MD08G1222100.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0000708
Physical Location & Seq
Forward (+)
28474803 .. 28477794
2992 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1222100.v1.1.491

Sequence Viewer

Length: 864 bp
ATGGCGAAATTGGACACACGATTGTTCAGAGGGAACTTGGAGAATGTGGACGTCAACACGCCGTTGTTCAGGTACTTGTGCGCTCAGTCTCACTCTCCGTCACTTTCTCCGACGGACACCTTGAAGCAACGTCGCCGCCACCACTCGCCGCTGTCGCCTCTGGAGAATCTGATGCAGAGGTCGCCGTTCTCGCCTCTGGAGAATCTGATGCAAAGGTCGCCGCACTCTCCTCTGCAGAATCTGATGCAGAGGCCGCCGCCTCCGACAGTTTACAGAACGCCGATAGGAGCGGTGGAGAGGGAGGAGGTTTTGGTGATGGACGGTGTTTTGGTCACCGGAGGAGGAGGGAGGAGTTCGAGGTCCGCATCATCATCGGATTATTTGGGGAAGACGCCGTGCAAGACTGATCTTTACAGGTCCTGGGAGGATTCTCGAAGTTACGCTTCCAAATACCAGTGTGCTTCTGGAAAAGAAAAGCAGCGTCCAACTCGTTGCCCTGTCAAGAACAAACCTGAGGCACATATATGCAAGTCATACACTGCCACAAGTTATCCATGCCCACCCAGCCCCAAGTCCCGATTTATCCATCCCGTCATGCCATCTTCAGTTACAGAAGCAGCAGGAGCCAGAACACAAGCAGCCTCATCGACTACACCCAAAAACGCCATATCAATCCCTGCTACCACCACCATCTGCCTGGACTGGTCACCCCAAGATGATGGCATTGAGGTTGTATTGCCGCATTCATCAACTGGAACACCTCCATCAAGGGAAGATATAGATTCCTACATCAGCGACGTTCTTTGTGGTCCCACTAAAGGAAGGAGATTACGAGTGTTTGCTGAGATTTATGCAGTGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04218 ko05166 ko05167 map04218 map05166 map05167
Pfam Domains
Protein Families
zf-CCCH

Protein Analysis

288

Amino Acids

31.67

Weight (kDa)

9.28

Isoelectric Point (pI)

