RLG00000007576

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr2
OG0000950
Physical Location & Seq
Forward (+)
18453554 .. 18456711
3158 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000007576

Sequence Viewer

Length: 1725 bp
ATGAGAAAGTGGATCGGAAACTACCTCTACTTTTCCAAGGCTACTTTATGCATCCGGGGTTTGGTCTTGAGCCATAGCTTCGTTAACCATGTGGTAATATCTGTACTGATAACGTACCTCACGGTTGAAATTTGGAAGCCATCAAATGATAATTATCAGCTGGCTGCTGTAGCTATAAATATTCTAGATGGGGTATCATCCATCATGGTGTGTGTCCTTGCTTTCATCTCAAACAGGGTCGGTCCTTTTAAGGTGATTGCCTCCACGAATGCTGCCTATATTCTTGGCCTGGTTCTATTGTGGAGTTCATCCCCGAAAGCCAATGCATCTGATAAAGTATTCTACGGAGCTGGCCTACTGTTAGCATTAGGCGAGGCTGGACGGCCGGCTGCTTTGGGTAAATTTCTCGAGGACCAATATTTAAGTGAAGCTGACCACAAGATCAAATTACCCGAAACAAAAAGTATTGAAGAAGAAGATCATATTTCGACCAGAAACGAAGAACAAGAAGATCATTTTTCGACCAGAAAAGAAGAAGATGACAAGCAAAAACGAGTGGAAGAGGACAAGCAAAAACGTGTGGAAACCCGTCAAACCGCTTTATGGAGCCATCCCTGGTTCATAGGTGCGGCTGCACCTCTTGCCCTTGTAAGTAGATCTTGGCAAACTCAACTACTGCTCTCAATGATCGCGGTGGGAGTTACTTACTTATTGTTTTGGTATGGCGTCTTCGCGTATTCCAGAAATATCAGAAATGAATCAAATGATCCAGCAGTAGAAGATGCACCAAGAGATCCTGGACTTTGCCAAGCCATCAAAAGTCTCTCACCACCAGAAAAAACAGTAAGCTTGAAGTTTTGGCTCAAACCCAATTCCAGAAAACTTTATTACAAAGAAATGCTAGTCATGTGCTTAGCCTTCATCGTATACAGTCTGGTGGAGGCAACGGGGAGCACCTTCTTTTTCGATCAAATGAGTCTACTGAACTATCACATCGGGAACTTTGAATTGCCCATCATATATTTCGTTATACTCCAATCCTTGTCAAGCTCCGTAATCTCATTTCTCTGGAGGTTACTAGTTCCAAATCAGTCAAAACGTGCTACGTTGGTGAGGATCGGCTGTGGTTTAGTTTGCTCTGTGCTATGTATGGTTGCTGCTTGGCAAGTTGAGATTCGTAGGAAAATTAAGATCAATACGGAATACAGAGGTAAACCCAAACCCGACGAAATTTCCATGAGCATTCTTTGGTTAATTCCACAATTCTCCCTGTTAGGGTTTATGAGAGGGTTAGCAGCAGATGGATTGATCGAGTTTTTTGCGGATCGAGTTGATGGTAATGATAAAAGAAAGGCTAGGTATTATGGATCCTACGCCAGTGAATTAGTCATGGGTATTGGAACACTACTAACCGCCGTTAGTATTCTCGGGTTTAGACGAACTTGGTTGGTTGATGATATATACAAGAATCGTCTGGATAAGTACTACAGGGTACTAGTATTCATAGGTGTTGCTAACCTTTGCTATTACTTCTGTGTCGCCGTTTACTTTTTTACCAAGGAGGATAACTCGCAAGTAGGTGGGGCCGCAGATGGAAATGATCAAAGTGAAATGCCGGAAGAGGACAAAAAATATTATGATCACGAGGTGTCCTCAGGTGGTGCGGAGACTCCGGACTTGGAAGAAATGAAGGCGAGTTTTGATTGTTGTAGTAGTTTCTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PTR2

