Rh4BG244300

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000950
Physical Location & Seq
Forward (+)
41107655 .. 41130316
22662 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG244300.1

Sequence Viewer

Length: 1512 bp
ATGCGGAAGCTCTTCAGTTCAGTAAGAAACTGGATCGGAAACTACATATACTTTTCCGAGGCTACTTTATGCATCCGGGGTTTGGTCTTGAGCCATAGCTTTGTAAACCATGTGGTAATATCAGTATTGATAACTTACCTTACGGTTGAAATTTGGAAGCCATCAAATGACAATTATCAGCTGGCTGCTATAGCTATAAATGTGCTAAATGGGGTATCATCCATCATGGTGTGCGTCCTTGCTTACATCTCAAACAAGGTCGGTCCCTTTAAGGTGATTGCCACCACAAATGCTGCCTATATTCTTGGACTAGTTCTGTTGTGGAGTTCATCCAAATATGCCGGCTCCTTATCCAATGCATCAGATAAAATATTCTACGGAGCCGGCTTACTGTTAGCACTAGGCGAGGCTGGACGGCCGGCTGCTTTGGGTAAATTTCTCGAGGACCAATATTTAAGTGAAATGTCTTGCAAGAAAAGATTACGCGAAGCAAGATTAAGAGAAGAAAATCATATTACGACCACAAACGAAGAGGATAGGCAAAAACGTGAGGAAACCCGTCAAGCCGCTTTGTGGAGCCATCCCTGGTTCATAGGTGCGGCTGCACCTCTTGCCCTTGTAAATAGATCTTGGTCAACTCAACTACTGCTCTCAATGATCGCGGTGGGAGTTTCTTACTTATTGTTCTGGTCTGGCGTCTTCGAGTATTCCAAAAATATTAGAACTGATCCAGCAGGAAGAGATACTGGACTTTGCGCAGCCATCAAAAGTCTCTCACCATCACCATCAGAAGAAACAGCAAGCCGGAAGTATCGGCTCAAACCCACTTCCAGAAAAGAAATGCTAGTCATGTGCTTAGCCTTCATAGTATACAGTGTGATGGAGGCAACAGGGAACACCTTCTTTTTCGATCAAATGAGTCTACTGAACTATCACATCGGGAACTTTGAAGTGCCCAGCATATATTTCGTTATACTCGAATCCTTGTCAAGCTCCGTAATCTCATTTCTCTGGAGGTTACTAGTTCCAAAACAGTTAAAAAGTGCTACGTTGGTCAGGATCGGCTGTGGTTTAGTTTGCTCTGTGCTATGTATGGTTGCTGCTTGGCAAGTTGAGATTCGTAGGAAAAATAAGATCAATACCGAATATACACATGACCCACAGATGGAAATTTCCATGAGCATTCTTTGGTTAATTCCACAATTCTCCCTGTTAGGGTTTATGAGAGGGTTAGCCGCAGATGGATTGATCGAGTTTTGTGCGGATAGAGTTGATGATGGTAATGATAAAAGAGAGGCCAGGTATTATGGATCTTACGCCAGTGAATTAGTCCTGGGTATTGGAACACTACTAACCGCCGTTAGTATTTTCGCGTTTAGACGAACTTGGTTGGCTCATGATATATACAAGAATCGTCTGGATAAGTACTACAGGGGACTAGTATTCATAGGTGTTGCTAACCTTTGCTATTACTTTTGTGTCCCCGTTTACTATTATACCAAAGGAGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

503

Amino Acids

56.63

Weight (kDa)

9.06

Isoelectric Point (pI)

38.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 278 - 421 1.1e-10 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 757
AccI GTMKAC 2 cut(s) 870, 922
AccII CGCG 3 cut(s) 486, 662, 1373
AciI CCGC 7 cut(s) 4, 567, 599, 662, 1236, 1262, 1356
