTTCCAGGGCAACAATATGCATCCAGGGTTTGGTTTTCAGCCATAGCTTCGCAACCCATGCCATAGTATCTATATTGATAACGTACCTTACGACTATGTGGAAGTATCCACATGCTCAGATAGCTGTTGTAGTGATGAATGTGCAAGATGGGGTATTAGACATTATGGTCATTGTCCTCGCGCATATTCCTCGCACGTACATCGGTTTGTTTAGAGTAATTGCCACCACCAATGCTTCATATATTATTGGGCTTCTTCTGTTATGGCTTTCTGCTGGGTATATGAGTGACAGTACTGCATCTAGGGTATTCTATGGAGCTATGGTACTAGTAACATTAGGCGAAGCTGGGCGGTGGGCTGCTTTGCGAGAATTTCTGGATGAACAATATTCAAGAGAACAAAATCATGAGGAACGCACATATGATGAAAATAAGGATAAGAAAGAAGCTTTGTGGATGCTTCCTTGGTTTTTAGGTGCAGTGATTCCTCTTTTCCTTTTAAAAACAACTTGGCCACAAAGATTCATGATATCAACAATTGCAATGACGGTTTCTTATTTAGGATTCTTGTTGGGCTTTCCCTATTATTTCACATACAATATGACTAATACTGAACAAGGCCTAGATGCTCGCCCAGAAGAAGTCAATTATCTAGAACAAGCACCAAGAGTCCCTGAACCAACAAGGTCAGGAAATGTATGGAGACTGATAAATATATTAAGGAAAGGTGAACGTTTTCTCATAGAAGCATTAGCTATATGGTTAGCCTTCCTCCTTGCATACAGTGTTGTAAAGGCAGCAGGAAGCACGTTTTTTTTTCAACAAATGGATAACTTGGATAACCCTTTCGATAACTATGATCCTGCAGTCTATTTCAATGTGTTTGCCTCATTTTCAAAGTACTTGATCTCATTTCTCTGGAACTTAATTCCAAGGAAATGGAAACCATCGCGGCTGAGGATTTGGTGCGGAATGGCTTGTATTGTGCTTTGTTGTCTTGTTGCTTCGCAAGTTGAGAGCCACAGGTTAAACACTTCTTCAAATACGAGCATCTTTTGGTTGGTTCCTCAGTTCTTCTTACTGGGAGTCATGGAAGGCCTAGCCATGTATGGAATGATCGATTTTCTTGTTGATCGAGTGTCTGAAGGTGATAAAGTAATGGTCGCATATTATGGATCCCACACCACTGAGTTGATCATAGGTATTGGAAAAATACTTGTTGGTCTTAGTGTTTTGGCGCTCAGAGGAAGCTGGTTTGATGACAACAACTTAGATGCCAGTCATCTTCATAAATATTACAGAGCATTAACAACCTTAACTTCTGTTGTCTTCTTCTTCCACTGTTGTATCGCCTTTTACTATTACACTAGGGAAGAATTACAAGATGAGACGCCTTCATCGGTGACAATTGAGATGGTGAATCAGAATCAGCCACAGATAGACCCGTCGCCACATAACCCTGATAATGCATTTACTGAGACGCTTGATGACAGAGTTGGGTTATCTTATTTATTGGGACAGTTTGTGGGTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

528

Amino Acids

60.64

Weight (kDa)

5.91

Isoelectric Point (pI)

37.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 218
AccB7I CCANNNNNTGG 1 cut(s) 82
AccII CGCG 2 cut(s) 233, 1003
AciI CCGC 4 cut(s) 46, 403, 1003, 1020
AclI AACGTT 1 cut(s) 784
AclWI GGATC 3 cut(s) 905, 1221, 1234
AcoI YGGCCR 1 cut(s) 563
AcsI RAATTY 1 cut(s) 422
AcuI CTGAAG 1 cut(s) 1215
AcyI GRCGYC 1 cut(s) 1442
AfaI GTAC 5 cut(s) 137, 251, 346, 378, 953
AfiI CCNNNNNNNGG 1 cut(s) 82
AgsI TTSAA 6 cut(s) 38, 444, 872, 928, 948, 1092
AhlI ACTAGT 1 cut(s) 379
AjnI CCWGG 2 cut(s) 56, 75
AluBI AGCT 7 cut(s) 99, 176, 371, 398, 500, 806, 1302
AluI AGCT 7 cut(s) 99, 176, 371, 398, 500, 806, 1302
Alw26I GTCTC 4 cut(s) 21, 748, 1433, 1523
AlwI GGATC 3 cut(s) 905, 1221, 1234
AoxI GGCC 3 cut(s) 563, 670, 1147
