Rroxscaffold_2G00111010

Ribosomal_S15

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0001441
Physical Location & Seq
Forward (+)
35537379 .. 35542225
4847 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00111010.1

Sequence Viewer

Length: 1317 bp
ATGAGGGCTCTCCGACTGAGAACCAACCACCGAACCCTCACAAACCCATCTCTCATTCACCTCTTCTCGACTTCCTCCTCAGACCCGTCCGACTCAACCGACCCAACTGAACCCAACCCCCAGTCCGACTCCTCCTCTCTCTCCAATTACTTCAGCGATGTCAAAGCCAGCCTCAGGCAACAGTCACAGTCACAGCAACAACACAGCAGACCCACTTCATACTCACAGCAACAACACTTCAGACCCACTTCTCAGAACAACAACCCTTCTCTCTCCCGGCCTTCCAAGGCTGCCTCCTTGGAGGAAATCCGCAAAAACCTTGCGGAGTTTCGGCTTCGATCCATTGGGCCAAACCCCACTGAATCAAACTCTGCACCTCCACCTCCACCTCCACCTTCACCTCCGAACCCCATTTCGTTTGAGAAGCTCTATAACAAAGGCGTGAGCAGCCGCAAGATGGAGTTTCAGACGGTTCGGGAGAGTCTGAAGCTCATAAGGCCCAATGCGAATGAGCAGAATGAGAGGAAGGGTGGGGGATTGTCGTTGTTGAGGCGCAAAAGTCTGGATATGAATCTGAGCCAGGATGTGGGTGTTAGGAAGGAAGCACTTACAAGTTTGTTTGATAAGGAGAAGGAGAGAAGTGGAGAGAAGAGCTCGGATGCATTGAAGACTGAGTTTGTGAAGATTTATAGCTATAAAGAGTTGGGGGAGAAGCTGAGGAAGTTGAGGCCCGAGGAAGGGAAGGAAGGGGGCGGGTTTACATTGGGGGAGTTCAGTGAGAGGTTGGTCAAGTTGAGGAAGATGGAAGAGAATGAGTCCGAGTCTTGGGTTGGGGGATTCAATACTGACTTGAAGCAGAGCTTGTTGAAGATATCCGGAGAGGAGAAGCATAGGAAAACTCCGACGCAGAGGCTTCATATGATAGATATAGGTCAAACTCCTGATTATATGGTGAAACCCCCAAAGGAAGACTTGGTTGAGAAGTATTTCCATCCAGATAACATGTCTTCTGCAGAAAATCTGAAAATTGAGCTTGGCAAAGTTAGAGTTGAATTTAAAATGTCAGAGTCAGATTGTGGTTCTGCACGAGTTCAAGTGGCACAACTCACAACTAAGATCAAGCATCTATCTTCAGTTCTACACAAAAAGGATAAGCATTCACTTAAGGGTCTTCAAGCAATGGTGCAGAGGAGGAAGAAGCTATTAAAGTATCTCCGAAGAACTGACTGGGACTCTTACTGCCTTGTTCTCTCCAAACTTGGTCTCCGGGACACGCCAGATGCCAAACAAGAGTACAAGAAACATTACAAGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_S15

Protein Analysis

438

Amino Acids

49.97

Weight (kDa)

9.72

Isoelectric Point (pI)