AclWI GGATC 4 cut(s) 41, 722, 1067, 1318
AcoI YGGCCR 1 cut(s) 416
AcsI RAATTY 3 cut(s) 150, 434, 1170
AcyI GRCGYC 1 cut(s) 696
AfaI GTAC 1 cut(s) 1427
AfiI CCNNNNNNNGG 4 cut(s) 82, 573, 1165, 1215
AgsI TTSAA 2 cut(s) 149, 950
AhlI ACTAGT 3 cut(s) 310, 1021, 1438
AjnI CCWGG 3 cut(s) 584, 1298, 1332
AluBI AGCT 5 cut(s) 10, 99, 181, 194, 993
AluI AGCT 5 cut(s) 10, 99, 181, 194, 993
Alw26I GTCTC 1 cut(s) 776
AlwI GGATC 4 cut(s) 41, 722, 1067, 1318
Ama87I CYCGRG 1 cut(s) 440
AoxI GGCC 2 cut(s) 416, 1296
ApeKI GCWGC 6 cut(s) 185, 293, 422, 602, 758, 1100
ApoI RAATTY 3 cut(s) 150, 434, 1170
Asp700I GAANNNNTTC 2 cut(s) 11, 899
AspLEI GCGC 1 cut(s) 758
AspS9I GGNCC 2 cut(s) 263, 445
AsuC2I CCSGG 1 cut(s) 77
AsuHPI GGTGA 3 cut(s) 286, 768, 774
AvaI CYCGRG 1 cut(s) 440
AvaII GGWCC 2 cut(s) 263, 445
BaeGI GKGCMC 1 cut(s) 957
BbsI GAAGAC 1 cut(s) 691
BbvI GCAGC 6 cut(s) 172, 280, 409, 589, 770, 1087
BceAI ACGGC 2 cut(s) 431, 1343
BcgI CGANNNNNNTGC 4 cut(s) 949, 983, 1241, 1275
BciT130I CCWGG 3 cut(s) 586, 1300, 1334
BcnI CCSGG 1 cut(s) 77
BcoDI GTCTC 1 cut(s) 776
BcuI ACTAGT 3 cut(s) 310, 1021, 1438
BfaI CTAG 5 cut(s) 311, 401, 845, 1022, 1439
BfmI CTRYAG 2 cut(s) 189, 1429
BglII AGATCT 1 cut(s) 626
BisI GCNGC 9 cut(s) 186, 294, 423, 567, 600, 603, 759, 1101, 1236
BlpI GCTNAGC 1 cut(s) 856
BlsI GCNGC 9 cut(s) 187, 295, 424, 568, 601, 604, 760, 1102, 1237
BmcAI AGTACT 1 cut(s) 1427
Bme1390I CCNGG 4 cut(s) 77, 586, 1300, 1334
Bme18I GGWCC 2 cut(s) 263, 445
BmeT110I CYCGRG 1 cut(s) 440
BmgT120I GGNCC 2 cut(s) 263, 445
BmiI GGNNCC 4 cut(s) 265, 346, 382, 578
BmrFI CCNGG 4 cut(s) 77, 586, 1300, 1334
BmsI GCATC 2 cut(s) 81, 368
BpiI GAAGAC 1 cut(s) 691
BpmI CTGGAG 1 cut(s) 1033
Bpu1102I GCTNAGC 1 cut(s) 856
BpuEI CTTGAG 1 cut(s) 109
BpuMI CCSGG 1 cut(s) 77
BsaHI GRCGYC 1 cut(s) 696
BsaJI CCNNGG 4 cut(s) 57, 76, 584, 1333
Bsc4I CCNNNNNNNGG 4 cut(s) 82, 573, 1165, 1215
Bse118I RCCGGY 3 cut(s) 341, 383, 418
Bse1I ACTGG 3 cut(s) 35, 751, 1320
BseBI CCWGG 3 cut(s) 586, 1300, 1334
BseDI CCNNGG 4 cut(s) 57, 76, 584, 1333
BseGI GGATG 4 cut(s) 72, 218, 329, 580
BseLI CCNNNNNNNGG 4 cut(s) 82, 573, 1165, 1215