ApeKI GCWGC 2 cut(s) 410, 848
ApoI RAATTY 1 cut(s) 422
Asp700I GAANNNNTTC 1 cut(s) 786
AspLEI GCGC 2 cut(s) 235, 1291
AsuHPI GGTGA 4 cut(s) 791, 1211, 1465, 1480
BaeI ACNNNNGTAYC 2 cut(s) 1381, 1414
BalI TGGCCA 1 cut(s) 565
BamHI GGATCC 1 cut(s) 1226
BbsI GAAGAC 1 cut(s) 1372
BbvCI CCTCAGC 1 cut(s) 1007
BbvI GCAGC 2 cut(s) 397, 860
BccI CCATC 3 cut(s) 194, 1006, 1459
BcgI CGANNNNNNTGC 4 cut(s) 224, 235, 258, 269
BciT130I CCWGG 2 cut(s) 58, 77
BciVI GTATCC 1 cut(s) 168
BclI TGATCA 1 cut(s) 1245
BcoDI GTCTC 4 cut(s) 21, 748, 1433, 1523
BcuI ACTAGT 1 cut(s) 379
BfaI CTAG 6 cut(s) 354, 380, 674, 704, 1151, 1419
BfmI CTRYAG 1 cut(s) 915
BfoI RGCGCY 1 cut(s) 1292
BfuI GTATCC 1 cut(s) 168
BisI GCNGC 3 cut(s) 411, 849, 1004
BlsI GCNGC 3 cut(s) 412, 850, 1005
BmcAI AGTACT 2 cut(s) 346, 953
Bme1390I CCNGG 2 cut(s) 58, 77
BmiI GGNNCC 2 cut(s) 1116, 1228
BmrFI CCNGG 2 cut(s) 58, 77
BmrI ACTGGG 1 cut(s) 1142
BmsI GCATC 6 cut(s) 81, 359, 498, 667, 1110, 1315
BmuI ACTGGG 1 cut(s) 1142
BpiI GAAGAC 1 cut(s) 1372
Bpu10I CCTNAGC 1 cut(s) 1007
Bsa29I ATCGAT 1 cut(s) 1170
BsaAI YACGTR 1 cut(s) 249
BsaHI GRCGYC 1 cut(s) 1442
BsaJI CCNNGG 4 cut(s) 57, 76, 515, 983
BsaXI ACNNNNNCTCC 2 cut(s) 360, 390
Bsc4I CCNNNNNNNGG 1 cut(s) 82
Bse1I ACTGG 3 cut(s) 35, 1137, 1329
Bse3DI GCAATG 1 cut(s) 600
BseBI CCWGG 2 cut(s) 58, 77
BseCI ATCGAT 1 cut(s) 1170
BseDI CCNNGG 4 cut(s) 57, 76, 515, 983
BseGI GGATG 3 cut(s) 72, 436, 513
BseLI CCNNNNNNNGG 1 cut(s) 82
BseMI GCAATG 1 cut(s) 600
BseMII CTCAG 6 cut(s) 182, 998, 1133, 1230, 1306, 1518
BseNI ACTGG 3 cut(s) 35, 1137, 1329
BseXI GCAGC 2 cut(s) 397, 860
BseYI CCCAGC 2 cut(s) 326, 398
BsgI GTGCAG 1 cut(s) 549
Bsh1236I CGCG 2 cut(s) 233, 1003
BshFI GGCC 3 cut(s) 565, 672, 1149
BshVI ATCGAT 1 cut(s) 1170
BslFI GGGAC 2 cut(s) 707, 1581
BslI CCNNNNNNNGG 1 cut(s) 82
BsmAI GTCTC 4 cut(s) 21, 748, 1433, 1523
BsmBI CGTCTC 2 cut(s) 1433, 1523
BsmFI GGGAC 2 cut(s) 707, 1581
BsnI GGCC 3 cut(s) 565, 672, 1149
Bsp143I GATC 6 cut(s) 910, 957, 1167, 1183, 1226, 1245
BspACI CCGC 4 cut(s) 46, 403, 1003, 1020
BspANI GGCC 3 cut(s) 565, 672, 1149
BspCNI CTCAG 6 cut(s) 181, 999, 1132, 1231, 1305, 1519
BspDI ATCGAT 1 cut(s) 1170
BspFNI CGCG 2 cut(s) 233, 1003
BspHI TCATGA 2 cut(s) 457, 576
BspLI GGNNCC 2 cut(s) 1116, 1228
BspMAI CTGCAG 1 cut(s) 919
BspPI GGATC 3 cut(s) 905, 1221, 1234
BsrDI GCAATG 1 cut(s) 600
BsrI ACTGG 3 cut(s) 35, 1137, 1329
