FvH4_5g35251

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000708
Physical Location & Seq
Forward (+)
25806337 .. 25811783
5447 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g35251.t1

Sequence Viewer

Length: 630 bp
ATGGAAAACCGTGACCTGCGAGGTTCTCACTCGCCGATCGACGTCTTCAAGTACCGCCATCCTCAACCGTCGCCGATCGCGCCGCAGACAGCAGAGGTTGACGGAAGCTACGGCTCCAAAGGCCAGCTTGTACATGGAAAAGAAAATCTGCATCCAACTTTCCCCGCTAATAACAAATCTGAGCACAGTTGTGCAAGTCTACAAGCAGCTCAGGATTATACACTCAGCCCAAAGTCCCACTTTGCCCTTCACGTCATGGCATCAGCTGTTACAAAAGCAACAGCATTGACCTCATCGCCAAAGCCAAGTAAGACCAGCGAGACCCCAAATAATGTCATCAGCTGCAAGGACTGGTCACCCGAAGATGATGGCATTACGGTTATCTTGTCTCCAACCGAAATACCTTCATCGAAGGAAGACATGGATGCATCCAGCACTGCTTTGCCGAGAGGCGGCGACGTCGAGGTGAAGGAGTTGGACATAGTGGGGGTTGGGTTTGGGTACGATTCAGAGAGGGAAGAAGCCGGATCTGGTGGAGAGAAAGAGAGTGAGGGTTGGGTGATGGTGAGGGAAAAAAAGAGAAGAGGTGGTGGAGGGTTGGGTTTGATTGATCTGAGGAAGAAGAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04218 ko05166 ko05167 map04218 map05166 map05167
Pfam Domains
Protein Families
zf-CCCH

Protein Analysis

210

Amino Acids

22.46

Weight (kDa)

5.82

Isoelectric Point (pI)

