Rw3G019030

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0000950
Physical Location & Seq
Forward (+)
21449747 .. 21459345
9599 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G019030.1

Sequence Viewer

Length: 1671 bp
ATGATATATTGCAGGTTCGTGCTTCGAAAAGCAGGCTCGATTGGGCGCTTCACTTTCCCTACATCCATTCGAGACTTTCACACAATGCCCAGAATACTTGCAATAGTGAGACACTGGTTTCAAAATTACCGCTACTTTTCCAGGGCTACAATATGCATCCAGGGTTTGGTTTTCAGCCATAGCTTCGCAACCCATGCCATAGTATCTATATTGATAACGTACCTTACGACTATGTGGAAGTATCCACATGCTCAGATAGCTGTTGTAGTGATGAATGTGCAAGATGGGGTATTAGACATTATGGTCATTATCCTCGCGCATATTCCTCGCACGTACATCGGTTTGTTTAGAGTAATTGCCACCACCAATGCTTCATATATTATTGGGCTTCTTCTGTTATGGCTTTCTGCTGGGTATATGAGTGACGGTACTGCATCTAGGGTATTCTATGGAGCTATGGTACTAGTAACATTAGGCGAAGCTGGGCGGTGGGCTGCTTTGCGAGAATTTCTGGATGAACAATATTCAAGAGAACAAAATCATGAGGAACGCACATATGATGAAAATAAGGATAAGAAAGAAGCTTTGTGGATGCTTCCTTGGTTTTTAGGTGCAGTGATTCCTCTTTTCCTTTTAAAAACAACTTGGCCACAAAGATTCATGATATCAACAATTGCAATGACGGTTTCTTATTTAGGATTCTTGTTGGGCTTTCCCTATTATTTCACATACAATATGACTAATACTGAACAAGGCCAAGATGCTCGCCCAGAAGAAGTCAATTATCAAGAACAAGCACCAAGAGTCCCTGAACCAACAAGGTCAGGAAATGTATGGAGACTGATAAATATATTAAGGAAAGGTGAACGTTTTCTCATAGAAGCATTAGCTATATGGTTAGCCTTCCTCCTTGCATACAGTGTTGTAAAGGCAGCAGGAAGCACGTTTTTTTTTCAACAAATGGATAACTTGGATAACCCTTTCGATAACTATGATCCTGCAGTCTATTTCAATGTGTTTGCCTCATTTTCAAAGTACTTGATCTCATTTCTCTGGAACTTAATTCCAAGGAAATGGAAACCATCGCGGCTGAGGATTTGGTGCGGAATGGCTTGTATTGTGCTTTGTTGTCTTGTTGCTTCGCAAGTTGAGAGCCACAGGTTAAACACTTCTTCAAATACGAGCATCTTTTGGTTGGTTCCTCAGTTCTTCTTACTGGGAGTCATGGAAGGCCTAGCCATGTATGGAATGATCGATTTTCTTGTTGATCGAGTGTCTGAAGGTGATAAAGTAATGGTCGCATATTATGGATCCCACACCACTGAGTTGATCATAGGTATTGGAAAAATACTTGTTGGTCTTAGTGTTTTGGCGCTCAGAGGAAGCTGGTTTGATGACAACAACTTAGATGCCAGTCATCTTCATAAATATTACAGAGCATTAACAACCTTAACTTCTGTTGTCTTCTTCTTCCACTGTTGTATCGCCTTTTACTATTACACTAGGGAAGAATTACAAGATGAGACGCCTTCATCGGTGACAATTGAGATGGTGAATCAGAATCAGCCACAGATAGACCCGTCGCCACATAACCCTGATAATGCATTTACTGAGACGCTTGATGACAGAGTTGGGTTATCTTATTTATTGGGACAGTTTGTGGGTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

556

Amino Acids

63.98

Weight (kDa)

6.68

Isoelectric Point (pI)

