FvH4_6g28960

Ribosomal_S15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0001441
Physical Location & Seq
Reverse (-)
22354940 .. 22358435
3496 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g28960.t1

Sequence Viewer

Length: 1251 bp
ATGATGGCTCTCCGACTGAGAACCAACCACCGCACCCTAACCAACCCATCTCTCACCCACCACCTCTTCTCCACCTCCTCCTCCTCAGATCCATCCGACCCAACCGAACCGAATCCCCAGTCCTCCTCCTCCTCCATCTCCAATTACTTCAGCGATGTCAAAGGCAGCCTCAGGCAACAGTCACATCAAAGCAGACCCACTCCTCGCATCAACCACCCTCCTCCTCCCGCCCGGCCCTCCAATGCCGATTCCCTGTACGAAATCCGCAAGAATCTCTCGGAGTTTCGCATTCGATCCATGGGACCAAACCACATTGAATCCAACAGCTCTCCGCCCCCGCCGGGCTCGGAGCAGATTTCGTTTTCCGAGCTTTTCAAGAGAAAAGTGAGCAGCGACGGCAAGGCGGTGGAGTTCAAGTCGATTCGGGATAGTCTGAAGCACTTAAGACCCAATGCAAATGTGCAGGGTGAGAAGAAGGGTGAGCCATTGTCGATGAGAAGGTTTCAGAGCATACTAAGGTCGAAGCCGAGCGGCCAGGATGCGGTGGGCAGTGGTGGACTGCCGCGTTTGTTTGATAAGGAGAGGATTGGAGAGAAGAGGGAGGATGGGTTGAAGACTGAGTTTATCAAGATTTATAGCGAAGAGGAGTTGGGGGAGAAGCTGAGGAAGTTGAGGCCCGAGGGCGGCGGGGTGTTTTCGTTTGTGGAGTTGAGTGAGAGGTTGGTCAAGTTGAGGAAGATGGATGATATTGAGGCTCGGTCTCGGACTGGGGGAGTCAATCGCAATGACTTGAGGGATTCGTTGGTCACCTTGAATGAGGGGTATAAGACATCTTCGATTAAGAGGCTTCCTATATTAGAATTGGGTCAAACTCCTGATTATATGACGAAACCCCCGAAGGAAGGCTTGGCTGAGAAGTATTTCCATCCAGATAACATGTCAGCTGCAGAAAAACTAAAAATAGAGCTTGACAAAGTTAGAGATGAATTTAAAATGTCAGAGTCAGATTGTGGTTCTTCACGAGTACAAGTGGCACAGCTCACTACAAAGATCAAACACCTTTCTTCAGTTTTACACAAAAAGGATAAGCATTCACTTAAGGGTCTTCAAGCAATGGTGCAGAGGAGGAAGAAGCTATTGAAGTACCTCCGAAGAACTGACTGGGACTCTTACTGCCTTGTTCTCTCCAAACTTGGTCTCCGGGACACACCAGATGTCAAACAAGAGTACAAGAAACATTACAAGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S15

Protein Analysis

417

Amino Acids

47.28

Weight (kDa)

9.81

Isoelectric Point (pI)