BseNI ACTGG 3 cut(s) 35, 751, 1320
BseSI GKGCMC 1 cut(s) 957
BseX3I CGGCCG 1 cut(s) 416
BseXI GCAGC 6 cut(s) 172, 280, 409, 589, 770, 1087
BseYI CCCAGC 1 cut(s) 956
BsgI GTGCAG 1 cut(s) 588
Bsh1236I CGCG 3 cut(s) 486, 662, 1373
Bsh1285I CGRYCG 1 cut(s) 419
BshFI GGCC 2 cut(s) 418, 1298
BsiEI CGRYCG 1 cut(s) 419
BsiHKCI CYCGRG 1 cut(s) 440
BsiSI CCGG 5 cut(s) 76, 342, 384, 419, 805
BslFI GGGAC 3 cut(s) 249, 1449, 1466
BslI CCNNNNNNNGG 4 cut(s) 82, 573, 1165, 1215
BsmAI GTCTC 1 cut(s) 776
BsmFI GGGAC 3 cut(s) 249, 1449, 1466
BsmI GAATGC 1 cut(s) 1182
BsnI GGCC 2 cut(s) 418, 1298
BsoBI CYCGRG 1 cut(s) 440
Bsp1286I GDGCHC 1 cut(s) 957
Bsp143I GATC 9 cut(s) 33, 626, 657, 727, 910, 1059, 1134, 1248, 1310
Bsp1720I GCTNAGC 1 cut(s) 856
BspACI CCGC 7 cut(s) 4, 567, 599, 662, 1236, 1262, 1356
BspANI GGCC 2 cut(s) 418, 1298
BspFNI CGCG 3 cut(s) 486, 662, 1373
BspHI TCATGA 1 cut(s) 1396
BspLI GGNNCC 4 cut(s) 265, 346, 382, 578
BspPI GGATC 4 cut(s) 41, 722, 1067, 1318
BspQI GCTCTTC 1 cut(s) 17
BsrFI RCCGGY 3 cut(s) 341, 383, 418
BsrI ACTGG 3 cut(s) 35, 751, 1320
BssAI RCCGGY 3 cut(s) 341, 383, 418
BssECI CCNNGG 4 cut(s) 57, 76, 584, 1333
BssMI GATC 9 cut(s) 33, 626, 657, 727, 910, 1059, 1134, 1248, 1310
BssNAI GTATAC 1 cut(s) 871
BssNI GRCGYC 1 cut(s) 696
Bst1107I GTATAC 1 cut(s) 871
Bst2UI CCWGG 3 cut(s) 586, 1300, 1334
Bst4CI ACNGT 4 cut(s) 145, 393, 875, 1035
Bst6I CTCTTC 3 cut(s) 17, 525, 733
BstACI GRCGYC 1 cut(s) 696
BstAPI GCANNNNNTGC 1 cut(s) 611
BstC8I GCNNGC 5 cut(s) 183, 343, 385, 420, 802
BstDEI CTNAG 1 cut(s) 856
BstF5I GGATG 4 cut(s) 72, 218, 329, 580
BstFNI CGCG 3 cut(s) 486, 662, 1373
BstHHI GCGC 1 cut(s) 758
BstKTI GATC 9 cut(s) 36, 629, 660, 730, 913, 1062, 1137, 1251, 1313
BstMAI GTCTC 1 cut(s) 776
BstMBI GATC 9 cut(s) 33, 626, 657, 727, 910, 1059, 1134, 1248, 1310
BstMCI CGRYCG 1 cut(s) 419
BstMWI GCNNNNNNNGC 2 cut(s) 191, 611
BstNI CCWGG 3 cut(s) 586, 1300, 1334
BstSCI CCNGG 4 cut(s) 75, 584, 1298, 1332
BstSFI CTRYAG 2 cut(s) 189, 1429
BstSLI GKGCMC 1 cut(s) 957
BstUI CGCG 3 cut(s) 486, 662, 1373
BstV1I GCAGC 6 cut(s) 172, 280, 409, 589, 770, 1087
BstV2I GAAGAC 1 cut(s) 691
