Rw2G029760

Ribosomal_S15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0001441
Physical Location & Seq
Reverse (-)
48230063 .. 48234592
4530 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G029760.1

Sequence Viewer

Length: 1353 bp
ATGATGGCTCTCCGACTGAGAACCAACCACCGAACCCTCACAAACCCATCTCTCATTCACCTCTTCTCGACTTCCTCCCCAGACCCATCCGACTCAACCGACCCAACTGAACCCAACCCCCAGTCCGACTCCTCCTCTCTCTCCAATTACTTCAGCGATGTCAAAGCCAGCCTCAGGCAACAGTCACAGTCACAGTCACAGCAACAACACAGCAGACCCACTTCATACTCACAGCAACAACACTTCAGACCCACTTCTCAGAACAACAACCCTTCTCTCTCCCGGCCTTCCAAGGCTGCCTCCTTGGAGGAAATCCGCAAAAACCTTGCGGAGTTTCGGCTTCGATCCATTGGGCCAAACCCCACTGAATCAAACTCTGCACCTCCACCTCCACCTCCACCTCCGAACCCCATTTCGTTTGAGAAGCTCTATAACAAAGGCGTGAGCAGCCGCAAGATGGAGTTTCAGACGGTTCGGGAGAGTCTGAAGCTCATAAGGCCCAATGCGAATGAGCAGAATGAGAGGAAGGGTAGGGGATTGTCGTTGTTGAGGTCCAAAAGTTTGGATATGAATCCGAGCCAGGATGTGGGTGTTAGGAAGGGAGCACTTACAAGTTTGTTTGGTAAGGAGAAGGAGAGAAGTGGAGAGAAGAGCTTGGATGCATTGAAGACTGAGTTTGTGAAGATTTATAGCTATAAAGAGTTGGGGGAGAAGCTGAGGAAGTTGAGGCCCGAGGAAGGGAAGGAAGGGGGCGGGTTTACATTGGGGGAGTTCAGTGAGAGGTTGGTCAAGTTGAGGAAGATGGAAGAGAATGAGTCCGAGTCTTGGGTTGGGGGATTGAATACTAACTTGACGCAGAGCTTGTTGAAGATATCCGCGGAGGAGAAGCATAGGAAAACTCCGATGCAGAGGCTTCATATGATAGATATAGGTCAAACTCCTGATTATATGATGAAACCCCCAAAGGAAGGCTTGGTTGAGAAGTATTTCCATCCAGATAACATGTCTTCTGCAGAAAAGCTGAAAATTGAGCTTGGCAAAGTTAGAGATGAGTTTAAAATGTCAGAGTCAGATTGTGGTTCTGCACGAGTTCAAGTGGCACAACTCACAACTAAGATCAAGCATCTATCTTCAGTTCTACACAAAAAGGATAAGCATTCACTTAAGGGTCTTCAAGCAATGGTGCAGAGGAGGAAAAAGCTATTAAAGTATCTCCGAAGAACTGACTGGGACTCTTACTGCCTTGTTCTCTCCAAACTTGGTCTCCGGGACACGCCAGATGCCAAACAAGAGTACAGAAACATTACAAGAATTAGAGCATTTGAATACGTTTTCTCGTTGTTTCGTCAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S15

Protein Analysis

450

Amino Acids

51.38

Weight (kDa)

9.82

Isoelectric Point (pI)