71.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 54
AccBSI CCGCTC 1 cut(s) 290
AciI CCGC 8 cut(s) 136, 149, 221, 254, 257, 290, 363, 740
AcuI CTGAAG 1 cut(s) 588
AcyI GRCGYC 2 cut(s) 51, 392
AfaI GTAC 1 cut(s) 74
AfiI CCNNNNNNNGG 1 cut(s) 818
AgsI TTSAA 1 cut(s) 124
AjnI CCWGG 2 cut(s) 419, 696
Alw26I GTCTC 1 cut(s) 93
AlwNI CAGNNNCTG 2 cut(s) 241, 420
AoxI GGCC 1 cut(s) 251
ApeKI GCWGC 3 cut(s) 478, 617, 638
AspLEI GCGC 1 cut(s) 83
AspS9I GGNCC 3 cut(s) 360, 417, 809
AsuHPI GGTGA 3 cut(s) 325, 325, 699
AvaII GGWCC 3 cut(s) 360, 417, 809
AxyI CCTNAGG 1 cut(s) 513
BbsI GAAGAC 1 cut(s) 395
BbvI GCAGC 3 cut(s) 490, 629, 650
BccI CCATC 6 cut(s) 310, 594, 607, 698, 713, 772
BceAI ACGGC 3 cut(s) 46, 169, 379
BcgI CGANNNNNNTGC 4 cut(s) 354, 388, 627, 661
BciT130I CCWGG 2 cut(s) 421, 698
BcoDI GTCTC 1 cut(s) 93
BfmI CTRYAG 1 cut(s) 233
BisI GCNGC 9 cut(s) 136, 149, 221, 254, 257, 479, 618, 639, 740
BlsI GCNGC 9 cut(s) 137, 150, 222, 255, 258, 480, 619, 640, 741
Bme1390I CCNGG 2 cut(s) 421, 698
Bme18I GGWCC 3 cut(s) 360, 417, 809
BmgT120I GGNCC 3 cut(s) 360, 417, 809
BmiI GGNNCC 2 cut(s) 625, 811
BmrFI CCNGG 2 cut(s) 421, 698
BmsI GCATC 4 cut(s) 162, 198, 234, 374
BpiI GAAGAC 1 cut(s) 395
BpmI CTGGAG 2 cut(s) 182, 218
BsaHI GRCGYC 2 cut(s) 51, 392
BsaJI CCNNGG 1 cut(s) 420
BsaWI WCCGGW 1 cut(s) 335
Bsc4I CCNNNNNNNGG 1 cut(s) 818
Bse1I ACTGG 3 cut(s) 454, 707, 757
Bse21I CCTNAGG 1 cut(s) 513
BseBI CCWGG 2 cut(s) 421, 698
BseDI CCNNGG 1 cut(s) 420
BseGI GGATG 1 cut(s) 586
BseLI CCNNNNNNNGG 1 cut(s) 818
BseMII CTCAG 3 cut(s) 98, 504, 834
BseNI ACTGG 3 cut(s) 454, 707, 757
BseRI GAGGAG 5 cut(s) 219, 317, 354, 357, 364
BseXI GCAGC 3 cut(s) 490, 629, 650
BseYI CCCAGC 1 cut(s) 563
BshFI GGCC 1 cut(s) 253
BsiSI CCGG 1 cut(s) 336
BslFI GGGAC 2 cut(s) 559, 795
BslI CCNNNNNNNGG 1 cut(s) 818
BsmAI GTCTC 1 cut(s) 93
BsmFI GGGAC 2 cut(s) 559, 795
BsmI GAATGC 1 cut(s) 742
BsnI GGCC 1 cut(s) 253
Bsp143I GATC 1 cut(s) 406
BspACI CCGC 8 cut(s) 136, 149, 221, 254, 257, 290, 363, 740
BspANI GGCC 1 cut(s) 253
BspCNI CTCAG 3 cut(s) 97, 505, 835
BspLI GGNNCC 2 cut(s) 625, 811
BspMAI CTGCAG 1 cut(s) 237
BsrBI CCGCTC 1 cut(s) 290
BsrI ACTGG 3 cut(s) 454, 707, 757
BssECI CCNNGG 1 cut(s) 420
BssMI GATC 1 cut(s) 406
BssNI GRCGYC 2 cut(s) 51, 392
Bst2UI CCWGG 2 cut(s) 421, 698
Bst4CI ACNGT 2 cut(s) 268, 323
BstACI GRCGYC 2 cut(s) 51, 392
BstDEI CTNAG 3 cut(s) 84, 513, 843
BstEII GGTNACC 2 cut(s) 331, 705