37.92

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 302
Acc36I ACCTGC 1 cut(s) 3
AccB7I CCANNNNNTGG 1 cut(s) 166
AccII CGCG 2 cut(s) 317, 1087
AciI CCGC 4 cut(s) 130, 487, 1087, 1104
AclI AACGTT 1 cut(s) 868
AclWI GGATC 3 cut(s) 989, 1305, 1318
AcoI YGGCCR 1 cut(s) 647
AcsI RAATTY 1 cut(s) 506
AcuI CTGAAG 1 cut(s) 1299
AcyI GRCGYC 1 cut(s) 1526
AfaI GTAC 5 cut(s) 221, 335, 430, 462, 1037
AfiI CCNNNNNNNGG 1 cut(s) 166
AgsI TTSAA 6 cut(s) 122, 528, 956, 1012, 1032, 1176
AhlI ACTAGT 1 cut(s) 463
AjnI CCWGG 2 cut(s) 140, 159
AluBI AGCT 7 cut(s) 183, 260, 455, 482, 584, 890, 1386
AluI AGCT 7 cut(s) 183, 260, 455, 482, 584, 890, 1386
Alw26I GTCTC 5 cut(s) 66, 103, 832, 1517, 1607
AlwI GGATC 3 cut(s) 989, 1305, 1318
AoxI GGCC 3 cut(s) 647, 754, 1231
ApeKI GCWGC 2 cut(s) 494, 932
ApoI RAATTY 1 cut(s) 506
Asp700I GAANNNNTTC 1 cut(s) 870
AspLEI GCGC 3 cut(s) 48, 319, 1375
AsuHPI GGTGA 4 cut(s) 875, 1295, 1549, 1564
AsuII TTCGAA 1 cut(s) 25
BaeI ACNNNNGTAYC 2 cut(s) 1465, 1498
BalI TGGCCA 1 cut(s) 649
BamHI GGATCC 1 cut(s) 1310
BbsI GAAGAC 1 cut(s) 1456
BbvCI CCTCAGC 1 cut(s) 1091
BbvI GCAGC 2 cut(s) 481, 944
BccI CCATC 3 cut(s) 278, 1090, 1543
BcgI CGANNNNNNTGC 4 cut(s) 308, 319, 342, 353
BciT130I CCWGG 2 cut(s) 142, 161
BciVI GTATCC 1 cut(s) 252
BclI TGATCA 1 cut(s) 1329
BcoDI GTCTC 5 cut(s) 66, 103, 832, 1517, 1607
BcuI ACTAGT 1 cut(s) 463
BfaI CTAG 4 cut(s) 438, 464, 1235, 1503
BfmI CTRYAG 1 cut(s) 999
BfoI RGCGCY 2 cut(s) 49, 1376
BfuAI ACCTGC 1 cut(s) 3
BfuI GTATCC 1 cut(s) 252
BisI GCNGC 3 cut(s) 495, 933, 1088
BlsI GCNGC 3 cut(s) 496, 934, 1089
BmcAI AGTACT 1 cut(s) 1037
Bme1390I CCNGG 2 cut(s) 142, 161
BmiI GGNNCC 2 cut(s) 1200, 1312
BmrFI CCNGG 2 cut(s) 142, 161
BmrI ACTGGG 1 cut(s) 1226
BmsI GCATC 6 cut(s) 165, 443, 582, 751, 1194, 1399
BmuI ACTGGG 1 cut(s) 1226
BpiI GAAGAC 1 cut(s) 1456
Bpu10I CCTNAGC 1 cut(s) 1091
Bpu14I TTCGAA 1 cut(s) 25
Bsa29I ATCGAT 1 cut(s) 1254
BsaAI YACGTR 1 cut(s) 333
BsaHI GRCGYC 1 cut(s) 1526
BsaJI CCNNGG 4 cut(s) 141, 160, 599, 1067
BsaXI ACNNNNNCTCC 2 cut(s) 444, 474
Bsc4I CCNNNNNNNGG 1 cut(s) 166
Bse1I ACTGG 3 cut(s) 119, 1221, 1413
