Rw4G011860

Ribosomal_S15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0001441
Physical Location & Seq
Reverse (-)
28023067 .. 28027429
4363 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G011860.1

Sequence Viewer

Length: 1245 bp
ATGGCTCTCCGACTGAGAACCAACCAGCACCGAACCCTCACAAACCCATCATCTCTCATCCACCTCTTCTCCACTTCCTCCTCAGACCCATCCGACTCAGCAGACCCAACTGAACCCAATCCCCAGTCCGACCCTTCCTCTCTCTCCAACTACTTCAGCGATGTCAAAGCCAGCCTCAGGCAACAGCAACAACACAGGAGCAGACCCACTTCTCAGAACAACAACCCTTCTCTCTCCCGCCCTTCCAAGGCCGCCTCCTTGGAGGAAATCAGCAACAAGCTTGCGGAGTTTCGGCTTCGAAACCCCACTGCACCTCCACCTCCACCTCCGAACCCCATTTCGTTTGAGAAGCTCTATAACAAAGACGTGAGCAGCCGCAAGATGGAGTTTCAGGCGATTCGGGAGAGTCTGAAGCTTATAAAGCCCAATGCCGATGAGCGGAATGAGAGGAAGGGTGAGGGTTTGCCGTTGTTGAGGCTTCGGAAAAGTTTGGATATGAATCCCAGCCAGGATGCGGGTGTTAGGGAGGGAGCACTTCCGAATTTGTTTTATAAGGAGAAGGAGAGAAACGGAGACAAGAGCTCGGATGCATTGAAGACCGAGTTTGTGAAGATTTATAGCTATAAAGAGTTGGGGGAGAAGCTGAGCGAGTTGAGGCCCGAGGAGGGGAAGGAGTTTACATTGGGGGAGTTGAGTGAGAGGTTGGTCAAGTTGAGGATGATGGAAGAGAAAGAGTCTGAGTCTCGGGTTGGGGGAGTGTTTGTGGACGTGAGGCACAGCTTGGAGGCCTTGAAGATATCCCAACAAGAGGAGAAGCATAGGAAAACTTCGAGGCAGAGGCTTCGTATCATAGATATAGGTCAAACTCCTGAGTATATGATGAAACCCCCAAAGGAAGGCTTGGTTGAGAAGTATTTCCATCCAGATAACATGTCTTCTGCAGAAAAGCTGAAAATTGAGCTTGGCAAAGTTAGAGATGAATTTAAAATGTCAGAGTCAGATTGTGGTTCTGCACGAGTTCAAGTGGCACAACTCACAACTAAGATCAAACATCTGTCTTCAGTTTTACACAAAAAGGATAAGCATTCACTTAAGGGTCTTCAAGCAATGGTGCAGAGGAGAAAGAAGCTATTAAAGTATCTTCGAAGAACTGACTGGGACTCTTACTGCCTTGTTCTCTCCAAACTTGGTCTCCGGGACACTCCAGATGTCAAACAAGAGTACAAGAAACATTACAAGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S15

Protein Analysis

414

Amino Acids

47.57

Weight (kDa)

9.62

Isoelectric Point (pI)