BssECI CCNNGG 4 cut(s) 57, 76, 515, 983
BssMI GATC 6 cut(s) 910, 957, 1167, 1183, 1226, 1245
BssNI GRCGYC 1 cut(s) 1442
BssT1I CCWWGG 2 cut(s) 515, 983
Bst2UI CCWGG 2 cut(s) 58, 77
Bst4CI ACNGT 5 cut(s) 344, 601, 836, 1394, 1572
BstACI GRCGYC 1 cut(s) 1442
BstAPI GCANNNNNTGC 1 cut(s) 110
BstBAI YACGTR 1 cut(s) 249
BstC8I GCNNGC 1 cut(s) 682
BstDEI CTNAG 8 cut(s) 168, 1007, 1119, 1239, 1277, 1292, 1321, 1527
BstF5I GGATG 3 cut(s) 72, 436, 513
BstFNI CGCG 2 cut(s) 233, 1003
BstH2I RGCGCY 1 cut(s) 1292
BstHHI GCGC 2 cut(s) 235, 1291
BstKTI GATC 6 cut(s) 913, 960, 1170, 1186, 1229, 1248
BstMAI GTCTC 4 cut(s) 21, 748, 1433, 1523
BstMBI GATC 6 cut(s) 910, 957, 1167, 1183, 1226, 1245
BstMWI GCNNNNNNNGC 2 cut(s) 110, 173
BstNI CCWGG 2 cut(s) 58, 77
BstNSI RCATGY 1 cut(s) 167
BstSCI CCNGG 2 cut(s) 56, 75
BstSFI CTRYAG 1 cut(s) 915
BstUI CGCG 2 cut(s) 233, 1003
BstV1I GCAGC 2 cut(s) 397, 860
BstV2I GAAGAC 1 cut(s) 1372
BstX2I RGATCY 1 cut(s) 1226
BstXI CCANNNNNNTGG 1 cut(s) 990
BstYI RGATCY 1 cut(s) 1226
Bsu15I ATCGAT 1 cut(s) 1170
BsuI GTATCC 1 cut(s) 168
BsuRI GGCC 3 cut(s) 565, 672, 1149
BsuTUI ATCGAT 1 cut(s) 1170
BtgZI GCGATG 1 cut(s) 984
BtsCI GGATG 3 cut(s) 72, 436, 513
BtsI GCAGTG 1 cut(s) 537
BtsIMutI CAGTG 4 cut(s) 537, 841, 1236, 1390
Cac8I GCNNGC 1 cut(s) 682
CciI TCATGA 2 cut(s) 457, 576
CfoI GCGC 2 cut(s) 235, 1291
ClaI ATCGAT 1 cut(s) 1170
CseI GACGC 2 cut(s) 1450, 1540
Csp6I GTAC 5 cut(s) 136, 250, 345, 377, 952
CviAII CATG 6 cut(s) 110, 164, 458, 577, 1141, 1156
CviQI GTAC 5 cut(s) 136, 250, 345, 377, 952
DdeI CTNAG 8 cut(s) 168, 1007, 1119, 1239, 1277, 1292, 1321, 1527
DpnI GATC 6 cut(s) 912, 959, 1169, 1185, 1228, 1247
DpnII GATC 6 cut(s) 910, 957, 1167, 1183, 1226, 1245
DraI TTTAAA 1 cut(s) 552
DrdI GACNNNNNNGTC 1 cut(s) 218
DseDI GACNNNNNNGTC 1 cut(s) 218
EaeI YGGCCR 1 cut(s) 563
Eco130I CCWWGG 2 cut(s) 515, 983
Eco147I AGGCCT 2 cut(s) 672, 1149
Eco32I GATATC 1 cut(s) 582
Eco57I CTGAAG 1 cut(s) 1215
EcoRII CCWGG 2 cut(s) 56, 75
EcoRV GATATC 1 cut(s) 582
EcoT14I CCWWGG 2 cut(s) 515, 983
EcoT22I ATGCAT 2 cut(s) 74, 1522
ErhI CCWWGG 2 cut(s) 515, 983
Esp3I CGTCTC 2 cut(s) 1433, 1523
FaeI CATG 6 cut(s) 113, 167, 461, 580, 1144, 1159
FaqI GGGAC 2 cut(s) 707, 1581
FatI CATG 6 cut(s) 109, 163, 457, 576, 1140, 1155
FauNDI CATATG 1 cut(s) 472
FbaI TGATCA 1 cut(s) 1245
Fnu4HI GCNGC 3 cut(s) 411, 849, 1004
FokI GGATG 3 cut(s) 59, 443, 520