54.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S15 PF00312 348 - 423 1.9e-23 Ribosomal protein S15
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 586
AccII CGCG 1 cut(s) 878
AciI CCGC 6 cut(s) 316, 329, 451, 753, 876, 878
AclWI GGATC 1 cut(s) 339
AcuI CTGAAG 4 cut(s) 136, 229, 506, 1116
AfaI GTAC 1 cut(s) 1295
AfiI CCNNNNNNNGG 7 cut(s) 174, 457, 586, 737, 738, 825, 968
AflII CTTAAG 1 cut(s) 1163
AflIII ACRYGT 1 cut(s) 1002
AgsI TTSAA 6 cut(s) 667, 841, 868, 1094, 1175, 1325
AjnI CCWGG 1 cut(s) 579
AluBI AGCT 9 cut(s) 427, 490, 654, 693, 715, 861, 1021, 1033, 1201
AluI AGCT 9 cut(s) 427, 490, 654, 693, 715, 861, 1021, 1033, 1201
Alw21I GWGCWC 1 cut(s) 607
Alw26I GTCTC 1 cut(s) 1268
AlwI GGATC 1 cut(s) 339
Ama87I CYCGRG 1 cut(s) 731
AoxI GGCC 4 cut(s) 284, 353, 497, 728
ApeKI GCWGC 2 cut(s) 296, 447
Asp700I GAANNNNTTC 1 cut(s) 986
AspS9I GGNCC 4 cut(s) 353, 498, 552, 729
AsuC2I CCSGG 2 cut(s) 283, 1268
AsuHPI GGTGA 1 cut(s) 50
AvaI CYCGRG 1 cut(s) 731
AvaII GGWCC 1 cut(s) 552
AxyI CCTNAGG 1 cut(s) 173
BauI CACGAG 1 cut(s) 1086
BbsI GAAGAC 3 cut(s) 674, 999, 1163
Bbv12I GWGCWC 1 cut(s) 607
BbvCI CCTCAGC 1 cut(s) 716
BbvI GCAGC 2 cut(s) 283, 459
BccI CCATC 5 cut(s) 55, 94, 451, 796, 999
BciT130I CCWGG 1 cut(s) 581
BcnI CCSGG 2 cut(s) 283, 1268
BcoDI GTCTC 1 cut(s) 1268
BfmI CTRYAG 1 cut(s) 1011
BfrI CTTAAG 1 cut(s) 1163
BisI GCNGC 3 cut(s) 297, 448, 451
BlsI GCNGC 3 cut(s) 298, 449, 452
Bme1390I CCNGG 3 cut(s) 283, 581, 1268
Bme18I GGWCC 1 cut(s) 552
BmeT110I CYCGRG 1 cut(s) 731
BmgT120I GGNCC 4 cut(s) 353, 498, 552, 729
BmrFI CCNGG 3 cut(s) 283, 581, 1268
BmrI ACTGGG 2 cut(s) 115, 1237
BmsI GCATC 4 cut(s) 649, 894, 1132, 1270
BmuI ACTGGG 2 cut(s) 115, 1237
BpiI GAAGAC 3 cut(s) 674, 999, 1163
Bpu10I CCTNAGC 1 cut(s) 716
BpuMI CCSGG 2 cut(s) 283, 1268
BsaBI GATNNNNATC 1 cut(s) 570
BsaI GGTCTC 1 cut(s) 1268
BsaJI CCNNGG 4 cut(s) 291, 303, 732, 876
BsaXI ACNNNNNCTCC 2 cut(s) 1248, 1278
Bsc4I CCNNNNNNNGG 7 cut(s) 174, 457, 586, 737, 738, 825, 968
Bse1I ACTGG 2 cut(s) 121, 1232
Bse21I CCTNAGG 1 cut(s) 173
Bse3DI GCAATG 1 cut(s) 1185
Bse8I GATNNNNATC 1 cut(s) 570
BseBI CCWGG 1 cut(s) 581
BseDI CCNNGG 4 cut(s) 291, 303, 732, 876
BseGI GGATG 4 cut(s) 86, 589, 664, 991
BseJI GATNNNNATC 1 cut(s) 570