55.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 2 cut(s) 45, 462
Acc36I ACCTGC 1 cut(s) 24
AccI GTMKAC 1 cut(s) 199
AccII CGCG 1 cut(s) 80
AciI CCGC 4 cut(s) 55, 83, 165, 453
AclWI GGATC 1 cut(s) 535
AcyI GRCGYC 2 cut(s) 42, 459
AfaI GTAC 3 cut(s) 53, 132, 503
AfiI CCNNNNNNNGG 1 cut(s) 452
AgsI TTSAA 1 cut(s) 49
AjiI CACGTC 1 cut(s) 253
AleI CACNNNNGTG 1 cut(s) 189
AluBI AGCT 5 cut(s) 108, 127, 209, 266, 342
AluI AGCT 5 cut(s) 108, 127, 209, 266, 342
Alw21I GWGCWC 1 cut(s) 186
Alw26I GTCTC 2 cut(s) 314, 393
AlwI GGATC 1 cut(s) 535
AoxI GGCC 1 cut(s) 121
ApeKI GCWGC 2 cut(s) 206, 342
AspLEI GCGC 1 cut(s) 82
AsuHPI GGTGA 4 cut(s) 348, 478, 571, 577
BbsI GAAGAC 2 cut(s) 37, 423
Bbv12I GWGCWC 1 cut(s) 186
BbvI GCAGC 2 cut(s) 218, 329
BccI CCATC 3 cut(s) 66, 362, 556
BceAI ACGGC 1 cut(s) 127
BcoDI GTCTC 2 cut(s) 314, 393
BfuAI ACCTGC 1 cut(s) 24
BisI GCNGC 4 cut(s) 83, 207, 343, 454
BlsI GCNGC 4 cut(s) 84, 208, 344, 455
BmgBI CACGTC 1 cut(s) 253
BmiI GGNNCC 1 cut(s) 115
BmsI GCATC 4 cut(s) 160, 269, 415, 437
BpiI GAAGAC 2 cut(s) 37, 423
Bpu10I CCTNAGC 1 cut(s) 210
BsaHI GRCGYC 2 cut(s) 42, 459
BsaI GGTCTC 1 cut(s) 314
Bsc4I CCNNNNNNNGG 1 cut(s) 452
Bse1I ACTGG 1 cut(s) 356
BseGI GGATG 4 cut(s) 58, 151, 428, 430
BseLI CCNNNNNNNGG 1 cut(s) 452
BseMII CTCAG 4 cut(s) 171, 224, 238, 605
BseNI ACTGG 1 cut(s) 356
BseXI GCAGC 2 cut(s) 218, 329
Bsh1236I CGCG 1 cut(s) 80
Bsh1285I CGRYCG 2 cut(s) 39, 78
BshFI GGCC 1 cut(s) 123
BsiEI CGRYCG 2 cut(s) 39, 78
BsiHKAI GWGCWC 1 cut(s) 186
BsiSI CCGG 1 cut(s) 525
BslFI GGGAC 1 cut(s) 220
BslI CCNNNNNNNGG 1 cut(s) 452
BsmAI GTCTC 2 cut(s) 314, 393
BsmFI GGGAC 1 cut(s) 220
BsnI GGCC 1 cut(s) 123
Bso31I GGTCTC 1 cut(s) 314
Bsp1286I GDGCHC 1 cut(s) 186
Bsp1407I TGTACA 1 cut(s) 130
Bsp143I GATC 4 cut(s) 36, 75, 527, 610
BspACI CCGC 4 cut(s) 55, 83, 165, 453
BspANI GGCC 1 cut(s) 123
BspCNI CTCAG 4 cut(s) 172, 223, 237, 606
BspFNI CGCG 1 cut(s) 80
BspLI GGNNCC 1 cut(s) 115
BspMI ACCTGC 1 cut(s) 24
BspPI GGATC 1 cut(s) 535
BspTNI GGTCTC 1 cut(s) 314
BsrGI TGTACA 1 cut(s) 130
BsrI ACTGG 1 cut(s) 356
BssMI GATC 4 cut(s) 36, 75, 527, 610
BssNI GRCGYC 2 cut(s) 42, 459
Bst4CI ACNGT 4 cut(s) 11, 69, 188, 379
Bst6I CTCTTC 1 cut(s) 577
BstACI GRCGYC 2 cut(s) 42, 459
BstAUI TGTACA 1 cut(s) 130
BstC8I GCNNGC 1 cut(s) 125
BstDEI CTNAG 4 cut(s) 180, 210, 224, 614
BstEII GGTNACC 1 cut(s) 354
BstF5I GGATG 4 cut(s) 58, 151, 428, 430
BstFNI CGCG 1 cut(s) 80
BstHHI GCGC 1 cut(s) 82
BstKTI GATC 4 cut(s) 39, 78, 530, 613
BstMAI GTCTC 2 cut(s) 314, 393
BstMBI GATC 4 cut(s) 36, 75, 527, 610
BstMCI CGRYCG 2 cut(s) 39, 78
BstMWI GCNNNNNNNGC 2 cut(s) 79, 120
BstPI GGTNACC 1 cut(s) 354
BstUI CGCG 1 cut(s) 80
BstV1I GCAGC 2 cut(s) 218, 329
BstV2I GAAGAC 2 cut(s) 37, 423
BstX2I RGATCY 1 cut(s) 527
BstYI RGATCY 1 cut(s) 527
BsuRI GGCC 1 cut(s) 123
BtgZI GCGATG 1 cut(s) 279
BtrI CACGTC 1 cut(s) 253
BtsCI GGATG 4 cut(s) 58, 151, 428, 430
BtsI GCAGTG 1 cut(s) 435
BtsIMutI CAGTG 1 cut(s) 435
BveI ACCTGC 1 cut(s) 24
Cac8I GCNNGC 1 cut(s) 125
CfoI GCGC 1 cut(s) 82
Csp6I GTAC 3 cut(s) 52, 131, 502
CviAII CATG 3 cut(s) 134, 256, 421
CviQI GTAC 3 cut(s) 52, 131, 502
DdeI CTNAG 4 cut(s) 180, 210, 224, 614
DpnI GATC 4 cut(s) 38, 77, 529, 612
DpnII GATC 4 cut(s) 36, 75, 527, 610
Eam1104I CTCTTC 1 cut(s) 577
EarI CTCTTC 1 cut(s) 577
Eco31I GGTCTC 1 cut(s) 314
