Rroxscaffold_5G00334470

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0000950
Physical Location & Seq
Forward (+)
1750100 .. 1759416
9317 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00334470.1

Sequence Viewer

Length: 1575 bp
ATGTGGAAGTCAGTAGCAAATTGGTTCGCAAGCTACCTCTACTTTTCCAAGGCTACTTTATGCATCCGGGGTTTGGTCTTCAGCCATAGCTTCGTAAACCATTTGGTAGTGTCTGCCTTGATGGCGTACGTGCAGAATATTATATTGGAGCCTTCAATGGCTGCTGTAGCTACAAATATTATAAATGGGGTATCATCCATCATGGTTTGTTTCATTGCTTATATCTCAAACGCGGTTGGTCCTTTTAAAGTGATTGCCTTCACAAATGCTGCCTATTTTCTTGGACTAATTATACTCTGGATATCAGTACATTGGGAATCCGAAACTATAATATTTTGGGGAGCTGCGTTACTTGTAGCATTAGGCGAGGCTGGACGGCCGGCTTCTCTGGGTAGATTTCTTGAGGATCAATATTTACGTGAAACTCACGACTTCAAATCACCCGAGAGAAAAAACAATCCTGAAGCCGAGGAAGATCATACTTTGACCAGAAAAGAAGAAGAAGAAGATGATAAGCGAAAACGTGCTTTGTGGAGCCATCCCTGGTTCTTAGGTGCAGCTGTAACTCTTGGCGTCATAAAGAGATCTGGTGAAACTCAATTCCTGCTTTCGACCATCGCGATGGGAGTTGCTTACTTATTGTTCTGGTATGGCATCTTCTTATATTCCAGAAATAATAGCCCAGCTGCAGAAGCAACACATAGAGATCAGGGCCTTTTCCAAGCCATCAAAAGACTCACCCCACCAAAAGAAGAAGCAAGGTGGAGATTTTGGCTGAAACCCAAATCCGGATCCAGAAAACATTATTTCAAAGAAATGTTAGTTATGTGCTTAGCTTTCATCGCATACAGTCTGGTCGAGGCAACGGGGAGCACCTTCTTTTTCTCCCAAATTACACTAGTACTACTGCATAAGGGGGAAATCCCAGCCGTCACTTATTTCGTTATCCTCCAATCCTTGTCAAGCTCGATAATCTCATTTTTCTGGAGTTTACTAGCTCCAAATCGGTCAAAACGTGCTACATTGTTGAGGATCGGCTGCGGATTGGTTTGTTCTGTGCTATGTATGGTTGTTGCTTGGCGAGTTGCGATTCATATAAATAAATCTGGAAATATTTACTCGAGGCTTGTTTGGTTGATTCCACAATTCTGCCTGTTAGGGTGCATGAGAGGGTTTGCCATGGATGGATTGCTCGAGTTTTTTGCGGATCGAGTCGATGGCAATGATAATGGTAAAAGAAAGGCTAGGTACTATGGAACTTATGCCAGTGAATTAGTCCTTGGTTGTGGAACACTACTGATTGCCGCGAGTATTTTCGGGTTTAGAGGAATCTTGGATAAAGTTGATCAGTACTACAAGTACTACAGGGCACTAGCATTCATAGGTGGTGCTAACCTTTGCTATTACTTTTGTGTGGCCTTTTACTTTTATAGTAAGGAGGATAACGTACAAAGCAATACATCGAACAAGGACGAGTATCATCAGAATGTGTCCTCAGGTGGTGCGGGTTCTGATGAGAACATGGAAGACGTGAAATCGAACTGTGAATGTTGTAATAATTGTTTTTTGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

524

Amino Acids

59.18

Weight (kDa)

8.59

Isoelectric Point (pI)

39.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 182
AccII CGCG 3 cut(s) 233, 620, 1307
AccIII TCCGGA 1 cut(s) 788
AciI CCGC 5 cut(s) 233, 1041, 1205, 1305, 1505
AclWI GGATC 5 cut(s) 414, 786, 799, 1040, 1215
AcoI YGGCCR 1 cut(s) 377
AcuI CTGAAG 2 cut(s) 64, 483