BseLI CCNNNNNNNGG 7 cut(s) 174, 457, 586, 737, 738, 825, 968
BseMI GCAATG 1 cut(s) 1185
BseMII CTCAG 5 cut(s) 8, 187, 272, 663, 707
BseNI ACTGG 2 cut(s) 121, 1232
BseRI GAGGAG 4 cut(s) 121, 124, 896, 1204
BseXI GCAGC 2 cut(s) 283, 459
BsgI GTGCAG 3 cut(s) 363, 1068, 1205
Bsh1236I CGCG 1 cut(s) 878
BshFI GGCC 4 cut(s) 286, 355, 499, 730
BsiHKAI GWGCWC 1 cut(s) 607
BsiHKCI CYCGRG 1 cut(s) 731
BsiSI CCGG 2 cut(s) 283, 1267
BslFI GGGAC 2 cut(s) 1244, 1283
BslI CCNNNNNNNGG 7 cut(s) 174, 457, 586, 737, 738, 825, 968
BsmAI GTCTC 1 cut(s) 1268
BsmFI GGGAC 2 cut(s) 1244, 1283
BsmI GAATGC 1 cut(s) 1156
BsnI GGCC 4 cut(s) 286, 355, 499, 730
Bso31I GGTCTC 1 cut(s) 1268
BsoBI CYCGRG 1 cut(s) 731
Bsp1286I GDGCHC 1 cut(s) 607
Bsp143I GATC 2 cut(s) 344, 1116
BspACI CCGC 6 cut(s) 316, 329, 451, 753, 876, 878
BspANI GGCC 4 cut(s) 286, 355, 499, 730
BspCNI CTCAG 5 cut(s) 9, 186, 271, 664, 708
BspFNI CGCG 1 cut(s) 878
BspMAI CTGCAG 1 cut(s) 1015
BspPI GGATC 1 cut(s) 339
BspQI GCTCTTC 1 cut(s) 644
BspTI CTTAAG 1 cut(s) 1163
BspTNI GGTCTC 1 cut(s) 1268
BsrDI GCAATG 1 cut(s) 1185
BsrI ACTGG 2 cut(s) 121, 1232
BssECI CCNNGG 4 cut(s) 291, 303, 732, 876
BssMI GATC 2 cut(s) 344, 1116
BssSI CACGAG 1 cut(s) 1086
BssT1I CCWWGG 2 cut(s) 291, 303
Bst2BI CACGAG 1 cut(s) 1086
Bst2UI CCWGG 1 cut(s) 581
Bst4CI ACNGT 4 cut(s) 183, 189, 195, 472
Bst6I CTCTTC 3 cut(s) 68, 644, 801
BstAFI CTTAAG 1 cut(s) 1163
BstC8I GCNNGC 1 cut(s) 169
BstDEI CTNAG 6 cut(s) 17, 173, 258, 672, 716, 1113
BstDSI CCRYGG 1 cut(s) 876
BstF5I GGATG 4 cut(s) 86, 589, 664, 991
BstFNI CGCG 1 cut(s) 878
BstKTI GATC 2 cut(s) 347, 1119
BstMAI GTCTC 1 cut(s) 1268
BstMBI GATC 2 cut(s) 344, 1116
BstMWI GCNNNNNNNGC 2 cut(s) 447, 496
BstNI CCWGG 1 cut(s) 581
BstNSI RCATGY 1 cut(s) 1006
BstSCI CCNGG 3 cut(s) 281, 579, 1266
BstSFI CTRYAG 1 cut(s) 1011
BstUI CGCG 1 cut(s) 878
BstV1I GCAGC 2 cut(s) 283, 459
BstV2I GAAGAC 3 cut(s) 674, 999, 1163
BstXI CCANNNNNNTGG 1 cut(s) 562
Bsu36I CCTNAGG 1 cut(s) 173
BsuRI GGCC 4 cut(s) 286, 355, 499, 730
BtgI CCRYGG 1 cut(s) 876
BtgZI GCGATG 1 cut(s) 171
BtsCI GGATG 4 cut(s) 86, 589, 664, 991
BtsIMutI CAGTG 2 cut(s) 363, 781
Cac8I GCNNGC 1 cut(s) 169
Cfr13I GGNCC 4 cut(s) 353, 498, 552, 729
Cfr42I CCGCGG 1 cut(s) 879
CseI GACGC 1 cut(s) 862