58.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S15 PF00312 349 - 423 3.9e-23 Ribosomal protein S15
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 586
AccIII TCCGGA 1 cut(s) 875
AciI CCGC 4 cut(s) 310, 323, 451, 753
AclWI GGATC 1 cut(s) 333
AcsI RAATTY 1 cut(s) 1052
AcuI CTGAAG 4 cut(s) 136, 223, 506, 1116
AfaI GTAC 1 cut(s) 1295
AfiI CCNNNNNNNGG 6 cut(s) 174, 457, 586, 737, 738, 825
AflII CTTAAG 1 cut(s) 1163
AflIII ACRYGT 1 cut(s) 1002
AgsI TTSAA 7 cut(s) 667, 841, 853, 868, 1052, 1094, 1175
AjnI CCWGG 1 cut(s) 579
AluBI AGCT 8 cut(s) 427, 490, 654, 693, 715, 861, 1033, 1201
AluI AGCT 8 cut(s) 427, 490, 654, 693, 715, 861, 1033, 1201
Alw21I GWGCWC 1 cut(s) 656
Alw26I GTCTC 1 cut(s) 1268
AlwI GGATC 1 cut(s) 333
Ama87I CYCGRG 1 cut(s) 731
Aor13HI TCCGGA 1 cut(s) 875
AoxI GGCC 4 cut(s) 278, 347, 497, 728
ApeKI GCWGC 2 cut(s) 290, 447
ApoI RAATTY 1 cut(s) 1052
Asp700I GAANNNNTTC 1 cut(s) 986
AspLEI GCGC 1 cut(s) 555
AspS9I GGNCC 3 cut(s) 347, 498, 729
AsuC2I CCSGG 2 cut(s) 277, 1268
AsuHPI GGTGA 3 cut(s) 50, 390, 964
AvaI CYCGRG 1 cut(s) 731
AxyI CCTNAGG 1 cut(s) 173
BanII GRGCYC 2 cut(s) 10, 656
BauI CACGAG 1 cut(s) 1086
BbsI GAAGAC 4 cut(s) 674, 975, 999, 1163
Bbv12I GWGCWC 1 cut(s) 656
BbvCI CCTCAGC 1 cut(s) 716
BbvI GCAGC 2 cut(s) 277, 459
BccI CCATC 4 cut(s) 55, 451, 796, 999
BciT130I CCWGG 1 cut(s) 581
BcnI CCSGG 2 cut(s) 277, 1268
BcoDI GTCTC 1 cut(s) 1268
BfmI CTRYAG 1 cut(s) 1011
BfrI CTTAAG 1 cut(s) 1163
BisI GCNGC 3 cut(s) 291, 448, 451
BlsI GCNGC 3 cut(s) 292, 449, 452
Bme1390I CCNGG 3 cut(s) 277, 581, 1268
BmeT110I CYCGRG 1 cut(s) 731
BmgT120I GGNCC 3 cut(s) 347, 498, 729
BmrFI CCNGG 3 cut(s) 277, 581, 1268
BmrI ACTGGG 2 cut(s) 115, 1237
BmsI GCATC 3 cut(s) 649, 1132, 1270
BmuI ACTGGG 2 cut(s) 115, 1237
BpiI GAAGAC 4 cut(s) 674, 975, 999, 1163
BplI GAGNNNNNCTC 2 cut(s) 638, 670
Bpu10I CCTNAGC 1 cut(s) 716
BpuMI CCSGG 2 cut(s) 277, 1268
BsaBI GATNNNNATC 1 cut(s) 570
BsaI GGTCTC 1 cut(s) 1268
BsaJI CCNNGG 3 cut(s) 285, 297, 732
BsaWI WCCGGW 1 cut(s) 875
BsaXI ACNNNNNCTCC 2 cut(s) 1248, 1278
Bsc4I CCNNNNNNNGG 6 cut(s) 174, 457, 586, 737, 738, 825
Bse1I ACTGG 2 cut(s) 121, 1232
Bse21I CCTNAGG 1 cut(s) 173
Bse3DI GCAATG 1 cut(s) 1185
Bse8I GATNNNNATC 1 cut(s) 570
BseAI TCCGGA 1 cut(s) 875
BseBI CCWGG 1 cut(s) 581