62.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S15 PF00312 326 - 401 1.6e-23 Ribosomal protein S15
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 531
AccII CGCG 1 cut(s) 565
AclWI GGATC 2 cut(s) 83, 288
AcoI YGGCCR 1 cut(s) 532
AcsI RAATTY 1 cut(s) 986
AcuI CTGAAG 3 cut(s) 133, 455, 1050
AfaI GTAC 4 cut(s) 257, 1026, 1145, 1229
AfiI CCNNNNNNNGG 4 cut(s) 341, 541, 683, 902
AflII CTTAAG 2 cut(s) 442, 1097
AflIII ACRYGT 1 cut(s) 936
AgsI TTSAA 7 cut(s) 317, 376, 415, 613, 814, 1109, 1141
AjnI CCWGG 1 cut(s) 534
AjuI GAANNNNNNNTTGG 2 cut(s) 890, 922
AluBI AGCT 7 cut(s) 327, 370, 661, 944, 967, 1039, 1135
AluI AGCT 7 cut(s) 327, 370, 661, 944, 967, 1039, 1135
Alw26I GTCTC 2 cut(s) 765, 1202
AlwI GGATC 2 cut(s) 83, 288
Ama87I CYCGRG 1 cut(s) 677
AoxI GGCC 3 cut(s) 233, 532, 674
ApeKI GCWGC 3 cut(s) 165, 390, 944
ApoI RAATTY 1 cut(s) 986
ArsI GACNNNNNNTTYG 2 cut(s) 104, 136
Asp700I GAANNNNTTC 1 cut(s) 920
AspS9I GGNCC 3 cut(s) 234, 302, 675
AsuC2I CCSGG 3 cut(s) 232, 342, 1202
AsuHPI GGTGA 4 cut(s) 46, 479, 491, 799
AvaI CYCGRG 1 cut(s) 677
AvaII GGWCC 1 cut(s) 302
AxyI CCTNAGG 1 cut(s) 170
BanII GRGCYC 1 cut(s) 347
BauI CACGAG 1 cut(s) 1020
BbsI GAAGAC 2 cut(s) 620, 1097
BbvCI CCTCAGC 1 cut(s) 662
BbvI GCAGC 3 cut(s) 177, 402, 931
BccI CCATC 6 cut(s) 55, 100, 143, 599, 733, 933
BceAI ACGGC 1 cut(s) 412
BciT130I CCWGG 1 cut(s) 536
BcnI CCSGG 3 cut(s) 232, 342, 1202
BcoDI GTCTC 2 cut(s) 765, 1202
BfmI CTRYAG 1 cut(s) 945
BfrI CTTAAG 2 cut(s) 442, 1097
BisI GCNGC 6 cut(s) 166, 391, 532, 563, 685, 945
BlsI GCNGC 6 cut(s) 167, 392, 533, 564, 686, 946
Bme1390I CCNGG 4 cut(s) 232, 342, 536, 1202
Bme18I GGWCC 1 cut(s) 302
BmeT110I CYCGRG 1 cut(s) 677
BmgT120I GGNCC 3 cut(s) 234, 302, 675
BmiI GGNNCC 1 cut(s) 303
BmrFI CCNGG 4 cut(s) 232, 342, 536, 1202
BmrI ACTGGG 3 cut(s) 112, 777, 1171
BmsI GCATC 2 cut(s) 216, 529
BmuI ACTGGG 3 cut(s) 112, 777, 1171
BpiI GAAGAC 2 cut(s) 620, 1097
Bpu10I CCTNAGC 1 cut(s) 662
BpuEI CTTGAG 1 cut(s) 811
BpuMI CCSGG 3 cut(s) 232, 342, 1202
BsaI GGTCTC 2 cut(s) 765, 1202
BsaJI CCNNGG 2 cut(s) 297, 678
BsaXI ACNNNNNCTCC 8 cut(s) 53, 83, 401, 431, 593, 623, 1182, 1212
Bsc4I CCNNNNNNNGG 4 cut(s) 341, 541, 683, 902
Bse1I ACTGG 3 cut(s) 118, 772, 1166
Bse21I CCTNAGG 1 cut(s) 170
Bse3DI GCAATG 2 cut(s) 790, 1119
BseBI CCWGG 1 cut(s) 536