AcyI GRCGYC 1 cut(s) 573
AfaI GTAC 7 cut(s) 128, 309, 903, 1250, 1352, 1361, 1449
AfiI CCNNNNNNNGG 2 cut(s) 73, 788
AgsI TTSAA 3 cut(s) 156, 436, 811
AhlI ACTAGT 1 cut(s) 898
AjiI CACGTC 1 cut(s) 1531
AjnI CCWGG 1 cut(s) 542
AjuI GAANNNNNNNTTGG 4 cut(s) 128, 160, 1263, 1295
AluBI AGCT 9 cut(s) 33, 90, 170, 344, 560, 686, 836, 966, 998
AluI AGCT 9 cut(s) 33, 90, 170, 344, 560, 686, 836, 966, 998
Alw21I GWGCWC 1 cut(s) 875
AlwI GGATC 5 cut(s) 414, 786, 799, 1040, 1215
Ama87I CYCGRG 3 cut(s) 443, 1120, 1193
Aor13HI TCCGGA 1 cut(s) 788
AoxI GGCC 3 cut(s) 377, 712, 1416
ApeKI GCWGC 6 cut(s) 161, 269, 344, 557, 686, 1038
AspS9I GGNCC 2 cut(s) 239, 712
AsuC2I CCSGG 1 cut(s) 68
AsuHPI GGTGA 3 cut(s) 432, 602, 730
AvaI CYCGRG 3 cut(s) 443, 1120, 1193
AvaII GGWCC 1 cut(s) 239
AxyI CCTNAGG 1 cut(s) 1495
BaeGI GKGCMC 1 cut(s) 1372
BamHI GGATCC 1 cut(s) 791
BarI GAAGNNNNNNTAC 2 cut(s) 641, 673
BbsI GAAGAC 2 cut(s) 70, 1533
Bbv12I GWGCWC 1 cut(s) 875
BbvI GCAGC 6 cut(s) 148, 256, 331, 569, 673, 1025
BccI CCATC 8 cut(s) 115, 206, 546, 616, 623, 734, 1178, 1211
BceAI ACGGC 2 cut(s) 392, 914
BcgI CGANNNNNNTGC 2 cut(s) 1184, 1218
BciT130I CCWGG 1 cut(s) 544
BclI TGATCA 1 cut(s) 1345
BcnI CCSGG 1 cut(s) 68
BcuI ACTAGT 1 cut(s) 898
BfaI CTAG 4 cut(s) 899, 995, 1245, 1373
BfmI CTRYAG 3 cut(s) 165, 687, 1363
BglI GCCNNNNNGGC 1 cut(s) 122
BglII AGATCT 1 cut(s) 584
BisI GCNGC 7 cut(s) 162, 270, 345, 558, 687, 1039, 1305
BlpI GCTNAGC 1 cut(s) 832
BlsI GCNGC 7 cut(s) 163, 271, 346, 559, 688, 1040, 1306
BmcAI AGTACT 3 cut(s) 903, 1352, 1361
Bme1390I CCNGG 2 cut(s) 68, 544
Bme18I GGWCC 1 cut(s) 239
BmeT110I CYCGRG 3 cut(s) 443, 1120, 1193
BmgBI CACGTC 1 cut(s) 1531
BmgT120I GGNCC 2 cut(s) 239, 712
BmiI GGNNCC 3 cut(s) 150, 536, 793
BmrFI CCNGG 2 cut(s) 68, 544
BmsI GCATC 2 cut(s) 72, 663
BpiI GAAGAC 2 cut(s) 70, 1533
BpmI CTGGAG 1 cut(s) 1006
Bpu1102I GCTNAGC 1 cut(s) 832
BpuEI CTTGAG 1 cut(s) 422
BpuMI CCSGG 1 cut(s) 68
BsaAI YACGTR 2 cut(s) 130, 419
BsaHI GRCGYC 1 cut(s) 573
BsaJI CCNNGG 6 cut(s) 48, 67, 468, 542, 1179, 1279
BsaWI WCCGGW 1 cut(s) 788
Bsc4I CCNNNNNNNGG 2 cut(s) 73, 788
Bse118I RCCGGY 1 cut(s) 379
Bse1I ACTGG 1 cut(s) 1266
Bse21I CCTNAGG 1 cut(s) 1495
