Rmu_sc0002509.1_g000023

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0002509.1
OG0000950
Physical Location & Seq
Reverse (-)
117583 .. 120533
2951 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0002509.1_g000023.1.cds

Sequence Viewer

Length: 1878 bp
atgttgcagggactaattttaatctgggtgttcgacgaacccatactagcatgtggagctgcgttacttgtagcattaggcgaggctggacggccagcttctttgggtagatttcttgaggatcaatattcacttgaaactgacgacttcaaagcacccgaaaaacaaaataatcctgaagccgagaaagatcatactttgaccagaaaagaagaagatgatccttttcaaaaagaagaaaaagaagatgataagcgaaaatgtgctttgtggagccatccctggtttttaggtgcagctgtaactcttggctttataaagaggtctgatggaactcaattactgctttcgaccatcgccatgggagttggttactcactgttctggtatggcgtcttctcatattccagaaataatagtccagctgcagaagcaacacatagagatcagggccttttccaagccatcaaaagtctctctccaccagaaaaagaagcaaggtgcagattttcgctgaaactcaaatccggatccagaaaacgttattacaaagaaatgttagttatgtgctcagctttcatcgcatacagtctgatcgagacaacggggagcaccttctttttcgcccaaattacacgagaactgcataacagggaaatcccagctgtcacttatttcgttatcctccaatccttgtcaagctccataacctcatttttctggagtttactagctccaaatcggtcaaaacgtgcaacattgttcaggatcgggtgcggattggtttgttctgtgctatgtatggttgttgcttggcgagttgcgattcataaaagtaaagactatattgacgcgaatcataaaagtaaagactatattgacgagaatcatacaagtaaagaacatatttactggagcctcgtttggttcattccacaattctgcctgttagggtgcatgagagggtttgccatagttggattactcgtgttttttgcggatcaagtcgatggcaatgataatgggaaaagaaatgctaggtactatggaacttatgccagtgaattagtccttggtagtggaacactactgattgccgcgagtattttcgggtttagaggaatcttggatgacgttcatgagtactacaagtactacagggcactagcattcataggtggtgctaacctttgctattacttttgtgtggccttttacttttataccaaggaggataacgtacaaagcggtacatcgaacaaggacgagtatcatgtgtcctcaggtggtgcgggttttgatgagaacaaggatgctatcctccccaacttgaaagagttggatctatatgaatgtcgcagtttagttgaggttcctgattccgttggattccttcggaacctttttaccttgaatgttgacggctgctctaaccttattatgtttccaagaaaaatcaactggaaatatgtagaagctatttctatccactcttgcaagcttgaggaattttcagaagttgggaaagagatgggttccttgagaattttggatttatcagacacttgcattaaagaattgggtccgtccattacaaatctcactgggctcgaagagttgatcctaaggaactgtcaaaatcttacaactctgccgcgtagcatttatgagctgcgcaatctacaagttcttgatgcgaggcagtgcccaaaacttgctgcatttccagaaattccaataaagatggatttcttgagagaactttatttaggaggcggtgccatcagagaattggatgagtcaattggaaatctcattgggcttcaaacgttggatcttacgaatgttagaaaacctgatgaacaagacattacatag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

625

Amino Acids

70.67

Weight (kDa)

5.72

Isoelectric Point (pI)