Bse3DI GCAATG 1 cut(s) 684
BseBI CCWGG 2 cut(s) 142, 161
BseCI ATCGAT 1 cut(s) 1254
BseDI CCNNGG 4 cut(s) 141, 160, 599, 1067
BseGI GGATG 4 cut(s) 62, 156, 520, 597
BseLI CCNNNNNNNGG 1 cut(s) 166
BseMI GCAATG 1 cut(s) 684
BseMII CTCAG 6 cut(s) 266, 1082, 1217, 1314, 1390, 1602
BseNI ACTGG 3 cut(s) 119, 1221, 1413
BseXI GCAGC 2 cut(s) 481, 944
BseYI CCCAGC 2 cut(s) 410, 482
BsgI GTGCAG 1 cut(s) 633
Bsh1236I CGCG 2 cut(s) 317, 1087
BshFI GGCC 3 cut(s) 649, 756, 1233
BshVI ATCGAT 1 cut(s) 1254
BslFI GGGAC 2 cut(s) 791, 1665
BslI CCNNNNNNNGG 1 cut(s) 166
BsmAI GTCTC 5 cut(s) 66, 103, 832, 1517, 1607
BsmBI CGTCTC 2 cut(s) 1517, 1607
BsmFI GGGAC 2 cut(s) 791, 1665
BsnI GGCC 3 cut(s) 649, 756, 1233
Bsp119I TTCGAA 1 cut(s) 25
Bsp143I GATC 6 cut(s) 994, 1041, 1251, 1267, 1310, 1329
BspACI CCGC 4 cut(s) 130, 487, 1087, 1104
BspANI GGCC 3 cut(s) 649, 756, 1233
BspCNI CTCAG 6 cut(s) 265, 1083, 1216, 1315, 1389, 1603
BspDI ATCGAT 1 cut(s) 1254
BspFNI CGCG 2 cut(s) 317, 1087
BspHI TCATGA 2 cut(s) 541, 660
BspLI GGNNCC 2 cut(s) 1200, 1312
BspMAI CTGCAG 1 cut(s) 1003
BspMI ACCTGC 1 cut(s) 3
BspPI GGATC 3 cut(s) 989, 1305, 1318
BspT104I TTCGAA 1 cut(s) 25
BsrDI GCAATG 1 cut(s) 684
BsrI ACTGG 3 cut(s) 119, 1221, 1413
BssECI CCNNGG 4 cut(s) 141, 160, 599, 1067
BssMI GATC 6 cut(s) 994, 1041, 1251, 1267, 1310, 1329
BssNI GRCGYC 1 cut(s) 1526
BssT1I CCWWGG 2 cut(s) 599, 1067
Bst2UI CCWGG 2 cut(s) 142, 161
Bst4CI ACNGT 5 cut(s) 428, 685, 920, 1478, 1656
BstACI GRCGYC 1 cut(s) 1526
BstAPI GCANNNNNTGC 1 cut(s) 194
BstBAI YACGTR 1 cut(s) 333
BstBI TTCGAA 1 cut(s) 25
BstC8I GCNNGC 2 cut(s) 34, 766
BstDEI CTNAG 8 cut(s) 252, 1091, 1203, 1323, 1361, 1376, 1405, 1611
BstF5I GGATG 4 cut(s) 62, 156, 520, 597
BstFNI CGCG 2 cut(s) 317, 1087
BstH2I RGCGCY 2 cut(s) 49, 1376
BstHHI GCGC 3 cut(s) 48, 319, 1375
BstKTI GATC 6 cut(s) 997, 1044, 1254, 1270, 1313, 1332
BstMAI GTCTC 5 cut(s) 66, 103, 832, 1517, 1607
BstMBI GATC 6 cut(s) 994, 1041, 1251, 1267, 1310, 1329
BstMWI GCNNNNNNNGC 2 cut(s) 194, 257
BstNI CCWGG 2 cut(s) 142, 161
BstNSI RCATGY 1 cut(s) 251
BstSCI CCNGG 2 cut(s) 140, 159
BstSFI CTRYAG 1 cut(s) 999