BstX2I RGATCY 2 cut(s) 626, 1310
BstYI RGATCY 2 cut(s) 626, 1310
BstZ17I GTATAC 1 cut(s) 871
BstZI CGGCCG 1 cut(s) 416
BsuRI GGCC 2 cut(s) 418, 1298
BtsCI GGATG 4 cut(s) 72, 218, 329, 580
BtsIMutI CAGTG 2 cut(s) 880, 1327
Cac8I GCNNGC 5 cut(s) 183, 343, 385, 420, 802
CciI TCATGA 1 cut(s) 1396
CfoI GCGC 1 cut(s) 758
Cfr10I RCCGGY 3 cut(s) 341, 383, 418
Cfr13I GGNCC 2 cut(s) 263, 445
CseI GACGC 2 cut(s) 223, 685
Csp6I GTAC 1 cut(s) 1426
CviAII CATG 6 cut(s) 110, 226, 850, 1154, 1177, 1397
CviQI GTAC 1 cut(s) 1426
DdeI CTNAG 1 cut(s) 856
DpnI GATC 9 cut(s) 35, 628, 659, 729, 912, 1061, 1136, 1250, 1312
DpnII GATC 9 cut(s) 33, 626, 657, 727, 910, 1059, 1134, 1248, 1310
EaeI YGGCCR 1 cut(s) 416
EagI CGGCCG 1 cut(s) 416
Eam1104I CTCTTC 3 cut(s) 17, 525, 733
EarI CTCTTC 3 cut(s) 17, 525, 733
EclXI CGGCCG 1 cut(s) 416
Eco47I GGWCC 2 cut(s) 263, 445
Eco52I CGGCCG 1 cut(s) 416
Eco88I CYCGRG 1 cut(s) 440
EcoRII CCWGG 3 cut(s) 584, 1298, 1332
EcoT22I ATGCAT 2 cut(s) 74, 361
FaeI CATG 6 cut(s) 113, 229, 853, 1157, 1180, 1400
FaqI GGGAC 3 cut(s) 249, 1449, 1466
FatI CATG 6 cut(s) 109, 225, 849, 1153, 1176, 1396
FblI GTMKAC 2 cut(s) 870, 922
Fnu4HI GCNGC 9 cut(s) 186, 294, 423, 567, 600, 603, 759, 1101, 1236
FokI GGATG 4 cut(s) 59, 205, 316, 567
Fsp4HI GCNGC 9 cut(s) 186, 294, 423, 567, 600, 603, 759, 1101, 1236
FspBI CTAG 5 cut(s) 311, 401, 845, 1022, 1439
FspI TGCGCA 1 cut(s) 757
GlaI GCGC 1 cut(s) 757
GluI GCNGC 9 cut(s) 186, 294, 423, 567, 600, 603, 759, 1101, 1236
GsaI CCCAGC 1 cut(s) 960
GsuI CTGGAG 1 cut(s) 1033
HaeIII GGCC 2 cut(s) 418, 1298
HapII CCGG 5 cut(s) 76, 342, 384, 419, 805
HgaI GACGC 2 cut(s) 223, 685
HhaI GCGC 1 cut(s) 758
Hin1I GRCGYC 1 cut(s) 696
Hin1II CATG 6 cut(s) 113, 229, 853, 1157, 1180, 1400
Hin6I GCGC 1 cut(s) 756
HinP1I GCGC 1 cut(s) 756
HincII GTYRAC 1 cut(s) 636
HindII GTYRAC 1 cut(s) 636
HinfI GANTC 4 cut(s) 919, 980, 1117, 1411
HpaII CCGG 5 cut(s) 76, 342, 384, 419, 805
HphI GGTGA 3 cut(s) 286, 768, 774
Hpy166II GTNNAC 5 cut(s) 106, 636, 871, 923, 1489
Hpy188I TCNGA 4 cut(s) 38, 58, 364, 790
Hpy188III TCNNGA 8 cut(s) 88, 440, 831, 940, 1012, 1057, 1397, 1418