Bse3DI GCAATG 2 cut(s) 213, 1228
BseAI TCCGGA 1 cut(s) 788
BseBI CCWGG 1 cut(s) 544
BseDI CCNNGG 6 cut(s) 48, 67, 468, 542, 1179, 1279
BseGI GGATG 4 cut(s) 63, 194, 538, 1189
BseLI CCNNNNNNNGG 2 cut(s) 73, 788
BseMI GCAATG 2 cut(s) 213, 1228
BseMII CTCAG 1 cut(s) 1509
BseNI ACTGG 1 cut(s) 1266
BseSI GKGCMC 1 cut(s) 1372
BseX3I CGGCCG 1 cut(s) 377
BseXI GCAGC 6 cut(s) 148, 256, 331, 569, 673, 1025
BseYI CCCAGC 2 cut(s) 682, 925
BsgI GTGCAG 2 cut(s) 152, 576
Bsh1236I CGCG 3 cut(s) 233, 620, 1307
Bsh1285I CGRYCG 1 cut(s) 380
BshFI GGCC 3 cut(s) 379, 714, 1418
BsiEI CGRYCG 1 cut(s) 380
BsiHKAI GWGCWC 1 cut(s) 875
BsiHKCI CYCGRG 3 cut(s) 443, 1120, 1193
BsiSI CCGG 3 cut(s) 67, 380, 789
BsiWI CGTACG 1 cut(s) 126
BslI CCNNNNNNNGG 2 cut(s) 73, 788
BsmI GAATGC 1 cut(s) 1376
BsnI GGCC 3 cut(s) 379, 714, 1418
BsoBI CYCGRG 3 cut(s) 443, 1120, 1193
Bsp1286I GDGCHC 2 cut(s) 875, 1372
Bsp13I TCCGGA 1 cut(s) 788
Bsp143I GATC 8 cut(s) 406, 475, 584, 706, 791, 1032, 1207, 1345
Bsp1720I GCTNAGC 1 cut(s) 832
Bsp19I CCATGG 1 cut(s) 1179
Bsp68I TCGCGA 1 cut(s) 620
BspACI CCGC 5 cut(s) 233, 1041, 1205, 1305, 1505
BspANI GGCC 3 cut(s) 379, 714, 1418
BspCNI CTCAG 1 cut(s) 1508
BspEI TCCGGA 1 cut(s) 788
BspFNI CGCG 3 cut(s) 233, 620, 1307
BspLI GGNNCC 3 cut(s) 150, 536, 793
BspMAI CTGCAG 1 cut(s) 691
BspPI GGATC 5 cut(s) 414, 786, 799, 1040, 1215
BsrDI GCAATG 2 cut(s) 213, 1228
BsrFI RCCGGY 1 cut(s) 379
BsrI ACTGG 1 cut(s) 1266
BssAI RCCGGY 1 cut(s) 379
BssECI CCNNGG 6 cut(s) 48, 67, 468, 542, 1179, 1279
BssMI GATC 8 cut(s) 406, 475, 584, 706, 791, 1032, 1207, 1345
BssNI GRCGYC 1 cut(s) 573
BssT1I CCWWGG 3 cut(s) 48, 1179, 1279
Bst2UI CCWGG 1 cut(s) 544
Bst4CI ACNGT 2 cut(s) 851, 1544
BstACI GRCGYC 1 cut(s) 573
BstBAI YACGTR 2 cut(s) 130, 419
BstC8I GCNNGC 2 cut(s) 31, 381
BstDEI CTNAG 3 cut(s) 550, 832, 1495
BstDSI CCRYGG 1 cut(s) 1179
BstF5I GGATG 4 cut(s) 63, 194, 538, 1189
BstFNI CGCG 3 cut(s) 233, 620, 1307
BstKTI GATC 8 cut(s) 409, 478, 587, 709, 794, 1035, 1210, 1348
BstMBI GATC 8 cut(s) 406, 475, 584, 706, 791, 1032, 1207, 1345
BstMCI CGRYCG 1 cut(s) 380
BstMWI GCNNNNNNNGC 4 cut(s) 122, 167, 692, 842
BstNI CCWGG 1 cut(s) 544
BstSCI CCNGG 2 cut(s) 66, 542
BstSFI CTRYAG 3 cut(s) 165, 687, 1363