39.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 317
Acc16I TGCGCA 1 cut(s) 1676
AccB1I GGYRCC 1 cut(s) 1778
AccII CGCG 3 cut(s) 854, 1100, 1657
AccIII TCCGGA 1 cut(s) 527
AciI CCGC 7 cut(s) 777, 998, 1098, 1248, 1292, 1655, 1776
AclI AACGTT 2 cut(s) 541, 1829
AclWI GGATC 9 cut(s) 129, 215, 525, 538, 776, 1008, 1350, 1615, 1842
AcoI YGGCCR 1 cut(s) 92
AcsI RAATTY 3 cut(s) 1508, 1545, 1731
AcuI CTGAAG 1 cut(s) 198
AcyI GRCGYC 1 cut(s) 393
AfaI GTAC 5 cut(s) 1043, 1145, 1154, 1242, 1252
AgsI TTSAA 6 cut(s) 137, 151, 230, 1333, 1414, 1826
AjnI CCWGG 1 cut(s) 281
AjuI GAANNNNNNNTTGG 2 cut(s) 1056, 1088
Alw21I GWGCWC 2 cut(s) 572, 614
Alw26I GTCTC 2 cut(s) 479, 593
AlwI GGATC 9 cut(s) 129, 215, 525, 538, 776, 1008, 1350, 1615, 1842
Aor13HI TCCGGA 1 cut(s) 527
AoxI GGCC 3 cut(s) 92, 451, 1209
ApeKI GCWGC 6 cut(s) 59, 296, 425, 1425, 1672, 1718
ApoI RAATTY 3 cut(s) 1508, 1545, 1731
AspLEI GCGC 1 cut(s) 1677
AspS9I GGNCC 2 cut(s) 451, 1583
AvaII GGWCC 1 cut(s) 1583
AxyI CCTNAGG 2 cut(s) 1282, 1625
BaeGI GKGCMC 2 cut(s) 1165, 1709
BamHI GGATCC 1 cut(s) 530
BanI GGYRCC 1 cut(s) 1778
BanII GRGCYC 1 cut(s) 1611
BarI GAAGNNNNNNTAC 2 cut(s) 380, 412
BauI CACGAG 2 cut(s) 636, 986
BbsI GAAGAC 1 cut(s) 388
Bbv12I GWGCWC 2 cut(s) 572, 614
BbvI GCAGC 6 cut(s) 46, 308, 412, 1412, 1659, 1705
BccI CCATC 8 cut(s) 285, 323, 362, 473, 1004, 1525, 1738, 1790
BceAI ACGGC 2 cut(s) 107, 1438
BciT130I CCWGG 1 cut(s) 283
BcoDI GTCTC 2 cut(s) 479, 593
BfaI CTAG 4 cut(s) 47, 731, 1038, 1166
BfmI CTRYAG 2 cut(s) 426, 1156
BisI GCNGC 8 cut(s) 60, 297, 426, 1098, 1426, 1655, 1673, 1719
BlpI GCTNAGC 1 cut(s) 571
BlsI GCNGC 8 cut(s) 61, 298, 427, 1099, 1427, 1656, 1674, 1720
BmcAI AGTACT 2 cut(s) 1145, 1154
Bme1390I CCNGG 1 cut(s) 283
Bme18I GGWCC 1 cut(s) 1583
BmgT120I GGNCC 2 cut(s) 451, 1583
BmiI GGNNCC 8 cut(s) 275, 532, 917, 1374, 1400, 1537, 1584, 1780
BmrFI CCNGG 1 cut(s) 283
BmrI ACTGGG 1 cut(s) 1614
BmsI GCATC 2 cut(s) 1303, 1684
BmuI ACTGGG 1 cut(s) 1614
BpiI GAAGAC 1 cut(s) 388
BpmI CTGGAG 2 cut(s) 742, 934
Bpu1102I GCTNAGC 1 cut(s) 571
BpuEI CTTGAG 4 cut(s) 137, 1523, 1561, 1774
BsaHI GRCGYC 1 cut(s) 393
BsaJI CCNNGG 4 cut(s) 281, 360, 1072, 1227
BsaWI WCCGGW 1 cut(s) 527
Bse1I ACTGG 4 cut(s) 917, 1059, 1466, 1609
Bse21I CCTNAGG 2 cut(s) 1282, 1625
Bse3DI GCAATG 1 cut(s) 1021
BseAI TCCGGA 1 cut(s) 527
BseBI CCWGG 1 cut(s) 283
BseDI CCNNGG 4 cut(s) 281, 360, 1072, 1227
BseGI GGATG 4 cut(s) 277, 1135, 1318, 1801
BseMI GCAATG 1 cut(s) 1021
BseMII CTCAG 2 cut(s) 585, 1296
BseNI ACTGG 4 cut(s) 917, 1059, 1466, 1609
BseSI GKGCMC 2 cut(s) 1165, 1709