Fsp4HI GCNGC 3 cut(s) 411, 849, 1004
FspBI CTAG 6 cut(s) 354, 380, 674, 704, 1151, 1419
GlaI GCGC 2 cut(s) 234, 1290
GluI GCNGC 3 cut(s) 411, 849, 1004
GsaI CCCAGC 2 cut(s) 330, 402
HaeII RGCGCY 1 cut(s) 1292
HaeIII GGCC 3 cut(s) 565, 672, 1149
HgaI GACGC 2 cut(s) 1450, 1540
HhaI GCGC 2 cut(s) 235, 1291
Hin1I GRCGYC 1 cut(s) 1442
Hin1II CATG 6 cut(s) 113, 167, 461, 580, 1144, 1159
Hin6I GCGC 2 cut(s) 233, 1289
HinP1I GCGC 2 cut(s) 233, 1289
HindIII AAGCTT 1 cut(s) 498
HinfI GANTC 7 cut(s) 535, 573, 615, 720, 1137, 1471, 1477
HphI GGTGA 4 cut(s) 791, 1211, 1465, 1480
Hpy166II GTNNAC 1 cut(s) 782
Hpy188I TCNGA 4 cut(s) 171, 1195, 1295, 1476
Hpy188III TCNNGA 7 cut(s) 428, 444, 458, 577, 704, 741, 970
Hpy8I GTNNAC 1 cut(s) 782
Hpy99I CGWCG 1 cut(s) 1501
HpyAV CCTTC 4 cut(s) 829, 1139, 1190, 1455
HpyCH4III ACNGT 5 cut(s) 344, 601, 836, 1394, 1572
HpyCH4IV ACGT 4 cut(s) 134, 248, 784, 860
HpyCH4V TGCA 9 cut(s) 17, 72, 196, 350, 530, 593, 830, 917, 1520
HpyF10VI GCNNNNNNNGC 2 cut(s) 110, 173
HpyF3I CTNAG 8 cut(s) 168, 1007, 1119, 1239, 1277, 1292, 1321, 1527
HpySE526I ACGT 4 cut(s) 134, 248, 784, 860
Hsp92I GRCGYC 1 cut(s) 1442
Hsp92II CATG 6 cut(s) 113, 167, 461, 580, 1144, 1159
HspAI GCGC 2 cut(s) 233, 1289
Ksp22I TGATCA 1 cut(s) 1245
Kzo9I GATC 6 cut(s) 910, 957, 1167, 1183, 1226, 1245
LmnI GCTCC 1 cut(s) 368
Lsp1109I GCAGC 2 cut(s) 397, 860
LweI GCATC 6 cut(s) 81, 359, 498, 667, 1110, 1315
MaeI CTAG 6 cut(s) 354, 380, 674, 704, 1151, 1419
MaeII ACGT 4 cut(s) 134, 248, 784, 860
MaeIII GTNAC 3 cut(s) 338, 382, 1453
MalI GATC 6 cut(s) 912, 959, 1169, 1185, 1228, 1247
MboI GATC 6 cut(s) 910, 957, 1167, 1183, 1226, 1245
MboII GAAGA 9 cut(s) 299, 701, 1080, 1117, 1328, 1372, 1375, 1378, 1436
MfeI CAATTG 2 cut(s) 588, 1458
MflI RGATCY 1 cut(s) 1226
MlsI TGGCCA 1 cut(s) 565
MluCI AATT 8 cut(s) 40, 270, 422, 588, 697, 978, 1427, 1458
MluNI TGGCCA 1 cut(s) 565
MlyI GAGTC 2 cut(s) 729, 1146
MnlI CCTC 9 cut(s) 239, 252, 454, 549, 833, 949, 1002, 1128, 1289
Mox20I TGGCCA 1 cut(s) 565
Mph1103I ATGCAT 2 cut(s) 74, 1522
MroXI GAANNNNTTC 1 cut(s) 786
MscI TGGCCA 1 cut(s) 565
MseI TTAA 6 cut(s) 551, 770, 977, 1079, 1358, 1367
Msp20I TGGCCA 1 cut(s) 565
MspR9I CCNGG 2 cut(s) 58, 77
MunI CAATTG 2 cut(s) 588, 1458
MvaI CCWGG 2 cut(s) 58, 77
MvnI CGCG 2 cut(s) 233, 1003
MwoI GCNNNNNNNGC 2 cut(s) 110, 173
NdeI CATATG 1 cut(s) 472