BstSLI GKGCMC 1 cut(s) 1372
BstUI CGCG 3 cut(s) 233, 620, 1307
BstV1I GCAGC 6 cut(s) 148, 256, 331, 569, 673, 1025
BstV2I GAAGAC 2 cut(s) 70, 1533
BstX2I RGATCY 2 cut(s) 584, 791
BstXI CCANNNNNNTGG 1 cut(s) 622
BstYI RGATCY 2 cut(s) 584, 791
BstZI CGGCCG 1 cut(s) 377
Bsu36I CCTNAGG 1 cut(s) 1495
BsuRI GGCC 3 cut(s) 379, 714, 1418
BtgI CCRYGG 1 cut(s) 1179
BtgZI GCGATG 3 cut(s) 601, 635, 826
BtrI CACGTC 1 cut(s) 1531
BtsCI GGATG 4 cut(s) 63, 194, 538, 1189
BtsIMutI CAGTG 1 cut(s) 1273
BtuMI TCGCGA 1 cut(s) 620
Cac8I GCNNGC 2 cut(s) 31, 381
Cfr10I RCCGGY 1 cut(s) 379
Cfr13I GGNCC 2 cut(s) 239, 712
CseI GACGC 1 cut(s) 562
Csp6I GTAC 7 cut(s) 127, 308, 902, 1249, 1351, 1360, 1448
CviAII CATG 4 cut(s) 202, 1165, 1180, 1522
CviQI GTAC 7 cut(s) 127, 308, 902, 1249, 1351, 1360, 1448
DdeI CTNAG 3 cut(s) 550, 832, 1495
DpnI GATC 8 cut(s) 408, 477, 586, 708, 793, 1034, 1209, 1347
DpnII GATC 8 cut(s) 406, 475, 584, 706, 791, 1032, 1207, 1345
DraI TTTAAA 1 cut(s) 247
EaeI YGGCCR 1 cut(s) 377
EagI CGGCCG 1 cut(s) 377
EclXI CGGCCG 1 cut(s) 377
Eco130I CCWWGG 3 cut(s) 48, 1179, 1279
Eco32I GATATC 1 cut(s) 303
Eco47I GGWCC 1 cut(s) 239
Eco52I CGGCCG 1 cut(s) 377
Eco57I CTGAAG 2 cut(s) 64, 483
Eco81I CCTNAGG 1 cut(s) 1495
Eco88I CYCGRG 3 cut(s) 443, 1120, 1193
EcoO109I RGGNCCY 1 cut(s) 712
EcoRII CCWGG 1 cut(s) 542
EcoRV GATATC 1 cut(s) 303
EcoT14I CCWWGG 3 cut(s) 48, 1179, 1279
EcoT22I ATGCAT 1 cut(s) 65
ErhI CCWWGG 3 cut(s) 48, 1179, 1279
FaeI CATG 4 cut(s) 205, 1168, 1183, 1525
FatI CATG 4 cut(s) 201, 1164, 1179, 1521
FauI CCCGC 1 cut(s) 1498
FbaI TGATCA 1 cut(s) 1345
Fnu4HI GCNGC 7 cut(s) 162, 270, 345, 558, 687, 1039, 1305
FokI GGATG 4 cut(s) 50, 181, 525, 1196
Fsp4HI GCNGC 7 cut(s) 162, 270, 345, 558, 687, 1039, 1305
FspBI CTAG 4 cut(s) 899, 995, 1245, 1373
GluI GCNGC 7 cut(s) 162, 270, 345, 558, 687, 1039, 1305
GsaI CCCAGC 2 cut(s) 686, 929
GsuI CTGGAG 1 cut(s) 1006
HaeIII GGCC 3 cut(s) 379, 714, 1418
HapII CCGG 3 cut(s) 67, 380, 789
HgaI GACGC 1 cut(s) 562
Hin1I GRCGYC 1 cut(s) 573
Hin1II CATG 4 cut(s) 205, 1168, 1183, 1525
HinfI GANTC 6 cut(s) 317, 735, 1090, 1138, 1212, 1329
HpaII CCGG 3 cut(s) 67, 380, 789
HphI GGTGA 3 cut(s) 432, 602, 730
Hpy166II GTNNAC 2 cut(s) 97, 992