BstUI CGCG 2 cut(s) 317, 1087
BstV1I GCAGC 2 cut(s) 481, 944
BstV2I GAAGAC 1 cut(s) 1456
BstX2I RGATCY 1 cut(s) 1310
BstXI CCANNNNNNTGG 1 cut(s) 1074
BstYI RGATCY 1 cut(s) 1310
Bsu15I ATCGAT 1 cut(s) 1254
BsuI GTATCC 1 cut(s) 252
BsuRI GGCC 3 cut(s) 649, 756, 1233
BsuTUI ATCGAT 1 cut(s) 1254
BtgZI GCGATG 1 cut(s) 1068
BtsCI GGATG 4 cut(s) 62, 156, 520, 597
BtsI GCAGTG 1 cut(s) 621
BtsIMutI CAGTG 5 cut(s) 112, 621, 925, 1320, 1474
BveI ACCTGC 1 cut(s) 3
Cac8I GCNNGC 2 cut(s) 34, 766
CciI TCATGA 2 cut(s) 541, 660
CfoI GCGC 3 cut(s) 48, 319, 1375
ClaI ATCGAT 1 cut(s) 1254
CseI GACGC 2 cut(s) 1534, 1624
Csp6I GTAC 5 cut(s) 220, 334, 429, 461, 1036
CviAII CATG 6 cut(s) 194, 248, 542, 661, 1225, 1240
CviQI GTAC 5 cut(s) 220, 334, 429, 461, 1036
DdeI CTNAG 8 cut(s) 252, 1091, 1203, 1323, 1361, 1376, 1405, 1611
DpnI GATC 6 cut(s) 996, 1043, 1253, 1269, 1312, 1331
DpnII GATC 6 cut(s) 994, 1041, 1251, 1267, 1310, 1329
DraI TTTAAA 1 cut(s) 636
DrdI GACNNNNNNGTC 1 cut(s) 302
DseDI GACNNNNNNGTC 1 cut(s) 302
EaeI YGGCCR 1 cut(s) 647
Eco130I CCWWGG 2 cut(s) 599, 1067
Eco147I AGGCCT 1 cut(s) 1233
Eco32I GATATC 1 cut(s) 666
Eco57I CTGAAG 1 cut(s) 1299
EcoRII CCWGG 2 cut(s) 140, 159
EcoRV GATATC 1 cut(s) 666
EcoT14I CCWWGG 2 cut(s) 599, 1067
EcoT22I ATGCAT 2 cut(s) 158, 1606
ErhI CCWWGG 2 cut(s) 599, 1067
Esp3I CGTCTC 2 cut(s) 1517, 1607
FaeI CATG 6 cut(s) 197, 251, 545, 664, 1228, 1243
FaqI GGGAC 2 cut(s) 791, 1665
FatI CATG 6 cut(s) 193, 247, 541, 660, 1224, 1239
FauNDI CATATG 1 cut(s) 556
FbaI TGATCA 1 cut(s) 1329
Fnu4HI GCNGC 3 cut(s) 495, 933, 1088
FokI GGATG 4 cut(s) 49, 143, 527, 604
Fsp4HI GCNGC 3 cut(s) 495, 933, 1088
FspBI CTAG 4 cut(s) 438, 464, 1235, 1503
GlaI GCGC 3 cut(s) 47, 318, 1374
GluI GCNGC 3 cut(s) 495, 933, 1088
GsaI CCCAGC 2 cut(s) 414, 486
HaeII RGCGCY 2 cut(s) 49, 1376
HaeIII GGCC 3 cut(s) 649, 756, 1233
HgaI GACGC 2 cut(s) 1534, 1624
HhaI GCGC 3 cut(s) 48, 319, 1375
Hin1I GRCGYC 1 cut(s) 1526
Hin1II CATG 6 cut(s) 197, 251, 545, 664, 1228, 1243
Hin6I GCGC 3 cut(s) 46, 317, 1373
HinP1I GCGC 3 cut(s) 46, 317, 1373
HindIII AAGCTT 1 cut(s) 582