53.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S15 PF00312 324 - 399 1.6e-23 Ribosomal protein S15
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 419, 552
AccBSI CCGCTC 1 cut(s) 439
AciI CCGC 6 cut(s) 238, 252, 284, 376, 439, 515
AcsI RAATTY 2 cut(s) 541, 980
AcuI CTGAAG 3 cut(s) 139, 431, 1044
AfaI GTAC 1 cut(s) 1223
AfiI CCNNNNNNNGG 9 cut(s) 177, 247, 382, 438, 514, 665, 666, 808, 896
AflII CTTAAG 1 cut(s) 1091
AflIII ACRYGT 1 cut(s) 930
AgsI TTSAA 4 cut(s) 595, 793, 1022, 1103
AjiI CACGTC 2 cut(s) 367, 769
AjnI CCWGG 1 cut(s) 507
Alw21I GWGCWC 2 cut(s) 535, 584
Alw26I GTCTC 3 cut(s) 567, 747, 1196
Ama87I CYCGRG 2 cut(s) 659, 744
AoxI GGCC 3 cut(s) 249, 656, 786
ApeKI GCWGC 1 cut(s) 372
ApoI RAATTY 2 cut(s) 541, 980
Asp700I GAANNNNTTC 1 cut(s) 914
AspS9I GGNCC 1 cut(s) 657
AsuC2I CCSGG 1 cut(s) 1196
AsuHPI GGTGA 1 cut(s) 467
AsuII TTCGAA 2 cut(s) 298, 1144
AvaI CYCGRG 2 cut(s) 659, 744
AxyI CCTNAGG 1 cut(s) 176
BanII GRGCYC 1 cut(s) 584
BauI CACGAG 1 cut(s) 1014
BbsI GAAGAC 4 cut(s) 602, 927, 1050, 1091
Bbv12I GWGCWC 2 cut(s) 535, 584
BbvI GCAGC 1 cut(s) 384
BccI CCATC 5 cut(s) 55, 97, 376, 715, 927
BceAI ACGGC 1 cut(s) 451
BcgI CGANNNNNNTGC 2 cut(s) 824, 858
BciT130I CCWGG 1 cut(s) 509
BcnI CCSGG 1 cut(s) 1196
BcoDI GTCTC 3 cut(s) 567, 747, 1196
BfmI CTRYAG 1 cut(s) 939
BfrI CTTAAG 1 cut(s) 1091
BisI GCNGC 3 cut(s) 252, 373, 376
BlpI GCTNAGC 1 cut(s) 644
BlsI GCNGC 3 cut(s) 253, 374, 377
Bme1390I CCNGG 2 cut(s) 509, 1196
BmeT110I CYCGRG 2 cut(s) 659, 744
BmgBI CACGTC 2 cut(s) 367, 769
BmgT120I GGNCC 1 cut(s) 657
BmrFI CCNGG 2 cut(s) 509, 1196
BmrI ACTGGG 2 cut(s) 118, 1165
BmsI GCATC 2 cut(s) 502, 577
BmuI ACTGGG 2 cut(s) 118, 1165
BpiI GAAGAC 4 cut(s) 602, 927, 1050, 1091
BpmI CTGGAG 1 cut(s) 1188
Bpu1102I GCTNAGC 1 cut(s) 644
Bpu14I TTCGAA 2 cut(s) 298, 1144
BpuMI CCSGG 1 cut(s) 1196
BsaBI GATNNNNATC 1 cut(s) 498
BsaI GGTCTC 1 cut(s) 1196
BsaJI CCNNGG 3 cut(s) 246, 258, 660
BsaXI ACNNNNNCTCC 8 cut(s) 53, 83, 298, 328, 747, 777, 1176, 1206
Bsc4I CCNNNNNNNGG 9 cut(s) 177, 247, 382, 438, 514, 665, 666, 808, 896
Bse1I ACTGG 2 cut(s) 124, 1160
Bse21I CCTNAGG 1 cut(s) 176
Bse3DI GCAATG 1 cut(s) 1113
Bse8I GATNNNNATC 1 cut(s) 498
BseBI CCWGG 1 cut(s) 509