BseDI CCNNGG 2 cut(s) 297, 678
BseGI GGATG 5 cut(s) 92, 544, 610, 748, 925
BseLI CCNNNNNNNGG 4 cut(s) 341, 541, 683, 902
BseMI GCAATG 2 cut(s) 790, 1119
BseMII CTCAG 6 cut(s) 8, 99, 184, 609, 653, 903
BseNI ACTGG 3 cut(s) 118, 772, 1166
BseXI GCAGC 3 cut(s) 177, 402, 931
BsgI GTGCAG 2 cut(s) 482, 1139
Bsh1236I CGCG 1 cut(s) 565
BshFI GGCC 3 cut(s) 235, 534, 676
BsiHKCI CYCGRG 1 cut(s) 677
BsiSI CCGG 3 cut(s) 232, 341, 1201
BslFI GGGAC 3 cut(s) 315, 1178, 1217
BslI CCNNNNNNNGG 4 cut(s) 341, 541, 683, 902
BsmAI GTCTC 2 cut(s) 765, 1202
BsmFI GGGAC 3 cut(s) 315, 1178, 1217
BsmI GAATGC 2 cut(s) 288, 1090
BsnI GGCC 3 cut(s) 235, 534, 676
Bso31I GGTCTC 2 cut(s) 765, 1202
BsoBI CYCGRG 1 cut(s) 677
Bsp1286I GDGCHC 1 cut(s) 347
Bsp143I GATC 3 cut(s) 88, 293, 1050
Bsp19I CCATGG 1 cut(s) 297
BspANI GGCC 3 cut(s) 235, 534, 676
BspCNI CTCAG 6 cut(s) 9, 98, 183, 610, 654, 904
BspFNI CGCG 1 cut(s) 565
BspLI GGNNCC 1 cut(s) 303
BspMAI CTGCAG 1 cut(s) 949
BspPI GGATC 2 cut(s) 83, 288
BspTI CTTAAG 2 cut(s) 442, 1097
BspTNI GGTCTC 2 cut(s) 765, 1202
BsrBI CCGCTC 1 cut(s) 531
BsrDI GCAATG 2 cut(s) 790, 1119
BsrI ACTGG 3 cut(s) 118, 772, 1166
BssECI CCNNGG 2 cut(s) 297, 678
BssMI GATC 3 cut(s) 88, 293, 1050
BssSI CACGAG 1 cut(s) 1020
BssT1I CCWWGG 1 cut(s) 297
Bst2BI CACGAG 1 cut(s) 1020
Bst2UI CCWGG 1 cut(s) 536
Bst4CI ACNGT 1 cut(s) 180
Bst6I CTCTTC 3 cut(s) 71, 590, 636
BstAFI CTTAAG 2 cut(s) 442, 1097
BstDEI CTNAG 7 cut(s) 17, 85, 170, 515, 618, 662, 912
BstDSI CCRYGG 1 cut(s) 297
BstEII GGTNACC 1 cut(s) 805
BstF5I GGATG 5 cut(s) 92, 544, 610, 748, 925
BstFNI CGCG 1 cut(s) 565
BstKTI GATC 3 cut(s) 91, 296, 1053
BstMAI GTCTC 2 cut(s) 765, 1202
BstMBI GATC 3 cut(s) 88, 293, 1050
BstMWI GCNNNNNNNGC 1 cut(s) 396
BstNI CCWGG 1 cut(s) 536
BstNSI RCATGY 1 cut(s) 940
BstPI GGTNACC 1 cut(s) 805
BstSCI CCNGG 4 cut(s) 230, 340, 534, 1200
BstSFI CTRYAG 1 cut(s) 945
BstUI CGCG 1 cut(s) 565
BstV1I GCAGC 3 cut(s) 177, 402, 931
BstV2I GAAGAC 2 cut(s) 620, 1097
BstX2I RGATCY 1 cut(s) 88
BstYI RGATCY 1 cut(s) 88
Bsu36I CCTNAGG 1 cut(s) 170
BsuRI GGCC 3 cut(s) 235, 534, 676
BtgI CCRYGG 1 cut(s) 297
BtgZI GCGATG 1 cut(s) 168
BtsCI GGATG 5 cut(s) 92, 544, 610, 748, 925
BtsI GCAGTG 1 cut(s) 556
BtsIMutI CAGTG 1 cut(s) 556
Cfr13I GGNCC 3 cut(s) 234, 302, 675