NdeII GATC 6 cut(s) 910, 957, 1167, 1183, 1226, 1245
NlaIII CATG 6 cut(s) 113, 167, 461, 580, 1144, 1159
NlaIV GGNNCC 2 cut(s) 1116, 1228
NmuCI GTSAC 2 cut(s) 338, 1453
NsiI ATGCAT 2 cut(s) 74, 1522
NspI RCATGY 1 cut(s) 167
PagI TCATGA 2 cut(s) 457, 576
PceI AGGCCT 2 cut(s) 672, 1149
PcsI WCGNNNNNNNCGW 1 cut(s) 140
PdmI GAANNNNTTC 1 cut(s) 786
PfeI GAWTC 5 cut(s) 535, 573, 615, 1471, 1477
PflMI CCANNNNNTGG 1 cut(s) 82
PkrI GCNGC 3 cut(s) 412, 850, 1005
PleI GAGTC 2 cut(s) 728, 1145
PpsI GAGTC 2 cut(s) 728, 1145
Ppu21I YACGTR 1 cut(s) 249
Psp1406I AACGTT 1 cut(s) 784
Psp6I CCWGG 2 cut(s) 56, 75
PspFI CCCAGC 2 cut(s) 326, 398
PspGI CCWGG 2 cut(s) 56, 75
PspN4I GGNNCC 2 cut(s) 1116, 1228
PstI CTGCAG 1 cut(s) 919
PsuI RGATCY 1 cut(s) 1226
RsaI GTAC 5 cut(s) 137, 251, 346, 378, 953
RsaNI GTAC 5 cut(s) 136, 250, 345, 377, 952
SaqAI TTAA 6 cut(s) 551, 770, 977, 1079, 1358, 1367
SatI GCNGC 3 cut(s) 411, 849, 1004
Sau3AI GATC 6 cut(s) 910, 957, 1167, 1183, 1226, 1245
ScaI AGTACT 2 cut(s) 346, 953
SchI GAGTC 2 cut(s) 729, 1146
ScrFI CCNGG 2 cut(s) 58, 77
SfaNI GCATC 6 cut(s) 81, 359, 498, 667, 1110, 1315
SfcI CTRYAG 1 cut(s) 915
SpeI ACTAGT 1 cut(s) 379
Sse9I AATT 8 cut(s) 40, 270, 422, 588, 697, 978, 1427, 1458
SseBI AGGCCT 2 cut(s) 672, 1149
SsiI CCGC 4 cut(s) 46, 403, 1003, 1020
SspI AATATT 2 cut(s) 440, 1346
SspMI CTAG 6 cut(s) 354, 380, 674, 704, 1151, 1419
StuI AGGCCT 2 cut(s) 672, 1149
StyD4I CCNGG 2 cut(s) 56, 75
StyI CCWWGG 2 cut(s) 515, 983
TaaI ACNGT 5 cut(s) 344, 601, 836, 1394, 1572
TaiI ACGT 4 cut(s) 137, 251, 787, 863
TaqI TCGA 3 cut(s) 900, 1170, 1186
TasI AATT 8 cut(s) 40, 270, 422, 588, 697, 978, 1427, 1458
TatI WGTACW 2 cut(s) 344, 951
TauI GCSGC 1 cut(s) 1006
TfiI GAWTC 5 cut(s) 535, 573, 615, 1471, 1477
Tru1I TTAA 6 cut(s) 551, 770, 977, 1079, 1358, 1367
Tru9I TTAA 6 cut(s) 551, 770, 977, 1079, 1358, 1367
TscAI CASTG 4 cut(s) 537, 841, 1243, 1397
TseFI GTSAC 2 cut(s) 338, 1453
TseI GCWGC 2 cut(s) 410, 848
Tsp45I GTSAC 2 cut(s) 338, 1453
TspDTI ATGAA 7 cut(s) 203, 279, 447, 492, 565, 1328, 1437
TspRI CASTG 4 cut(s) 537, 841, 1243, 1397
Van91I CCANNNNNTGG 1 cut(s) 82
XapI RAATTY 1 cut(s) 422
XbaI TCTAGA 1 cut(s) 703
XceI RCATGY 1 cut(s) 167
XmnI GAANNNNTTC 1 cut(s) 786
XspI CTAG 6 cut(s) 354, 380, 674, 704, 1151, 1419
ZrmI AGTACT 2 cut(s) 346, 953
Zsp2I ATGCAT 2 cut(s) 74, 1522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.