Protein Analysis

575

Amino Acids

64.95

Weight (kDa)

6.49

Isoelectric Point (pI)

43.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 299 - 443 6.8e-09 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 927, 979
AccII CGCG 2 cut(s) 692, 734
AccIII TCCGGA 1 cut(s) 1672
AciI CCGC 7 cut(s) 597, 629, 692, 1322, 1413, 1587, 1664
AclWI GGATC 7 cut(s) 20, 761, 788, 1124, 1332, 1362, 1375
AcoI YGGCCR 1 cut(s) 383
AcsI RAATTY 3 cut(s) 129, 401, 1230
AcyI GRCGYC 1 cut(s) 726
AdeI CACNNNGTG 1 cut(s) 1648
AfaI GTAC 4 cut(s) 105, 116, 1484, 1494
AfiI CCNNNNNNNGG 3 cut(s) 61, 603, 1275
AflIII ACRYGT 1 cut(s) 577
AgsI TTSAA 4 cut(s) 128, 470, 853, 1007
AhlI ACTAGT 2 cut(s) 1078, 1495
AjnI CCWGG 3 cut(s) 288, 614, 796
AluBI AGCT 7 cut(s) 78, 160, 173, 350, 431, 849, 1050
AluI AGCT 7 cut(s) 78, 160, 173, 350, 431, 849, 1050
Alw21I GWGCWC 1 cut(s) 956
Alw26I GTCTC 2 cut(s) 827, 1661
AlwI GGATC 7 cut(s) 20, 761, 788, 1124, 1332, 1362, 1375
Ama87I CYCGRG 2 cut(s) 407, 1427
Aor13HI TCCGGA 1 cut(s) 1672
AoxI GGCC 4 cut(s) 286, 352, 383, 1584
ApeKI GCWGC 6 cut(s) 164, 272, 389, 632, 1157, 1295
ApoI RAATTY 3 cut(s) 129, 401, 1230
AspS9I GGNCC 3 cut(s) 242, 412, 1584
AsuC2I CCSGG 1 cut(s) 56
AsuHPI GGTGA 3 cut(s) 265, 819, 1123
AvaI CYCGRG 2 cut(s) 407, 1427
AvaII GGWCC 2 cut(s) 242, 412
AxyI CCTNAGG 1 cut(s) 1654
BamHI GGATCC 1 cut(s) 1367
BarI GAAGNNNNNNTAC 2 cut(s) 713, 745
BauI CACGAG 1 cut(s) 1643
BbsI GAAGAC 1 cut(s) 721
Bbv12I GWGCWC 1 cut(s) 956
BbvI GCAGC 6 cut(s) 151, 259, 376, 619, 1144, 1307
BccI CCATC 9 cut(s) 148, 182, 209, 618, 821, 1022, 1295, 1328, 1586
BceAI ACGGC 3 cut(s) 398, 1400, 1526
BcgI CGANNNNNNTGC 2 cut(s) 1301, 1335
BciT130I CCWGG 3 cut(s) 290, 616, 798
BclI TGATCA 2 cut(s) 1600, 1639
BcnI CCSGG 1 cut(s) 56
BcoDI GTCTC 2 cut(s) 827, 1661
BcuI ACTAGT 2 cut(s) 1078, 1495
BfaI CTAG 5 cut(s) 185, 902, 1079, 1356, 1496
BfmI CTRYAG 2 cut(s) 168, 1486
BglII AGATCT 1 cut(s) 656
BisI GCNGC 8 cut(s) 165, 273, 390, 630, 633, 1158, 1296, 1587
BlpI GCTNAGC 1 cut(s) 913
BlsI GCNGC 8 cut(s) 166, 274, 391, 631, 634, 1159, 1297, 1588
BmcAI AGTACT 1 cut(s) 1484