BstF5I GGATG 1 cut(s) 586
BstHHI GCGC 1 cut(s) 83
BstKTI GATC 1 cut(s) 409
BstMAI GTCTC 1 cut(s) 93
BstMBI GATC 1 cut(s) 406
BstMWI GCNNNNNNNGC 7 cut(s) 154, 181, 190, 217, 253, 564, 623
BstNI CCWGG 2 cut(s) 421, 698
BstPI GGTNACC 2 cut(s) 331, 705
BstSCI CCNGG 2 cut(s) 419, 696
BstSFI CTRYAG 1 cut(s) 233
BstV1I GCAGC 3 cut(s) 490, 629, 650
BstV2I GAAGAC 1 cut(s) 395
BstXI CCANNNNNNTGG 2 cut(s) 697, 719
Bsu36I CCTNAGG 1 cut(s) 513
BsuRI GGCC 1 cut(s) 253
BtsCI GGATG 1 cut(s) 586
BtsI GCAGTG 2 cut(s) 537, 861
BtsIMutI CAGTG 3 cut(s) 461, 537, 861
CaiI CAGNNNCTG 2 cut(s) 241, 420
CfoI GCGC 1 cut(s) 83
Cfr13I GGNCC 3 cut(s) 360, 417, 809
CseI GACGC 2 cut(s) 400, 470
Csp6I GTAC 1 cut(s) 73
CviAII CATG 2 cut(s) 555, 595
CviJI RGCY 4 cut(s) 253, 567, 626, 641
CviKI_1 RGCY 4 cut(s) 253, 567, 626, 641
CviQI GTAC 1 cut(s) 73
DdeI CTNAG 3 cut(s) 84, 513, 843
DpnI GATC 1 cut(s) 408
DpnII GATC 1 cut(s) 406
Eco47I GGWCC 3 cut(s) 360, 417, 809
Eco57I CTGAAG 1 cut(s) 588
Eco81I CCTNAGG 1 cut(s) 513
Eco91I GGTNACC 2 cut(s) 331, 705
EcoO109I RGGNCCY 1 cut(s) 417
EcoO65I GGTNACC 2 cut(s) 331, 705
EcoRII CCWGG 2 cut(s) 419, 696
FaeI CATG 2 cut(s) 558, 598
FaiI YATR 9 cut(s) 522, 524, 526, 535, 556, 596, 668, 779, 852
FalI AAGNNNNNCTT 2 cut(s) 427, 459
FaqI GGGAC 2 cut(s) 559, 795
FatI CATG 2 cut(s) 554, 594
Fnu4HI GCNGC 9 cut(s) 136, 149, 221, 254, 257, 479, 618, 639, 740
FokI GGATG 1 cut(s) 573
Fsp4HI GCNGC 9 cut(s) 136, 149, 221, 254, 257, 479, 618, 639, 740
GlaI GCGC 1 cut(s) 82
GluI GCNGC 9 cut(s) 136, 149, 221, 254, 257, 479, 618, 639, 740
GsaI CCCAGC 1 cut(s) 567
GsuI CTGGAG 2 cut(s) 182, 218
HaeIII GGCC 1 cut(s) 253
HapII CCGG 1 cut(s) 336
HgaI GACGC 2 cut(s) 400, 470
HhaI GCGC 1 cut(s) 83
Hin1I GRCGYC 2 cut(s) 51, 392
Hin1II CATG 2 cut(s) 558, 598
Hin6I GCGC 1 cut(s) 81
HinP1I GCGC 1 cut(s) 81
HincII GTYRAC 1 cut(s) 55
HindII GTYRAC 1 cut(s) 55
HinfI GANTC 5 cut(s) 166, 202, 238, 428, 782
HpaII CCGG 1 cut(s) 336
HphI GGTGA 3 cut(s) 325, 325, 699
Hpy166II GTNNAC 3 cut(s) 49, 55, 271
Hpy188I TCNGA 7 cut(s) 29, 111, 171, 207, 243, 264, 376
Hpy188III TCNNGA 6 cut(s) 161, 197, 432, 465, 502, 576
Hpy8I GTNNAC 3 cut(s) 49, 55, 271
Hpy99I CGWCG 3 cut(s) 115, 135, 800
HpyAV CCTTC 1 cut(s) 816
HpyCH4III ACNGT 2 cut(s) 268, 323
HpyCH4IV ACGT 3 cut(s) 51, 130, 798
HpyCH4V TGCA 8 cut(s) 175, 211, 235, 247, 399, 528, 854, 859
HpyF10VI GCNNNNNNNGC 7 cut(s) 154, 181, 190, 217, 253, 564, 623