BseDI CCNNGG 3 cut(s) 246, 258, 660
BseGI GGATG 6 cut(s) 57, 89, 517, 592, 723, 919
BseJI GATNNNNATC 1 cut(s) 498
BseLI CCNNNNNNNGG 9 cut(s) 177, 247, 382, 438, 514, 665, 666, 808, 896
BseMI GCAATG 1 cut(s) 1113
BseMII CTCAG 8 cut(s) 5, 96, 111, 190, 227, 635, 729, 861
BseNI ACTGG 2 cut(s) 124, 1160
BseRI GAGGAG 4 cut(s) 70, 677, 824, 1132
BseXI GCAGC 1 cut(s) 384
BseYI CCCAGC 1 cut(s) 503
BsgI GTGCAG 3 cut(s) 294, 996, 1133
BshFI GGCC 3 cut(s) 251, 658, 788
BsiHKAI GWGCWC 2 cut(s) 535, 584
BsiHKCI CYCGRG 2 cut(s) 659, 744
BsiSI CCGG 1 cut(s) 1195
BslFI GGGAC 2 cut(s) 1172, 1211
BslI CCNNNNNNNGG 9 cut(s) 177, 247, 382, 438, 514, 665, 666, 808, 896
BsmAI GTCTC 3 cut(s) 567, 747, 1196
BsmFI GGGAC 2 cut(s) 1172, 1211
BsmI GAATGC 1 cut(s) 1084
BsnI GGCC 3 cut(s) 251, 658, 788
Bso31I GGTCTC 1 cut(s) 1196
BsoBI CYCGRG 2 cut(s) 659, 744
Bsp119I TTCGAA 2 cut(s) 298, 1144
Bsp1286I GDGCHC 2 cut(s) 535, 584
Bsp143I GATC 1 cut(s) 1044
Bsp1720I GCTNAGC 1 cut(s) 644
BspACI CCGC 6 cut(s) 238, 252, 284, 376, 439, 515
BspANI GGCC 3 cut(s) 251, 658, 788
BspCNI CTCAG 8 cut(s) 6, 95, 110, 189, 226, 636, 730, 862
BspMAI CTGCAG 1 cut(s) 943
BspT104I TTCGAA 2 cut(s) 298, 1144
BspTI CTTAAG 1 cut(s) 1091
BspTNI GGTCTC 1 cut(s) 1196
BsrBI CCGCTC 1 cut(s) 439
BsrDI GCAATG 1 cut(s) 1113
BsrI ACTGG 2 cut(s) 124, 1160
BssECI CCNNGG 3 cut(s) 246, 258, 660
BssMI GATC 1 cut(s) 1044
BssSI CACGAG 1 cut(s) 1014
BssT1I CCWWGG 2 cut(s) 246, 258
Bst2BI CACGAG 1 cut(s) 1014
Bst2UI CCWGG 1 cut(s) 509
Bst6I CTCTTC 2 cut(s) 71, 720
BstAFI CTTAAG 1 cut(s) 1091
BstBI TTCGAA 2 cut(s) 298, 1144
BstC8I GCNNGC 2 cut(s) 172, 282
BstDEI CTNAG 9 cut(s) 14, 82, 97, 176, 213, 644, 738, 870, 1041
BstF5I GGATG 6 cut(s) 57, 89, 517, 592, 723, 919
BstKTI GATC 1 cut(s) 1047
BstMAI GTCTC 3 cut(s) 567, 747, 1196
BstMBI GATC 1 cut(s) 1044
BstMWI GCNNNNNNNGC 1 cut(s) 421
BstNI CCWGG 1 cut(s) 509
BstNSI RCATGY 1 cut(s) 934
BstSCI CCNGG 2 cut(s) 507, 1194
BstSFI CTRYAG 1 cut(s) 939
BstV1I GCAGC 1 cut(s) 384
BstV2I GAAGAC 4 cut(s) 602, 927, 1050, 1091
Bsu36I CCTNAGG 1 cut(s) 176
BsuRI GGCC 3 cut(s) 251, 658, 788
BtgZI GCGATG 1 cut(s) 174
BtrI CACGTC 2 cut(s) 367, 769
BtsCI GGATG 6 cut(s) 57, 89, 517, 592, 723, 919
BtsI GCAGTG 1 cut(s) 306
BtsIMutI CAGTG 1 cut(s) 306