Bme1390I CCNGG 4 cut(s) 56, 290, 616, 798
Bme18I GGWCC 2 cut(s) 242, 412
BmeT110I CYCGRG 2 cut(s) 407, 1427
BmgT120I GGNCC 3 cut(s) 242, 412, 1584
BmiI GGNNCC 3 cut(s) 608, 1369, 1585
BmrFI CCNGG 4 cut(s) 56, 290, 616, 798
BmsI GCATC 3 cut(s) 60, 335, 772
BpiI GAAGAC 1 cut(s) 721
BplI GAGNNNNNCTC 4 cut(s) 1553, 1585, 1637, 1669
BpmI CTGGAG 1 cut(s) 1090
Bpu1102I GCTNAGC 1 cut(s) 913
BpuEI CTTGAG 1 cut(s) 88
BpuMI CCSGG 1 cut(s) 56
BsaBI GATNNNNATC 1 cut(s) 153
BsaHI GRCGYC 1 cut(s) 726
BsaJI CCNNGG 4 cut(s) 36, 55, 614, 1557
BsaWI WCCGGW 1 cut(s) 1672
Bsc4I CCNNNNNNNGG 3 cut(s) 61, 603, 1275
Bse118I RCCGGY 1 cut(s) 385
Bse1I ACTGG 1 cut(s) 1377
Bse21I CCTNAGG 1 cut(s) 1654
Bse8I GATNNNNATC 1 cut(s) 153
BseAI TCCGGA 1 cut(s) 1672
BseBI CCWGG 3 cut(s) 290, 616, 798
BseDI CCNNGG 4 cut(s) 36, 55, 614, 1557
BseGI GGATG 4 cut(s) 51, 197, 308, 610
BseJI GATNNNNATC 1 cut(s) 153
BseLI CCNNNNNNNGG 3 cut(s) 61, 603, 1275
BseMII CTCAG 1 cut(s) 1668
BseNI ACTGG 1 cut(s) 1377
BseX3I CGGCCG 1 cut(s) 383
BseXI GCAGC 6 cut(s) 151, 259, 376, 619, 1144, 1307
BsgI GTGCAG 1 cut(s) 618
Bsh1236I CGCG 2 cut(s) 692, 734
Bsh1285I CGRYCG 1 cut(s) 386
BshFI GGCC 4 cut(s) 288, 354, 385, 1586
BsiEI CGRYCG 1 cut(s) 386
BsiHKAI GWGCWC 1 cut(s) 956
BsiHKCI CYCGRG 2 cut(s) 407, 1427
BsiSI CCGG 4 cut(s) 55, 386, 1616, 1673
BslI CCNNNNNNNGG 3 cut(s) 61, 603, 1275
BsmAI GTCTC 2 cut(s) 827, 1661
BsmI GAATGC 2 cut(s) 274, 1242
BsnI GGCC 4 cut(s) 288, 354, 385, 1586
BsoBI CYCGRG 2 cut(s) 407, 1427
Bsp1286I GDGCHC 1 cut(s) 956
Bsp13I TCCGGA 1 cut(s) 1672
Bsp1720I GCTNAGC 1 cut(s) 913
BspACI CCGC 7 cut(s) 597, 629, 692, 1322, 1413, 1587, 1664
BspANI GGCC 4 cut(s) 288, 354, 385, 1586
BspCNI CTCAG 1 cut(s) 1667
BspEI TCCGGA 1 cut(s) 1672
BspFNI CGCG 2 cut(s) 692, 734
BspLI GGNNCC 3 cut(s) 608, 1369, 1585
BspPI GGATC 7 cut(s) 20, 761, 788, 1124, 1332, 1362, 1375
BsrFI RCCGGY 1 cut(s) 385
BsrI ACTGG 1 cut(s) 1377
BssAI RCCGGY 1 cut(s) 385
BssECI CCNNGG 4 cut(s) 36, 55, 614, 1557
BssNAI GTATAC 1 cut(s) 928
BssNI GRCGYC 1 cut(s) 726
BssSI CACGAG 1 cut(s) 1643
BssT1I CCWWGG 2 cut(s) 36, 1557
Bst1107I GTATAC 1 cut(s) 928