HpyF3I CTNAG 3 cut(s) 84, 513, 843
HpySE526I ACGT 3 cut(s) 51, 130, 798
Hsp92I GRCGYC 2 cut(s) 51, 392
Hsp92II CATG 2 cut(s) 558, 598
HspAI GCGC 1 cut(s) 81
Kzo9I GATC 1 cut(s) 406
LmnI GCTCC 2 cut(s) 287, 623
Lsp1109I GCAGC 3 cut(s) 490, 629, 650
LweI GCATC 4 cut(s) 162, 198, 234, 374
MaeII ACGT 3 cut(s) 51, 130, 798
MaeIII GTNAC 5 cut(s) 99, 331, 437, 607, 705
MalI GATC 1 cut(s) 408
MbiI CCGCTC 1 cut(s) 290
MboI GATC 1 cut(s) 406
MboII GAAGA 3 cut(s) 400, 594, 785
MluCI AATT 1 cut(s) 8
MmeI TCCRAC 3 cut(s) 134, 287, 509
MslI CAYNNNNRTG 1 cut(s) 523
MspA1I CMGCKG 1 cut(s) 151
MspI CCGG 1 cut(s) 336
MspR9I CCNGG 2 cut(s) 421, 698
Mva1269I GAATGC 1 cut(s) 742
MvaI CCWGG 2 cut(s) 421, 698
MwoI GCNNNNNNNGC 7 cut(s) 154, 181, 190, 217, 253, 564, 623
NdeII GATC 1 cut(s) 406
NlaIII CATG 2 cut(s) 558, 598
NlaIV GGNNCC 2 cut(s) 625, 811
NmuCI GTSAC 3 cut(s) 99, 331, 705
PctI GAATGC 1 cut(s) 742
PfeI GAWTC 5 cut(s) 166, 202, 238, 428, 782
PkrI GCNGC 9 cut(s) 137, 150, 222, 255, 258, 480, 619, 640, 741
PpuMI RGGWCCY 1 cut(s) 417
Psp5II RGGWCCY 1 cut(s) 417
Psp6I CCWGG 2 cut(s) 419, 696
PspEI GGTNACC 2 cut(s) 331, 705
PspFI CCCAGC 1 cut(s) 563
PspGI CCWGG 2 cut(s) 419, 696
PspN4I GGNNCC 2 cut(s) 625, 811
PspPI GGNCC 3 cut(s) 360, 417, 809
PspPPI RGGWCCY 1 cut(s) 417
PstI CTGCAG 1 cut(s) 237
PstNI CAGNNNCTG 2 cut(s) 241, 420
RsaI GTAC 1 cut(s) 74
RsaNI GTAC 1 cut(s) 73
RseI CAYNNNNRTG 1 cut(s) 523
SatI GCNGC 9 cut(s) 136, 149, 221, 254, 257, 479, 618, 639, 740
Sau3AI GATC 1 cut(s) 406
Sau96I GGNCC 3 cut(s) 360, 417, 809
ScrFI CCNGG 2 cut(s) 421, 698
SfaNI GCATC 4 cut(s) 162, 198, 234, 374
SfcI CTRYAG 1 cut(s) 233
SinI GGWCC 3 cut(s) 360, 417, 809
SmiMI CAYNNNNRTG 1 cut(s) 523
Sse9I AATT 1 cut(s) 8
SsiI CCGC 8 cut(s) 136, 149, 221, 254, 257, 290, 363, 740
StyD4I CCNGG 2 cut(s) 419, 696
TaaI ACNGT 2 cut(s) 268, 323
TaiI ACGT 3 cut(s) 54, 133, 801
TaqI TCGA 3 cut(s) 356, 433, 647
TasI AATT 1 cut(s) 8
TauI GCSGC 6 cut(s) 138, 151, 223, 256, 259, 742
TfiI GAWTC 5 cut(s) 166, 202, 238, 428, 782
TscAI CASTG 3 cut(s) 461, 544, 861
TseFI GTSAC 3 cut(s) 99, 331, 705
TseI GCWGC 3 cut(s) 478, 617, 638
Tsp45I GTSAC 3 cut(s) 99, 331, 705
TspDTI ATGAA 1 cut(s) 735
TspGWI ACGGA 2 cut(s) 87, 128
TspRI CASTG 3 cut(s) 461, 544, 861
VpaK11BI GGWCC 3 cut(s) 360, 417, 809
XcmI CCANNNNNNNNNTGG 2 cut(s) 461, 694
ZraI GACGTC 1 cut(s) 52
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.