Eco91I GGTNACC 1 cut(s) 354
EcoO65I GGTNACC 1 cut(s) 354
EcoT22I ATGCAT 1 cut(s) 430
FaeI CATG 3 cut(s) 137, 259, 424
FaiI YATR 5 cut(s) 135, 219, 257, 422, 482
FalI AAGNNNNNCTT 4 cut(s) 111, 143, 224, 256
FaqI GGGAC 1 cut(s) 220
FatI CATG 3 cut(s) 133, 255, 420
FauI CCCGC 1 cut(s) 172
FblI GTMKAC 1 cut(s) 199
Fnu4HI GCNGC 4 cut(s) 83, 207, 343, 454
FokI GGATG 4 cut(s) 45, 138, 415, 437
Fsp4HI GCNGC 4 cut(s) 83, 207, 343, 454
GlaI GCGC 1 cut(s) 81
GluI GCNGC 4 cut(s) 83, 207, 343, 454
HaeIII GGCC 1 cut(s) 123
HapII CCGG 1 cut(s) 525
HhaI GCGC 1 cut(s) 82
Hin1I GRCGYC 2 cut(s) 42, 459
Hin1II CATG 3 cut(s) 137, 259, 424
Hin6I GCGC 1 cut(s) 80
HinP1I GCGC 1 cut(s) 80
HincII GTYRAC 1 cut(s) 100
HindII GTYRAC 1 cut(s) 100
HinfI GANTC 1 cut(s) 506
HpaII CCGG 1 cut(s) 525
HphI GGTGA 4 cut(s) 348, 478, 571, 577
Hpy166II GTNNAC 2 cut(s) 100, 200
Hpy188I TCNGA 3 cut(s) 181, 511, 615
Hpy188III TCNNGA 1 cut(s) 212
Hpy8I GTNNAC 2 cut(s) 100, 200
Hpy99I CGWCG 4 cut(s) 44, 73, 461, 464
HpyAV CCTTC 4 cut(s) 257, 406, 414, 463
HpyCH4III ACNGT 4 cut(s) 11, 69, 188, 379
HpyCH4IV ACGT 3 cut(s) 42, 252, 459
HpyCH4V TGCA 4 cut(s) 151, 194, 345, 428
HpyF10VI GCNNNNNNNGC 2 cut(s) 79, 120
HpyF3I CTNAG 4 cut(s) 180, 210, 224, 614
HpySE526I ACGT 3 cut(s) 42, 252, 459
Hsp92I GRCGYC 2 cut(s) 42, 459
Hsp92II CATG 3 cut(s) 137, 259, 424
HspAI GCGC 1 cut(s) 80
Kzo9I GATC 4 cut(s) 36, 75, 527, 610
LmnI GCTCC 1 cut(s) 119
LpnPI CCDG 8 cut(s) 29, 137, 197, 328, 337, 445, 516, 538
Lsp1109I GCAGC 2 cut(s) 218, 329
LweI GCATC 4 cut(s) 160, 269, 415, 437
MaeII ACGT 3 cut(s) 42, 252, 459
MaeIII GTNAC 3 cut(s) 11, 268, 354
MalI GATC 4 cut(s) 38, 77, 529, 612
MboI GATC 4 cut(s) 36, 75, 527, 610
MboII GAAGA 5 cut(s) 37, 374, 428, 530, 594
MflI RGATCY 1 cut(s) 527
MhlI GDGCHC 1 cut(s) 186
MluCI AATT 1 cut(s) 625
MmeI TCCRAC 3 cut(s) 179, 416, 456
Mph1103I ATGCAT 1 cut(s) 430
MslI CAYNNNNRTG 1 cut(s) 189
MspA1I CMGCKG 2 cut(s) 266, 342
MspI CCGG 1 cut(s) 525
MvnI CGCG 1 cut(s) 80
MwoI GCNNNNNNNGC 2 cut(s) 79, 120
NdeII GATC 4 cut(s) 36, 75, 527, 610
NlaIII CATG 3 cut(s) 137, 259, 424
NlaIV GGNNCC 1 cut(s) 115
NmeAIII GCCGAG 1 cut(s) 471
NmuCI GTSAC 2 cut(s) 11, 354
NsiI ATGCAT 1 cut(s) 430
OliI CACNNNNGTG 1 cut(s) 189
PfeI GAWTC 1 cut(s) 506
PkrI GCNGC 4 cut(s) 84, 208, 344, 455
Ple19I CGATCG 2 cut(s) 39, 78
PspEI GGTNACC 1 cut(s) 354
PspN4I GGNNCC 1 cut(s) 115
PsuI RGATCY 1 cut(s) 527
PvuI CGATCG 2 cut(s) 39, 78
PvuII CAGCTG 2 cut(s) 266, 342
RsaI GTAC 3 cut(s) 53, 132, 503
RsaNI GTAC 3 cut(s) 52, 131, 502
RseI CAYNNNNRTG 1 cut(s) 189
SatI GCNGC 4 cut(s) 83, 207, 343, 454
Sau3AI GATC 4 cut(s) 36, 75, 527, 610
SduI GDGCHC 1 cut(s) 186
SfaNI GCATC 4 cut(s) 160, 269, 415, 437
SmiMI CAYNNNNRTG 1 cut(s) 189
Sse9I AATT 1 cut(s) 625
SsiI CCGC 4 cut(s) 55, 83, 165, 453
TaaI ACNGT 4 cut(s) 11, 69, 188, 379
TaiI ACGT 3 cut(s) 45, 255, 462
TaqI TCGA 3 cut(s) 39, 410, 462
TasI AATT 1 cut(s) 625
TatI WGTACW 1 cut(s) 130
TauI GCSGC 2 cut(s) 85, 456
TfiI GAWTC 1 cut(s) 506
TscAI CASTG 1 cut(s) 442
TseFI GTSAC 2 cut(s) 11, 354
TseI GCWGC 2 cut(s) 206, 342
Tsp45I GTSAC 2 cut(s) 11, 354
TspDTI ATGAA 1 cut(s) 396
TspGWI ACGGA 1 cut(s) 117
TspRI CASTG 1 cut(s) 442
XcmI CCANNNNNNNNNTGG 1 cut(s) 131
XmiI GTMKAC 1 cut(s) 199
ZraI GACGTC 2 cut(s) 43, 460
Zsp2I ATGCAT 1 cut(s) 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.