Bst2BI CACGAG 1 cut(s) 1643
Bst2UI CCWGG 3 cut(s) 290, 616, 798
Bst4CI ACNGT 4 cut(s) 124, 360, 844, 932
Bst6I CTCTTC 2 cut(s) 555, 1614
BstACI GRCGYC 1 cut(s) 726
BstAPI GCANNNNNTGC 1 cut(s) 641
BstC8I GCNNGC 3 cut(s) 162, 352, 387
BstDEI CTNAG 2 cut(s) 913, 1654
BstF5I GGATG 4 cut(s) 51, 197, 308, 610
BstFNI CGCG 2 cut(s) 692, 734
BstMAI GTCTC 2 cut(s) 827, 1661
BstMCI CGRYCG 1 cut(s) 386
BstMWI GCNNNNNNNGC 2 cut(s) 170, 641
BstNI CCWGG 3 cut(s) 290, 616, 798
BstSCI CCNGG 4 cut(s) 54, 288, 614, 796
BstSFI CTRYAG 2 cut(s) 168, 1486
BstUI CGCG 2 cut(s) 692, 734
BstV1I GCAGC 6 cut(s) 151, 259, 376, 619, 1144, 1307
BstV2I GAAGAC 1 cut(s) 721
BstX2I RGATCY 3 cut(s) 656, 793, 1367
BstYI RGATCY 3 cut(s) 656, 793, 1367
BstZ17I GTATAC 1 cut(s) 928
BstZI CGGCCG 1 cut(s) 383
Bsu36I CCTNAGG 1 cut(s) 1654
BsuRI GGCC 4 cut(s) 288, 354, 385, 1586
BtsCI GGATG 4 cut(s) 51, 197, 308, 610
BtsIMutI CAGTG 1 cut(s) 1384
Cac8I GCNNGC 3 cut(s) 162, 352, 387
Cfr10I RCCGGY 1 cut(s) 385
Cfr13I GGNCC 3 cut(s) 242, 412, 1584
CseI GACGC 1 cut(s) 715
Csp6I GTAC 4 cut(s) 104, 115, 1483, 1493
CviAII CATG 5 cut(s) 89, 205, 907, 1237, 1390
CviQI GTAC 4 cut(s) 104, 115, 1483, 1493
DdeI CTNAG 2 cut(s) 913, 1654
DraIII CACNNNGTG 1 cut(s) 1648
EaeI YGGCCR 1 cut(s) 383
EagI CGGCCG 1 cut(s) 383
Eam1104I CTCTTC 2 cut(s) 555, 1614
EarI CTCTTC 2 cut(s) 555, 1614
EclXI CGGCCG 1 cut(s) 383
Eco130I CCWWGG 2 cut(s) 36, 1557
Eco47I GGWCC 2 cut(s) 242, 412
Eco52I CGGCCG 1 cut(s) 383
Eco81I CCTNAGG 1 cut(s) 1654
Eco88I CYCGRG 2 cut(s) 407, 1427
EcoRII CCWGG 3 cut(s) 288, 614, 796
EcoT14I CCWWGG 2 cut(s) 36, 1557
EcoT22I ATGCAT 2 cut(s) 53, 328
ErhI CCWWGG 2 cut(s) 36, 1557
FaeI CATG 5 cut(s) 92, 208, 910, 1240, 1393
FalI AAGNNNNNCTT 2 cut(s) 643, 675
FatI CATG 5 cut(s) 88, 204, 906, 1236, 1389
FbaI TGATCA 2 cut(s) 1600, 1639
FblI GTMKAC 2 cut(s) 927, 979
Fnu4HI GCNGC 8 cut(s) 165, 273, 390, 630, 633, 1158, 1296, 1587
FokI GGATG 4 cut(s) 38, 184, 295, 597
Fsp4HI GCNGC 8 cut(s) 165, 273, 390, 630, 633, 1158, 1296, 1587
FspBI CTAG 5 cut(s) 185, 902, 1079, 1356, 1496
GluI GCNGC 8 cut(s) 165, 273, 390, 630, 633, 1158, 1296, 1587