Csp6I GTAC 4 cut(s) 256, 1025, 1144, 1228
CviAII CATG 2 cut(s) 298, 937
CviQI GTAC 4 cut(s) 256, 1025, 1144, 1228
DdeI CTNAG 7 cut(s) 17, 85, 170, 515, 618, 662, 912
DpnI GATC 3 cut(s) 90, 295, 1052
DpnII GATC 3 cut(s) 88, 293, 1050
DraI TTTAAA 1 cut(s) 991
EaeI YGGCCR 1 cut(s) 532
Eam1104I CTCTTC 3 cut(s) 71, 590, 636
EarI CTCTTC 3 cut(s) 71, 590, 636
EciI GGCGGA 1 cut(s) 321
Eco130I CCWWGG 1 cut(s) 297
Eco24I GRGCYC 1 cut(s) 347
Eco31I GGTCTC 2 cut(s) 765, 1202
Eco47I GGWCC 1 cut(s) 302
Eco57I CTGAAG 3 cut(s) 133, 455, 1050
Eco81I CCTNAGG 1 cut(s) 170
Eco88I CYCGRG 1 cut(s) 677
Eco91I GGTNACC 1 cut(s) 805
EcoO65I GGTNACC 1 cut(s) 805
EcoRII CCWGG 1 cut(s) 534
EcoT14I CCWWGG 1 cut(s) 297
EcoT38I GRGCYC 1 cut(s) 347
ErhI CCWWGG 1 cut(s) 297
FaeI CATG 2 cut(s) 301, 940
FaiI YATR 8 cut(s) 299, 512, 636, 825, 854, 882, 884, 938
FalI AAGNNNNNCTT 2 cut(s) 890, 922
FaqI GGGAC 3 cut(s) 315, 1178, 1217
FatI CATG 2 cut(s) 297, 936
FauI CCCGC 3 cut(s) 235, 345, 680
Fnu4HI GCNGC 6 cut(s) 166, 391, 532, 563, 685, 945
FokI GGATG 5 cut(s) 79, 551, 617, 755, 912
FriOI GRGCYC 1 cut(s) 347
Fsp4HI GCNGC 6 cut(s) 166, 391, 532, 563, 685, 945
GluI GCNGC 6 cut(s) 166, 391, 532, 563, 685, 945
HaeIII GGCC 3 cut(s) 235, 534, 676
HapII CCGG 3 cut(s) 232, 341, 1201
Hin1II CATG 2 cut(s) 301, 940
HinfI GANTC 9 cut(s) 112, 248, 271, 317, 421, 774, 797, 1001, 1166
HpaII CCGG 3 cut(s) 232, 341, 1201
HphI GGTGA 4 cut(s) 46, 479, 491, 799
Hpy166II GTNNAC 1 cut(s) 557
Hpy188III TCNNGA 6 cut(s) 376, 425, 628, 875, 929, 1020
Hpy8I GTNNAC 1 cut(s) 557
Hpy99I CGWCG 1 cut(s) 398
HpyAV CCTTC 4 cut(s) 469, 492, 892, 896
HpyCH4III ACNGT 1 cut(s) 180
HpyCH4V TGCA 4 cut(s) 455, 463, 947, 1120
HpyF10VI GCNNNNNNNGC 1 cut(s) 396
HpyF3I CTNAG 7 cut(s) 17, 85, 170, 515, 618, 662, 912
Hsp92II CATG 2 cut(s) 301, 940
Kzo9I GATC 3 cut(s) 88, 293, 1050
LmnI GCTCC 1 cut(s) 349
Lsp1109I GCAGC 3 cut(s) 177, 402, 931
LweI GCATC 2 cut(s) 216, 529
MaeIII GTNAC 2 cut(s) 180, 805
MalI GATC 3 cut(s) 90, 295, 1052
MbiI CCGCTC 1 cut(s) 531
MboI GATC 3 cut(s) 88, 293, 1050
MflI RGATCY 1 cut(s) 88
MhlI GDGCHC 1 cut(s) 347
MluCI AATT 4 cut(s) 142, 860, 986, 1246
MlyI GAGTC 3 cut(s) 783, 1010, 1160
MmeI TCCRAC 3 cut(s) 37, 120, 345
MroXI GAANNNNTTC 1 cut(s) 920
MseI TTAA 4 cut(s) 443, 840, 990, 1098
MspA1I CMGCKG 1 cut(s) 944