Csp6I GTAC 1 cut(s) 1294
CviAII CATG 1 cut(s) 1003
CviQI GTAC 1 cut(s) 1294
DdeI CTNAG 6 cut(s) 17, 173, 258, 672, 716, 1113
DpnI GATC 2 cut(s) 346, 1118
DpnII GATC 2 cut(s) 344, 1116
DraI TTTAAA 1 cut(s) 1057
Eam1104I CTCTTC 3 cut(s) 68, 644, 801
EarI CTCTTC 3 cut(s) 68, 644, 801
Eco130I CCWWGG 2 cut(s) 291, 303
Eco31I GGTCTC 1 cut(s) 1268
Eco32I GATATC 1 cut(s) 873
Eco47I GGWCC 1 cut(s) 552
Eco57I CTGAAG 4 cut(s) 136, 229, 506, 1116
Eco81I CCTNAGG 1 cut(s) 173
Eco88I CYCGRG 1 cut(s) 731
EcoRII CCWGG 1 cut(s) 579
EcoRV GATATC 1 cut(s) 873
EcoT14I CCWWGG 2 cut(s) 291, 303
EcoT22I ATGCAT 1 cut(s) 664
ErhI CCWWGG 2 cut(s) 291, 303
FaeI CATG 1 cut(s) 1006
FalI AAGNNNNNCTT 2 cut(s) 956, 988
FaqI GGGAC 2 cut(s) 1244, 1283
FatI CATG 1 cut(s) 1002
FauI CCCGC 1 cut(s) 746
FauNDI CATATG 1 cut(s) 918
Fnu4HI GCNGC 3 cut(s) 297, 448, 451
FokI GGATG 4 cut(s) 73, 596, 671, 978
Fsp4HI GCNGC 3 cut(s) 297, 448, 451
GluI GCNGC 3 cut(s) 297, 448, 451
HaeIII GGCC 4 cut(s) 286, 355, 499, 730
HapII CCGG 2 cut(s) 283, 1267
HgaI GACGC 1 cut(s) 862
Hin1II CATG 1 cut(s) 1006
HinfI GANTC 9 cut(s) 92, 128, 368, 481, 571, 815, 821, 1067, 1232
HpaII CCGG 2 cut(s) 283, 1267
HphI GGTGA 1 cut(s) 50
Hpy166II GTNNAC 1 cut(s) 759
Hpy188III TCNNGA 4 cut(s) 67, 476, 941, 995
Hpy8I GTNNAC 1 cut(s) 759
HpyAV CCTTC 9 cut(s) 282, 297, 520, 592, 625, 731, 736, 740, 962
HpyCH4III ACNGT 4 cut(s) 183, 189, 195, 472
HpyCH4IV ACGT 1 cut(s) 1329
HpyCH4V TGCA 6 cut(s) 380, 662, 907, 1013, 1085, 1186
HpyF10VI GCNNNNNNNGC 2 cut(s) 447, 496
HpyF3I CTNAG 6 cut(s) 17, 173, 258, 672, 716, 1113
HpySE526I ACGT 1 cut(s) 1329
Hsp92II CATG 1 cut(s) 1006
KspI CCGCGG 1 cut(s) 879
Kzo9I GATC 2 cut(s) 344, 1116
LguI GCTCTTC 1 cut(s) 644
LmnI GCTCC 1 cut(s) 602
Lsp1109I GCAGC 2 cut(s) 283, 459
LweI GCATC 4 cut(s) 649, 894, 1132, 1270
MaeII ACGT 1 cut(s) 1329
MaeIII GTNAC 3 cut(s) 183, 189, 195
MalI GATC 2 cut(s) 346, 1118
MboI GATC 2 cut(s) 344, 1116
MhlI GDGCHC 1 cut(s) 607
MluCI AATT 3 cut(s) 145, 1026, 1311
MlyI GAGTC 7 cut(s) 86, 122, 490, 824, 830, 1076, 1226
MmeI TCCRAC 3 cut(s) 37, 114, 150
Mph1103I ATGCAT 1 cut(s) 664
MroXI GAANNNNTTC 1 cut(s) 986
MseI TTAA 3 cut(s) 1056, 1164, 1205
MspA1I CMGCKG 1 cut(s) 878
MspCI CTTAAG 1 cut(s) 1163
MspI CCGG 2 cut(s) 283, 1267