BseDI CCNNGG 3 cut(s) 285, 297, 732
BseGI GGATG 3 cut(s) 589, 664, 991
BseJI GATNNNNATC 1 cut(s) 570
BseLI CCNNNNNNNGG 6 cut(s) 174, 457, 586, 737, 738, 825
BseMI GCAATG 1 cut(s) 1185
BseMII CTCAG 7 cut(s) 8, 93, 187, 266, 566, 663, 707
BseNI ACTGG 2 cut(s) 121, 1232
BseRI GAGGAG 5 cut(s) 67, 121, 124, 896, 1204
BseXI GCAGC 2 cut(s) 277, 459
BsgI GTGCAG 3 cut(s) 357, 1068, 1205
BshFI GGCC 4 cut(s) 280, 349, 499, 730
BsiHKAI GWGCWC 1 cut(s) 656
BsiHKCI CYCGRG 1 cut(s) 731
BsiSI CCGG 3 cut(s) 277, 876, 1267
BslFI GGGAC 2 cut(s) 1244, 1283
BslI CCNNNNNNNGG 6 cut(s) 174, 457, 586, 737, 738, 825
BsmAI GTCTC 1 cut(s) 1268
BsmFI GGGAC 2 cut(s) 1244, 1283
BsmI GAATGC 1 cut(s) 1156
BsnI GGCC 4 cut(s) 280, 349, 499, 730
Bso31I GGTCTC 1 cut(s) 1268
BsoBI CYCGRG 1 cut(s) 731
Bsp1286I GDGCHC 2 cut(s) 10, 656
Bsp13I TCCGGA 1 cut(s) 875
Bsp143I GATC 2 cut(s) 338, 1116
BspACI CCGC 4 cut(s) 310, 323, 451, 753
BspANI GGCC 4 cut(s) 280, 349, 499, 730
BspCNI CTCAG 7 cut(s) 9, 92, 186, 265, 567, 664, 708
BspEI TCCGGA 1 cut(s) 875
BspMAI CTGCAG 1 cut(s) 1015
BspPI GGATC 1 cut(s) 333
BspQI GCTCTTC 1 cut(s) 644
BspTI CTTAAG 1 cut(s) 1163
BspTNI GGTCTC 1 cut(s) 1268
BsrDI GCAATG 1 cut(s) 1185
BsrI ACTGG 2 cut(s) 121, 1232
BssECI CCNNGG 3 cut(s) 285, 297, 732
BssMI GATC 2 cut(s) 338, 1116
BssSI CACGAG 1 cut(s) 1086
BssT1I CCWWGG 2 cut(s) 285, 297
Bst2BI CACGAG 1 cut(s) 1086
Bst2UI CCWGG 1 cut(s) 581
Bst4CI ACNGT 3 cut(s) 183, 189, 472
Bst6I CTCTTC 3 cut(s) 68, 644, 801
BstAFI CTTAAG 1 cut(s) 1163
BstC8I GCNNGC 1 cut(s) 169
BstDEI CTNAG 8 cut(s) 17, 79, 173, 252, 575, 672, 716, 1113
BstF5I GGATG 3 cut(s) 589, 664, 991
BstHHI GCGC 1 cut(s) 555
BstKTI GATC 2 cut(s) 341, 1119
BstMAI GTCTC 1 cut(s) 1268
BstMBI GATC 2 cut(s) 338, 1116
BstMWI GCNNNNNNNGC 2 cut(s) 447, 496
BstNI CCWGG 1 cut(s) 581
BstNSI RCATGY 1 cut(s) 1006
BstSCI CCNGG 3 cut(s) 275, 579, 1266
BstSFI CTRYAG 1 cut(s) 1011
BstV1I GCAGC 2 cut(s) 277, 459
BstV2I GAAGAC 4 cut(s) 674, 975, 999, 1163
Bsu36I CCTNAGG 1 cut(s) 173
BsuRI GGCC 4 cut(s) 280, 349, 499, 730
BtgZI GCGATG 1 cut(s) 171
BtsCI GGATG 3 cut(s) 589, 664, 991
BtsIMutI CAGTG 2 cut(s) 357, 781
Cac8I GCNNGC 1 cut(s) 169
CfoI GCGC 1 cut(s) 555
Cfr13I GGNCC 3 cut(s) 347, 498, 729
CseI GACGC 1 cut(s) 913
Csp6I GTAC 1 cut(s) 1294