MspCI CTTAAG 2 cut(s) 442, 1097
MspI CCGG 3 cut(s) 232, 341, 1201
MspR9I CCNGG 4 cut(s) 232, 342, 536, 1202
Mva1269I GAATGC 2 cut(s) 288, 1090
MvaI CCWGG 1 cut(s) 536
MvnI CGCG 1 cut(s) 565
MwoI GCNNNNNNNGC 1 cut(s) 396
NciI CCSGG 3 cut(s) 232, 342, 1202
NcoI CCATGG 1 cut(s) 297
NdeII GATC 3 cut(s) 88, 293, 1050
NlaIII CATG 2 cut(s) 301, 940
NlaIV GGNNCC 1 cut(s) 303
NmeAIII GCCGAG 1 cut(s) 552
NmuCI GTSAC 2 cut(s) 180, 805
NspI RCATGY 1 cut(s) 940
PciI ACATGT 1 cut(s) 936
PcsI WCGNNNNNNNCGW 1 cut(s) 893
PctI GAATGC 2 cut(s) 288, 1090
PdmI GAANNNNTTC 1 cut(s) 920
PfeI GAWTC 6 cut(s) 112, 248, 271, 317, 421, 797
PfoI TCCNGGA 1 cut(s) 1200
PkrI GCNGC 6 cut(s) 167, 392, 533, 564, 686, 946
PleI GAGTC 3 cut(s) 782, 1009, 1160
PpsI GAGTC 3 cut(s) 782, 1009, 1160
PscI ACATGT 1 cut(s) 936
Psp6I CCWGG 1 cut(s) 534
PspEI GGTNACC 1 cut(s) 805
PspGI CCWGG 1 cut(s) 534
PspN4I GGNNCC 1 cut(s) 303
PspPI GGNCC 3 cut(s) 234, 302, 675
PstI CTGCAG 1 cut(s) 949
PsuI RGATCY 1 cut(s) 88
PvuII CAGCTG 1 cut(s) 944
RsaI GTAC 4 cut(s) 257, 1026, 1145, 1229
RsaNI GTAC 4 cut(s) 256, 1025, 1144, 1228
SaqAI TTAA 4 cut(s) 443, 840, 990, 1098
SatI GCNGC 6 cut(s) 166, 391, 532, 563, 685, 945
Sau3AI GATC 3 cut(s) 88, 293, 1050
Sau96I GGNCC 3 cut(s) 234, 302, 675
SchI GAGTC 3 cut(s) 783, 1010, 1160
ScrFI CCNGG 4 cut(s) 232, 342, 536, 1202
SduI GDGCHC 1 cut(s) 347
SfaNI GCATC 2 cut(s) 216, 529
SfcI CTRYAG 1 cut(s) 945
SinI GGWCC 1 cut(s) 302
SmlI CTYRAG 3 cut(s) 442, 790, 1097
SmoI CTYRAG 3 cut(s) 442, 790, 1097
Sse9I AATT 4 cut(s) 142, 860, 986, 1246
StyD4I CCNGG 4 cut(s) 230, 340, 534, 1200
StyI CCWWGG 1 cut(s) 297
TaaI ACNGT 1 cut(s) 180
TaqI TCGA 5 cut(s) 292, 419, 491, 521, 836
TaqII GACCGA 1 cut(s) 747
TasI AATT 4 cut(s) 142, 860, 986, 1246
TatI WGTACW 2 cut(s) 1024, 1227
TauI GCSGC 3 cut(s) 534, 565, 687
TfiI GAWTC 6 cut(s) 112, 248, 271, 317, 421, 797
Tru1I TTAA 4 cut(s) 443, 840, 990, 1098
Tru9I TTAA 4 cut(s) 443, 840, 990, 1098
TscAI CASTG 1 cut(s) 556
TseFI GTSAC 2 cut(s) 180, 805
TseI GCWGC 3 cut(s) 165, 390, 944
Tsp45I GTSAC 2 cut(s) 180, 805
TspDTI ATGAA 1 cut(s) 999
TspRI CASTG 1 cut(s) 556
Vha464I CTTAAG 2 cut(s) 442, 1097
VpaK11BI GGWCC 1 cut(s) 302
XapI RAATTY 1 cut(s) 986
XceI RCATGY 1 cut(s) 940
XmnI GAANNNNTTC 1 cut(s) 920
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.