Cac8I GCNNGC 2 cut(s) 172, 282
Cfr13I GGNCC 1 cut(s) 657
Csp6I GTAC 1 cut(s) 1222
CviAII CATG 1 cut(s) 931
CviQI GTAC 1 cut(s) 1222
DdeI CTNAG 9 cut(s) 14, 82, 97, 176, 213, 644, 738, 870, 1041
DpnI GATC 1 cut(s) 1046
DpnII GATC 1 cut(s) 1044
DraI TTTAAA 1 cut(s) 985
Eam1104I CTCTTC 2 cut(s) 71, 720
EarI CTCTTC 2 cut(s) 71, 720
Ecl136II GAGCTC 1 cut(s) 582
Eco130I CCWWGG 2 cut(s) 246, 258
Eco147I AGGCCT 1 cut(s) 788
Eco24I GRGCYC 1 cut(s) 584
Eco31I GGTCTC 1 cut(s) 1196
Eco32I GATATC 1 cut(s) 798
Eco53kI GAGCTC 1 cut(s) 582
Eco57I CTGAAG 3 cut(s) 139, 431, 1044
Eco81I CCTNAGG 1 cut(s) 176
Eco88I CYCGRG 2 cut(s) 659, 744
EcoICRI GAGCTC 1 cut(s) 582
EcoRII CCWGG 1 cut(s) 507
EcoRV GATATC 1 cut(s) 798
EcoT14I CCWWGG 2 cut(s) 246, 258
EcoT22I ATGCAT 1 cut(s) 592
EcoT38I GRGCYC 1 cut(s) 584
ErhI CCWWGG 2 cut(s) 246, 258
FaeI CATG 1 cut(s) 934
FalI AAGNNNNNCTT 2 cut(s) 884, 916
FaqI GGGAC 2 cut(s) 1172, 1211
FatI CATG 1 cut(s) 930
FauI CCCGC 2 cut(s) 245, 508
Fnu4HI GCNGC 3 cut(s) 252, 373, 376
FokI GGATG 6 cut(s) 44, 76, 524, 599, 730, 906
FriOI GRGCYC 1 cut(s) 584
Fsp4HI GCNGC 3 cut(s) 252, 373, 376
GluI GCNGC 3 cut(s) 252, 373, 376
GsaI CCCAGC 1 cut(s) 507
GsuI CTGGAG 1 cut(s) 1188
HaeIII GGCC 3 cut(s) 251, 658, 788
HapII CCGG 1 cut(s) 1195
Hin1II CATG 1 cut(s) 934
HindIII AAGCTT 2 cut(s) 278, 413
HinfI GANTC 8 cut(s) 95, 397, 406, 499, 734, 740, 995, 1160
HpaII CCGG 1 cut(s) 1195
HphI GGTGA 1 cut(s) 467
Hpy166II GTNNAC 2 cut(s) 678, 766
Hpy188III TCNNGA 4 cut(s) 401, 869, 923, 1205
Hpy8I GTNNAC 2 cut(s) 678, 766
HpyAV CCTTC 7 cut(s) 144, 237, 252, 445, 553, 664, 890
HpyCH4IV ACGT 2 cut(s) 366, 768
HpyCH4V TGCA 5 cut(s) 311, 590, 941, 1013, 1114
HpyF10VI GCNNNNNNNGC 1 cut(s) 421
HpyF3I CTNAG 9 cut(s) 14, 82, 97, 176, 213, 644, 738, 870, 1041
HpySE526I ACGT 2 cut(s) 366, 768
Hsp92II CATG 1 cut(s) 934
Kzo9I GATC 1 cut(s) 1044
LmnI GCTCC 2 cut(s) 198, 530
Lsp1109I GCAGC 1 cut(s) 384
LweI GCATC 2 cut(s) 502, 577
MaeII ACGT 2 cut(s) 366, 768
MalI GATC 1 cut(s) 1046
MbiI CCGCTC 1 cut(s) 439
MboI GATC 1 cut(s) 1044
MhlI GDGCHC 2 cut(s) 535, 584
MluCI AATT 4 cut(s) 541, 954, 980, 1240
MlyI GAGTC 6 cut(s) 89, 415, 743, 749, 1004, 1154
MmeI TCCRAC 4 cut(s) 34, 117, 153, 171