CviAII CATG 1 cut(s) 1003
CviQI GTAC 1 cut(s) 1294
DdeI CTNAG 8 cut(s) 17, 79, 173, 252, 575, 672, 716, 1113
DpnI GATC 2 cut(s) 340, 1118
DpnII GATC 2 cut(s) 338, 1116
DraI TTTAAA 1 cut(s) 1057
Eam1104I CTCTTC 3 cut(s) 68, 644, 801
EarI CTCTTC 3 cut(s) 68, 644, 801
Ecl136II GAGCTC 1 cut(s) 654
Eco130I CCWWGG 2 cut(s) 285, 297
Eco24I GRGCYC 2 cut(s) 10, 656
Eco31I GGTCTC 1 cut(s) 1268
Eco32I GATATC 1 cut(s) 873
Eco53kI GAGCTC 1 cut(s) 654
Eco57I CTGAAG 4 cut(s) 136, 223, 506, 1116
Eco81I CCTNAGG 1 cut(s) 173
Eco88I CYCGRG 1 cut(s) 731
EcoICRI GAGCTC 1 cut(s) 654
EcoRII CCWGG 1 cut(s) 579
EcoRV GATATC 1 cut(s) 873
EcoT14I CCWWGG 2 cut(s) 285, 297
EcoT22I ATGCAT 1 cut(s) 664
EcoT38I GRGCYC 2 cut(s) 10, 656
ErhI CCWWGG 2 cut(s) 285, 297
FaeI CATG 1 cut(s) 1006
FalI AAGNNNNNCTT 4 cut(s) 845, 877, 956, 988
FaqI GGGAC 2 cut(s) 1244, 1283
FatI CATG 1 cut(s) 1002
FauI CCCGC 1 cut(s) 746
FauNDI CATATG 1 cut(s) 918
Fnu4HI GCNGC 3 cut(s) 291, 448, 451
FokI GGATG 3 cut(s) 596, 671, 978
FriOI GRGCYC 2 cut(s) 10, 656
Fsp4HI GCNGC 3 cut(s) 291, 448, 451
GlaI GCGC 1 cut(s) 554
GluI GCNGC 3 cut(s) 291, 448, 451
HaeIII GGCC 4 cut(s) 280, 349, 499, 730
HapII CCGG 3 cut(s) 277, 876, 1267
HgaI GACGC 1 cut(s) 913
HhaI GCGC 1 cut(s) 555
Hin1II CATG 1 cut(s) 1006
Hin6I GCGC 1 cut(s) 553
HinP1I GCGC 1 cut(s) 553
HpaII CCGG 3 cut(s) 277, 876, 1267
HphI GGTGA 3 cut(s) 50, 390, 964
Hpy166II GTNNAC 1 cut(s) 759
Hpy188III TCNNGA 6 cut(s) 67, 476, 563, 876, 941, 995
Hpy8I GTNNAC 1 cut(s) 759
Hpy99I CGWCG 1 cut(s) 907
HpyAV CCTTC 9 cut(s) 276, 291, 405, 520, 592, 625, 731, 736, 740
HpyCH4III ACNGT 3 cut(s) 183, 189, 472
HpyCH4V TGCA 5 cut(s) 374, 662, 1013, 1085, 1186
HpyF10VI GCNNNNNNNGC 2 cut(s) 447, 496
HpyF3I CTNAG 8 cut(s) 17, 79, 173, 252, 575, 672, 716, 1113
Hsp92II CATG 1 cut(s) 1006
HspAI GCGC 1 cut(s) 553
Kpn2I TCCGGA 1 cut(s) 875
Kzo9I GATC 2 cut(s) 338, 1116
LguI GCTCTTC 1 cut(s) 644
Lsp1109I GCAGC 2 cut(s) 277, 459
LweI GCATC 3 cut(s) 649, 1132, 1270
MaeIII GTNAC 2 cut(s) 183, 189
MalI GATC 2 cut(s) 340, 1118
MboI GATC 2 cut(s) 338, 1116
MhlI GDGCHC 2 cut(s) 10, 656
MluCI AATT 4 cut(s) 145, 1026, 1052, 1312
MlyI GAGTC 7 cut(s) 86, 122, 490, 824, 830, 1076, 1226
MmeI TCCRAC 4 cut(s) 37, 114, 150, 926
Mph1103I ATGCAT 1 cut(s) 664
MroI TCCGGA 1 cut(s) 875