HinfI GANTC 7 cut(s) 619, 657, 699, 804, 1221, 1555, 1561
HphI GGTGA 4 cut(s) 875, 1295, 1549, 1564
Hpy166II GTNNAC 1 cut(s) 866
Hpy188I TCNGA 4 cut(s) 255, 1279, 1379, 1560
Hpy188III TCNNGA 8 cut(s) 71, 512, 528, 542, 661, 788, 825, 1054
Hpy8I GTNNAC 1 cut(s) 866
Hpy99I CGWCG 1 cut(s) 1585
HpyAV CCTTC 4 cut(s) 913, 1223, 1274, 1539
HpyCH4III ACNGT 5 cut(s) 428, 685, 920, 1478, 1656
HpyCH4IV ACGT 4 cut(s) 218, 332, 868, 944
HpyF10VI GCNNNNNNNGC 2 cut(s) 194, 257
HpyF3I CTNAG 8 cut(s) 252, 1091, 1203, 1323, 1361, 1376, 1405, 1611
HpySE526I ACGT 4 cut(s) 218, 332, 868, 944
Hsp92I GRCGYC 1 cut(s) 1526
Hsp92II CATG 6 cut(s) 197, 251, 545, 664, 1228, 1243
HspAI GCGC 3 cut(s) 46, 317, 1373
Ksp22I TGATCA 1 cut(s) 1329
Kzo9I GATC 6 cut(s) 994, 1041, 1251, 1267, 1310, 1329
LmnI GCTCC 1 cut(s) 452
Lsp1109I GCAGC 2 cut(s) 481, 944
LweI GCATC 6 cut(s) 165, 443, 582, 751, 1194, 1399
MaeI CTAG 4 cut(s) 438, 464, 1235, 1503
MaeII ACGT 4 cut(s) 218, 332, 868, 944
MaeIII GTNAC 3 cut(s) 422, 466, 1537
MalI GATC 6 cut(s) 996, 1043, 1253, 1269, 1312, 1331
MboI GATC 6 cut(s) 994, 1041, 1251, 1267, 1310, 1329
MboII GAAGA 9 cut(s) 383, 785, 1164, 1201, 1412, 1456, 1459, 1462, 1520
MfeI CAATTG 2 cut(s) 672, 1542
MflI RGATCY 1 cut(s) 1310
MlsI TGGCCA 1 cut(s) 649
MluCI AATT 8 cut(s) 124, 354, 506, 672, 781, 1062, 1511, 1542
MluNI TGGCCA 1 cut(s) 649
MlyI GAGTC 2 cut(s) 813, 1230
MnlI CCTC 9 cut(s) 323, 336, 538, 633, 917, 1033, 1086, 1212, 1373
Mox20I TGGCCA 1 cut(s) 649
Mph1103I ATGCAT 2 cut(s) 158, 1606
MroXI GAANNNNTTC 1 cut(s) 870
MscI TGGCCA 1 cut(s) 649
MseI TTAA 6 cut(s) 635, 854, 1061, 1163, 1442, 1451
Msp20I TGGCCA 1 cut(s) 649
MspR9I CCNGG 2 cut(s) 142, 161
MunI CAATTG 2 cut(s) 672, 1542
MvaI CCWGG 2 cut(s) 142, 161
MvnI CGCG 2 cut(s) 317, 1087
MwoI GCNNNNNNNGC 2 cut(s) 194, 257
NdeI CATATG 1 cut(s) 556
NdeII GATC 6 cut(s) 994, 1041, 1251, 1267, 1310, 1329
NlaIII CATG 6 cut(s) 197, 251, 545, 664, 1228, 1243
NlaIV GGNNCC 2 cut(s) 1200, 1312
NmuCI GTSAC 2 cut(s) 422, 1537
NsiI ATGCAT 2 cut(s) 158, 1606
NspI RCATGY 1 cut(s) 251
NspV TTCGAA 1 cut(s) 25
PagI TCATGA 2 cut(s) 541, 660
PceI AGGCCT 1 cut(s) 1233