Mph1103I ATGCAT 1 cut(s) 592
MroXI GAANNNNTTC 1 cut(s) 914
MseI TTAA 4 cut(s) 984, 1092, 1133, 1243
MspCI CTTAAG 1 cut(s) 1091
MspI CCGG 1 cut(s) 1195
MspR9I CCNGG 2 cut(s) 509, 1196
Mva1269I GAATGC 1 cut(s) 1084
MvaI CCWGG 1 cut(s) 509
MwoI GCNNNNNNNGC 1 cut(s) 421
NciI CCSGG 1 cut(s) 1196
NdeII GATC 1 cut(s) 1044
NlaIII CATG 1 cut(s) 934
NsiI ATGCAT 1 cut(s) 592
NspI RCATGY 1 cut(s) 934
NspV TTCGAA 2 cut(s) 298, 1144
PceI AGGCCT 1 cut(s) 788
PciI ACATGT 1 cut(s) 930
PctI GAATGC 1 cut(s) 1084
PdmI GAANNNNTTC 1 cut(s) 914
PfeI GAWTC 2 cut(s) 397, 499
PfoI TCCNGGA 1 cut(s) 1194
PkrI GCNGC 3 cut(s) 253, 374, 377
PleI GAGTC 6 cut(s) 89, 414, 742, 748, 1003, 1154
PpsI GAGTC 6 cut(s) 89, 414, 742, 748, 1003, 1154
PscI ACATGT 1 cut(s) 930
PsiI TTATAA 2 cut(s) 419, 552
Psp124BI GAGCTC 1 cut(s) 584
Psp6I CCWGG 1 cut(s) 507
PspFI CCCAGC 1 cut(s) 503
PspGI CCWGG 1 cut(s) 507
PspPI GGNCC 1 cut(s) 657
PstI CTGCAG 1 cut(s) 943
RsaI GTAC 1 cut(s) 1223
RsaNI GTAC 1 cut(s) 1222
SacI GAGCTC 1 cut(s) 584
SaqAI TTAA 4 cut(s) 984, 1092, 1133, 1243
SatI GCNGC 3 cut(s) 252, 373, 376
Sau3AI GATC 1 cut(s) 1044
Sau96I GGNCC 1 cut(s) 657
SchI GAGTC 6 cut(s) 89, 415, 743, 749, 1004, 1154
ScrFI CCNGG 2 cut(s) 509, 1196
SduI GDGCHC 2 cut(s) 535, 584
SfaNI GCATC 2 cut(s) 502, 577
SfcI CTRYAG 1 cut(s) 939
SfuI TTCGAA 2 cut(s) 298, 1144
SmlI CTYRAG 1 cut(s) 1091
SmoI CTYRAG 1 cut(s) 1091
Sse9I AATT 4 cut(s) 541, 954, 980, 1240
SseBI AGGCCT 1 cut(s) 788
SsiI CCGC 6 cut(s) 238, 252, 284, 376, 439, 515
SstI GAGCTC 1 cut(s) 584
StuI AGGCCT 1 cut(s) 788
StyD4I CCNGG 2 cut(s) 507, 1194
StyI CCWWGG 2 cut(s) 246, 258
TaiI ACGT 2 cut(s) 369, 771
TaqI TCGA 3 cut(s) 298, 830, 1144
TaqII GACCGA 1 cut(s) 614
TasI AATT 4 cut(s) 541, 954, 980, 1240
TatI WGTACW 1 cut(s) 1221
TauI GCSGC 2 cut(s) 254, 378
TfiI GAWTC 2 cut(s) 397, 499
Tru1I TTAA 4 cut(s) 984, 1092, 1133, 1243
Tru9I TTAA 4 cut(s) 984, 1092, 1133, 1243
TscAI CASTG 1 cut(s) 313
TseI GCWGC 1 cut(s) 372
TspDTI ATGAA 3 cut(s) 512, 896, 993
TspGWI ACGGA 1 cut(s) 585
TspRI CASTG 1 cut(s) 313
Vha464I CTTAAG 1 cut(s) 1091
XapI RAATTY 2 cut(s) 541, 980
XceI RCATGY 1 cut(s) 934
XmnI GAANNNNTTC 1 cut(s) 914
Zsp2I ATGCAT 1 cut(s) 592
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.