MroXI GAANNNNTTC 1 cut(s) 986
MseI TTAA 3 cut(s) 1056, 1164, 1205
MspCI CTTAAG 1 cut(s) 1163
MspI CCGG 3 cut(s) 277, 876, 1267
MspR9I CCNGG 3 cut(s) 277, 581, 1268
Mva1269I GAATGC 1 cut(s) 1156
MvaI CCWGG 1 cut(s) 581
MwoI GCNNNNNNNGC 2 cut(s) 447, 496
NciI CCSGG 2 cut(s) 277, 1268
NdeI CATATG 1 cut(s) 918
NdeII GATC 2 cut(s) 338, 1116
NlaIII CATG 1 cut(s) 1006
NmuCI GTSAC 2 cut(s) 183, 189
NsiI ATGCAT 1 cut(s) 664
NspI RCATGY 1 cut(s) 1006
PciI ACATGT 1 cut(s) 1002
PciSI GCTCTTC 1 cut(s) 644
PctI GAATGC 1 cut(s) 1156
PdmI GAANNNNTTC 1 cut(s) 986
PfeI GAWTC 3 cut(s) 362, 571, 837
PflMI CCANNNNNTGG 1 cut(s) 586
PfoI TCCNGGA 1 cut(s) 1266
PkrI GCNGC 3 cut(s) 292, 449, 452
PleI GAGTC 7 cut(s) 86, 122, 489, 823, 829, 1075, 1226
PpsI GAGTC 7 cut(s) 86, 122, 489, 823, 829, 1075, 1226
PscI ACATGT 1 cut(s) 1002
Psp124BI GAGCTC 1 cut(s) 656
Psp6I CCWGG 1 cut(s) 579
PspGI CCWGG 1 cut(s) 579
PspPI GGNCC 3 cut(s) 347, 498, 729
PstI CTGCAG 1 cut(s) 1015
RsaI GTAC 1 cut(s) 1295
RsaNI GTAC 1 cut(s) 1294
SacI GAGCTC 1 cut(s) 656
SapI GCTCTTC 1 cut(s) 644
SaqAI TTAA 3 cut(s) 1056, 1164, 1205
SatI GCNGC 3 cut(s) 291, 448, 451
Sau3AI GATC 2 cut(s) 338, 1116
Sau96I GGNCC 3 cut(s) 347, 498, 729
SchI GAGTC 7 cut(s) 86, 122, 490, 824, 830, 1076, 1226
ScrFI CCNGG 3 cut(s) 277, 581, 1268
SduI GDGCHC 2 cut(s) 10, 656
SfaNI GCATC 3 cut(s) 649, 1132, 1270
SfcI CTRYAG 1 cut(s) 1011
SmlI CTYRAG 1 cut(s) 1163
SmoI CTYRAG 1 cut(s) 1163
Sse9I AATT 4 cut(s) 145, 1026, 1052, 1312
SsiI CCGC 4 cut(s) 310, 323, 451, 753
SstI GAGCTC 1 cut(s) 656
StyD4I CCNGG 3 cut(s) 275, 579, 1266
StyI CCWWGG 2 cut(s) 285, 297
TaaI ACNGT 3 cut(s) 183, 189, 472
TaqI TCGA 2 cut(s) 68, 337
TasI AATT 4 cut(s) 145, 1026, 1052, 1312
TatI WGTACW 1 cut(s) 1293
TauI GCSGC 1 cut(s) 453
TfiI GAWTC 3 cut(s) 362, 571, 837
Tru1I TTAA 3 cut(s) 1056, 1164, 1205
Tru9I TTAA 3 cut(s) 1056, 1164, 1205
TscAI CASTG 2 cut(s) 364, 781
TseFI GTSAC 2 cut(s) 183, 189
TseI GCWGC 2 cut(s) 290, 447
Tsp45I GTSAC 2 cut(s) 183, 189
TspDTI ATGAA 3 cut(s) 207, 584, 905
TspRI CASTG 2 cut(s) 364, 781
Van91I CCANNNNNTGG 1 cut(s) 586
Vha464I CTTAAG 1 cut(s) 1163
XapI RAATTY 1 cut(s) 1052
XceI RCATGY 1 cut(s) 1006
XmnI GAANNNNTTC 1 cut(s) 986
Zsp2I ATGCAT 1 cut(s) 664
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.