BseXI GCAGC 6 cut(s) 46, 308, 412, 1412, 1659, 1705
BseYI CCCAGC 1 cut(s) 661
BsgI GTGCAG 2 cut(s) 315, 523
Bsh1236I CGCG 3 cut(s) 854, 1100, 1657
BshFI GGCC 3 cut(s) 94, 453, 1211
BshNI GGYRCC 1 cut(s) 1778
BsiHKAI GWGCWC 2 cut(s) 572, 614
BsiSI CCGG 1 cut(s) 528
BslFI GGGAC 1 cut(s) 24
BsmAI GTCTC 2 cut(s) 479, 593
BsmFI GGGAC 1 cut(s) 24
BsmI GAATGC 1 cut(s) 1169
BsnI GGCC 3 cut(s) 94, 453, 1211
Bsp1286I GDGCHC 5 cut(s) 572, 614, 1165, 1611, 1709
Bsp13I TCCGGA 1 cut(s) 527
Bsp1720I GCTNAGC 1 cut(s) 571
Bsp19I CCATGG 1 cut(s) 360
BspACI CCGC 7 cut(s) 777, 998, 1098, 1248, 1292, 1655, 1776
BspANI GGCC 3 cut(s) 94, 453, 1211
BspCNI CTCAG 2 cut(s) 584, 1295
BspEI TCCGGA 1 cut(s) 527
BspFNI CGCG 3 cut(s) 854, 1100, 1657
BspHI TCATGA 1 cut(s) 1138
BspLI GGNNCC 8 cut(s) 275, 532, 917, 1374, 1400, 1537, 1584, 1780
BspMAI CTGCAG 1 cut(s) 430
BspPI GGATC 9 cut(s) 129, 215, 525, 538, 776, 1008, 1350, 1615, 1842
BspT107I GGYRCC 1 cut(s) 1778
BsrDI GCAATG 1 cut(s) 1021
BsrI ACTGG 4 cut(s) 917, 1059, 1466, 1609
BssECI CCNNGG 4 cut(s) 281, 360, 1072, 1227
BssNI GRCGYC 1 cut(s) 393
BssSI CACGAG 2 cut(s) 636, 986
BssT1I CCWWGG 3 cut(s) 360, 1072, 1227
Bst2BI CACGAG 2 cut(s) 636, 986
Bst2UI CCWGG 1 cut(s) 283
Bst4CI ACNGT 3 cut(s) 381, 590, 1634
Bst6I CTCTTC 1 cut(s) 1608
BstACI GRCGYC 1 cut(s) 393
BstC8I GCNNGC 2 cut(s) 96, 1499
BstDEI CTNAG 3 cut(s) 571, 1282, 1625
BstDSI CCRYGG 1 cut(s) 360
BstF5I GGATG 4 cut(s) 277, 1135, 1318, 1801
BstFNI CGCG 3 cut(s) 854, 1100, 1657
BstHHI GCGC 1 cut(s) 1677
BstMAI GTCTC 2 cut(s) 479, 593
BstMWI GCNNNNNNNGC 3 cut(s) 56, 431, 581
BstNI CCWGG 1 cut(s) 283
BstNSI RCATGY 1 cut(s) 54
BstSCI CCNGG 1 cut(s) 281
BstSFI CTRYAG 2 cut(s) 426, 1156
BstSLI GKGCMC 2 cut(s) 1165, 1709
BstUI CGCG 3 cut(s) 854, 1100, 1657
BstV1I GCAGC 6 cut(s) 46, 308, 412, 1412, 1659, 1705
BstV2I GAAGAC 1 cut(s) 388
BstX2I RGATCY 3 cut(s) 530, 1342, 1834
BstXI CCANNNNNNTGG 1 cut(s) 361
BstYI RGATCY 3 cut(s) 530, 1342, 1834
Bsu36I CCTNAGG 2 cut(s) 1282, 1625
BsuRI GGCC 3 cut(s) 94, 453, 1211
BtgI CCRYGG 1 cut(s) 360
BtgZI GCGATG 2 cut(s) 340, 565
BtsCI GGATG 4 cut(s) 277, 1135, 1318, 1801
BtsI GCAGTG 1 cut(s) 1709
BtsIMutI CAGTG 4 cut(s) 377, 1066, 1602, 1709
Cac8I GCNNGC 2 cut(s) 96, 1499
CciI TCATGA 1 cut(s) 1138
CfoI GCGC 1 cut(s) 1677
Cfr13I GGNCC 2 cut(s) 451, 1583
CseI GACGC 2 cut(s) 382, 860
Csp6I GTAC 5 cut(s) 1042, 1144, 1153, 1241, 1251
CviAII CATG 5 cut(s) 51, 361, 958, 1139, 1274
CviQI GTAC 5 cut(s) 1042, 1144, 1153, 1241, 1251
DdeI CTNAG 3 cut(s) 571, 1282, 1625
EaeI YGGCCR 1 cut(s) 92