Hpy8I GTNNAC 5 cut(s) 106, 636, 871, 923, 1489
HpyAV CCTTC 2 cut(s) 871, 910
HpyCH4III ACNGT 4 cut(s) 145, 393, 875, 1035
HpyCH4IV ACGT 2 cut(s) 547, 1049
HpyCH4V TGCA 4 cut(s) 72, 359, 471, 605
HpyF10VI GCNNNNNNNGC 2 cut(s) 191, 611
HpyF3I CTNAG 1 cut(s) 856
HpySE526I ACGT 2 cut(s) 547, 1049
Hsp92I GRCGYC 1 cut(s) 696
Hsp92II CATG 6 cut(s) 113, 229, 853, 1157, 1180, 1400
HspAI GCGC 1 cut(s) 756
KroI GCCGGC 3 cut(s) 341, 383, 418
KroNI GCCGGC 3 cut(s) 343, 385, 420
Kzo9I GATC 9 cut(s) 33, 626, 657, 727, 910, 1059, 1134, 1248, 1310
LguI GCTCTTC 1 cut(s) 17
LmnI GCTCC 4 cut(s) 350, 380, 576, 998
Lsp1109I GCAGC 6 cut(s) 172, 280, 409, 589, 770, 1087
LweI GCATC 2 cut(s) 81, 368
MaeI CTAG 5 cut(s) 311, 401, 845, 1022, 1439
MaeII ACGT 2 cut(s) 547, 1049
MaeIII GTNAC 1 cut(s) 1017
MalI GATC 9 cut(s) 35, 628, 659, 729, 912, 1061, 1136, 1250, 1312
MboI GATC 9 cut(s) 33, 626, 657, 727, 910, 1059, 1134, 1248, 1310
MboII GAAGA 6 cut(s) 4, 515, 542, 691, 750, 803
MflI RGATCY 2 cut(s) 626, 1310
MhlI GDGCHC 1 cut(s) 957
MluCI AATT 7 cut(s) 150, 172, 434, 1170, 1194, 1202, 1325
MlyI GAGTC 1 cut(s) 928
Mph1103I ATGCAT 2 cut(s) 74, 361
MroNI GCCGGC 3 cut(s) 341, 383, 418
MroXI GAANNNNTTC 2 cut(s) 11, 899
MseI TTAA 5 cut(s) 270, 455, 497, 1037, 1193
MslI CAYNNNNRTG 1 cut(s) 227
MspA1I CMGCKG 1 cut(s) 181
MspI CCGG 5 cut(s) 76, 342, 384, 419, 805
MspR9I CCNGG 4 cut(s) 77, 586, 1300, 1334
Mva1269I GAATGC 1 cut(s) 1182
MvaI CCWGG 3 cut(s) 586, 1300, 1334
MvnI CGCG 3 cut(s) 486, 662, 1373
MwoI GCNNNNNNNGC 2 cut(s) 191, 611
NaeI GCCGGC 3 cut(s) 343, 385, 420
NciI CCSGG 1 cut(s) 77
NdeII GATC 9 cut(s) 33, 626, 657, 727, 910, 1059, 1134, 1248, 1310
NgoMIV GCCGGC 3 cut(s) 341, 383, 418
NlaIII CATG 6 cut(s) 113, 229, 853, 1157, 1180, 1400
NlaIV GGNNCC 4 cut(s) 265, 346, 382, 578
NsbI TGCGCA 1 cut(s) 757
NsiI ATGCAT 2 cut(s) 74, 361
PaeR7I CTCGAG 1 cut(s) 440
PagI TCATGA 1 cut(s) 1396
PciSI GCTCTTC 1 cut(s) 17
PcsI WCGNNNNNNNCGW 1 cut(s) 975
PctI GAATGC 1 cut(s) 1182
PdiI GCCGGC 3 cut(s) 343, 385, 420
PdmI GAANNNNTTC 2 cut(s) 11, 899
PfeI GAWTC 3 cut(s) 980, 1117, 1411
PkrI GCNGC 9 cut(s) 187, 295, 424, 568, 601, 604, 760, 1102, 1237