Hpy188I TCNGA 3 cut(s) 322, 1485, 1513
Hpy8I GTNNAC 2 cut(s) 97, 992
HpyAV CCTTC 3 cut(s) 162, 268, 886
HpyCH4III ACNGT 2 cut(s) 851, 1544
HpyCH4IV ACGT 6 cut(s) 129, 418, 523, 1015, 1446, 1530
HpyCH4V TGCA 6 cut(s) 63, 133, 557, 689, 910, 1164
HpyF10VI GCNNNNNNNGC 4 cut(s) 122, 167, 692, 842
HpyF3I CTNAG 3 cut(s) 550, 832, 1495
HpySE526I ACGT 6 cut(s) 129, 418, 523, 1015, 1446, 1530
Hsp92I GRCGYC 1 cut(s) 573
Hsp92II CATG 4 cut(s) 205, 1168, 1183, 1525
Kpn2I TCCGGA 1 cut(s) 788
KroI GCCGGC 1 cut(s) 379
KroNI GCCGGC 1 cut(s) 381
Ksp22I TGATCA 1 cut(s) 1345
Kzo9I GATC 8 cut(s) 406, 475, 584, 706, 791, 1032, 1207, 1345
LmnI GCTCC 5 cut(s) 148, 341, 534, 870, 1003
Lsp1109I GCAGC 6 cut(s) 148, 256, 331, 569, 673, 1025
LweI GCATC 2 cut(s) 72, 663
MaeI CTAG 4 cut(s) 899, 995, 1245, 1373
MaeII ACGT 6 cut(s) 129, 418, 523, 1015, 1446, 1530
MaeIII GTNAC 3 cut(s) 348, 562, 931
MalI GATC 8 cut(s) 408, 477, 586, 708, 793, 1034, 1209, 1347
MboI GATC 8 cut(s) 406, 475, 584, 706, 791, 1032, 1207, 1345
MboII GAAGA 9 cut(s) 70, 485, 509, 512, 515, 518, 649, 764, 1538
MflI RGATCY 2 cut(s) 584, 791
MhlI GDGCHC 2 cut(s) 875, 1372
MluCI AATT 7 cut(s) 19, 288, 599, 891, 1145, 1271, 1558
MlyI GAGTC 2 cut(s) 729, 1221
Mph1103I ATGCAT 1 cut(s) 65
MroI TCCGGA 1 cut(s) 788
MroNI GCCGGC 1 cut(s) 379
MseI TTAA 1 cut(s) 246
MslI CAYNNNNRTG 2 cut(s) 620, 1485
MspA1I CMGCKG 2 cut(s) 560, 686
MspI CCGG 3 cut(s) 67, 380, 789
MspR9I CCNGG 2 cut(s) 68, 544
Mva1269I GAATGC 1 cut(s) 1376
MvaI CCWGG 1 cut(s) 544
MvnI CGCG 3 cut(s) 233, 620, 1307
MwoI GCNNNNNNNGC 4 cut(s) 122, 167, 692, 842
NaeI GCCGGC 1 cut(s) 381
NciI CCSGG 1 cut(s) 68
NcoI CCATGG 1 cut(s) 1179
NdeII GATC 8 cut(s) 406, 475, 584, 706, 791, 1032, 1207, 1345
NgoMIV GCCGGC 1 cut(s) 379
NlaIII CATG 4 cut(s) 205, 1168, 1183, 1525
NlaIV GGNNCC 3 cut(s) 150, 536, 793
NmeAIII GCCGAG 1 cut(s) 493
NmuCI GTSAC 1 cut(s) 931
NruI TCGCGA 1 cut(s) 620
NsiI ATGCAT 1 cut(s) 65
PaeR7I CTCGAG 2 cut(s) 1120, 1193
PcsI WCGNNNNNNNCGW 2 cut(s) 617, 1012
PctI GAATGC 1 cut(s) 1376
PdiI GCCGGC 1 cut(s) 381
PfeI GAWTC 4 cut(s) 317, 1090, 1138, 1329
Pfl23II CGTACG 1 cut(s) 126
PkrI GCNGC 7 cut(s) 163, 271, 346, 559, 688, 1040, 1306
PleI GAGTC 2 cut(s) 729, 1220