Eam1104I CTCTTC 1 cut(s) 1608
EarI CTCTTC 1 cut(s) 1608
Eco130I CCWWGG 3 cut(s) 360, 1072, 1227
Eco24I GRGCYC 1 cut(s) 1611
Eco47I GGWCC 1 cut(s) 1583
Eco57I CTGAAG 1 cut(s) 198
Eco81I CCTNAGG 2 cut(s) 1282, 1625
EcoO109I RGGNCCY 1 cut(s) 451
EcoRII CCWGG 1 cut(s) 281
EcoT14I CCWWGG 3 cut(s) 360, 1072, 1227
EcoT38I GRGCYC 1 cut(s) 1611
ErhI CCWWGG 3 cut(s) 360, 1072, 1227
FaeI CATG 5 cut(s) 54, 364, 961, 1142, 1277
FaqI GGGAC 1 cut(s) 24
FatI CATG 5 cut(s) 50, 360, 957, 1138, 1273
FauI CCCGC 1 cut(s) 1285
Fnu4HI GCNGC 8 cut(s) 60, 297, 426, 1098, 1426, 1655, 1673, 1719
FokI GGATG 4 cut(s) 264, 1142, 1325, 1808
FriOI GRGCYC 1 cut(s) 1611
Fsp4HI GCNGC 8 cut(s) 60, 297, 426, 1098, 1426, 1655, 1673, 1719
FspBI CTAG 4 cut(s) 47, 731, 1038, 1166
FspI TGCGCA 1 cut(s) 1676
GlaI GCGC 1 cut(s) 1676
GluI GCNGC 8 cut(s) 60, 297, 426, 1098, 1426, 1655, 1673, 1719
GsaI CCCAGC 1 cut(s) 665
GsuI CTGGAG 2 cut(s) 742, 934
HaeIII GGCC 3 cut(s) 94, 453, 1211
HapII CCGG 1 cut(s) 528
HgaI GACGC 2 cut(s) 382, 860
HhaI GCGC 1 cut(s) 1677
Hin1I GRCGYC 1 cut(s) 393
Hin1II CATG 5 cut(s) 54, 364, 961, 1142, 1277
Hin6I GCGC 1 cut(s) 1675
HinP1I GCGC 1 cut(s) 1675
HincII GTYRAC 1 cut(s) 1420
HindII GTYRAC 1 cut(s) 1420
HindIII AAGCTT 1 cut(s) 1499
HinfI GANTC 7 cut(s) 826, 856, 886, 1122, 1379, 1389, 1799
HpaII CCGG 1 cut(s) 528
Hpy166II GTNNAC 2 cut(s) 728, 1420
Hpy188I TCNGA 6 cut(s) 328, 594, 1398, 1516, 1561, 1787
Hpy8I GTNNAC 2 cut(s) 728, 1420
Hpy99I CGWCG 1 cut(s) 38
HpyAV CCTTC 2 cut(s) 625, 1403
HpyCH4III ACNGT 3 cut(s) 381, 590, 1634
HpyCH4IV ACGT 5 cut(s) 541, 751, 1134, 1239, 1829
HpyF10VI GCNNNNNNNGC 3 cut(s) 56, 431, 581
HpyF3I CTNAG 3 cut(s) 571, 1282, 1625
HpySE526I ACGT 5 cut(s) 541, 751, 1134, 1239, 1829
Hsp92I GRCGYC 1 cut(s) 393
Hsp92II CATG 5 cut(s) 54, 364, 961, 1142, 1277
HspAI GCGC 1 cut(s) 1675
Kpn2I TCCGGA 1 cut(s) 527
LmnI GCTCC 6 cut(s) 56, 273, 609, 707, 739, 915
Lsp1109I GCAGC 6 cut(s) 46, 308, 412, 1412, 1659, 1705
LweI GCATC 2 cut(s) 1303, 1684
MaeI CTAG 4 cut(s) 47, 731, 1038, 1166
MaeII ACGT 5 cut(s) 541, 751, 1134, 1239, 1829
MaeIII GTNAC 4 cut(s) 63, 301, 371, 667
MboII GAAGA 6 cut(s) 224, 227, 248, 257, 388, 1625
MfeI CAATTG 1 cut(s) 1803
MflI RGATCY 3 cut(s) 530, 1342, 1834
MhlI GDGCHC 5 cut(s) 572, 614, 1165, 1611, 1709
MlyI GAGTC 1 cut(s) 1808
MmeI TCCRAC 4 cut(s) 958, 1320, 1366, 1812
MroI TCCGGA 1 cut(s) 527
MseI TTAA 2 cut(s) 20, 1572
MslI CAYNNNNRTG 1 cut(s) 359
MspA1I CMGCKG 3 cut(s) 299, 425, 665
MspI CCGG 1 cut(s) 528
MspR9I CCNGG 1 cut(s) 283
MunI CAATTG 1 cut(s) 1803
Mva1269I GAATGC 1 cut(s) 1169