PleI GAGTC 1 cut(s) 927
PpsI GAGTC 1 cut(s) 927
Psp6I CCWGG 3 cut(s) 584, 1298, 1332
PspFI CCCAGC 1 cut(s) 956
PspGI CCWGG 3 cut(s) 584, 1298, 1332
PspN4I GGNNCC 4 cut(s) 265, 346, 382, 578
PspPI GGNCC 2 cut(s) 263, 445
PsuI RGATCY 2 cut(s) 626, 1310
PvuII CAGCTG 1 cut(s) 181
RsaI GTAC 1 cut(s) 1427
RsaNI GTAC 1 cut(s) 1426
RseI CAYNNNNRTG 1 cut(s) 227
SapI GCTCTTC 1 cut(s) 17
SaqAI TTAA 5 cut(s) 270, 455, 497, 1037, 1193
SatI GCNGC 9 cut(s) 186, 294, 423, 567, 600, 603, 759, 1101, 1236
Sau3AI GATC 9 cut(s) 33, 626, 657, 727, 910, 1059, 1134, 1248, 1310
Sau96I GGNCC 2 cut(s) 263, 445
ScaI AGTACT 1 cut(s) 1427
SchI GAGTC 1 cut(s) 928
ScrFI CCNGG 4 cut(s) 77, 586, 1300, 1334
SduI GDGCHC 1 cut(s) 957
SfaNI GCATC 2 cut(s) 81, 368
SfcI CTRYAG 2 cut(s) 189, 1429
Sfr274I CTCGAG 1 cut(s) 440
SinI GGWCC 2 cut(s) 263, 445
SlaI CTCGAG 1 cut(s) 440
SmiMI CAYNNNNRTG 1 cut(s) 227
SmlI CTYRAG 2 cut(s) 88, 440
SmoI CTYRAG 2 cut(s) 88, 440
SpeI ACTAGT 3 cut(s) 310, 1021, 1438
Sse9I AATT 7 cut(s) 150, 172, 434, 1170, 1194, 1202, 1325
SsiI CCGC 7 cut(s) 4, 567, 599, 662, 1236, 1262, 1356
SspI AATATT 3 cut(s) 372, 452, 718
SspMI CTAG 5 cut(s) 311, 401, 845, 1022, 1439
StyD4I CCNGG 4 cut(s) 75, 584, 1298, 1332
TaaI ACNGT 4 cut(s) 145, 393, 875, 1035
TaiI ACGT 2 cut(s) 550, 1052
TaqI TCGA 5 cut(s) 441, 702, 909, 978, 1251
TaqII GACCGA 1 cut(s) 251
TasI AATT 7 cut(s) 150, 172, 434, 1170, 1194, 1202, 1325
TatI WGTACW 1 cut(s) 1425
TauI GCSGC 3 cut(s) 569, 602, 1238
TfiI GAWTC 3 cut(s) 980, 1117, 1411
Tru1I TTAA 5 cut(s) 270, 455, 497, 1037, 1193
Tru9I TTAA 5 cut(s) 270, 455, 497, 1037, 1193
TscAI CASTG 2 cut(s) 880, 1327
TseI GCWGC 6 cut(s) 185, 293, 422, 602, 758, 1100
TspDTI ATGAA 4 cut(s) 318, 580, 853, 1435
TspGWI ACGGA 2 cut(s) 393, 985
TspRI CASTG 2 cut(s) 880, 1327
VpaK11BI GGWCC 2 cut(s) 263, 445
XapI RAATTY 3 cut(s) 150, 434, 1170
XhoI CTCGAG 1 cut(s) 440
XmiI GTMKAC 2 cut(s) 870, 922
XmnI GAANNNNTTC 2 cut(s) 11, 899
XspI CTAG 5 cut(s) 311, 401, 845, 1022, 1439
ZrmI AGTACT 1 cut(s) 1427
Zsp2I ATGCAT 2 cut(s) 74, 361
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.