MspR9I CCNGG 3 cut(s) 283, 581, 1268
Mva1269I GAATGC 1 cut(s) 1156
MvaI CCWGG 1 cut(s) 581
MvnI CGCG 1 cut(s) 878
MwoI GCNNNNNNNGC 2 cut(s) 447, 496
NciI CCSGG 2 cut(s) 283, 1268
NdeI CATATG 1 cut(s) 918
NdeII GATC 2 cut(s) 344, 1116
NlaIII CATG 1 cut(s) 1006
NmuCI GTSAC 3 cut(s) 183, 189, 195
NsiI ATGCAT 1 cut(s) 664
NspI RCATGY 1 cut(s) 1006
PciI ACATGT 1 cut(s) 1002
PciSI GCTCTTC 1 cut(s) 644
PctI GAATGC 1 cut(s) 1156
PdmI GAANNNNTTC 1 cut(s) 986
PfeI GAWTC 2 cut(s) 368, 571
PflMI CCANNNNNTGG 1 cut(s) 586
PfoI TCCNGGA 1 cut(s) 1266
PkrI GCNGC 3 cut(s) 298, 449, 452
PleI GAGTC 7 cut(s) 86, 122, 489, 823, 829, 1075, 1226
PpsI GAGTC 7 cut(s) 86, 122, 489, 823, 829, 1075, 1226
PscI ACATGT 1 cut(s) 1002
Psp6I CCWGG 1 cut(s) 579
PspGI CCWGG 1 cut(s) 579
PspPI GGNCC 4 cut(s) 353, 498, 552, 729
PstI CTGCAG 1 cut(s) 1015
RsaI GTAC 1 cut(s) 1295
RsaNI GTAC 1 cut(s) 1294
SacII CCGCGG 1 cut(s) 879
SapI GCTCTTC 1 cut(s) 644
SaqAI TTAA 3 cut(s) 1056, 1164, 1205
SatI GCNGC 3 cut(s) 297, 448, 451
Sau3AI GATC 2 cut(s) 344, 1116
Sau96I GGNCC 4 cut(s) 353, 498, 552, 729
SchI GAGTC 7 cut(s) 86, 122, 490, 824, 830, 1076, 1226
ScrFI CCNGG 3 cut(s) 283, 581, 1268
SduI GDGCHC 1 cut(s) 607
SfaNI GCATC 4 cut(s) 649, 894, 1132, 1270
SfcI CTRYAG 1 cut(s) 1011
Sfr303I CCGCGG 1 cut(s) 879
SgrBI CCGCGG 1 cut(s) 879
SinI GGWCC 1 cut(s) 552
SmlI CTYRAG 1 cut(s) 1163
SmoI CTYRAG 1 cut(s) 1163
Sse9I AATT 3 cut(s) 145, 1026, 1311
SsiI CCGC 6 cut(s) 316, 329, 451, 753, 876, 878
StyD4I CCNGG 3 cut(s) 281, 579, 1266
StyI CCWWGG 2 cut(s) 291, 303
TaaI ACNGT 4 cut(s) 183, 189, 195, 472
TaiI ACGT 1 cut(s) 1332
TaqI TCGA 2 cut(s) 68, 343
TasI AATT 3 cut(s) 145, 1026, 1311
TatI WGTACW 1 cut(s) 1293
TauI GCSGC 1 cut(s) 453
TfiI GAWTC 2 cut(s) 368, 571
Tru1I TTAA 3 cut(s) 1056, 1164, 1205
Tru9I TTAA 3 cut(s) 1056, 1164, 1205
TscAI CASTG 2 cut(s) 370, 781
TseFI GTSAC 3 cut(s) 183, 189, 195
TseI GCWGC 2 cut(s) 296, 447
Tsp45I GTSAC 3 cut(s) 183, 189, 195
TspDTI ATGAA 4 cut(s) 213, 584, 905, 968
TspRI CASTG 2 cut(s) 370, 781
Van91I CCANNNNNTGG 1 cut(s) 586
Vha464I CTTAAG 1 cut(s) 1163
VpaK11BI GGWCC 1 cut(s) 552
XceI RCATGY 1 cut(s) 1006
XmnI GAANNNNTTC 1 cut(s) 986
Zsp2I ATGCAT 1 cut(s) 664
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.