GsuI CTGGAG 1 cut(s) 1090
HaeIII GGCC 4 cut(s) 288, 354, 385, 1586
HapII CCGG 4 cut(s) 55, 386, 1616, 1673
HgaI GACGC 1 cut(s) 715
Hin1I GRCGYC 1 cut(s) 726
Hin1II CATG 5 cut(s) 92, 208, 910, 1240, 1393
HincII GTYRAC 1 cut(s) 85
HindII GTYRAC 1 cut(s) 85
HindIII AAGCTT 1 cut(s) 847
HinfI GANTC 5 cut(s) 758, 976, 1174, 1468, 1669
HpaI GTTAAC 1 cut(s) 85
HpaII CCGG 4 cut(s) 55, 386, 1616, 1673
HphI GGTGA 3 cut(s) 265, 819, 1123
Hpy166II GTNNAC 5 cut(s) 85, 928, 980, 1214, 1546
Hpy188I TCNGA 3 cut(s) 17, 331, 752
Hpy8I GTNNAC 5 cut(s) 85, 928, 980, 1214, 1546
Hpy99I CGWCG 1 cut(s) 1229
HpyAV CCTTC 3 cut(s) 928, 967, 1684
HpyCH4III ACNGT 4 cut(s) 124, 360, 844, 932
HpyCH4IV ACGT 4 cut(s) 113, 577, 1099, 1106
HpyCH4V TGCA 4 cut(s) 51, 326, 635, 785
HpyF10VI GCNNNNNNNGC 2 cut(s) 170, 641
HpyF3I CTNAG 2 cut(s) 913, 1654
HpySE526I ACGT 4 cut(s) 113, 577, 1099, 1106
Hsp92I GRCGYC 1 cut(s) 726
Hsp92II CATG 5 cut(s) 92, 208, 910, 1240, 1393
Kpn2I TCCGGA 1 cut(s) 1672
KroI GCCGGC 1 cut(s) 385
KroNI GCCGGC 1 cut(s) 387
Ksp22I TGATCA 2 cut(s) 1600, 1639
KspAI GTTAAC 1 cut(s) 85
LmnI GCTCC 4 cut(s) 347, 606, 951, 1055
Lsp1109I GCAGC 6 cut(s) 151, 259, 376, 619, 1144, 1307
LweI GCATC 3 cut(s) 60, 335, 772
MaeI CTAG 5 cut(s) 185, 902, 1079, 1356, 1496
MaeII ACGT 4 cut(s) 113, 577, 1099, 1106
MaeIII GTNAC 2 cut(s) 700, 1074
MflI RGATCY 3 cut(s) 656, 793, 1367
MhlI GDGCHC 1 cut(s) 956
MlyI GAGTC 2 cut(s) 985, 1663
Mph1103I ATGCAT 2 cut(s) 53, 328
MroI TCCGGA 1 cut(s) 1672
MroNI GCCGGC 1 cut(s) 385
MseI TTAA 5 cut(s) 84, 249, 422, 1188, 1253
MslI CAYNNNNRTG 1 cut(s) 206
MspA1I CMGCKG 1 cut(s) 160
MspI CCGG 4 cut(s) 55, 386, 1616, 1673
MspR9I CCNGG 4 cut(s) 56, 290, 616, 798
Mva1269I GAATGC 2 cut(s) 274, 1242
MvaI CCWGG 3 cut(s) 290, 616, 798
MvnI CGCG 2 cut(s) 692, 734
MwoI GCNNNNNNNGC 2 cut(s) 170, 641
NaeI GCCGGC 1 cut(s) 387
NciI CCSGG 1 cut(s) 56
NgoMIV GCCGGC 1 cut(s) 385
NlaIII CATG 5 cut(s) 92, 208, 910, 1240, 1393
NlaIV GGNNCC 3 cut(s) 608, 1369, 1585
NsiI ATGCAT 2 cut(s) 53, 328
PaeR7I CTCGAG 1 cut(s) 407
PctI GAATGC 2 cut(s) 274, 1242
PdiI GCCGGC 1 cut(s) 387