PcsI WCGNNNNNNNCGW 1 cut(s) 224
PdmI GAANNNNTTC 1 cut(s) 870
PfeI GAWTC 5 cut(s) 619, 657, 699, 1555, 1561
PflMI CCANNNNNTGG 1 cut(s) 166
PkrI GCNGC 3 cut(s) 496, 934, 1089
PleI GAGTC 2 cut(s) 812, 1229
PpsI GAGTC 2 cut(s) 812, 1229
Ppu21I YACGTR 1 cut(s) 333
Psp1406I AACGTT 1 cut(s) 868
Psp6I CCWGG 2 cut(s) 140, 159
PspFI CCCAGC 2 cut(s) 410, 482
PspGI CCWGG 2 cut(s) 140, 159
PspN4I GGNNCC 2 cut(s) 1200, 1312
PstI CTGCAG 1 cut(s) 1003
PsuI RGATCY 1 cut(s) 1310
RsaI GTAC 5 cut(s) 221, 335, 430, 462, 1037
RsaNI GTAC 5 cut(s) 220, 334, 429, 461, 1036
SaqAI TTAA 6 cut(s) 635, 854, 1061, 1163, 1442, 1451
SatI GCNGC 3 cut(s) 495, 933, 1088
Sau3AI GATC 6 cut(s) 994, 1041, 1251, 1267, 1310, 1329
ScaI AGTACT 1 cut(s) 1037
SchI GAGTC 2 cut(s) 813, 1230
ScrFI CCNGG 2 cut(s) 142, 161
SfaNI GCATC 6 cut(s) 165, 443, 582, 751, 1194, 1399
SfcI CTRYAG 1 cut(s) 999
SfuI TTCGAA 1 cut(s) 25
SpeI ACTAGT 1 cut(s) 463
Sse9I AATT 8 cut(s) 124, 354, 506, 672, 781, 1062, 1511, 1542
SseBI AGGCCT 1 cut(s) 1233
SsiI CCGC 4 cut(s) 130, 487, 1087, 1104
SspI AATATT 2 cut(s) 524, 1430
SspMI CTAG 4 cut(s) 438, 464, 1235, 1503
StuI AGGCCT 1 cut(s) 1233
StyD4I CCNGG 2 cut(s) 140, 159
StyI CCWWGG 2 cut(s) 599, 1067
TaaI ACNGT 5 cut(s) 428, 685, 920, 1478, 1656
TaiI ACGT 4 cut(s) 221, 335, 871, 947
TaqI TCGA 6 cut(s) 25, 38, 70, 984, 1254, 1270
TasI AATT 8 cut(s) 124, 354, 506, 672, 781, 1062, 1511, 1542
TatI WGTACW 1 cut(s) 1035
TauI GCSGC 1 cut(s) 1090
TfiI GAWTC 5 cut(s) 619, 657, 699, 1555, 1561
Tru1I TTAA 6 cut(s) 635, 854, 1061, 1163, 1442, 1451
Tru9I TTAA 6 cut(s) 635, 854, 1061, 1163, 1442, 1451
TscAI CASTG 5 cut(s) 119, 621, 925, 1327, 1481
TseFI GTSAC 2 cut(s) 422, 1537
TseI GCWGC 2 cut(s) 494, 932
Tsp45I GTSAC 2 cut(s) 422, 1537
TspDTI ATGAA 7 cut(s) 287, 363, 531, 576, 649, 1412, 1521
TspRI CASTG 5 cut(s) 119, 621, 925, 1327, 1481
Van91I CCANNNNNTGG 1 cut(s) 166
XapI RAATTY 1 cut(s) 506
XceI RCATGY 1 cut(s) 251
XmnI GAANNNNTTC 1 cut(s) 870
XspI CTAG 4 cut(s) 438, 464, 1235, 1503
ZrmI AGTACT 1 cut(s) 1037
Zsp2I ATGCAT 2 cut(s) 158, 1606
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.