MvaI CCWGG 1 cut(s) 283
MvnI CGCG 3 cut(s) 854, 1100, 1657
MwoI GCNNNNNNNGC 3 cut(s) 56, 431, 581
NcoI CCATGG 1 cut(s) 360
NlaIII CATG 5 cut(s) 54, 364, 961, 1142, 1277
NlaIV GGNNCC 8 cut(s) 275, 532, 917, 1374, 1400, 1537, 1584, 1780
NmeAIII GCCGAG 1 cut(s) 208
NmuCI GTSAC 1 cut(s) 667
NsbI TGCGCA 1 cut(s) 1676
NspI RCATGY 1 cut(s) 54
PagI TCATGA 1 cut(s) 1138
PcsI WCGNNNNNNNCGW 1 cut(s) 748
PctI GAATGC 1 cut(s) 1169
PfeI GAWTC 6 cut(s) 826, 856, 886, 1122, 1379, 1389
PkrI GCNGC 8 cut(s) 61, 298, 427, 1099, 1427, 1656, 1674, 1720
PleI GAGTC 1 cut(s) 1807
PpsI GAGTC 1 cut(s) 1807
PsiI TTATAA 1 cut(s) 317
Psp1406I AACGTT 2 cut(s) 541, 1829
Psp6I CCWGG 1 cut(s) 281
PspFI CCCAGC 1 cut(s) 661
PspGI CCWGG 1 cut(s) 281
PspN4I GGNNCC 8 cut(s) 275, 532, 917, 1374, 1400, 1537, 1584, 1780
PspPI GGNCC 2 cut(s) 451, 1583
PstI CTGCAG 1 cut(s) 430
PsuI RGATCY 3 cut(s) 530, 1342, 1834
PvuII CAGCTG 3 cut(s) 299, 425, 665
RsaI GTAC 5 cut(s) 1043, 1145, 1154, 1242, 1252
RsaNI GTAC 5 cut(s) 1042, 1144, 1153, 1241, 1251
RseI CAYNNNNRTG 1 cut(s) 359
SaqAI TTAA 2 cut(s) 20, 1572
SatI GCNGC 8 cut(s) 60, 297, 426, 1098, 1426, 1655, 1673, 1719
Sau96I GGNCC 2 cut(s) 451, 1583
ScaI AGTACT 2 cut(s) 1145, 1154
SchI GAGTC 1 cut(s) 1808
ScrFI CCNGG 1 cut(s) 283
SduI GDGCHC 5 cut(s) 572, 614, 1165, 1611, 1709
SfaNI GCATC 2 cut(s) 1303, 1684
SfcI CTRYAG 2 cut(s) 426, 1156
SinI GGWCC 1 cut(s) 1583
SmiMI CAYNNNNRTG 1 cut(s) 359
SmlI CTYRAG 4 cut(s) 116, 1502, 1540, 1753
SmoI CTYRAG 4 cut(s) 116, 1502, 1540, 1753
SsiI CCGC 7 cut(s) 777, 998, 1098, 1248, 1292, 1655, 1776
SspI AATATT 1 cut(s) 128
SspMI CTAG 4 cut(s) 47, 731, 1038, 1166
StyD4I CCNGG 1 cut(s) 281
StyI CCWWGG 3 cut(s) 360, 1072, 1227
TaaI ACNGT 3 cut(s) 381, 590, 1634
TaiI ACGT 5 cut(s) 544, 754, 1137, 1242, 1832
TaqI TCGA 6 cut(s) 33, 350, 597, 1008, 1256, 1611
TaqII GACCGA 1 cut(s) 732
TatI WGTACW 2 cut(s) 1143, 1152
TauI GCSGC 2 cut(s) 1100, 1657
TfiI GAWTC 6 cut(s) 826, 856, 886, 1122, 1379, 1389
Tru1I TTAA 2 cut(s) 20, 1572
Tru9I TTAA 2 cut(s) 20, 1572
TscAI CASTG 4 cut(s) 384, 1066, 1609, 1709
TseFI GTSAC 1 cut(s) 667
TseI GCWGC 6 cut(s) 59, 296, 425, 1425, 1672, 1718
Tsp45I GTSAC 1 cut(s) 667
TspDTI ATGAA 7 cut(s) 568, 818, 919, 1127, 1162, 1365, 1875
TspGWI ACGGA 2 cut(s) 1372, 1575
TspRI CASTG 4 cut(s) 384, 1066, 1609, 1709
VpaK11BI GGWCC 1 cut(s) 1583
XapI RAATTY 3 cut(s) 1508, 1545, 1731
XceI RCATGY 1 cut(s) 54
XcmI CCANNNNNNNNNTGG 1 cut(s) 1789
XspI CTAG 4 cut(s) 47, 731, 1038, 1166
ZrmI AGTACT 2 cut(s) 1145, 1154
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.