PpsI GAGTC 2 cut(s) 729, 1220
Ppu21I YACGTR 2 cut(s) 130, 419
PsiI TTATAA 1 cut(s) 182
Psp6I CCWGG 1 cut(s) 542
PspFI CCCAGC 2 cut(s) 682, 925
PspGI CCWGG 1 cut(s) 542
PspLI CGTACG 1 cut(s) 126
PspN4I GGNNCC 3 cut(s) 150, 536, 793
PspPI GGNCC 2 cut(s) 239, 712
PspXI VCTCGAGB 2 cut(s) 1120, 1193
PstI CTGCAG 1 cut(s) 691
PsuI RGATCY 2 cut(s) 584, 791
PvuII CAGCTG 2 cut(s) 560, 686
RruI TCGCGA 1 cut(s) 620
RsaI GTAC 7 cut(s) 128, 309, 903, 1250, 1352, 1361, 1449
RsaNI GTAC 7 cut(s) 127, 308, 902, 1249, 1351, 1360, 1448
RseI CAYNNNNRTG 2 cut(s) 620, 1485
SaqAI TTAA 1 cut(s) 246
SatI GCNGC 7 cut(s) 162, 270, 345, 558, 687, 1039, 1305
Sau3AI GATC 8 cut(s) 406, 475, 584, 706, 791, 1032, 1207, 1345
Sau96I GGNCC 2 cut(s) 239, 712
ScaI AGTACT 3 cut(s) 903, 1352, 1361
SchI GAGTC 2 cut(s) 729, 1221
ScrFI CCNGG 2 cut(s) 68, 544
SduI GDGCHC 2 cut(s) 875, 1372
SfaNI GCATC 2 cut(s) 72, 663
SfcI CTRYAG 3 cut(s) 165, 687, 1363
Sfr274I CTCGAG 2 cut(s) 1120, 1193
SinI GGWCC 1 cut(s) 239
SlaI CTCGAG 2 cut(s) 1120, 1193
SmiMI CAYNNNNRTG 2 cut(s) 620, 1485
SmlI CTYRAG 3 cut(s) 401, 1120, 1193
SmoI CTYRAG 3 cut(s) 401, 1120, 1193
SpeI ACTAGT 1 cut(s) 898
Sse9I AATT 7 cut(s) 19, 288, 599, 891, 1145, 1271, 1558
SsiI CCGC 5 cut(s) 233, 1041, 1205, 1305, 1505
SspI AATATT 5 cut(s) 139, 178, 333, 413, 1114
SspMI CTAG 4 cut(s) 899, 995, 1245, 1373
StyD4I CCNGG 2 cut(s) 66, 542
StyI CCWWGG 3 cut(s) 48, 1179, 1279
TaaI ACNGT 2 cut(s) 851, 1544
TaiI ACGT 6 cut(s) 132, 421, 526, 1018, 1449, 1533
TaqI TCGA 9 cut(s) 611, 858, 968, 1121, 1194, 1210, 1215, 1463, 1538
TaqII GACCGA 1 cut(s) 996
TasI AATT 7 cut(s) 19, 288, 599, 891, 1145, 1271, 1558
TatI WGTACW 4 cut(s) 307, 901, 1350, 1359
TauI GCSGC 1 cut(s) 1307
TfiI GAWTC 4 cut(s) 317, 1090, 1138, 1329
Tru1I TTAA 1 cut(s) 246
Tru9I TTAA 1 cut(s) 246
TscAI CASTG 1 cut(s) 1273
TseFI GTSAC 1 cut(s) 931
TseI GCWGC 6 cut(s) 161, 269, 344, 557, 686, 1038
Tsp45I GTSAC 1 cut(s) 931
TspDTI ATGAA 4 cut(s) 202, 829, 1082, 1369
TspRI CASTG 1 cut(s) 1273
VpaK11BI GGWCC 1 cut(s) 239
XhoI CTCGAG 2 cut(s) 1120, 1193
XspI CTAG 4 cut(s) 899, 995, 1245, 1373
ZrmI AGTACT 3 cut(s) 903, 1352, 1361
Zsp2I ATGCAT 1 cut(s) 65
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.