PfeI GAWTC 3 cut(s) 758, 1174, 1468
PfoI TCCNGGA 1 cut(s) 796
PkrI GCNGC 8 cut(s) 166, 274, 391, 631, 634, 1159, 1297, 1588
PleI GAGTC 2 cut(s) 984, 1663
PpsI GAGTC 2 cut(s) 984, 1663
Psp6I CCWGG 3 cut(s) 288, 614, 796
PspGI CCWGG 3 cut(s) 288, 614, 796
PspN4I GGNNCC 3 cut(s) 608, 1369, 1585
PspPI GGNCC 3 cut(s) 242, 412, 1584
PsuI RGATCY 3 cut(s) 656, 793, 1367
PvuII CAGCTG 1 cut(s) 160
RsaI GTAC 4 cut(s) 105, 116, 1484, 1494
RsaNI GTAC 4 cut(s) 104, 115, 1483, 1493
RseI CAYNNNNRTG 1 cut(s) 206
SaqAI TTAA 5 cut(s) 84, 249, 422, 1188, 1253
SatI GCNGC 8 cut(s) 165, 273, 390, 630, 633, 1158, 1296, 1587
Sau96I GGNCC 3 cut(s) 242, 412, 1584
ScaI AGTACT 1 cut(s) 1484
SchI GAGTC 2 cut(s) 985, 1663
ScrFI CCNGG 4 cut(s) 56, 290, 616, 798
SduI GDGCHC 1 cut(s) 956
SfaNI GCATC 3 cut(s) 60, 335, 772
SfcI CTRYAG 2 cut(s) 168, 1486
Sfr274I CTCGAG 1 cut(s) 407
SinI GGWCC 2 cut(s) 242, 412
SlaI CTCGAG 1 cut(s) 407
SmiMI CAYNNNNRTG 1 cut(s) 206
SmlI CTYRAG 2 cut(s) 67, 407
SmoI CTYRAG 2 cut(s) 67, 407
SpeI ACTAGT 2 cut(s) 1078, 1495
SsiI CCGC 7 cut(s) 597, 629, 692, 1322, 1413, 1587, 1664
SspI AATATT 3 cut(s) 181, 419, 1634
SspMI CTAG 5 cut(s) 185, 902, 1079, 1356, 1496
StyD4I CCNGG 4 cut(s) 54, 288, 614, 796
StyI CCWWGG 2 cut(s) 36, 1557
TaaI ACNGT 4 cut(s) 124, 360, 844, 932
TaiI ACGT 4 cut(s) 116, 580, 1102, 1109
TaqI TCGA 6 cut(s) 408, 488, 521, 966, 1311, 1327
TaqII GACCGA 1 cut(s) 230
TatI WGTACW 2 cut(s) 103, 1482
TauI GCSGC 2 cut(s) 632, 1589
TfiI GAWTC 3 cut(s) 758, 1174, 1468
Tru1I TTAA 5 cut(s) 84, 249, 422, 1188, 1253
Tru9I TTAA 5 cut(s) 84, 249, 422, 1188, 1253
TscAI CASTG 1 cut(s) 1384
TseI GCWGC 6 cut(s) 164, 272, 389, 632, 1157, 1295
TspDTI ATGAA 7 cut(s) 214, 297, 610, 771, 910, 1492, 1703
TspGWI ACGGA 3 cut(s) 360, 1042, 1214
TspRI CASTG 1 cut(s) 1384
VpaK11BI GGWCC 2 cut(s) 242, 412
XapI RAATTY 3 cut(s) 129, 401, 1230
XbaI TCTAGA 1 cut(s) 184
XhoI CTCGAG 1 cut(s) 407
XmiI GTMKAC 2 cut(s) 927, 979
XspI CTAG 5 cut(s) 185, 902, 1079, 1356, 1496
ZrmI AGTACT 1 cut(s) 1484
Zsp2I ATGCAT 2 cut(s) 53, 328
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.